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The emergence of Alpha, Beta, Gamma, Delta, and Omicron variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for several million deaths up to now. Because of the huge amount of vaccine escape mutations in the spike (S) protein for different variants, the design of material for combating SARS-CoV-2 is very important for our society. Herein, we report on the design of a human angiotensin converting enzyme 2 (ACE2) peptide-conjugated plasmonic-magnetic heterostructure, which has the capability for magnetic separation, identification via surface enhanced Raman spectroscopy (SERS), and inhibition of different variant SARS-CoV-2 infections. In this work, plasmonic-magnetic heterostructures were developed using the initial synthesis of polyethylenimine (PEI)-coated Fe

Additive manufacturing (AM) is a new and promising production methodology adept at producing complex geometries, which can be optimized for lower weight and enhanced capabilities. The material properties of these additive components are dictated by the microstructures developed during processing, with a high sensitivity to grain structure and associated anisotropy. With this new processing modality comes the added difficulty of understanding the thermodynamics and kinetic mechanisms that dictate the evolution of microstructure. This research addresses the unique thermal conditions present in AM and the pathways for grain refinement in nanofunctionalized aluminum alloys. The Al-Ta system, in which Al3Ta intermetallic compounds are demonstrated to have substantial grain refining capacity, are the focus of this study. The grain size is shown to be reduced relative to pure aluminum by 1000X when tantalum is added at 1 vol%. The effectiveness of the Al3Ta intermetallic is dictated by the crystallography and availability of the inoculant phase under AM conditions.

A novel, alloy-agnostic, nanofunctionalization process has been utilized to produce metal matrix composites (MMCs) via additive manufacturing, providing new geometric freedom for MMC design. MMCs were produced with the addition of tungsten carbide nanoparticles to commercially available AISMOMg alloy powder. Tungsten carbide was chosen due to the potential for coherent crystallographic phases that were identified utilizing a lattice-matching approach to promote wetting and increase dislocation interactions. Structures were produced with evenly distributed strengthening phases leading to tensile strengths > 385 MPa and a 50% decrease in wear rate over the commercially available AISMOMg alloy at only 1 vol% loading of tungsten carbide.

Metal-based additive manufacturing, or three-dimensional (3D) printing, is a potentially disruptive technology across multiple industries, including the aerospace, biomedical and automotive industries. Building up metal components layer by layer increases design freedom and manufacturing flexibility, thereby enabling complex geometries, increased product customization and shorter time to market, while eliminating traditional economy-of-scale constraints. However, currently only a few alloys, the most relevant being AlSi10Mg, TiAl6V4, CoCr and Inconel 718, can be reliably printed; the vast majority of the more than 5,500 alloys in use today cannot be additively manufactured because the melting and solidification dynamics during the printing process lead to intolerable microstructures with large columnar grains and periodic cracks. Here we demonstrate that these issues can be resolved by introducing nanoparticles of nucleants that control solidification during additive manufacturing. We selected the nucleants on the basis of crystallographic information and assembled them onto 7075 and 6061 series aluminium alloy powders. After functionalization with the nucleants, we found that these high-strength aluminium alloys, which were previously incompatible with additive manufacturing, could be processed successfully using selective laser melting. Crack-free, equiaxed (that is, with grains roughly equal in length, width and height), fine-grained microstructures were achieved, resulting in material strengths comparable to that of wrought material. Our approach to metal-based additive manufacturing is applicable to a wide range of alloys and can be implemented using a range of additive machines. It thus provides a foundation for broad industrial applicability, including where electron-beam melting or directed-energy-deposition techniques are used instead of selective laser melting, and will enable additive manufacturing of other alloy systems, such as non-weldable nickel superalloys and intermetallics. Furthermore, this technology could be used in conventional processing such as in joining, casting and injection moulding, in which solidification cracking and hot tearing are also common issues.

Tumors are complex ecosystems composed of networks of interacting 'normal' and malignant cells. It is well recognized that cytokine-mediated cross-talk between normal stromal cells, including cancer-associated fibroblasts (CAFs), vascular endothelial cells, immune cells, and cancer cells, influences all aspects of tumor biology. Here we demonstrate that the cross-talk between CAFs and cancer cells leads to enhanced growth of oncolytic virus (OV)-based therapeutics. Transforming growth factor-β (TGF-β) produced by tumor cells reprogrammed CAFs, dampened their steady-state level of antiviral transcripts and rendered them sensitive to virus infection. In turn, CAFs produced high levels of fibroblast growth factor 2 (FGF2), initiating a signaling cascade in cancer cells that reduced retinoic acid-inducible gene I (RIG-I) expression and impeded the ability of malignant cells to detect and respond to virus. In xenografts derived from individuals with pancreatic cancer, the expression of FGF2 correlated with the susceptibility of the cancer cells to OV infection, and local application of FGF2 to resistant tumor samples sensitized them to virotherapy both in vitro and in vivo. An OV engineered to express FGF2 was safe in tumor-bearing mice, showed improved therapeutic efficacy compared to parental virus and merits consideration for clinical testing.

Hypothesis Ligand exchange is a widely-used method of controlling the surface chemistry of nanomaterials. Exchange is dependent on many factors including the age of the core particle being modified. Aging of the particles can impact surface structure and composition, which in turn can affect ligand binding. Experiments To quantify the effects of aging on ligand exchange, we employed a technique to track the exchange of radiolabeled 14C-oleic acid with unlabeled, oleic acid bound to iron oxide nanoparticles. Liquid scintillation counting (LSC) was used to determine the amount of 14C-oleic acid adsorbing to the particles throughout the duration of the exchange for particles aged for 2 days, 7 days, and 30 days. Findings Results revealed an increase in the total amount of ligands exchanged with aging up to 30 days. Kinetic analysis of these results revealed a significant decrease in the overall rate of ligand exchange between 2 and 30 days. The change in extent of adsorption with age could suggest increased availability of free binding sites. A follow-up study comparing exchange with oxidized and unoxidized particles suggested this increase in ligand adsorption may be due to changes in the Fe2+/Fe3+ ratio on the surface as the particles aged.

In a laboratory ultimatum bargaining experiment, responder behavior is more consistent with game-theoretic predictions when responders indicate a binding minimum acceptable offer (MAO) or when rejection penalizes a “hostage” third player. In general, female subjects indicated higher MAOs but had a much greater reaction than males to the presence of a third player. Offers increase in the presence of a binding MAO but are not affected by a third player. Behavior in our experiment is also generally consistent with hypotheses based on a popular personality test instrument.

This paper uses responder pre-commitment and psychological type, as measured by the Myers- Briggs Type Indicator (MBTI), to gain insight into subject behavior in a laboratory ultimatum bargaining experiment. Three experiment design details are noteworthy: (1) one design requires responders to make a nonbinding pre-commitment rejection level prior to seeing the offer, (2) one design requires responders to make a binding pre-commitment rejection level, and (3) one design includes a third person (or “hostage”) who makes no decision, but whose payment depends on the proposal being accepted. Offers are higher when proposers know that responders make a binding pre-commitment to reject but are not different when a hostage is present. Responders make lower pre-commitments when they are binding and when a hostage is present. Behavior in our experiment is generally consistent with hypotheses based on theoretical underpinnings of the MBTI and its descriptions of psychological type.

This paper uses responder pre-commitment and the Jungian theory of mental activity and psychological type, as measured by the widely-used Myers-Briggs Type Indicator (MBTI), to gain insight into subject behavior in a laboratory ultimatum bargaining experiment. Three experiment design details are noteworthy: (1) one design requires responders to make a nonbinding pre-commitment rejection level prior to seeing the offer, (2) one design requires responders to make a binding pre-commitment rejection level, and (3) one design includes a third person (or “hostage”) who makes no decision, but whose payment depends on the proposal being accepted. In general, we find behavior in our experiment to be consistent with hypotheses based on theoretical underpinnings of the MBTI and its descriptions of psychological type.