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The Microchannel X-ray Telescope (MXT) is an innovative compact X-ray instrument on board the SVOM astronomical mission dedicated to the study of transient phenomena such as gamma-ray bursts. During 3 weeks, we have tested the MXT flight model at the Panter X-ray test facility under the nominal temperature and vacuum conditions that MXT will undergo in-flight. We collected data at series of characteristic energies probing the entire MXT energy range, from 0.28 keV up to 9 keV, for multiple source positions with the center of the point spread function (PSF) inside and outside the detector field of view (FOV). We stacked the data of the positions with the PSF outside the FOV to obtain a uniformly illuminated matrix and reduced all data sets using a dedicated pipeline. We determined the best spectral performance of MXT using an optimized data processing, especially for the energy calibration and the charge sharing effect induced by the pixel low energy thresholding. Our results demonstrate that MXT is compliant with the instrument requirement regarding the energy resolution (, 20 pages, 10 figures, accepted for publication in Experimental Astronomy

Fanon considered that colonial alienation produced multiple forms of relational pathologies, many of which expressed themselves in and through blockages in communication rather than through explicit conflict. Exposing the role of language as a vector of racial violence and colonial oppression, Fanon also presented it as a potentially revolutionary medium of decolonization. The article has two aims: firstly, to give an account of Fanon’s ongoing concern with language, in part by underscoring three very different influences in Fanon’s expansive conception of its role: Aimé Césaire, Maurice Merleau-Ponty, and François Tosquelles. Secondly, to rearticulate the function of language in Fanon’s theory of colonial disalienation by drawing on a specific trajectory in his clinico-political thought: from diagnoses of untranslatability or incommunicability to analyses of decolonization as the radical transformation of the subject’s experience of language.

Laws play an important role in emergency response capacity. During the COVID-19 outbreak, experts have noted both a lack of law where it is needed and a problematic use of laws that exist. To address those challenges, policymakers revising public health emergency laws can examine how existing laws were used during the COVID-19 response to address problems that arose during their application. Judicial opinions can provide a source of data for this review. This study used legal epidemiology methods to perform an environmental scan of global judicial opinions, published from March 1 through August 31, 2020, from 23 countries, related to government-issued COVID-19 mitigation measures. The opinions were coded, and findings categorize the measures based on: (1) the World Health Organization's May 2020 publication

Primary school practices are often bound by traditions that perpetuate compliance and skills-based, decontextualized, rote memorization activities. These histories of practice, prevalent in schools serving mostly Black and Brown children, make it inordinately difficult for students to author themselves as knowledge builders (i.e., with epistemic agency), which is a form of injustice. Engineering is a potentially fertile context to support the creation of epistemic culture, whereby young students’ assets are recognized, named, and leveraged as they create and shape the group’s disciplinary knowledge. The authors investigated this potential. The primary research question was: How do first-grade students in an urban emergent school author themselves as epistemic agents during an engineering design unit? Using a social practice theory lens and ethnographic methods, the authors studied 29 days of a materials engineering unit focusing on the teacher’s epistemic commitments, implicit meanings of knowledge in classroom discourse, and practices that opened space for students’ epistemic agency. Data collection included fieldnotes and video of class activities and teacher and student interviews. Class discussions about the properties and uses of materials yielded a rich context for studying epistemic culture. The teacher’s epistemic commitments included an eschewing of disciplinary silos, recognizing the nonlinear nature of knowledge-building about engineering, and acknowledging children’s thinking as an asset for engineering knowledge production. Examples of students’ discursive moves demonstrating epistemic agency included: reminding others about the relevance of previous lessons to the current topic, mirroring the teacher’s instructional moves, claiming voice, space, time, and material resources for knowledge-building, translating one another’s ideas, and making unsolicited connections to their lives. Young children’s intellectual assets can too easily be overlooked in traditional learning contexts. This study demonstrates the affordances of responsive engineering instruction in recognizing and building on youths’ intellectual curiosity and enthusiasm for substantively contributing to the classroom’s knowledge-generating practices.

The Space-based multi-band astronomical Variable Objects Monitor (SVOM) is a Chinese – French satellite mission due to be launched in the summer of 2022. It is composed of four instruments: ECLAIRs, for detecting X-ray and gamma-ray transients (4-250 keV); GRM, a gamma-ray spectrometer (15 keV-5 MeV); VT, a visible telescope and the Microchannel X-ray Telescope (MXT). The MXT’s main goal is to precisely localize, and spectrally characterize X-ray afterglows of Gamma-Ray Bursts. The MXT is a narrow-field-optimised lobster eye X-ray focusing telescope comprising an array of 25 square Micro Pore Optics (MPOs), with a detectorlimited field of view of ∼1 square degree, working in the energy band 0.2-10 keV. The SVOM qualification model (QM) MXT optic (MOP) was designed and built at the University of Leicester, and is the first complete, lobster eye optic to be X-ray tested. We present results from the PANTER facility (MPE), where a full calibration of the QM MOP was carried out. The response of the optic was studied at seven energies from C-K to Cu-K, and the effective area at multiple off-axis angles at each energy was measured. The focal length of the MOP was confirmed and the PSF was studied on and off-axis. In addition, we present details of the modelling and analysis, which was used to calculate the results from the test campaign. The effective area and PSF are in good agreement with the modelling, indicating that the optic is performing as expected.

Introduction Perfectionism has been related to cognitive anxiety in sports. The bidirectional association between sleep and anxiety has also been well studied. However, the relationship between sleep habits, anxiety and perfectionism in physically active young individuals hasn’t been documented yet. This was the objective of the present study. Methods 150 young, physically active participants (10 to 18 years old; 47% boys, 51% girls) completed an online survey comprised of questions extracted from the Sport Multidimensional Perfectionism Scale-2, the Competitive State Anxiety Inventory-2R and the Adolescent Sleep Habits Survey. First, independent t-tests were conducted to compare sleep habits, personal standards (PS), perceived parental pressure (PPP) and perceived coach pressure (PCP) of more anxious (ANX) to less anxious (NOANX) participants. Then, stepwise multiple linear regression analyses were computed to examine the significant sleep habits and perfectionism variables associations with competitive cognitive anxiety. Results Results show that ANX participants have higher PS (t(148)=3.19, p=0.002), less total sleep time on weekend (TST; t(148)=-2.94, p=0.004), longer sleep onset latency (SOL) on weeknights (t(128.09)=2.28, p=0.03) and report more daytime sleepiness (t(148)=3.06, p=0.003) compared to NOANX participants. The significant sleep variables and PS collectively explained 17.7% of competitive cognitive anxiety variance (p=0.00). PS was the largest predictor (β=0.27, p=0.00), followed by daytime sleepiness (β=0.21, p=0.007), weekend TST (β=-0.17, p=0.023) and weeknight SOL (β=0.16, p=.038). Conclusion These results suggest that, even though PS is associated with cognitive anxiety, sleep seem to be an important factor to consider. Specifically, increased daytime sleepiness accompanied by difficulties falling asleep during school nights and no recovery sleep during weekends significantly contribute to competitive cognitive anxiety. These results could have important implications when addressing competitive anxiety issues in young athletes. Support N/A

Introduction Children sleep is influenced by biological and socio-environmental factors. The contribution of various bedtime practices on child sleep is now well established. Emerging literature now seeks to understand the influence of more general parenting practices on child sleep. Thus, the current study examined how perceived children needs and parenting behaviors are associated with children sleep. Methods In a first study, 88 mothers (children 2-71 months) recruited during community activities completed the Child’s Sleep Habit Questionnaire, as well as a questionnaire regarding child needs (CN) and parents response to those needs (RN). Multiple linear regression analyses examined child sleep associations first to CN and then to RN, controlled by the child developmental score. Following this first study, 12 vulnerable mothers (children 39-68 months) participated in a child sleep intervention specifically developed for vulnerable families. The same measures were administered and Wilcoxon t-tests were calculated to compared pre and post intervention scores. Results Daytime sleepiness (β=.26, p=.008), sleep anxiety (β=.29, p=.007) and children cognitive development (β=-.32, p=.008) explained 31,6% of CN variance. Daytime sleepiness (β=.26, p=.03), and bedtime routine (β=-.68, p=.00), explained 20,8% of RN variance. Following the child sleep intervention, no change in CN were obtained, but a significant improvement in RN was found (Mpre=5.28 ± 1.60, Mpost=4.33 ± .65, p=.045). Conclusion Results suggest that parents perceived more needs in their child when they present higher daytime sleepiness, higher sleep anxiety and cognitive developmental difficulties. On the other hand, parents have more difficulty responding to their child needs when they see sleepiness in their child or struggle with bedtime routine. A child sleep intervention does not seem to change the perception of child needs but have a positive impact on the parent response to those needs. Support

Introduction Studies suggest that family intervention programs should consider different sources of parenting stress (PS). Knowing that child sleep has an impact on PS, this study aimed to examine if a child sleep intervention can affect PS in vulnerable families. Methods In a first study, parent and child sleep habits along with PS were assessed using Parenting Stress Index-Short Form, Pittsburgh Sleep Quality Index and homemade questions assessing child sleep. Measures were administered to 138 community mothers (children 3-79 months). Multiple linear regression analyses examined mothers and child sleep associations to PS, controlled by family income. Following results of this first study, 11 vulnerable mothers (children 39-68 months) participated in a child sleep intervention specifically developed for vulnerable families. Measures administered were the same as for study 1, but in this study, the Child Sleep Habits Questionnaire was used to assess the child sleep. Wilcoxon’s t-test compared pre and post-intervention measures. Results In the first study, sleep onset latency (SOL; β= -.25; p= .003), waketime (β= -.28; p= .003) and sleep aids (β= -.19; p= .048) explained 23.6% of PS variance. When child sleep variables were added to the model, only bedtime resistance was significant (β= -.23; p= .023) and increased the explained variance to 30.6%. Following the child sleep intervention, no significant changes in parent sleep were observed. However, results show improvement of child bedtime resistance (Mpre=10.73; Mpost=8.36; p= .027) and PS (Mpre=3.50; Mpost=3.82; p= .05). Conclusion Our results suggest that higher PS is explained by greater struggles in parents sleep (higher SOL, later waketime and higher use of sleep aids) as well as bedtime resistance behaviors in children. Furthermore, PS can be reduced by an intervention improving child bedtime resistance, even if parent sleep is not improved. Support

The SVOM (Space-based multi-band astronomical Variable Objects Monitor) French-Chinese mission is dedicated to the detection, localization and study of Gamma Ray Bursts (GRBs) and other high-energy transient phenomena. We first present the general description of the French payload composed of the ECLAIRs instrument, dedicated to GRB detection and localization and the MXT instrument, dedicated to GRB follow-up observation in soft X-ray band. Then the paper describes more in detail the design and the performances of the MXT instrument, finally a status of MXT development will be given.

Graphical abstract, The surface plasmon resonance (SPR) technique has been primarily used in the field of biology, in particular for the study of antibody-antigen interactions. Recently, polymers were introduced to form inclusion complexes. We describe here, a methodology based on surface plasmon resonance imaging to study water-resistant and reversible inclusion complexes using systems which are compatible with a cosmetic use. The purpose of this study is to follow in real time the interaction between two polymers. To carry out this study: • A biochip based on a covalent binding of one “host polymer” on a gold-activated surface was developed. • The binding of the host polymer to a guest polymer was monitored. • The presence of interactions between the β-cyclodextrins groups of the host polymer and the adamantyl functional groups of the guest polymer and the possibility of dissociating the complex were established. This technique allowed carrying out parallel assays for optimizing the amount of complexes formed, the host polymer being spotted at five concentrations. It was then possible to study the influence of the concentration in host system for two concentrations of the guest polymer. The concentration in the host polymer yielding the highest immobilization of the guest system was further determined.

The Microchannel X-ray Telescope (MXT) is a soft X-rays instrument on board SVOM, a Sino French mission. The launch is planned in 2021 by a LM-2C rocket. The main SVOM general objective is the survey of Gamma Ray Bursts, in coordination with ground telescopes. The other main on board instruments are ECLAIR (gamma, french), GRM (gamma, Chinese) and VT (visible, chinese).

This research used the SPECTRE High Performance Computing Facility at the University of Leicester.

ECLAIRs, a 2D coded-mask imaging telescope on the Sino-French SVOM space mission, will detect and locate gamma-ray bursts (GRBs) between 4 and 150 keV. The detector array is an assembly of 6400 Schottky CdTe detectors of size 4×4×1 mm3, biased from −100 V to −600 V and operated at −20 °C to minimize the leakage current and maximize the polarization time. The remarkable low-energy threshold is achieved through various steps: an extensive detectors selection, a low-noise 32 channels ASIC study, and the design of an innovative detection module called XRDPIX formed by a thick film ceramic holding 32 detectors, a high voltage grid and an HTCC substrate housing the ASIC within a hermetic cavity. In this paper, we describe the XRDPIX module and explain the results of first tests to measure the linearity and compare the sources of noise, such as leakage currents and the Equivalent Noise Charge (ENC) measured on ASIC Ceramics. We confront these values with the energy threshold and spectral resolution made with dedicated test benches. Finally, we present the superposition of 32 calibrated spectra of one XRDPIX module, showing the excellent homogeneity of the 32 detectors and the achievement of a detection threshold at 4 keV over the entire module.

The lineage relationships and fate of human dendritic cells (DCs) have significance for a number of diseases including HIV where both blood and tissue DCs may be infected. We used gene expression profiling of human monocyte and DC subpopulations sorted directly from blood and skin to define the lineage relationships. We also compared these with monocyte-derived DCs (MDDCs) and MUTZ3 Langerhans cells (LCs) to investigate their relevance as model skin DCs. Hierarchical clustering analysis showed that myeloid DCs clustered according to anatomical origin rather than putative lineage. Plasmacytoid DCs formed the most discrete cluster, but ex vivo myeloid cells formed separate clusters of cells both in blood and in skin. Separate and specific DC populations could be determined within skin, and the proportion of CD14+ dermal DCs (DDCs) was reduced and CD1a+ DDCs increased during culture, suggesting conversion to CD1a+-expressing cells in situ. This is consistent with origin of the CD1a+ DDCs from a local precursor rather than directly from circulating blood DCs or monocyte precursors. Consistent with their use as model skin DCs, the in vitro–derived MDDC and MUTZ3 LC populations grouped within the skin DC cluster. MDDCs clustered most closely to CD14+ DDCs; furthermore, common unique patterns of C-type lectin receptor expression were identified between these two cell types. MUTZ3 LCs, however, did not cluster closely with ex vivo–derived LCs. We identified differential expression of novel genes in monocyte and DC subsets including genes related to DC surface receptors (including C-type lectin receptors, TLRs, and galectins).

ECLAIRs, a 2-D coded-mask imaging camera on-board the Sino-French SVOM space missi on, will detect and locate gamma-ray bursts in near real time in the 4 - 150 keV energy band in a large field of view. The design of ECLAIRs has been driven by the objective to reach an unprecedented low-energy threshold of 4 keV. The detection plane is an assembly of 6400 Schottky CdTe detectors of size 4x4x1 mm 3 , biased from -200V to -500V and operated at -20°C. The low-energy threshold is achieved thanks to an innovative hybrid module composed of a thick film ceramic holding 32 CdTe detectors ("Detectors Ceramics"), associated to an HTCC ceramic housing a low-noise 32-channel ASIC ("ASIC Ceramics"). We manage the coupling between Detectors Ceramics and ASIC Ceramics in order to achieve the best performance and ensure the unifor mity of the detection plane. In this paper, we describe the complete hybrid XRDPIX, of which 50 flight models have been manufactured by the SAGEM company. Afterwards, we show test results obtained on Detectors Ceramics, on ASIC Ceramics and on the modules once assembled. Then, we compare and confront detectors leakage currents and ASIC ENC with the energy threshold values and FWHM measured on XRDPIX modules at the temperature of -20°C by using a calibrated radioactive source of 241Am. Finally, we study the homogeneity of the spectral properties of the 32-detector hybrid matrices and we conclude on general performance of more than 1000 detection channels which may reach the low-energy threshold of 4 keV required for the future ECLAIRs space camera.

Many viruses have developed mechanisms to evade the IFN response. Here, HIV-1 was shown to induce a distinct subset of IFN-stimulated genes (ISGs) in monocyte-derived dendritic cells (DCs), without detectable type I or II IFN. These ISGs all contained an IFN regulatory factor 1 (IRF-1) binding site in their promoters, and their expression was shown to be driven by IRF-1, indicating this subset was induced directly by viral infection by IRF-1. IRF-1 and -7 protein expression was enriched in HIV p24 antigen-positive DCs. A HIV deletion mutant with the IRF-1 binding site deleted from the long terminal repeat showed reduced growth kinetics. Early and persistent induction of IRF-1 was coupled with sequential transient up-regulation of its 2 inhibitors, IRF-8, followed by IRF-2, suggesting a mechanism for IFN inhibition. HIV-1 mutants with Vpr deleted induced IFN, showing that Vpr is inhibitory. However, HIV IFN inhibition was mediated by failure of IRF-3 activation rather than by its degradation, as in T cells. In contrast, herpes simplex virus type 2 markedly induced IFNβ and a broader range of ISGs to higher levels, supporting the hypothesis that HIV-1 specifically manipulates the induction of IFN and ISGs to enhance its noncytopathic replication in DCs.

We present the Micro-channel X-ray Telescope (MXT), a new narrow-field (about 1{\deg}) telescope that will be flying on the Sino-French SVOM mission dedicated to Gamma-Ray Burst science, scheduled for launch in 2021. MXT is based on square micro pore optics (MPOs), coupled with a low noise CCD. The optics are based on a "Lobster Eye" design, while the CCD is a focal plane detector similar to the type developed for the seven eROSITA telescopes. MXT is a compact and light (, Comment: 6 pages, 6 figures, proceedings of the conference "Swift: 10 years of Discovery", Rome, December 2-5, 2014. To be published by PoS

Epidermal Langerhans cells (eLCs) uniquely express the C-type lectin receptor langerin in addition to the HIV entry receptors CD4 and CCR5. They are among the first target cells to encounter HIV in the anogenital stratified squamous mucosa during sexual transmission. Previous reports on the mechanism of HIV transfer to T cells and the role of langerin have been contradictory. In this study, we examined HIV replication and langerin-mediated viral transfer by authentic immature eLCs and model Mutz-3 LCs. eLCs were productively infected with HIV, whereas Mutz-3 LCs were not susceptible because of a lack of CCR5 expression. Two successive phases of HIV viral transfer to T cells via cave/vesicular trafficking and de novo replication were observed with eLCs as previously described in monocyte-derived or blood dendritic cells, but only first phase transfer was observed with Mutz-3 LCs. Langerin was expressed as trimers after cross-linking on the cell surface of Mutz-3 LCs and in this form preferentially bound HIV envelope protein gp140 and whole HIV particles via the carbohydrate recognition domain (CRD). Both phases of HIV transfer from eLCs to T cells were inhibited when eLCs were pretreated with a mAb to langerin CRD or when HIV was pretreated with a soluble langerin trimeric extracellular domain or by a CRD homolog. However, the langerin homolog did not inhibit direct HIV infection of T cells. These two novel soluble langerin inhibitors could be developed to prevent HIV uptake, infection, and subsequent transfer to T cells during early stages of infection.

Ohmic CdZnTe and CdTe detectors have been successfully used in high-energy missions such as IBIS on-board INTEGRAL and the Swift-BAT in the past two decades. Such detectors provide very good quantum efficiency in the hard X-ray band. For the future generation of hard X-ray coded mask detectors, a higher sensitivity will be required. A way to achieve this is to increase the effective area of the pixilated detection plane, to change the mask pattern and/or the properties of the semi-conductors paving the detection plane. For the future Chinese-French Gamma-ray burst mission SVOM, the GRB trigger camera ECLAIRs will make use of a new type of high-energy detectors, the Schottky CdTe detectors. Such detectors, when reversely biased, are known to present very low leakage current, resulting in lower values of the low-energy threshold (down to 4 keV or less) than for previous missions (i.e. > 10 keV for the Swift-BAT and INTEGRAL/IBIS). Such low values will enable ECLAIRs with a moderate geometrical area of 1024 cm2 and a low-energy threshold of 4 keV to be more sensitive to high-redshift GRBs (emitting mainly in X-rays) than the Swift-BAT with a higher effective area and low-energy threshold. However, the spectral performance of such detectors are known to degrade over time, once polarized, due to the polarization effect that strongly depends on the temperature and the bias voltage applied to the detectors. In this paper, we present an intensive study of the properties of Schottky CdTe detectors as used on SVOM/ECLAIRs such as I-V characteristics, polarization effect, activation energy and low temperature annealing effects. We discuss the implications of these measurements on the use of this type of detectors in future high-energy instruments.

ECLAIRs, a 2-D coded-mask imaging camera on-board the Sino-French SVOM space mission, will detect and locate Gamma-ray bursts (GRBs) in near real time in the 4-150 keV energy band. The design of ECLAIRs has been mainly driven by the objective of achieving a low-energy threshold of 4 keV, unprecedented for this type of instrument. The detection plane is an assembly of 6400 Schottky CdTe semiconductor detectors of size 4x4x1 mm 3 organized on elementary hybrid matrices of 4x8 detectors. The detectors will be polarized from -300V to -500V and operated at -20°C to reduce both the leakage current and the polarization effect induced by the Schottky contact. The remarkable low-energy threshold homogeneity required for the detection plane has been achieved thanks to: i) an extensive characterization and selection of the detectors, ii) the development of a specific low-noise 32-channel ASIC, iii) the realization of an innovative hybrid module composed of a thick film ceramic (holding 32 CdTe detectors with their high voltage grid), associated to an HTCC ceramic (housing the ASIC chip within an hermetic enclosure). In this paper, we start describing a complete hybrid matrix, and then the manufacturing of a first set of 50 matrices (representing 1600 detectors, i.e. a quarter of ECLAIRs detector’s array). We show how this manufacturing allowed to validate the different technologies used for this hybridization, as well as the industrialization processes. During this phase, we systematically measured the leakage current on Detector Ceramics after an outgassing, and the Equivalent Noise Charge (ENC) for each of the 32 channels on ASIC Ceramics, in order to optimize the coupling of the two ceramics. Finally, we performed on each hybrid module, spectral measurements at -20°C in our vacuum chamber, using several calibrated radioactive sources ( 241 Am and 55 Fe), to check the performance homogeneity of the 50 modules. The results demonstrated that the 32-detector hybrid matrices presented homogeneous spectral properties and that a lowenergy threshold of 4 keV for each detector could be reached. In conclusion, our hybrid module has obtained the performance required at the SVOM mission level and successfully withstood the space environment tests (TRL 6/7). This development phase has given us the opportunity to build a detector’s array prototype (Engineering Model) equipped with 50 hybrid modules. Thanks to this prototype we are in the process of validating a complete detection chain (from the detectors to the backend electronics) and checking the performance. In addition it enables us to consolidate the instrument’s mechanical and thermal design, and to write preliminary versions of the quality procedures required for integration, functional tests and calibration steps. At the end of this prototype development and testing, we will be ready to start the detailed design of the detection plane Flight Model.

The Microchannel X-Ray Telescope will be implemented on board the SVOM space mission to observe the afterglow of gamma-ray bursts and localize them with 2 arcmin precision. The optical system is based on microchannel plates assembling in Wolter-I configuration to focus the X-rays in the focal plane, like done for the MIXS telescope of the BepiColombo ESA mission. The sensor part is a 256 × 256 pixel pnCCD from the Max-Planck Institute for Extraterrestrial Physics for high resolution spectroscopy and high quantum efficiency over 0.2 – 10 keV energy range, based on the same technology and design as the eROSITA telescopes for the Russian-German SRG mission. CEA-Irfu (Saclay) is in charge of the design and the realization of the camera, including the focal plane, the calibration wheel, the front-end electronics, the structure housing for background shielding and the active cooling system. A prototype of the full detection chain and the acquisition system was set up. The paper presents the preliminary design of the electrical, mechanical and thermal architectures of the camera. It focuses on the fabrication and testing of the critical elements of the design and concludes on the on-going developments.

The SVOM (Space-based multi-band astronomical Variable Objects Monitor) French-Chinese mission is dedicated to the detection, localization and study of Gamma Ray Bursts (GRBs) and other high-energy transient phenomena. We first present the major principles of the SVOM system including the alert system providing near-real-time GRB localizations to large ground-based telescopes. Then the paper describes the definition of the SVOM payload and more particularly the French payload composed of the ECLAIRs instrument, dedicated to GRB detection and positioning, and the MXT instrument, dedicated to GRB followup observation in soft X-ray band.

We present ECLAIRs, the Gamma-ray burst (GRB) trigger camera to fly on-board the Chinese-French mission SVOM. ECLAIRs is a wide-field ($\sim 2$\,sr) coded mask camera with a mask transparency of 40\% and a 1024 $\mathrm{cm}^2$ detection plane coupled to a data processing unit, so-called UGTS, which is in charge of locating GRBs in near real time thanks to image and rate triggers. We present the instrument science requirements and how the design of ECLAIRs has been optimized to increase its sensitivity to high-redshift GRBs and low-luminosity GRBs in the local Universe, by having a low-energy threshold of 4 keV. The total spectral coverage ranges from 4 to 150 keV. ECLAIRs is expected to detect $\sim 200$ GRBs of all types during the nominal 3 year mission lifetime. To reach a 4 keV low-energy threshold, the ECLAIRs detection plane is paved with 6400 $4\times 4~\mathrm{mm}^2$ and 1 mm-thick Schottky CdTe detectors. The detectors are grouped by 32, in 8x4 matrices read by a low-noise ASIC, forming elementary modules called XRDPIX. In this paper, we also present our current efforts to investigate the performance of these modules with their front-end electronics when illuminated by charged particles and/or photons using radioactive sources. All measurements are made in different instrument configurations in vacuum and with a nominal in-flight detector temperature of $-20^\circ$C. This work will enable us to choose the in-flight configuration that will make the best compromise between the science performance and the in-flight operability of ECLAIRs. We will show some highlights of this work., 15 pages, 12 figures, Proceeding SPIE - Montreal 2014

We present the Microchannel X-ray Telescope, a new light and compact focussing telescope that will be flying on the Sino-French SVOM mission dedicated to Gamma-Ray Burst science. The MXT design is based on the coupling of square pore micro-channel plates with a low noise pnCCD. MXT will provide an effective area of about 50 cmsq, and its point spread function is expected to be better than 3.7 arc min (FWHM) on axis. The estimated sensitivity is adequate to detect all the afterglows of the SVOM GRBs, and to localize them to better then 60 arc sec after five minutes of observation., Comment: 12 pages, 8 figures, to be published in SPIE Astronomical Telescopes + Instrumentation, Montreal, June 2014

In order to demonstrate the feasibility of a compact telescope with both wide field of view and high resolution, a demonstration model has been built and tested at CNES. This 1.08-m focal length, f/6 three-mirror anastigmat telescope has excellent image quality over its whole 8.4 ° × 1.4 ° field of view: modulation transfer function is about 0.54 @ 77 1p.mm −1 (detector Nyquist frequency). The focal plane comprises two channels, one panchromatic with 24 000 pixels and one multispectral with 12 000 pixels in each of its 3 spectral bands. The whole telescope (mirrors, structure, baffles and focal plane) weighs less than 45 kg. The conception has been done in order to favor structure simplicity and short alignment time while retaining high stability and good image quality. The first part of this paper will focus on the instrument design, and the second part on the manufacturing and test results.

HIV-1 is taken up by immature monocyte derived dendritic cells (iMDDCs) into tetraspanin rich caves from which the virus can either be transferred to T lymphocytes or enter into endosomes resulting in degradation. HIV-1 binding and fusion with the DC membrane results in low level de novo infection that can also be transferred to T lymphocytes at a later stage. We have previously reported that HIV-1 can induce partial maturation of iMDDCs at both stages of trafficking. Here we show that CD45+ microvesicles (MV) which contaminate purified HIV-1 inocula due to similar size and density, affect DC maturation, de novo HIV-1 infection and transfer to T lymphocytes. Comparing iMDDCs infected with CD45-depleted HIV-1BaL or matched non-depleted preparations, the presence of CD45+ MVs was shown to enhance DC maturation and ICAM-1 (CD54) expression, which is involved in DC∶T lymphocyte interactions, while restricting HIV-1 infection of MDDCs. Furthermore, in the DC culture HIV-1 infected (p24+) MDDCs were more mature than bystander cells. Depletion of MVs from the HIV-1 inoculum markedly inhibited DC∶T lymphocyte clustering and the induction of alloproliferation as well as limiting HIV-1 transfer from DCs to T lymphocytes. The effects of MV depletion on these functions were reversed by the re-addition of purified MVs from activated but not non-activated SUPT1.CCR5-CL.30 or primary T cells. Analysis of the protein complement of these MVs and of these HIV-1 inocula before and after MV depletion showed that Heat Shock Proteins (HSPs) and nef were the likely DC maturation candidates. Recombinant HSP90α and β and nef all induced DC maturation and ICAM-1 expression, greater when combined. These results suggest that MVs contaminating HIV-1 released from infected T lymphocytes may be biologically important, especially in enhancing T cell activation, during uptake by DCs in vitro and in vivo, particularly as MVs have been detected in the circulation of HIV-1 infected subjects., Author Summary Dendritic cells (DCs) are vital for immune recognition of pathogens as they capture, internalise, degrade and present foreign peptides to T lymphocytes. It is thought that HIV-1 hijacks the DCs functions, such as migration and maturation, to increase contact with the major target cell CD4+ T lymphocytes leading to dissemination throughout the body. Currently there is still some controversy over the ability of HIV-1 to infect and mature DCs, which may be due to differences in the inoculum used. Here we examined the effect of contaminating microvesicles (MVs) identified in HIV-1 preparations on HIV-1 modulation of DC function. We show that when MVs are present with HIV-1, the inoculum induces greater DC maturation and adhesion probably via cellular HSP90α and β and viral nef within the MVs. The functional consequences are reduced de novo replication of HIV-1 but increased clustering with T lymphocytes, resulting in increased T lymphocyte alloproliferation and HIV-1 transfer. As MVs are produced in HIV-1 susceptible cells and would be present in vivo due to HIV-1 induced cell death and hence are physiologically relevant, these results also indicate that MVs present in HIV-1 inocula should be considered when assessing HIV∶DC interactions.

Background: Efavirenz (EFV) causes neuropsychiatric side-effects and an unfavorable blood lipid profile. We investigated the effect of replacing EFV with raltegravir (RAL) on patient preference, daytime sleepiness, sleep quality, anxiety, and lipid levels. Method: Switch-ER was a randomized, double-blind, cross-over study. Patients who tolerated EFV, with less than 50copies/ml HIV-RNA, were randomized into two groups: the RAL-first group started with RAL (400mg twice daily) and EFV placebo, and the EFVfirst group with EFV (600mg once daily) and RAL placebo. After 2 weeks, both groups switched to the alternate regimen. The primary endpoint was patient preference for the first or the second regimen, assessed after 4 weeks. Results: Fifty seven participants were enrolled with a median CD4 cell count 600/ml, and duration of previous EFV therapy 3.4 years. Fifty three participants completed the study. When asked about treatment preference after 4 weeks, 22 preferred RAL and 12 preferred EFV, whereas 19 did not express a preference. A significant difference in anxiety and stress scores favoring RAL (P ¼0.04 and 0.03, respectively) was observed. Median plasma cholesterol levels decreased by 0.4mmol/l (16mg/dl, P

The Spectral and Photometric Imaging Receiver (SPIRE), is the Herschel Space Observatory`s submillimetre camera and spectrometer. It contains a three-band imaging photometer operating at 250, 350 and 500 microns, and an imaging Fourier Transform Spectrometer (FTS) which covers simultaneously its whole operating range of 194-671 microns (447-1550 GHz). The SPIRE detectors are arrays of feedhorn-coupled bolometers cooled to 0.3 K. The photometer has a field of view of 4' x 8', observed simultaneously in the three spectral bands. Its main operating mode is scan-mapping, whereby the field of view is scanned across the sky to achieve full spatial sampling and to cover large areas if desired. The spectrometer has an approximately circular field of view with a diameter of 2.6'. The spectral resolution can be adjusted between 1.2 and 25 GHz by changing the stroke length of the FTS scan mirror. Its main operating mode involves a fixed telescope pointing with multiple scans of the FTS mirror to acquire spectral data. For extended source measurements, multiple position offsets are implemented by means of an internal beam steering mirror to achieve the desired spatial sampling and by rastering of the telescope pointing to map areas larger than the field of view. The SPIRE instrument consists of a cold focal plane unit located inside the Herschel cryostat and warm electronics units, located on the spacecraft Service Module, for instrument control and data handling. Science data are transmitted to Earth with no on-board data compression, and processed by automatic pipelines to produce calibrated science products. The in-flight performance of the instrument matches or exceeds predictions based on pre-launch testing and modelling: the photometer sensitivity is comparable to or slightly better than estimated pre-launch, and the spectrometer sensitivity is also better by a factor of 1.5-2., Comment: Accepted for publication in Astronomy & Astrophyics (Herschel first results special issue)

Dendritic cells (DCs) play a key role in the pathogenesis of HIV infection. HIV interacts with these cells through 2 pathways in 2 temporal phases, initially via endocytosis and then via de novo replication. Here the transcriptional response of human DCs to HIV-1 was studied in these phases and at different stages of the virus replication cycle using purified HIV-1 envelope proteins, and inactivated and viable HIV-1. No differential gene expression was detected in response to envelope. However, more than 100 genes were differentially expressed in response to entry of viable and inactivated HIV-1 in the first phase. A completely different set of genes was differentially expressed in the second phase, predominantly in response to viable HIV-1, including up-regulation of immune regulation genes, whereas genes encoding lysosomal enzymes were down-regulated. Cathepsins B, C, S, and Z RNA and protein decreased, whereas cathepsin L was increased, probably reflecting a concomitant decrease in cystatin C. The net effect was markedly diminished cathepsin activity likely to result in enhanced HIV-1 survival and transfer to contacting T lymphocytes but decreased HIV-1 antigen processing and presentation to these T cells.

The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.

Radiation therapy accelerators require highly accurate dose deposition and the output must be monitored frequently and regularly. Ionization chambers are the primary tool for this control, but their size and fragility make them unsuitable for use during patient treatment. In collaboration with a French center for cancer treatment in Caen, we describe the development and testing of a low-cost radiotherapy dosimeter (SDM) based on scintillating fibers and signal processing to reduce the effect of Cerenkov radiation background. The employment of photodiodes for light collection reduces the cost relative to systems using photomultipliers (PMTs). However, we have also developed a highly sensitive system that uses PMTs for very low-dose deposition for Brachytherapy. Comparison with standard ionization chambers shows about a 1% difference over a range of 6- to 25-MV photons. This dosimeter is 1 mm in diameter and can be placed where ever required including inside the body. Its small size and flexibility make it useful for delimiting critical regions, where organs may be very radiation sensitive.

With the 3S program (the French acronym for SPOT System Follow-on), CNES intends to continue the SPOT Earth Observation mission with the purpose of achieving severe costs reduction. This increases the need for new, lighter, more compact technologies for the payload. Therefore, CNES has launched preliminary studies in some critical payload domains. The paper deals with the work done in order to demonstrate the feasibility of a small telescope that can be used onboard a mini-satellite. A Three-Mirror Anastigmat (TMA) telescope breadboard has been manufactured using the same technologies that would be required to provide in-orbit stability. The TMA has excellent image quality over its whole 8.4 X 1.4 degree FOV and an intrinsic compactness that makes it much smaller than its focal length: as a result, the whole telescope weighs about 40 kg. The paper particularly focuses on the telescope design, the alignment method and the optical performance under stable laboratory environment. The behavior of the telescope under space environment is described, as well as the tests conducted to validate the computed optical performance under thermal variations and vibrations conditions.© (1999) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.

Excerpt Interest in the metabolic events associated with dithiazanine iodide intoxication was stimulated by the observation of lactic acidosis in a patient who absorbed dithiazanine from the gastro...

Lipid hemodialysis was successful in an elderly patient who had accidentally ingested camphorated oil.