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Abstract A series of treatments were designed to evaluate the efficacy of feeding commercial multispecies probiotics feeding with enzymes and fermentation process on the growth parameters and culture environment of Pacific white shrimp, Litopenaeus vannamei (Boone, 1931), in an intensive culture system. Commercial multispecies probiotics and enzymes (PEs) were continuously applied in three different doses, namely (i) 0.2, (ii) 0.4, and (iii) 0.6 mg L−1 and designated as 0.2, 0.4, and 0.6 PE during the first 30 days of intensive culture of Pacific white shrimp, L. vannamei (Boone, 1931). The probiotics were continually applied every alternate day, while the enzymes were added every sixth day throughout the trial period. The PE dose for all treated tanks was increased by 0.2 ppm after 30 days of culture and another 0.2 ppm after day 60. Meanwhile, fermentation technique, which has become the common method applied in Indonesia in the control treatment, was added on the same day with the PE group with increasing dosage, following the same trend with the 0.6 PE group. Results showed that the group receiving 0.6 PE showed higher final biomass, higher mean weight, and protein retention efficiency as well as a lower feed conversion ratio compared with the control treatment. Abundance of Vibrio spp. remained below 103 cfu mL−1 throughout the trial. Water quality indicators TAN, NO2N and NO3N peaked in weeks 3–5 and then declined after that until the end of the culture period in all tanks. This decline was significantly faster in PE‐treated tanks. Multispecies PEs have potential applications in controlling Vibrio spp., maintaining proper water quality condition, and enhancing the growth of shrimp in intensive culture system.

Abstract Plant‐derived products have been suggested to partially replace fishmeal in aquafeed; however, often at decreased growth performance, inflammatory responses, and increased susceptibility to diseases. This study assessed the effects of the commercial phytogenic feed additive Digestarom PEP MGE on the growth, nutritional performance, and immune response of L. vannamei. Juvenile shrimp (N = 540) were stocked in 36 tanks (V = 100 L) for 63 days and fed one of the four experimental diets: (i) standard formulation (control, 24% fishmeal), (ii) low fishmeal diet (5%), (iii) low fishmeal diet plus 0.2 g/kg Digestarom PEP MGE, and (iv) low fishmeal diet plus 0.4 g/kg Digestarom PEP MGE. The results obtained after 63 days of feed supplementation suggest that the blend of essential oils tested compensated for the negative performance and health consequences of the low fishmeal diet. Particularly, the survival, FCR, total hemocyte count, and respiratory burst of the shrimp fed a low fishmeal diet supplemented with this phytogenic improved up to the levels recorded for shrimp fed a high fishmeal diet. Overall, results suggest that Digestarom PEP MGE can be incorporated into shrimp low fishmeal diets to compensate for the negative performance and immunological effects of partially replacing fishmeal with plant‐based protein.