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In the present study fibroblasts seeded matrices prepared from bovine pericardium was evaluated in the repair of full thickness skin defects in rats. The pericardium tissue from buffalo was treated with 1% sodium dodecyl sulphate (SDS) for 48 hours showed complete acellularity with normal thickness and arrangements of collagen fibres. In vitro primary mouse embryonic fibroblasts (p-MEF) cells were cultured and seeded on the acellular pericardium matrix (APM) using static seeding technique. Forty eight (48) rats of either sex were randomly divided into 4 groups of 12 animals each. Under general anaesthesia a 20x20 mm2 full thickness skin defect was created on dorsal thoracic area. The defect in group A (control) was repaired with standard dressing material whereas, groups B, C and D animals were repaired with autograft, APM and p-MEF seeded APM, respectively. Wound healing was evaluated on the basis of clinical, gross, immunological and histopathological observations. On the basis of different observations recorded it can be said that APM have wound healing potency however, seeding with p-MEF increases the wound healing potency. APM seeded p-MEF was found to be a novel biomaterial which has amphipathic character in full thickness skin repair and enhances wound healing. [ABSTRACT FROM AUTHOR]

Skin defects often arise following acute trauma, chronic wounds and burns. Although, various materials have been described to restore the integrity of full thickness skin defects, no material has yet proved to be optimal. Hence, in this study primary mouse embryonic fibroblasts (p-MEF) were cultured and seeded on bubaline acellular diaphragm matrix (ADM) to evaluate its healing potential on full thickness skin wounds. A 20 x 20 mm2 sized full thickness skin defect was created on dorsum of forty eight Sprague Dawley rats and randomly divided into four equal groups. The defect in group I (control) was left open whereas, groups II, III and IV were covered with autograft, ADM and p-MEF seeded ADM, respectively. Wound healing was evaluated on the basis of gross, immunologic and histopathologic observations. Gross healing observation with p-MEF seeded ADM group showed significantly (P < 0.05) higher wound contraction as compared to others. There was higher B-cell response in animals with ADM and p-MEF seeded ADM implants. Stimulation index of spleenocytes was higher with p-MEF seeded ADM as compared to ADM by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay. The p-MEF seeded ADM implantation caused markedly fast wound closure with well-formed granulation tissue dominated by fibroblast proliferation, collagen deposition, and complete early regenerated epithelial layer. On critical analysis of the observed parameters it was found that p-MEF seeded ADM has healing potential and shown better healing of full thickness skin wounds in rats. [ABSTRACT FROM AUTHOR]

This paper describes the extraction techniques for decellularising the rat dermis. The rat skin was procured after euthanizing rats. The rat skin was then first processed to remove the epithelium (de-epithelialization) by subjecting it to hypertonic solution for different time intervals. Later the de-epithelialized tissues were subjected to five different protocols which includes hypertonic solution, TritonX-100, sodium dodecyl sulphate and sodium deoxycholate (1% and 2%) for different time intervals for decellularizing the tissues. Tissue samples were collected at different time intervals for histological examinations. In hypertonic solution for 8 hours resulted in complete de-epithelialization of rat dermis. At 48 hours, all the samples treated with different protocols showed complete acellularity with removal of cellular debris from tissues. [ABSTRACT FROM AUTHOR]

The rat skin was de-epithelialized using hypertonic saline solution and further decellularized with 1% sodium deoxycholate. The primary mouse embryonic fibroblast (P-MEF) cells were seeded on acellular dermal matrix (ADM). Fortyfive rats were randomly divided into 3 groups of 15 animals each. A 20×20mm2 full thickness skin defect was created on dorsum. The defect in group-I was dressed with standard dressing material, group-II with ADM, and group-III ADM seeded with P-MEF. In group- III, complete healing was observed at day21. The values of hydroxyproline contents in group III were significantly higher on days14 and 21 as compared to group-I. Significant decrease in glucosamine content was observed up to day28 in group-III. Native graft antigen challenged animals showed highest B-cell response. The acellular graft antigen challenged animals showed no B-cell response. Biopsy specimens showed minimum histopathological scores in group-III. The level of MDA and SOD increased significantly up to day14 in group-I. On day3, significantly lower values of GSH were observed in group-I whereas, significantly higher values of catalase were observed in all groups. SEM examination revealed well spread fibroblasts over the scaffold in group-III. These results indicate that bioengineered grafts can be used clinically to manage full thickness skin wounds. [ABSTRACT FROM AUTHOR]