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Abstract The incorporation of digital pathology in clinical practice will require the training of pathologists in digital skills. Our study aimed to assess the reliability among pathologists in determining tumor percentage in whole slide images (WSI) of non-small cell lung cancer (NSCLC) using digital image analysis, and study how the results correlate with the molecular findings. Pathologists from nine centers were trained to quantify epithelial tumor cells, tumor-associated stromal cells, and non-neoplastic cells from NSCLC WSI using QuPath. Then, we conducted two consecutive ring trials. In the first trial, analyzing four WSI, reliability between pathologists in the assessment of tumor cell percentage was poor (intraclass correlation coefficient (ICC) 0.09). After performing the first ring trial pathologists received feedback. The second trial, comprising 10 WSI with paired next-generation sequencing results, also showed poor reliability (ICC 0.24). Cases near the recommended 20% visual threshold for molecular techniques exhibited higher values with digital analysis. In the second ring trial reliability slightly improved and human errors were reduced from 5.6% to 1.25%. Most discrepancies arose from subjective tasks, such as the annotation process, suggesting potential improvement with future artificial intelligence solutions.

Con el objetivo del estudio fue determinar la coexistencia y fuentes de alimentación de mosquitos adultos (Diptera: Culicidae) en un centro de salud rural de Piura en Perú, se realizó un estudio descriptivo transversal. Se usaron técnicas entomológicas para capturar e identificar mosquitos, y técnicas de biotecnología molecular para identificar las fuentes de alimentación. Un total de 793 ejemplares de los géneros Culex y Aedes se encontraron coexistiendo, 789 (99,5%) corresponden a Culex quinquefasciatus, 607 (76,9%) fueron machos y 182 (23,1%) hembras. Así mismo, 4 (100%) corresponden a Aedes aegypti hembras. Las fuentes de alimentación de Aedes aegypti fueron Homo sapiens sapiens, y de Culex quinquefasciatus fueron Homo sapiens sapiens y Canis familiaris. Este estudio proporciona evidencia de que los centros de salud rurales estarían actuando como focos de arbovirosis, existiendo el riesgo de que personas que acuden por distintas dolencias, puedan contraer enfermedades transmitidas por C. quinquefasciatus y A. aegypti.

This paper addresses an experience that integrates research and teaching with a gender-based perspective in the qualifying Master of Architecture at the University of Alicante. This experience interweaves heuristic and hermeneutic strategies with the purpose of providing students with methodologies and tools for analysis that encourage a critical approach to the built environment. The study of collective housing complexes created by women architects in Spain is the catalyst for a biographical inquiry and personal encounters with their works and with the residents, in which the interview becomes an instrument to overcome the condition of architecture as an object and explore its epistemic and political fecundity.

Resumen: Antecedentes y objetivo: La enfermedad renal crónica (ERC) se asocia a una elevada morbilidad, carga y consumo de recursos, y constituye un importante desafío para los sistemas sanitarios. El objetivo de este trabajo fue analizar los modelos asistenciales de atención a estos pacientes y consensuar los factores clave que deben implementarse para un modelo óptimo de atención en la ERC, a través de una visión multidisciplinar e integradora. Materiales y métodos: Se conformó un panel multidisciplinar de profesionales con experiencia en el ámbito de la ERC, integrado por un comité asesor (CA) de 15 expertos y un panel adicional de 44 también especialistas. Se identificaron los retos y las áreas de mejora a lo largo de todo el proceso asistencial, a través de la revisión de la evidencia científica y entrevistas individuales al CA. Se consensuaron los factores clave para un modelo óptimo de atención en la ERC que fueron valorados utilizando la metodología de consenso RAND Corporation y Universidad de California en Los Ángeles (RAND/UCLA) (Delphi adaptado), valorando su adecuación y necesidad. Resultados: Se identificaron 38 factores clave para un modelo óptimo de atención al paciente con ERC, estructurados en cuatro retos: 1) desarrollo de modelos de gestión de la ERC y aumento de la visibilidad de la enfermedad, 2) prevención, optimización del cribado, diagnóstico precoz y registro de la ERC en todos los niveles asistenciales, 3) seguimiento integral, multidisciplinar y coordinado, asegurando la optimización terapéutica y la continuidad asistencial, y 4) refuerzo de la formación en ERC a los profesionales sanitarios y pacientes. Un total de 35 factores clave fueron valorados por el panel como adecuados y claramente necesarios, y de estos, 14 fueron considerados altamente prioritarios. Conclusiones: Existe un consenso sobre la necesidad de priorizar la atención de la ERC tanto a nivel institucional como social, avanzando hacia modelos óptimos de atención en ERC basados en la prevención y detección precoz de la enfermedad, así como en el seguimiento integral y coordinado de los pacientes y la formación y concienciación a todos los niveles. Los factores clave identificados constituyen una hoja de ruta implantable en las distintas comunidades autónomas (CCAA) que puede contribuir a una mejora significativa en la atención a estos pacientes. Abstract: Background and Purpose: Chronic kidney disease (CKD) is associated with high morbidity, burden, and resource utilization, and represents a major challenge for healthcare systems. The purpose of this study was to analyse the care patterns for these patients and to reach a consensus on the key factors that should be implemented for an optimal care model in CKD, through a multidisciplinary and integrative vision. Materials and Methods: A multidisciplinary panel of professionals with experience in the field of CKD was formed, composed of an advisory committee of 15 experts and an additional panel of 44 experts. Challenges and areas for improvement across the continuum of care were identified through review of scientific evidence and individual interviews with the advisory committee. Key factors for an optimal model of care in CKD were agreed and assessed using the Rand/UCLA consensus methodology (adapted Delphi), evaluating their appropriateness and necessity. Results: 38 key factors were identified for an optimal CKD patient care model, organised into four challenges: 1) Development of CKD management models and increased visibility of the disease, 2) Prevention, optimisation of screening, early diagnosis and registration of CKD at all levels of care, 3) Comprehensive, multidisciplinary and coordinated monitoring, ensuring therapeutic optimisation and continuity of care, and 4) Reinforcement of CKD training for health care professionals and patients. 35 key factors were assessed by the panel as adequate and clearly necessary, and of these, 14 were considered highly imperative. Conclusions: There is consensus on the need to prioritise CKD care at both institutional and societal levels, moving towards optimal models of CKD care based on prevention and early detection of the disease, as well as comprehensive and coordinated patient monitoring and training and awareness-raising at all levels. The key factors identified constitute a roadmap that can be implemented in the different Autonomous Communities and contribute to a significant improvement in the patient's care.

One of the risks of distraction osteogenesis-based techniques is the development of vascular complications, such as pseudoaneurysms associated with the osteotomies performed or the fixation elements of the external fixator used in the procedure.Pseudoaneurysm are formed when the tunica adventitia of the artery is injured, resulting in a gradual and persistent blood extravasation into the surrounding tissues that is encapsulated and connected to the arterial lumen.This report describes a rare case of a late-presentation pseudoaneurysm in the anterior tibial artery resulting from a tibial lengthening procedure aimed at addressing a leg length discrepancy in a 57-year-old female with severe peripheral neuropathy resulting from long-standing poorly controlled diabetes mellitus. We describe the diagnostic process, the treatment options and confirm how the shape of the bony callus can be a reliable indicator of this pathology, as has already been described in the literature.

Passion fruit (Passiflora edulis) seed extract is a valuable source of antioxidants with anti-aging potential, but extraction efficiency varies according to the method used. This study focused on determining the optimum yield of passion fruit seed extracts by percolation with n-hexane and ethanol, following regulations such as NTE INEN and Codex Alimentarius. Standardized methods were applied to analyze parameters such as moisture (UNE-EN 14774-3, 2010), ash (Sluiter et al.; 2005); free acidity (ac. oleic acidity) / acidity index (NTE INEN 38, 1973), relative density (NTE INEN 35, 1973), loss on heating (NTE INEN 39, 1973), iodine index (NTE INEN 37, 1973), peroxide index (NTE INEN 277,1978), extract yield, fatty acid profile (AOAC 996.06, 2005). Ethanol showed superior performance in most of the parameters, meeting the established standards, except for relative density and heating loss, where n-hexane was more efficient. In conclusion, the use of ethanol is more beneficial for the extraction of passion fruit seed extracts by percolation, based on the yield obtained.

[Objetivo] Verificar el cumplimiento de los niveles de exposición a SO2 y H2S, de los guardaparques del Parque Nacional Volcán Poás, tomando como referencia la norma internacional OSHA. [Metodología] Se estudió la concentración del SO2 desde setiembre 2018, hasta diciembre 2019 y para el H2S de febrero 2019, hasta diciembre 2019, haciendo uso de equipos de detección portátiles MultiRae. Los puntos de muestreo se colocaron en la casa de guardaparques, la caseta de entrada, el centro de visitantes y el mirador. El nivel de riesgo químico se determinó considerando: la salud de los trabajadores, el tiempo de exposición, el equipo de seguridad, las capacitaciones y los valores de TWA y STEL obtenidos. [Resultados] El valor más alto de la concentración de SO2 fue de 16,0 ppm, se registró en el centro de visitantes, y para el de H2S fue de 45 ppm (casa de guardaparques). La concentración promedio de los gases estuvo cercana al límite de detección para ambos gases, sugiriendo que los gases, en general, no se dirigen hacia las zonas de visitación. Ningún puesto de medición superó los 2 ppm para el SO2 en 8 horas (TWA). Sin embargo, en el centro de visitantes y en el mirador para visitantes, se superó los 5 ppm de SO2 en 15 minutos (STEL), con concentraciones de 10,1 ppm y 7,4 ppm, respectivamente. [Conclusiones] Se obtuvieron niveles bajos de riesgo químico, para ambos gases, en los puntos muestreados, a excepción del punto del centro de visitantes, donde el nivel de riesgo fue medio.

Los aldehídos son compuestos presentes en la atmósfera que se forman por la degradación fotoquímica de otros compuestos orgánicos en la tropósfera, estos pueden ser emitidos por fuentes naturales o antropogénicas. [Objetivo] En el presente trabajo se implementó un método analítico para la cuantificación de muestras de aldehídos en matrices como aire y agua de lluvia. [Metodología] El muestreo y el análisis de los aldehídos utilizó la metodología TO-11 de la Agencia de Protección Ambiental de los Estados Unidos (EPA). El muestreo se realizó mediante el uso de cartuchos de adsorción recubiertos con una disolución de 2,4-DNFH, formando una hidrazona con los aldehídos presentes en el agua de lluvia y en el aire. Estos fueron analizados mediante un cromatógrafo de líquido acoplado a un detector UV-Visible (HPLC-UV/Vis). Para la validación de la técnica de análisis y la calidad analítica de los resultados se determinaron: linealidad, sensibilidad, límites de cuantificación, límites de detección, repetibilidad, reproducibilidad y porcentaje de recuperación. En el caso de la repetibilidad, se utilizó la comparación del coeficiente de variación de Horwitz con los porcentajes de desviación estándar relativo (% DER) de las muestras. [Resultados] Los límites de detección obtenidos oscilan entre 0,18 μg/m3 para el acetaldehído y 3,20 μg/m3 en el caso de la acroleína, mientras que los límites de cuantificación están entre 0,62 μg/m3 para el acetaldehído y 4,70 μg/m3 para el heptanal; datos que garantizan la calidad analítica del método. [Conclusiones] En general, el método de análisis para los aldehídos mostró una buena correlación lineal, con valores de R2 igual o mayores a 0,9991 para cada curva de calibración, y valores de porcentajes de desvío relativo menores al 2,25 %, lo cual indica una buena precisión en el análisis.

Background: Ifosfamide-induced Fanconi syndrome is a relatively infrequent complication that generally occurs in young patients with a high cumulative dose of ifosfamide; and is commonly characterized by glycosuria, proteinuria, electrolyte abnormalities, and a normal anion gap metabolic acidosis. Case Presentation: In this study, we present the case of a 16-year-old male patient with of osteosarcoma of the right femur with pulmonary metastasis, who received ifosfamide as part of chemotherapy 1 year and 2 months ago and required hospitalization for cellulitis. During inpatient management, he presented with hypokalemia, hypophosphatemia, polyuria, glycosuria, and proteinuria, by which he was diagnosed with Fanconi syndrome and nephrogenic diabetes insipidus, induced by ifosfamide. Management was focused on the control of the internal environment and use of potassium supplements and potassium-sparing diuretics. Conclusion: Patients receiving ifosfamide should be periodically monitored for kidney function and internal environment to detect any potential complications. It is thus important to carefully observe the cumulative dose of ifosfamide to prevent its associated nephrotoxicity, since its appearance can impoverish the prognosis in patients with neoplasms. Therefore, physicians should always be aware about the possibility of nephrotoxicity development.

Abstract Background Exercise-induced pulmonary haemorrhage (EIPH) in athletic horses is characterized by the presence of blood from the lungs in the tracheobronchial tree after intense exercise. Despite the high prevalence of EIPH in horses, the primary aetiology remains unknown. Variants in the genes encoding CD39 and CD39L1 (ENTPD1 and ENTPD2, respectively) were previously reported as potential genetic causes involved in EIPH pathogenesis. However, the role of these variants in haemostatic functions is unknown. Results To investigate the association between EIPH and missense variants in the ENTPD1 (rs1152296272, rs68621348, and rs68621347) and ENTPD2 genes (rs782872967), 76 Thoroughbred horses diagnosed with EIPH and 56 without clinical signs of EIPH (control group) by trachea-bronchial endoscopy were genotyped. The rs1152296272 and rs68621347 variants were linked, which explained why the same results were found in all horses. Approximately 96% and 95% of the EIPH and control horses, respectively, carried at least one nonreference allele for these variants. In contrast, 100% of the control horses and 96% of the EIPH horses were homozygous for the reference allele for the rs68621348 variant. In the EIPH group, 1.5% of the horses were homozygotes and 24% were heterozygous for the nonreference allele of the rs782872967 variant. In the control group, the nonreference allele of this variant was observed only in heterozygotes (16%). There were no significant differences between groups for any of the variants. Conclusions The variants previously described in the genes encoding the CD39 and CD39L1 enzymes were highly present in the studied population. However, no association was found between the occurrence of EIPH and the presence of these variants in Thoroughbred horses in this study.

Objective: The goal of this systematic review was to identify the mechanisms associated with the enzymatic degradation of collagen and gelatin biomaterials and the possible associated flaws. Methods: Four databases (PubMed, B-On, Cochrane Library, and ResearchGate) were used for the bibliographic search of articles. The research question was formulated using the PCC method, (P): collagen or gelatin sponges, hydrogels, and scaffolds; concept (C): enzymatic degradation of collagen or gelatin sponges, hydrogels, and scaffolds; and context (C): effect of enzymatic action on degradation time of collagen or gelatin sponges, hydrogels, and scaffolds. The search was contextualized according to PRISMA recommendations. The identification and exclusion of evidence followed the PRISMA criteria, with specific inclusion and exclusion factors being stipulated for the selection of articles. The risk of bias assessment was performed using the QUIN Scale. Results: The initial search was composed of 13,830 articles after removing duplicates; 56 articles followed for the full-text reading; 45 were excluded; then, 11 articles were obtained, constituting the results of this systematic review. All studies evaluated the materials using gravimetric analysis, and collagenases were the proteases used for the degradation solution. The materials tested were as follows: human-like collagen (HLC) hydrogel with microbial transglutaminase (MTGase), gelatin sponges subjected to different types of crosslinking, and collagen scaffolds with different types of crosslinking. The period of analysis varied between 0.25 h and 35 days. It was possible to highlight the lack of uniformity in the protocols used, which varied largely, thus influencing the degradation times. The risk of bias was low in nine studies and medium in two studies. Conclusions: This systematic review identified a gap in the literature, highlighting the absence of in vitro studies using human saliva and a collagenase concentration close to the physiological levels to simulate oral dynamics. However, based on existing literature, the mechanisms associated with collagen enzymatic degradation in collagen and gelatin biomaterials were comprehensively understood, answering the first research question postulated. In response to the second research question, the main shortcomings identified in the laboratory evaluation of mechanisms associated with collagen enzymatic degradation in collagen and gelatin biomaterials included the lack of standardization in degradation test protocols; this limited inter-study comparisons, which increased heterogeneity. Additionally, variations in collagenase concentrations and types influenced collagen degradation rates, and inappropriate evaluation intervals hindered the identification of total degradation time.

Abstract Background Male breast cancer (MaBC) has limited data on genomic alterations. We aimed to comprehensively describe and compare MaBC’s genomics with female breast cancer’s (FBC) across subtypes. Methods Using genomic data from Foundation Medicine, we categorized 253 MaBC into estrogen receptor (ER)-positive/human epidermal growth factor receptor 2 (HER2)-negative (n = 210), ER-positive/HER2-positive (n = 22) and triple-negative (n = 20). One ER-negative/HER2-positive case was excluded due to n-of-1. The genomics of the final MaBC cohort (n = 252) were compared to a FBC cohort (n = 2708) stratified by molecular subtype, with adjusted p-values. In the overall MaBC and FBC cohorts, we compared mutational prevalence in cancer susceptibility genes (CSG) (ATM/BRCA1/BRCA2/CHEK2/PALB2). Results Comparing ER-positive/HER2-negative cases, MaBc had increased alterations in GATA3 (26.2% vs. 15.9%, p = 0.005), BRCA2 (13.8% vs. 5.3%, p

Abstract Background While Aboriginal and Torres Strait Islander Australians are less likely to drink any alcohol than other Australians, those who drink are more likely to experience adverse alcohol-related health consequences. In a previous study, providing Aboriginal Community Controlled Health Services (ACCHSs) with training and support increased the odds of clients receiving AUDIT-C alcohol screening. A follow-up study found that these results were maintained for at least two years, but there was large variability in the effectiveness of the intervention between services. In this study, we use services that previously received support as a comparison group to test whether training and support can improve alcohol screening and brief intervention rates among wait-list control ACCHSs. Methods Design: Cluster randomised trial using routinely collected health data. Setting: Australia. Cases: Twenty-two ACCHSs that see at least 1000 clients a year and use Communicare as their practice management software. Intervention and comparator: After initiating support, we compare changes in screening and brief intervention between wait-list control services and services that had previously received support. Measurement: Records of AUDIT-C screening and brief intervention activity in routinely collected data. Results During the reference period we observed 357,257 instances where one of 74,568 clients attended services at least once during a two-monthly data extraction period. Following the start of support, the odds of screening (OR = 0.94 [95% CI 0.67, 1.32], p = 0.74, $$B{F}_{10}$$ $$\approx$$ 0.002) and brief intervention (OR = 1.43 [95% CI 0.69, 2.95], p = 0.34, $$B{F}_{10}$$ $$\approx$$ 0.002) did not improve for the wait-list control group, relative to comparison services. Conclusions We did not replicate the finding that support and training improves AUDIT-C screening rates with wait-list control data. The benefits of support are likely context dependent. Coincidental policy changes may have sensitised services to the effects of support in the earlier phase of the study. Then the COVID-19 pandemic may have made services less open to change in this latest phase. Future efforts could include practice software prompts to alcohol screening and brief intervention, which are less reliant on individual staff time or resources. Trial registration Retrospectively registered on 2018-11-21: ACTRN12618001892202.

Abstract In many parts of the world, domestic and wild animal populations interact at the interface between natural and agricultural ecosystems. Introduced with the first inhabitants arriving from eastern Africa, the bushpig (Potamochoerus larvatus) is the largest living terrestrial mammal in Madagascar. Bushpigs are regularly reported close to human settlements where they damage crops and gardens. As domestic pigs are often raised in free-ranging conditions around the villages, bushpigs and domestic pigs can interact leading to the transmission and circulation of shared swine pathogens that impact both animal and human health. In this study, we characterized the socio-ecological context of bushpig–domestic pig interactions in two different regions of western Madagascar. We conducted participatory mapping sessions and focus group interviews with 65 hunters, 80 pig farmers and 96 crop farmers in 20 fokontany, the smallest administrative unit in Madagascar. After discussing with participants, we gathered information about the spatialization of interactions and their potential geographical drivers. We explored data by performing multiple correspondence analysis and hierarchical clustering on principal components. Based on the reported occurrence or absence of bushpig-domestic pig interactions we were able to classify areas with high or intermediate levels of interactions or no interactions at all. Interactions between the two pig species were reported in only 25% of the fokontany assessed. Even though both suid species were attracted to fruit trees, crops, and water sources, only indirect interactions in those spots were reported. Direct interactions were reported in 10% of cases and referred to interspecific sexual and/or agonistic behavior. The participatory methods used to acquire local knowledge about natural events were confirmed as valuable, low-cost exploratory methods to characterize areas with wild-domestic animal interactions. The results of this study will help plan future studies to characterize the interface between the two species from an ecological or epidemiological perspective using more sensitive and sophisticated ecological approaches.

Abstract Group formation and coordination are fundamental characteristics of living matter, essential for performing tasks and ensuring survival. Interactions between individuals play a key role in group formation, and the impact of resource distributions is a vibrant area of research. As of now, an understanding of how patchy resource distributions determine group dynamics is not yet fully understood. Studying active particles in controlled optical landscapes as energy sources, we demonstrate a non-monotonic dependency of group size on landscape patchiness, with the smallest groups forming when the patches match the active particles’ size. A similar relationship is observed in terms of group stability, evidenced by a reduced rate of individual exchange in patchy environments compared to homogeneous conditions. Reduced group sizes can be beneficial to optimise resources in heterogeneous environments and to control information flow within populations. Our results provide insights into the role of patchy landscapes and uneven energy distributions in active matter and hold implications for refining swarm intelligence algorithms, enhancing crowd management techniques, and tailoring colloidal self-assembly.

Yield data represent a valuable layer for supporting decision-making as they reflect crop management results. Forestry decision-makers often rely on coarse spatial resolution data (e.g., forest inventory plots) despite the availability of modern harvesters that can provide high-resolution forestry yield data. The objectives of this study were to present a method for generating high-resolution Eucalyptus grandis yield data (individual tree-level) and explore their applications, such as correlation analysis with soil attributes to aid nutrient recommendations. Two evaluations were conducted at two sites in Brazil: (a) assessing the positioning accuracy of the global navigation satellite system (GNSS) receiver positioning, and (b) analyzing the yield data and their correlation with the soil attributes. The results indicated that positioning the GNSS receiver at the harvesting head provided higher accuracy than placement at the top of the harvester cabin for individual tree-level data. Reliable yield data were generated despite the GNSS receiver’s increased susceptibility to damage when mounted on a harvest head. The linear correlation analysis between the Eucalyptus grandis yield data and soil attributes showed both negative (Clay, B, S, coarse sand, and potential acidity − H + Al) and positive correlations (K, Mg, pH-SMP, Ca, sum of bases, pH, base saturation, fine sand, total sand, and silt content). This study demonstrates the feasibility of obtaining high-resolution yield data at the individual tree-level and their correlation with soil attributes, providing valuable insights for improving forestry decision-making.

Background: COVID-19-associated pulmonary aspergillosis (CAPA) has emerged as a relatively common complication. Multiple studies described this relationship in critical patients, however its incidence and outcome in other risk groups such as immunosuppressed patients remains unknown. In this sense, we aimed to evaluate the rates and outcomes of CAPA in hematological patients and according to the different hematological malignances, comparing to invasive pulmonary aspergillosis (IPA) in non-COVID-19 ones. Methods: Nationwide, population-based and retrospective observational cohort study including all adult patients with hematological malignancies admitted in Spain since March 1, 2020 to December 31, 2021. The main outcome variable was the diagnosis of IPA during hospitalization in hematological patients with or without COVID-19 at admission. The rate of CAPA compared to IPA in non-COVID-19 patients in each hematological malignancy was also performed, as well as survival curve analysis. Findings: COVID-19 was diagnosed in 3.85 % (4367 out of 113,525) of the hematological adult inpatients. COVID-19 group developed more fungal infections (5.1 % vs. 3 %; p

This note reports the nesting of M. semitorquatus inside a human construction, adding new data on its breeding behavior and diet at the southern limit of its distribution. The study area is located in southern Brazil, about 3 km from Turvo State Park, one of the most important protected areas in southern Brazil. The area is near the Uruguay River, in the municipality of Barra do Guarita, in the northwest region of the state of Rio Grande do Sul. The nest was made inside a human building on an abandoned farm surrounded by a fragmented landscape of forest fragments and cultivated areas. The nest was being used by a female, still incubating the eggs. After the breeding period, and once the adult and young birds left the nest, five pellets and alimentary remains were collected. The alimentary remains found in the nest were composed of bird species, rodents, and a lizard species. Among the birds, we identified three new prey species for the Collared Forest-Falcon: Patagioenas picazuro, Furnarius rufus, and Turdus sp. Therefore, we show that anthropic constructions can be used as alternative nesting sites for this species, which likely foraged in open or agricultural landscapes. These observations demonstrate that the Collared Forest-Falcon has some tolerance to modified environments, an important aspect for its conservation.

Low soil organic matter contents may harm several crops, including okra. However, the incorporation of organic materials has proven effective in increasing the availability of nutrients for plants, among other benefits. This study aimed to evaluate the mineral nutrition and yield of okra after supplying organic material (dry cattle manure) to the soil. Five soil organic matter levels (1.2, 2.0, 2.8, 3.6 and 4.4 %), provided by the addition of cattle manure, were assessed under field conditions in two crop cycles, using randomized blocks, in a 5 × 2 factorial design, with four replications. The leaf contents of N, P, K, Ca, Mg, S, B, Fe, Zn, Cu and Mn and fruit yield were analyzed. The increase in soil organic matter increased the macro and micronutrient contents in the okra leaves; however, excessive levels of soil organic matter reduced the concentrations of N, P, Ca, Mg, S, B, Fe, Mn and Cu, with variations between the crop cycles. Furthermore, the mineral nutrition and plant yield parameters were higher in the second cultivation cycle, when compared to the first one. Fruit yield peaked at 24.9 t ha-1 with 3.8 % of soil organic matter. Therefore, this concentration is recommended to achieve a high okra yield.

Marine mammals are integral to global biodiversity and marine health through their roles in coastal, benthic, and pelagic ecosystems. Marine mammals face escalating threats from climate change, pollution, and human activities, which perturb their oceanic environment. The diverse biology and extreme adaptations evolved by marine mammals make them important study subjects for understanding anthropogenic pressures on marine ecosystems. However, ethical and logistical constraints restrict the tractability of experimental research with live marine mammals. Additionally, studies on the effects of changing ocean environments are further complicated by intricate gene-environment interactions across populations and species. These obstacles can be overcome with a comprehensive strategy that involves a systems-level approach integrating genotype to phenotype using rigorously defined experimental conditions in vitro and ex vivo. A thorough analysis of the interactions between the genetics of marine mammals and their exposure to anthropogenic pressures will enable robust predictions about how global environmental changes will affect their health and populations. In this perspective, we discuss four challenges of implementing such non-invasive approaches across scientific fields and international borders: 1) practical and ethical limitations of in vivo experimentation with marine mammals, 2) accessibility to relevant tissue samples and cell cultures; 3) open access to harmonized methods and datasets and 4) ethical and equitable research practices. Successful implementation of the proposed approach has the potential impact to inspire new solutions and strategies for marine conservation.

Urban areas are experiencing rapid growth, accompanied by significant disorder in Lima Metropolitan area and many other cities in South America. Due to decades of uncontrolled construction practices, it is imperative to identify and better understand the types of informalities prevalent in these recent urban areas. Addressing this lack of information is crucial for implementing appropriate countermeasures and developing new policies that benefit the communities residing in such areas. It is worth noting that understanding disaster risk aligns with the first priority of the Sendai Framework for Disaster Risk Reduction. In this study, we propose the use of radar satellite imagery recorded by the Sentinel-1 constellation since 2017 to identify clusters of urban growth in Lima Metropolitan area. Then, the informal urban clusters can be depicted by visual inspection of the last recorded high-resolution optical image. With good spatial and temporal resolution, we identified 25 clusters informal areas. Among our findings, we observed that several of these clusters are situated in landfills comprised of construction and other waste, increasing their vulnerability to debris flow, landslides, and earthquakes. Additionally, we noted that some new urban areas mainly consist of temporarily empty houses, highlighting the feasibility of implementing countermeasures, such as relocations, in the early stages of informal occupation. These results underscore the significant contribution of satellite radar imagery in identifying recent informal urban areas.

While pesticide use is subject to strict regulatory oversight worldwide, it remains a main concern for environmental protection, including biodiversity conservation. This is partly due to the current regulatory approach that relies on separate assessments for each single pesticide, crop use, and non-target organism group at local scales. Such assessments tend to overlook the combined effects of overall pesticide usage at larger spatial scales. Integrative landscape-based approaches are emerging, enabling the consideration of agricultural management, the environmental characteristics, and the combined effects of pesticides applied in a same or in different crops within an area. These developments offer the opportunity to deliver informative risk predictions relevant for different decision contexts including their connection to larger spatial scales and to combine environmental risks of pesticides, with those from other environmental stressors. We discuss the needs, challenges, opportunities and available tools for implementing landscape-based approaches for prospective and retrospective pesticide Environmental Risk Assessments (ERA). A set of “building blocks” that emerged from the discussions have been integrated into a conceptual framework. The framework includes elements to facilitate its implementation, in particular: flexibility to address the needs of relevant users and stakeholders; means to address the inherent complexity of environmental systems; connections to make use of and integrate data derived from monitoring programs; and options for validation and approaches to facilitate future use in a regulatory context. The conceptual model can be applied to existing ERA methodologies, facilitating its comparability, and highlighting interoperability drivers at landscape level. The benefits of landscape-based pesticide ERA extend beyond regulation. Linking and validating risk predictions with relevant environmental impacts under a solid science-based approach will support the setting of protection goals and the formulation of sustainable agricultural strategies. Moreover, landscape ERA offers a communication tool on realistic pesticide impacts in a multistressors environment for stakeholders and citizens.

La dinámica digital se ha caracterizado por el acoso, amenazas, insultos, vulneración de datos privados son prácticas de todos los días en el mundo entero, principalmente de hombres contra las mujeres. Dicho fenómeno está siendo estudiado desde diferentes focos y no debe ser normalizada ni minimizada en la carrera hacia la transformación digital del mundo físico. Solo en Costa Rica, en el último año, se registraron 1.405.668 conversaciones con odio y lenguaje discriminatorio, lo que significa un incremento del 255 % en relación con 2021. La presente investigación analiza el 100 % de la escucha digital política de Costa Rica durante un año, es decir, un volumen de 495.257 menciones públicas sin spam de troles y bots, mediante técnicas que combinan tecnología e ingenio humano. Las mujeres políticas y lideresas costarricenses que intervienen en el debate de ideas son quienes más violencia padecen por diferentes causales que se examinan, con base en decisiones y retóricas coyunturales de la política nacional, al tiempo que la violencia digital permanece como práctica estructural que se intensifica cada día con consecuencias reales y totalizantes de nuevo en el mundo material. Se reflexiona acá sobre dicha realidad y se aportan seis tácticas para cimentar una contranarrativa al odio cultural offline que se ha imbricado en lo digital.

The crucial role of soil in global food production and its multifaceted contributions to ecosystem services underscore the need for a comprehensive evaluation framework. This study presents a novel approach to soil evaluation by integrating the assessment of soil functions, services, and threats into a unified metric which quantifies the capital dimension which is termed ''soil management capital''. To achieve this, we focused on three key roles: a threat, evaluated by the pH regulation costs, factoring in different buffering capacities and liming/sulphuring treatments to attain an optimal reference pH, a function, representing soil's role as a reservoir and regulator of nutrients; and a service, considering available water holding capacity (AWC) for water security. Integrating these activities culminates in a soil capital metric, providing a comprehensive understanding of the soil's economic value in US$/ha units on a pixel-by-pixel basis across Australia. To achieve this, we analysed spatial information from the Soil and Landscape Grid of Australia (SLGA) with Google Earth Engine (GEE). Our study generated detailed maps illustrating pH regulation costs, OC, nutrients (N, P, K), and AWC across Australia. The integration of these roles revealed regions of large soil capital in the Australian eastern coast and Tasmania.

Diabetes mellitus and its complications are a known public health problem nowadays. Diabetic nephropathy is one of the main complications and the result of multiple mechanisms, including: activation of the renin-angiotensin-aldosterone system, formation of advanced glycation end products and chronic inflammation that led to glomerular and tubulo-interstitial damage producing mesangial expansion and glomerulosclerosis, which finally results in chronic kidney disease. Early detection of diabetic nephropathyis essential for adequate intervention to stop, or at least sloww down its progression. Multiple markers have been described, not only the classic ones such as serum creatinine, urea, and albuminuria, but at this point also novel biomarkers such as neutrophil gelatinase-associated lipocalin, tumor necrosis factor 1 receptor and monocyte chemoattractant protein-1, among others. The aim of this article was to provide an update review of the role of biomarkers in the diagnosis of diabetic nephropathy.

Abstract Eutrophication is a main threat to continental aquatic ecosystems. Prevention and amelioration actions have been taken under the assumption of a stable climate, which needs reconsideration. Here, we show that reduced precipitation can bring a lake ecosystem to a more productive regime even with a decline in nutrient external load. By analyzing time series of several decades in the largest lake of the Iberian Peninsula, we found autocorrelated changes in the variance of state variables (i.e., chlorophyll and oxygen) indicative of a transient situation towards a new ecosystem regime. Indeed, exceptional planktonic diatom blooms have occurred during the last few years, and the sediment record shows a shift in phytoplankton composition and an increase in nutrient retention. Reduced precipitation almost doubled the water residence time in the lake, enhancing the relevance of internal processes. This study demonstrates that ecological quality targets for aquatic ecosystems must be tailored to the changing climatic conditions for appropriate stewardship.

Abstract Background In yeasts belonging to the subphylum Saccharomycotina, genes encoding components of the main metabolic pathways, like alcoholic fermentation, are usually conserved. However, in fructophilic species belonging to the floral Wickerhamiella and Starmerella genera (W/S clade), alcoholic fermentation was uniquely shaped by events of gene loss and horizontal gene transfer (HGT). Results Because HGT and gene losses were first identified when only eight W/S-clade genomes were available, we collected publicly available genome data and sequenced the genomes of 36 additional species. A total of 63 genomes, representing most of the species described in the clade, were included in the analyses. Firstly, we inferred the phylogenomic tree of the clade and inspected the genomes for the presence of HGT-derived genes involved in fructophily and alcoholic fermentation. We predicted nine independent HGT events and several instances of secondary loss pertaining to both pathways. To investigate the possible links between gene loss and acquisition events and evolution of sugar metabolism, we conducted phenotypic characterization of 42 W/S-clade species including estimates of sugar consumption rates and fermentation byproduct formation. In some instances, the reconciliation of genotypes and phenotypes yielded unexpected results, such as the discovery of fructophily in the absence of the cornerstone gene (FFZ1) and robust alcoholic fermentation in the absence of the respective canonical pathway. Conclusions These observations suggest that reinstatement of alcoholic fermentation in the W/S clade triggered a surge of innovation that goes beyond the utilization of xenologous enzymes, with fructose metabolism playing a key role.

Bioluminescence is the ability of organisms to emit their own light which can be self-produced in light organs by photogenic cells (photocytes) or symbiotically by bearing cultures of luminous bacteria. Luminous animals are mainly comprised in the ocean, nocturnal coastal shallow-water species or, as the majority of species, inhabiting deep-sea where dim light conditions are optimal for communication by luminous signals. Due to difficult access to species, fisheries of bioluminescent fishes worldwide are rare, mostly for traditional cuisine and fishing bait. Fishes are the only bioluminescent vertebrates and thus their light organs present an astonishing level of complexity for light modulation to adequate light signals for specific ecological roles. Fishes control the output of light intensity using different mechanisms which can be categorized as being under control of hormonal or neuronal pathways. Usually, neural control allows fast and direct response to the photocytes while endocrinal systems allow to control the light produced by bacteria, via manipulation of individual melanophores. In some species, mechanical movement of melanophore-filled tissues by muscular action is under neuronal or hormonal control. Sexual dimorphism of light organs, driven by hormonal regulation during ontogeny, takes a crucial role in communication and reproductive success of these species.

Over the years, agricultural management practices are being improved as they integrate Information and Communication Technologies (ICT) and Precision Agriculture tools. Regarding sugarcane crop production, this integration aims to reduce production cost, enhance input applications, and allow communication among different hardware and datasets, improving system sustainability. Sugarcane mechanization has some particularities that mandate the development of custom solutions based on digital tools, which are being applied globally in different crops. Digital mechanization can be conceived as the application of digital tools on mechanical operation. This review paper addresses different digital solutions that have contributed towards the mechanization of sugarcane crop production. The process of digitalization and transformation in agriculture and its related operations to sugarcane are presented, highlighting important ICT applications such as real-time mechanical operations monitoring and integration among operations, demonstrating their contributions and limitations regarding management efficiency. In addition, this article presents the major challenges to overcome and possible guidance on research to address these issues, i.e., poor communication technologies available, need for more focus on field and crop data, and lack of data interoperability among mechanized systems.

Abstract Background Elephant seals exhibit extreme hypoxemic tolerance derived from repetitive hypoxia/reoxygenation episodes they experience during diving bouts. Real-time assessment of the molecular changes underlying protection against hypoxic injury in seals remains restricted by their at-sea inaccessibility. Hence, we developed a proliferative arterial endothelial cell culture model from elephant seals and used RNA-seq, functional assays, and confocal microscopy to assess the molecular response to prolonged hypoxia. Results Seal and human endothelial cells exposed to 1% O2 for up to 6 h respond differently to acute and prolonged hypoxia. Seal cells decouple stabilization of the hypoxia-sensitive transcriptional regulator HIF-1α from angiogenic signaling. Rapid upregulation of genes involved in glutathione (GSH) metabolism supports the maintenance of GSH pools, and intracellular succinate increases in seal but not human cells. High maximal and spare respiratory capacity in seal cells after hypoxia exposure occurs in concert with increasing mitochondrial branch length and independent from major changes in extracellular acidification rate, suggesting that seal cells recover oxidative metabolism without significant glycolytic dependency after hypoxia exposure. Conclusions We found that the glutathione antioxidant system is upregulated in seal endothelial cells during hypoxia, while this system remains static in comparable human cells. Furthermore, we found that in contrast to human cells, hypoxia exposure rapidly activates HIF-1 in seal cells, but this response is decoupled from the canonical angiogenesis pathway. These results highlight the unique mechanisms that confer extraordinary tolerance to limited oxygen availability in a champion diving mammal.

Objetivo: La acción de tutela es un instrumento creado con la Constitución de 1991 y constituye un indicador de fallo en el sistema de salud. En 2015 se promulgó la Ley Estatutaria, con la cual se regula el derecho a la salud. Este trabajo analiza los efectos de dicha ley sobre el comportamiento de las tutelas en salud entre 2008 y 2019. Metodología:Se realizó un estudio cuantitativo, con un diseño cuasiexperimental de series de tiempo interrumpidas de las acciones de tutelas en Colombia, a partir de datos provistos por la Defensoría del Pueblo. Resultados: Entre 2008 y 2019, se instauraron 1 719 584 tutelas en salud; estas incrementaron en el 41 % después de la sanción de la Ley. El número de tutelas mensuales pasó de 8714 antes de la Ley, a 12 295 después de su sanción; la diferencia fue de 3581,37 95% (IC 5938,12; 1224,62), con una pendiente positiva de 96,13 cambios que son estadísticamente significativos. Conclusiones: Las medidas adoptadas a partir de la Ley Estatutaria han sido insuficientes para corregir la tendencia creciente de las tutelas de salud en Colombia; contrario a lo esperado, estas han aumentado desde su promulgación. A pesar del cambio en los valores e ideas impulsados por el poder judicial, esta ley no logró una transformación del arreglo institucional, lo que se manifiesta en la persistencia de la tutela como mecanismo para lograr el acceso y goce efectivo a la atención sanitaria. Este análisis aporta un conjunto de elementos —una combinación de aspectos estructurales, funcionales y de la cultura producida— que podrían explicar dicho comportamiento.

Abstract Increased recruitment of transitional and non-classical monocytes in the lung during SARS-CoV-2 infection is associated with COVID-19 severity. However, whether specific innate sensors mediate the activation or differentiation of monocytes in response to different SARS-CoV-2 proteins remain poorly characterized. Here, we show that SARS-CoV-2 Spike 1 but not nucleoprotein induce differentiation of monocytes into transitional or non-classical subsets from both peripheral blood and COVID-19 bronchoalveolar lavage samples in a NFκB-dependent manner, but this process does not require inflammasome activation. However, NLRP3 and NLRC4 differentially regulated CD86 expression in monocytes in response to Spike 1 and Nucleoprotein, respectively. Moreover, monocytes exposed to Spike 1 induce significantly higher proportions of Th1 and Th17 CD4 + T cells. In contrast, monocytes exposed to Nucleoprotein reduce the degranulation of CD8 + T cells from severe COVID-19 patients. Our study provides insights in the differential impact of innate sensors in regulating monocytes in response to different SARS-CoV-2 proteins, which might be useful to better understand COVID-19 immunopathology and identify therapeutic targets.

Adolescent pregnancy poses a challenge due to clinical and social risks, increasing the likelihood of low adherence to prenatal care and violence. The COVID-19 pandemic significantly impacted obstetric care in Brazil, possibly increasing maternal death and caesarean section rates (CSR) in Brazilian adolescent pregnancies. We performed a cross-sectional study analysing births and maternal deaths in Brazil in 2019 (pre-pandemic) and 2021 (pandemic). Data were obtained from the Brazilian Panels of Monitoring Live Births and Maternal Mortality. Data on number of births, maternal mortality rate (MMR), and CSR were compared in both periods. There was a significant reduction in adolescent births during the pandemic (13.62% in 2021 vs. 14.72% in 2019). The MMR increased from 46.75 to 62.79 per 100,000 live births, due to respiratory infections (23.98% to 43.67%). The CSR increased from 38.39% to 40.25%. The results suggest an increase in CSR and MMR among Brazilian adolescents during COVID-19 pandemic.

Single-cell RNA sequencing (scRNA-seq) technology has emerged as a powerful tool for dissecting cellular heterogeneity and understanding the intricate biology of diseases, including cancer. Endometrial cancer (EC) stands out as the most prevalent gynecological malignancy in Europe and the second most diagnosed worldwide, yet its cellular complexity remains poorly understood. In this review, we explore the contributions of scRNA-seq studies to shed light on the tumor cells and cellular landscape of EC. We discuss the diverse tumoral and microenvironmental populations identified through scRNA-seq, highlighting the implications for understanding disease progression. Furthermore, we address potential limitations inherent in scRNA-seq studies, such as technical biases and sample size constraints, emphasizing the need for larger-scale research encompassing a broader spectrum of EC histological subtypes. Notably, a significant proportion of scRNA-seq analyses have focused on primary endometrioid carcinoma tumors, underscoring the need to incorporate additional histological and aggressive types to comprehensively capture the heterogeneity of EC. By critically evaluating the current state of scRNA-seq research in EC, this review underscores the importance of advancing towards more comprehensive studies to accelerate our understanding of this complex disease.

En este artículo se trabaja filosóficamente el autorretrato –selfi– producido por los smartphones y su publicación en las redes sociales. Para esto se reflexiona sobre cuestiones de carácter técnico a la luz de lo propuesto por autores como Vilém Flusser, Gilbert Simondon y Gerard Wajcman. Luego, siguiendo la perspectiva psicoanalítica de Jacques Lacan, se aborda la necesidad de la imagen en un sentido estructural del individuo, asunto proyectado hacia la proliferación del autorretrato en Internet. Finalmente, a partir del pensamiento filosófico de Patricio Marchant, se analiza la compulsión fotográfica contemporánea por medio de conceptos tales como donación y envío.

This paper proposes a set of digitalisation indicators focused on measuring the different digital capabilities and infrastructure of electricity distribution systems, as opposed to previous indicators which have mainly focused on performance and quality of service aspects.The indicators are classified according to the pillars of digitalisation: sensor and actuator, connectivity, data processing, and digital culture. They are use-case-agnostic and do not require a huge amount of information. In addition to this, three possible new applications of these indicators for distribution system operators and regulatory authorities are identified and discussed.The extensive use of these indicators in Europe could allow the development of fruitful collaborations between distribution system operators, allow the identification of cause–effect relations between grid performance and digital infrastructure, and improve the replicability of innovative smart grid solutions. However, this will only be possible if regulators promote the adoption of the proposed indicators and the dissemination of their results.

Strawberries in California are grown in specific coastal areas where land is scarce and climate change threatens future production. Strawberry growers are under pressure to adopt sustainable production strategies such as crop rotation, but this practice requires more land than back-to-back planting. The objectives of this research were to quantify the rate of crop rotation across the three main strawberry producing regions in California (Ventura, Santa Maria, and Monterey Bay), and evaluate geographic and edaphic influences on crop rotation. All strawberry fields in the main strawberry producing regions of California were identified by satellite imagery and manual inspection for the years 2017 through 2022. ArcGIS Pro was used to outline each strawberry field and compare among years to determine the period between successive strawberry plantings. Edaphic characteristics and shapefiles for surrounding fields were retrieved from public datasets. The three regions significantly differed in their rates of crop rotation. On average, 95, 52, and 25% of strawberry hectares were rotated each year in the Monterey Bay, Santa Maria, and Ventura regions, respectively. Shorter rotation lengths were associated with reduced distance from the ocean and soil with a higher percentage of sand. Based on 2 years of disease surveys, fields infested with Macrophomina phaseolina tended to be further inland than fields infested with Fusarium oxysporum f. sp. fragariae in the Monterey Bay region. This study determined that distance from the ocean and soil texture are associated with crop rotation lengths in California strawberry production. Enough land may be available in the Santa Maria and Monterey Bay regions for growers to lengthen crop rotations, but water quality, social networks, and financial considerations that were outside the scope of this study are likely to limit the ability for strawberry growers to maximize the duration of crop rotations.

Rhipicephalus microplus, es la especie parasitaria que más daño genera a la ganadería mexicana y global debido a las pérdidas directas e indirectas, como lo es el incremento de la multirresistencia y la resistencia cruzada. En la actualidad, son pocos los estudios que se tienen sobre la resistencia a lactonas macrocíclicas en México, siendo su mayoría en el sur. El presente estudio tuvo como objetivo evaluar el estado de la resistencia a ivermectina en R. microplus, en el noreste de México y factores de riesgo asociados a ésta. Se colectaron 20 poblaciones de Rhipicephalus microplus, en los estados de Veracruz, Nuevo León, Tamaulipas y San Luis Potosí, y se analizaron con la prueba de inmersión de larvas. Los datos de mortalidad se sometieron a un análisis Probit, estimándose las concentraciones letales (CL) del 50% y 99% y sus respectivos intervalos de confianza del 95% (IC95%) y para la determinación de los posibles factores de riesgo se realizó un análisis multivariado y tablas de contingencia de 2 x 2 para las variables de exposición, con un intervalo de confianza del 95%, y un modelo de regresión logística binomial para aquellas variables con una P≤0.05. El 80 % de las poblaciones analizadas presentaron resistencia con rangos de RR50= 2.07-11.14 y RR99= 3.03-47.93 (P≤0.05) y mediante la regresión logística binomial, se pudo observar que la variable frecuencia de tratamientos obtuvo una P≤0.0134, resultado que mostró ser significativo.

Osteoarthritis (OA) is characterized by articular cartilage degeneration, synovial inflammation, and subchondral bone thickening, affecting the synovial joint as an organ and leading to pain and disability. Subcutaneous stromal vascular fraction is safe and relieves pain, improves function, and repairs cartilage defects in patients with knee OA. Our goal is to describe step-by-step the arthroscopic treatment of mild to moderate knee OA with photo-stimulated stromal vascular fraction harvested from the thigh using a selective tissue engineering photo-stimulation (“One S.T.E.P.”) technique.

Worldwide, approximately one fifth of all cases of diarrhea are associated with norovirus, mainly in children, with a defined seasonality in temperate climates, but seasonal dynamics are less known in tropical climates. The objective was to investigate the impact of external clinical, epidemiological, and climatic factors on norovirus detection rates in samples from children under 5 years of age from Roraima, the Amazon region of Brazil. A total of 941 samples were included. According to climatic factors, we observed correlations between external climatic factors and weekly positivity rates, where temperature (P = 0.002), relative humidity (P = 0.0005), absolute humidity (P

O objetivo do estudo é o de compreender o papel das mesoinstituições e meso-organizações, em complementaridade com a ECM, nas possibilidades de implementação do Selo Arte no Paraná, visando a impulsionar a comercialização de produtos artesanais da agroindústria familiar, da região da AMUSEP. As proposições teóricas da tese visaram a complementariedade entre análise mesoinstitucional e as ferramentas teóricas da Economia de Custos de Mensuração (ECM), com o objetivo de melhor compreender a política pública do Selo ARTE e suas possibilidades de implementação. A proposta metodológica foi de utilizar a análise de conteúdo temática, utilizando-se de categorias mais abrangentes, a fim de preenchê-las a partir da coleta de dados e, com isso, através de uma estratégia de “análise de conteúdo temática direcionada”, onde houve um direcionamento dos assuntos abordados na entrevista a partir do que mais se destacou na conceitualização de cada tema. Para coleta de dados, neste trabalho elaborou-se inicialmente uma coleta de dados secundários e, posteriormente, utilizou-se de dados primários. Como resultado da pesquisa foi possível compreender, através dos dados, quais as mesoinstituições e meso-organizações que envolvem o Selo Arte, e como a ECM atua em complementaridade das funções mesoinstitucionais, a ausência da função de tradução no caso estudado e sua importância para compreender os conflitos e as ineficiências identificadas nas possibilidades de aplicação desta política no Paraná e na região da AMUSEP. A conclusão do estudo é a de que é possível estudar mesoinstituições e ECM em complementaridade, e de que a função mesoinstitucional de tradução quando ausente é capaz de causar conflito entre meso-organizações e ineficiência na implementação de políticas públicas. Abstract: The objective of the study is to understand the role of meso-institutions and meso-organizations, in complementarity with the ECM, in the possibilities of implementing the ARTE Label in the AMUSEP region. The theoretical propositions aimed at the complementarity between meso- institutional analysis and the theoretical tools of the Measurement Costs Economics (MCE). The methodological proposal was to use thematic content analysis. For data collection, in this work, a collection of secondary data was initially elaborated and, later, primary data was used. As a result of the research, it was possible to understand, through the data, which are the meso-institutions and meso- organizations that involve ARTE Label how the MCE acts in complementarity of the meso-institutional functions, the absence of the translation function in the case studied and its importance to understand the conflicts and inefficiencies identified in the possibilities of application of this policy in Paraná and in the AMUSEP region.

Introducción: Comprender el impacto de las carreteras en la fauna es crucial para implementar medidas de protección; Costa Rica es uno de los países tropicales más estudiados en este aspecto, pero algunas áreas siguen siendo poco conocidas. Objetivo: Evaluar la relación entre la frecuencia de atropellos de fauna, el trazado de la carretera y la distancia a la vegetación. Métodos: Trabajamos durante 15km del 8 al 10 de abril de 2016 en la Carretera Nacional Pacífica Fernández, Puntarenas, Costa Rica. Contamos todos los atropellos visibles. Resultados: Registramos 56 individuos atropellados, principalmente sapos (Rhinella horribilis) e iguánidos. La vegetación cercana aumentó el número de víctimas, mientras que no hubo diferencias entre los tramos rectos y curvos de la carretera. Conclusiones: Recomendamos la implementación de barreras, reductores de velocidad y pasos de fauna adecuados, especialmente en áreas con vegetación densa cerca de la carretera.

Nowadays there is an urgent need for mitigating CO2 emissions through clean energy and the development of new carbon capture and utilization (CCU) technologies. Among others, the use of bifunctional ionic liquids (ILs) addressed simultaneously CO2 capture and conversion steps, having applied successfully to the propylene carbonate production case. In this work, a systematic evaluation of all representative cyclic carbonate literature was made, covering ethylene, propylene, butylene, hexylene, cyclohexene, and styrene cyclic carbonates, in order to guide the product role within the integrated CCU (ICCU) concept. The multiscale strategy combining molecular simulation (DFT -Density Functional Theory-, COSMO -COnductor-like Screening MOdel-), process simulation (COSMO/Aspen methodology), and life cycle assessment (LCA) was used to set up, simulate and evaluate the processes. ICCU configuration is the best approach when compared with sequential configuration for energy consumption analysis (reduction of 28, 28, 22, 11 and 6 %, respectively, for ethylene, propylene, butylene, hexylene, and cyclohexene cases) and CO2 emissions associated (reduction of 38, 40, 31 and 14 %, respectively, for ethylene, propylene, butylene, and hexylene cases). The main variable of the results is the boiling point of the cyclic carbonate since heavy products impose technical limitations and even discard ICCU alternative. The ICCU concept works since all cyclic carbonates’ reaction enthalpies are higher than that of the IL-CO2 one, which reduces heating requirements. Finally, energy demand can be slightly further reduced, partially recycling the cyclic carbonate to the capture unit.

The monophyly of Theraphosinae is supported by both morphological and molecular phylogenies. However, intergeneric relationships often show polytomies and branches with low support. A previous phylogenetic study proposed an intergeneric relationship for the subfamily based on molecular data and divided it into three tribes: Grammostolini, Hapalopini and Theraphosini. However, not all genera of Theraphosinae were sampled, and some relationships were inferred based on morphological similarities. Regarding the Hapalopini from Brazil, the relationships of some genera are still uncertain, such as Kochiana, Catanduba, and Munduruku. In this paper, we describe four new species of Hapalopini from Brazil: Cyriocosmus paresi sp. nov., Hapalopus akroa sp. nov., H. guidonae sp. nov., and K. fukushimae sp. nov. In addition, we propose an updated diagnosis for these three genera and for the species K. brunnipes and M. bicoloratum.