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In the face of mounting environmental issues, people around the world are reporting the experience of difficult emotions such as anxiety and worry, or what is increasingly referred to as eco-anxiety. It is often acknowledged that symptoms of eco-anxiety can range in severity or fall along a continuum. Such a proposition has important implications, as it may help to explain why some forms of eco-anxiety are more mal(adaptive) than others. In five studies (Total N = 2939) across three countries (Canada, China, United Kingdom), we examined how measures that may encompass a continuum of environment-related worry and anxiety were associated with each other and with measures of environmental concern, an older concept that may capture the less severe end of eco-anxiety responses. We also explored if these various measures were differentially linked to aspects of mental health and a pro-environmental orientation. Results revealed that measures of eco-anxiety and environmental concern were often moderately-strongly correlated. Eco-anxiety measures exhibited relatively consistent relationships with greater ill-being but mixed relationships with indices of well-being. There was some evidence of more severe eco-anxiety measures being associated with poorer mental health and environmental concern measures being associated with better mental health. Measures of both eco-anxiety and environmental concern evidenced larger and more consistent relationships with indices of a pro-environmental orientation, with the most severe eco-anxiety measure exhibiting some notably weaker relationships. Together, the present work provides preliminary insights into the nomological network of the continuum of eco-anxiety responses and its integration into future work on eco-anxiety.

The extent to which morality and being a moral person are important to one's identity is most commonly assessed using Aquino and Reed's (2002) Self-Importance of Moral Identity Scale (SIMIS). This study provided detailed psychometric examinations of the structure and discrimination levels of the SIMIS in a large (N = 2108) and heterogeneous sample. Results indicated that the SIMIS is clearly 2-dimensional, as expected. The Internalization and Symbolization subscales provided sufficient, and sometimes high levels of test information across the latent trait continuums. There were no redundant items and no bias based on gender. The most notable, albeit minor, shortcomings were that there are too many response options and that test information (discrimination power) was diminished at high levels of the Internalization latent trait continuum, apparently due to skewness. The fluctuating levels of measurement precision resulted in slightly greater attenuations in effect sizes for Internalization than for Symbolization across data for 31 other measures. The present findings from a large dataset and a variety of modern, revealing statistical methods provided relatively consistent, favorable findings for the measure.

The interfacial phenomena behind analyte separation in a reversed-phase liquid chromatography column take place nearly exclusively inside the silica mesopores. Their cylindrical geometry can be expected to shape the properties of the chromatographic interface with consequences for the analyte density distribution and diffusivity. To investigate this topic through molecular dynamics simulations, we introduce a cylindrical pore inside a slit pore configuration, where the inner curved and outer planar silica surface bear the same bonded phase. The present model replicates an average-sized (9 nm) mesopore in an endcapped C18 column equilibrated with a mobile phase of 70/30 (v/v) water/acetonitrile. Simulations performed for ethylbenzene and acetophenone show that the surface curvature shifts the bonded phase and analyte density toward the pore center, decreases the solvent density in the bonded-phase region, increases the acetonitrile excess in the interfacial region, and considerably enhances the surface diffusivity of both analytes. Overall, the cylindrical pore provides a more hydrophobic environment than the slit pore. Ethylbenzene density is decidedly increased in the cylindrical pore, whereas acetophenone density is nearly equally distributed between the cylindrical and slit pore. The cylindrical pore geometry thus sharpens the discrimination between the apolar and moderately polar analytes while enhancing the mass transport of both.

Alopecia occurs frequently in captive populations of nonhuman primates. Because multiple factors can play a role in alopecia, a better understanding of its etiology will help identify potential welfare concerns. The purpose of this study was to investigate risk factors for alopecia in a breeding colony of baboons with a focus on pregnancy and age. Alopecia was scored on a scale of 0 (no alopecia) to 5 (severe alopecia) in 253 female baboons during routine physicals. The subjects ranged in age from 4 to 23 y (Mean = 9.6) and were categorized as pregnant (n = 83), nursing (n = 60) or control (n = 110). Resulting alopecia scores were combined into 2 categories (mild = 0 or 1; moderate = 2 or 3); no animals scored a 4 or 5. Significantly more pregnant females had moderate alopecia than did control females. There was no effect of age on alopecia. An unexpected outcome was that among nursing females, more of those with female infants had moderate alopecia than did those with male infants. The impact of the infant's sex on alopecia may be due to sex differences in maternal contact or maternal investment. This information adds to our understanding of alopecia risk factors in captive nonhuman primates.

Rationale: Pulmonary arterial hypertension (PAH) is characterized by structural remodeling of pulmonary arteries and arterioles. Underlying biological processes are likely reflected in a perturbation of circulating proteins. Objectives: To quantify and analyze the plasma proteome of patients with PAH using inherited genetic variation to inform on underlying molecular drivers. Methods: An aptamer-based assay was used to measure plasma proteins in 357 patients with idiopathic or heritable PAH, 103 healthy volunteers, and 23 relatives of patients with PAH. In discovery and replication subgroups, the plasma proteomes of PAH and healthy individuals were compared, and the relationship to transplantation-free survival in PAH was determined. To examine causal relationships to PAH, protein quantitative trait loci (pQTL) that influenced protein levels in the patient population were used as instruments for Mendelian randomization (MR) analysis. Measurements and Main Results: From 4,152 annotated plasma proteins, levels of 208 differed between patients with PAH and healthy subjects, and 49 predicted long-term survival. MR based on cis-pQTL located in proximity to the encoding gene for proteins that were prognostic and distinguished PAH from health estimated an adverse effect for higher levels of netrin-4 (odds ratio [OR], 1.55; 95% confidence interval [CI], 1.16-2.08) and a protective effect for higher levels of thrombospondin-2 (OR, 0.83; 95% CI, 0.74-0.94) on PAH. Both proteins tracked the development of PAH in previously healthy relatives and changes in thrombospondin-2 associated with pulmonary arterial pressure at disease onset. Conclusions: Integrated analysis of the plasma proteome and genome implicates two secreted matrix-binding proteins, netrin-4 and thrombospondin-2, in the pathobiology of PAH.

The present study sought to examine: (1) how the components of authenticity (i.e., authentic living, self-alienation, accepting external influence) relate to one another at between- and within-person levels of analysis; (2) how the authenticity facets relate to meaning in life (i.e., purpose, comprehension, mattering) and life satisfaction at these levels of analysis; and (3) whether these relationships persist when controlling for affect and self-esteem.Canadian undergraduates (N = 203) completed a trait questionnaire and end-of-day reports on these constructs for two weeks (n = 2335).At between- and within-person levels, authentic living was negatively associated with self-alienation and accepting external influence, while the latter two facets were positively associated. Authentic living was positively related to well-being and predicted greater well-being the following day. Alternatively, self-alienation and accepting external influence were negatively related to well-being, and self-alienation predicted lower well-being the following day. Relationships involving authentic living and self-alienation were more robust than those involving accepting external influence.Extending research on authenticity beyond between-person relationships, our findings show that daily states of authenticity predict well-being in nuanced ways, depending on the facet of authenticity. This highlights the importance of distinguishing levels of analyses and facets of authenticity.

Across captive settings, nonhuman primates may develop an array of abnormal behaviors including stereotypic and self-injurious behavior. Abnormal behavior can indicate a state of poor welfare, since it is often associated with a suboptimal environment. However, this may not always be the case as some behaviors can develop independently of any psychological distress, be triggered in environments known to promote welfare, and be part of an animal's coping mechanism. Furthermore, not all animals develop abnormal behavior, which has led researchers to assess risk factors that differentiate individuals in the display of these behaviors. Intrinsic risk factors that have been identified include the animal's species and genetics, age, sex, temperament, and clinical condition, while environmental risk factors include variables such as the animal's rearing, housing condition, husbandry procedures, and research experiences. To identify specific triggers and at-risk animals, the expression of abnormal behavior in captive nonhuman primates should be routinely addressed in a consistent manner by appropriately trained staff. Which behaviors to assess, what assessment methods to use, which primates to monitor, and the aims of data collection should all be identified before proceeding to an intervention and/or treatment. This article provides guidance for this process, by presenting an overview of known triggers and risk factors that should be considered, steps to design a comprehensive evaluation plan, and strategies that might be used for prevention or treatment. It also outlines the tools and processes for assessing and evaluating behavior in an appendix. This process will lead to a better understanding of abnormal behavior in captive primate colonies and ultimately to improved welfare.

Modelling epidemics via classical population-based models suffers from shortcomings that so-called individual-based models are able to overcome, as they are able to take heterogeneity features into account, such as super-spreaders, and describe the dynamics involved in small clusters. In return, such models often involve large graphs which are expensive to simulate and difficult to optimize, both in theory and in practice. By combining the reinforcement learning philosophy with reduced models, we propose a numerical approach to determine optimal health policies for a stochastic epidemiological graph-model taking into account super-spreaders. More precisely, we introduce a deterministic reduced population-based model involving a neural network, and use it to derive optimal health policies through an optimal control approach. It is meant to faithfully mimic the local dynamics of the original, more complex, graph-model. Roughly speaking, this is achieved by sequentially training the network until an optimal control strategy for the corresponding reduced model manages to equally well contain the epidemic when simulated on the graph-model. After describing the practical implementation of this approach, we will discuss the range of applicability of the reduced model and to what extent the estimated control strategies could provide useful qualitative information to health authorities., Comment: 37 pages, 21 figures, to be published in the journal "Optimal Control Applications and Methods" (Special Issue: Optimal control in therapeutics and epidemiology)

Background and goal The incidence of nonalcoholic fatty liver disease (NAFLD)-associated hepatocellular carcinoma (HCC) is rising. We aimed to characterize risk factors for NAFLD-HCC development. Methods We performed a retrospective case-control study of HCC cases from a cohort of NAFLD patients who underwent at least 2 computed tomography scans. NAFLD-HCC cases confirmed on contrast imaging and/or biopsy were included. Controls were NAFLD patients without HCC matched by sex and age. Clinical variables were assessed. Visceral adipose tissue and subcutaneous adipose tissue were measured by computed tomography at 2 timepoints: before HCC diagnosis and at diagnosis. Results We identified 102 subjects [34 HCC cases, 68 controls, 65% (n=66) males, mean age: 69 y] from 2002 to 2016. Cirrhosis was present in 91%. In multivariate analysis, statin use was protective against HCC [odds ratio (OR)=0.20, 95% confidence interval (CI): 0.07-0.60, P=0.004], while hypertension was a risk factor for HCC (OR=5.80, 95% CI: 2.01-16.75, P=0.001). In multivariate analysis, visceral adipose tissue in males was higher before HCC diagnosis and declined by HCC diagnosis in 86%, which was a significant difference compared with controls (OR=2.78, 95% CI: 1.10-7.44, P=0.04). Conclusions In a cohort of NAFLD-HCC patients, statin use was protective against HCC, while hypertension conferred an increased risk. Visceral adiposity at baseline was not a risk factor, but was higher in male patients before HCC development, declining in the majority by HCC diagnosis.

Aedes aegypti have a wide variety of sensory pathways that have supported success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. Investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. Numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. In particular, compared to that of the adult form, there has been a lack of studies directed towards the immature life stages. Additionally, although numerous studies have pinpointed specific sensory receptors as well as relevant response behaviors, there has been a lack of studies able to monitor both concurrently. To begin filling aforementioned gaps, here we engineered Ae. aegypti to ubiquitously express a genetically encoded calcium indicator, GCaMP6s. Using this strain, combined with advanced confocal microscopy, we were able to simultaneously measure live stimulus-evoked calcium responses in both neuronal and muscle cells with a wide spatial range and resolution. Moreover, by coupling in vivo calcium imaging with behavioral assays we were able to gain functional insights into how stimulus-evoked neural and muscle activities are represented, modulated, and transformed in mosquito larvae enabling us to elucidate mosquito sensorimotor properties important for life-history-specific foraging strategies.Significance StatementUnderstanding mosquito sensory systems and resulting behavior has been a major factor in the advancement of mosquito control innovations. Aedes aegypti larvae offer an effective life stage for further elucidating information on mosquito sensory systems. Due to their relatively simplified nervous system, mosquito larvae are ideal for studying neural signal transduction, coding, and behavior. Moreover, a better understanding of the larval sensory system may enable the development of novel control methodologies able to target mosquitoes before they reach a vector-competent stage. Here we engineer Ae. aegypti to ubiquitously express a genetically encoded calcium indicator, GCaMP6s and use this tool to observe links between sensorimotor responses and behavior by exploiting live calcium imaging as well as live tracking based behavioral assays.

Hair cortisol concentrations (HCCs) are measures of long-term hypothalamic-pituitary-adrenocortical (HPA) activity and can be used as indicators of chronic stress. However, intrinsic factors such as an animal’s age and sex can also have an impact on resulting HCCs. Although baboons are commonly studied in captivity, little is known about baseline HCC in this population. Here we measured HCC in two same-sex groups of captive olive (Papio hamadryas anubis) baboons and olive/yellow baboon (Papio hamadryas cynocephalus) crosses housed in large outdoor corrals, and we assessed the impact of age and sex on HCC as major variables of interest. Hair was gently shaved from the back of the neck when the animals were sedated for routine physicals. Subjects were divided into three age categories: juvenile (2–4 years), adult (9–12 years), and senior (13–19 years). The “senior” category contained only males. Results confirm an effect of sex and age on HCCs. Females had higher levels of hair cortisol than males, and juveniles had higher levels than adults. There was also a significant sex × age interaction. There were no sex differences in HCCs in juveniles, but there was a greater decline in HCCs in adult males than in adult females. Within males, there was a significant difference in levels of hair cortisol across the three age categories. Juveniles had higher levels than did adults and seniors, but adults and seniors were not significantly different from one another. These results provide baseline measures of hair cortisol in captive baboons and demonstrate effects of sex and age on HCCs.

We asked Eleanor Lutz for an interview upon investigating work she has shared through her Tabletop Whale blog (tabletopwhale.com/). On her blog, Eleanor shares her love of design as applied to the visualization of public datasets. She provides examples and the processes by which her stunning graphics come to fruition, as well as code examples in a notebook format that are useful as a service to the graphics community and her potential collaborators. As we prepared for the interview, we became more and more enamored with her Atlas of Space visualizations—many of which she designed specifically to be printed as wall posters. In this article, we share the insights she shared with us during the interview.

Abnormal behavior occurs in a number of captive nonhuman primate species and is often used as an indicator of welfare. However, reported levels of abnormal behavior often vary across species, making general welfare judgments difficult. The purpose of this study was to assess differences in levels of abnormal behavior and associated risk factors across three species of Old World monkeys in order to identify similarities and differences across species. The subjects were 415 (109 females) cynomolgus macaques (Macaca fascicularis), 365 (181 females) rhesus macaques (Macaca mulatta), and 331 (187 females) baboons (Papio hamadryas) that had been singly-housed for 30–120 days. A 5-min observation using one-zero sampling recorded the presence or absence of abnormal behavior for each animal. Macaques exhibited higher levels of abnormal behavior than baboons (29% vs. 14%; χ2(1) = 24.849, p < 0.001), but there was no difference between macaque species (30% vs. 28%; χ2(1) = 0.263, p = 0.608). Risk factors also varied. Overall, males exhibited greater levels of motor stereotypies (b = 0.425, p < 0.05), females greater levels of abnormal appetitive behavior (b = 1.703, p < 0.05), and older animals greater levels of self-directed behavior (b = 0.065, p < 0.05). However, macaques exhibited greater levels of motor stereotypy (b = 2.527, p < 0.001) and self-directed behavior (b = 2.968, p < 0.005) than did baboons. There was also a genus × sex interaction for abnormal appetitive behavior (b = −2.379, p < 0.01) and a genus × age interaction for motor stereotypy (b = −0.167, p < 0.05). These results demonstrate that differences in abnormal behavior exist across closely-related primate species. Therefore, a single species cannot be used generally as a model for abnormal behavior or animal welfare.

Mosquitoes are prolific disease vectors that affect public health around the world. Although many studies have investigated search strategies used by host-seeking adult mosquitoes, little is known about larval search behavior. Larval behavior affects adult body size and fecundity, and thus the capacity of individual mosquitoes to find hosts and transmit disease. Understanding vector survival at all life stages is crucial for improving disease control. In this study we use experimental and computational methods to investigate the chemical ecology and search behavior ofAedes aegyptimosquito larvae. We show that larvae do not respond to several olfactory cues used by adultAe. aegyptito assess larval habitat quality, but perceive microbial RNA as a potent foraging attractant. Second, we demonstrate thatAe. aegyptilarvae use a strategy consistent with chemokinesis, rather than chemotaxis, to navigate chemical gradients. Using computational modeling, we further show that chemokinesis is more efficient than chemotaxis for avoiding repellents in ecologically relevant larval habitat sizes. Finally, we use experimental observations and computational analyses to demonstrate that larvae respond to starvation pressure by optimizing exploration behavior. Our results identify key characteristics of foraging behavior in a disease vector mosquito, including the identification of a surprising foraging attractant and an unusual behavioral mechanism for chemosensory preference. In addition to implications for better understanding and control of disease vectors, this work establishes mosquito larvae as a tractable model for chemosensory behavior and navigation.

Chimpanzees demand specialized housing and care and the highest degree of attention to animal welfare. The current project used a survey method to collate information on chimpanzee housing and behavioral indices of welfare across all 6 of the chimpanzee research facilities in the United States. Data were compiled on 701 chimpanzees ranging from 2 to 62 y old (mean age, 26.0 y). All chimpanzees except for one were socially housed; the median group size was 7 animals, and group sizes ranged from 1 to 14. All of the subjects had access to outdoor spaces each day. Daily access to a natural substrate in the chimpanzee's enclosure was available for 63.8% of the subjects. Overall, 94.1% of the chimpanzees used tools to acquire food, 48.1% built nests, 75.8% copulated, and 83.3% initiated grooming bouts. The following atypical behaviors were reported most often: rocking (13.0%), coprophagy (10.0%), and stereotyped behaviors other than rocking (9.4%). There was widespread evi- dence of positive animal training techniques, with nearly all (97.7%) subjects reported to generally voluntarily cooperate with shifting in their enclosure, and 72.2% were reported to present for an injection of anesthetic. We include some comparison between these findings and data describing zoo-housed chimpanzees. In addition, we discuss survey findings in reference to recommendations made by the NIH Working Group on the Use of Chimpanzees in NIH-supported Research. The current survey assessed a larger sample of chimpanzees living under human care than has been published previously. This broad analysis can help to guide future improvements in behavioral management to address behavioral problems or deficits.

Background Alopecia in captive non-human primates is often presented as a welfare issue. However, it is a complex condition with a number of possible causes. The purpose of this study was to assess the impact of pregnancy and hair cortisol concentrations on alopecia in rhesus macaques. Methods Subjects were 113 socially housed adult female rhesus macaques (27 pregnant, 35 nursing infants, 51 controls). During routine physicals, photographs were taken for alopecia assessment and hair samples were collected for cortisol assay. Results Alopecia was more prevalent in pregnant than in control females, but there was no association between alopecia and hair cortisol. However, there was a significant effect of pregnancy on hair cortisol. Nursing females had higher hair cortisol levels than pregnant females, which had higher levels than control females. Conclusions Although alopecia does not appear to be associated with hair cortisol, both alopecia and hair cortisol were associated with pregnancy.

Western culture has developed an obsession with happiness. At first glance, the premium placed on happiness seems warranted given that a large body of research has highlighted its benefits. Yet, emerging lines of research suggest that the outcomes of being happy are very different from the outcomes of seeking to be happy. Whereas the former is often linked to, and in some cases even causes, positive outcomes, the latter seems to do the opposite. This chapter first reviews research examining the negative effects of pursuing happiness at the individual level. We then highlight a number of important differences between Western and Eastern notions of happiness while also considering how these notions are beginning to change. Next, we examine the consequences stemming from valuing happiness at the societal level. We discuss prominent cultural emotion norms tied to happiness and the implications that these norms have for individual emotional functioning. Throughout, we note that the downsides of pursuing happiness may spread to other Eastern societies, such as the Middle East/North Africa region, through increased exposure to Western values, norms, and practices via globalization. Lastly, we highlight a number of important social determinants of happiness that can inform governments in their mandate to boost the happiness, life satisfaction, and overall wellbeing of their citizens.

Introduction: Prehospital sepsis alerts assist paramedics in identifying patients with sepsis and in communicating this diagnosis to receiving facilities. Following the prospective implementation study of our regional systemic inflammatory response syndrome-based alert criteria (Alert), the purpose of this sub-study was to determine the cause of Alert false negatives (patients without an Alert that subsequently met sepsis criteria in the Emergency Department (ED)). Additionally, the sensitivity of the Alert for detecting sepsis was compared to the Quick Sequential Organ Failure Assessment (qSOFA) and Hamilton Early Warning Score (HEWS). Methods: This study was an additional analysis of the prospective Alert implementation study. Included patients were ≥ 18 years old, transported by a regional Emergency Medical Service and met severe sepsis or septic shock criteria (SS/SS, 2012 Surviving Sepsis Guidelines) in regional EDs in 2013. False negative patients were identified prospectively and reviewed by comparing paramedic determined Alert status to the retrospective application of the Alert criteria to Paramedic Call Report (PCR) data. The Alert sensitivity was first calculated from prospective data, then retrospective sensitivities of the Alert, qSOFA and HEWS were calculated by retrospectively applying these tools to PCRs, using ED diagnosis of SS/SS as reference standard. Results: In 2013, 229 patients met SS/SS criteria in the ED and had PCRs available; 115 (50.2%) were male and median age [interquartile range] was 76.0 [63.0-84.0]. Of 229, 149 (65.0%) arrived in the ED without an Alert (false negatives) and 46 (30.9%) of these met Alert criteria retrospectively and were therefore missed by paramedics. Sensitivity of the Alert was 34.9% when applied by paramedics and 41.5% when applied retrospectively to PCRs. The retrospective sensitivities of the qSOFA and HEWS were 37.6% and 67.7%, respectively. Conclusion: In ED patients diagnosed with SS/SS who arrived with no Alert, the majority (69.1%) were missed by the Alert criteria, rather than by paramedic application of the tool. The Alert had a sensitivity of 34.9%. When applied retrospectively and compared to the Alert, qSOFA had similar sensitivity and HEWS had increased sensitivity. Future research should focus on deriving improved alerts or implementing those with higher accuracy, such as HEWS.

Introduction: Palliative Care aims to relieve suffering and improve the quality of living and dying in patients with life-limiting, progressive conditions. Many patients and families prefer to stay at home at end of life. Despite this, many access 911 in times of apparent crisis. It has been noted in the literature that a well functioning palliative care system includes considering Emergency Medical Services as part of the patients’ circle of care. Training in palliative care is traditionally limited or absent for prehospital clinicians, including Paramedics and Emergency Medical Services Physicians. Furthermore, in our region, there are currently no medical directives available to Paramedics within the 911 system specifically addressing the needs of palliative care patients. Methods: A feasibility study (Expanding Care by Paramedics for Palliative Patients – EC3P) was designed to evaluate implementation of a new palliative care medical directive with trained teams of Paramedics available to respond to 911 calls. As part of this study, a pre-implementation retrospective chart review was performed. Patient care records were screened for “palliative” within the past medical history and text fields. Information about dispatch and scene times, patient demographics, details of patient encounter, and disposition of the patient were recorded. Descriptive statics were used. Results: Data was reviewed for all calls in 2018. Call data was reviewed to exclude those that were pediatric (Conclusion: Much is unknown about the palliative patient population as it intersects with prehospital emergency care. This study will help provide information needed to guide further research and implementation.

Effective environmental enrichment is used by animals, promotes species-typical behavior, and decreases abnormal behavior. Porches attached to the front of an animal's cage provide additional space, perching opportunities, and a better view of the surroundings. Here we assessed the effectiveness of porches as a form of enrichment and identified characteristics of the animals most likely to use the porches. We videorecorded and scored the behavior of 18 (9 male, 9 female) singly housed cynomolgus macaques (Macaca fascicularis) during 3 observation intervals (15 min each) the week before, during, and after exposure to the porches. Changes in abnormal and tension-related behaviors (pacing, yawning, scratching) and species-typical behaviors were compared across the 3 wk of observation. Novel object temperament tests were performed before and after the study. During observation periods, subjects spent an average of 75% of time in the porch. No changes in pacing or tension-related behaviors occurred, but activity decreased during and after porch exposure, rest increased during the porch exposure, and consumption decreased afterward. Eight subjects were categorized as having a bold temperament, and the remaining 10 subjects had an intermediate temperament. Sex and a temperament×cage location interaction were predictors of porch usage. Males used the porches more than did females, and those with an intermediate temperament were less likely to use the porches when they were located in the lower cages. Porches are beneficial in that they are used for extensive periods of time, but the benefits can vary according to the individual animal.

Hydraulic fracturing (fracking) poses significant threats to freshwater resources and stream ecosystems. Little research exists to quantify the ecological impact and in Pennsylvania alone over 10,000 wells have been permitted. This study aimed to determine if hydraulic fracturing is having any impacts on stream ecosystem health by measuring stream pH and temperature, macroinvertebrate index of biological integrity (IBI), and the gill morphology of individuals in the Hydropsychidae Diplectrona taxa. Six streams in northwestern Pennsylvania were selected as study sites (three with fracking occurring in their watershed and three without fracking). IBI scores were significantly higher at non-fracked sites and were also correlated with stream pH. Macroinvertebrate gill width did not vary between fracked and non-fracked sites but was correlated with percent hydric soils, suggesting that hydric soils may be a good long-term indicator of stream dissolved oxygen. While our results did not indicate differences in H...

We present Herschel PACS observations of the [C II] 158 μm emission line in a sample of 24 intermediate mass (9 < log M_*/M_⊙ < 10) and low metallicity (0.4 < Z/Z_⊙ < 1.0) galaxies from the xCOLD GASS survey. In combination with IRAM CO (1−0) measurements, we establish scaling relations between integrated and molecular region L_([CII])/L_(CO (1–0)) ratios as a function of integrated galaxy properties. A Bayesian analysis reveals that only two parameters, metallicity and offset from the main sequence, Δ(MS), are needed to quantify variations in the luminosity ratio; metallicity describes the total dust content available to shield CO from UV radiation, while Δ(MS) describes the strength of this radiation field. We connect the L_([CII])/L_(CO (1–0)) ratio to the CO-to-H_2 conversion factor and find a multivariate conversion function, which can be used up to z ∼ 2.5. This function depends primarily on metallicity, with a second-order dependence on Δ(MS). We apply this to the full xCOLD GASS and PHIBSS1 surveys and investigate molecular gas scaling relations. We find a flattening of the relation between gas mass fraction and stellar mass at log M_* < 10.0. While the molecular gas depletion time varies with sSFR, it is mostly independent of mass, indicating that the low L_(CO)/SFR ratios long observed in low-mass galaxies are entirely due to photodissociation of CO and not to an enhanced star formation efficiency.

Covalent binding of [7-3H]styrene (S) to DNA in vivo was measured and evaluated in a quantitative manner in order to investigate whether DNA adduct formation could form a mechanistic basis for tumor induction in a carcinogenicity bioassay. [7-3H]S was administered by inhalation in a closed chamber to male and female CD rats and B6C3F1 mice. After 4.5-6 h (rats) and 6-9 h (pools of four mice), S doses of 23-39 and 85-110 mg/kg respectively had been metabolized. DNA was purified to constant specific radioactivity which was measurable in all samples. DNA was enzymatically degraded to the 3'-nucleotides which were separated by HPLC for the detection of radiolabeled nucleotide-S adducts. The fractions with the normal nucleotides contained most of the radioactivity. In mouse liver DNA, a minute but significant level of adduct radioactivity was also detected. In the units of the Covalent Binding Index CBI = (mumol adduct/mol DNA nucleotide)/(mmol chemical/kg body wt), values of 0.05-0.09 and 0.07-0.18 were calculated for males and females respectively. In the rat, no DNA adducts were detectable in the liver at a limit of detection of 0.1 CBI units. Two of the four lung samples of the female rats showed adduct-related radioactivity corresponding to 0.07 CBI units. The CBI values are compatible with styrene 7,8-oxide as the reactive intermediate. The data are compared with CBI values and carcinogenic potencies of established genotoxic carcinogens. It is concluded that the DNA-binding potency of S is so low that significant tumor induction in a standard bioassay for carcinogenicity is unlikely to be due to DNA adduct formation alone. Consequences for a human risk estimation are discussed.

Diet restriction has repeatedly been shown to reduce the incidence of spontaneous and chemically induced tumors in rodents. However, no conclusive data are available to show whether carcinogenesis by chronic exposure to a genotoxic agent can also be retarded. In this study, diet restriction to 70% was investigated for a protective effect on the formation of skin papilloma in male NMRI mice treated twice weekly with 20 nmol 7,12-dimethylbenz[a]anthracene (DMBA). Rather surprisingly, no protection was seen. Both time of onset of papilloma formation (13 weeks in both groups) and time of 50% cumulative incidence (t50; 17.5 and 18 weeks) were similar in the unrestricted and the restricted group. In contrast, a clearly protective effect was found in mice initiated with 100 nmol DMBA and promoted twice weekly with 2.5 nmol 12-O-tetradecanoylphorbol-13-acetate: the onset of papilloma formation increased from 7 to 11.5 weeks, the t50 was shifted from 8.5 to 19 weeks. Diet restriction, therefore, was not protective under conditions of chronic exposure to a genotoxic carcinogen. It cannot be considered a universal measure of cancer prevention.

Mosquitoes transmit many debilitating diseases including malaria, dengue and Zika. Odors mediate behaviors that directly impact disease transmission (blood-feeding) as well as life history events that contribute to mosquito survival and fitness (mating and oviposition, nectar foraging, larval foraging and predator avoidance). In addition to innate olfaction-mediated behaviors, mosquitoes rely on olfactory experience throughout their life to inform advantageous choices in many of these important behaviors. Previous reviews have addressed either the chemical ecology of mosquitoes, or olfactory-driven behaviors including host-feeding or oviposition. Adding to this literature, we use a holistic life history perspective to integrate and compare innate and learned olfactory behavior at various stages of mosquito development.

Background Little is known about the occurrence and epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in public transportation in the United States. This research sought to determine the background prevalence and phenotypic and genotypic characteristics of MRSA strains circulating on buses from a large, metropolitan transportation agency. Methods Electrostatic wipes were used to collect 237 surface samples from 40 buses randomly selected from July-October 2010. Six samples were collected from each bus immediately postservice and before any cleaning and disinfection. Positive isolates were analyzed for antibiotic resistance, staphylococcal cassette chromosome mec (SCCmec) type, and pulsed-field gel electrophoresis; and potential epidemiologic factors were examined. Results Of the buses, 68% (27/40) were contaminated with S aureus , and 63% (25/40) were contaminated with MRSA. Seats and seat rails were the surfaces most frequently contaminated, followed by the back door and stanchions. Most (62.9%) of the MRSA isolates were classified as community-associated MRSA clones (SCCmec type IV), and 22.9% were health care–associated MRSA clones (SCCmec type II). Of the MRSA strains, 65% (5/20) were multidrug resistant. Conclusion MRSA was frequently isolated from commonly touched surfaces in buses serving both hospital and community routes. Phenotypic and genotypic analysis demonstrated that buses may be effective mixing vessels for MRSA strains of both community and health care–associated origin.

Hair eating in nonhuman primates is thought to result from a frustrated appetitive drive produced by an inappropriate diet. To investigate whether hair eating could be reduced through changes in diet, a two-part study was conducted with a group of baboons (Papio hamadryas sp.). The first part involved changing to a twice-daily feeding routine, thus providing prolonged access to an appropriate food source. The other part involved scattering a grain mix to encourage more foraging while maintaining a once-daily feeding routine. Changing the feeding routine unexpectedly resulted in a significant increase in hair manipulation and ingestion. Providing forage did not significantly decrease hair manipulation and ingestion, but several individuals did show a reduction in these behaviors. Consuming biscuits without having to forage for them may have led to the increase in hair manipulation and consumption. Spreading grain throughout the enclosure may have failed to satisfy the urge to forage because little effort was needed to collect it. Although the current study failed to significantly decrease hair eating, it provides valuable insight into further avenues of research on the behavior.

Stereotypies that develop spontaneously in nonhuman primates can provide an effective model for repetitive stereotyped behavior in people with neurodevelopmental or obsessive-compulsive disorders. The behaviors are similar in form, are similarly affected by environmental conditions, and are improved with similar treatment methods such as enrichment, training, and drug therapy. However, because of a greater number of commonalities in these factors, nonhuman primates may serve as a better model for stereotyped behavior in individuals with autism or intellectual disability than for compulsions in individuals with obsessive-compulsive disorder. Because animal models may not be exact in all features of the disorder being studied, it is important to investigate the strengths and weaknesses of using a nonhuman primate model for stereotyped behavior in people with psychological disorders.