Search

Tropical forests are threatened by anthropogenic activities such as conversion into agricultural land, logging and fires. Land-use change and disturbance affect ecosystems not only aboveground, but also belowground including the ecosystems' carbon and nitrogen cycle. We studied the impact of different types of land-use change (intensive and traditional agroforestry, logging) and disturbance by fire on fine root biomass, dynamics, morphology, and related C and N fluxes to the soil via fine root litter across different ecosystems at different elevational zones at Mt. Kilimanjaro (Tanzania). We found a decrease in fine root biomass (80–90%), production (50%), and C and N fluxes to the soil via fine root litter (60–80%) at all elevation zones. The traditional agroforestry 'Chagga homegardens' (lower montane zone) showed enhanced fine root turnover rates, higher values of acquisitive root morphological traits, but similar stand fine root production, C and N fluxes compared to the natural forest. The decrease of C and N fluxes with forest disturbance was particularly strong at the upper montane zone (60 and 80% decrease, respectively), where several patches of Podocarpus forest had been disturbed by fire in the previous years. We conclude that changes on species composition, stand structure and land management practices resulting from land-use change and disturbance have a strong impact on the fine root system, modifying fine root biomass, production and the C and N supply to the soil from fine root litter, which strongly affects the ecosystems' C and N cycle in those East African tropical forest ecosystems.

The capability of coastal wetland soils to store large amounts of organic carbon (OC) has been increasingly recognised. Stabilisation mechanisms (e.g. aggregation or mineral association) and stability of organic matter (OM) (recalcitrant vs. labile) are important features for the long-term storage of soil organic carbon (SOC). In estuarine marshes, SOC storage is dominated by a complex and dynamic interaction of abiotic conditions such as tidal inundation or changes in salinity. However, little is known on OC stabilisation and stability in these transitional ecosystems and how they are affected by system-specific characteristics. Therefore, our aim was to assess the effect of flooding and salinity on (i) OC stabilisation by aggregation and mineral association and (ii) the stability of the OC pool in estuarine marsh soils.We analysed topsoil (0 – 10 cm) and subsoil (10 – 30 cm) samples from 9 marsh zones along the salinity gradient (salt, brackish and freshwater) and flooding gradient (pioneer zone, low and high marsh) of the Elbe Estuary for their SOC contents, OC stabilisation mechanisms (density fractionation), OC stability (incubation with one- and two-compartment model fits) and dissolved organic carbon (DOC) concentrations.Total SOC contents were highest in the freshwater marsh and decreased towards topsoils with higher salinity. Flooding frequency had no uniform effect on SOC contents: While there was a positive tendency with decreasing flooding frequency, subsoils of the freshwater marsh showed the opposite trend. Total SOC contents were positively correlated with mineral-associated OC (CMAOM) and pedogenically unprotected particulate OM (CfPOM). The highest proportion of CMAOM was found in topsoils of freshwater marshes and it decreased towards higher salinities in topsoils of high marshes and pioneer zones. The OM protection by aggregation (CoPOM) increased in topsoils of high marshes. The proportion of CfPOM was less directly affected by salinity and flooding than by the CN ratio of the aboveground biomass (CNlitter). Furthermore, CfPOM correlated positively with the potential mineralisable C (Cpot) and labile C (Clabile) and negatively with the recalcitrant C pool (Crecalcitrant) that were derived from the one- and two-compartment models. Labile C, Cpot and Crecalcitrant were also strongly influenced by CNlitter. Moreover, Crecalcitrant was linked to the proportion of CMAOM. Concentrations of DOC increased with total SOC and Cpot but decreased with CoPOM.We conclude that SOC stabilisation in the Elbe Estuary is mainly related to mineral association of OM. With increasing terrestrial influence, physical protection in aggregates becomes more important. Besides these pedogenic stabilisation mechanisms, recalcitrance is strongly determined by vegetation characteristics.

Trees in forests and urban environments are increasingly under pressure due to extended periods of drought in central Europe. At the same time, their transpiration performance is all the more important to counteract drought and heat. Defining drought itself, as well as trees’ physiological response strategies, can be closely linked to soil hydrological conditions. In a three-year field experiment with young trees of different species planted in different substrates (n = 3 substrates * 9 species * 5 repetitions = 135 trees) in Northern Germany, we found that soil hydraulic properties strongly affected tree vitality, and that the species’ reactions towards unfavorable conditions differed significantly. This might be due to species-specific transpiration regulation strategies under drought stress. In a second step, we now link tree diameter fluctuations, which have been shown to closely correlate with transpiration, to soil water conditions. To this end, subsets of trees were equipped with dendrometers – 15 trees of one species (Quercus cerris) in three different substrates (sand, planting substrate, and loamy silt) in the growing season of 2020, and 21 trees of seven different species (Amelanchier lamarkii, Carpinus betulus ‘Lucas’, Geleditsia traconathos ‘Skyline’, Liquidambar styraciflua, Ostrya carpinifolia, Quercus palustris, Tilia cordata ‘Greenspire’) in sand substrate in the growing season of 2021. The dendrometer measurements were partly combined with soil water tension measurements (2020: n=6, 2021: n=10). By comparing statistical characteristics of the time series, we want to (1) link soil water and transpiration dynamics and (2) differentiate this link for different substrates and species. Such, tree stem diameter fluctuations, coupling soil conditions and physiological properties, might provide insight into the effect of tree strategic responses to soil drought.

Trees as essential components of green urban structures are of crucial importance for the regulation of the urban climate and human wellbeing. Despite this, the currently rising demand for living space and infrastructure causes an increase in the share of sealed and compacted soils. These trends directly affect soil-plant interactions in urban environments. The synergy of the increasing land use pressure and changing climatic conditions worsen the site and growth conditions and thus the vitality for young and mature trees. A possible adaptation strategy is the transformation of plant pits into water reservoirs combining the discharge of excess water with impermeable sole materials and substrates that optimise the water conductivity and storage capacity. The corresponding aim of this study is the quantification of the effects of the water balance dynamic in the rooting zone on the vitality of young trees at highly sealed sites in the city of Hamburg. The two main questions are 1) Do technically modified plant pits reduce summerly drought stress inside the rooting zone and thus improve the root water uptake and tree vitality?, and 2) Does excess water after high rainfall limit the gas exchange and thus the root growth? To answer these questions, we selected two different sites, one residential area and one pedestrian zone. A total of 13 tree planting pits, including 5 technically modified and 8 generally constructed ones, with two types of substrates and water discharge, are equipped with TDR- and water tension sensors for a continuous monitoring of the soil water balance and O2 and CO2 sensors and tubes for monitoring the gas household. Stomatal resistance, chlorophyll content and fluorescence as well as Δ13C isotope measurements are combined with branch and trunk growth measurements and a tree appraisal to investigate the tree vitality. The comparative data analysis will be used for evaluating the different planting pit variants to give development as well as dimensioning recommendations for prospective planting pit constructions, improving the soil-plant interaction.

Climate change increases the pressure on urban street trees by limited soil-water availability during extended heat and dry summer periods. Young and freshly planted trees are particularly affected by soil drought since their root system is not well developed and spatially limited to the volume of the initial root ball. The vitality and survival of these trees is strongly dependent on their ability to quickly exploit a larger rooting zone.To investigate early tree development and root growth, we established a field trial in a tree nursery within the metropolitan region of Hamburg, Germany. Three tree species (Amelanchier lamarckii, Quercus cerris and Tilia cordata ‚Greenspire‘) were grown in two soil substrates (loam, sand) in five replicates. After three years, we excavated a defined soil volume radially from each tree trunk, and determined root biomass (coarse > 2 mm, and fine < 2 mm diameter) in three distances and three depths. Results were compared to species-specific allometric equations, derived from stem diameter measurements.While no overall substrate effect appeared for total root biomass, the average fine-root biomass between all species was 59% higher in loam compared to sand. Species wise, A. lamarckii showed 68% lower total root biomass and a lower root spread in sand substrate, compared to loam. This was mainly related to the near complete absence of A. lamarckii‘s coarse roots in sand. In contrast Q. cerris developed larger root biomass in sand substrate, particularly in form of deep fine roots, with a maximum in 60-90 cm depth. This was not reflected in the allometric equation (r = -0.8), indicating a shift in belowground carbon allocation under water stress. Compared to the restricted root system of A. lamarckii and the deep roots of Q. cerris, T. cordata formed an extensive fine root system, with a reduced fine root abundance in sand substrate.We conclude that the rooting-zone exploitation in planting pits is strongly affected by soil substrate and differs between tree species. Particularly Q. cerris invests in a large deep rooting system under enhanced water stress (i.e. in sand substrate), which is not reflected by common allometric equation methods. Ensuring a healthy urban tree population under climate change, therefore requires the combined consideration of adaptive tree species and present urban soil substrates for new tree plantings.

Aims Inoculation with climate-adapted rhizobia is able to increase legume productivity in drought-prone regions of Sub-Saharan Africa. Enhanced nodulation might additionally affect plant-soil interactions and control rhizosphere carbon (C) and nitrogen (N) pools. Methods We investigated inoculation effects on nodulation and biological N2 fixation (BNF) of Vigna unguiculata and consequent effects on C and N pools in two Namibian soils. Three treatments (Bradyrhizobium sp.1–7 inoculant, non-inoculated, N-fertilised with 50 kg N ha−1) were applied in rhizoboxes at 45% and 20% maximum water holding capacity. Nodule development was photo-documented, and rhizobia-DNA sequences were identified. BNF was assessed by δ15N enrichment, and organic C and N pools were analysed in bulk and root adherent soil. Results Plant growth initially enhanced mineral N losses from the rhizosphere at flowering stage (6 weeks growth), but led to a re-increase of N, and organic C contents after ripening (10 weeks). Inoculation had no effect on nodulation and soil C and N pools, indicating that both soils contained sufficient indigenous rhizobia to allow effective nodulation. However, the inoculant strain was more competitive in establishing itself in the root nodules, depending on the local conditions, showing a need for regional adjustment of inoculation strategies. Conclusion Water stress was the main limitation for nodulation and, in combination with soil type, substantially affected rhizosphere and bulk soil C and N contents. The temporally enhanced rhizodeposition after ripening could be able to maintain soil C and N pools after legume cultivation.

Tropical forests represent the largest store of terrestrial biomass carbon (C) on earth and contribute over-proportionally to global terrestrial net primary productivity (NPP). How climate change is affecting NPP and C allocation to tree components in forests is not well understood. This is true for tropical forests, but particularly for African tropical forests. Studying forest ecosystems along elevation and related temperature and moisture gradients is one possible approach to address this question. However, the inclusion of belowground productivity data in such studies is scarce. On Mt. Kilimanjaro (Tanzania), we studied aboveground (wood increment, litter fall) and belowground (fine and coarse root) NPP along three elevation transects (c. 1800–3900 m a.s.l.) across four tropical montane forest types to derive C allocation to the major tree components. Total NPP declined continuously with elevation from 8.5 to 2.8 Mg C ha−1 year−1 due to significant decline in aboveground NPP, while fine root productivity (sequential coring approach) remained unvaried with around 2 Mg C ha−1 year−1, indicating a marked shift in C allocation to belowground components with elevation. The C and N fluxes to the soil via root litter were far more important than leaf litter inputs in the subalpine Erica forest. Thus, the shift of C allocation to belowground organs with elevation at Mt. Kilimanjaro and other tropical forests suggests increasing nitrogen limitation of aboveground tree growth at higher elevations. Our results show that studying fine root productivity is crucial to understand climate effects on the carbon cycle in tropical forests.

Tropical mountain ecosystems cover a broad variety of climatic and vegetation zones and are global hotspots of biodiversity. These ecosystems are severely threatened by climate and land-use change, which also strongly affect soil properties. Mt. Kilimanjaro, with its large elevation gradient and relatively homogeneous geology of volcanic rocks and ashes, provides a unique opportunity to study and interpret soil organic matter (SOM) responses to climatic changes. Our objectives were to identify key SOM compounds in six elevation zones (covering ecosystems from tropical dry-lowland, through montane forest, up to alpine heathlands) that are affected by the climatic changes along a 3400 m transect, and to relate these SOM changes to ecosystem specific characteristics. The SOM composition in four topsoils (0–10 cm) samples per ecosystem was characterized by analytical double-shot pyrolysis-gas chromatography–mass spectrometry (Py-GC/MS). Evolving-gas analysis-mass spectrometry (EGA-MS) was used to quantitatively compare the thermal desorption (first shot: The percentage of thermally desorbed compounds increased 10 fold from lowland ( 2000 m), followed by a 40% decrease in alpine ecosystems (>4000 m). Alkanes/-enes/-ols contributed between 4% and 30% to the identified SOM composition, with a maximum at mid elevation (2120 m). Fatty acids and fatty acid esters contributed with Two main factors that seem to affect SOM quality and composition at Mt. Kilimanjaro were: 1) the rate and composition of organic matter inputs that in turn are controlled by climatic characteristics and the vegetation type and 2) the decomposition rate and efficiency, mainly controlled by soil pH, temperature and water availability. High forest productivity at mid elevations (2200 m) leads to high amounts of volatile compounds and increases stable SOM pools. The overall carbon accumulation in Andosols of Mt. Kilimanjaro is linked to the percentage of bound lipids (mainly alkanes, alkenes and alcohols), while site specific input patterns (e.g. vegetation or wild fires) are strongly reflected by sterols, lignin derived compounds or polycyclic aromatic compounds in SOM.

Agriculture and the exploitation of natural resources have transformed tropical mountain ecosystems across the world, and the consequences of these transformations for biodiversity and ecosystem functioning are largely unknown1-3. Conclusions that are derived from studies in non-mountainous areas are not suitable for predicting the effects of land-use changes on tropical mountains because the climatic environment rapidly changes with elevation, which may mitigate or amplify the effects of land use4,5. It is of key importance to understand how the interplay of climate and land use constrains biodiversity and ecosystem functions to determine the consequences of global change for mountain ecosystems. Here we show that the interacting effects of climate and land use reshape elevational trends in biodiversity and ecosystem functions on Africa's largest mountain, Mount Kilimanjaro (Tanzania). We find that increasing land-use intensity causes larger losses of plant and animal species richness in the arid lowlands than in humid submontane and montane zones. Increases in land-use intensity are associated with significant changes in the composition of plant, animal and microorganism communities; stronger modifications of plant and animal communities occur in arid and humid ecosystems, respectively. Temperature, precipitation and land use jointly modulate soil properties, nutrient turnover, greenhouse gas emissions, plant biomass and productivity, as well as animal interactions. Our data suggest that the response of ecosystem functions to land-use intensity depends strongly on climate; more-severe changes in ecosystem functioning occur in the arid lowlands and the cold montane zone. Interactions between climate and land use explained-on average-54% of the variation in species richness, species composition and ecosystem functions, whereas only 30% of variation was related to single drivers. Our study reveals that climate can modulate the effects of land use on biodiversity and ecosystem functioning, and points to a lowered resistance of ecosystems in climatically challenging environments to ongoing land-use changes in tropical mountainous regions.

Aims Soil organic carbon (C) efflux is tightly linked to the rhizosphere, where soil microorganisms rapidly decompose organic compounds released from roots. Recently, imaging approaches have greatly improved our understanding of small-scale C-turnover heterogeneity and promoted the term ‘rhizosphere hotspots’ for highly active areas. However, despite often assumed, the effect of these hotspots on total soil C balances is still unknown. We aim to bridge this gap by correlating rhizosphere imaging data to soil respiration on individual plant scale. Methods We grew 17 maize (Zea mays L.) plants in rhizoboxes filled with sandy arable soil. After four weeks, the plants were labelled with 14CO2 and root exudation was visualized and quantified by 14C-imaging one day after labeling. The evolved CO2 was trapped in NaOH and 14CO2 as well as total CO2 was quantified before and after labelling. Enzyme activity (β-glucosidase) was quantified by soil zymography. Results Bulk soil β-glucosidase activitiy negatively correlated to total CO2 efflux, and was the most important predictor (R2 = 0.55). Total and rhizosphere specific 14C-activity were solely correlated to 14CO2 efflux (r = 0.51, r = 0.58). A combination of bulk soil β-glucosidase activity, rhizosphere-14C activity and root biomass, explained about 75% of variance in CO2 efflux. Conclusions This indicates that root exudation and enzyme-activity hotspots are suitable predictors for total soil respiration, particularly when combined with root biomass to account for three-dimensional variation, and that hotspots on the rhizosphere scale are directly linked to larger scale C balances.

Diversity and community composition of soil microorganisms along the elevation climosequences have been widely studied, while the microbial metabolic potential, particularly in regard to carbon (C) cycling, remains unclear. Here, a metagenomic analysis of C related genes along five elevations ranging from 767 to 4190 m at Mount Kilimanjaro was analysed to evaluate the microbial organic C transformation capacities in various ecosystems. The highest gene abundances for decomposition of moderate mineralizable compounds, i.e. carbohydrate esters, chitin and pectin were found at the mid-elevations with hump-shaped pattern, where the genes for decompositions of recalcitrant C (i.e. lignin) and easily mineralizable C (i.e. starch) showed the opposite trend (i.e. U-shaped pattern), due to high soil pH and seasonality in both low and high elevations. Notably, the gene abundances for the decompositions of starch, carbohydrate esters, chitin and lignin had positive relationships with corresponding C compounds, indicating the consistent responses of microbial functional profiles and metabolites to elevation climosequences. Understanding of adaptation of microbial communities, potential function and metabolites to elevation climosequences and their influencing factors provided a new insight for the regulation of terrestrial C storage.

Urban trees as main part of urban green infrastructure provide manifold ecosystem services and contribute to the wellbeing of humans. Unfortunately, urban trees, especially roadside trees, are severely challenged by both, political conflicts of interests in terms of city development and a variety of physically stressors. Contrary to the known benefits of urban green, its proportion in most cities is still decreasing. Furthermore, climate change exacerbates the already challenging preconditions.For northern Germany, climate change is predicted to shift temperature- and precipitation patterns. Simultaneously the frequency of “summer days” and “hot days” are likely to increase, leading to elevated risk of soil drying during the vegetation period.The city of Hamburg is home to almost 220.000 roadside trees. Especially trees planted nowadays are exposed to harsh roadside conditions. In the event of drought, young-trees compared to well-established trees, are not in touch with deep- or distant water reservoirs and the risk of vitality loss or death increases.Our research aims to characterize the soil hydrological conditions in the rooting zone of roadside young-trees during the first years after plantation. Further it aims to identify spatio-temporal dynamics of soil water response during phases of extreme meteorological drought. Our findings are based on a long-term soil water monitoring across the city of Hamburg, which was started in 2016. The monitoring covers 20 trees from 7 species, planted between 2007 and 2019 with large, medium and low soil sealing. Soil water tension and soil temperature were measured hourly with sensors in the root ball, in the tree pit filled with structural soil and the surrounding soil (16 sensors per site).Our data provides a broad characterization of soil water conditions for young-tree sites in urban areas, and show that water supply in years of moderate meteorological drought is not only extremely heterogeneous on large scales, but can also vary greatly on a small scale. The water tension in the root ball, which should provide the highest amount of water per unit, was highly variable and exceeded thresholds even in the first year after plantation and in almost every vegetation period across all sites. In years of high meteorological drought like in 2018, the soil water tensions exceeded the thresholds in almost all compartments, which leads to a risk of vitality losses and mortality.Our data show the need for adaption of general tree site concepts for future plantations. This unique dataset will be further completed with the aim to include future sites and plantation strategies e.g. the underground connection of planting pits, to increase the diversity of site characteristics and to develop reliable modelling and recommendations.

Many experiments have shown that biodiversity enhances ecosystem functioning. However, we have little understanding of how environmental heterogeneity shapes the effect of diversity on ecosystem functioning and to what extent this diversity effect is mediated by variation in species richness or species turnover. This knowledge is crucial to scaling up the results of experiments from local to regional scales. Here we quantify the diversity effect and its components-that is, the contributions of variation in species richness and species turnover-for 22 ecosystem functions of microorganisms, plants and animals across 13 major ecosystem types on Mt Kilimanjaro, Tanzania. Environmental heterogeneity across ecosystem types on average increased the diversity effect from explaining 49% to 72% of the variation in ecosystem functions. In contrast to our expectation, the diversity effect was more strongly mediated by variation in species richness than by species turnover. Our findings reveal that environmental heterogeneity strengthens the relationship between biodiversity and ecosystem functioning and that species richness is a stronger driver of ecosystem functioning than species turnover. Based on a broad range of taxa and ecosystem functions in a non-experimental system, these results are in line with predictions from biodiversity experiments and emphasize that conserving biodiversity is essential for maintaining ecosystem functioning.

International audience; Soil respiration is tightly linked to rhizosphere processes, which are characterized by high spatial variability. We tested whether planar optodes can be applied to capture this spatial variability of CO2 in the rhizosphere. Maize (Zea mays) was grown in rhizoboxes and CO2 concentration around roots was measured at three volumetric soil water contents (VWC) using planar optodes. Gradients of CO2 were clearly visible around root tips but less pronounced around mature root parts probably due to high root respiration and microbial activity around tips. Saturating the soil from 21% VWC increased the measured CO2 concentration from 0.23 to 1.47 μmol CO2 L−1. Statistical comparisons between VWC levels indicated that small changes in soil WC might strongly affect CO2 measurements. However, provided that the soil moisture is kept constant, optode measurements of CO2 can be used in moist soil samples to quantify relative differences in CO2 concentration between treatments or soil regions

Trees as essential components of green urban structures are of crucial importance for the regulation of the urban climate and human wellbeing. Despite this, the currently rising demand for living space and infrastructure causes an increase in the share of sealed and compacted soils. These trends directly affect soil-plant interactions in urban environments. The synergy of the increasing land use pressure and changing climatic conditions worsen the site and growth conditions and thus the vitality for young and mature trees. A possible adaptation strategy is the transformation of plant pits into water reservoirs combining the discharge of excess water with impermeable sole materials and substrates that optimise the water conductivity and storage capacity. The corresponding aim of this study is the quantification of the effects of the water balance dynamic in the rooting zone on the vitality of young trees at highly sealed sites in the city of Hamburg. The two main questions are 1) Do technically modified plant pits reduce summerly drought stress inside the rooting zone and thus improve the root water uptake and tree vitality?, and 2) Does excess water after high rainfall limit the gas exchange and thus the root growth? To answer these questions, we selected two different sites, one residential area and one pedestrian zone, which differ regarding the type of excess water discharge. Overall, two technically modified plant pit variants will be compared with generally constructed variants. Each site will be characterized by soil physical and chemical parameters. Additionally, each plant pit is equipped with TDR- and water tension probes for a continuous monitoring of the soil water balance and O2 as well as CO2 probes for monitoring the gas household. Rhizotrones and dendrometers in combination with Δ13C isotope analysis and stomatal resistance will help to investigate the tree vitality. The data will be used for modelling local water balance dynamics under expected climatic scenarios and for evaluating the different plant pit variants. Development as well as dimensioning recommendations for prospective plant pit constructions, improving the soil-plant interaction, will be derived.

Understanding the influence of habitat properties on the dynamics of terrestrial ecosystems is a fundamental part of ecosystem research under climate change, especially in areas of high environmental heterogeneity, such as floodplains. The present research is part of the collaborative and interdisciplinary MediAN project (Mechanisms of ecosystem services in hardwood floodplain forests: Scientific analysis and optimization of conservation management), where topics regarding carbon storage of soils and tree biomass, as well as tree vitality and the diversity of herbaceous vegetation are investigated. Thus, in this study we aim to understand and characterize the causes and effects of biotic interactions in relation to the edaphic site properties - in particular between the soil water balance and hardwood forests.In this context, we identified the spatiotemporal variability of soil hydro-physical properties in six active and former hardwood floodplain forest sites in the Middle Elbe River, Germany. The study sites represent the floodplain geomorphology, therefore varying in elevation (high and low sites), soil texture and hydrological properties, as well as in forest age. We opened three soil profiles per site and installed soil sensors to monitor the variation of volumetric water content (VWC), and water tension at 10 cm, 30 cm, 60 cm, 100 cm and 160 cm depth. Additionally, we installed one groundwater diver and one meteorological station per site. Undisturbed samples were taken per horizon to define the grain size distribution, bulk density and to evaluate soil moisture retention curves.Preliminary results, from the year 2019 with an unusual dry summer season, show a clear interrelation of soil moisture retention and soil water tension with groundwater level and soil texture. For instance, a predominantly sandy site, located in the high active floodplain, displayed VWC values below 8% throughout the vegetation period and at all depths. Water tension showed higher variation at 10 and 30 cm (2-3.25 pF) compared to deeper soil, which is directly related to precipitation events. These initial observations imply local water stress for the floodplain vegetation; therefore, physiological stress on woody species is expected. In a next step, the dynamics of soil drying will be related to xylem sap flow velocity to estimate effects on the vitality of typical hardwood forest species, such as oaks (Quercus robur L.) and elms (Ulmus laevis) at the representative study sites.

The formation of microrelief forms in peatlands - elevated and dry hummocks, depressed wet hollows and intermediate lawns - is controlled by the interaction of water table, nutrient availability and dominant plant communities. This affects the composition and activity of various functional groups of microorganisms. With depth, the change in peat quality from less to more highly processed organic material additionally regulates microbial activity. We hypothesized that microbial biomass and enzyme activities are driven by aeration and by peat quality and therefore (i) they increase from hollows (water saturated/anaerobic) through lawns (intermediate) to hummocks (aerobic) in the top peat and ii) they decrease with depth due to increasing distance from fresh plant-derived inputs and lower oxygen availability. These hypotheses were tested for enzymes catalysing the decomposition of C-, N-, P- and S-containing organic compounds in peat of the three microform types at three depths (15, 50 and 200 cm). Microbial biomass and peat chemical characteristics were compared with enzyme kinetic parameters, i.e. maximal potential activity (Vmax) and the Michaelis constant (Km). Microbial biomass carbon (MBC) and Vmax of β-glucosidase and N-acetyl glucosaminidase increased by 30–70% from hummocks and lawns to hollows in the top 15 cm, contradicting the hypothesis. Similarly, Km and the catalytic efficiency of enzymes (Ka = Vmax/Km) were best related to MBC distribution and not to the aeration gradient. With depth, Vmax of β-glucosidase, xylosidase and leucine aminopeptidase followed the hypothesized pattern in hollows. In contrast, MBC was 1.3–4 times higher at 50 cm, followed by successively lower contents at 15 and 200 cm in all microforms. The same depth pattern characterized the Vmax distribution of 6 out of 8 enzymes. Phosphatase activity decreased from drier hummock to wetter hollows and the higher activity throughout the peat profile suggested a high microbial demand for P. Enzyme activities and catalytic efficiency in peat were closely linked to the distribution of microbial biomass with depth, which in turn was best explained by P content. From the ecological perspective, these results clearly show that peat decomposition will be accelerated when microbial activity is stimulated e.g. by increased P availability.

We assessed key relationships of soil organic carbon (SOC) stocks to hydromorphic features, soil texture, C/N ratio, pH, and forest age in hardwood floodplain forests. Floodplain ecosystems play a significant role in the global carbon cycle, particularly due to their SOC storage potential. Interactions between heterogeneous micro-relief and anthropogenically affected vegetation and the hydrologic situation of floodplains make predicting SOC stocks difficult without field validation. Especially within hardwood floodplain forests, the controlling factors for SOC storage remain understudied, whereas these floodplain types can be the largest reservoir for carbon and have a substantial SOC sequestration potential. To determine the controlling factors for SOC storage, we selected 38 hardwood floodplain forest and 11 floodplain grasslands of the lower middle Elbe River and categorized them according to vegetation type (grassland, young forest, or old forest) and hydrologic situation (low-lying or high elevated and active or former floodplain). Profile SOC stocks were determined to a depth of 1 m, and topsoil SOC stocks were related to vegetation and soil characteristics, particularly pedological traits (e.g., hydromorphic features). Forest SOC stocks ranged between 99 and 149 t ha−1. SOC stocks decreased with depth throughout all categories and were unaffected by vegetation type within the same hydrologic situation. Vegetation parameters such as age, basal area, or leaf litter carbon stock had no direct effect on SOC stocks. An active connection to the river had the strongest effect on SOC stocks, with former floodplain sites storing 33% less SOC than the active sites. Within the active floodplains, low sites stored 50% more SOC than high sites. This effect was mainly controlled by relief-affected features such as flooding duration and fine texture, which also were the strongest univariate predictors for SOC stocks (R2 = 0.39 and 0.63). A multiple linear regression showed that fine texture, pH, C/N ratio and forest age can be used to explain 86% of variance in SOC stocks. We conclude that proxies for relief and sedimentation (i.e., hydromorphic features, flooding duration, soil texture, C/N ratio, and pH) are the strongest factors controlling SOC stocks in hardwood floodplain forests. Both can be related to allochthonous carbon inputs, increasing SOC stabilization through the accumulation of fine soil particles, and decreasing aerobic carbon mineralization potential through oxygen scarcity, while vegetation plays only a subordinate role.

Savanna ecosystems are increasingly pressured by climate and land-use changes, especially around populous areas such as the Mt. Kilimanjaro region. Savanna vegetation consists of grassland with isolated trees or tree groups and is therefore characterized by high spatial variation and patchiness of canopy cover and aboveground biomass. Both are major regulators for soil ecological properties and soil-atmospheric trace gas exchange (CO2, N2O, CH4), especially in water-limited environments. Our objectives were to determine spatial trends in soil properties and trace gas fluxes during the dry season and to relate above- and belowground processes and attributes. We selected a Savanna plain with vertic soil properties, south east of Mt. Kilimanjaro. Three trees were chosen from each of the two most dominant species: the legume Acacia nilotica and the non-legume Balanites aegyptiaca. For each tree, we selected one transect with nine sampling points, up to a distance of 4 times the crown radius from the stem. At each sampling point, we measured carbon (C) and nitrogen (N) content, δ13C of soil (0–10, 10–30 cm depth) and in plant biomass, soil C and N pools, water content, available nutrients, cation exchange capacity (CEC), temperature, pH, as well as root biomass and greenhouse-gas exchange. Tree species had no effect on soil parameters and gas fluxes under the crown. CEC, C, and N pools decreased up to 50% outside the crown-covered area. Tree leaf litter had a far lower C:N ratio than litter of the C4 grasses. δ13C in soil under the crown shifted about 15% in the direction of tree leaf litter δ13C compared to soil in open area reflecting the tree litter contribution to soil organic matter. The microbial C:N ratio and CO2 efflux were about 30% higher in the open area and strongly dependent on mineral N availability. This indicates N limitation and low microbial C use efficiency in the soil of open grassland areas. We conclude that the spatial structure of aboveground biomass in savanna ecosystems leads to a spatial redistribution of nutrients and thus C mineralization and sequestration. Therefore, the capability of savanna ecosystems to act as C sinks is both directly and indirectly dependent on the abundance of trees, regardless of their N-fixing status.

Tropical alpine ecosystems are identified as the most vulnerable to global environmental change, yet despite their sensitivity they are among the least studied ecosystems in the world. Despite its important role in constraining potential changes to the carbon balance, soil nitrogen (N) turnover and plant availability in high latitude and high altitude ecosystems is still poorly understood. Here we present a first time study on a tropical alpine Helichrysum ecosystem at Mt. Kilimanjaro, Tanzania, which lies at an altitude of 3880 m. Vegetation composition is characterized and major gross N turnover rates are investigated using the 15N pool dilution method for three different vegetation cover types. In addition greenhouse gas exchange (CO2, N2O and CH4) was manually measured using static chambers. Gross N turnover rates and soil CO2 and N2O emissions were generally lower than values reported for temperate ecosystems, but similar to tundra ecosystems. Gross N mineralization, NH4 + immobilization rates, and CO2 emissions were significantly higher on densely vegetated plots than on sparsely vegetated plots. Relative soil N retention was high and increased with vegetation cover, which suggests high competition for available soil N between microbes and plants. Due to high percolation rates, irrigation/rainfall has no impact on N turnover rates and greenhouse gas (GHG) emissions. While soil N2O fluxes were below the detection limit at all plots, soil respiration rates and CH4 uptake rates were higher at the more densely vegetated plots. Only soil respiration rates followed the pronounced diurnal course of air and soil temperature. Overall, our data show a tight N cycle dominated by closely coupled ammonification-NH4 +-immobilization, which is little prone to N losses. Warming could enhance vegetation cover and thus N turnover; however, only narrower C:N ratios due to atmospheric nitrogen deposition may open the N cycle of Helichrysum ecosystems.

Progressive conversion of tropical rainforests to agricultural monocultures in South East Asia increasingly affects landscape types such as riparian areas. The impacts of conversions on soil organic matter (SOM) vary with changing landforms. However, this was often not accounted for in previous studies where SOM in soils in riparian areas was combined with SOM from well-drained adjacent slopes. Because riparian areas have a high carbon (C) storage potential, our objectives were i) to assess their C stocks after conversion to rubber and oil palm plantations in Sumatra (Indonesia) and ii) to compare the impacts of land use conversion on C stocks between riparian and well-drained areas. Average soil C stock losses from the top 30 cm were about 14% and 4% following conversion of riparian forest to rubber and oil palm plantations, respectively, indicating a high resistance of C to mineralization. C losses from well-drained areas were twice as high as from riparian areas after the respective conversion. δ13C values from riparian areas showed clear heterogeneity down soil profiles that was explained i) by alternating oxic and anoxic conditions, leading to reduced SOM and litter decomposition in riparian areas and ii) by mineral sediments and organic matter deposition and accumulation by erosion from adjacent slopes covered by plantations. We conclude that riparian areas are more resilient in terms of soil C storage towards land-use change than well-drained areas because of sediment deposition and reduced oxygen availability. On this basis, we developed a conceptual model of the effects of land-use change and various ecotone characteristics on SOM mineralization in the top- and subsoil of riparian areas.

The mobilization of soil nutrients bound in organic matter is largely mediated by enzymes derived from plants, soil microorganisms and animal residues. Land-use change alters important soil characteristics that may affect the activities of soil enzymes. However, mechanistic understanding of how land use and management practices influence the catalytic properties of enzymes in top- and subsoil are still scarce, especially in African ecosystems. We linked catalytic properties i.e. substrate affinity constant (Km) and maximum reaction rate (Vmax), determined by Michaelis–Menten kinetics, to a set of environmental and microbial variables in the soils of a land-use sequence (6 ecosystems) ranging from natural forests to agricultural fields at Mt. Kilimanjaro. The sensitivity of Km and Vmax of four extracellular hydrolytical enzymes, β-galactosidase, cellobiohydrolase, phosphatase and chitinase to changing environmental conditions were tested by fluorogenic substrates in topsoils and subsoils. The β-galactosidase activity increased with increasing soil depth. Other extracellular enzyme (cellobiohydrolase, phosphatase and chitinase) activities decreased with depth. The affinity of enzymes to substrates was higher in soils of natural compared to agricultural ecosystems: i.e. higher under forests than under cropland. The activity of β-galactosidase, cellobiohydrolase and chitinase enzyme were highest in lower mountain forest and grassland (less disturbed ecosystems). This indicated that changes in land use and management practice not only affects enzyme activity but also controls enzyme kinetics (Km and Ka) thus pointing towards the expression of different enzyme systems. Therefore, we concluded that anthropogenic activities result in alteration of C and nutrient cycling by affecting microbial activities and enzymes catalytic properties.

Agriculture and the exploitation of natural resources have transformed tropical mountain ecosystems across the world, and the consequences of these transformations for biodiversity and ecosystem functioning are largely unknown