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4 results on '"Joseane C. Ferreira"'

1. Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli

Author

Nathália Abichabki, Luísa V. Zacharias, Natália C. Moreira, Fernando Bellissimo-Rodrigues, Fernanda L. Moreira, Jhohann R. L. Benzi, Tânia M. C. Ogasawara, Joseane C. Ferreira, Camila M. Ribeiro, Fernando R. Pavan, Leonardo R. L. Pereira, Guilherme T. P. Brancini, Gilberto Ú. L. Braga, Antonio W. Zuardi, Jaime E. C. Hallak, José A. S. Crippa, Vera L. Lanchote, Rafael Cantón, Ana Lúcia C. Darini, and Leonardo N. Andrade

Subjects
Medicine, Science
Abstract

Abstract This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising.

Published
2022
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2. Cryptococcus neoformans var. grubii - Pathogenicity of environmental isolates correlated to virulence factors, susceptibility to fluconazole and molecular profile

Author

Reginaldo S Pedroso, Marco AS Lavrador, Joseane C Ferreira, Regina C Candido, and Claudia ML Maffei

Subjects
Cryptococcus neoformans, virulence factors, pathogenicity, molecular typing, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216
Abstract

The pathogenicity of Cryptococcus neoformans is heterogeneous and is associated with the expression of virulence factors. This study aimed to correlate the pathogenicity of C. neoformans var. grubii in BALB/c mice with in vitro virulence factors, fluconazole minimal inhibitory concentrations (MICs) and molecular profiles, before and after animal passage. Ten environmental isolates and one ATCC strain of C. neoformans var. grubii mating type α were evaluated. Most isolates (91%) killed 50% or more of the infected animals by day 24 postinfection and were recovered from the lungs and brains of surviving animals on days 7 and 14 postinfection. The burden of yeast in the lungs was more variable than that in the brain. The differences in the expression of virulence factors (growth at 37ºC, presence and size of the capsule and production of melanin, urease, proteinase and phospholipase) by most isolates pre and postpassage in animals were not statistically significant. The fluconazole MICs in postpassaged lines differed by a one-dilution from the MIC of the corresponding prepassaged line for six isolates. Using molecular typing [polymerase chain reaction-fingerprinting with (GACA)4 and M13], eight isolates were identified as VNI and three as VNII. We concluded that different isolates with the same molecular and phenotypic profiles, including isolates that are markedly hypervirulent, span a wide range of virulence and there were no changes in virulence factors in the postpassaged lines when compared with the corresponding nonpassaged lines.

Published
2010
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3. Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli

Author

Nathália, Abichabki, Luísa V, Zacharias, Natália C, Moreira, Fernando, Bellissimo-Rodrigues, Fernanda L, Moreira, Jhohann R L, Benzi, Tânia M C, Ogasawara, Joseane C, Ferreira, Camila M, Ribeiro, Fernando R, Pavan, Leonardo R L, Pereira, Guilherme T P, Brancini, Gilberto Ú L, Braga, Antonio W, Zuardi, Jaime E C, Hallak, José A S, Crippa, Vera L, Lanchote, Rafael, Cantón, Ana Lúcia C, Darini, and Leonardo N, Andrade

Subjects
Klebsiella pneumoniae, Drug Resistance, Multiple, Bacterial, Gram-Negative Bacteria, Drug Repositioning, Cannabidiol, Drug Synergism, Microbial Sensitivity Tests, Anti-Bacterial Agents, Polymyxin B
Abstract

This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising.

Published
2021

4. Leukotriene B

Author

Morgana K B, Prado, Gisele A, Locachevic, Karina F, Zoccal, Francisco W G, Paula-Silva, Caroline, Fontanari, Joseane C, Ferreira, Priscilla A T, Pereira, Luiz G, Gardinassi, Simone G, Ramos, Carlos A, Sorgi, Ana Lúcia C, Darini, and Lúcia H, Faccioli

Subjects
Inflammation, Mice, Knockout, alpha-Defensins, Mice, 129 Strain, 5-Lipoxygenase-Activating Proteins, Receptors, Leukotriene B4, hemic and immune systems, respiratory system, Leukotriene B4, Bacterial Load, Dinoprostone, Article, respiratory tract diseases, Mice, Phagocytosis, Achromobacter denitrificans, Macrophages, Alveolar, Animals, lipids (amino acids, peptides, and proteins), Gram-Negative Bacterial Infections, Lung, Cells, Cultured, Signal Transduction
Abstract

Leukotriene B4 (LTB4) is essential for host immune defence. It increases neutrophil recruitment, phagocytosis and pathogen clearance, and decreases oedema and inflammasome activation. The host response and the role of LTB4 during Achromobacter xylosoxidans infection remain unexplored. Wild-type (129sv) and LTB4 deficient (Alox5 −/−) mice were intratracheally infected with A. xylosoxidans. Wild-type 129sv infected mice survived beyond the 8th day post-infection, exhibited increased levels of LTB4 in the lung on the 1st day, while levels of PGE2 increased on the 7th day post-infection. Infected Alox5 −/− mice showed impaired bacterial clearance, increased lung inflammation, and succumbed to the infection by the 7th day. We found that exogenous LTB4 does not affect the phagocytosis of A. xylosoxidans by alveolar macrophages in vitro. However, treatment of infected animals with LTB4 protected from mortality, by reducing the bacterial load and inflammation via BLT1 signalling, the high affinity receptor for LTB4. Of importance, we uncovered that LTB4 induces gene and protein expression of α-defensin-1 during the infection. This molecule is essential for bacterial clearance and exhibits potent antimicrobial activity by disrupting A. xylosoxidans cell wall. Taken together, our data demonstrate a major role for LTB4 on the control of A. xylosoxidans infection.

Published
2016

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