179 results on '"Jinling, Xu"'
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2. Continuous intrafemoral artery infusion of urokinase improves diabetic foot ulcers healing and decreases cardiovascular events in a long-term follow-up study
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Min Liu, Ling Yue, Yangyang Cheng, Jing Dong, Lin Xiang, Jiayue Tong, Junxia Zhang, Shuguang Li, Jinling Xu, Guangping Zhu, Hujun Ren, and Guangda Xiang
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Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Introduction Diabetic foot ulcer (DFU) is a disabling complication of diabetes mellitus. Here, we attempted to assess whether long-term intrafemoral artery infusion of low-dose urokinase therapy improved DFUs and decreased cardiovascular events in patients with DFUs.Research design and methods This trial was a single-center, randomized, parallel study. A total of 195 patients with DFU were randomized to continuous intrafemoral thrombolysis or conventional therapy groups. The continuous intrafemoral thrombolysis group received continuous intrafemoral urokinase injection for 7 days, and conventional therapy just received wound debridement and dressing change. Then, a follow-up of average 6.5 years was performed.Results Compared with conventional therapy, at the first 1 month of intervention stage, the ulcers achieved a significant improvement in continuous intrafemoral thrombolysis group including a complete closure (72.4% vs 17.5%), an improved ulcer (27.6% vs 25.8%), unchanged or impaired ulcer (0% vs 56.7%). During the 6.5-year follow-up, for the primary outcome of ulcer closure rate, continuous intrafemoral thrombolysis therapy obtained a better complete healing rate (HR 3.42 (95% CI 2.35 to 4.98, p
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- 2024
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3. Cytokeratin 7 Expression and Mismatch Repair Status for Survival Prediction in Patients With Low Rectal Cancer After Neoadjuvant Therapy
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Songli Shi, Mingming Zhou, Gang Wang, Jinling Xu MD, PhD, Dan Zhang, Minying Zheng MD, PhD, and Shiwu Zhang MD, PhD
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background More than half of the patients with locally advanced low rectal cancer exhibit no or minor response to nCRT. It is important to investigate the predictive and prognostic values of potential biomarkers in patients with locally advanced low rectal cancer receiving nCRT. Materials and Methods This retrospective study included 162 patients with locally advanced low rectal cancer who underwent nCRT, followed by total mesorectal excision (TME) between 2016 and 2019. Cytokeratin 7 (CK7) expression and mismatch repair (MMR) status were determined by immunohistochemistry (IHC). Categorical variables were compared using the chi-square test. Overall survival (OS) and disease-free survival (DFS) curves were estimated using the Kaplan–Meier and Cox methods. Results There were predominance significant differences in distance from anus margin ( P < .0001) and circumferential extent of the tumor ( P < .0001).CK7 positive expression was detected in 21 of the 162 patients (13%). The univariate and multivariate analysis revealed that patients whose tumors had CK7 positive expression had significantly shorter OS (HR = 3.878, P = .038; HR = 1.677 , P = .035) and DFS (HR = 3.055, P = .027;HR = 3.569 , P = .038) than those with CK7 negative expression. While patients with CK7 positive expression had a higher proportion of worse TRG compared with CK7 negative patients ( P = .001). Patients with deficient mismatch repair (dMMR) just occupied a small proportion (8.6%), but there was still a close connection between the MMR status and recurrence after TME ( P = .045). MMR status was an independent risk factor affecting the OS (HR = .307, P < .0001; HR = .123 , P < .0001) and DFS (HR = .288, P < .0001; HR = .286 , P < .0001) by univariate and multivariate analysis. But no significant difference in the proportion of TRG was observed between patients with dMMR and pMMR ( P = .920). Conclusions The result confirms negative prognostic role of CK7-positive and dMMR statuses, which have potential predictive value for neoadjuvant chemoradiotherapy response. This provides opportunity to modify individualized treatment strategies for patients with different CK7 expression levels and dMMR statuses.
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- 2023
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4. Research on Identification and Prediction of Financial Fraud of Listed Companies Based on Machine Learning.
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Jinling Xu, Jiayi Chen, and Wanqi Luo
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- 2023
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5. Advances in Sedentary Behavior among Cancer Survivors
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LU Jinling, XU Qin, HOU Hui, HU Jieman, LI Weiying, XU Xinyi, YANG Chunjing, CHEN Li
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cancer survivors ,sedentary behavior ,physical exertion ,prognosis ,internet-based intervention ,review ,Medicine - Abstract
Among cancer survivors who are characterized by poorprognosis and quality of life, there is a high prevalence of sedentary behavior, which is closely associated with adverse outcomes, requiring urgent attention. Based on this, we reviewed the latest advances in sedentary behavior among cancer survivors, including relevant theories, assessment methods, influencing factors, association with prognosis and intervention, and concluded that sedentary behavior that sedentary behavior is a major risk factor for poor prognosis in cancer survivors, and single physical activity intervention or a combined intervention of physical activity and sedentary behavior is effective intervention. Additionally, given the situation where there is an urgent need to manage sedentary behavior in this group, indigenized studies ought to be carried out actively to make some improvements in their prognosis.
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- 2023
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6. Anti‐PD‐1/Her2 Bispecific Antibody IBI315 Enhances the Treatment Effect of Her2‐Positive Gastric Cancer through Gasdermin B‐Cleavage Induced Pyroptosis
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Wu Lin, Yingzi Zhang, Yan Yang, Ben Lin, Mengjia Zhu, Jinling Xu, YiRan Chen, Weiwei Wu, Bingliang Chen, Xiangliu Chen, Jin Liu, Haohao Wang, Fei Teng, Xiongfei Yu, Haiyong Wang, Jun Lu, Quan Zhou, and Lisong Teng
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bispecific antibodies ,GSDMB ,Her2 ,immunotherapy ,pyroptosis ,Science - Abstract
Abstract The majority of patients with human epidermal growth factor receptor 2 (Her2)‐positive gastric cancer develop refractory to Her2‐targeted therapy, where upregulation of immune checkpoints plays an essential role. Herein, a recombinant fully human IgG1 bispecific antibody IBI315 targeting both PD‐1 and Her2 is developed and its antitumor efficacy as well as the underlying mechanism is investigated. IBI315 crosslinks the physical interaction between Her2‐positive tumor cells and PD‐1‐positive T cells, resulting in significantly enhanced antitumor effects compared to each parent antibody or their combination, both in vitro and in vivo mouse tumor models reconstituted with human immune cells using patient‐derived xenografts and organoids. Moreover, IBI315 treatment also induces the recruitment and activation of immune cells in tumors. Mechanistically, IBI315 triggers gasdermin B (GSDMB)‐mediated pyroptosis in tumor cells, leading to the activation and recruiments of T cells. The activated T cells secret IFNγ, enhancing GSDMB expression and establishing a positive feedback loop of T cell activation and tumor cell killing. Notably, GSDMB is found to be elevated in Her2‐positive gastric cancer cells, providing a rationale for IBI315's efficacy. IBI315 is supported here as a promising bispecific antibody‐based immunotherapy approach for Her2‐positive gastric cancer in preclinical studies, broadening the therapeutic landscape of this patient population.
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- 2023
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7. A dynamic range adjustable inverse tone mapping operator based on human visual system.
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Yufan Zhang, Wenbiao Zhou, and Jinling Xu
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- 2023
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8. A Heterogeneous FPGA-based Accelerator Design for Efficient and Low-cost Point Clouds Deep Learning Inference.
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Jinling Xu, Yonggui Wang, and Wenbiao Zhouy
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- 2022
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9. A Learnable Graph Convolutional Neural Network Model for Relation Extraction.
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Jinling Xu, Yanping Chen 0010, Yongbin Qin, and Ruizhang Huang
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- 2022
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10. Diagnostic Value of the Combined Measurement of Serum HCY and NRG4 in Type 2 Diabetes Mellitus with Early Complicating Diabetic Nephropathy
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Sheng Ding, Yi Yang, Yuming Zheng, Jinling Xu, Yangyang Cheng, Wei Wei, Fuding Yu, Li Li, Menglan Li, Mengjie Wang, Zhongjing Wang, and Guangda Xiang
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HCY ,NRG4 ,HCY/NRG4 ,diabetes ,DKD ,Medicine - Abstract
Purpose: This study aimed to investigate the value of combined detection of HCY and NRG4 in the diagnosis of early diabetic kidney disease (DKD) and to explore the association between the ratio of HCY/NRG4 and DKD. Methods: A total of 140 diabetic patients and 43 healthy people were prospectively enrolled. The plasma HCY level, NRG4 level and HCY/NRG4 of them were measured to compare their differences and analyze the correlation with DKD. The independent influencing factors of patients with DKD were screened, and the nomograph of DKD occurrence was constructed. Results: The levels of HCY and HCY/NRG4 in diabetic patients were significantly increased, while the level of NRG4 was significantly decreased (p < 0.01). The AUCs of HCY/NRG4 predicted for DKD were 0.961. HCY/NRG4 and the course of DM were independent risk factors for DKD. A predictive nomograph of DKD was constructed, and decision curve analysis (DCA) showed good clinical application value. HCY/NRG4 was positively correlated with Scr, UACR, TG, UA, BUN, TCHOL and LDL and negatively correlated with eGFR and HDL (p < 0.05). Conclusions: The level of HCY and NRG4 is closely related to the severity of DM, and combined detection of HCY/NRG4 can identify patients with DKD at an early stage.
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- 2023
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11. Computational design of highly stable and soluble alcohol dehydrogenase for NADPH regeneration
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Jinling Xu, Haisheng Zhou, Haoran Yu, Tong Deng, Ziyuan Wang, Hongyu Zhang, Jianping Wu, and Lirong Yang
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Alcohol dehydrogenase ,Computational design ,Soluble expression ,NADPH regeneration ,Chiral alcohols ,Technology ,Chemical technology ,TP1-1185 ,Biotechnology ,TP248.13-248.65 - Abstract
Abstract Nicotinamide adenine dinucleotide phosphate (NADPH), as a well-known cofactor, is widely used in the most of enzymatic redox reactions, playing an important role in industrial catalysis. However, the absence of a comparable method for efficient NADP+ to NADPH cofactor regeneration radically impairs efficient green chemical synthesis. Alcohol dehydrogenase (ADH) enzymes, allowing the in situ regeneration of the redox cofactor NADPH with high specific activity and easy by-product separation process, are provided with great industrial application potential and research attention. Accordingly, herein a NADP+-specific ADH from Clostridium beijerinckii was selected to be engineered for cofactor recycle, using an automated algorithm named Protein Repair One-stop Shop (PROSS). The mutant CbADH-6M (S24P/G182A/G196A/H222D/S250E/S254R) exhibited a favorable soluble and highly active expression with an activity of 46.3 U/mL, which was 16 times higher than the wild type (2.9 U/mL), and a more stable protein conformation with an enhanced thermal stability: Δ $${T}_{1/2}^{60\mathrm{min}}$$ T 1 / 2 60 min = + 3.6 °C (temperature of 50% inactivation after incubation for 60 min). Furthermore, the activity of CbADH-6M was up-graded to 2401.8 U/mL by high cell density fermentation strategy using recombinant Escherichia coli, demonstrating its industrial potential. Finally, the superb efficiency for NADPH regeneration of the mutant enzyme was testified in the synthesis of some fine chiral aromatic alcohols coupling with another ADH from Lactobacillus kefir (LkADH).
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- 2021
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12. Identification of Key Biomarkers and Pathways for Maintaining Cognitively Normal Brain Aging Based on Integrated Bioinformatics Analysis
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Jinling Xu, Hui Zhou, and Guangda Xiang
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brain aging ,immunity ,inflammatory ,differentially expressed genes ,hub genes ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
BackgroundGiven the arrival of the aging population has caused a series of social and economic problems, we aimed to explore the key genes underlying cognitively normal brain aging and its potential molecular mechanisms.MethodsGSE11882 was downloaded from Gene Expression Omnibus (GEO). The data from different brain regions were divided into aged and young groups for analysis. Co-expressed differentially expressed genes (DEGs) were screened. Functional analysis, protein–protein interaction (PPI) network, microRNA (miRNA)-gene, and transcription factor (TF)-gene networks were performed to identify hub genes and related molecular mechanisms. AlzData database was used to elucidate the expression of DEGs and hub genes in the aging brain. Animal studies were conducted to validate the hub genes.ResultsCo-expressed DEGs contained 7 upregulated and 87 downregulated genes. The enrichment analysis indicated DEGs were mainly involved in biological processes and pathways related to immune-inflammatory responses. From the PPI network, 10 hub genes were identified: C1QC, C1QA, C1QB, CD163, FCER1G, VSIG4, CD93, CD14, VWF, and CD44. CD44 and CD93 were the most targeted DEGs in the miRNA-gene network, and TIMP1, HLA-DRA, VWF, and FGF2 were the top four targeted DEGs in the TF-gene network. In AlzData database, the levels of CD44, CD93, and CD163 in patients with Alzheimer’s disease (AD) were significantly increased than those in normal controls. Meanwhile, in the brain tissues of cognitively normal mice, the expression of CD44, CD93, and CD163 in the aged group was significantly lower than those in the young group.ConclusionThe underlying molecular mechanisms for maintaining healthy brain aging are related to the decline of immune-inflammatory responses. CD44, CD93, and CD 163 are considered as potential biomarkers. This study provides more molecular evidence for maintaining cognitively normal brain aging.
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- 2022
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13. Research on Identification and Prediction of Financial Fraud of Listed Companies Based on Machine Learning
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Jinling, Xu, primary, Jiayi, Chen, additional, and Wanqi, Luo, additional
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- 2023
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14. Idiopathic cocoon abdomen with congenital colon malrotation: a case report and review of the literature
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Hui Zhou, Jinling Xu, Xingwang Xie, and Jiantao Han
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Cocoon abdomen ,Colon malrotation ,Intestinal obstruction ,Diagnosis ,Treatment ,Surgery ,RD1-811 - Abstract
Abstract Background Cocoon abdomen is a relatively rare abdominal disease characterized by the total or partial encasement of the small intestinal by a dense fibro-collagenous membrane. Case presentation We reported an unusual case of idiopathic cocoon abdomen with congenital colon malrotation. Laparotomy and sac release were performed on the patient. The patient was no recurrence 6 months after operation. A literature review was also performed. Conclusion Preoperative diagnosis of abdominal cocoon is difficult. A careful history, physical examination and appropriate radiology may be helpful in making a definitive diagnosis. If conservative treatment can’t relieve symptoms effectively, surgery is currently considered to be important in the management of this disease.
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- 2020
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15. Field-testing of a rapid survey method to assess the prevalence and causes of hearing loss in Gao’an, Jiangxi province, China
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Tess Bright, Xin Shan, Jinling Xu, Jianguo Liang, Baixiang Xiao, Robbert Ensink, Islay Mactaggart, Sarah Polack, and Jennifer L. Y. Yip
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China ,Hearing loss ,Rapid assessment ,Population-based survey ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The Rapid Assessment of Hearing Loss (RAHL) survey protocol aims to measure the prevalence and causes of hearing loss in a low cost and rapid manner, to inform planning of ear and hearing services. This paper reports on the first field-test of the RAHL in Gao’an County, Jiangxi Province, China. This study aimed to 1) To report on the feasibility of RAHL; 2) report on the estimated prevalence and causes of hearing loss in Gao’an. Methods A cross-sectional population-based survey was conducted in September–October 2018. Forty-seven clusters in Gao’an County were selected using probability-proportionate-to-size sampling. Within clusters, compact segment sampling was conducted to select 30 people aged 50+. A questionnaire was completed covering sociodemographics, hearing health, and risk factors. Automated pure-tone audiometry was completed for all participants, using smartphone-based audiometry (hearTest), at 0.5, 1, 2, 4 kHz (kHz). All participants had their ears examined by an Ear Nose and Throat (ENT) doctor, using otoscopy, and probable causes of hearing loss assigned. Prevalence estimates were age and sex standardised to the Jiangxi population. Feasibility of a cluster size of 30 was examined by assessing the response rate, and the proportion of clusters completed in 1 day. Results 1344 of 1421 eligible participants completed the survey (94.6%). 100% of clusters were completed in 1 day. The survey was completed in 4.5 weeks. The prevalence of moderate or greater hearing loss (pure-tone average of 0.5, 1, 2, 4 kHz of > = 41dBHL in the better ear) was 16.3% (95% CI = 14.3, 18.5) and for any level of hearing loss (pure-tone average of > = 26dBHL in the better ear) the prevalence was 53.2% (95% CI = 49.2, 57.1). The majority of hearing loss was due to acquired sensorineural causes (91.7% left; 92.1% right). Overall 54.0% of the population aged 50+ (108,000 people) are in need of diagnostic audiology services, 3.4% were in need of wax removal (7000 people), and 4.8% were in need of surgical services (9500 people). Hearing aid coverage was 0.4%. Conclusion The RAHL survey protocol is feasible, demonstrated through the number of people examined per day, and the high response rate. The survey was completed in a much shorter period than previous all-age surveys in China. Some remaining challenges included assignment of causes of probable sensorineural loss. The data obtained from this survey can be used to scale-up hearing services in Gao’an.
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- 2020
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16. Factors influencing the self-reported sense of deviation in adults with successful surgical outcomes for strabismus
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Na Ji, Meiping Xu, Huanyun Yu, Jinling Xu, and Xinping Yu
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Strabismus ,Surgery ,Self-sense ,Outcome ,Deviation ,Ophthalmology ,RE1-994 - Abstract
Abstract Background To determine whether a sense of deviation remains in adults with successful motor alignment who fulfil diplopia criteria after surgery and to examine the factors associated with this judgement. Methods This was a retrospective study. Adult patients defined as having a successful outcome based on more than 1 year of post-operative follow-up visits were included in the study. The sense of deviation was determined at the last visit. Pre- and post-operative deviation and characteristics including age, gender, education level, occupation, diagnosis, size of deviation, extraocular movement (EOM), binocular function, and health-related quality of life (HRQOL) were recorded. Results In total, 22 (24%) of the 91 adults with successful surgical outcomes reported a sense of deviation. No significant differences were noted between subjects with and without a sense of deviation regarding patient demographics, pre- and post-operative deviation, changes in deviation, sensory fusion or EOM. Subjects with a sense of deviation had an increased prevalence of and larger post-operative vertical deviation, poorer stereo function, and lower HRQOL scores than those with no sense of deviation. The presence of post-operative vertical deviation was associated with a sense of deviation. Conclusions Approximately one-fourth (24%) of adults defined as having successful surgical outcomes who still had a sense of deviation exhibited worse stereo function, higher vertical deviation size and lower HRQOL scores. The presence of 3 to 5 prism dioptres(pd) of vertical deviation would be the main factor associated with a sense of deviation post-operatively.
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- 2020
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17. Molecular characterization of three intestinal protozoans in hospitalized children with different disease backgrounds in Zhengzhou, central China
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Fuchang Yu, Dongfang Li, Yankai Chang, Yayun Wu, Zhenxin Guo, Liting Jia, Jinling Xu, Junqiang Li, Meng Qi, Rongjun Wang, and Longxian Zhang
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Cryptosporidium ,Giardia duodenalis ,Enterocytozoon bieneusi ,Epidemiology ,Children ,China ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Cryptosporidium spp. and Giardia duodenalis are major intestinal pathogens that can cause diarrheal diseases in humans, especially children. Enterocytozoon bieneusi is another parasite which can cause gastrointestinal tract disorders, with diarrhea being the main clinical symptom. However, few genetic studies of these parasites in pediatric inpatients in China have been published. Methods To assess the genetic characteristics and epidemiological status of these parasites, a total of 2284 fecal samples were collected from children in the pediatric departments of three hospitals in Zhengzhou, central China, and screened for these protozoans with PCR, based on the small subunit ribosomal RNA (SSU rRNA) genes of Cryptosporidium spp. and G. duodenalis and the internal transcribed spacer (ITS) of E. bieneusi. Results Six (0.26%), 14 (0.61%), and 27 (1.18%) of the samples were positive for Cryptosporidium spp., G. duodenalis and E. bieneusi, respectively. Of the 12 successfully sequenced G. duodenalis isolates, four were identified as assemblage A and eight as assemblage B. In subtype and multilocus genotype (MLG) analyses, C. parvum IIdA19G1 (n = 4) and two novel G. duodenalis MLGs belonging to subassemblage AII (n = 3) and BIV (n = 5) were successfully identified. The E. bieneusi isolates included genotypes D (n = 17), J (n = 2), PigEBITS7 (n = 1), BEB6 (n = 1), and CM8 (n = 1). This is the first report of C. parvum subtype IIdA19G1 in HIV-negative children and E. bieneusi genotype CM8 in humans. Conclusions The dominance of zoonotic C. parvum subtype IIdA19G1 indicates that this parasite is turning into zoonotic origin from human-to-human transmission. The phylogenetic analysis also revealed the zoonotic origins and anthroponotic transmission potential of G. duodenalis and E. bieneusi, suggesting more efforts must be made to minimize the threat these pathogens pose to public health.
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- 2019
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18. Screening and Prognostic Value of Methylated Septin9 and its Association With Clinicopathological and Molecular Characteristics in Colorectal Cancer
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Jie Sun, Jinling Xu, Chao Sun, Minying Zheng, Yuwei Li, Siwei Zhu, and Shiwu Zhang
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colorectal cancer ,mSEPT9 ,prognosis ,dMMR ,genetic mutation ,Biology (General) ,QH301-705.5 - Abstract
Screening of CRC continues to show poor compliance of endoscopy examination. The detection of mSEPT9 in peripheral blood is among the safe and simple early screening methods for CRC. The issue of how to elucidate whether detection of mSEPT9 in peripheral blood can effectively improve compliance of endoscopy and increase the early diagnosis rate of CRC and the relationship between levels of mSEPT9 in the peripheral blood and clinical stage, pathological classification, and expression of characteristic molecules in CRC remains unsolved. A total of 7759 individuals participated in the study that was performed using a questionnaire for screening of high-risk CRC. The endoscopic detection compliance of individuals with high-risk CRC who underwent the fecal occult blood test (FOBT) or mSEPT9 test was compared based on the results of the questionnaire. Additionally, correlation of mSEPT9 levels in the peripheral blood with clinicopathological features, mutation status of TP53, mismatch repair deficiency (dMMR), and KRAS/NRAS/BRAF/PIK3CA genotype was analyzed, and association of biomarkers with cancer-specific survival (CSS) and time to recurrence (TTR) was compared. We also detected levels of mSEPT9 in the peripheral blood of patients with CRC 7 days after surgery and compared the prognostic value of mSEPT9 with CEA. Results of our study showed that the mSEPT9 test could improve compliance of endoscopy and indicated a higher percentage of patients with positive mSEPT9 willing to undergo endoscopy detection than in those with positive FOBT. The specificity and sensitivity of mSEPT9 were better than that of FOBT for the detection of CRC. mSEPT9 was associated with the TNM stage, dMMR, and mutations in TP53, BRAF, and PIK3CA. A Ct value of mSEPT9 ≤ 37.5 was significantly related to poor CSS. mSEPT9 could affect association of dMMR and BRAF and PIK3CA mutations with CSS in a specific stage of CRC. The positive rate of mSEPT9 after surgery was found to correlate with poor TTR, and sensitivity was higher than CEA. The combination of mSEPT9 with CEA had a better prognostic value than that of mSEPT9 alone. The level of mSEPT9 was related to dMMR, mutations in TP53, BRAF, and PIK3CA, and was an effective biomarker for the prognosis of patients with CRC.
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- 2021
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19. A Randomized Clinical Trial Comparing Eyetronix Flicker Glass and Patching for Treatment of Amblyopia in Children Reveals Similar Improvements in Vision
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Seung Hyun Min, Shijia Chen, Jinling Xu, Bingzhen Chen, Hui Chen, Yuwen Wang, Jiawei Zhou, and Xudong Yu
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amblyopia ,eyetronix flicker glass ,randomized controlled trial ,patching ,visual acuity ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
PurposeRecently, Eyetronix Flicker Glass (EFG) has been introduced as a novel treatment for amblyopia. It alternatively deprives the visual input of each eye rapidly (e.g., 7 Hz). However, whether it is comparable with standard patching therapy is unclear. In this randomized clinical trial, we evaluate the efficacy of an EFG therapy as treatment for amblyopia in children and compare it to the patching therapy.MethodsWe tested 31 children (aged 4–13 years) with amblyopia. They were assigned into one of the two treatment groups and were treated for 12 weeks. The first group was treated with EFG for 1 h/day (Flicker Group) and the latter with a standard patch (Patching Group) for 2 h/day. We designated changes from baseline in best-corrected visual acuity (BCVA) of the amblyopic eye as our primary outcome. Changes from baseline in other visual outcomes, such as contrast sensitivity, stereopsis, and fusional vergence range were measured as secondary outcome.ResultsBCVA improved significantly at 12 weeks relative to baseline in both the Flicker (0.13 ± 0.11 logMAR; mean ± SD) and Patching Groups (0.21 ± 0.14 logMAR). However, the improvements were not significantly different between groups (p = 0.13). Contrast sensitivity also significantly improved at 3 and 12 cycles/degree between baseline and 12 weeks in both groups (p’s < 0.05). However, stereopsis and fusion range did not improve significantly in both groups.ConclusionAn EFG therapy and patching improved BCVA similarly for children with amblyopia at 12 weeks. Both therapies improved the contrast sensitivity at 3 and 12 cycles per degree (cpd); however, only patching improved the contrast sensitivity at 6 cpd. Both therapies did not benefit binocular visual functions (stereopsis and fusional vergence range). We believe that EFG can be an additional choice for therapy.Clinical Trial Registrationchictr.org number: ChiCTR2000034436.
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- 2021
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20. Oolong tea polysaccharide and polyphenols prevent obesity development in Sprague–Dawley rats
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Tao Wu, Jinling Xu, Yijun Chen, Rui Liu, and Min Zhang
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oolong tea ,anti-obesity ,polysaccharide ,polyphenols ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background: Several studies have evaluated the effects of oolong tea extracts on obesity. However, only few studies focused on the anti-obesity effect of specific components of oolong tea. Objective: This study investigated the specific anti-obesity capabilities of oolong tea polysaccharide (TPS) and tea polyphenols (TPP) in high-fat diet-induced Sprague–Dawley rats. Methods: Oolong tea water extract, TPS, TPP, and polysaccharide mixed with polyphenol (TPSM) given at doses of 400 or 800 mg/kg were administered to rats fed with high-fat diet for 6 weeks to explore the anti-obesity effects of the treatments. Results: TPS and TPP were responsible for the suppressive effect on body fat accumulation. TPSM exhibited the highest effect on body weight reduction, and TPS and TPP effectively reduced serum leptin levels and significantly improved blood lipid and antioxidant levels. Moreover, microarray analysis of hepatic and adipose gene expression profiles revealed that TPP and TPS inhibited obesity through effects on the pathways of fatty acid biosynthesis, steroid hormone biosynthesis, unsaturated fatty acid biosynthesis, fatty acid elongation, glycerolipid metabolism, and glycerophospholipid metabolism. Conclusions: TPSM might be a potential therapy for the treatment of obesity.
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- 2018
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21. Computational Redesign of the Substrate Binding Pocket of Glutamate Dehydrogenase for Efficient Synthesis of Noncanonical <scp>l</scp>-Amino Acids
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Ziyuan Wang, Haisheng Zhou, Haoran Yu, Zhongji Pu, Jinling Xu, Hongyu Zhang, Jianping Wu, and Lirong Yang
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General Chemistry ,Catalysis - Published
- 2022
22. COX-2 Forms Regulatory Loop with YAP to Promote Proliferation and Tumorigenesis of Hepatocellular Carcinoma Cells
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Guanglin Xu, Ying Wang, Weijie Li, Yuanyuan Cao, Jinling Xu, Ziwei Hu, Yaping Hao, Li Hu, and Yawen Sun
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
COX-2 and YAP are shown to be highly associated with hepatocellular carcinoma (HCC) and frequently upregulated during tumor formation. However, despite their importance, whether there is a mutual interaction between COX-2 and YAP and how they regulate each other are not clear. In this paper, we showed that COX-2 overexpression in HCC cell lines resulted in increased levels of YAP mRNA, protein, and its target genes. COX-2 promoted proliferation of HCC cell lines, and knockdown of YAP antagonized this effect. In addition, our results indicated that EP2 and Wnt/β-Catenin mediate the transcriptional induction of YAP by COX-2. On the other hand, YAP increased COX-2 expression at the level of transcription requiring intact TEAD binding sites in the COX-2 promoter. Collectively, these findings indicated that COX-2 is not only a stimulus of YAP but also a target of Hippo-YAP pathway, thus forming a positive feedback circuit, COX-2-PGE2-EP2-Gαs-β-catenin-YAP-COX-2. In a further study, we showed that inhibition of YAP and COX-2 acted synergistically and more efficiently reduced the growth of HCC cells and tumor formation than either of them alone, suggesting that dual governing of YAP and COX-2 may lead to the discovery of promising therapeutic strategies for HCC patients via blocking this positive feedback loop.
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- 2018
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23. A Feature Combination-Based Graph Convolutional Neural Network Model for Relation Extraction
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Jinling Xu, Yanping Chen, Yongbin Qin, Ruizhang Huang, and Qinghua Zheng
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relation extraction ,feature combination ,graph neural network ,nlp ,Mathematics ,QA1-939 - Abstract
The task to extract relations tries to identify relationships between two named entities in a sentence. Because a sentence usually contains several named entities, capturing structural information of a sentence is important to support this task. Currently, graph neural networks are widely implemented to support relation extraction, in which dependency trees are employed to generate adjacent matrices for encoding structural information of a sentence. Because parsing a sentence is error-prone, it influences the performance of a graph neural network. On the other hand, a sentence is structuralized by several named entities, which precisely segment a sentence into several parts. Different features can be combined by prior knowledge and experience, which are effective to initialize a symmetric adjacent matrix for a graph neural network. Based on this phenomenon, we proposed a feature combination-based graph convolutional neural network model (FC-GCN). It has the advantages of encoding structural information of a sentence, considering prior knowledge, and avoiding errors caused by parsing. In the experiments, the results show significant improvement, which outperform existing state-of-the-art performances.
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- 2021
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24. YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer
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Wei Li, Yuanyuan Cao, Jinling Xu, Ying Wang, Weijie Li, Qian Wang, Ziwei Hu, Yaping Hao, Li Hu, Yawen Sun, Guanglin Xu, and Guizhen Ao
- Subjects
Yap ,COX-2 ,Colorectal cancer ,Resistance ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Chemotherapy resistance remains a major challenge in cancer treatment. COX-2 (cyclooxygenase 2) is involved in drug resistance and poor prognosis of many neoplastic diseases or cancers. However, investigations identifying new modulators of COX-2 pathway and searching for new chemicals targeting these valid resistant biomarkers are still greatly needed. Methods HCT15, HCT-116, HT-29, COLO205, FHC, IMCE, SW480 cell lines were used to detect the expression of YAP and COX-2. Site-directed mutagenesis, luciferase reporter analysis and ChIP assay were used to test whether YAP activated COX-2 transcription through interaction with TEAD binding sites in the promoter of COX-2. Cell line models exhibiting overexpression or knockdown of some genes were generated using transfection agents. Coimmunoprecipitation was used to detect protein mutual interaction. mRNA and protein levels were measured by qRT-PCR and western blot respectively. Results Here, we reported that both YAP and COX-2 were overexpressed in colorectal cancer cells. YAP increased COX-2 expression at the level of transcription requiring intact TEAD binding sites in the COX-2 promoter. YAP conferred drug resistance through COX-2 and its related effectors such as MCL, MDR, Survivin. GCCSysm-4 (G-4), a YAP and COX-2 inhibitor, effectively inhibited both YAP and COX-2 activation, induced apoptosis and decreased viability in Taxol-resistant cells. Inhibition of YAP and COX-2 acted synergistically and more efficiently reduced the resistance of CRC cells than either of them alone. Conclusions Our data provide new mechanisms that YAP is a new upstream regulator of COX-2 pathway and plays an important role in conferring resistance in CRC cells. G-4, targeting YAP-COX-2, may be a novel valuable strategy to combat resistance in CRC.
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- 2017
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25. Identification of RPGR ORF15 mutation for X-linked retinitis pigmentosa in a large Chinese family and in vitro correction with prime editor
- Author
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Xiujuan Lv, Zheng Zheng, Xiao Zhi, Yilin Zhou, Jineng Lv, Yue Zhou, Binrong Wu, Sixiu Liu, Wei Shi, Zongming Song, Jinling Xu, Jia Qu, Dan Xu, and Feng Gu
- Subjects
Genetics ,Molecular Medicine ,Molecular Biology - Abstract
X-linked retinitis pigmentosa (XLRP) is the most severe form of Retinitis Pigmentosa (RP) and one of the leading causes of blindness in the world. Currently, there is no effective treatment for RP. In the present study, we recruited a XLRP family and identified a 4 bp deletion mutation (c. 2234_2237del) in RPGR ORF15 with Sanger sequencing, which was located in the exact same region as the missing XES (X chromosome exome sequencing) coverage. Then, we generated cell lines harboring the identified mutation and corrected it via enhanced prime editing system (ePE). Collectively, Sanger sequencing identified a pathogenic mutation in RPGR ORF15 for XLRP which was corrected with ePE. This study provides a valuable insight for genetic counseling of the afflicted family members and prenatal diagnosis, also paves a way for applying prime editing based gene therapy in those patients.
- Published
- 2022
26. A dynamic range adjustable inverse tone mapping operator based on human visual system
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Yufan Zhang, Wenbiao Zhou, and Jinling Xu
- Subjects
Computer Vision and Pattern Recognition ,Computer Graphics and Computer-Aided Design ,Software - Published
- 2022
27. A Convenient and Robust Test to Quantify Interocular Suppression for Children With Amblyopia
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Hui Chen, Zhifen He, Jinling Xu, Yu Mao, Yunjie Liang, Danli Lin, Meiping Xu, Zhiyue Dai, Xiaoxin Chen, Jiawei Zhou, and Xinping Yu
- Subjects
Psychology ,BF1-990 - Abstract
Interocular suppression was quantified by the interocular luminance difference that was needed when the two eyes were balanced in discriminating a black–white stripe formed butterfly stimulus, which was dichoptically presented through polarized glasses. Stronger interocular suppression was found in amblyopes than that in controls at both the near (33 cm, 0.95 ± 1.00 vs. 0.14 ± 0.18, p
- Published
- 2019
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28. A Learnable Graph Convolutional Neural Network Model for Relation Extraction
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Jinling Xu, Yanping Chen, Yongbin Qin, and Ruizhang Huang
- Published
- 2023
29. Adsorption Behavior of Soil Fulvic Acid on Crystal Faces of Clay Minerals: Described by Cd-Music Model
- Author
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Hongfeng Chen, Mingxia Hou, Zhouyang He, Yu Liang, Jinling Xu, and Wenfeng Tan
- Published
- 2023
30. Enhancing thermostability of lipase from Pseudomonas alcaligenes for producing <scp>l</scp>-menthol by the CREATE strategy
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Zhonglang Yu, Haoran Yu, Jinling Xu, Zhe Wang, Ziyuan Wang, Tingting Kang, Kaitong Chen, Zhongji Pu, Jianping Wu, Lirong Yang, and Gang Xu
- Subjects
Catalysis - Abstract
Rapid enhancement of the thermostability of enzymes by the CREATE strategy.
- Published
- 2022
31. Contamination and source apportionment of metals in urban road dust (Jinan, China) integrating the enrichment factor, receptor models (FA-NNC and PMF), local Moran's index, Pb isotopes and source-oriented health risk
- Author
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Xiaoyu Wang, Enfeng Liu, Mengxia Yan, Shuwei Zheng, Ying Fan, Yingxue Sun, Zijun Li, and Jinling Xu
- Subjects
Environmental Engineering ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Published
- 2023
32. Identifying potential signatures for atherosclerosis in the context of predictive, preventive, and personalized medicine using integrative bioinformatics approaches and machine-learning strategies
- Author
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Jinling Xu, Hui Zhou, Yangyang Cheng, and Guangda Xiang
- Subjects
Health Policy ,Biochemistry (medical) ,Drug Discovery - Abstract
Atherosclerosis is a major contributor to morbidity and mortality worldwide. Although several molecular markers associated with atherosclerosis have been developed in recent years, the lack of robust evidence hinders their clinical applications. For these reasons, identification of novel and robust biomarkers will directly contribute to atherosclerosis management in the context of predictive, preventive, and personalized medicine (PPPM). This integrative analysis aimed to identify critical genetic markers of atherosclerosis and further explore the underlying molecular immune mechanism attributing to the altered biomarkers.Gene Expression Omnibus (GEO) series datasets were downloaded from GEO. Firstly, differential expression analysis and functional analysis were conducted. Multiple machine-learning strategies were then employed to screen and determine key genetic markers, and receiver operating characteristic (ROC) analysis was used to assess diagnostic value. Subsequently, cell-type identification by estimating relative subsets of RNA transcript (CIBERSORT) and a single-cell RNA sequencing (scRNA-seq) data were performed to explore relationships between signatures and immune cells. Lastly, we validated the biomarkers' expression in human and mice experiments.A total of 611 overlapping differentially expressed genes (DEGs) included 361 upregulated and 250 downregulated genes. Based on the enrichment analysis, DEGs were mapped in terms related to immune cell involvements, immune activating process, and inflaming signals. After using multiple machine-learning strategies, dehydrogenase/reductase 9 (DHRS9) and protein tyrosine phosphatase receptor type J (PTPRJ) were identified as critical biomarkers and presented their high diagnostic accuracy for atherosclerosis. From CIBERSORT analysis, both DHRS9 and PTPRJ were significantly related to diverse immune cells, such as macrophages and mast cells. Further scRNA-seq analysis indicated DHRS9 was specifically upregulated in macrophages of atherosclerotic lesions, which was confirmed in atherosclerotic patients and mice.Our findings are the first to report the involvement of DHRS9 in the atherogenesis, and the proatherogenic effect of DHRS9 is mediated by immune mechanism. In addition, we confirm that DHRS9 is localized in macrophages within atherosclerotic plaques. Therefore, upregulated DHRS9 could be a novel potential target for the future predictive diagnostics, targeted prevention, patient stratification, and personalization of medical services in atherosclerosis.The online version contains supplementary material available at 10.1007/s13167-022-00289-y.
- Published
- 2022
33. Quantitative Characterization of the Site Density and the Charged State of Functional Groups on Biochar
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Wei Zhao, Wenfeng Tan, Luuk K. Koopal, Jinling Xu, Mengge Zhou, Juan Xiong, Chang Chen, and Mingxia Wang
- Subjects
Renewable Energy, Sustainability and the Environment ,Chemistry ,General Chemical Engineering ,Proton dissociation ,Charge density ,02 engineering and technology ,General Chemistry ,State (functional analysis) ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Characterization (materials science) ,Chemical physics ,Biochar ,Environmental Chemistry ,0210 nano-technology - Abstract
Charge density and the proton dissociation state of surface groups play a crucial role in understanding biochar’s sustainable applications in the environment. Such knowledge is still limited due to...
- Published
- 2021
34. Response to: Comment on 'Exotropia Is the Main Pattern of Childhood Strabismus Surgery in the South of China: A Six-Year Clinical Review'
- Author
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Xinping Yu, Zhouduo Ji, Huanyun Yu, Meiping Xu, and Jinling Xu
- Subjects
Ophthalmology ,RE1-994 - Published
- 2017
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- View/download PDF
35. Thickness of retinal layers in the foveas of children with anisometropic amblyopia.
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Wuhe Chen, Jinling Xu, Jinjing Zhou, Zhouqun Gu, Shenghai Huang, Heming Li, Zhuoer Qin, and Xinping Yu
- Subjects
Medicine ,Science - Abstract
To use highly precise spectral-domain optical coherence tomography (SD-OCT) to determine whether there were structural abnormalities in the layers of different regions of the fovea in children with anisometropic amblyopia.Eighteen children (mean age 7.8 years old; range 5-11 years) with unilateral anisometropic amblyopia and 18 age-matched control subjects participated. Foveal thickness was measured with an enhanced depth imaging system, SD-OCT and segmented into layers using custom developed software. The thickness of each layer of the fovea was compared among amblyopic eyes, fellow eyes and control eyes with optical magnification correction for axial length and statistical correction for age and sex.The total thickness and each intra-ocular layer of the central fovea were the same for each group. However, the amblyopic eyes were significantly thicker than the normal control eyes in 2 of 4 quadrants of the peripheral retina. Exploring intra-retinal layers in these two quadrants, the nasal nerve fiber layer (NFL) and inferior inner nuclear layer (INL)were significantly thicker in amblyopic eyes than in control eyes (p = 0.01 and 0.012, respectively, by ANCOVA).The SD-OCT data revealed marginal differences in some foveal layers at peripheral locations and indicated that structural differences might exist between individuals with amblyopia and visually normal control subjects. However, the differences were scattered and represented no identifiable pattern. More studies with large samples and precise locations of the retinal layers must be performed to extend the present results.
- Published
- 2017
- Full Text
- View/download PDF
36. Brown adipose tissue-derived Nrg4 alleviates endothelial inflammation and atherosclerosis in male mice
- Author
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Lingfeng Shi, Yixiang Li, Xiaoli Xu, Yangyang Cheng, Biying Meng, Jinling Xu, Lin Xiang, Jiajia Zhang, Kaiyue He, Jiayue Tong, Junxia Zhang, Lingwei Xiang, and Guangda Xiang
- Subjects
Male ,Inflammation ,Mice ,Adipose Tissue, Brown ,Physiology (medical) ,Endocrinology, Diabetes and Metabolism ,Internal Medicine ,Animals ,Endothelial Cells ,Cell Biology ,Endothelium ,Atherosclerosis ,Neuregulins - Abstract
Brown adipose tissue (BAT) activity contributes to cardiovascular health by its energy-dissipating capacity but how BAT modulates vascular function and atherosclerosis through endocrine mechanisms remains poorly understood. Here we show that BAT-derived neuregulin-4 (Nrg4) ameliorates atherosclerosis in mice. BAT-specific Nrg4 deficiency accelerates vascular inflammation and adhesion responses, endothelial dysfunction and apoptosis and atherosclerosis in male mice. BAT-specific Nrg4 restoration alleviates vascular inflammation and adhesion responses, attenuates leukocyte homing and reduces endothelial injury and atherosclerosis in male mice. In endothelial cells, Nrg4 decreases apoptosis, inflammation and adhesion responses induced by oxidized low-density lipoprotein. Mechanistically, protein kinase B (Akt)–nuclear factor-κB signaling is involved in the beneficial effects of Nrg4 on the endothelium. Taken together, the results reveal Nrg4 as a potential cross-talk factor between BAT and arteries that may serve as a target for atherosclerosis.
- Published
- 2022
37. Author Correction: Brown adipose tissue-derived Nrg4 alleviates endothelial inflammation and atherosclerosis in male mice
- Author
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Lingfeng Shi, Yixiang Li, Xiaoli Xu, Yangyang Cheng, Biying Meng, Jinling Xu, Lin Xiang, Jiajia Zhang, Kaiyue He, Jiayue Tong, Junxia Zhang, Lingwei Xiang, and Guangda Xiang
- Subjects
Physiology (medical) ,Endocrinology, Diabetes and Metabolism ,Internal Medicine ,Cell Biology - Published
- 2022
38. Phosphate Ion‐Functionalized CoS with Hexagonal Bipyramid Structures from a Metal–Organic Framework: Bifunctionality towards Supercapacitors and Oxygen Evolution Reaction
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Guancheng Xu, Jiahui Jiang, Weiwei Wang, Li Zhang, and Jinling Xu
- Subjects
Tafel equation ,010405 organic chemistry ,Organic Chemistry ,Oxygen evolution ,chemistry.chemical_element ,General Chemistry ,Overpotential ,010402 general chemistry ,Electrochemistry ,01 natural sciences ,Cobalt sulfide ,Catalysis ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Metal-organic framework ,Cobalt - Abstract
To solve energy-related environmental problems and the energy crisis, efficient electrochemical materials have been developed as alternative energy storage and conversion systems. Abundant transition metals and their sulfides are attractive electrochemical materials. Herein, we report an efficient phosphorization strategy, which improves the overall electrochemical performance of metal sulfides. In detail, CoS hexagonal bipyramids were synthesized through simple calcination combined with in situ sulfurization of a cobalt-based metal-organic framework template, and then phosphate ion-functionalized CoS (P-CoS) was prepared through a phosphorization reaction. P-CoS exhibited outstanding electrochemical activity as both supercapacitor electrode and oxygen evolution reaction (OER) catalyst. Supercapacitors based on CoS and P-CoS as the electrodes had high specific capacitances of 304 and 442 F g-1 , respectively, and remained stable for over 10 000 cycles at 5 A g-1 . For OER, P-CoS showed a current density of 10 mA cm-2 at an overpotential of 340 mV, with a small Tafel slope. In conclusion, functionalizing CoS with phosphate ions is a promising method for enhancing chemical reactivity and accelerating ion and electron transfer.
- Published
- 2020
39. Application of Astronomical Dim Target Processing Algorithm in UAV Detection
- Author
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Yao Li, MengYuan Yao, Liang Shi, ChangZheng Ma, Zheng Kong, ZiWen Wang, Xiang Li, JinLing Xu, and YongQiang Yao
- Published
- 2022
40. Association of Atherogenic Index of Plasma With Retinopathy and Nephropathy in Adult Patients With Type 2 Diabetes Mellitus Aged 18 Years
- Author
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Jinling Xu, Hui Zhou, and Guangda Xiang
- Subjects
Endocrinology ,Endocrinology, Diabetes and Metabolism ,Internal Medicine ,General Medicine - Abstract
In this study we assessed the atherogenic index of plasma (AIP) in relation to retinopathy and nephropathy in patients with type 2 diabetes mellitus (T2DM).In this cross-sectional study there were 4,358 inpatients with T2DM. The patients were divided into 4 groups: T2DM without complications (DM group), T2DM complicated with retinopathy alone and nephropathy alone (DR-alone and DN-alone groups) and T2DM complicated with both DR and DN (DRN group). AIP was calculated by the formula of log [triglyceride / high-density lipoprotein cholesterol].AIPs in the DR-alone, DN-alone and DRN groups were significantly higher than in the DM group, with the DRN group having the highest AIP level. Moreover, patients with proliferative DR had higher AIPs than patients with nonproliferative DR in the DR-alone and DRN groups, and patients with macroalbuminuria had higher AIPs than patients with microalbuminuria in DN-alone and DRN groups. The highest AIP quartile group had the highest proportion of DRN compared with the other quartile groups. The DRN group had a maximal area under the curve (AUC) for AIP on receiver operating characteristic curve analysis (AUC=0.735). In the subgroup analyses by age, the AUCs of patients65 years of age were all greater than those of patients ≥65 years of age. Logistic regression analysis showed that AIP had the highest correlation with age65 years in individuals with DRN, and this association remained significant after adjustment with 3 models.AIP is positively associated with both occurrence and severity of diabetic microvascular complications. It can predict their presence in T2DM, especially in those65 years of age with DRN.
- Published
- 2021
41. Identification of Key Biomarkers and Pathways for Maintaining Cognitively Normal Brain Aging Based on Integrated Bioinformatics Analysis
- Author
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Jinling Xu, Hui Zhou, and Guangda Xiang
- Subjects
Aging ,Cognitive Neuroscience - Abstract
BackgroundGiven the arrival of the aging population has caused a series of social and economic problems, we aimed to explore the key genes underlying cognitively normal brain aging and its potential molecular mechanisms.MethodsGSE11882 was downloaded from Gene Expression Omnibus (GEO). The data from different brain regions were divided into aged and young groups for analysis. Co-expressed differentially expressed genes (DEGs) were screened. Functional analysis, protein–protein interaction (PPI) network, microRNA (miRNA)-gene, and transcription factor (TF)-gene networks were performed to identify hub genes and related molecular mechanisms. AlzData database was used to elucidate the expression of DEGs and hub genes in the aging brain. Animal studies were conducted to validate the hub genes.ResultsCo-expressed DEGs contained 7 upregulated and 87 downregulated genes. The enrichment analysis indicated DEGs were mainly involved in biological processes and pathways related to immune-inflammatory responses. From the PPI network, 10 hub genes were identified: C1QC, C1QA, C1QB, CD163, FCER1G, VSIG4, CD93, CD14, VWF, and CD44. CD44 and CD93 were the most targeted DEGs in the miRNA-gene network, and TIMP1, HLA-DRA, VWF, and FGF2 were the top four targeted DEGs in the TF-gene network. In AlzData database, the levels of CD44, CD93, and CD163 in patients with Alzheimer’s disease (AD) were significantly increased than those in normal controls. Meanwhile, in the brain tissues of cognitively normal mice, the expression of CD44, CD93, and CD163 in the aged group was significantly lower than those in the young group.ConclusionThe underlying molecular mechanisms for maintaining healthy brain aging are related to the decline of immune-inflammatory responses. CD44, CD93, and CD 163 are considered as potential biomarkers. This study provides more molecular evidence for maintaining cognitively normal brain aging.
- Published
- 2021
42. Research on Intelligent Collaboration of Two-Layer Self-Organizing Network
- Author
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Ting Wang, Xiaodong Cheng, Jinling Xu, Chenjie Su, and Zengping Zhang
- Published
- 2021
43. Long-Term Quality of Life in Adult Patients with Strabismus after Corrective Surgery Compared to the General Population.
- Author
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Meiping Xu, Huanyun Yu, Yuanyuan Chen, Jinling Xu, Jingwei Zheng, and Xinping Yu
- Subjects
Medicine ,Science - Abstract
To evaluate the status of and factors associated with long-term health-related quality of life (HRQOL) in adult patients with strabismus following corrective surgery.Prospective cross-sectional study. A total of 122 adults who underwent corrective surgery and were followed up for at least 1 year were recruited. Pre- and post-operative HRQOL were evaluated using the Chinese version of the Adult Strabismus 20 (AS-20). Demographics and clinical characteristics were recorded. Eighty-nine age-matched, normal individuals without a history of strabismus were recruited as a control group.AS-20 scores improved significantly in the psychosocial subscale and total scale after surgery for all122 patients, but not in the function subscale. HRQOL was better in the successful cases than in the non-successful cases (p
- Published
- 2016
- Full Text
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44. Exotropia Is the Main Pattern of Childhood Strabismus Surgery in the South of China: A Six-Year Clinical Review
- Author
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Xinping Yu, Zhouduo Ji, Huanyun Yu, Meiping Xu, and Jinling Xu
- Subjects
Ophthalmology ,RE1-994 - Abstract
Purpose. To evaluate the distribution pattern and changes of strabismus surgery in children based on the data collected from a local eye hospital in the south of China between 2006 and 2011. Methods. A retrospective analysis of all strabismus surgeries in children (
- Published
- 2016
- Full Text
- View/download PDF
45. Health-related quality of life correlated with the clinical severity of intermittent exotropia in children
- Author
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Yuanyuan Wang, Fang Hou, Jiawei Zhou, Xinping Yu, Jinling Xu, Huanyun Yu, and Meiping Xu
- Subjects
Health related quality of life ,Pediatrics ,medicine.medical_specialty ,genetic structures ,business.industry ,medicine.disease ,eye diseases ,humanities ,03 medical and health sciences ,Ophthalmology ,0302 clinical medicine ,Poor control ,030221 ophthalmology & optometry ,Medicine ,Multiple linear regression analysis ,Clinical severity ,Sensory fusion ,business ,Strabismus ,Intermittent exotropia ,Exotropia ,030217 neurology & neurosurgery - Abstract
To evaluate whether the results of the health-related quality of life (HRQOL) is associated with the various clinical aspects of intermittent exotropia in children. The HRQOL of children and their parents was evaluated prospectively using the Intermittent Exotropia Questionnaire (IXTQ). The deviation angle, stereo function, sensory fusion, and strabismus control were measured. Two hundred and sixty six children with intermittent exotropia (aged 5–17 years) were included in the study. Child HRQOL was significantly correlated with clinical severity; lower IXTQ scores were associated with a larger deviation (p
- Published
- 2019
46. miR-142-3p as a novel biomarker for predicting poor prognosis in renal cell carcinoma patients after surgery
- Author
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Fangting Zhang, Xiqi Peng, Weijie Xu, Yongqing Lai, Xiang Pan, Liwen Zhao, Jinling Xu, Hang Li, Xin Guan, Kaihao Liu, and Chunduo Zhang
- Subjects
Male ,0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Poor prognosis ,Clinical Biochemistry ,Total rna ,medicine.disease_cause ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Renal cell carcinoma ,Internal medicine ,microRNA ,Biomarkers, Tumor ,medicine ,Humans ,Prognostic biomarker ,Carcinoma, Renal Cell ,business.industry ,Middle Aged ,Prognosis ,medicine.disease ,Kidney Neoplasms ,MicroRNAs ,030104 developmental biology ,Mir 142 3p ,030220 oncology & carcinogenesis ,Biomarker (medicine) ,Female ,business ,Carcinogenesis - Abstract
Background: miR-142-3p has proved to be involved in tumorigenesis and the development of renal cell carcinoma. The present study aimed to explore the prognostic value of miR-142-3p. Methods: Total RNA was extracted from renal cell carcinoma specimens and the expression level of miR-142-3p was measured. Pearson Chi-square test, Kaplan–Meier analysis, as well as univariate and multivariate regression analysis were performed to determine the correlation between miR-142-3p and the prognosis of renal cell carcinoma patients. Receiver operating characteristic curves were constructed to evaluate the predictive efficiency of miR-142-3p for the prognosis of renal cell carcinoma patients. Data from The Cancer Genome Atlas (TCGA) were utilized to validate our findings. Results: Our results demonstrated that upregulation of miR-142-3p was correlated with shorter overall survival (P=0.002) and was, in the meantime, an independent prognostic factor for renal cell carcinoma patients (P=0.002). The receiver operating characteristic curve combining miR-142-3p expression with tumor stage showed an area under the curve of 0.633 (95% confidence interval 0.563, 0.702). The result of TCGA data was consistent with our findings. Conclusions: Our results suggest miR-142-3p expression is correlated with poor prognosis of renal cell carcinoma patients and may serve as a prognostic biomarker in the future.
- Published
- 2019
47. A Top-N recommendation algorithm based on graph convolutional network that integrates basic user information
- Author
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XiaoDong Cheng, ZengPing Zhang, Ting Wang, JinLing Xu, and ChenJie Su
- Subjects
User information ,Small-world network ,Cold start ,Computer science ,Collaborative filtering ,Graph (abstract data type) ,Relevance (information retrieval) ,Algorithm ,MovieLens ,Network model - Abstract
In order to solve the problem of data sparseness and cold start of the collaborative filtering model, many methods have been proposed, but most of them ignore the user attribute similarity and the user preference. The accuracy of recommendation needs to be improved. Most of researches stay in simple linear modeling of the relationship between users and items, and does not consider the influence of auxiliary information on the recommendation algorithm. In our real life, users preferences are affected by age, gender, and personality. Environment, social circle, etc.In this work, we design a Top-N recommendation algorithm LNGCF-B (light neural graph collaborative filtering with user basic information). Firstly, different from traditional graph convolutional collaborative filtering algorithm, the simplified version is more explanatory, the training time is shortened. Secondly, this algorithm considers the attributes of the user, experiments show that LNGCF-B is better than the baseline algorithm. In our social life, there are many different types of networks, under different network models, the performance of the recommendation algorithm is also different. However, there are few researches on the performance of recommendation algorithms in different scenarios. We use LNGCF-B on two data sets belonging to different network models. The results show that the list recommended by the algorithm on the Movielens 100K data set belonging to the scale-free network has a higher degree of relevance, and the Facebook friend relationship data set belonging to the small world network has a higher recall rate.
- Published
- 2021
48. M378 exhibits anti-inflammatory activities through NLRP3 signaling pathway
- Author
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Jinling Xu, Qi Lv, Shumin Pan, Huanhuan Qiu, Yu Liao, Ming Zhou, Weijie Li, Caiyan Li, Pan Zhang, Yujian Li, Guanglin Xu, and Qingfeng Yu
- Subjects
Lipopolysaccharides ,Pharmacology ,Inflammasomes ,Anti-Inflammatory Agents, Non-Steroidal ,Caspase 1 ,Interleukin-1beta ,Anti-Inflammatory Agents ,Mice, Inbred C57BL ,Mice ,NLR Family, Pyrin Domain-Containing 3 Protein ,Animals ,Hydrogen Sulfide ,Reactive Oxygen Species ,Signal Transduction - Abstract
Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used drugs due to their values in attenuating pain, fever and inflammation. Unfortunately, conspicuous adverse effects, such as gastrointestinal (GI) damage and/or cardiovascular events have impeded their application in clinic. M378 is a novel hydrogen sulfide-releasing NSAIDs with uncompromised potency and negligible toxicity compared to the existing NSAIDs. However, its anti-inflammatory activity and mechanism are still an enigma. Here we investigated the effect of M378 on the NLRP3 inflammasome signaling pathway and addressed the underlying molecular mechanism. Our data in vitro showed that M378 dose-dependently inhibited the cleavage of Caspase-1 and the secretion of active IL-1β and blocked NLRP3-dependent pyroptosis in LPS-primed J774A.1 macrophages. Furthermore, M378 remarkably inhibited upstream ASC oligomerization and ROS production regarding the process of NLRP3 inflammasome assembly. Our data in vivo demonstrated that M378 protected mice from acute liver injury, reducing the levels of ALT/AST and IL-1β and improving hepatic pathological damages. Immunoblot analysis revealed that M378 inhibited the expressions of Caspase-1 and IL-1β in liver tissues of ALI mice. We also showed that M378 alleviated IL-1β secretion and peritoneal neutrophils infiltration in MSU-elicited acute peritonitis mice. In conclusion, M378 exerted its anti-inflammatory effect both in vitro and in vivo and its mechanisms are at least connected to its inhibitory performance on the generation of ASC oligomers and ROS production. These findings give an insight. into the molecular mechanism of hydrogen sulfide-releasing NSAIDs and support a potent therapeutic role of M378 in the treatment of NLRP3-driven inflammatory diseases.
- Published
- 2022
49. Neuregulin 4 Attenuates Osteoarthritis Progression by Inhibiting Inflammation and Apoptosis of Chondrocytes in Mice
- Author
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Jinling Xu, Guosheng Gan, Jiayue Tong, Yangyang Cheng, Yin Sun, Kaiyue He, Biying Meng, Xiaoli Xu, Lingfeng Shi, and Guangda Xiang
- Subjects
MAPK/ERK pathway ,Cartilage, Articular ,Endocrinology, Diabetes and Metabolism ,Type II collagen ,Inflammation ,Apoptosis ,Chondrocyte ,Mice ,Endocrinology ,Chondrocytes ,Osteoarthritis ,Medicine ,Animals ,Orthopedics and Sports Medicine ,education ,Protein kinase A ,Cells, Cultured ,Neuregulins ,Neuregulin-4 ,education.field_of_study ,business.industry ,Kinase ,medicine.anatomical_structure ,Cancer research ,Disease Progression ,Signal transduction ,medicine.symptom ,business - Abstract
Osteoarthritis (OA) is characterized by chondrocyte apoptosis and increased degradation of type II collagen. Inflammation is one of the major risk factors involved in the pathophysiology of OA. Neuregulin 4 (Nrg4) plays a protective role in a variety of low-level inflammatory diseases, such as non-alcoholic fatty liver disease, inflammatory bowel disease, or type 2 diabetes mellitus. Here we found that (1) Nrg4 deficiency aggravated the destruction and inflammation of articular cartilage and the apoptosis of chondrocytes in vivo. (2) Nrg4 restoration reversed these changes in vivo. (3) Murine recombinant Nrg4 (rNrg4) suppressed inflammation and apoptosis of chondrocytes and decreased the degradation of extracellular matrix in vitro. (4) Mechanistically, the mitogen-activated protein kinase/c-jun N-terminal kinase (MAPK/JNK) signaling pathway may be involved in the regulation of Nrg4 in the pathophysiology of OA. Therefore, we concluded that Nrg4 alleviated the progression of OA by inhibiting the inflammation, protecting against apoptosis of chondrocyte, and decreasing the degradation of extracellular matrix in a manner involving MAPK/JNK signaling.
- Published
- 2021
50. Historical trends in atmospheric metal(loid) contamination in North China over the past half-millennium reconstructed from subalpine lake sediment
- Author
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Mengyao Liang, Enfeng Liu, Xiaoyu Wang, Qinghui Zhang, Jinling Xu, Ming Ji, and Enlou Zhang
- Subjects
China ,Geologic Sediments ,Health, Toxicology and Mutagenesis ,Mercury ,General Medicine ,Toxicology ,Pollution ,Lakes ,Coal ,Lead ,Metals, Heavy ,Water Pollutants, Chemical ,Cadmium ,Environmental Monitoring - Abstract
Trace metal (loid) contamination in the atmosphere is widely monitored, but there is a gap in understanding its long-term patterns, especially in North China, which is currently a global contamination hotspot mainly caused by heavy industry emissions and coal combustion. Herein, historical trends of atmospheric As, Cd, Cr, Cu, Hg, Ni, Pb and Zn contamination in North China over the past ∼500 years are comparatively studied with sediment cores from two subalpine lakes (Gonghai and Muhai). Arsenic, Pb, Cd and Hg were main pollutants according to Pb isotopes and enrichment factors. Mercury contamination has increased continuously since the late 1800s and increasing As, Pb and Cd contamination started in the 1950s in Gonghai. In contrast, the contamination in Muhai lagged two decades for As, Cd and Pb and a half-century for Hg behind that in Gonghai, although the trends were similar. This contamination lag was attributed to the low sensitivity of Muhai sediment to early weak atmospheric metal contamination under 2.1-fold higher detrital sedimentation. As, Pb and Cd contamination has intensified since the 1980s, and the metals showed similar sedimentary fluxes in the cores. However, sedimentary fluxes of Hg contamination were 3.4-fold higher in Gonghai than in Muhai due to combination with organic matter. No obvious Cr, Cu and Ni contamination in the cores was mainly because of the low atmospheric deposition from anthropogenic sources relative to detrital input, although some of their atmospheric emissions were higher than those of As, Cd and Hg. Atmospheric As, Pb and Cd contamination was mainly from domestic sources of coal combustion and nonferrous smelting. Mercury contamination was mainly from global and Asian sources in the first half of the 20th century, and domestic emissions gradually dominated Hg contamination after the mid-1900s.
- Published
- 2022
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