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Ischemia-reperfusion (IR) is a common risk factor that causes acute kidney injury (AKI). AKI is associated with dysfunction of other organs also known as distant organ injury. The liver function is often compromised in patients with AKI and in animal models. However, the underlying mechanisms are not fully understood. Inflammatory response plays an important role in IR-induced tissue injury. Although increased proinflammatory cytokines have been detected in the kidney and the distant organs after renal IR, their original sources remain uncertain. In the present study, we investigated the acute effect of renal IR on hepatic inflammatory cytokine expression and the mechanism involved. Sprague-Dawley rats that were subjected to renal IR (ischemia for 45 min followed by reperfusion for 1 h or 6 h) had increased plasma levels of creatinine, urea, and transaminases, indicating kidney and liver injuries. There was a significant increase in the expression of proinflammatory cytokine mRNA (MCP-1, TNF-α, IL-6) in the kidney and liver in rats with renal IR. This was accompanied by a significant increase in proinflammatory cytokine protein levels in the plasma, kidney, and liver. Activation of a nuclear transcription factor kappa B (NF-κB) was detected in the liver after renal IR. The inflammatory foci and an increased myeloperoxidase (MPO) activity were detected in the liver after renal IR, indicating hepatic inflammatory response and leukocyte infiltration. These results suggest that renal IR can directly activate NF-κB and induce acute production of proinflammatory cytokines in the liver. Renal IR-induced hepatic inflammatory response may contribute to impaired liver function and systemic inflammation.

The unique characteristics and healthful reputation of caffeine-free rooibos tea (RT) make it an ideal carrier for vitamin D3 supplementation, and a potential base for the addition of Saskatoon berry syrup (SBS), a natural flavor additive. The objective of this study was to determine the effect of vitamin D3 fortification and SBS addition on the flavor profile, consumer acceptability, and antioxidant properties of RT. Six formulations (RT, RT with SBS, RT with SBS and vitamin D3 , RT with vitamin D3 , green tea [GT], and GT with SBS) were evaluated by 12 trained panelists and 114 consumers. The formulations were also assessed for antioxidant capacity, physical characteristics, and untargeted phytochemical content. Sensory results revealed that the mean intensity values for berry and sweet attributes were significantly higher (P < 0.05) while bitter and astringent attributes were significantly lower when SBS was added to RT samples compared to those without syrup. Acceptability of flavor, aftertaste, and overall acceptability were also significantly higher for the RT with SBS. The addition of SBS to RT significantly increased the antioxidant capacities which may increase the related health benefits of RT. SBS contributed several polyphenols, particularly flavonoids, to the tea. Vitamin D3 added to RT formulations did not significantly affect the sensory attributes, acceptability, or antioxidant content. For the development of a functional vitamin D3 fortified iced-tea beverage that can be consumed as part of the daily diet, SBS could be a favorable flavoring additive that may provide additional health benefits.

Ischemia-reperfusion is a common cause for acute kidney injury and can lead to distant organ dysfunction. Glutathione is a major endogenous antioxidant and its depletion directly correlates to ischemia-reperfusion injury. The liver has high capacity for producing glutathione and is a key organ in modulating local and systemic redox balance. In the present study, we investigated the mechanism by which kidney ischemia-reperfusion led to glutathione depletion and oxidative stress. The left kidney of Sprague-Dawley rats was subjected to 45 min ischemia followed by 6 h reperfusion. Ischemia-reperfusion impaired kidney and liver function. This was accompanied by a decrease in glutathione levels in the liver and plasma and increased hepatic lipid peroxidation and plasma homocysteine levels. Ischemia-reperfusion caused a significant decrease in mRNA and protein levels of hepatic glutamate-cysteine ligase mediated through the inhibition of transcription factor Nrf2. Ischemia-reperfusion inhibited hepatic expression of cystathionineγ-lyase, an enzyme responsible for producing cysteine (an essential precursor for glutathione synthesis) through the transsulfuration pathway. These results suggest that inhibition of glutamate-cysteine ligase expression and downregulation of the transsulfuration pathway lead to reduced hepatic glutathione biosynthesis and elevation of plasma homocysteine levels, which, in turn, may contribute to oxidative stress and distant organ injury during renal ischemia-reperfusion.

The fairing of the launcher selected for the Space Infrared telescope for Cosmology and Astrophysics (SPICA) mission is not compatible with a primary mirror of 3.5m in diameter. Thus three alternative optical designs of the SPICA Telescope Assembly (STA) with a primary mirror of reduced size were defined and their theoretical optical performances assessed. The impact of the size reduction on the STA optical performances was then quantified. Based on the results of the study, we defined a STA optical design optimum in terms of optical performances and of accommodation of instruments in the STA focal surface.

Lingonberry grown in northern Manitoba, Canada, contains exceptionally high levels of anthocyanins and other polyphenols. Previous studies from our lab have shown that lingonberry anthocyanins can protect H9c2 cells from ischemia–reperfusion injury and anthocyanin-rich diets have been shown to be associated with decreased cardiovascular disease and mortality. Oxidative stress can impair function and trigger apoptosis in cardiomyocytes. This study investigated the protective effects of physiologically relevant doses of lingonberry extracts and pure anthocyanins against hydrogen-peroxide-induced cell death. Apoptosis and necrosis were detected in H9c2 cells after hydrogen peroxide treatment via flow cytometry using FLICA 660 caspase 3/7 combined with YO-PRO-1 and then confirmed with Hoechst staining and fluorescence microscopy. Each of the 3 major anthocyanins found in lingonberry (cyanidin-3-galactoside, cyanidin-3-glucoside, and cyanidin-3-arabinoside) was protective against hydrogen-peroxide-induced apoptosis in H9c2 cells at 10 ng·mL−1 (20 nmol·L−1) and restored the number of viable cells to match the control group. A combination of the 3 anthocyanins was also protective and a lingonberry extract tested at 3 concentrations produced a dose-dependent protective effect. Lingonberry anthocyanins protected cardiac cells from oxidative-stress-induced apoptosis and may have cardioprotective effects as a dietary modification.

“The doctor of the future will no longer treat the human frame with drugs, but will rather cure and prevent disease with nutrition”. Thomas Edison's contemplation may come to fruition if the nutritional revolution continues in its current course. Two realizations have propelled the world into a new age of personalized nutrition: (i) food can provide benefits beyond its intrinsic nutrient content, and (ii) we are not all created equal in our ability to realize to these benefits. Nutrigenomics is concerned with delineating genomic propensities to respond to various nutritional stimuli and the resulting impact on individual health. This review will examine the current technologies utilized by nutrigeneticists, the available literature regarding nutrient-gene interactions, and the translation of this new awareness into public health.

The unique characteristics and healthful reputation of caffeine-free rooibos tea (RT) make it an ideal carrier for vitamin D

Lingonberry (Vaccinium vitis-idaea L.) contains high levels of anthocyanins which are bioavailable in the kidney and may be protective against ischemia-reperfusion (IR)-induced acute kidney injury. This study investigated the effect of lingonberry juice on the IR-induced stress-activated signalling pathway and inflammatory response in the kidney.Sprague-Dawley rats subjected to kidney IR had significantly impaired kidney function, with increased activation of the JNK signalling pathway and increased inflammatory response, measured using a multiplex panel containing an extensive array of inflammatory biomarkers. In rats fed 1 mL lingonberry juice daily for 3 weeks prior to IR, kidney function was protected and attenuation of inflammatory response and JNK signalling was reflected in the reduction of the measured biomarkers. In vitro results in cultured HK-2 cells confirmed that lingonberry anthocyanins reduced JNK signalling and inflammatory gene expression after IR.This study shows, for the first time, that daily supplementation with lingonberry juice may protect against loss of kidney function induced by IR injury by modulating JNK signalling and inhibiting the subsequent inflammatory response. © 2017 Her Majesty the Queen in Right of Canada. Journal of the Science of Food and Agriculture © 2017 Society of Chemical Industry.

We present first results of a study of the submillimetre (rest frame far-infrared) properties of z~3 Lyman Break Galaxies (LBGs) and their lower-redshift counterparts BX/BM galaxies, based on Herschel-SPIRE observations of the Northern field of the Great Observatories Origins Deep Survey (GOODS-N). We use stacking analysis to determine the properties of LBGs well below the current limit of the survey. Although LBGs are not detected individually, stacking the infrared luminous LBGs (those detected with Spitzer at 24 microns yields a statistically significant submm detection with mean flux = 5.9+/-1.4 mJy confirming the power of SPIRE in detecting UV-selected high-redshift galaxies at submillimetre wavelengths. In comparison, the Spitzer 24 microns detected BX/BM galaxies appear fainter with a stacked value of = 2.7 +/-0.8 mJy. By fitting the Spectral Energy Distributions (SEDs) we derive median infrared luminosities, L_{IR}, of 2.8x10^{12} Lsun and 1.5x10^{11} Lsun for z~3 LBGs and BX/BMs, respectively. We find that $L_{IR} estimates derived from present measurements are in good agreement with those based on UV data for z~2 BX/BM galaxies, unlike the case for z~3 infrared luminous LBGs where the UV underestimates the true $L_{IR}. Although sample selection effects may influence this result we suggest that differences in physical properties (such as morphologies, dust distribution and extent of star-forming regions) between z ~3 LBGs and z~2 BX/BMs may also play a significant role.

We present spectral energy distributions (SEDs) for 68 Herschel sources detected at 5-sigma at 250, 350 and 500 mu in the HerMES SWIRE-Lockman field. We explore whether existing models for starbursts, quiescent star-forming galaxies and for AGN dust tori are able to model the full range of SEDs measured with Herschel. We find that while many galaxies (~ 56 %) are well fitted with the templates used to fit IRAS, ISO and Spitzer sources, for about half the galaxies two new templates are required: quiescent ('cirrus') models with colder (10-20 K) dust, and a young starburst model with higher optical depth than Arp 220. Predictions of submillimetre fluxes based on model fits to 4.5-24 mu data agree rather poorly with the observed fluxes, but the agreement is better for fits to 4.5-70 mu data. Herschel galaxies detected at 500 mu tend to be those with the very highest dust masses.

Evidence for the efficacy of dietary interventions in protecting against cardiovascular disease has grown significantly, with flavonoids and anthocyanins receiving special attention. Lingonberry (Vaccinium vitis-idaea L.) is a good source of these compounds, and this study examined the protective effects of wild lingonberry found in Manitoba, Canada, against ischemia-reperfusion (IR) injury. Manitoba lingonberry contained 3793 ± 27 mg gallic acid equiv, 120,501 ± 7651 μmol trolox equiv, and 575 ± 20 mg cyanidin-3-glucoside equiv per 100 g dry weight, which correspond with high total phenolic content, antioxidant activity, and anthocyanin content, respectively. A complete methanolic extract and both anthocyanin-rich and phenolic-rich fractions inhibited apoptosis in H9c2 cells during simulated IR. Lingonberry extract and fractions significantly inhibited several markers of apoptosis induced by IR, including nuclei condensation, caspase-3 activation, and MAP kinase signaling. These results provide the first analysis of Manitoba lingonberry and highlight the mechanistic importance of dietary berry compounds for cardiovascular health.

We set out to determine the ratio, q(IR), of rest-frame 8-1000um flux, S(IR), to monochromatic radio flux, S(1.4GHz), for galaxies selected at far-IR and radio wavelengths, to search for signs that the ratio evolves with redshift, luminosity or dust temperature, and to identify any far-IR-bright outliers - useful laboratories for exploring why the far-IR/radio correlation is generally so tight when the prevailing theory suggests variations are almost inevitable. We use flux-limited 250-um and 1.4-GHz samples, obtained in GOODS-N using Herschel (HerMES; PEP) and the VLA. We determine bolometric IR output using ten bands spanning 24-1250um, exploiting data from PACS and SPIRE, as well as Spitzer, SCUBA, AzTEC and MAMBO. We also explore the properties of an L(IR)-matched sample, designed to reveal evolution of q(IR) with z, spanning log L(IR) = 11-12 L(sun) and z=0-2, by stacking into the radio and far-IR images. For 1.4-GHz-selected galaxies, we see tentative evidence of a break in the flux ratio, q(IR), at L(1.4GHz) ~ 10^22.7 W/Hz, where AGN are starting to dominate the radio power density, and of weaker correlations with z and T(d). From our 250-um-selected sample we identify a small number of far-IR-bright outliers, and see trends of q(IR) with L(1.4GHz), L(IR), T(d) and z, noting that some of these are inter-related. For our L(IR)-matched sample, there is no evidence that q(IR) changes significantly as we move back into the epoch of galaxy formation: we find q(IR) goes as (1+z)^gamma, where gamma = -0.04 +/- 0.03 at z=0-2; however, discounting the least reliable data at z1., A&A Herschel Special Issue, in press as a Letter. 5 pages

Lingonberry contains more antioxidants and anthocyanins than most berries and consumption of these phytochemicals has been shown epidemiologically to reduce risk of cardiovascular disease. Since the mechanisms of protection against cellular injury require elucidation, this study examined the effects of complete lingonberry, anthocyanin-enriched, and phenolic-enriched extracts on apoptosis in H9c2 rat cardiomyoblasts during simulated ischemia-reperfusion (IR). This model induced a 20-fold increase in nuclei condensation and JNK phosphorylation and a 2-fold increase in caspase-3 activation. Despite having low antioxidant capacity, the anthocyanin-enriched extract and pure anthocyanins protected cells against apoptosis by significantly inhibiting IR-induced nuclei condensation, caspase-3 activation, and JNK phosphorylation to the same degree as the complete extract. These results indicate that anthocyanins may exert anti-apoptotic effects via mechanisms unrelated to their antioxidant capacity. This research ...

The Space Infrared Telescope for Cosmology and Astrophysi cs (SPICA) is a 3.2m cooled (below 6K) telescope mission which covers mid- and far-IR waveband with unprecedented sensitivity. An overview of recent design updates of the Scientific Instrument Assembly (SIA), composed of the telescope assembly and the instrument optical bench equipped with Focal Plane Instruments (FPIs) are presented. The FPI international science and engineering review is on-going to determine the FPI suite onboard SPICA: at present the mandatory instruments and functions to perform the unique science objectives of the SPICA mission are now consolidated. The final decision on the composition of the FPI suite is expected in early 2013. Through the activities in the current pre-project phase, several key technical issues which impact directly on the instruments’ performances and the science requirements and the observing efficiency have been identified, and extensive works are underway both at instrument and spacecraft level to reso lve these issues and to enable the confirmation of the SPICA FPI suite. Keywords: SPICA, space telescope, Joule-T homson cooler , infrared astronomy

Aims Heart disease is the leading cause of death worldwide. Ischemia–reperfusion injury can lead to apoptotic death of heart cells and subsequently heart failure. Rhodiola is an herbal medicine with two main bioactive compounds — salidroside (SAL) and tyrosol (TYR). This study aimed to investigate whether these two compounds can prevent ischemia/reperfusion-induced apoptosis in H9c2 cells. Main methods Assays for total phenolics assay and Oxygen Radical Absorbance Capacity showed high antioxidant capacity of SAL and TYR. H9c2 cells were subjected to simulated ischemia/reperfusion (IR) in the presence and absence of SAL and/or TYR, and nuclei condensation, caspase-3 activity, cytochrome c release and JNK phosphorylation were determined. Key findings In H9c2 cells, IR can lead to a 5-fold increase in p-JNK level. Apoptotic nuclei condensation, caspase-3 activity and cytochrome c release were markedly elevated, indicating the occurrence of apoptosis. SAL and TYR caused a dose-dependent inhibition of nuclear condensation. Furthermore, SAL and TYR, separately and in combination, significantly reduced caspase-3 activity, cytochrome c release and JNK activation. The anti-apoptotic effect of the combination was markedly higher than that of SAL or TYR alone. Significance The inhibition of the JNK signaling pathway is the key mechanism for the cytoprotective effect of SAL and TYR in IR-induced apoptosis.

Inflammation plays a critical role in kidney ischemia–reperfusion injury but mechanisms of increased proinflammatory cytokine expression are not completely understood. Kidney has a high expression of cystathionine‐β‐synthase (CBS) and cystathionine‐γ‐lyase (CSE) that can synthesize hydrogen sulfide. CBE and CSE are also responsible for the synthesis of cysteine, an essential precursor for glutathione, an antioxidant. Reduced hydrogen sulfide and glutathione production is associated with multiple organ injury. Although pro‐ and anti‐inflammatory effects of hydrogen sulfide have been reported, its role in ischemia–reperfusion‐induced inflammation in the kidney has not been well addressed. The aim of this study was to investigate the effect of CBS and CSE‐mediated hydrogen sulfide and glutathione production on kidney inflammatory response and the mechanism involved. The left kidney of Sprague‐Dawley rat was subjected to 45‐min ischemia followed by reperfusion for 24 h. Ischemia–reperfusion caused a significant decrease in CBS and CSE mRNA and protein levels with a concomitant reduction of glutathione and hydrogen sulfide production in the kidney while the expression of proinflammatory cytokine expression (MCP‐1, IL‐6) was elevated. Hypoxia–reoxygenation of proximal tubular cells led to a decrease in CBS and CSE expression and an increase in proinflammatory cytokine expression. Supplementation of glutathione or hydrogen sulfide donor (NaHS) effectively attenuated cytokine expression in tubular cells. These results suggested that ischemia–reperfusion impaired CBS and CSE‐mediated glutathione and hydrogen sulfide production in the kidney, which augmented the expression of proinflammatory cytokines. Regulation of CBS and CSE expression may be therapeutically relevant in alleviating ischemia–reperfusion‐induced inflammation and improving kidney function., Our study, for the first time, provides evidence that reduced CBS and CSE‐mediated hydrogen sulfide and glutathione production stimulate proinflammatory cytokine expression in the kidney upon ischemia–reperfusion injury. Restoration of CBS and CSE expression appears to be therapeutically relevant for AKI.

The Herschel Space Observatory enables us to accurately measure the bolometric output of starburst galaxies and active galactic nuclei (AGN) by directly sampling the peak of their far-infrared (IR) emission. Here we examine whether the spectral energy distribution (SED) and dust temperature of galaxies have strongly evolved since z~2.5. We use Herschel deep extragalactic surveys from 100 to 500um to compute total IR luminosities in galaxies down to the faintest levels, using PACS and SPIRE in the GOODS-North field (PEP and HerMES key programs). We show that measurements in the SPIRE bands can be used below the statistical confusion limit if information at higher spatial resolution is used to identify isolated galaxies whose flux is not boosted by bright neighbors. Below z~1.5, mid-IR extrapolations are correct for star-forming galaxies with a dispersion of only 40% (0.15dex), therefore similar to z~0 galaxies. This narrow distribution is puzzling when considering the range of physical processes that could have affected the SED of these galaxies. Extrapolations from only one of the 160um, 250um or 350um bands alone tend to overestimate the total IR luminosity. This may be explained by the lack of far-IR constraints around and above ~150um (rest-frame) on local templates. We also note that the dust temperature of luminous IR galaxies around z~1 is mildly colder by 10-15% than their local analogs and up to 20% for ULIRGs at z~1.6. Above z=1.5, distant galaxies are found to exhibit a substantially larger mid- over far-IR ratio, which could either result from stronger broad emission lines or warm dust continuum heated by a hidden AGN. Two thirds of the AGNs identified in the field with a measured redshift exhibit the same behavior as purely star-forming galaxies. Hence a large fraction of AGNs harbor star formation at very high SFR and in conditions similar to purely star-forming galaxies., Astronomy and Astrophysics, Herschel Special Issue, in press as a Letter; 5 pages

We exploit the deep and extended far infrared data sets (at 70, 100 and 160 um) of the Herschel GTO PACS Evolutionary Probe (PEP) Survey, in combination with the HERschel Multi tiered Extragalactic Survey (HerMES) data at 250, 350 and 500 um, to derive the evolution of the restframe 35 um, 60 um, 90 um, and total infrared (IR) luminosity functions (LFs) up to z~4. We detect very strong luminosity evolution for the total IR LF combined with a density evolution. In agreement with previous findings, the IR luminosity density increases steeply to z~1, then flattens between z~1 and z~3 to decrease at z greater than 3. Galaxies with different SEDs, masses and sSFRs evolve in very different ways and this large and deep statistical sample is the first one allowing us to separately study the different evolutionary behaviours of the individual IR populations contributing to the IR luminosity density. Galaxies occupying the well established SFR/stellar mass main sequence (MS) are found to dominate both the total IR LF and luminosity density at all redshifts, with the contribution from off MS sources (0.6 dex above MS) being nearly constant (~20% of the total IR luminosity density) and showing no significant signs of increase with increasing z over the whole 0.8, Comment: 32 pages, 20 figures, 9 tables. Published in MNRAS. Replaced Fig. 8 (small scaling bug in the previous version)

We study the dust properties of galaxies in the redshift range 0.10.5 with L_IR>5x10^{10} L_\odot, appears to be 2-5 K colder than that of AKARI-selected local galaxies with similar luminosities; and the dispersion in T_dust for high-z galaxies increases with L_IR due to the existence of cold galaxies that are not seen among local galaxies. We show that this large dispersion of the L_IR-T_dust relation can bridge the gap between local star-forming galaxies and high-z submillimeter galaxies (SMGs). We also find that three SMGs with very low T_dust (, Comment: 9 pages, 6 figures, To appear in MNRAS