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5. A model phage display subtraction method with potential for analysis of differential gene expression.

Author

Stausbøl-Grøn B, Wind T, Kjaer S, Kahns L, Hansen NJ, Kristensen P, and Clark BF

Subjects
Animals, Antibody Specificity, Cell Line, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin Variable Region, Melanoma, Protein Biosynthesis, Tumor Cells, Cultured, Antibodies, Antigens analysis, Bacteriophages, Gene Expression, Information Systems, Proteins analysis
Abstract

In order to establish a subtractive procedure that makes it possible to enrich selectively phage displayed antibodies directed against proteins constituting a difference between two populations of cells, a competitive selection strategy utilising two solid phases was developed and tested. Antibodies recognising a defined difference between two otherwise identical protein mixtures were isolated and their specificity confirmed. To test further the efficacy of selection inhibition during the competitive selections, selections towards a total cell extract were performed with and without competition from the same extract. An analysis of the resulting phage antibodies confirmed the subtractive nature of the system described.

Published
1996
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