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Acetabularia jalakanyakae Sp. Nov. (Fig. 1) Description The main body is comprised into three regions: a basal part with a rhizoidal holdfast, a middle region with a long stalk, and the topmost upper part with an umbrella-shaped circular cap. It consists of an unbranched cylindrical stalk. Stalk length is 20 ��� 40 mm with a cap diameter of 5 ��� 13 mm. The cap is completely fused, having 40 ��� 60 cap rays. The outer end of each ray forms a pointed structure. The outer ring (Corona inferior) consists of slender lobes with bifurcation. The inner ring (Corona superior) has 40 ��� 60 lobes without bifurcations. The inner ring comprised of 6 or 7 hairs per lobe and shows protrusions inside. The developing cap shows a swollen lobe inside and early partition at inner and outer rings. Holotype Collected from intertidal rocks at Port Blair, Andaman and Nicobar Islands, India. Herbarium voucher is deposited at Agharkar Research Institute, Pune, India, (Index Herbarium code: AHMA), under voucher no. AHMA - 32437. The DNA sequence of 18S rDNA partial region of the isolate generated and deposited at GenBank under accession # MT371394. Isotype Isotype deposited at the Central University of Punjab, Bathinda (Index Herbarium code: CUPB), under voucher no. CUPB-ACT-2019-1. Etymology Specific epithet which is feminine noun in genitive means ���mermaid��� in Sanskrit to refer the aesthesis of the cap that resembles mermaid���s umbrella. Limitations The study analysed only a few algal samples from one site in Andaman Islands. More efforts to study the species biogeography would have added value to this report. The study did not analyse the life cycle patterns as the investigation did not involve any algal culturing. As life cycle and ontogeny are highly informative, this would have significantly improved the manuscript. Additionally, the length of generated sequence is only 234 bp and all four mismatches with the closest hit (A. dentata) were all ambiguities (N or R). However, strength of morphological synapomorphies stands valid for the description of new species., Published as part of Saini, K C, Madhu, A, Kohli, R K, Gupta, K & Bast, F, 2021, Morpho-molecular assessment of Acetabularia jalakanyakae Sp. Nov. (Dasycladales, Chlorophyta) - a new species from Andaman and Nicobar Islands, India, pp. 701-708 in Indian Journal of Geo Marine Sciences 50 (9) on pages 706-707, DOI: 10.5281/zenodo.6344082

Saini, K C, Madhu, A, Kohli, R K, Gupta, K, Bast, F (2021): Morpho-molecular assessment of Acetabularia jalakanyakae Sp. Nov. (Dasycladales, Chlorophyta) - a new species from Andaman and Nicobar Islands, India. Indian Journal of Geo Marine Sciences 50 (9): 701-708, DOI: http://doi.org/10.5281/zenodo.6344082, {"references":["1 Berger S, Fettweiss U, Gleissberg S, Liddle L B, Richter U, et al., 18s rDNA phylogeny and evolution of cap development in Polyphysaceae (formerly Acetabulariaceae; Dasycladales, Chlorophyta), Phycologia, 42 (5) (2003) 506- 561. https://doi.org/10.2216/i0031-8884-42-5-506.1","2 Hammerling J, Nucleo-cytoplasmic relationships in the development of Acetabularia, Int Rev Cytol, 2 (1953) 475- 498. https://doi.org/10.1016/S0074-7696(08)61042-6","3 Zechman F W, Phylogeny of the Dasycladales (Chlorophyta, Ulvophyceae) based on analyses of rubisco large subunit (rbcL) gene sequences 1, J Phycol, 39 (4) (2003) 819-827.","4 Gulbrandsen O S, Andresen I J, Krabberod A K, Brate J & Shalchian-Tabrizi K, Phylogenomic analysis restructures the Ulvophyceae, J Phycol, 57 (4) (2021) 1223-1233. https://doi.org/10.1111/jpy.13168","5 Henry I M, Wilkinson M D, Hernandez J M, Schwarz-Sommer Z, Grotewold E, et al., Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum, BMC Plant Biol, 4 (3) (2004) pp. 15. https://doi.org/10.1186/1471-2229-4-3","6 Berger S & Liddle L B, The life cycle of Acetabularia (Dasycladales, Chlorophyta): Textbook accounts are wrong, Phycologia, 42 (2) (2003) 204-207. https://doi.org/10.2216/ i0031-8884-42-2-204.1","7 Palmer J D, Comparative organization of chloroplast genomes, Annu Rev Genet, 19 (1) (1985) 325354. DOI: 10.1146/ annurev.ge.19.120185.001545","8 Green B R, Protein synthesis by isolated Acetabularia chloroplasts. In vitro synthesis of the apoprotein of the p-700-chlorophyll a-protein complex (cp i), Biochim Biophys Acta Nucleic Acids Protein Synth, 609 (1) (1980) 107-120. https://doi.org/10.1016/0005-2787(80)90205-1","9 Minowa K & Shihira-Ishikawa I, Cap-morphogenesis in a giant unicellular alga, Acetabularia caliculus, Plant Morphol, 5 (2) (1993) 83-92. https://doi.org/10.5685/ plmorphol.5.83","10 Sawitzky H, Gleissberg S & Berger S, Phylogenetic implications of patterns of cap development in selected species of Acetabularia polyphysa (Dasycladales, Chlorophyta), Phycologia, 37 (6) (1998) 478-485. https://doi.org/10.2216/ i0031-8884-37-6-478.1","11 Dumais J & Harrison L G, Whorl morphogenesis in the Dasycladalean algae: The pattern formation viewpoint, Philos Trans R Soc Lond B Biol Sci, 355 (1394) (2000) 281- 305. DOI: 10.1098/rstb.2000.0565","12 Arora M & Sahoo D, Growth forms and life histories in green algae, In: The Algae World, edited by D Sahoo & J Seckbach, (Springer Ltd, New York), 2015, pp. 121-175. DOI 10.1007/978-94-017-7321-8_5","13 Graf zu Solms-Laubachin H, Monograph of the Acetabularieae, Trans Linn Soc Lond, 2nd Ser: Botany, 5 (1) (1895) 1-39.","14 Valet G, Contribution a l'etude des Dasycladales, 1. Morphogenese, Nova Hedwigia, 16 (1968) 21-82.","15 Olsen J L, Stam W T, Berger S & Menzel D, 18s rDNA and evolution in the Dasycladales (Chlorophyta): Modern living fossils 1, J Phycol, 30 (4) (1994) 729-744. https://doi.org/ 10.1111/j.0022-3646.1994.00729.x","16 Barattolo F, Ionesi V & Tibuleac P, Anew Polyphysacean alga from the Miocene of Romania and its biomineralization, Acta Palaeontol Pol, 64 (1) (2019) 85-100. doi: https://doi.org/ 10.4202/app.00537.2018","17 Guiry M D & Guiry G M (eds.), AlgaeBase, world-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org version (05/2021).","18 Palanisamy M, Seaweeds of South Andaman: Chidiyatapu, North Bay and Viper Island, Proceedings of the International Day for Biological Diversity, Marine Biodiversity, Uttar Pradesh State Biodiversity Board, 22nd May 2012, pp. 49-58.","19 Karthick P, Mohanraju R, Ramesh C, Murthy K N & Narayana S, Distribution and diversity of seaweeds in north and south Andaman Island, Seaweed Res Utilin, 35 (2013) 8-16.","20 Silva P C, Basson P W & Moe R L, Catalogue of the benthic marine algae of the Indian ocean, Univ of California Press, 79 (1996) 890-893.","21 White T J, Bruns T, Lee S & Taylor J, Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, PCR Protocols: A Guide to Methods and Applications, 18 (1) (1990) 315-322. DOI: 10.1016/b978-0- 12-372180-8.50042-1.","22 Bast F, Bhushan S & John A A, DNA barcoding of a new record of epi-endophytic green algae Ulvella leptochaete (Ulvellaceae, Chlorophyta) in India, J Biosci, 39 (4) (2014) 711-716. DOI: 10.1007/s12038-014-9459-3","23 Holm-Hansen C & Vainio K, Sequencing of viral genes, In: Molecular Epidemiology of Microorganisms, edited by D Caugant, (Humana Press, Totowa, NJ), 2009, pp. 203-215. DOI: 10.1007/978-1-60327-999-4_16","24 Edgar R C, Muscle: Multiple sequence alignment with high accuracy and high throughput, Nucleic Acids Res, 32 (5) (2004) 1792-1797. https://doi.org/10.1093/ nar/gkh340","25 Kumar S, Stecher G, Li M, Knyaz C & Tamura K, Mega X: Molecular evolutionary genetics analysis across computing platforms, Mol Biol Evol, 35 (6) (2018) 1547-1549. doi: 10.1093/molbev/msy096","26 Raftery A E, Bayes factors and BIC: Comment on -A critique of the Bayesian Information Criterion for model selection‖, Sociol Methods Res, 27 (3) (1999) 411-427. DOI: 10.1177/0049124199027003005","27 Strimmer K & Von Haeseler A, Quartet puzzling: Aquartet maximum-likelihood method for reconstructing tree topologies, Mol Biol Evol, 13 (7) (1996) 964-969. DOI: 10.1093/oxfordjournals.molbev.a025664","28 Bast F, Sequence similarity search, multiple sequence alignment, model selection, distance matrix and phylogeny reconstruction, Nat Protoc Exchange, (2013). DOI: 10.1038/ protex.2013.065","29 Hennig W, Phylogenetic systematics, Annu Rev Entomol, 10 (1) (1965) 97-116. https://doi.org/10.1146/annurev.en. 10.010165.000525"]}