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Introduction: While learning laparoscopic procedures, the physician must face the difficulties specific to this method. They include two-dimensional vision, disturbed eye-hand coordination, and lack of touch sense control. In this study, we examined the impact of different types of training on the performance of laparoscopic procedures on box trainers. We took into account the individual manual skills of the participants. Aim: This study aimed to assess the effectiveness of a self-built laparoscopic trainer and the influence of arcade smartphone games on improving laparoscopic psychomotor skills. Material and methods: Students with no experience in laparoscopy participated in the study. They took part in the Minnesota Manual Dexterity Test (MMDT). Then, they were divided randomly into three groups. The 1st group had a 3-hour laparoscopic training. The 2nd group was performing visual-manual tasks using a phone application. The 3rd group took part in the final test without prior preparation. The final test included transferring sponges using the laparoscopic trainer and cutting a latex glove. Results: There was a statistically significant difference between the groups in the results of the sponge-transferring test and the glove-cutting speed. There was no correlation between perforations during the cutting of latex gloves and the cutting length. Conclusions: The laparoscopic trainer – also self-built – is a valuable tool for laparoscopic training. The use of arcade smartphone games contributes to increased laparoscopic psychomotor skills. [ABSTRACT FROM AUTHOR] more...

Hemolymph enables communication between organs in insects and ensures necessary coordination and homeostasis. Its composition can provide important information about the physiological state of an insect and can have diagnostic significance, which might be particularly important in the case of harmful insects subjected to biological control. Galleria mellonella Linnaeus 1758 (Lepidoptera: Pyralidae) is a global pest to honey bee colonies. The hemolymph of its larvae was examined after infection with the soil fungus Conidiobolus coronatus (Constantin) Batko 1964 (Entomophthorales). It was found that after one hour of contact with the fungus, the volume of the hemolymph increased while its total protein content decreased. In larvae with a high pathogen load, just before death, hemolymph volume decreased to nearly initial levels, while total protein content and synthesis (incorporation of 35S-labeled methionine) increased. The hemolymph polypeptide profile (SDS-PAGE followed by autoradiography) of infected insects was significantly different from that of healthy larvae. Hemocytes of infected larvae did not surround the fungal hyphae, although they encapsulated small foreign bodies (phase contrast microscopy). Infection had a negative effect on hemocytes, causing oenocyte and spherulocyte deformation, granulocyte degranulation, plasmatocyte vacuolization, and hemocyte disintegration. GC-MS analysis revealed the presence of 21 compounds in the hemolymph of control insects. C. coronatus infection caused the appearance of 5 fatty acids absent in healthy larvae (heptanoic, decanoic, adipic, suberic, tridecanoic), the disappearance of 4 compounds (monopalmitoylglycerol, monooleoylglycerol, monostearin, and cholesterol), and changes in the concentrations of 8 compounds. It remains an open question whether substances appearing in the hemolymph of infected insects are a product of the fungus or if they are released from the insect tissues damaged by the growing hyphae. [ABSTRACT FROM AUTHOR] more...

Benzyl alcohol (E1519) is an aromatic alcohol used in the pharmaceutical and food industry. It is used to protect food products against microorganisms during storage, as a flavoring in the production of chocolate and confectionery products, as an important ingredient in fragrance, and as a preservative in medical products. However, little is known of its effect on insects. The main aim of this study was to determine the influence of benzyl alcohol on the defense systems of the wax moth Galleria mellonella, i.e., its cuticular lipid composition and critical elements of its immune system. A gas chromatography/mass spectrometry (GC/MS) analysis found benzyl alcohol treatment to elicit significant quantitative and qualitative differences in cuticular free fatty acid (FFA) profiles. Our findings indicate that benzyl alcohol treatment increased the levels of HSP70 and HSP90 and decreased those of HSF1, histamine, and cysteinyl leukotriene. Benzyl alcohol application also increased dismutase level in the hemolymph and lowered those of catalase and 8-OHdG. The treatment also had negative effects on G. mellonella hemocytes and a Sf9 cell line in vitro: 48-h treatment resulted in morphological changes, with the remaining cells being clearly spindle-shaped with numerous granules. The high insecticidal activity of compound and its lack of toxicity towards vertebrates suggest it could be an effective insecticide. [ABSTRACT FROM AUTHOR] more...

Copyright of Ziemniak Polski is the property of Instytutem Hodowli & Aklimatyzacji Roslin - Panstwowym Instytutem Badawczym and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...

In insects, the cell‐mediated immune response involves an active role of hemocytes in phagocytosis, nodulation, and encapsulation. Although these processes have been well documented in multiple species belonging to different insect orders, information concerning the immune response, particularly the hemocyte types and their specific function in the black soldier fly Hermetia illucens, is still limited. This is a serious gap in knowledge given the high economic relevance of H. illucens larvae in waste management strategies and considering that the saprophagous feeding habits of this dipteran species have likely shaped its immune system to efficiently respond to infections. The present study represents the first detailed characterization of black soldier fly hemocytes and provides new insights into the cell‐mediated immune response of this insect. In particular, in addition to prohemocytes, we identified five hemocyte types that mount the immune response in the larva, and analyzed their behavior, role, and morphofunctional changes in response to bacterial infection and injection of chromatographic beads. Our results demonstrate that the circulating phagocytes in black soldier fly larvae are plasmatocytes. These cells also take part in nodulation and encapsulation with granulocytes and lamellocyte‐like cells, developing a starting core for nodule/capsule formation to remove/encapsulate large bacterial aggregates/pathogens from the hemolymph, respectively. These processes are supported by the release of melanin precursors from crystal cells and likely by mobilizing nutrient reserves in newly circulating adipohemocytes, which could thus trophically support other hemocytes during the immune response. Finally, the regulation of the cell‐mediated immune response by eicosanoids was investigated. [ABSTRACT FROM AUTHOR] more...

This work presents a growth of Fe3O4 nanoparticle films on silicon substrate. The iron oxide is deposited applying a pulsed laser deposition technique. The process is performed in open air in the absence and presence of external magnetic field. In fact, the morphologies of the obtained Fe3O4–Si samples are similar. The Fe3O4 nanoparticles are spherical with average diameters of 30 nm and are densely agglomerated on the Si substrate. The Fe3O4–Si material prepared in the absence of magnetic field has revealed more intense signals during X‐ray diffraction and Raman measurements. The magnetic investigations indicate that the Fe3O4 nanoparticles are significantly coupled with the Si substrate and do not exhibit superparamagnetic behavior. Moreover, the Verwey transition is 98 K for both investigated Fe3O4–Si samples. [ABSTRACT FROM AUTHOR] more...

Copyright of Ziemniak Polski is the property of Instytutem Hodowli & Aklimatyzacji Roslin - Panstwowym Instytutem Badawczym and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) more...