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4 results on '"Jose-Carlos Pastor Jimeno"'

1. Evaluation of the neuroprotective efficacy of the gramine derivative ITH12657 against NMDA-induced excitotoxicity in the rat retina

Author

Johnny Di Pierdomenico, Alejandro Gallego-Ortega, María Norte-Muñoz, Beatriz Vidal-Villegas, Isaac Bravo, María Boluda-Ruiz, Jose Manuel Bernal-Garro, Iván Fernandez-Bueno, Jose Carlos Pastor-Jimeno, María Paz Villegas-Pérez, Marcelino Avilés-Trigueros, Cristobal de los Ríos, and Manuel Vidal-Sanz

Subjects
NMDA excitotoxicity, glaucoma, retina ganglion cell, neuroprotection, Brn3a + RGCs, SD-OCT, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571, Human anatomy, QM1-695
Abstract

PurposeThe aim of this study was to investigate, the neuroprotective effects of a new Gramine derivative named: ITH12657, in a model of retinal excitotoxicity induced by intravitreal injection of NMDA.MethodsAdult Sprague Dawley rats received an intravitreal injection of 100 mM NMDA in their left eye and were treated daily with subcutaneous injections of ITH12657 or vehicle. The best dose–response, therapeutic window study, and optimal treatment duration of ITH12657 were studied. Based on the best survival of Brn3a + RGCs obtained from the above-mentioned studies, the protective effects of ITH12657 were studied in vivo (retinal thickness and full-field Electroretinography), and ex vivo by quantifying the surviving population of Brn3a + RGCs, αRGCs and their subtypes α-ONsRGCs, α-ONtRGCs, and α-OFFRGCs.ResultsAdministration of 10 mg/kg ITH12657, starting 12 h before NMDA injection and dispensed for 3 days, resulted in the best significant protection of Brn3a + RGCs against NMDA-induced excitotoxicity. In vivo, ITH12657-treated rats showed significant preservation of retinal thickness and functional protection against NMDA-induced retinal excitotoxicity. Ex vivo results showed that ITH12657 afforded a significant protection against NMDA-induced excitotoxicity for the populations of Brn3a + RGC, αRGC, and αONs-RGC, but not for the population of αOFF-RGC, while the population of α-ONtRGC was fully resistant to NMDA-induced excitotoxicity.ConclusionSubcutaneous administration of ITH12657 at 10 mg/kg, initiated 12 h before NMDA-induced retinal injury and continued for 3 days, resulted in the best protection of Brn3a + RGCs, αRGC, and αONs-RGC against excitotoxicity-induced RGC death. The population of αOFF-RGCs was extremely sensitive while α-ONtRGCs were fully resistant to NMDA-induced excitotoxicity.

Published
2024
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2. Analysis of Lymphotoxin Alpha Expression in Human Retina and Generation of Expression Vectors to Functional Characterization of Polymorphisms in the Tumor Necrosis Factor Locus

Author

Rogelio González-Sarmiento, Salvador Pastor-Idoate, Irene Rodriguez-Hernandez, Ricardo Usategui-Martín, Eva M Sobas, and Jose Carlos Pastor-Jimeno

Subjects
Genetics, Lymphotoxin alpha, Site-directed mutagenesis, Expression vector, Tumor necrosis factor, Locus (genetics), Transfection, Functional characterization, Biology, Retina, Regulatory sequence, LTA, Tumor necrosis factor alpha, Allele, Expression vectors and pCEFL-Flag, Polymorphisms, Gene
Abstract

With the evolution of new genomic sequencing technologies an important amount of genomic data has been provided. As a consequence of this, many gene polymorphisms have been shown to be significantly associated with different disorders. Many strategies have been implemented to reveal the role of having more than one allele at a specific locus and their involvement in the illnesses. Site-directed mutagenesis is one of the most common strategies to understand the regulatory regions of genes and the relationship between the protein structure and its function. Here, we describe the analysis of lymphotoxin alpha expression in human retina and the generation of expression vectors to functional characterization of polymorphisms in the tumor necrosis factor locus using pCEFL-Flag expression vector and transfection assays in COS-1 cell line.

Published
2021

3. Analysis of Lymphotoxin Alpha Expression in Human Retina and Generation of Expression Vectors to Functional Characterization of Polymorphisms in the Tumor Necrosis Factor Locus

Author

Ricardo, Usategui-Martín, Irene, Rodriguez-Hernández, Rogelio, González-Sarmiento, Eva M, Sobas, Jose Carlos, Pastor-Jimeno, and Salvador, Pastor-Idoate

Subjects
Polymorphism, Genetic, Genetic Loci, Multigene Family, Gene Order, Genetic Vectors, Tumor Necrosis Factors, Gene Expression, Humans, Lymphotoxin-alpha, Retina
Abstract

With the evolution of new genomic sequencing technologies an important amount of genomic data has been provided. As a consequence of this, many gene polymorphisms have been shown to be significantly associated with different disorders. Many strategies have been implemented to reveal the role of having more than one allele at a specific locus and their involvement in the illnesses. Site-directed mutagenesis is one of the most common strategies to understand the regulatory regions of genes and the relationship between the protein structure and its function. Here, we describe the analysis of lymphotoxin alpha expression in human retina and the generation of expression vectors to functional characterization of polymorphisms in the tumor necrosis factor locus using pCEFL-Flag expression vector and transfection assays in COS-1 cell line.

Published
2020

4. OCT - Applications in Ophthalmology

Author

Salvador Pastor Idoate, Jose-Carlos Pastor Jimeno, Salvatore Di Lauro, Magdy Moussa, Mahmoud Leila, Jorge Novo, Joaquim De Moura, Manuel González Penedo, Marcos Ortega, Noelia Barreira, José Rouco, Xiaogang Wang, Jing Dong, Suhua Zhang, Bin Sun, Jorge Luis Domene, Jorge Luis Domene Hickman, Nuria Judith Aleman Hurtado, Timo Eppig, Achim Langenbucher, Stephanie Mäurer, Loay Daas, Berthold Seitz, Nur Acar, Simona Delia Nicoara, Mehmet Cuneyt Ozmen, Hüseyin Baran Özdemir, Sana Tinwala, Atul Kumar, Aydin Yildiz, Michele Lanza, Pastor Idoate, Salvador, Pastor Jimeno, Jose-Carlo, Di Lauro, Salvatore, Moussa, Magdy, Leila, Mahmoud, Novo, Jorge, De Moura, Joaquim, González Penedo, Manuel, Ortega, Marco, Barreira, Noelia, Rouco, José, Wang, Xiaogang, Dong, Jing, Zhang, Suhua, Sun, Bin, Luis Domene, Jorge, Luis Domene Hickman, Jorge, Judith Aleman Hurtado, Nuria, Eppig, Timo, Langenbucher, Achim, Mäurer, Stephanie, Daas, Loay, Seitz, Berthold, Acar, Nur, Delia Nicoara, Simona, Cuneyt Ozmen, Mehmet, Baran Özdemir, Hüseyin, Tinwala, Sana, Kumar, Atul, Yildiz, Aydin, and Lanza, Michele

Subjects
medicine.medical_specialty, business.industry, Ophthalmology, medicine, business, Ophthalmic pathology
Published
2018
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