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Abstract The clinical heterogeneity of early-stage endometrial cancer (EC) is worthy of further study to identify high-quality prognostic markers and their potential role in aggressive tumor behavior. Mutation of TP53 was considered as an important primary triage in modified molecular typing for EC, it still cannot precisely predict the prognosis of EC. After proteomic analysis of cancer and para-cancerous tissues from 24 early-stage endometrioid EC patients with different survival outcomes, 13 differentially expressed proteins were screen out while 2 proteins enriched in p53 signaling pathway were further identified by single-cell transcriptome (scRNA-seq). Interestingly, tumor necrosis factor type-1 receptor-associated protein (TRAP1) and calmodulin-regulated spectrin-associated protein family member 3 (CAMSAP3) were found to be significantly downregulated in the specific cell cluster. Expectedly, the signature genes of TRAP1low/CAMSAP3low cluster included classical oncogenes. Moreover, close cellular interactions were observed between myeloid cells and the TRAP1low/CAMSAP3low cluster after systematically elucidating their relationship with tumor microenvironment (TME). The expression of TRAP1 and CAMSAP3 was verified by immunohistochemistry. Thus, a novel prediction model combining TRAP1, CAMSAP3 and TP53 was construct by multi-omics. Compared with the area under the curve, it demonstrated a significantly improvemrnt in the diagnostic efficacy in EC patients from TCGA bank. In conclusion, this work improved the current knowledge regarding the prognosis of early-stage EC through proteomics and scRNA-seq. These findings may lead to improvements in precise risk stratification of early-stage EC patients.

Objective: In order to satisfy the enterprise's precise control the dosage of active ingredients in the new Tianlongquan pithos-stored liquor dosed with radix puerariae, siraitiae fructus and tartary buckwheat extracts. To establish a method for the simultaneous determination of 17 active ingredients (3′-hydroxypuerarin, chlorogenic acid, puerarin, epicatechin, catechins, 3′-methoxypuerarin, puerarin apioside, daidzin, ferulic acid, rutin, quercetin, isoquercitrin, kaempferol-3-O-rutinoside, mogroside IV, mogroside V, quercetin, kaempferol) by high performance liquid chromatography (HPLC). Methods: Through gradient optimization was carried out to separate each component effectively, and methodological investigations was performed for quantitation method. Results: The effective separation was achieved within 70 min on a Waters Atlantis®T3 column (4.6 mm×250 mm, 5 μm) using a optimized gradient of mobile phase consisting of acetonitrile-0.1% phosphoric acid water, and with the quantitative wavelength was 203, 250, 280 and 365 nm correspondingly. This quantitative method was of with good repeatability (relative standard deviation RSD≤4.06%), high precision (RSD≤3.86%) and reliable recovery (average recoveries ranging from 92.46% to 106.25%, RSD≤4.56%). Conclusion: The method is simple, efficient and accurate, and suitable for the quality control of the active ingredients in pithos-stored liquor.

With the rapid development of biotechnology, gene editing has become more widely used as a powerful tool to regulate plant traits directionally and efficiently. Here, we summarize the recent research progress in ornamental plant gene editing, including flower type, flower color, vase life, marker genes and other traits. We also discuss the application potential of other crop gene editing methods in ornamental plants and explore the diversity and feasibility of gene editing techniques in plant breeding to promote the molecular breeding of ornamental plants.