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Made available in DSpace on 2019-09-12T16:53:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2009 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The study objective was to evaluate the effects of photodynamic therapy on buccal candidiasis in rats. After experimental candidiasis had been induced on the tongue dorsum, 72 rats were distributed into four groups according to treatment: treated with laser and methylene blue photosensitizer (L+P+); treated only with laser (L+P-); treated only with photosensitizer (L--P+); not treated with laser or photosensitizer (L-P-). The rats were killed immediately, 1 day, or 5 days after treatment, for microscopic analysis of the tongue dorsum. Observation verified that the photodynamic therapy group (L+P+) exhibited fewer epithelial alterations and a lower chronic inflammatory response than the L-P- group. The group L+P- presented more intense epithelial alterations and chronic inflammatory response than the remaining groups. The L-P+ group showed tissue lesions similar to those of the L-P- group. In conclusion, rats treated with photodynamic therapy developed more discrete candidiasis lesions than did the remaining groups. [Junqueira, Juliana Campos; Martins, Joyce da Silva; Faria, Raquel Lourdes; Dias Colombo, Carlos Eduardo; Cardoso Jorge, Antonio Olavo] Sao Paulo State Univ, Sch Dent Sao Jose Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Paulo, Brazil [Dias Colombo, Carlos Eduardo] Universidade de Taubaté (Unitau), Dept Dent, Sao Paulo, Brazil

Made available in DSpace on 2019-09-12T16:53:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2005 O objetivo deste estudo foi analisar o desenvolvimento de candidose e a recuperação de C. albicans da cavidade oral de ratos ovariectomizados e sham ‐ ovariectomizados. Cento e vinte e quatro ratos originalmente negativos para Candida spp. na cavidade oral foram divididos em dois grupos: ovariectomizados e sham ‐ ovariectomizados. Cinqüenta e oito ratos ovariectomizados e a mesma quantidade de ratos sham ‐ ovariectomizados foram inoculados com C. albicanspara o estudo do desenvolvimento da candidose e recuperação de leveduras. Quatro animais de cada grupo não foram inoculados com suspensão de leveduras e submetidos à análise morfológica do dorso da língua por microscopia óptica e eletrônica de varredura. O desenvolvimento de candidose no dorso da língua foi observado por microscopia óptica e eletrônica de varredura nos períodos de 6 horas, 24 horas, 7 dias e 15 dias após a última inoculação. A recuperação de C. albicans foi realizada por amostras orais em placas de ágar Sabouraud após 1, 2, 5 e 7 dias e progressivamente a cada intervalo de 15 dias, até a obtenção de culturas negativas para leveduras. Os resultados foram analisados ​​pelos testes t de Mann ‐ Whitney e Student . Dorso da língua de ratos sham ‐ ovariectomizados e ovariectomizados, não infectados por Candida, apresentou aspecto normal. Entre os ratos infectados, o grupo ovariectomizado apresentou menor ocorrência de lesões de candidose e menor recuperação de C. albicans da cavidade oral em relação ao grupo simulado ovariectomizado. Pode-se concluir que a candidose foi menos frequente nas cavidades orais de ratos ovariectomizados em relação aos simulados ovariectomizados. The aim of this study was to analyze the development of candidosis and the recovery of C albicans from the oral cavity of ovariectomized and sham-ovariectomized rats. One hundred and twenty-four rats originally negative for Candida spp. in the oral cavity were divided into two groups: ovariectomized and sham-ovariectomized. Fifty-eight ovariectomized and the same quantity of sham-ovariectomized rats were inoculated with C albicans for the study of candidosis development and recovery of yeast. Four animals from each group were not inoculated with yeast suspension and were submitted to tongue dorsum morphologic analysis by optical and scanning electron microscopy. The development of candidosis in the tongue dorsum was observed by optical and scanning electron microscopy in the periods of 6 hr, 24 hr, 7 days and 15 days after the last inoculation. Recovery of C albicans was performed by oral samples plating on Sabouraud agar after 1, 2, 5 and 7 days and progressively at each 15-day interval until negative cultures for yeasts were obtained. The results were analyzed by Mann-Whitney and Student's t tests. The tongue dorsum of sham-ovariectomized and ovariectomized rats, not infected by Candida, presented normal aspect. Among the infected rats, the ovariectomized group showed less occurrence of candidosis lesions and lower recovery of C albicans from the oral cavity in relation to the sham-ovariectomized group. It could be concluded that candidosis was less frequent from the oral cavities of ovariectomized rats in relation to sham-ovariectomized. Univ Sao Paulo, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Paulo, Brazil; Universidade de Taubaté (Unitau), Dept Dent, BR-12020270 Taubate, SP, Brazil