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22 results on '"Kaddai, Vincent A."'

1. Ceramides Increase Fatty Acid Utilization in Intestinal Progenitors to Enhance Stemness and Increase Tumor Risk

Author

Li, Ying, Chaurasia, Bhagirath, Rahman, M. Mahidur, Kaddai, Vincent, Maschek, J. Alan, Berg, Jordan A., Wilkerson, Joseph L., Mahmassani, Ziad S., Cox, James, Wei, Peng, Meikle, Peter J., Atkinson, Donald, Wang, Liping, Poss, Annelise M., Playdon, Mary C., Tippetts, Trevor S., Mousa, Esraa M., Nittayaboon, Kesara, Anandh Babu, Pon Velayutham, Drummond, Micah J., Clevers, Hans, Shayman, James A., Hirabayashi, Yoshio, Holland, William L., Rutter, Jared, Edgar, Bruce A., and Summers, Scott A.

Published
2023
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2. Adipocyte Ceramides Regulate Subcutaneous Adipose Browning, Inflammation, and Metabolism

Author

Chaurasia, Bhagirath, Kaddai, Vincent Andre, Lancaster, Graeme Iain, Henstridge, Darren C., Sriram, Sandhya, Galam, Dwight Lark Anolin, Gopalan, Venkatesh, Prakash, K.N. Bhanu, Velan, S. Sendhil, Bulchand, Sarada, Tsong, Teh Jing, Wang, Mei, Siddique, Monowarul Mobin, Yuguang, Guan, Sigmundsson, Kristmundur, Mellet, Natalie A., Weir, Jacquelyn M., Meikle, Peter J., Bin M. Yassin, M. Shabeer, Shabbir, Asim, Shayman, James A., Hirabayashi, Yoshio, Shiow, Sue-Anne Toh Ee, Sugii, Shigeki, and Summers, Scott A.

Published
2016
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4. Serine Palmitoyltransferase Controls Stemness of Intestinal Progenitors

Author

Li, Ying, primary, Chaurasia, Bhagirath, additional, Kaddai, Vincent, additional, Wilkerson, Joseph L., additional, Alan Maschek, J., additional, Cox, James, additional, Wei, Peng, additional, Bensard, Claire, additional, Meikle, Peter J, additional, Clevers, Hans, additional, Shayman, James A, additional, Hirabayashi, Yoshio, additional, Holland, William L., additional, Rutter, Jared, additional, and Summers, Scott A., additional

Published
2020
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8. Corrigendum to 'Short chain fatty acids induce UCP2-mediated autophagy in hepatic cells' [Biochem. Biophys. Res. Commun. 480 (2016) 461–467] (S0006291X16317508) (10.1016/j.bbrc.2016.10.072)

Author

Iannucci, Liliana F., Sun, Jingfeng, Singh, Brijesh K., Zhou, Jin, Kaddai, Vincent A., Yen, Paul M., Sinha, Rohit A., LANNI, Antonia, Iannucci, Liliana F., Sun, Jingfeng, Singh, Brijesh K., Zhou, Jin, Kaddai, Vincent A., Lanni, Antonia, Yen, Paul M., and Sinha, Rohit A.

Subjects
Biophysic, Cell Biology, Biochemistry, Molecular Biology
Abstract

The authors regret for typographical errors in the Figure legends 1 and 2 where MAP1MAP1LC3 has been now replaced by MAP1LC3. Also there was a missing text added in the Figure legend 3E. Corrections were done in the Material and methods section at page 462 where T3 was replaced with SCFAs and 13 weeks with 12 weeks.

Published
2017

9. Targeting a ceramide double bond improves insulin resistance and hepatic steatosis

Author

Chaurasia, Bhagirath, primary, Tippetts, Trevor S., additional, Mayoral Monibas, Rafael, additional, Liu, Jinqi, additional, Li, Ying, additional, Wang, Liping, additional, Wilkerson, Joseph L., additional, Sweeney, C. Rufus, additional, Pereira, Renato Felipe, additional, Sumida, Doris Hissako, additional, Maschek, J. Alan, additional, Cox, James E., additional, Kaddai, Vincent, additional, Lancaster, Graeme Iain, additional, Siddique, Monowarul Mobin, additional, Poss, Annelise, additional, Pearson, Mackenzie, additional, Satapati, Santhosh, additional, Zhou, Heather, additional, McLaren, David G., additional, Previs, Stephen F., additional, Chen, Ying, additional, Qian, Ying, additional, Petrov, Aleksandr, additional, Wu, Margaret, additional, Shen, Xiaolan, additional, Yao, Jun, additional, Nunes, Christian N., additional, Howard, Andrew D., additional, Wang, Liangsu, additional, Erion, Mark D., additional, Rutter, Jared, additional, Holland, William L., additional, Kelley, David E., additional, and Summers, Scott A., additional

Published
2019
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14. CD2AP, Rabip4, and Rabip4':analysis of interaction with Rab4a and regulation of endosomes morphology

Author

Monzo, Pascale, Mari, Muriel, Kaddai, Vincent, Gonzalez, Teresa, Le Marchand-Brustel, Yannick, Cormont, Mireille, Faculteit Medische Wetenschappen/UMCG, and Center for Liver, Digestive and Metabolic Diseases (CLDM)

Subjects
rab4 GTP-Binding Proteins, Proteins, Endosomes, Saccharomyces cerevisiae, Endosomes/metabolism, Glutathione, Guanine Nucleotides, Glutathione/metabolism, Cytoskeletal Proteins, rab4 GTP-Binding Proteins/isolation & purification, Two-Hybrid System Techniques, Proteins/metabolism, Guanine Nucleotides/metabolism, Saccharomyces cerevisiae/enzymology, Adaptor Proteins, Signal Transducing, Protein Binding
Abstract

In this chapter, we describe various approaches that allow us to study interactions between the small GTPase Rab4a and its two effectors, Rabip4 and CD2AP. Two complementary approaches, one using the yeast two-hybrid system and the other using a GST pull-down assay, are described. We document the studies of the localization of these proteins by cellular fractionation. Finally, we develop cellular imaging techniques to study the morphology of vesicular structures containing Rab4a. We show that the coexpression of Rab4a with its effectors affects Rab4a-containing structures, giving a clear indication of their interaction in the mammalian cellular context.

Published
2005

15. A Role for Ceramides, but Not Sphingomyelins, as Antagonists of Insulin Signaling and Mitochondrial Metabolism in C2C12 Myotubes.

Author

Min Park, Kaddai, Vincent, Jianhong Ching, Fridianto, Kevin T., Sieli, Ryan J., Shigeki Sugii, and Summers, Scott A.

Subjects
*CERAMIDES, *SPHINGOMYELIN, *CELLULAR signal transduction, *MITOCHONDRIA, *CELL metabolism
Abstract

The accumulation of sphingolipids in obesity leads to impairments in insulin sensitivity and mitochondrial metabolism, but the precise species driving these defects is unclear. We have modeled these obesity-induced effects in cultured C2C12 myotubes, using BSA-conjugated palmitate to increase synthesis of endogenous sphingolipids and to inhibit insulin signaling and oxidative phosphorylation. Palmitate (a) induced the accumulation of sphingomyelin (SM) precursors such as sphinganine, dihydroceramide, and ceramide; (b) inhibited insulin stimulation of a central modulator of anabolic metabolism, Akt/PKB; (c) inhibited insulin-stimulated glycogen synthesis; and (d) decreased oxygen consumption and ATP synthesis. Under these conditions, palmitate failed to alter levels of SMs, which are the most abundant sphingolipids, suggesting that they are not the primary intermediates accounting for the deleterious palmitate effects. Treating cells with a pharmacological inhibitor of SM synthase or using CRISPR to knock out the Sms2 gene recapitulated the palmitate effects by inducing the accumulation of SM precursors and impairing insulin signaling and mitochondrial metabolism. To profile the sphingolipids that accumulate in obesity, we performed lipidomics on quadriceps muscles from obese mice with impaired glucose tolerance. Like the cultured myotubes, these tissues accumulated ceramides but not SMs. Collectively, these data suggest thatSMprecursors such as ceramides, rather than SMs, are likely nutritional antagonists of metabolic function in skeletal muscle. [ABSTRACT FROM AUTHOR]

Published
2016
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18. Viral genotype-specific role of PNPLA3 , PPARG , MTTP, and IL28B in hepatitis C virus-associated steatosis

Author

Cai, Tao, primary, Dufour, Jean-François, additional, Muellhaupt, Beat, additional, Gerlach, Tilman, additional, Heim, Markus, additional, Moradpour, Darius, additional, Cerny, Andreas, additional, Malinverni, Raffaele, additional, Kaddai, Vincent, additional, Bochud, Murielle, additional, Negro, Francesco, additional, and Bochud, Pierre-Yves, additional

Published
2011
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22. Short chain fatty acids induce UCP2-mediated autophagy in hepatic cells

Author

Rohit A. Sinha, Liliana F. Iannucci, Antonia Lanni, Jingfeng Sun, Jin Zhou, Paul M. Yen, Brijesh K. Singh, Vincent A. Kaddai, Iannucci, Liliana F., Sun, Jingfeng, Singh, Brijesh K., Zhou, Jin, Kaddai, Vincent A., Lanni, Antonia, Yen, Paul M., and Sinha, Rohit A.

Subjects
Male, UCP2, 0301 basic medicine, Biophysics, Butyrate, Gut flora, Biochemistry, Cell Line, Mice, 03 medical and health sciences, Autophagy, Animals, Uncoupling Protein 2, Protein kinase A, Molecular Biology, Cells, Cultured, biology, Fatty Acids, AMPK, Cell Biology, SCFA, biology.organism_classification, Gastrointestinal Microbiome, Cell biology, Mice, Inbred C57BL, Butyrates, 030104 developmental biology, Hepatocytes, Hepatic stellate cell, Microbiome, Propionates, Flux (metabolism), Intracellular
Abstract

Short-chain fatty acids (SCFAs) are gut microbial fermentation products derived from dietary fiber sources. Although depletion of gut microflora has been linked to the development of liver disease, the direct effects of SCFAs on intracellular hepatic processes are not well understood. In this study, we demonstrated that the SCFAs, propionate and butyrate, regulated autophagic flux in hepatic cells in a cell-autonomous manner. Induction of autophagy by SCFAs required PPARγ stimulation of Uncoupling Protein 2 (UCP2) expression that was associated with reduced intracellular ATP levels and activation of PRKAA1/AMPK (protein kinase, AMP-activated, alpha 1 catalytic subunit). In addition, elimination of gut flora by chronic antibiotic treatment diminished basal hepatic autophagy in mice suggesting that gut microbiota can regulate hepatic autophagy. These findings provide novel insights into the interplay between diet, gut microbiota, short chain fatty acids, and hepatic autophagic signaling.

Published
2016

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