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Backgrounds: A proper disinfection of denture is vital to prevent a fungal infection. A study on the feasibility of microencapsulated phytochemical as complementary disinfectant and its interaction with effervescent tablet immersion on denture base resin is lacking. Objectives: The aim of this study was to examine the feasibility of phytochemical-filled microcapsules as disinfectant for the inhibition of Candida albicans (C. albicans) attachment on the denture base produced by digital light processing (DLP). Methods: 54 denture base specimens uniformly mixed with or without 5wt% phytochemical-filled microcapsules were prepared using DLP. Fungal cells were inoculated onto the surfaces of the specimens, which were divided into three different disinfection treatment groups (n = 9): 1) none, 2) sterile tap water immersion for 15 min, and 3) effervescent tablet immersion for 15 min. After each treatment, the biofilm on denture surface was stained with a crystal violet solution to measure the absorbance. The number of fungal colonies was counted as colony-forming units (CFU) per mL. Morphological changes were examined by microscopy. An aligned rank transform analysis of variance was performed to analyze the interaction of presence of microcapsule and disinfection condition, with statistical significance set at P < 0.05. Results: Both for the absorbance and CFU, there was no significant interaction between the presence of microcapsules and disinfection conditions (P = 0.543 and P = 0.077, respectively). The presence of microcapsules was statistically significant (both P < 0.001), while the effect of disinfection condition was not significant (P = 0.165 and P = 0.189, respectively). Morphological changes in fungi were detected in the groups containing microcapsules, whereas undamaged hyphal structures were found in those without microcapsules, irrespective of disinfection treatments. Conclusions: The presence of phytochemical-filled microcapsules significantly reduced the adhesion of C. albicans and inhibited its proliferation on denture surfaces, regardless of disinfection conditions. [ABSTRACT FROM AUTHOR]

Dental implants are inevitably exposed to bacteria in oral cavity. Understanding the colonization of bacteria on implant surface is necessary to prevent bacteria-related inflammation surrounding dental implants. The purpose of this study was to investigate the effect of surface properties on biofilm formation on the implant surface. One early colonizer, Streptococcus gordonii (S. gordonii), and two late colonizers, Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis), were grown on the titanium and zirconia surfaces with two types of surface roughness for 24 and 72 h. Each bacterial biofilm on specimens was quantified using crystal violet assay and observed by scanning electron microscopy. S. gordonii formed more biofilm on the titanium surface than zirconia at the same roughness and more biofilm on the rough surface than smooth one of the same materials at 24 and 72 h of incubation. F. nucleatum adhered on all the surfaces at 24 h and proliferated actively on the surfaces except smooth zirconia at 72 h. P. gingivalis proliferated vigorously on the surfaces at 72 h while it scarcely adhered at 24 h. There was no consistent correlation between contact angle and biofilm formation of the three bacteria. The three bacteria proliferated most on the rough titanium surface and least on the smooth zirconia surface. In addition, the proliferation was affected by the bacterial species as well as the surface properties. [ABSTRACT FROM AUTHOR]