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ERDC/EL TR-18-12 Aquatic Plant Control Research Program (APCRP) Abiotic Removal of Phosphate From Surface Water – Bench Scale to Meso-Scale Environmental Laboratory Carina M. Jung, Jed O. Eberly, G. Alon Blakeney, and Heather M. Knotek-Smith October 2018 Approved for public release; distribution is unlimited. The U.S. Army Engineer Research and Development Center (ERDC) solves the nation’s toughest engineering and environmental challenges. ERDC develops innovative solutions in civil and military engineering, geospatial sciences, water resources, and environmental sciences for the Army, the Department of Defense, civilian agencies, and our nation’s public good. Find out more at www.erdc.usace.army.mil. To search for other technical reports published by ERDC, visit the ERDC online library at http://acwc.sdp.sirsi.net/client/default. Aquatic Plant Control Research Program (APCRP) ERDC/EL TR-18-12 October 2018 Abiotic Removal of Phosphate From Surface Water – Bench Scale to Meso-Scale Carina M. Jung, Jed O. Eberly, G. Alon Blakeney, and Heather M. Knotek-Smith Environmental Laboratory US Army Engineer Research and Development Center 3909 Halls Ferry Road Vicksburg, MS 39180-6199 Final Report Approved for public release; distribution is unlimited. Prepared for Headquarters, U.S. Army Corps of Engineers Washington, DC 20314-1000 Under Project Number 454747 ERDC/EL TR-18-12 ii Abstract Cultural eutrophication is a primary cause of impairment to recreational and commercially important waterways. Nutrient loading of waterways through release of fertilizers and sewage, among other substrates high in growth-limiting substrates (phosphorus, nitrogen, and carbon) allows for unchecked growth of photosynthesizers such as blue-green algae (cyanobacteria). Significant cyanobacterial growth may result in plant die-off, hypoxia, or anoxia, and the proliferation of harmful algal blooms (HABs). Methods exist to bind and inactivate nutrients like phosphorous but these do not remove nutri

ERDC/EL TR-18-8 Aquatic Plant Control Research Program (APCRP) Biological Phosphorous Removal From Impacted Water Environmental Laboratory Carina M. Jung and Jed O. Eberly August 2018 Approved for public release; distribution is unlimited. The U.S. Army Engineer Research and Development Center (ERDC) solves the nation’s toughest engineering and environmental challenges. ERDC develops innovative solutions in civil and military engineering, geospatial sciences, water resources, and environmental sciences for the Army, the Department of Defense, civilian agencies, and our nation’s public good. Find out more at www.erdc.usace.army.mil. To search for other technical reports published by ERDC, visit the ERDC online library at http://acwc.sdp.sirsi.net/client/default. Aquatic Plant Control Research Program (APCRP) ERDC/EL TR-18-8 August 2018 Biological Phosphorous Removal From Impacted Water Carina M. Jung and Jed O. Eberly U.S. Army Engineer Research and Development Center (ERDC) Environmental Laboratory 3909 Halls Ferry Road Vicksburg, MS 39180-6199 Final Report Approved for public release; distribution is unlimited. Prepared for Headquarters, U.S. Army Corps of Engineers Washington, DC 20314-1000 Under “Biological Phosphorous Removal from Impacted Water, Project Number 454747.” ERDC/EL TR-18-8 ii Abstract Nutrient input into waterways from agriculture runoff, sewage, and other sources is a major cause of water pollution and cultural eutrophication. Nitrogen and phosphate are growth-limiting nutrients, that when present in high concentrations, allow for unchecked growth of cyanobacteria, resulting in reduced water quality and the potential for proliferation of harmful algal blooms (HABs). Physical removal of these nutrients from waterways is needed. Immobilized phosphorous accumulating bacteria could be used as a phosphate biofilter to absorb and accumulate phosphate from impacted water. The research presented herein demonstrates the immobilization or encapsulation of ph

ERDC TR-18-10 U.S. Army BT25 Basic Research Program Flavonoids Differentially Influence Rhizosphere Bacterial Communities from Native and Introduced Lespedeza Roots Engineer Research and Development Center Ryan R. Busby, David B. Ringelberg, and Carina M. Jung August 2018 Approved for public release; distribution is unlimited. The U.S. Army Engineer Research and Development Center (ERDC) solves the nation’s toughest engineering and environmental challenges. ERDC develops innovative solutions in civil and military engineering, geospatial sciences, water resources, and environmental sciences for the Army, the Department of Defense, civilian agencies, and our nation’s public good. Find out more at www.erdc.usace.army.mil. To search for other technical reports published by ERDC, visit the ERDC online library at http://acwc.sdp.sirsi.net/client/default. Cover Photo: Closeup of Lespedeza cuneata; online image source: Invasive Species and Ecosystem Health (https://www.invasive.org/browse/subinfo.cfm?sub=3033). U.S. Army BT25 Basic Research Program ERDC TR-18-10 August 2018 Flavonoids Differentially Influence Rhizosphere Bacterial Communities from Native and Introduced Lespedeza Roots Ryan R. Busby Construction Engineering Research Laboratory U.S. Army Engineer Research and Development Center 2902 Newmark Drive Champaign, IL 61822 David B. Ringelberg Cold Regions Research and Engineering Laboratory U.S. Army Engineer Research and Development Center 72 Lyme Road Hanover, NH 03755 Carina M. Jung Environmental Laboratory U.S. Army Engineer Research and Development Center 3909 Halls Ferry Road Vicksburg, MS 39180 Final report Approved for public release; distribution is unlimited. Prepared for Assistant Secretary of the Army (Acquisition, Logistics and Technology) 103 Army Pentagon Washington, DC 20314-1000 Under Project 14-05, “Molecular Impact of Invasive Species Introduction on Military Lands” ERDC TR-18-10 ii Abstract Military training can create disturbances that facilitat

ERDC/EL TR-18-5 Evaluation of Sulfide Emissions from a Hydraulic System at the Blue River Dam Environmental Laboratory Victor F. Medina, Michelle Wynter, Carina Jung, Amber Russell, Timothy Paulus, and Chris S. Griggs April 2018 Approved for public release; distribution is unlimited. The U.S. Army Engineer Research and Development Center (ERDC) solves the nation’s toughest engineering and environmental challenges. ERDC develops innovative solutions in civil and military engineering, geospatial sciences, water resources, and environmental sciences for the Army, the Department of Defense, civilian agencies, and our nation’s public good. Find out more at www.erdc.usace.army.mil. To search for other technical reports published by ERDC, visit the ERDC online library at http://acwc.sdp.sirsi.net/client/default. ERDC/EL TR-18-5 April 2018 Evaluation of Sulfide Emissions from a Hydraulic System at the Blue River Dam Victor F. Medina, Michelle Wynter, Carina Jung, Amber Russell, and Chris S. Griggs. Environmental Laboratory U.S. Army Engineer Research and Development Center 3909 Halls Ferry Road Vicksburg, MS 39180-6199 Timothy Paulus St. Paul District (MVP) U.S. Army Corps of Engineers 180 5th Street East, Suite 700 St. Paul, MN 55101 Final report Approved for public release; distribution is unlimited. Prepared for U.S. Army Corps of Engineers Washington, DC 20314-1000 Under Work Unit 33143 ERDC/EL TR-18-5 ii Abstract Hydrogen sulfide releases occurred during a routine maintenance process in a hydraulic oil system at Blue River Dam, Oregon. The project worked under the hypothesis that the sulfide emissions most likely resulted from reductive biological processes. Hydraulic oil samples were collected from the Blue River Dam, and from two other nearby dams with similar hydraulic systems, Hills Creek Dam, and Cougar Dam. Water samples from the reservoir were also collected. Sulfur was found in all the oil and water samples, however, no patterns with sulfur to other parameters

ERDC/EL TR-17-11 Review and Evaluation of Reservoir Management Strategies for Harmful Algal Blooms Environmental Laboratory Brook D. Herman, Jed O. Eberly, Carina M. Jung, and Victor F. Medina July 2017 Approved for public release; distribution is unlimited. The U.S. Army Engineer Research and Development Center (ERDC) solves the nation’s toughest engineering and environmental challenges. ERDC develops innovative solutions in civil and military engineering, geospatial sciences, water resources, and environmental sciences for the Army, the Department of Defense, civilian agencies, and our nation’s public good. Find out more at www.erdc.usace.army.mil. To search for other technical reports published by ERDC, visit the ERDC online library at http://acwc.sdp.sirsi.net/client/default. Water Operations Technical Support Program (WOTS) ERDC/EL TR-17-11 July 2017 Review and Evaluation of Reservoir Management Strategies for Harmful Algal Blooms Brook D. Herman, Jed O. Eberly, Carina M. Jung, and Victor F. Medina Environmental Laboratory U.S. Army Engineer Research and Development Center 3909 Halls Ferry Road Vicksburg, MS 39180 Final report Approved for public release; distribution is unlimited. Prepared for U.S. Army Corps of Engineers Washington, DC 20314-1000 Under Project Number WOTS-16-014, “Review and Evaluation of Reservoir Management Strategies for Harmful Algal Blooms” ERDC/EL TR-17-11 ii Abstract The purpose of this report is to review and evaluate available infor-mation regarding reservoir operation strategies for management of harmful algal blooms (HABs). HABs can be problematic, creating eu-trophication, and taste and odor issues. HABs also involve the release of toxins in the water column, which can cause sickness by ingestion and skin contact. This report presents the results of a review of journal articles, reports, published accounts of potential management options, effectiveness of management, and potential impacts of management actions on lake/reservoir ec

Objectives of this project were to: (a) elucidate RDX degradation pathways in model RDX-degrading bacteria, (b) design and develop molecular tools to identify genes responsible for RDX biodegradation, and (c) correlate the response of biomarker(s) to concentrations of RDX and/or rates of RDX degradation. Gordonia sp. KTR9 and Shewanella oneidensis MR-1served as model bacterial systems for the aerobic and anaerobic degradation of RDX, respectively. Genome annotation and functional characterization of the plasmid pGKT2 in KTR9 revealed that xplA gene is both necessary and sufficient for RDX degradation. Shewanella oneidensis MR-1 was shown to efficiently degrade RDX anaerobically via two initial routes: (a) sequential N-NO2 reduction to the corresponding nitroso (N-NO) derivatives; and (b) mono-denitration followed by ring cleavage. The qPCR molecular tools described in this report have the potential to be used by remediation specialists for site characterization, treatment recommendations, and for evaluation and optimization of the treatment process. The fundamental information gained in this study suggests that XplA-mediated aerobic denitration of RDX may be subjected to inhibitory effects in response to nitrogen availability. Additional research is required to determine reliable guidelines to inform site managers of specific field concentrations of ammonium and nitrate that will increase RDX treatment times. Also, techniques to effectively lower the inorganic nitrogen concentrations to non-inhibitory levels for the aerobic RDX biodegradation pathway will need to be determined., Prepared in cooperation with the Department of Microbiology and Immunology, University of British Columbia, Vancouver, and the Biotechnology Research Institute, NRC, Montreal, Quebec, Canada.

ERDC/EL TR-12-33 Strategic Environmental Research and Development Program Identification of Microbial Gene Biomarkers for in situ RDX Biodegradation Project ER-1609 Environmental Laboratory Fiona. H. Crocker, Karl J. Indest, Carina M. Jung, Dawn E. Hancock, Megan E. Merritt, Christine Florizone, Hao-Ping Chen, Gordon R. Stewart, Songhua Zhu, Nicole Sukdeo, Marie-Claude Fortin, Steven J. Hallam, William W. Mohn, Lindsay D. Eltis, Nancy N. Perreault, Jian-Shen Zhao, Louise Paquet, Annamaria Halasz, and Jalal Hawari December 2012 Approved for public release; distribution is unlimited. Strategic Environmental Research and Development Program ERDC/EL TR-12-33 December 2012 Identification of Microbial Gene Biomarkers for in situ RDX Biodegradation Project ER-1609 Fiona. H. Crocker, Karl J. Indest, Carina M. Jung, Dawn E. Hancock, and Megan E. Merritt Environmental Laboratory U.S. Army Engineer Research and Development Center 3909 Halls Ferry Road Vicksburg, MS 39180-6199 Christine Florizone, Hao-Ping Chen, Gordon R. Stewart, Songhua Zhu, Nicole Sukdeo, Marie-Claude Fortin, Steven J. Hallam, William W. Mohn, and Lindsay D. Eltis Department of Microbiology and Immunology University of British Columbia 2350 Health Sciences Mall Vancouver, British Columbia Canada V6T 1Z3 Nancy N. Perreault, Jian-Shen Zhao, Louise Paquet, Annamaria Halasz and Jalal Hawari Biotechnology Research Institute, NRC 6100 Royalmount Avenue Montreal, Quebec Canada H4P 2R2 Final report Approved for public release; distribution is unlimited. Prepared for U.S. Army Corps of Engineers Washington, DC 20314-1000 ERDC/EL TR-12-33 ii Abstract Objectives of this project were to: (a) elucidate RDX degradation pathways in model RDX-degrading bacteria, (b) design and develop molecular tools to identify genes responsible for RDX biodegradation, and (c) correlate the response of biomarker(s) to concentrations of RDX and/or rates of RDX degradation. Gordonia sp. KTR9 and Shewanella oneidensis MR-1 served as model bac