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Strontium (Sr) ions were added to porous magnesium (Mg) oxide with silicon and fluorine by microarc oxidation (MAO) to improve its osteogenic and pro-angiogenic properties. First, pure Mg was oxidized by MAO, and Sr was added by electrolysis. The surface of the resulting Sr coating was characterized by SEM, EDS, and EDS mapping. The release of Sr ions was monitored by ICP-OES. The antibacterial property of the coating was assessed against Staphylococcus aureus. The effect of Sr coating on osteogenesis was tested in MC3T3-E1 cell line by performing cell adhesion and proliferation tests, alkaline phosphatase (ALP) activity detection, cell morphology characterization, alizarin red staining, and osteogenic-related gene expression analysis. Finally, HUVECs cells were used to test the effect of Sr coating on angiogenesis through cell migration and tube formation assays, VEGF quantification, chicken embryo chorioallantoic membranes (CAM) test, and angiogenic-related gene expression analysis. The results showed that Sr coating had a hierarchical microstructure with a microporous structure evenly covered with nano-grains and that the Sr elements from the coating were released slowly and continuously. Sr coating had effective antibacterial properties and promoted cell adhesion, proliferation, ALP release, calcium nodule formation, and upregulated osteogenic gene expression. Moreover, the coating could promote migration, tube formation, VEGF expression, and angiogenic gene upregulation in endothelial cells. Sr coating also enhanced angiogenesis of CAM. This study supports that Sr coating on Mg- MAO enhances osteogenesis and angiogenesis.

This study was designed to investigate the anti-inflammatory effects of volatile oil of Platycladus orientalis (L.) Franco leaves (VOPF) and the underlying molecular mechanisms by using the non-infectious inflammation rat models and infectious inflammation mouse models. Ear swelling and intraperitoneal capillary permeability in mice, and carrageenan-induced toe swelling and cotton ball-induced granuloma in rats were used to reveal anti-inflammatory effects of VOPF. Moreover, the lipopolysaccharide (LPS)-induced mouse model of acute lung injury was used to explore the anti-inflammatory mechanism of VOPF. The results showed that VOPF could significantly inhibit auricular swelling, intraperitoneal capillary permeability in mice, and reduce granuloma swelling and paw swelling in rats. Furthermore, it significantly alleviated the pathological damage of the lung tissue. In addition, VOPF could reduce the contents of IL-1β and TNF-α and increase the content of IL-10 in the serum. It had little effect on the expression of p65 but reduced the phosphorylation level of p65 and IκB in NF-κB pathway. In conclusion, VOPF has anti-inflammatory effects and the mechanisms involve the down-regulation of the phosphorylation levels of p65 and IκB and blockage of the NF-κB signaling pathway.

Objective: To analyze and discuss the rules and thoughts of Professor Dai Xiaohua's treatment of coronary heart disease and chronic heart failure using data mining technology. Methods: The medical records of outpatients from Professor Dai Xiaohua, who were treated in the Department of Cardiology, First Affiliated Hospital of Anhui University of Traditional Chinese Medicine from June 2019 to October 2019 and standardize it. With the help of Microsoft Excel 2016, SPSS 23.0, and SPSS Modeler 18.0 computer software, a database was established and the frequency of medication, association rules, and cluster analysis were performed. Results: Of the 109 prescriptions included in the study, 87 Chinese medicines were involved. The top 10 drugs are Chenpi, Atractylodes, Dandelion, Zhuru, Muxiang, Fructus aurantii, Ophiopogon, Poria, Sour Jujube, semen coicis. A total of 10 associations such as “Bamboo Ru → Dandelion, Dandelion → Cinnamon”; Cluster analysis yields 5 clustering square groups. Conclusion: Professor Dai Xiaohua's treatment of coronary heart disease and chronic heart failure focuses on regulating qi and strengthening spleen, resolving phlegm and dampness.

Micro-arc oxidation (MAO) was used to improve the resistance of pure magnesium (Mg). Copper (Cu), a good antibacterial, angiogenic, and osteogenic element, was added by reaction in a Cu-containing electrolyte to improve the osteogenic and pro-angiogenic activities of Mg. The surface microstructures of the resulting MAO were evaluated by a scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS) mapping. The release of Cu ions was detected by ICP-OES. The antibacterial activity of films with different concentrations of Cu ions was assessed against Staphylococcus aureus (S. aureus). The osteogenesis of films was confirmed by cell morphology and proliferation, ALP activity, alizarin red staining, and osteogenic-related gene expression in the MC3T3-E1 cell line. The angiogenesis of the films was tested in human umbilical vein endothelial cells (HUVECs) by cell migration, tube formation, and VEGF quantification in vitro, and by a chicken embryo chorioallantoic membrane (CAM) assay in vivo. The results showed that the microporous structure was shaped by MAO, and the Cu group was denser and more uniform. The Cu coating showed effective antibacterial activity against S. aureus while also enhancing osteogenesis and angiogenesis in vitro. According to the CAM assay, the Cu group showed not only biocompatibility but also a significant angiogenic response, which was consistent with in vitro studies. The findings indicate that a Cu coating on Mg-MAO enhances osteogenesis and angiogenesis.

Toxoplasma gondii is an important intracellular parasitic protozoan with a variety of hosts, including chickens, which poses a potential threat to public health. However, little is known regarding overall T. gondii infection in chickens in China. Herein, the prevalence and risk factors associated with T. gondii infection in chickens in China were investigated using a meta-analysis. Forty studies regarding the prevalence of T. gondii in chickens in China from 1993 to 2021 were identified using five databases (PubMed, Science Direct, CNKI, Wang Fang, and VIP). Quantitative and potential sources were analyzed through subgroup analysis and meta-regression in R v3.5.2. The overall prevalence of T. gondii in chickens in China was 13.4% (95% confidence interval (CI): 10.9-16.0). In the region subgroup, the lowest prevalence was presented in Northwestern China (6.0%, 95% CI: 3.2-9.5; P 0.001). Seasonally, T. gondii prevalence was the highest in spring (17.9%, 95% CI: 7.7-30.9; P = 0.007). Among detection methods, the prevalence in the ELISA subgroup was the highest (22.8%, 95% CI: 17.1-29.1; P 0.001). According to the farming mode, the prevalence of T. gondii in free-range chickens (19.5%, 95% CI: 15.4-23.9) was significantly higher than that in chickens raised by intensive farming (7.4%, 95% CI: 5.1-10.2; P 0.001). We also estimated the relationships between region, sampling year, chicken age, chicken application, gender, sample classification, study quality, and T. gondii prevalence in chickens in China. Our study showed that region, season, and farming model played important roles in T. gondii infection of chickens. Integrated control measures should be undertaken to reduce the losses caused by T. gondii infection to the chicken industry.

This study was designed to investigate the anti-inflammatory effects of volatile oil of Platycladus orientalis (L.) Franco leaves (VOPF) and the underlying molecular mechanisms by using the non-infectious inflammation rat models and infectious inflammation mouse models. Ear swelling and intraperitoneal capillary permeability in mice, and carrageenan-induced toe swelling and cotton ball-induced granuloma in rats were used to reveal anti-inflammatory effects of VOPF. Moreover, the lipopolysaccharide (LPS)-induced mouse model of acute lung injury was used to explore the anti-inflammatory mechanism of VOPF. The results showed that VOPF could significantly inhibit auricular swelling, intraperitoneal capillary permeability in mice, and reduce granuloma swelling and paw swelling in rats. Furthermore, it significantly alleviated the pathological damage of the lung tissue. In addition, VOPF could reduce the contents of IL-1β and TNF-α and increase the content of IL-10 in the serum. It had little effect on the expression of p65 but reduced the phosphorylation level of p65 and IκB in NF-κB pathway. In conclusion, VOPF has anti-inflammatory effects and the mechanisms involve the down-regulation of the phosphorylation levels of p65 and IκB and blockage of the NF-κB signaling pathway.

In the Qinghai-Tibet Plateau of China, the yak is an animal of particular economic interest, which provides protein and income for herders in daily life. Brucellosis is a bacterial disease that can infect humans and animals, including yaks. It can damage the yak reproductive system, causing miscarriage and orchitis. At the same time, brucellosis threatens the health of herders. We performed this meta-analysis using R software to explore the combined prevalence and risk factors of brucellosis in yak in China. Variability was assessed by the I2 statistic and Cochran Q statistic. We identified 52 publications of related research from four databases (Wanfang Data, VIP Chinese Journal Database, China National Knowledge Infrastructure, and of PubMed). The pooled prevalence of yak brucellosis was 8.39 %. Prevalence was highest in Southwestern China (11.1 %). The point estimate of brucellosis in yak from 2012 to 2016 was the highest (11.47 %). The point estimate of age ≤ 12 months (1.44 %) was lower than that of age > 12 months (15.6 %). This study shows that yak brucellosis is serious, and its incidence is higher than before 2012. We recommend carrying out large-scale yak brucellosis investigations in Western China and conducting comprehensive testing planning. The detection of brucellosis in adult animals should be strengthened to reduce the economic loss caused by brucellosis to herders and to improve public health.

Tomato is one of the most important vegetables in the world. Decay after harvest is a major issue in the development of tomato industry. Currently, the most effective method for controlling decay after harvest is storage of tomato at low temperature combined with usage of chemical bactericide; however, long-term usage of chemical bactericide not only causes pathogen resistance but also is harmful for human health and environment. Biocontrol method for the management of disease after tomato harvest has great practical significance. In this study, antagonistic bacterium B-6-1 strain was isolated from the surface of tomato and identified as Enterobacter cowanii based on morphological characteristics and physiological and biochemical features combined with sequence analysis of 16SrDNA and ropB gene and construction of dendrogram. Effects of different concentrations of antagonistic bacterium E. cowanii suspension on antifungal activity after tomato harvest were analyzed by mycelium growth rate method. Results revealed that antifungal activity was also enhanced with increasing concentrations of antagonistic bacterium; inhibitory rates of 1 × 105 colony-forming units (cfu)/mL antagonistic bacterial solution on Fusarium verticillioides, Alternaria tenuissima, and Botrytis cinerea were 46.31%, 67.48%, and 75.67%, respectively. By using in vivo inoculation method, it was further confirmed that antagonistic bacterium could effectively inhibit the occurrence of B. cinerae after tomato harvest, biocontrol effect of 1 × 109 cfu/mL zymotic fluid reached up to 95.24%, and antagonistic bacterium E. cowanii has biocontrol potential against B. cinerea after harvest of fruits and vegetables.

Background PEST-containing nuclear protein (PCNP), a novel nuclear protein, is involved in cell proliferation and tumorigenesis. However, the precise mechanism of action of PCNP in the process of tumor growth has not yet been fully elucidated. Methods ShRNA knockdown and overexpression of PCNP were performed in human neuroblastoma cells. Tumorigenic and metastatic effects of PCNP were examined by tumor growth, migration, and invasion assays in vitro, as well as xenograft tumor assay in vivo. Results PCNP over-expression decreased the proliferation, migration, and invasion of human neuroblastoma cells and down-regulation of PCNP showed reverse effects. PCNP over-expression increased protein expressions of cleaved caspase-3, cleaved caspase-8, cleaved caspase-9, and cleaved poly adenosine diphosphate-ribose polymerase, as well as ratios of B-cell lymphoma-2 (Bcl-2)-associated X protein/Bcl-2 and Bcl-2-associated death promoter/B-cell lymphoma-extra large in human neuroblastoma cells, however PCNP knockdown exhibited reverse trends. PCNP over-expression increased phosphorylations of extracellular signal-regulated protein kinase 1/2, p38, c-Jun N-terminal kinase, as well as decreased phosphorylations of phosphatidylinositol 3-kinase (PI3K), Akt, and mammalian target of rapamycin (mTOR), nevertheless PCNP knockdown exhibited opposite effects. Furthermore, PCNP over-expression significantly reduced the growth of human neuroblastoma xenograft tumors by down-regulating angiogenesis, whereas PCNP knockdown markedly promoted the growth of human neuroblastoma xenograft tumors through up-regulation of angiogenesis. Conclusions PCNP mediates the proliferation, migration, and invasion of human neuroblastoma cells through mitogen-activated protein kinase and PI3K/AKT/mTOR signaling pathways, implying that PCNP is a therapeutic target for patients with neuroblastoma.

The liver X receptors (LXRs) is an important component of the nuclear receptor (NR) superfamily. Previous studies have shown that the LXRs possessed antitumor activity in various types of tumor cells. However, the complicated mechanisms underlying the antitumor activity remain largely unexplored. In this study, we incubated A549 cells with the compound T0901317, a specific LXRs agonist, for 24 h. The MTT assay was used to assess cell viability. Transwell assays were used to evaluate cell migration and invasion. The shRNA was utilized for RNA interference. The target gene and protein expression levels were assessed using reverse transcription-PCR and western blot assay. The DNA-binding activity of nuclear factor κB (NF-κB) was examined using electrophoretic mobility shift assays. Luciferase reporter assay was used to detect the binding of NF-κB to the matrix metalloproteinase-9 (MMP-9) promoter. We found that T0901317 inhibited the invasion and migration of A549 cells in a dose-dependent manner. Meanwhile, we further indicated that activation of LXRβ, one subtype of LXRs, can downregulate MMP-9 expression. More importantly, activation of LXRβ triggered by T0901317 inhibited the invasion and metastasis of A549 cells by repressing NF-κB/MMP-9 signaling pathway. Taken together, our study shows that activation of LXRs triggered by T0901317 inhibits the invasion and metastasis of human non-small-cell lung cancer by repressing the NF-κB/MMP-9 signaling pathway.

Engineering examples and finite element structural analysis software SATWE was used to calculate by adjusting the shear wall thickness and grade to study the dynamic characteristics of concrete structures; analysis of seismic shear, natural vibration cycles, maximum interlayer displacement angle, lateral displacement stiffness to weight ratio and shear weight ratio of these parameters change rules, resulting in changes in shear wall thickness greater impact on shear wall amount.

To examine the expression of carcinoembryonic antigen-related cell adhesion molecule 1(CEACAM1) and CD34 in gastric adenocarcinoma, and to investigate their relations with clinical pathology-related factors.Immunohistochemistry (IHC) was used to analyze the expression of CEACAM1 and CD34, and micro-vessel density (MVD) marked by CD34 was calculated in 208 cases of human gastric adenocarcinoma and in 56 cases of normal human gastric tissues.There was no expression of CEACAM1 in normal gastric mucosa. However, all of the gastric adenocarcinoma tissues expressed CEACAM1 with cytoplasmic and/or membranous staining. CEACAM1 expression was classified as high and low (137/208 vs. 71/208, 65.9% vs. 34.1%), the CD34-MVD value was significantly different between the two groups (P0.05). In addition, expressions of CEACAM1 and CD34 were significantly different from gastric adenocarcinoma to normal gastric tissues, respectively (P0.05). High CEACAM1 expression and high MVD value were positively associated with lymph nodes metastasis and TNM stage, but negatively related to pathological grade (P0.05). But they were irrelevant with tumor size, patients' age and gender (P0.05).The up-regulation of CEACAM1 expression may participate in tumorous angiogenesis, especially high expression of CEACAM1 promoted its capability of invasion and metastasis.

A simple transition-metal-free protocol for the synthesis of indoles has been developed using aryne cycloaddition. The in situ-generated arynes couple with α-amino ketones through a one-step N-arylation–nucleophilic addition process under mild conditions and efficiently produce multisubstituted N-arylindoles.

Green liquor, a process liquor of alkali recovery in pulp and papermaking industry, pretreatment used in poplar kraft pulping process and the influences of pretreatment conditions on pentosan extraction have been studied. The results show that the influence of green liquor dosage on pentosan extraction is the most remarkable, with temperature taking the second place and time the third. Under the conditions of liquor ratio 1:4, green liquor dosage 1.5 L/kg (based on oven dried material), temperature 100 °C, time 90 min, higher pentosan extraction ratio of 13.81% (based on the pentosan content of raw material) is achieved. The wood was pulped under normal KP conditions after pretreated by the optimal pentosan extraction conditions. Comparing two kinds of pulp with and without pretreatment at similar stock yield level, the results show that the former has a little lower viscosity, significantly decreased Kappa number and excellent paper physical properties.

Through analyzing the real collision process of a steel plate rolling sliding off a lorry and box girder flange of an overpass, the damage degree of box girder may be known, and in this article the collision process is also simulated by means of finite element method so that it may be obtained how the damaged girder collapses and the cracks propagate. Furthermore, the dynamic characteristics are studied during the collision process and the reliability of overpass section collision is analyzed from the angle of energy transfer and dissipation.

The multidrug resistance 1 (MDR1) gene product P-glycoprotein is an ATP-dependent efflux pump associated with chemotherapy failure in breast cancer. In the present study, we show that paclitaxel induces MDR1 expression in the MCF-7 breast cancer cell line in a MAPK/Egr-1-dependent manner. Paclitaxel exposure activated the Erk1/2/MAPK pathway and promoted the accumulation of the early response transcription factor Egr-1 in MCF-7 cells. Egr-1 binds to the GC element on the proximal MDR1 promoter to enhance MDR1 transcription. Loss of Egr-1 function in paclitaxel-resistant MCF-7 cells decreased MDR1 expression, whereas inhibiting Erk1/2 activity reduced both Egr-1 accumulation and MDR1 expression. These findings suggest that Erk1/2-induced Egr-1 accumulation activates MDR1 transcription and thereby induces the drug resistance observed in paclitaxel-resistant MCF-7 cells. Further mechanistic studies indicate that Egr-1 most likely does not induce the constitutive activation of Erk1/2 through its target gene geranylgeranyl diphosphate synthase (GGPPS), which regulates Ras prenylation. Indeed, our results suggest a novel pathway by which paclitaxel induces MDR1 expression, possibly illuminating a potential target pathway for the prevention of MDR1-mediated drug resistance.

By taking a simple supported concrete-filled steel truss bridge as the research object in Guangzhou, some jobs have been done such as the simulating calculation and the real-time monitoring of side span steel girder during the jacking construction procedure, and contrast analysis was conducted between the calculating stress value and the measured stress value, which may draw a conclusion that the safety could be satisfied during the jacking construction procedure

At present the concept of instantaneous equilibrium is often adopted for simulating the wellbore pressure and temperature distribution in heavy oil wells. In this paper, the mathematical model was built and the diffusion of gas in the oil phase was researched. It proves that the non-equilibrium phenomenon is not obvious in convenient oil wells and it could be overlooked, while in heavy oil wells the non-equilibrium phenomenon is too prominent to be overlooked. Basing on the mass conservation principle, the momentum conservation principle and the energy conservation principle, the calculated model for wellbore pressure and temperature in heavy oil wells considering of the non-equilibrium effect was established. One example shows that the model can accurately reflect the flowing laws in heavy oil wells, which has a relative value for engineering.

Bonding a strengthening plate to the tension face is an effective technique to repair reinforced concrete beams; however, much large shear stress along the adhesive line at the plate end will occur. The stress may cause the plate bonded at its end and the strengthened beams fail prematurely. In this paper, the finite element analysis software ANSYS was applied to study the interfacial stress distribution among the adhesive layer. The main factors affecting the peak values of the interfacial stress was also investigated entirely. The effect depends on two factors such as the distance of plate end from the support and plate thickness.

Emerging of nanotechnology represents development and achievements of modern materials science. Petroleum industry nowadays plays a leading role in development of energy industry. Therefore, the combination of the two is a valuable research subject. The prospect of application of nanometer material in injection-production, tertiary oil production, well sensing and imaging system and coating technology is presented herein. In addition, issues with respect to application of nanometer material in oil exploitation are discussed. Nanometer material and nanotechnology is the frontier technology in science and technology development in the past decades. It is predicated that today's ordinary original material be replaced by nanometer material and nanotechnology be a "key technology"[1] that will surpass internet and genetic technology.

He development effect of steam soak well is influenced by the combination of the following parameters: injection speed, dryness fraction of steam, cyclic steam injection volume, soaking time and bottom hole flowing pressure. Take the advantage of annealed genetic algorithm which characterize with the traits of fast search and globally optimization. Based on the mathematical model of the output of the steam soak well, and optimize injection production parameter of the steam soak well which is aimed at the cycle average daily profit maximum, and make the field application.

The development effect of steam flood well is influenced by the combination of the following parameters: injection speed, dryness fraction of steam, temperature of injection steam, and bottom hole flowing pressure. Taking the advantage of adaptive simulated annealing genetic algorithm with the characteristic of fast search and globally optimization, and combine with the mathematical model of the steam flood well. Maximization of the vapor-liquid interface factor is the target to optimize injection production parameter of the steam flood well, the results of the optimization shows that cumulative oil production increases obviously.

The supramolecular hydrogels derived from low-molecular-mass gelators represent a unique class of soft matters and have important potential applications in biomedical fields, separation technology and cosmetic science. However, they suffer usually from weak mechanical and viscoelastic properties. In this work, we carry out the in situ hybridization of clay nanoparticles (Laponite RD) into the supramolecular hydrogel formed from a low-molecular-mass hydrogelator, 2,6-di[N-(carboxyethyl carbonyl)amino]pyridine (DAP), and investigate the viscoelastic and structural characteristics of resultant hybrid hydrogel. It was found that a small concentration of Laponite RD could lead to a significant increase in the storage modulus, loss modulus or complex viscosity. Compared with neat DAP hydrogel, the hybrid hydrogel has a greater hydrogel strength and a lower relaxation exponent. In particular, the enhancement of the clay nanoparticles to the viscoelastic properties of the DAP hydrogel is more effective in the case of higher DAP concentration. By relating its macroscopic elastic properties to a scaling fractal model, such a hybrid hydrogel was confirmed to be in the strong-link regime and to have a more complex network structure with a higher fractal dimension when compared with neat DAP hydrogel.

Small molecular hydrogels hold important potential applications in bioengineering and biomedicine. However, they suffer usually from weak mechanical and water-retention properties. In this work, hydrophilic dextran polysaccharide was used for the first time to modify the properties of the supramolecular hydrogel derived from a low-molecular-mass gelator, 2, 6-di[N-(carboxyethyl carbonyl)amino]pyridine (DAP). When the DAP hydrogel was formed in aqueous dextran solution with a rather low viscosity, we found that it could be significantly reinforced with respect to its viscoelastic moduli, complex viscosity and hydrogel strength. Moreover, the modified DAP hydrogel has a higher bound water content. These property changes could be attributed to the formation of intermolecular hydrogen bonds between DAP and dextran. By relating the dynamic rheological data with a scaling model, it was found that native and modified DAP hydrogels had both a fractal aggregate structure and the incorporation of dextran resulted in an increase of the fractal dimension. Scanning electron microscopy revealed that a more complex network structure was formed in the DAP hydrogel with dextran.

Drought is one of the most adverse environmental factors that impact on plant growth and reduce crop yields. To decipher the molecular mechanism of drought tolerance, we constructed a cDNA library using suppression subtractive hybridization (SSH) and dissected the gene expression profiles in seedling and jointing wheat plants after stress with PEG-6000. A total of 2 046 ESTs from the jointing stage library (J-Lib) were allocated to 961 contigs. Among the ESTs, 265 uni-genes in 12 categories were identified on the basis of sequence similarities and functional classifications. Most were known to be involved in protection, directly or indirectly, against water stress. To determine differences in gene expression profiles for water stress responses at the jointing and seedling stages, data from the J-Lib were compared with those from a 2-leaf seedling library (S-Lib) constructed by the same method. Significant differences were observed between the two libraries for function-known genes; signal transduction genes were far more active at jointing than at the seedling stage.

CONTEXT: To systematically evaluate the effects of hepatoprotective agents, when delivered either alone or in combination with other antiviral or non-antiviral drugs in patients with hepatitis B and hepatic fibrosis. OBJECTIVES: The current randomized controlled clinical trials aimed to evaluate the efficacy of combinations of antiviral and non-antiviral hepatoprotective agents on indexes of liver function and liver fibrosis in patients with hepatitis B. DATA SOURCES: Published literatures in Chinese and English on hepatoprotective treatment strategies for chronic hepatitis B and liver fibrosis were searched in three databases and randomized controlled clinical trials were selected. STUDY SELECTION: Data were extracted according to a variety of inclusion and exclusion criteria. Meta-analysis was employed to analyze the data. RESULTS: A total of 22 randomized controlled trials encompassing 1,714 cases were considered in the meta-analysis. The obtained results indicated that the combination of antiviral drug and hepatoprotective agent was better than antiviral drug alone to improve liver function. Similarly, regarding liver fibrosis, using two different hepatoprotective agents was better than using one agent. The normalization rates of Aminotransferase (ALT) and total Bilirubin (TBil) were improved 25.7% by two hepatoprotective agents compared to the single agent. Acetylcysteine was superior to ursodeoxycholic acid or silibinin to reduce ALT. Ursodeoxycholic acid was superior to acetylcysteine or silibinin to reduce TBIL. CONCLUSIONS: Hepatoprotective agents combined with antiviral drugs can significantly improve liver function and liver fibrosis parameters in patients with hepatitis B.

A simple transition-metal-free protocol for the synthesis of indoles has been developed using aryne cycloaddition. The in situ-generated arynes couple with α-amino ketones through a one-step N-arylation–nucleophilic addition process under mild conditions and efficiently produce multisubstituted N-arylindoles.

Based on a large amount of investigation, analysis and research of the application system status and network status of the power grid, the paper introduces a novel CDN network architecture for power grid, which has the power grid characteristics and meets the power grid requirements. In order to ensure the efficiency and stability of CDN system, the CDN network architecture key technologies include virtual CDN technology, software defined CDN technology, network measurement technology based on data analysis, database for CDN network, CDN management platform and load balance. Also, there are four aspects to ensure the security of CDN system, as network security, safety management system, CDN equipment safety and anti-leech mechanism which can be a reference for the construction of CDN network in power grid and other industries.

Guidan Highway super large bridge of Guangzhu railway engineering is a three-span (60m+100m+60m) continuous box girder bridge, which was constructed by using hanging basket cantilever method. On the basis of the analytic theory of 3D finite element forward/reverse, the linear monitoring of the whole bridge was done through controlling the template elevation of box girder (pre-camber). The result of monitoring shows that the linear of construction satisfies the requirements of design and specification and the bridge can be closed smoothly.

In situ crosslinkable hydrogel formed from an amphiphilic amylopectin-based hydrogelator in aqueous solution was investigated with respect to its viscoelasticity, structure as well as protein encapsulation and release. Different from the physical hydrogel formed from an aqueous amylopectin system of sufficiently high concentration, such a hydrogel could be formed rapidly at room temperature and exhibit enhanced viscoelastic properties, mechanical strength, and shear thinning behavior. In addition, it has a more complex network structure with a higher fractal dimension due to intermolecular hydrophobic interactions and macromolecular chain entanglements. By circular dichroism analyses and in vitro release experiments, this hydrogel material was found to have a great potential as new matrix for the entrapment and sustained release of bovine serum albumin.

The magnetic properties of Tb(Fe 1− x Co x ) 11.3 Nb 0.7 compounds with x =0, 0.05, 0.1, 0.15, 0.2 and 0.3 have been investigated. All compounds studied crystallize in the ThMn 12 -type of structure. Substitution of Co for Fe leads to a contraction of the unit-cell volume. The Curie temperature clearly increases with increasing Co content from 551 K for x =0 to 831 K for x =0.3. The magnetic moment of the transition-metal sublattice increases with increasing Co content from 22.2 μ B /f.u. for x =0 to 23.1 μ B /f.u. for x =0.3. As the temperature increases, a spin reorientation from easy-plane to easy-cone is found in all compounds investigated. The spin-reorientation temperatures T sr have been derived from the temperature dependence of the magnetization in a low field and decrease monotonously with increasing Co content. The easy magnetization direction at room temperature has been determined by X-ray diffraction on magnetically-aligned powder samples. The influence of the substitution of Co for Fe on the magnetic anisotropy is discussed in terms of crystal-field theory.