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Your search keyword '"Jostina R. Rakoma"' showing total 4 results
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4 results on '"Jostina R. Rakoma"'

1. IMA genome - F14

Author

Magriet A. van der Nest, Renato Chávez, Lieschen De Vos, Tuan A. Duong, Carlos Gil-Durán, Maria Alves Ferreira, Frances A. Lane, Gloria Levicán, Quentin C. Santana, Emma T. Steenkamp, Hiroyuki Suzuki, Mario Tello, Jostina R. Rakoma, Inmaculada Vaca, Natalia Valdés, P. Markus Wilken, Michael J. Wingfield, and Brenda D. Wingfield

Subjects
Fusarium fujikuroi species complex (FFSC), Colombia, Pinus tecunumanii, Eucalyptus leaf pathogen, Botany, QK1-989
Abstract

Abstract Draft genomes of Penicillium roqueforti, Fusarium sororula, Chalaropsis populi, and Chrysoporthe puriensis are presented. Penicillium roqueforti is a model fungus for genetics, physiological and metabolic studies, as well as for biotechnological applications. Fusarium sororula and Chrysoporthe puriensis are important tree pathogens, and Chalaropsis populi is a soil-borne root-pathogen. The genome sequences presented here thus contribute towards a better understanding of both the pathogenicity and biotechnological potential of these species.

Published
2021
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2. Calpurnia aurea (Aiton) Benth Extracts Reduce Quorum Sensing Controlled Virulence Factors in Pseudomonas aeruginosa

Author

Sekelwa Cosa, Jostina R. Rakoma, Abdullahi A. Yusuf, and Thilivhali E. Tshikalange

Subjects
antipathogenic, anti-quorum sensing, biofilm formation, cell-to-cell communication, plant extract, molecular docking, Organic chemistry, QD241-441
Abstract

Pseudomonas aeruginosa is the causative agent of several life-threatening human infections. Like many other pathogens, P. aeruginosa exhibits quorum sensing (QS) controlled virulence factors such as biofilm during disease progression, complicating treatment with conventional antibiotics. Thus, impeding the pathogen’s QS circuit appears as a promising alternative strategy to overcome pseudomonas infections. In the present study, Calpurnia aurea were evaluated for their antibacterial (minimum inhibitory concentrations (MIC)), anti-quorum sensing/antivirulence (AQS), and antibiofilm potential against P. aeruginosa. AQS and antivirulence (biofilm formation, swimming, and swarming motility) activities of plant extracts were evaluated against Chromobacterium violaceum and P. aeruginosa, respectively. The in vitro AQS potential of the individual compounds were validated using in silico molecular docking. Acetone and ethanolic extracts of C. aurea showed MIC at 1.56 mg/mL. The quantitative violacein inhibition (AQS) assay showed ethyl acetate extracts as the most potent at a concentration of 1 mg/mL. GCMS analysis of C. aurea revealed 17 compounds; four (pentadecanol, dimethyl terephthalate, terephthalic acid, and methyl mannose) showed potential AQS through molecular docking against the CviR protein of C. violaceum. Biofilm of P. aeruginosa was significantly inhibited by ≥60% using 1-mg/mL extract of C. aurea. Confocal laser scanning microscopy correlated the findings of crystal violet assay with the extracts significantly altering the swimming motility. C. aurea extracts reduced the virulence of pseudomonas, albeit in a strain- and extract-specific manner, showing their suitability for the identification of lead compounds with QS inhibitory potential for the control of P. aeruginosa infections.

Published
2020
Full Text
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3. IMA genome - F14

Author

Carlos Gil-Durán, Natalia Valdés, Lieschen De Vos, Renato Chávez, Jostina R. Rakoma, P. Markus Wilken, Michael J. Wingfield, Frances A. Lane, Magriet A. van der Nest, Brenda D. Wingfield, Gloria Levicán, Quentin C. Santana, Mario Tello, Maria Alves Ferreira, Inmaculada Vaca, Emma Theodora Steenkamp, Tuan A. Duong, and Hiroyuki Suzuki

Subjects
0106 biological sciences, Fusarium, Genetics, 0303 health sciences, biology, Penicillium roqueforti, Fungus, Genome project, Armillaria root rot, biology.organism_classification, 01 natural sciences, Agricultural and Biological Sciences (miscellaneous), Genome, 03 medical and health sciences, Mycology, Chrysoporthe, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 010606 plant biology & botany
Abstract

Draft genomes of Penicillium roqueforti, Fusarium sororula, Chalaropsis populi, and Chrysoporthe puriensis are presented. Penicillium roqueforti is a model fungus for genetics, physiological and metabolic studies, as well as for biotechnological applications. Fusarium sororula and Chrysoporthe puriensis are important tree pathogens, and Chalaropsis populi is a soil-borne root-pathogen. The genome sequences presented here thus contribute towards a better understanding of both the pathogenicity and biotechnological potential of these species.

Published
2021
Full Text
View/download PDF

4. Calpurnia aurea (Aiton) Benth Extracts Reduce Quorum Sensing Controlled Virulence Factors in Pseudomonas aeruginosa

Author

Jostina R. Rakoma, Abdullahi Ahmed Yusuf, Sekelwa Cosa, and Thilivhali Emmanuel Tshikalange

Subjects
Pharmaceutical Science, Swarming motility, Virulence, anti-quorum sensing, medicine.disease_cause, Article, Analytical Chemistry, Microbiology, lcsh:QD241-441, 03 medical and health sciences, lcsh:Organic chemistry, Drug Discovery, medicine, Physical and Theoretical Chemistry, 030304 developmental biology, Calpurnia aurea, 0303 health sciences, biology, 030306 microbiology, Pseudomonas aeruginosa, Chemistry, Organic Chemistry, Pseudomonas, Biofilm, cell-to-cell communication, molecular docking, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Quorum sensing, antipathogenic, Chemistry (miscellaneous), biofilm formation, plant extract, Molecular Medicine, Chromobacterium violaceum
Abstract

Pseudomonas aeruginosa is the causative agent of several life-threatening human infections. Like many other pathogens, P. aeruginosa exhibits quorum sensing (QS) controlled virulence factors such as biofilm during disease progression, complicating treatment with conventional antibiotics. Thus, impeding the pathogen&rsquo, s QS circuit appears as a promising alternative strategy to overcome pseudomonas infections. In the present study, Calpurnia aurea were evaluated for their antibacterial (minimum inhibitory concentrations (MIC)), anti-quorum sensing/antivirulence (AQS), and antibiofilm potential against P. aeruginosa. AQS and antivirulence (biofilm formation, swimming, and swarming motility) activities of plant extracts were evaluated against Chromobacterium violaceum and P. aeruginosa, respectively. The in vitro AQS potential of the individual compounds were validated using in silico molecular docking. Acetone and ethanolic extracts of C. aurea showed MIC at 1.56 mg/mL. The quantitative violacein inhibition (AQS) assay showed ethyl acetate extracts as the most potent at a concentration of 1 mg/mL. GCMS analysis of C. aurea revealed 17 compounds, four (pentadecanol, dimethyl terephthalate, terephthalic acid, and methyl mannose) showed potential AQS through molecular docking against the CviR protein of C. violaceum. Biofilm of P. aeruginosa was significantly inhibited by &ge, 60% using 1-mg/mL extract of C. aurea. Confocal laser scanning microscopy correlated the findings of crystal violet assay with the extracts significantly altering the swimming motility. C. aurea extracts reduced the virulence of pseudomonas, albeit in a strain- and extract-specific manner, showing their suitability for the identification of lead compounds with QS inhibitory potential for the control of P. aeruginosa infections.

Published
2020

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