Search

Your search keyword '"Jouan, Véronique"' showing total 46 results
Start Over Author "Jouan, Véronique"
46 results on '"Jouan, Véronique"'

2. Identification of a viral gene essential for the genome replication of a domesticated endogenous virus in ichneumonid parasitoid wasps

Author

Lorenzi, Ange, primary, Legeai, Fabrice, additional, Jouan, Véronique, additional, Girard, Pierre-Alain, additional, Strand, Mickael, additional, Ravallec, Marc, additional, Eychenne, Magali, additional, Bretaudeau, Anthony, additional, Robin, Stéphanie, additional, Rochefort, Jeanne, additional, Villegas, Mathilde, additional, Burke, Gaelen, additional, Rebollo, Rita, additional, Nègre, Nicolas, additional, and Volkoff, Anne-Nathalie, additional

Published
2024
Full Text
View/download PDF

5. Identification of a viral gene essential for the genome replication of a domesticated endogenous virus in ichneumonid parasitoid wasps.

Author

Lorenzi, Ange, Legeai, Fabrice, Jouan, Véronique, Girard, Pierre-Alain, Strand, Michael R., Ravallec, Marc, Eychenne, Magali, Bretaudeau, Anthony, Robin, Stéphanie, Rochefort, Jeanne, Villegas, Mathilde, Burke, Gaelen R., Rebollo, Rita, Nègre, Nicolas, and Volkoff, Anne-Nathalie

Subjects
VIRAL genes, GLYCOCALYX, INSECT eggs, WASPS, RNA interference, GENOMES, VIRAL genomes
Abstract

Thousands of endoparasitoid wasp species in the families Braconidae and Ichneumonidae harbor "domesticated endogenous viruses" (DEVs) in their genomes. This study focuses on ichneumonid DEVs, named ichnoviruses (IVs). Large quantities of DNA-containing IV virions are produced in ovary calyx cells during the pupal and adult stages of female wasps. Females parasitize host insects by injecting eggs and virions into the body cavity. After injection, virions rapidly infect host cells which is followed by expression of IV genes that promote the successful development of wasp offspring. IV genomes consist of two components: proviral segment loci that serve as templates for circular dsDNAs that are packaged into capsids, and genes from an ancestral virus that produce virions. In this study, we generated a chromosome-scale genome assembly for Hyposotor didymator that harbors H. didymator ichnovirus (HdIV). We identified a total of 67 HdIV loci that are amplified in calyx cells during the wasp pupal stage. We then focused on an HdIV gene, U16, which is transcribed in calyx cells during the initial stages of replication. Sequence analysis indicated that U16 contains a conserved domain in primases from select other viruses. Knockdown of U16 by RNA interference inhibited virion morphogenesis in calyx cells. Genome-wide analysis indicated U16 knockdown also inhibited amplification of HdIV loci in calyx cells. Altogether, our results identified several previously unknown HdIV loci, demonstrated that all HdIV loci are amplified in calyx cells during the pupal stage, and showed that U16 is required for amplification and virion morphogenesis. Author summary: Parasitoid "domesticated endogenous viruses" (DEVs) provide a fascinating example of eukaryotes acquiring new functions through integration of a viral genome. DEVs consist of multiple loci in the genomes of wasps. Upon activation, these elements collectively orchestrate the production of virions or virus-like particles that are crucial for successful parasitism of host insects. Despite the significance of DEVs for parasitoid biology, the mechanisms regulating key steps in virion morphogenesis are largely unknown. In this study, we focused on the ichneumonid parasitoid Hyposoter didymator, which harbors an ichnovirus consisting of 67 proviral loci. Our findings reveal that all proviral loci are simultaneously amplified in ovary calyx cells of female wasps during the pupal stage suggesting the hijacking of cellular replication complexes by viral proteins. We functionally studied the ichnovirus gene U16, which encodes a protein with a weakly conserved primase C-terminal domain. Silencing U16 inhibited viral DNA amplification and virion production, underscoring the key role of this gene for ichnovirus replication. Our study provides evidence that many genes involved in viral DNA replication have been conserved during the domestication of ichnoviruses. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

10. Venom gland extract is not required for successful parasitism in the polydnavirus-associated endoparasitoid Hyposoter didymator (Hym. Ichneumonidae) despite the presence of numerous novel and conserved venom proteins

Author

Dorémus, Tristan, Urbach, Serge, Jouan, Véronique, Cousserans, François, Ravallec, Marc, Demettre, Edith, Wajnberg, Eric, Poulain, Julie, Azéma-Dossat, Carole, Darboux, Isabelle, Escoubas, Jean-Michel, Colinet, Dominique, Gatti, Jean-Luc, Poirié, Marylène, and Volkoff, Anne-Nathalie

Published
2013
Full Text
View/download PDF

14. Additional file 8 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 8: Table S9. Transposable elements (TE) found in Hyposter didymator segments, IVSPERs and neighboring regions. The LOLA package [86] was used to assess if some particular TE were enriched close to viral circles or IVSPER. Genomics positions were enlarged to 10 kbp at each segments ends and sampled against 1000 other similar regions from the genome, then used it a random reference. LOLA identifies overlaps and calculates enrichment for each TE. For each pairwise comparison, a series of columns describe the results of the statistical test (pvalueLog: -log10(pvalue) from the fisher’s exact result; oddsRatio: result from the fisher’s exact test; q-value transformation to provide false discovery rate (FDR) scores automatically). Some TE are enriched around viral locations, but after FDR correction, nothing was significant.

Published
2020
Full Text
View/download PDF

15. Additional file 10 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 10:. DRJs analysis. Figure S3. Examples of the different types of DRJ position. a. Proviral segment with two copies of a single direct repeat (DRJ1L and DRJ1R), one at each end of the segment. b. Proviral segment with two distinct repeated sequences (DRJ1, in yellow and DRJ2, in green), each present in two copies (DRJ1L and DRJ1R, DRJ2L and DRJ2R). c. Proviral segment with two repeated sequences, each present in two or more copies. DRJ1s in yellow, DRJ2s in green, HdIV genes represented by arrows. Table S11. DNA motifs found in the direct repeated sequences flanking the IV segments inserted in wasp genomes. Analysis was performed using the DNAMINDA2 webserver ( http://bmbl.sdstate.edu/DMINDA2/annotate.php ); the input dataset was composed of 99 DRJ sequences (right junctions of HdIV and CsIV segments). A total of 89 motifs were obtained; only those whose occurrence exceed 70% of the DRJs are reported. Table S12. Result of genome search using motifs predicted with DMINDA 2.0 webserver. Occurrence rate of motifs predicted with DMINDA 2.0 webserver in DRJs and whole genome sequences. Each of the two motifs was search among the 6 bp kmers present in the whole genome (201,969,604) and in the DRJs (33,930). The significance was evaluated using a Chi2 (taking into account the ratio of these motifs / all the other motifs in the DRJS and in the genome).

Published
2020
Full Text
View/download PDF

16. Additional file 9 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 9: Table S10. List of direct repeat junctions (DRJ) found at the ends or within proviral segments genes identified in Hyposoter didymator and Campoletis sonorensis genome scaffolds. Are indicated the scaffold name, the name of the proviral segment, its length and position in the scaffold, the name of the DRJ, its size and position in the scaffold and the DRJ sequence. Nucleotide identities are indicated for each pair of DRJ.

Published
2020
Full Text
View/download PDF

17. Additional file 6 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 6: Figure S1. H. didymator proviral loci corresponding to two segments previously described as “distinct” but sharing part of their sequence [36]. Segments Hd2a (GenBank: KJ586332.1) and Hd2b (GenBank: KJ586327.1) co-localize in the same genome locus here named Hd2; segments Hd11a (KJ586322.1) and Hd11b (KJ586302.1) co-localize in the same genome locus here named Hd11; Hd17a (KJ586314.1) and Hd17b (KJ586316.1) co-localize in the same genome locus here named Hd17; Hd20a (KJ586312.1) and Hd20b (KJ586297.1) co-localize in the same genome locus here named Hd20; Hd26a (KJ586301.1) and Hd26b (KJ586306.1) co-localize in the same genome locus here named Hd26; and finally, Hd31 (KJ586299.1) and Hd34 (KJ586295.1) co-localize in the same genome locus here named Hd31-34. Each proviral locus was characterized by the presence of two different direct repeated sequences (DRJ1 and DRJ2) at the extremities of each of the overlapping segments. Scale bar: 1000 nt.

Published
2020
Full Text
View/download PDF

18. Additional file 5 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 5: Table S7. List of ichnoviral genes identified in Hyposoter didymator and Campoletis sonorensis genome scaffolds containing at least on ichnovirus sequence. Are indicated the scaffold name, the name of the proviral segment or of the Ichnovirus structural protein encoding region (IVSPER) found in the scaffold, its length and position in the scaffold, the name of the gene, its position in the scaffold, if it contains or not introns, the size of the predicted protein, then the NCBI blast P search results (NCBI accession number and ID of the best match, the blast P e-value and the percentage of identities). Last column indicates comments, or notes reporting discrepancies in the genomic sequence compared with the original CDS sequence in NCBI database.

Published
2020
Full Text
View/download PDF

19. Additional file 4 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 4: Table S6. List of scaffolds in Hyposoter didymator and Campoletis sonorensis genomes containing at least on ichnovirus sequence. Are indicated the scaffold name and length, the name of the proviral segment or of the Ichnovirus structural protein encoding region (IVSPER) found in the scaffold, its length and position in the scaffold, the name of the direct repeats flanking the segment or within the segment, and the name of the genes predicted in each viral locus. DRJ, direct repeat junction; R, right; L, left; int, internal.

Published
2020
Full Text
View/download PDF

20. Additional file 2 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 2:. Orthogroups analyses. Table S2. Orthofinder clustering metrics. G50: cluster size at which 50% of genes are in an orthogroup (OG) of that size or greater. O50: fewest number of orthogroups required to reach G50; G50 (assigned genes) = 16; G50 (all genes) = 14; O50 (assigned genes) = 3063; O50 (all genes) = 4112. Species carrying a PDV are indicated with an asterisk. Species carrying polydnaviruses are indicated by asterisks. Table S3. Number of orthogroups shared by each species-pair (i.e. the number of orthogroups which contain at least one gene from each of the species-pairs). Species carrying a PDV are indicated with an asterisk. Table S4. Number of species-specific orthogroups. Number of orthogroups specific to one or groups of species.

Published
2020
Full Text
View/download PDF

21. Additional file 3 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Abstract

Additional file 3:. Table S5. Synteny blocks between pairwise comparisons of multiple parasitoid genomes. Synteny blocks were computed using SynChro [89], a tool based on a simple algorithm that computes Reciprocal Best-Hits (RBH) to reconstruct the backbones of the synteny blocks. Species carrying polydnaviruses are indicated by asterisks. [Ichn.]: Ichneumonid; [Braco.]: Braconid.

Published
2020
Full Text
View/download PDF

22. The virus particles of Bathyplectes spp., parasitoids of the alfalfa weevil, are related to ichnoviruses

Author

Robin, Stéphanie, Ravallec, Marc, Frayssinet, Marie, Whitfield, James B., Jouan, Véronique, Legeai, Fabrice, Volkoff, Anne Nathalie, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Dept Entomology, Illinois State University, Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
ichnovirus, charancon, education, fungi, food and beverages, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, luzerne, alfalfa, health care economics and organizations
Abstract

The virus particles of [i]Bathyplectes[/i] spp., parasitoids of the alfalfa weevil, are related to ichnoviruses. 6. International Entomophagous Insects Conference

Published
2019

23. Decoding morphogenesis of Ichnovirus associated to the parasitic wasp H. didymator by RNA interference

Author

Lorenzi, Ange, Jouan, Véronique, Ravallec, Marc, Eychenne, Magali, Volkoff, Anne Nathalie, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), and Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)

Subjects
rna interference, morphogénèse virale, gène viral, macromolecular substances, guêpe, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, viral genes, arn interférence
Abstract

Decoding morphogenesis of Ichnovirus associated to the parasitic wasp [i]H. didymator[/i] by RNA interference. SIP/IOBC

Published
2019

24. Allaiter : Pourquoi ? Comment ? : idées reçues sur l'allaitement maternel Ed. 2

Author

Boulinguez-Jouan, Véronique, Boulinguez-Jouan, Véronique, Boulinguez-Jouan, Véronique, and Boulinguez-Jouan, Véronique

Abstract

Allaiter ou donner le biberon ? Toute femme enceinte est confrontée à ce choix. Évident pour certaines, il est compliqué pour d’autres d’autant que les idées reçues sont légion : lait maternel pas assez nourrissant, pratique douloureuse, sevrage difficile, etc. Exposé à l’influence des différentes cultures, aux désaccords entre professionnels et aux préjugés des mères, l’allaitement gagne à être expliqué afin de démêler le vrai du faux.C’est l’objectif de cet ouvrage dans lequel Véronique Boulinguez-Jouan s’efforce de répondre aux questions qui lui sont le plus fréquemment posées. Véronique Boulinguez-Jouan est sage-femme puéricultrice, consultante en lactation IBCLC. Après avoir exercé pendant 12 ans à la maternité de Port Royal où elle était référente pour l’allaitement maternel, elle travaille actuellement à la PMI de Paris.

Published
2017

25. Western European populations of the ichneumonid wasp Hyposoter didymator belong to a single taxon

Author

Frayssinet, Marie, Audiot, Philippe, Cusumano, Antonino, Pichon, Apolline, Malm, Lisa, Jouan, Véronique, Vabre, Marin, Malavieille, Serge, Delalande, Magalie, Vargas-Osuna, Enrique, Bourguet, Denis, and Volkoff, Anne Nathalie

Subjects
Biodiversity and Ecology, parasitoïde, Biodiversité et Ecologie, hyposoter didymator, fungi, Hyposoter didymator, Helicoverpa amigera, population genetic structure, host preferences, western European populations, analyse génétique
Abstract

Hyposoter didymator (Hymenoptera, Ichneumonidae) is a generalist solitary endoparasitoid of noctuid larvae. In the present work, we tested whether populations of H. didymator were divided in several genetically distinct taxa as described for many other generalist parasitoid species, and whether differences in H. didymator parasitism rates were explained by the insect host species and/or by the plant on which these hosts were feeding on. The genetic analysis of natural populations collected in different regions in France and Spain on seven different insect hosts and seven different host plants (775 individuals) showed that H. didymator populations belong to a unique single taxon. However, H. didymator seems to be somewhat specialized. Indeed, in the fields it more often parasitized Helicoverpa armigera compared to the other host species collected in the present work. Also, H. didymator parasitism rates in field conditions and semi-field experimental studies were dependent on the host plants on which H. armigera larvae are feeding. Still, H. didymator can occur occasionally on non-preferred noctuid species. One hypothesis explaining the ability of H. didymator to switch hosts in natura could be related to fluctuating densities of the preferred host over the year; this strategy would allow the parasitoid to avoid seasonal population collapses.

Published
2019

26. Recurrent virus domestication in parasitic wasps

Author

Volkoff, Anne-Nathalie, Drezen, Jean-Michel, Leobold, Matthieu, Bezier, Annie, Jouan, Véronique, Herniou, Elisabeth, Huguet, Elisabeth, Ravallec, Marc, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Francois Rabelais [Tours], and Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
insecte hôte, écologie bactérienne, parasite, virus, Rhopalocera, lepidoptera, guêpe, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Published
2018

27. RNA interference identifies domesticated viral genes involved in assembly and trafficking of virus-derived particles in ichneumonid wasps

Author

Lorenzi, Ange, primary, Ravallec, Marc, additional, Eychenne, Magali, additional, Jouan, Véronique, additional, Robin, Stéphanie, additional, Darboux, Isabelle, additional, Legeai, Fabrice, additional, Gosselin-Grenet, Anne-Sophie, additional, Sicard, Mathieu, additional, Stoltz, Don, additional, and Volkoff, Anne-Nathalie, additional

Published
2019
Full Text
View/download PDF

28. Conserved and specific genomic features of endogenous polydnaviruses revealed by whole genome sequencing of two ichneumonid wasps

Author

Legeai, Fabrice, primary, Santos, Bernardo F., additional, Robin, Stéphanie, additional, Bretaudeau, Anthony, additional, Dikow, Rebecca B., additional, Lemaitre, Claire, additional, Jouan, Véronique, additional, Ravallec, Marc, additional, Drezen, Jean-Michel, additional, Tagu, Denis, additional, Gyapay, Gabor, additional, Zhou, Xin, additional, Liu, Shanlin, additional, Webb, Bruce A., additional, Brady, Seán G., additional, and Volkoff, Anne-Nathalie, additional

Published
2019
Full Text
View/download PDF

29. Western European Populations of the Ichneumonid Wasp Hyposoter didymator Belong to a Single Taxon

Author

Frayssinet, Marie, primary, Audiot, Philippe, additional, Cusumano, Antonino, additional, Pichon, Apolline, additional, Malm, Lisa E., additional, Jouan, Véronique, additional, Vabre, Marin, additional, Malavieille, Serge, additional, Delalande, Magalie, additional, Vargas-Osuna, Enrique, additional, Bourguet, Denis, additional, and Volkoff, Anne-Nathalie, additional

Published
2019
Full Text
View/download PDF

30. Recurrent domestication of viral genomes leads to different virulence strategies in ichneumonid wasps

Author

Aury, Jean Marc, Barbe, Valérie, Bezier, Annie, Drezen, Jean-Michel, Jouan, Véronique, Legeai, Fabrice, Li, L., Pichon, Apolline, Ravallec, Marc, Urbach, Serge, Volkoff, Anne-Nathalie, Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Escola Super Agr Luiz Queiroz, Dept Entomol & Acarol, Lab Interacoes Insetos, Universidade de São Paulo (USP), Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Francois Rabelais [Tours], Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, China National GeneBank, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Universidade de São Paulo = University of São Paulo (USP), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
virulence, ichnovirus, guêpe, lutte biologique, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Published
2016

31. Origine évolutive des 'Virus-like particles' de Venturia canescens

Author

Pichon, Apolline, Urbach, Serge, Aury, Jean Marc, Bezier, Annie, Jouan, Véronique, Ravallec, Marc, Wurmser, François, Guy, Julie, Barbe, Valérie, Cousserans, Francois, Gauthier, Jérémy, Demettre, Edith, Sibut, Vonnick, Drezen, Jean-Michel, Volkoff, Anne-Nathalie, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), PPF Analyse des systèmes biologiques, Université Francois Rabelais [Tours], Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
venturia canescens, guêpe, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Published
2014

32. Recurrent domestication of viruses by parasitic wasps to face host immune defenses

Author

Drezen, Jean-Michel, Bezier, Annie, Pichon, Apolline, Jouan, Véronique, Périquet, Georges, Gyapay, Gabor, Barbe, Valérie, Herniou, Elisabeth A., Huguet, Elisabeth, Volkoff, Anne-Nathalie, ProdInra, Archive Ouverte, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de Génomique d'Evry (IG), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Faculté des Sciences et Techniques, Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Francois Rabelais [Tours], Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDE.BE] Environmental Sciences/Biodiversity and Ecology, hôte, parasite, virus, guêpe, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, biodiversité
Published
2014

33. Acquisition of viruses as a driver in endoparasitic ichneumonid wasp evolution

Author

Pichon, Apolline, Urbach, Serge, Bezier, Annie, Aury, Jean Marc, Jouan, Véronique, Ravallec, Marc, Guy, Julie, Cousserans, Francois, FRAYSSINET, Marie, Colinet, Dominique, Barbe, Valérie, Drezen, Jean-Michel, Volkoff, Anne-Nathalie, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Faculté des Sciences et Techniques, Institut de Recherche sur la Biologie de l’Insecte, UMR CNRS 7261, Université Francois Rabelais [Tours], Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
infection virale, virus, guêpe, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, écologie des populations, biodiversité
Published
2014

34. Recurrent DNA virus domestication leading to different parasite virulence strategies

Author

Pichon, Apolline, primary, Bézier, Annie, additional, Urbach, Serge, additional, Aury, Jean-Marc, additional, Jouan, Véronique, additional, Ravallec, Marc, additional, Guy, Julie, additional, Cousserans, François, additional, Thézé, Julien, additional, Gauthier, Jérémy, additional, Demettre, Edith, additional, Schmieder, Sandra, additional, Wurmser, François, additional, Sibut, Vonick, additional, Poirié, Marylène, additional, Colinet, Dominique, additional, da Silva, Corinne, additional, Couloux, Arnaud, additional, Barbe, Valérie, additional, Drezen, Jean-Michel, additional, and Volkoff, Anne-Nathalie, additional

Published
2015
Full Text
View/download PDF

36. Corrigendum to “Venom gland extract is not required for successful parasitism in the polydnavirus-associated endoparasitoid Hyposoter didymator (Hym. Ichneumonidae) despite the presence of numerous novel and conserved venom proteins” [Insect Biochem. Mol. Biol. 43 (2013), 292–307]

Author

Dorémus, Tristan, primary, Urbach, Serge, additional, Jouan, Véronique, additional, Cousserans, François, additional, Ravallec, Marc, additional, Demettre, Edith, additional, Wajnberg, Eric, additional, Poulain, Julie, additional, Azéma-Dossat, Carole, additional, Darboux, Isabelle, additional, Escoubas, Jean-Michel, additional, Colinet, Dominique, additional, Gatti, Jean-Luc, additional, Poirié, Marylène, additional, and Volkoff, Anne-Nathalie, additional

Published
2014
Full Text
View/download PDF

39. Analysis of Virion Structural Components Reveals Vestiges of the Ancestral Ichnovirus Genome

Author

Volkoff, Anne-Nathalie, primary, Jouan, Véronique, additional, Urbach, Serge, additional, Samain, Sylvie, additional, Bergoin, Max, additional, Wincker, Patrick, additional, Demettre, Edith, additional, Cousserans, François, additional, Provost, Bertille, additional, Coulibaly, Fasseli, additional, Legeai, Fabrice, additional, Béliveau, Catherine, additional, Cusson, Michel, additional, Gyapay, Gabor, additional, and Drezen, Jean-Michel, additional

Published
2010
Full Text
View/download PDF

40. Extensive Transcription Analysis of the Hyposoter didymator Ichnovirus Genome in Permissive and Non-Permissive Lepidopteran Host Species.

Author

Dorémus, Tristan, Cousserans, François, Gyapay, Gabor, Jouan, Véronique, Milano, Patricia, Wajnberg, Eric, Darboux, Isabelle, Cônsoli, Fernando Luis, and Volkoff, Anne-Nathalie

Subjects
GENETIC transcription, VIRAL genomes, CATERPILLARS, DNA viruses, ENDOPARASITES, VIRUS virulence, VIRAL proteins, VIRUSES
Abstract

Ichnoviruses are large dsDNA viruses that belong to the Polydnaviridae family. They are specifically associated with endoparasitic wasps of the family Ichneumonidae and essential for host parasitization by these wasps. We sequenced the Hyposoter didymator Ichnovirus (HdIV) encapsidated genome for further analysis of the transcription pattern of the entire set of HdIV genes following the parasitization of four different lepidopteran host species. The HdIV genome was found to consist of at least 50 circular dsDNA molecules, carrying 135 genes, 98 of which formed 18 gene families. The HdIV genome had general features typical of Ichnovirus (IV) genomes and closely resembled that of the IV carried by Hyposoter fugitivus. Subsequent transcriptomic analysis with Illumina technology during the course of Spodoptera frugiperda parasitization led to the identification of a small subset of less than 30 genes with high RPKM values in permissive hosts, consisting with these genes encoding crucial virulence proteins. Comparisons of HdIV expression profiles between host species revealed differences in transcript levels for given HdIV genes between two permissive hosts, S. frugiperda and Pseudoplusia includens. However, we found no evident intrafamily gene-specific transcription pattern consistent with the presence of multigenic families within IV genomes reflecting an ability of the wasps concerned to exploit different host species. Interestingly, in two non-permissive hosts, Mamestra brassiccae and Anticarsia gemmatalis (most of the parasitoid eggs were eliminated by the host cellular immune response), HdIV genes were generally less strongly transcribed than in permissive hosts. This suggests that successful parasitism is dependent on the expression of given HdIV genes exceeding a particular threshold value. These results raise questions about the mecanisms involved in regulating IV gene expression according to the nature of the lepidopteran host species encountered. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

41. Additional file 11 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Subjects
3. Good health
Abstract

Additional file 11 Manual analysis of the DRJ regions containing an excision site. Alignments of Sanger-sequenced DRJ regions from integrated and circular forms of seven H. didymator IV segments containing a putative excision site.

42. Additional file 7 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Subjects
3. Good health
Abstract

Additional file 7: Dispersion of the viral loci within ichneumonid genomes. Table S8. Distance (in bp) between two segments, a segment and an IVSPER or between two IVSPERs localized in the same scaffold. Figure S2. Graphical representation of the mean distance (in Kbp) between viral loci in H. didymator and C. sonorensis genomes. Data are given between 2 segments, between a segment and an IVSPER, and/or between 2 IVSPERs.

43. Additional file 1 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Subjects
3. Good health
Abstract

Additional file 1: Table S1. Transposable elements (TE) in the genomes of Hyposoter didymator and Campoletis sonorensis. Total number and percentage are given for each TE class. Detection performed using the REPET pipeline (see Methods). LINE, long interspersed nuclear element; LTR, long terminal repeat; SINE, short interspersed nuclear element.

44. Additional file 11 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Subjects
3. Good health
Abstract

Additional file 11 Manual analysis of the DRJ regions containing an excision site. Alignments of Sanger-sequenced DRJ regions from integrated and circular forms of seven H. didymator IV segments containing a putative excision site.

45. Additional file 7 of Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps

Author

Legeai, Fabrice, Santos, Bernardo F., Robin, Stéphanie, Bretaudeau, Anthony, Dikow, Rebecca B., Lemaitre, Claire, Jouan, Véronique, Ravallec, Marc, Jean-Michel Drezen, Tagu, Denis, Baudat, Frédéric, Gabor Gyapay, Zhou, Xin, Shanlin Liu, Webb, Bruce A., Brady, Seán G., and Volkoff, Anne-Nathalie

Subjects
3. Good health
Abstract

Additional file 7: Dispersion of the viral loci within ichneumonid genomes. Table S8. Distance (in bp) between two segments, a segment and an IVSPER or between two IVSPERs localized in the same scaffold. Figure S2. Graphical representation of the mean distance (in Kbp) between viral loci in H. didymator and C. sonorensis genomes. Data are given between 2 segments, between a segment and an IVSPER, and/or between 2 IVSPERs.

46. First extensive characterization of the venom gland from an egg parasitoid: structure, transcriptome and functional role

Author

Fabrice Legeai, Véronique Jouan, Antonino Cusumano, Marc Ravallec, Ezio Peri, Bernard Duvic, Anne-Nathalie Volkoff, Stefano Colazza, Department of Agricultural, Food and Forest Sciences, Università degli studi di Palermo - University of Palermo, Laboratory of Entomology, School of agriculture, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), BioInformatics Platform for Agroecosystems Arthropods, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), EMBO [533 - 2012], European Project: 609398,EC:FP7:PEOPLE,FP7-PEOPLE-2013-COFUND,AGREENSKILLSPLUS(2014), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Cusumano, Antonino, Duvic, Bernard, Jouan, Véronique, Ravallec, Marc, Legeai, Fabrice, Peri, Ezio, Colazza, Stefano, Volkoff, Anne-Nathalie, Université de Rennes (UR), and Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST

Subjects
0106 biological sciences, 0301 basic medicine, Physiology, Glycosylases, Wasps, Venom, Laccases, Hymenoptera, Insect, melanization, 01 natural sciences, virulence factor, Parasitoid, Transcriptome, Physiological suppression, Laboratory of Entomology, Arthropod Venoms, media_common, Larva, biology, Virulence factors, Phenotype, Nezara viridula, laccazes, Insect Proteins, Female, Melanization, media_common.quotation_subject, Zoology, complex mixtures, Host-Parasite Interactions, Heteroptera, 03 medical and health sciences, glycosylases, Exocrine Glands, Microscopy, Electron, Transmission, Animals, Peptidase, Host (biology), Laccase, fungi, biology.organism_classification, Laboratorium voor Entomologie, physiological suppression, 010602 entomology, 030104 developmental biology, Settore AGR/11 - Entomologia Generale E Applicata, peptidases, Insect Science, EPS, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Peptidases, Glycosylase
Abstract

The venom gland is a ubiquitous organ in Hymenoptera. In insect parasitoids, the venom gland has been shown to have multiple functions including regulation of host immune response, host paralysis, host castration and developmental alteration. However, the role played by the venom gland has been mainly studied in parasitoids developing in larval or pupal hosts while little is known for parasitoids developing in insect eggs. We conducted the first extensive characterization of the venom of the endoparasitoid Ooencyrtus telenomicida (Vassiliev), a species that develops in eggs of the stink bug Nezara viridula (L.). In particular we investigated the structure of the venom apparatus, its functional role and conducted a transcriptomic analysis of the venom gland. We found that injection of O. telenomicida venom induces: 1) a melanized-like process in N. viridula host eggs (host-parasitoid interaction), 2) impairment of the larval development of the competitor Trissolcus basalis (Wollaston) (parasitoid-parasitoid interaction). The O. telenomicida venom gland transcriptome reveals a majority of digestive enzymes (peptidases and glycosylases) and oxidoreductases (laccases) among the most expressed genes. The former enzymes are likely to be involved in degradation of the host resources for the specific benefit of the O. telenomicida offspring. In turn, alteration of host resources caused by these enzymes may negatively affect the larval development of the competitor T. basalis. We hypothesize that the melanization process induced by venom injection could be related to the presence of laccases, which are multicopper oxidases that belong to the phenoloxidases group. This work contributed to a better understanding of the venom in insect parasitoids and allowed to identify candidate genes whose functional role can be investigated in future studies.

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results