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Radon inhalation decreases the level of lipid peroxide (LPO); this is attributed to the activation of antioxidative functions. This activation contributes to the beneficial effects of radon therapy, but there are no studies on the risks of radon therapy, such as DNA damage. We evaluated the effect of radon inhalation on DNA damage caused by oxidative stress and explored the underlying mechanisms. Mice were exposed to radon inhalation at concentrations of 2 or 20 kBq/m3 (for one, three, or 10 days). The 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels decreased in the brains of mice that inhaled 20 kBq/m3 radon for three days and in the kidneys of mice that inhaled 2 or 20 kBq/m3 radon for one, three or 10 days. The 8-OHdG levels in the small intestine decreased by approximately 20–40% (2 kBq/m3 for three days or 20 kBq/m3 for one, three or 10 days), but there were no significant differences in the 8-OHdG levels between mice that inhaled a sham treatment and those that inhaled radon. There was no significant change in the levels of 8-oxoguanine DNA glycosylase, which plays an important role in DNA repair. However, the level of Mn-superoxide dismutase (SOD) increased by 15–60% and 15–45% in the small intestine and kidney, respectively, following radon inhalation. These results suggest that Mn-SOD probably plays an important role in the inhibition of oxidative DNA damage.

Radon inhalation activates antioxidative functions in mouse organs, thereby contributing to inhibition of oxidative stress-induced damage. However, the specific redox state of each organ after radon inhalation has not been reported. Therefore, in this study, we evaluated the redox state of various organs in mice following radon inhalation at concentrations of 2 or 20 kBq/m3 for 1, 3 or 10 days. Scatter plots were used to evaluate the relationship between antioxidative function and oxidative stress by principal component analysis (PCA) of data from control mice subjected to sham inhalation. The results of principal component (PC) 1 showed that the liver and kidney had high antioxidant capacity; the results of PC2 showed that the brain, pancreas and stomach had low antioxidant capacities and low lipid peroxide (LPO) content, whereas the lungs, heart, small intestine and large intestine had high LPO content but low antioxidant capacities. Furthermore, using the PCA of each obtained cluster, we observed altered correlation coefficients related to glutathione, hydrogen peroxide and LPO for all groups following radon inhalation. Correlation coefficients related to superoxide dismutase in organs with a low antioxidant capacity were also changed. These findings suggested that radon inhalation could alter the redox state in organs; however, its characteristics were dependent on the total antioxidant capacity of the organs as well as the radon concentration and inhalation time. The insights obtained from this study could be useful for developing therapeutic strategies targeting individual organs.

The forced swim test (FST) is a screening model for antidepressant activity; it causes immobility and induces oxidative stress. We previously reported that radon inhalation has antidepressant-like effects in mice potentially through the activation of antioxidative functions upon radon inhalation. This study aimed to investigate the effect of prior and post low-dose X-irradiation (0.1, 0.5, 1.0 and 2.0 Gy) on FST-induced immobility and oxidative stress in the mouse brain, and the differences, if any, between the two. Mice received X-irradiation before or after the FST repeatedly for 5 days. In the post-FST-irradiated group, an additional FST was conducted 4 h after the last irradiation. Consequently, animals receiving prior X-irradiation (0.1 Gy) had better mobility outcomes than sham-irradiated mice; however, their levels of lipid peroxide (LPO), an oxidative stress marker, remained unchanged. However, animals that received post-FST X-irradiation (0.5 Gy) had better mobility outcomes and their LPO levels were significantly lower than those of the sham-irradiated mice. The present results indicate that 0.5 Gy X-irradiation after FST inhibits FST-induced immobility and oxidative stress in mice.

A diblock copolymer (P98N100) composed of a biocompatible water-soluble block (PMPC) and a lower critical solution temperature (LCST) type thermo-responsive block (PNIPAM) was prepared via controlled radical polymerization. To dissolve fullerene (C60) in water, the C60/P98N100 complex was prepared by mixing C60 and P98N100 powders. The maximum solubilized C60 concentration in water was 1.39 g/L, as estimated from UV–vis adsorption, when the polymer concentration was 5.0 g/L. The percent transmittance of the aqueous solution of the C60/P98N100 complex decreased above 36 °C due to inter-complex association above the LCST for the PNIPAM block. While the hydrodynamic radius of C60/P98N100 complex was 135 nm at 20 °C, it increased to 161 nm at 50 °C. Despite the observation of 1H NMR signals from PMPC and PNIPAM blocks for the C60/P98N100 complex in D2O at room temperature, the signals from PNIPAM disappeared above 35 °C due to restricted motion of PNIPAM. Generation of singlet oxygen (1O2) from the C60/P98N100 complex by photo-irradiation was confirmed using 9,10-anthracene dipropionic acid (ADPA). The absorbance of ADPA decreased with increasing irradiation time due to oxidation of ADPA by 1O2. It is expected that the C60/P98N100 complex can be applied as a thermo-responsive carrier for photodynamic therapy. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017

[1] The continuous measurement of river discharge for long periods of time is crucial in water resource studies. However, the accurate estimation of river discharge is a difficult and labor-intensive procedure; thus, a robust and efficient method of measurement is required. Continuous measurements of flowrate have been carried out in a wide, shallow gravel bed river (water depth ≈ 0.6 m under low-flow conditions, width ≈ 115 m) using Fluvial Acoustic Tomography System (FATS) that has 25 kHz broadband transducers with horizontally omnidirectional and vertically hemispherical beam patterns. Reciprocal sound transmissions were performed between the two acoustic stations located diagonally on both sides of the river. The horizontal distance between the transducers was 301.96 m. FATS enabled the measurement of the depth- and range-averaged sound speed and flow velocity along the ray path. In contrast to traditional point/transect measurements of discharge, in a fraction of a second, FATS covers the entire cross section of river in a single measurement. The flow rates measured by FATS were compared to those estimated by moving boat Acoustic Doppler Current Profiler (ADCP) and rating curve (RC) methods. FATS estimates were in good agreement with ADCP estimates over a range of 20 to 65 m3 s−1. The RMS of residual between the two measurements was 2.41 m3 s−1. On the other hand the flowrate by RC method fairly agreed with FATS estimates for greater discharges than around 40 m3 s−1. This inconsistency arises from biased RC estimates in low flows. Thus, the flow rates derived from FATS could be considered reliable.

In this study the sub-tidal and intra-tidal variations of salt fluxes in the upstream section of a shallow estuary (with a water depth of less than 3 m) were investigated. The salt fluxes were estimated based on the cross-sectional average salinity and velocity measured by the fluvial acoustic tomography system (FATS). The results indicate that the magnitude of seaward fluxes is approximately two times greater than that of landward fluxes under normal conditions. The results of short-term observation in the study area indicate that there is a phase lag of the bottom and surface salinities between the regions with the largest and smallest depths. The vertical shear flux with a peak value of −0.7 m2/s during the ebb tide indicated an important contribution to the total salt flux compared with the advective flux. A phase lag occurred between the vertical shear terms in the regions with the largest and smallest depths, which resulted from the correlation between the vertical variations of the salinity and velocity and the existence of transversal velocity circulations.