Search

Your search keyword '"Just, Annette"' showing total 33 results
Start Over Author "Just, Annette"
33 results on '"Just, Annette"'

2. Ex vivo modelling of cardiac injury identifies ferroptosis-related pathways as a potential therapeutic avenue for translational medicine

Author

Abbas, Naisam, Bentele, Marco, Waleczek, Florian J.G., Fuchs, Maximilian, Just, Annette, Pfanne, Angelika, Pich, Andreas, Linke, Sophie, Neumüller, Susanne, Stucki-Koch, Angelika, Jordan, Maria, Perbellini, Filippo, Werlein, Christopher, Korte, Wilhelm, Ius, Fabio, Ruhparwar, Arjang, Weber, Natalie, Fiedler, Jan, and Thum, Thomas

Published
2024
Full Text
View/download PDF

4. Inhibition of miR-21: cardioprotective effects in human failing myocardium ex vivo

Author

Abbas, Naisam, primary, Haas, Jonas A, additional, Xiao, Ke, additional, Fuchs, Maximilian, additional, Just, Annette, additional, Pich, Andreas, additional, Perbellini, Filippo, additional, Werlein, Christopher, additional, Ius, Fabio, additional, Ruhparwar, Arjang, additional, Fiedler, Jan, additional, Weber, Natalie, additional, and Thum, Thomas, additional

Published
2024
Full Text
View/download PDF

5. Combined high-throughput library screening and next generation RNA sequencing uncover microRNAs controlling human cardiac fibroblast biology

Author

Schimmel, Katharina, Stojanović, Stevan D., Huang, Cheng-Kai, Jung, Mira, Meyer, Martin H., Xiao, Ke, Grote-Levi, Lea, Bär, Christian, Pfanne, Angelika, Mitzka, Saskia, Just, Annette, Geffers, Robert, Bock, Katharina, Kenneweg, Franziska, Kleemiß, Felix, Falk, Christine S., Fiedler, Jan, and Thum, Thomas

Published
2021
Full Text
View/download PDF

6. Pleiotropic cardiac functions controlled by ischemia-induced lncRNA H19

Author

Hobuß, Lisa, Foinquinos, Ariana, Jung, Mira, Kenneweg, Franziska, Xiao, Ke, Wang, Yong, Zimmer, Karina, Remke, Janet, Just, Annette, Nowak, Juliette, Schmidt, Arne, Pich, Andreas, Mazlan, Stephane, Reamon-Buettner, Stella M., Ramos, Gustavo Campos, Frantz, Stefan, Viereck, Janika, Loyer, Xavier, Boulanger, Chantal, Wollert, Kai C., Fiedler, Jan, and Thum, Thomas

Published
2020
Full Text
View/download PDF

10. Long Noncoding RNA-Enriched Vesicles Secreted by Hypoxic Cardiomyocytes Drive Cardiac Fibrosis

Author

Kenneweg, Franziska, Bang, Claudia, Xiao, Ke, Boulanger, Chantal M., Loyer, Xavier, Mazlan, Stephane, Schroen, Blanche, Hermans-Beijnsberger, Steffie, Foinquinos, Ariana, Hirt, Marc N., Eschenhagen, Thomas, Funcke, Sandra, Stojanovic, Stevan, Genschel, Celina, Schimmel, Katharina, Just, Annette, Pfanne, Angelika, Scherf, Kristian, Dehmel, Susann, Raemon-Buettner, Stella M., Fiedler, Jan, and Thum, Thomas

Published
2019
Full Text
View/download PDF

12. Natural Compound Library Screening Identifies New Molecules for the Treatment of Cardiac Fibrosis and Diastolic Dysfunction

Author

Schimmel, Katharina, Jung, Mira, Foinquinos, Ariana, José, Gorka San, Beaumont, Javier, Bock, Katharina, Grote-Levi, Lea, Xiao, Ke, Bär, Christian, Pfanne, Angelika, Just, Annette, Zimmer, Karina, Ngoy, Soeun, López, Begoña, Ravassa, Susana, Samolovac, Sabine, Janssen-Peters, Heike, Remke, Janet, Scherf, Kristian, Dangwal, Seema, Piccoli, Maria-Teresa, Kleemiss, Felix, Kreutzer, Fabian Philipp, Kenneweg, Franziska, Leonardy, Julia, Hobuß, Lisa, Santer, Laura, Do, Quoc-Tuan, Geffers, Robert, Braesen, Jan Hinrich, Schmitz, Jessica, Brandenberger, Christina, Müller, Dominik N., Wilck, Nicola, Kaever, Volkhard, Bähre, Heike, Batkai, Sandor, Fiedler, Jan, Alexander, Kevin M., Wertheim, Bradley M., Fisch, Sudeshna, Liao, Ronglih, Diez, Javier, González, Arantxa, and Thum, Thomas

Published
2020
Full Text
View/download PDF

14. Deciphering Pro-angiogenic Transcription Factor Profiles in Hypoxic Human Endothelial Cells by Combined Bioinformatics and in vitro Modeling

Author

Schmidt, Arne, primary, Fuchs, Maximilian, additional, Stojanović, Stevan D., additional, Liang, Chunguang, additional, Schmidt, Kevin, additional, Jung, Mira, additional, Xiao, Ke, additional, Weusthoff, Jan, additional, Just, Annette, additional, Pfanne, Angelika, additional, Distler, Jörg H. W., additional, Dandekar, Thomas, additional, Fiedler, Jan, additional, Thum, Thomas, additional, and Kunz, Meik, additional

Published
2022
Full Text
View/download PDF

15. Deciphering Pro-angiogenic Transcription Factor Profiles in Hypoxic Human Endothelial Cells by Combined Bioinformatics and in vitro Modeling

Author

Schmidt, Arne, Fuchs, Maximilian, Stojanović, Stevan D., Liang, Chunguang, Schmidt, Kevin, Jung, Mira, Xiao, Ke, Weusthoff, Jan, Just, Annette, Pfanne, Angelika, Distler, Jörg H. W., Dandekar, Thomas, Fiedler, Jan, Thum, Thomas, Kunz, Meik, and Publica

Subjects
ddc:610, Cardiology and Cardiovascular Medicine
Abstract

BackgroundConstant supply of oxygen is crucial for multicellular tissue homeostasis and energy metabolism in cardiac tissue. As a first response to acute hypoxia, endothelial cells (ECs) promote recruitment and adherence of immune cells to the dysbalanced EC barrier by releasing inflammatory mediators and growth factors, whereas chronic hypoxia leads to the activation of a transcription factor (TF) battery, that potently induces expression of growth factors and cytokines including platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). We report a hypoxia-minded, targeted bioinformatics approach aiming to identify and validate TFs that regulate angiogenic signaling.ResultsA comprehensive RNA-Seq dataset derived from human ECs subjected to normoxic or hypoxic conditions was selected to identify significantly regulated genes based on (i) fold change (normoxia vs. hypoxia) and (ii) relative abundancy. Transcriptional regulation of this gene set was confirmed via qPCR in validation experiments where HUVECs were subjected to hypoxic conditions for 24 h. Screening the promoter and upstream regulatory elements of these genes identified two TFs, KLF5 and SP1, both with a potential binding site within these regions of selected target genes. In vitro, siRNA experiments confirmed SP1- and KLF5-mediated regulation of identified hypoxia-sensitive endothelial genes. Next to angiogenic signaling, we also validated the impact of TFs on inflammatory signaling, both key events in hypoxic sensing. Both TFs impacted on inflammatory signaling since endogenous repression led to increased NF-κB signaling. Additionally, SP1 silencing eventuated decreased angiogenic properties in terms of proliferation and tube formation.ConclusionBy detailed in silico analysis of promoter region and upstream regulatory elements for a list of hypoxia-sensitive genes, our bioinformatics approach identified putative binding sites for TFs of SP or KLF family in vitro. This strategy helped to identify TFs functionally involved in human angiogenic signaling and therefore serves as a base for identifying novel RNA-based drug entities in a therapeutic setting of vascularization.

Published
2022

16. Cardiac fibroblast-derived microRNA passenger strand-enriched exosomes mediate cardiomyocyte hypertrophy

Author

Bang, Claudia, Batkai, Sandor, Dangwal, Seema, Gupta, Shashi Kumar, Foinquinos, Ariana, Holzmann, Angelika, Just, Annette, Remke, Janet, Zimmer, Karina, Zeug, Andre, Ponimaskin, Evgeni, Schmiedl, Andreas, Yin, Xiaoke, Mayr, Manuel, Halder, Rashi, Fischer, Andre, Engelhardt, Stefan, Wei, Yuanyuan, Schober, Andreas, Fiedler, Jan, and Thum, Thomas

Subjects
MicroRNA -- Properties, Fibroblasts -- Genetic aspects, Heart enlargement -- Diagnosis, Health care industry
Abstract

In response to stress, the heart undergoes extensive cardiac remodeling that results in cardiac fibrosis and pathological growth of cardiomyocytes (hypertrophy), which contribute to heart failure. Alterations in microRNA (miRNA) levels are associated with dysfunctional gene expression profiles associated with many cardiovascular disease conditions; however, miRNAs have emerged recently as paracrine signaling mediators. Thus, we investigated a potential paracrine miRNA crosstalk between cardiac fibroblasts and cardiomyocytes and found that cardiac fibroblasts secrete miRNA-enriched exosomes. Surprisingly, evaluation of the miRNA content of cardiac fibroblast-derived exosomes revealed a relatively high abundance of many miRNA passenger strands ('star' miRNAs), which normally undergo intracellular degradation. Using confocal imaging and coculture assays, we identified fibroblast exosomal-derived miR-21_3p ([miR-21.sup.*]) as a potent paracrine-acting RNA molecule that induces cardiomyocyte hypertrophy. Proteome profiling identified sorbin and SH3 domain-containing protein 2 (SORBS2) and PDZ and LIM domain 5 (PDLIM5) as [miR-21.sup.*] targets, and silencing SORBS2 or PDLIM5 in cardiomyocytes induced hypertrophy. Pharmacological inhibition of [miR-21.sup.*] in a mouse model of Ang II-induced cardiac hypertrophy attenuated pathology. These findings demonstrate that cardiac fibroblasts secrete star miRNA-enriched exosomes and identify fibroblast-derived [miR-21.sup.*] as a paracrine signaling mediator of cardiomyocyte hypertrophy that has potential as a therapeutic target., Introduction Cardiac remodeling is a hallmark in the progression of many cardiovascular diseases and is characterized by cardiomyocyte hypertrophy and cardiac fibrosis that lead often to heart failure, a major [...]

Published
2014
Full Text
View/download PDF

18. Integrative Bioinformatic Analyses of Global Transcriptome Data Decipher Novel Molecular Insights into Cardiac Anti-Fibrotic Therapies

Author

Fuchs, Maximilian, Kreutzer, Fabian Philipp, Kapsner, Lorenz A., Mitzka, Saskia, Just, Annette, Perbellini, Filippo, Terracciano, Cesare M., Xiao, Ke, Geffers, Robert, Bogdan, Christian, Prokosch, Hans-Ulrich, Fiedler, Jan, Thum, Thomas, Kunz, Meik, and HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.

Subjects
algorithm, Gene Expression Profiling, cardiac fibrosis, integrative bioinformatics, Computational Biology, web application, Fibrosis, Article, Workflow, lcsh:Chemistry, MicroRNAs, transcriptomics, lcsh:Biology (General), lcsh:QD1-999, big data, miRNAs, natural compounds, Humans, Gene Regulatory Networks, ddc:610, RNA, Messenger, Transcriptome, lcsh:QH301-705.5
Abstract

Integrative bioinformatics is an emerging field in the big data era, offering a steadily increasing number of algorithms and analysis tools. However, for researchers in experimental life sciences it is often difficult to follow and properly apply the bioinformatical methods in order to unravel the complexity and systemic effects of omics data. Here, we present an integrative bioinformatics pipeline to decipher crucial biological insights from global transcriptome profiling data to validate innovative therapeutics. It is available as a web application for an interactive and simplified analysis without the need for programming skills or deep bioinformatics background. The approach was applied to an ex vivo cardiac model treated with natural anti-fibrotic compounds and we obtained new mechanistic insights into their anti-fibrotic action and molecular interplay with miRNAs in cardiac fibrosis. Several gene pathways associated with proliferation, extracellular matrix processes and wound healing were altered, and we could identify micro (mi) RNA-21-5p and miRNA-223-3p as key molecular components related to the anti-fibrotic treatment. Importantly, our pipeline is not restricted to a specific cell type or disease and can be broadly applied to better understand the unprecedented level of complexity in big data research.

Published
2020
Full Text
View/download PDF

25. MicroRNA-Based Therapy of GATA2-Deficient Vascular Disease

Author

Hartmann, Dorothee, primary, Fiedler, Jan, additional, Sonnenschein, Kristina, additional, Just, Annette, additional, Pfanne, Angelika, additional, Zimmer, Karina, additional, Remke, Janet, additional, Foinquinos, Ariana, additional, Butzlaff, Malte, additional, Schimmel, Katharina, additional, Maegdefessel, Lars, additional, Hilfiker-Kleiner, Denise, additional, Lachmann, Nico, additional, Schober, Andreas, additional, Froese, Natali, additional, Heineke, Jörg, additional, Bauersachs, Johann, additional, Batkai, Sandor, additional, and Thum, Thomas, additional

Published
2016
Full Text
View/download PDF

27. Long noncoding RNA Chast promotes cardiac remodeling

Author

Viereck, Janika, primary, Kumarswamy, Regalla, additional, Foinquinos, Ariana, additional, Xiao, Ke, additional, Avramopoulos, Petros, additional, Kunz, Meik, additional, Dittrich, Marcus, additional, Maetzig, Tobias, additional, Zimmer, Karina, additional, Remke, Janet, additional, Just, Annette, additional, Fendrich, Jasmin, additional, Scherf, Kristian, additional, Bolesani, Emiliano, additional, Schambach, Axel, additional, Weidemann, Frank, additional, Zweigerdt, Robert, additional, de Windt, Leon J., additional, Engelhardt, Stefan, additional, Dandekar, Thomas, additional, Batkai, Sandor, additional, and Thum, Thomas, additional

Published
2016
Full Text
View/download PDF

28. Development of Long Noncoding RNA-Based Strategies to Modulate Tissue Vascularization

Author

Fiedler, Jan, primary, Breckwoldt, Kaja, additional, Remmele, Christian W., additional, Hartmann, Dorothee, additional, Dittrich, Marcus, additional, Pfanne, Angelika, additional, Just, Annette, additional, Xiao, Ke, additional, Kunz, Meik, additional, Müller, Tobias, additional, Hansen, Arne, additional, Geffers, Robert, additional, Dandekar, Thomas, additional, Eschenhagen, Thomas, additional, and Thum, Thomas, additional

Published
2015
Full Text
View/download PDF

29. MicroRNA-Based Therapy of GATA 2-Deficient Vascular Disease.

Author

Hartmann, Dorothee, Fiedler, Jan, Sonnenschein, Kristina, Just, Annette, Pfanne, Angelika, Zimmer, Karina, Remke, Janet, Foinquinos, Ariana, Butzlaff, Malte, Schimmel, Katharina, Maegdefessel, Lars, Hilfiker-Kleiner, Denise, Lachmann, Nico, Schober, Andreas, Froese, Natali, Heineke, Jörg, Bauersachs, Johann, Batkai, Sandor, and Thum, Thomas

Published
2016
Full Text
View/download PDF

30. Functional MicroRNA Library Screening Identifies the HypoxaMiR MiR-24 as a Potent Regulator of Smooth Muscle Cell Proliferation and Vascularization

Author

Fiedler, Jan, primary, Stöhr, Andrea, additional, Gupta, Shashi Kumar, additional, Hartmann, Dorothee, additional, Holzmann, Angelika, additional, Just, Annette, additional, Hansen, Arne, additional, Hilfiker-Kleiner, Denise, additional, Eschenhagen, Thomas, additional, and Thum, Thomas, additional

Published
2014
Full Text
View/download PDF

32. Long noncoding RNA Chastpromotes cardiac remodeling

Author

Viereck, Janika, Kumarswamy, Regalla, Foinquinos, Ariana, Xiao, Ke, Avramopoulos, Petros, Kunz, Meik, Dittrich, Marcus, Maetzig, Tobias, Zimmer, Karina, Remke, Janet, Just, Annette, Fendrich, Jasmin, Scherf, Kristian, Bolesani, Emiliano, Schambach, Axel, Weidemann, Frank, Zweigerdt, Robert, de Windt, Leon J., Engelhardt, Stefan, Dandekar, Thomas, Batkai, Sandor, and Thum, Thomas

Abstract

Inhibition of the long noncoding RNA Chastprevents pressure overload–induced cardiac remodeling in mice.

Published
2016
Full Text
View/download PDF

33. Cardiac endurance training alters plasma profiles of circular RNA MBOAT2.

Author

Meinecke A, Mitzka S, Just A, Cushman S, Stojanović SD, Xiao K, Mooren FC, Fiedler J, and Thum T

Subjects
1-Acylglycerol-3-Phosphate O-Acyltransferase metabolism, Adaptation, Physiological, Adult, Biomarkers blood, Cardiorespiratory Fitness, Humans, Male, Middle Aged, RNA Stability, 1-Acylglycerol-3-Phosphate O-Acyltransferase genetics, Cell-Free Nucleic Acids blood, Endurance Training methods, RNA, Circular blood
Abstract

Marathon running is an extreme physical activity, which determines cardiopulmonary adaption of athletes. Circular RNAs (circRNAs) as potential biomarkers in the blood stream have so far not been tested after such strenuous activities. In silico approaches were performed to identify the potential candidate circRNA MBOAT2. Next, we demonstrated high stability and conservation of circRNA MBOAT2 as well as its abundancy in human plasma. In addition to Sanger sequencing of the circRNA specific head-to-tail junction, or back-splice site, we established a synthetic plasmid standard which allowed exact copy number calculations of circRNA MBOAT2. We then analyzed plasmatic circRNA MBOAT2 and observed a significantly lower level 24 h after the marathon. Such alterations were correlated to physical exercise parameters confirming the role of circRNA MBOAT2 as a promising noncoding RNA biomarker detecting cardiopulmonary adaption. NEW & NOTEWORTHY In brief, we herein report a timeline of circulating circular RNA (circRNA) MBOAT2 in a cohort of marathon runners. Time-course analysis of plasmatic circRNA MBOAT2 demonstrated a significantly lowered level 24 h after the marathon. Abundancy of circRNA was correlated to physical exercise parameters highlighting the role of circRNA MBOAT2 as a valuable noncoding RNA biomarker detecting and following up cardiopulmonary adaption.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results