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This is the twelfth of a series of miscellaneous contributions, by various authors, where hitherto unpublished data relevant to both the Med-Checklist and the Euro+Med (or Sisyphus) projects are presented. This instalment deals with the families Asparagaceae (incl. Hyacinthaceae), Boraginaceae, Cactaceae, Caryophyllaceae, Chenopodiaceae, Compositae, Crassulaceae, Euphorbiaceae, Gramineae, Haloragaceae, Iridaceae, Labiatae, Leguminosae, Malvaceae, Orchidaceae, Orobanchaceae, Plumbaginaceae, Polygonaceae, Rosaceae, Scrophulariaceae (incl. Buddlejaceae), Solanaceae and Umbelliferae. It includes new country and area records and taxonomic and distributional considerations for taxa in Abutilon, Aegilops, Amelanchier, Andryala, Aruncus, Asparagus, Bellevalia, Brugmansia, Buglossoides, Bupleurum, Cortaderia, Crassula, Datura, Dysphania, Euphorbia, Fallopia, Iris, Lycianthes, Myriophyllum, Nicodemia, Onobrychis, Ophrys, Opuntia, Orobanche, Phelipanche, Plumbago, Salvia, Silene, Stellaria and Wisteria, and new combinations in Amelanchier and Phelipanche. © 2020 Institute of Electrical and Electronics Engineers Inc.. All rights reserved.

The vegetation of thermophilic trampled habitats was sampled in the Bay of Kvarner according to the standard procedures of the Braun- Blanquet method. The following associations were determined: Polycarpo-Amaranthetum deflexi Pignatti 1953, Euphorbio-Oxalidetum corniculatae Lorenzoni 1964, Euphorbietum maculatae Poldini 1989, Eleusinetum indicae Pignatti 1953. Among them a new association was described: Catapodio loliacei-Herniarietum rotundifoliae ass. nova. The Euphorbia prostrata community, Trifolium repens community, and the Cynodon dactylon community were not assigned to the association. The ordination diagram (Princoor program) shows the main differences among the communities. On the first axis, there is a gradient from the most thermophilic Cynodontetum dactylon comm. and Catapodio-Herniarietum to the more mesophilous and less trampled Polycarpo-Amaranthetum and Cynodon dactylon community to all other communities with more Eragrostietalia elements.

This dataset provides the Global Naturalized Alien Flora (GloNAF) database, ver-sion 1.2. Glo NAF represents a data compendium on th e occurrence and identit y of naturalizedalien vascular plant taxa across geographic regions (e.g. countries, states, provinces, districts,islands) around the globe. The dataset includes 13,939 taxa and covers 1,029 regions (including381 islands). The dataset is based on 210 data sources. For each ta x on-b y-region combination, wepr ovide information on whether the tax on is consider ed to be naturalized in the specific region(i.e. has established self-sustaining popula tions in the wild). Non-native taxa are marked as“alien”, when it is not clear whether they are naturalized. To facilitate alignment with other plantdatabases, we pro v ide f or each taxon the name as given in the original data source and the stan-dardized taxon and family names used by The Plant List Version 1.1 (http://www.theplantlist.org/). We pro vide an ESRI shapefile including polygons f or each region and informa tion on whetherit is an island or a mainland region, the country and the Taxonomic Databases Working Group(TDWG) regions it is part of (TDWG levels 1–4). We also provide several variables that can beused to filter the data according to quality and completeness of alien taxon lists, which varyamong the combinations of regions and da ta sources. A pre vious version of the GloNAF dataset(version 1.1) has already been used in several studies on, for example, historical spatial flows oftaxa between continents and geographical patterns and determinants of naturalization across dif-ferent taxonomic groups. We intend the updated and expanded GloNAF version presented hereto be a global resource useful for studying plant inv asions and changes in biodiversity from regio-nal to global scales. We release these data into the public domain under a Crea ti ve CommonsZer o license waiver (https://creati v ecommons.org/share-y our -work/public-domain/cc0/). Wheny ou use the da ta in your publication, we request that y ou cite this da ta paper. If GloN AF is amajor part of the data analyzed in your study, you should consider inviting the GloNAF coreteam (see Metadata S1: Originators in the Overall project description) as collaborators. If youplan to use the GloNAF dataset, we encourage y ou to contact the GloNAF core team to checkwhether there have been recent updates of the dataset, and whether similar analyses are already ongoing.

Moltkia petraea (Tratt.) Griseb. (Boraginaceae) is an endemic, lithophytic, xerothermic Illyric–Balkan species distributed along the Adriatic Coast in Croatia, Bosnia and Herzegovina, Montenegro, Albania, and Greece, mostly in Mediterranean and subMediterranean regions. It is very interesting species from the horticultural point of view which possesses the notable antioxidant activity. But, the data about chemical constituents of this species are very limited. The aim of this study was to investigate content of phenolic compounds using HPLC in ethanol extracts of leaves, flowers and stems of M. petraea from two mountainous localities (Mt Biokovo and Mt Sniježnica) in Croatia. Seven phenolic compounds (naringenin, quercetin, rutin, and caffeic, ferulic, rosmarinic, and sinapic acid)were identified and quantified. Among them naringenin and rosmarinic acid were identified in all extracts. On the other hand, quercetin (0.006%) was found only in leaves from Mt Biokovo, while ferulic acid (0.007%) was identified only in stems from the same locality. The most abundant compound was naringenin and its concentration ranged from 1.473% in stems from Mt Sniježnica to 4.241% in flowers from Mt Biokovo. The obtained results present worth additional knowledge on the genus Moltkia Lehm. in general, and particularly on the endemic M. petraea.

Geranium dalmaticum (Beck) Rech. f. (family Geraniaceae) is an endemic Illyric-Balcanic species which grows at the edges of karst valleys and cracks, and in crevices of calcareous rocks in Croatia Montenegro and Albania at altitudes from 200 m to 961 m. It is a perennial herb up to 15 cm high with dull purplish-red flowers. On the other hand, G. macrorrhizum L. is a perennial herb up to 50 cm high with larger leaves and dull purplish-red flowers. G. macrorrhizum grows in mountainous and shady places on calcareous soils in France, Italy, Austria, Balkan Peninsula, Romania, and west Russia. The aim of this study was to get insight in content of phenolic compounds using HPLC in ethanol extracts of above ground part of G. dalmaticum from Mt Sv. Ilija (Croatia), and G. macrorrhizum from Mt Velebit (Croatia). Only quercetin (0.230%) was identified in G. dalmaticum herba, while only rutin (1.116%) was found in G. macrorrhizum. On the other hand, the naringenin, and caffeic, coumaric, ferulic, rosmarinic, and sinapic acid were not identified in investigated species. The obtained results present worth additional knowledge on the genus Geranium L. in general, and particularly on the endemic G. dalmaticum.

Blue-green algae or cyanobacteria are photosynthetic microorganisms that can be found in most habitats and have ability to multiply quickly (forming “blooms”) under specific conditions. Nitrogen and phosphorus enriched water in combination with higher temperature, lower water level and anthropogenic pressure enhances effects of algal blooms. Surveys in different parts of the world have found that approximately 45% to 90% of cyanobacterial blooms produce toxins (cyanotoxins). Reservoirs and slow moving rivers can provide suitable conditions for cyanobacterial bloom development, which may decrease water quality and affect various biological communities. Investigation of cyanobacterial diversity and composition was conducted along the karstic river Cetina and artificial reservoir Peruča which is used for recreational purposes, in the sub mountain Croatia. Samples were collected in August 2012 along the river (~ 32, 5 river kilometers) on 23 study sites including spring and main flow of the river Cetina (upstream and downstream from the reservoir Peruča). A total of 22 taxa were found of which Anabaena sp., Chroococcus sp., Gleocapsa sp., Merismopedia sp., Nostoc sp., Oscillatoria sp. and Pseudanabaena sp. were dominant. Moreover, five taxa with toxic properties: Anabaena sp., Lyngbya sp., Microcystis sp., Nostoc sp. and Oscillatoria sp. have been recorded. Generated taxonomical list of cyanobacteria provided insight information on biodiversity of the river Cetina and reservoir Peruča. Obtained data showed diversity in algological flora as a result of dynamics in environmental conditions. Physical parameters which were recorded along the research sites showed that the pH of the river and the reservoir were similar and ranged from 5, 6 to 6, 3 units what corresponded to the ecological status of the observed water system due to external factors, physical parameters and a large amount of degraded organic matter with dominance of cyanobacterial taxa in the water column. Furthermore, research on the river Cetina indicates how this type of aquatic habitat operates and it can point out how some similar ecosystems can function. Long-term climate change predictions suggest that changes in temperature and weather patterns may increase the scale of hazard presented by cyanobacterial blooms. This in turn may increase the scale of risk to human health by cyanotoxins. Planning and preparedness with respect to cyanobacterial investigations will therefore remain important for the foreseeable future.

Chlorophylls and associated carotenoid pigments are being used to map the chemotaxonomic composition of phytoplankton in the oceans. Compared with larger eukaryotic cells, photosynthetic picoplankton possesses better nutrient uptake capabilities which result in its domninace and higher level of importance with increased oligotrophy. In order to determine spatial distribution of phytoplankton pigments in olygotrophic environment in winter conditions an oceanographic cruise was conducted in southern Adriatic from February 28th till March 3rd 2015. Two major transects were investigated: (i) Dubrovnik to 1000m isobath, and (ii) 1000m isobath to Lastovo island. Total of 45 CTD casts was performed in order to compare bio-optical measurements of chlorophyll fluorescence (Chl F) with pigment concentration. A total of 117 samples were collected on the basis of CTD profile and fluoresces signal and analysed by high- performance liquid chromatography (HPLC). Pigment concentration was extremely low (chlorophyll a maximum concentration was 190ngL-1) reflecting oligotrophy of the environment. Fluorescence signal was detected in depths up to 450m, which was also confirmed by HPLC pigment analyses of Chl a. Beside Chl a, most frequently detected and in highest concentrations were 19’ hexanoyloxyfucoxanthin, fucoxantin, 19’ butanoyloxyfucoxanthin, zeaxantin and one unidentified pigment. All pigments showed high patchiness in their spatial distribution. The unidentified pigment was detected at 500m depth, i.e. in the layer where Chl a was not detected any more. Its identification is the following step in the research.

Carob seeds (Ceratonia siliqua L., Fabaceae) are very important source of galactomannan oligosaccharide. However, it was believed that the weight of individual carob seed is constant and weighs about 200 mg each. So, that was the reason why the carob seeds were used as a measure for carat in the past. Because of hardness of carob seeds and consequently specific demands for their processing, numerous researches of their morphological and micromorphological characteristics were carried out. Within research project TEUCLIC(“Taxonomy ecology and utilization of carob tree and bay laurel in Croatia, financed by Croatian Foundation for Science) the total of 1309 individual carob seeds, separated from the carob pods collected from different carob trees on the Island of Drvenik Mali, were investigated during the first research year. The average weight of individual seed is 191 mg(SD=0.044, SEM=0.0012). Our results of micro morphological studies showed the very high share of mechanical tissue both in pods and seeds. However, achieved results of morphological and micro morphological characteristics of carob seeds correspond with the results of previous researchers who investigated the seed morphology of carob tree populations in Mediterranean countries.