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Abstract Background This study aimed to compare the effects of two 12-week training intervention experimental ball games combined with standard behavioral rehabilitation against a control group solely utilizing standard behavioral rehabilitation on social communication impairments (SCI) in preschool children with Autism Spectrum Disorder (ASD). Methods A multi-arm controlled study design was implemented, involving 41 children diagnosed with ASD (mean age: 4.99 ± 0.76 years). 41 participants were randomized assigned to two experimental groups and a control group, The experimental group carried out ball combination training program group (BCTP) and mini-basketball training program group (MBTP) on the basis of routine behavioral rehabilitation, which underwent 12-week training interventions 5 times a week. The control group (n = 14) received only standard behavioral rehabilitation. Evaluations were conducted before and after interventions using the Social Responsiveness Scale, Second Edition (SRS-2). Results The results suggest that both 12-week interventions, BCTP, and MBTP, led to significant improvements in social communication impairment among children with ASD (p

Abstract Investigating proton transport at the interface in an excited state facilitates the mechanistic investigation and utilization of nanomaterials. However, there is a lack of suitable tools for in-situ and interfacial analysis. Here we addresses this gap by in-situ observing the proton transport of graphene quantum dots (GQDs) in an excited state through reduction of magnetic resonance relaxation time. Experimental results, utilizing 0.1 mT ultra-low-field nuclear magnetic resonance relaxometry compatible with a light source, reveal the light-induced proton dissociation and acidity of GQDs’ microenvironment in the excited state (Hammett acidity function: –13.40). Theoretical calculations demonstrate significant acidity enhancement in –OH functionalized GQDs with light induction ( $${{\mathrm{p}}}{K}_{{\text{a}}}^{*}$$ p K a * = –4.62, stronger than that of H2SO4). Simulations highlight the contributions of edge and phenolic –OH groups to proton dissociation. The light-induced superacidic microenvironment of GQDs benefits functionalization and improves the catalytic performances of GQDs. Importantly, this work advances the understanding of interfacial properties of light-induced sp 2–sp 3 carbon nanostructure and provides a valuable tool for exploring catalyst interfaces in photocatalysis.

Abstract Although hydrophobic interactions provide the main driving force for initial peptide aggregation, their role in regulating suprastructure handedness of higher-order architectures remains largely unknown. We here interrogate the effects of hydrophobic amino acids on handedness at various assembly stages of peptide amphiphiles. Our studies reveal that relative to aliphatic side chains, aromatic side chains set the twisting directions of single β-strands due to their strong steric repulsion to the backbone, and upon packing into multi-stranded β-sheets, the side-chain aromatic interactions between strands form the aromatic ladders with a directional preference. This ordering not only leads to parallel β-sheet arrangements but also induces the chiral flipping over of single β-strands within a β-sheet. In contrast, the lack of orientational hydrophobic interactions in the assembly of aliphatic peptides implies no chiral inversion upon packing into β-sheets. This study opens an avenue to harness peptide aggregates with targeted handedness via aromatic side-chain interactions.

The field of construction labor productivity (CLP) has witnessed a remarkable growth in scholarly research, presenting both opportunities and challenges due to the diverse focus and exponential increase in literature. This study aims to systematically review the burgeoning body of CLP literature, proposing an approach to tackle the complexity of the domain. Utilizing the text mining technique of Hierarchical Latent Dirichlet Allocation (HLDA), an automatic clustering method was developed to analyze and categorize the corpus of CLP research. The methodology involved a comprehensive extraction of 591 scholarly articles from scientific databases. These articles, spanning from 1973 to 2023, were subjected to HLDA topic modeling. This process generated a detailed three-layer, tree-like topic model, comprising three primary topics and 26 sub-topics, organized through the nested Chinese restaurant process (nCRP). The study advances theoretical and practical understanding by applying hierarchical topic modeling to construction project management literature and identifying key industry challenges.

TC4/DP780 dissimilar metals were laser welded with different thicknesses of the H62 interlayer. Joints with good forming and mechanical properties were obtained. The corrosive behaviors of TC4/DP780 joints in 3.5 wt% NaCl solution were investigated by micro-area scanning vibrating electrode technique (SVET). Results showed that TC4/DP780 joints effectively hindered the brittle intermetallic compounds of Ti–Fe through mutual diffusion and the solid solution between the H62 layer metal and TC4 alloy. Cu–Ti intermetallic compounds and H62 Cu-based solid solution formed at the H62/TC4 interface. The intermetallic compounds layer grew dendritically from the dependent TC4 alloy toward the weld. With increased Ti element content and interlayer temperature, the microstructure morphology changed from fine dendrites to coarse dendrites. Corrosion experiments further revealed that with increased thickness of the H62 interlayer, the corrosion resistance of joints initially increased and then decreased. The joints added with a 0.4 mm H62 interlayer had the highest self-corrosion potential and the lowest self-corrosion current density. The corrosion rate of joints was the slowest. DP780 steel formed loose porous Fe2O3, accelerating the metal-corrosion dissolution. The H62 primarily formed due to the corrosion susceptibility of ZnO, leaving the porous structure of the Cu layer and the enabling the formation of CuO. TC4 had the highest corrosion potential and was protected as the cathode. TC4 underwent galvanic coupling corrosion with the DP780 steel and the H62 interlayer metal. Meanwhile, TC4 was oxidized in air to form a dense TiO2 and Al2O3 protective film, which had good restoration and passivation effects.

Fusarium crown rot (FCR) is one of the most important soilborne diseases affecting wheat production. To investigate the diversity of the pathogens causing this disease, 199 diseased wheat samples were collected from 13 cities in Shandong province. In total, 468 isolates were obtained, and from these isolates, 11 Fusarium species were identified based on phylogenetic analyses with the translation elongation factor-1α (TEF-1α), RNA polymerase II largest subunit (RPB1), and RNA polymerase II second largest subunit (RPB2) gene sequences. Of these Fusarium isolates, 283 were identified as Fusarium pseudograminearum and the remaining isolates were identified as Fusarium graminearum (n = 113), Fusarium sinensis (n = 28), Fusarium acuminatum (n = 18), Fusarium incarnatum (n = 13), Fusarium ipomoeae (n = 5), Fusarium flocciferum (n = 3), Fusarium proliferatum (n = 2), Fusarium asiaticum (n = 1), Fusarium culmorum (n = 1), and Fusarium oxysporum (n = 1), suggesting that F. pseudograminearum is the dominant pathogen of FCR of wheat in Shandong province. Pathogenicity tests demonstrated that all 11 Fusarium species could cause typical symptoms of FCR on wheat seedlings. The results of the study indicate that a greater diversity of Fusarium species can cause FCR of wheat in Shandong province than that has been previously reported. This is the first report in the world of Fusarium incarnatum, Fusarium ipomoeae, and Fusarium flocciferum as pathogens causing FCR in wheat.

Abstract Community partitioning is an effective technique for cyberspace mapping. However, existing community partitioning algorithm only uses the topological structure of the network to divide the community and disregards factors such as real hierarchy, overlap, and directionality of information transmission between communities in cyberspace. Consequently, the traditional community division algorithm is not suitable for dividing cyberspace resources effectively. Based on cyberspace community structure characteristics, this study introduces an algorithm that combines an improved local fitness maximization (LFM) algorithm with the PageRank (PR) algorithm for community partitioning on cyberspace resources, called PR-LFM. First, seed nodes are determined using degree centrality, followed by local community expansion. Nodes belonging to multiple communities undergo further partitioning so that they are retained in the community where they are most important, thus preserving the community’s original structure. The experimental data demonstrate good results in the resource division of cyberspace.

Three different laser welding methods were used to welding 2 mm DP980 steel butt joint, which were laser welding, laser-TIG hybrid welding and laser powder feeding (CoCrNi) welding respectively. An observational analysis was carried out in terms of microstructure, mechanical properties and corrosion resistance. It was discovered that a central microstructure of laser welding and laser-TIG hybrid welding the weld was dominated by slatted martensite. The laser powder feeding welding has resulted in a remarkable reduction of cylindrical crystals and an increased number of isometric crystals in weld center due to the addition of CoCrNi powder. Microstructure was further refined in incomplete phase transition zone by three different laser welding methods. The tempered martensite was also formed in the tempering zone, which softened the joint. The hardness values decreased considerably and tensile samples were also fractured at this location. Comparison with corrosion resistance of their joints through polarization curves and impedance spectra shows that laser powder feeding joint > laser joint > laser-TIG hybrid joint. The corrosion resistance of laser and laser-TIG hybrid joints in the base metal (BM) > heat affected zone (HAZ) > weld zone (WZ) is shown based on scanning vibrating electrode technique (SVET) localized area testing data at different time intervals. The laser powder feeding welding enhances the density of passivation film by reacting Cr to form Cr2O3, thereby improving corrosion resistance of entire joint.

The marine carbonates in the Ordovician Majiagou Formation in the Ordos Basin have significant exploration potential. Research has focused on their thermal history and hydrocarbon accumulation stages, as these are essential for guiding the exploration and development of hydrocarbons. In this paper, we study the thermal evolution history of the carbonate reservoirs of the Ordovician Majiagou Formation in the east-central Ordos Basin. Furthermore, petrographic and homogenization temperature studies of fluid inclusions were carried out to further reveal the hydrocarbon accumulation stages. The results demonstrate that the degree of thermal evolution of the Ordovician carbonate reservoirs is predominantly influenced by the deep thermal structure, exhibiting a trend of high to low values from south to north in the central region of the basin. The Fuxian area is located in the center of the Early Cretaceous thermal anomalies, with the maturity degree of the organic matter ranging from 1 to 3.2%, with a maximum value of 3.2%. The present geothermal gradient of the Ordovician Formation exhibits the characteristics of east–high and west–low, with an average of 28.6 °C/km. The average paleo-geotemperature gradient is 54.2 °C/km, the paleoheat flux is 55 mW/m2, and the maximum paleo-geotemperature reaches up to 270 °C. The thermal history recovery indicates that the Ordovician in the central part of the basin underwent three thermal evolution stages: (i) a slow warming stage before the Late Permian; (ii) a rapid warming stage from the end of the Late Permian to the end of the Early Cretaceous; (iii) a cooling stage after the Early Cretaceous, with the hydrocarbon production of hydrocarbon source rocks weakening. In the central part of the basin, the carbonate rock strata of the Majiagou Formation mainly developed asphalt inclusions, natural gas inclusions, and aqueous inclusions. The fluid inclusions can be classified into two stages. The early-stage fluid inclusions are mainly present in dissolution holes. The homogenization temperature is 110–130 °C; this coincides with the hydrocarbon charging period of 210–165 Ma, which corresponds to the end of the Triassic to the end of the Middle Jurassic. The late-stage fluid inclusions are in the dolomite vein or late calcite that filled the gypsum-model pores. The homogenization temperature is 160–170 °C; this coincides with the hydrocarbon charging period of 123–97 Ma, which corresponds to the late Early Cretaceous. Both hydrocarbon charging periods are in the rapid stratigraphic warming stage.

Purpose: This study aimed to: (i) analyze the changes in physical fitness and body composition following a 4-month intervention of small-sided games (SSG) training; and (ii) analyze the association between internal and external training loads and the observed changes in physical fitness and body composition among sedentary young adults. Methods: Sixty sedentary individuals (males: 30; females: 30) participated in this randomized controlled trial study. Physical fitness and body composition parameters were assessed at the 1st, 8th weeks, and 16th weeks after a SSG intervention. Results: Significant main effects of time and gender on overall physical fitness parameters, with a notable time-group interaction were observed. For body composition measures, we found significant main effects of time, group, and gender. Furthermore, we identified significant correlations between shuttle run, handgrip, and vertical jump performance, and the time spent at VO2max (TVO2max) during SSG (r = −0.779, p = 0.001; r = −0.788, p = 0.001; r = 0.692, p = 0.004, respectively). Handgrip strength exhibited significant correlations with heat exhaustion (HE) and total distance (TD) during SSG (r = −0.616, p = 0.014; r = −0.629, p = 0.012). Similarly, we observed significant correlations between hip perimeter (HP), skinfolds (SF), waist-to-hip ratio (W:H), and TVO2max (r = 0.624, p = 0.013; r = 0.663, p = 0.007; r = 0.535, p = 0.040, respectively). Conclusion: This study indicates that the intensity achieved during SSG plays a crucial role in fostering positive adaptations in aerobic capacity, maximal strength, and jumping performance in recreational soccer. Therefore, practitioners should ensure that SSG formats generate the required stimulus to sustain prolonged periods within VO2max zones.

Recreational small-sided games (SSGs) have demonstrated positive effects on body composition and physical fitness, while minimizing adverse outcomes. In this randomized controlled study, we aimed to investigate the impact of incorporating an additional 16-week intervention program involving recreational soccer SSGs on parameters related to body composition and physical fitness in sedentary young adult males and females. Sixty sedentary participants, with a mean age of 20.2 years, were randomly assigned to either the small-sided games group (SSG; n = 30) or the active control group, which participated in regular physical education classes (CG; n = 30). The SSG group engaged in the same activities as the control group but additionally participated in a recreational SSG football program. This program involved continuous and intermittent moderate-to high-intensity exercises conducted on 20 m × 30 m and 30 m × 50 m football fields. In contrast, the CG group received 1 h of physical education once a week. The interventions were administered for a duration of sixteen weeks. Baseline, 8-week, and post-intervention assessments were conducted to measure body mass (BM), body mass index (BMI), waist circumference (WC), hip circumference (HC), waist-to-hip ratio (WHR), skinfold thickness (SFT), standing broad jump (SBJ), vertical jump (VJ), handgrip strength (HG) for both left and right hands, shuttle run distance (SRD), and shuttle run estimated VO2max. Results indicated that both male and female participants in the SSG group exhibited significant improvements in BM, BMI, SFT, WC, HC, and WHR following the intervention (p 0.05). Additionally, SSG participants (regardless of sex) displayed significant enhancements in SBJ, VJ, HG, SRD, and VO2max (p 0.05). The findings from this experimental study suggested that a 16-week recreational soccer SSG intervention effectively enhanced body composition and physical fitness among overweight sedentary young adults, offering a pleasurable alternative to conventional training approaches.

This paper discusses the successful welding of AZ31 Mg alloy to 22MnB5 high-strength steel using pinless friction stir welding (P-FSSW) to join dissimilar metals and reduce vehicle weight while maintaining strength and stiffness. P-FSSW eliminates fusion welding defects, ensuring good performance of Mg/steel welds. An orthogonal experimental design was adopted to comprehensively analyze welding parameters for determining the optimum P-FSSW process conditions. The study provides an in-depth examination of the interfacial layer formation between AZ31 Mg alloy and 22MnB5 steel, emphasizing the importance of Al for effective bonding in Mg alloys. Numerical simulation of the welding process's temperature field is verified by thermocouple measurements, confirming result accuracy. The study aims to optimize bonding of dissimilar materials and facilitate interfacial reactions for achieving high-performance welded joints. This comprehensive understanding of the P-FSSW process and interfacial layer formation holds great significance for lightweight construction in the automotive industry, contributing to reduced emissions and improved fuel efficiency.

Wheat is a vital global food crop, yet it faces challenges in saline–alkali soils where Fusarium crown rot significantly impacts growth. Variations in wheat growth across regions are often attributed to uneven terrain. To explore these disparities, we examined well-growing and poorly growing wheat samples and their rhizosphere soils. Measurements included wheat height, root length, fresh weight, and Fusarium crown rot severity. Well-growing wheat exhibited greater height, root length, and fresh weight, with a lower Fusarium crown rot disease index compared to poorly growing wheat. Analysis of rhizosphere soil revealed higher alkalinity; lower nutrient levels; and elevated Na, K, and Ca levels in poorly growing wheat compared to well-growing wheat. High-throughput sequencing identified a higher proportion of unique operational taxonomic units (OTUs) in poorly growing wheat, suggesting selection for distinct fungal species under stress. FUNGuild analysis indicated a higher prevalence of pathogenic microbial communities in poorly growing wheat rhizosphere soil. This study underscores how uneven terrains in saline–alkali soils affect pH, nutrient dynamics, mineral content, wheat health, and rhizosphere fungal community structure.

The acetylation of histone lysine residues regulates multiple life processes, including growth, conidiation, and pathogenicity in filamentous pathogenic fungi. However, the specific function of each lysine residue at the N-terminus of histone H3 in phytopathogenic fungi remains unclear. In this study, we mutated the N-terminal lysine residues of histone H3 in Fusarium pseudograminearum, the main causal agent of Fusarium crown rot of wheat in China, which also produces deoxynivalenol (DON) toxins harmful to humans and animals. Our findings reveal that all the FpH3K9R, FpH3K14R, FpH3K18R, and FpH3K23R mutants are vital for vegetative growth and conidiation. Additionally, FpH3K14 regulates the pathogen’s sensitivity to various stresses and fungicides. Despite the slowed growth of the FpH3K9R and FpH3K23R mutants, their pathogenicity towards wheat stems and heads remains unchanged. However, the FpH3K9R mutant produces more DON. Furthermore, the FpH3K14R and FpH3K18R mutants exhibit significantly reduced virulence, with the FpH3K18R mutant producing minimal DON. In the FpH3K9R, FpH3K14R, FpH3K18R, and FpH3K23R mutants, there are 1863, 1400, 1688, and 1806 downregulated genes, respectively, compared to the wild type. These downregulated genes include many that are crucial for growth, conidiation, pathogenicity, and DON production, as well as some essential genes. Gene ontology (GO) enrichment analysis indicates that genes downregulated in the FpH3K14R and FpH3K18R mutants are enriched for ribosome biogenesis, rRNA processing, and rRNA metabolic process. This suggests that the translation machinery is abnormal in the FpH3K14R and FpH3K18R mutants. Overall, our findings suggest that H3 N-terminal lysine residues are involved in regulating the expression of genes with important functions and are critical for fungal development and pathogenicity.

The positioning of civil aviation aircraft relative to a geographic reference point on Earth in a Cartesian frame is significant to detect the deviations from the desired path, especially for high-altitude airports or special airports based on performance-based navigation (PBN). To obtain these critical deviations during aircraft approach and landing, it is fundamental to estimate the continuous flight variables and discrete flight modes simultaneously with enough accuracy. With the coordinate conversion between the North, East, and Down (NED) frame and the geographic coordinate system based on World Geodetic System 1984 (WGS-84) considered, this study proposed a non-linear stochastic hybrid estimation algorithm with adaptive square-root unscented particle filtering (ASR-UPF) to estimate the true path. The probabilities of mode transition, represented by the normal cumulative density function of continuous states, determine whether to proceed with mode transitions. In addition, the adaptive update characterized by tracking variable noise and the importance sampling distributions based on the results of square-root unscented Kalman filtering (SR-UKF), as a comparative study of continuous system filtering, were used. The experiments illustrated the ASR-UPF is able to reduce the state estimation error more effectively, and more promptly track the error caused by incorrect mode estimation with adaptability compared to the SR-UKF. A further test with real flight data indicates that the proposed method gives the refined estimation of position and azimuth in NED frame.

Reasonable control of grouting parameters was the key to improving the pile-soil interaction. However, quantitative analysis on the effect of grouting parameters on the pile-soil interaction was still lacking. This article developed an intelligent post-grouting system for precise control and quantitative analysis of grouting parameters. The conventional preparation method for shear tests were improved in this article. By changing the normal stress of the pile-soil interface during grouting, the test sample could simulate the grout distribution of the pile-soil interface at different depths. Through the improved experimental methods, a more accurate analysis of the mechanism for the pile-soil interaction was achieved. And the effectiveness of the intelligent post-grouting system was further verified by the case application. The results indicated that increasing the grout volume could improve the interface shear strength when the normal stress and grouting pressure were constant. This improvement effect caused by increasing grout volume decreased when the normal stress was high. Increasing the grouting pressure increased the interface shear strength when the normal stress and the grout volume remained constant. This improvement effect caused by increasing grouting pressure decreased when the grout volume was small. By appropriately controlling the two grouting parameters, the cohesion and the shear strength of the pile-soil interface were increased by 5.8–18.8 times and 1.16–2.91 times, respectively. The results indicated that the intelligent post-grouting system could accurately control the grouting parameters and effectively improve the pile-soil interaction of bored piles. The intelligent post-grouting system effectively reduced geotechnical engineering risks and improved the bearing capacity of pile.

Aerobic fitness is a critical aspect of overall health and well-being, essential for maintaining a high quality of life. Unfortunately, sedentary behavior has been on the rise among young adults, and this has had a negative impact on their aerobic fitness levels. Therefore, it is crucial to identify enjoyable physical exercise training programs that can play a pivotal role in improving aerobic fitness. The objective of this study was to systematically review the experimental studies concerning the impact of small-sided games (SSGs) training programs, implemented across various team sports, on the enhancement of aerobic fitness in a youth sedentary population. A literature search was conducted in PubMed, Scopus, and the Web of Science on August 01, 2023. Our eligibility criteria focused on studies involving sedentary youth populations (aged

Graph neural networks (GNNs) is applied successfully in many graph tasks, but there still exists a limitation that many of GNNs model do not consider uncertainty quantification of its output predictions. For uncertainty quantification, there are mainly two types of methods which are frequentist and Bayesian. But both methods need to sampling to gradually approximate the real distribution, in contrast, evidential deep learning formulates learning as an evidence acquisition process, which could get uncertainty quantification by placing evidential priors over the original Gaussian likelihood function and training the NN to infer the hyperparameters of the evidential distribution without sampling. So evidential deep learning (EDL) has its own advantage in measuring uncertainty. We apply it with diffusion convolutional recurrent neural network (DCRNN), and do the experiment in spatiotemporal forecasting task in a real-world traffic dataset. And we choose mean interval scores (MIS), a good metric for uncertainty quantification. We summarized the advantages of each method.

Wide hybridization is a strategy for broadening the genetic basis of wheat. Because an efficient method for inducing wheat–alien chromosome translocations will allow producing useful germplasm, it is desirable to discover new genes that induce chromosomal variation. In this study, chromosome 5P from A. cristatum was shown to induce many types of chromosomal structural variation in a common wheat background, including nonhomoeologous chromosome translocations, as revealed by genomic in situ hybridization, fluorescence in situ hybridization, and DNA marker analysis. Aberrant meiosis was associated with chromosomal structural variation, and aberrant meiotic behavior was observed in wheat–A. cristatum 5P monosomic and disomic addition lines, suggesting that the effect of chromosome 5P was independent of the number of chromosome 5P copies. Chromosome 5P disturbed homologous chromosome pairing at pachytene stage in a common wheat background, resulting in a high frequency of univalent formation and reduced crossing over. Thirteen genes involved in DNA repair or chromatin remodeling, including RAD52-like and MSH6 genes, were differentially expressed (upregulated) in wheat–A. cristatum 5P addition lines according to transcriptome analysis, implicating chromosome 5P in the process of meiotic double-strand break repair. These findings provide a new, efficient tool for inducing wheat–alien chromosome translocations and producing new germplasm.

Knorringia sibirica is a typical species of Polygonaceae with high medicinal and ecological value. However, there are few available phylogenetic and genetic studies about the chloroplast genome of K. sibirica. In the current study, a comprehensive examination of the chloroplast genome of K. sibirica was performed. The K. sibirica chloroplast genome is 161,384 bp, circular with a typical quadripartite structure, and comprised of guanine + cytosine content of 37.63%. The genome consists of 131 genes, including 86 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Seventy simple sequence repeats were identified in the genome. When compared with three closely related species, the result revealed that the chloroplast genome of K. sibirica was conserved in genome composition and structure. The specific sites in the genome suitable for DNA barcodes were identified by calculation of the nucleotide diversity. Finally, a phylogenetic tree of 49 species in Polygonaceae was constructed using the whole chloroplast genome. The phylogenetic analysis suggested that Knorringia is an independent genus in the Polygonea tribe. This research could provide support for the utilization of genetic sources and the evolutionary study of K. sibirica.

The stacked and brittle 2D layered structure of molybdenum disulphide limits its practical application in lithium ion batteries. Here, authors report a dewetting-induced manufacture strategy to create the interpenetrating network and induce the pseudocapacity to improve the electrochemical performance.

Biological seed dressing is a cost-effective means to protect plant roots from pathogens. Trichoderma is generally considered as one of the most common biological seed dressings. However, there is still a dearth of information on the effects of Trichoderma on microbial community of rhizosphere soil. High-throughput sequencing was used to analyze the effects of Trichoderma viride and a chemical fungicide on microbial community of soybean rhizosphere soil. The results showed that both T. viride and chemical fungicide could significantly reduce the disease index of soybean (15.11% for Trichoderma and 17.33% for Chemical), while no significant difference was observed between them. Both T. viride and chemical fungicide could affect the structure of rhizosphere microbial community, they increased the β-diversity of microbial community and significantly reduce the relative abundance of Saprotroph-Symbiotroph. Chemical fungicide could reduce the complexity and stability of co-occurrence network. However, T. viride is beneficial for maintaining network stability and increasing network complexity. There were 31 bacterial genera and 21 fungal genera significantly correlated with the disease index. Furthermore, several potential plant pathogenic microorganisms were also positively correlated with disease index, such as Fusarium, Aspergillus, Conocybe, Naganishia, and Monocillium. From this work, T. viride may be used as a substitute for chemical fungicide to control soybean root rot and be more friendly to soil microecology.

Aqueous Zn–CO2 battery possesses a large theoretical capacity of 820 mAh g-1 (5855 mAh cm-3) and high safety, showing a unique position in carbon neutrality and/or reduction and energy conversion and storage, which has developed rapidly in recent years. However, obstacles such as low value-added products, low current density, high overvoltage, and finite cycles impede its practical application. Cathode catalysts, as a key component, have a significant influence on gas cell performance. Despite many updated papers on cathode materials for aqueous Zn–CO2 batteries, a systematic summary has rarely been reported, and even less is mentioned about the design principle and development strategy for efficient catalysts. Relying on the structure and mechanism of the Zn–CO2 battery, this review discusses the research progress and existing challenges, and, more importantly, the design strategies and preparation methods of the efficient cathode are proposed, centering on material structure, charge distribution, and coordination environment. Finally, in this review, the opportunities for the development of a high-performance Zn–CO2 battery are highlighted, which enables enlightening the future exploration of next-generation energy storage systems.

Microswimmers are ubiquitous in nature and present highly-dynamic collective motion. This study presents computational simulations of spheroidal model-microswimmers confined between two walls, revealing two collective dynamic states, giant motile clusters and active turbulence, depending on density and hydrodynamic interactions.

Exercise addiction refers to maladaptive exercise patterns involving compulsivity and addiction-like behaviors. Exercise addiction has been found to relate to negative physical and mental health outcomes such as heart abnormalities, physical injuries, and interpersonal conflicts. Based on the social cognitive theory, this study investigated the extent to which the interplay of obsessive compulsive personality disorder (OCPD) traits and self-efficacy beliefs would influence the development and maintenance of exercise addiction. A total of 1228 college students in the United States responded to an online survey. Based on cut-off scores of the Revised Exercise Addiction Inventory, the prevalence estimates of exercise addiction were 4.0% for males and 1.8% for females. Results showed that males are more prone to exercise addiction than females. Also, OCPD traits and self-efficacy significantly predicted exercise addiction after controlling for age and sex. Self-efficacy acted as a moderator in influencing the relationship between OCPD traits and exercise addiction, especially for females. At high levels of self-efficacy, more OCPD traits were significantly associated with a higher risk of exercise addiction. However, at low levels of self-efficacy, there was no association between OCPD traits and exercise addiction. The findings suggest that public education and intervention for exercise addiction should attend to the interplay between personality factors and sex.

The spread of infections and rot are crucial factors in the decrease in tomato production. Accurately segmenting the affected tomatoes in real-time can prevent the spread of illnesses. However, environmental factors and surface features can affect tomato segmentation accuracy. This study suggests an improved YOLOv8s-Seg network to perform real-time and effective segmentation of tomato fruit, surface color, and surface features. The feature fusion capability of the algorithm was improved by replacing the C2f module with the RepBlock module (stacked by RepConv), adding SimConv convolution (using the ReLU function instead of the SiLU function as the activation function) before two upsampling in the feature fusion network, and replacing the remaining conventional convolution with SimConv. The F1 score was 88.7%, which was 1.0%, 2.8%, 0.8%, and 1.1% higher than that of the YOLOv8s-Seg algorithm, YOLOv5s-Seg algorithm, YOLOv7-Seg algorithm, and Mask RCNN algorithm, respectively. Meanwhile, the segment mean average precision (segment mAP@0.5) was 92.2%, which was 2.4%, 3.2%, 1.8%, and 0.7% higher than that of the YOLOv8s-Seg algorithm, YOLOv5s-Seg algorithm, YOLOv7-Seg algorithm, and Mask RCNN algorithm. The algorithm can perform real-time instance segmentation of tomatoes with an inference time of 3.5 ms. This approach provides technical support for tomato health monitoring and intelligent harvesting.

The Ordovician system of the Ordos Basin is rich in oil and gas resources, but the tectonic evolution of Ordovician strata and controls on natural gas generation and accumulation are poorly understood. In this study, we integrate field observations, seismic and drill core interpretation, and geochemical data analysis [total organic carbon (TOC) and vitrinite reflectance (Ro)] to address this issue. Results show a clear depositional axis, with thick (>2500 m) Ordovician sediments in the southern half of the basin, and thinner strata in the central and northern parts. Rovalues range from 2.04% to 3.45%, and 55 of 73 samples have TOC > 0.23 wt%. Our novel findings made as below: (i) Ordovician strata have high contents of organic matter and that thermal evolution reached the dry gas stage, favorable for natural gas generation. (ii) Ordovician strata have recorded multiple tectonic events and experienced erosion that resulted in the formation of a karst type residuum in the central and northeastern parts of the basin. (iii) An Early Cretaceous tectono-thermal event controlled the generation and accumulation of natural gas, and this was the peak period of gas generation of Ordovician strata. (iv) Natural gas accumulation and adjustment occurred during the Cretaceous and Cenozoic, respectively. The Ordos Basin has experienced strong deformation and denudation since the Late Cretaceous. Collectively, the deformation was more intense in the south and weaker in the north, preventing hydrocarbon preservation in the southern part of the basin. These findings provide new insights to guide oil and gas exploration of the Ordos Basin.

The characteristics and preparation methods of zeolite-based adsorbents and membranes were reviewed and their applications in gas separation and purification were introduced according to classification. The effects of framework structure, equilibrium cations and pore size of zeolites as well as temperature and pressure of the system on gas adsorption and separation were discussed, and the separation mechanisms were also summarized. The main defects and improved methods of zeolite-based adsorbents and membranes were briefly described, and their future trend for gas separation and purification was finally prospected.

Wild relatives of wheat are essential gene pools for broadening the genetic basis of wheat. Chromosome rearrangements and genomic variation in alien chromosomes are widespread. Knowledge of the genetic variation between alien homologous chromosomes is valuable for discovering and utilizing alien genes. In this study, we found that 5113 and II-30-5, two wheat–A. cristatum 6P addition lines, exhibited considerable differences in heading date, grain number per spike, and grain weight. Genome resequencing and transcriptome analysis revealed significant differences in the 6P chromosomes of the two addition lines, including 143,511 single-nucleotide polymorphisms, 62,103 insertion/deletion polymorphisms, and 757 differentially expressed genes. Intriguingly, genomic variations were mainly distributed in the middle of the chromosome arms and the proximal centromere region. GO and KEGG analyses of the variant genes and differentially expressed genes showed the enrichment of genes involved in the circadian rhythm, carbon metabolism, carbon fixation, and lipid metabolism, suggesting that the differential genes on the 6P chromosome are closely related to the phenotypic differences. For example, the photosynthesis-related genes PsbA, PsbT, and YCF48 were upregulated in II-30-5 compared with 5113. ACS and FabG are related to carbon fixation and fatty acid biosynthesis, respectively, and both carried modification variations and were upregulated in 5113 relative to II-30-5. Therefore, this study provides important guidance for cloning desirable genes from alien homologous chromosomes and for their effective utilization in wheat improvement.

Deep and ultra-deep layers in the petroliferous basins of China are characterized by large temperature difference and complicated thermal evolution history. The control effects of temperature and thermal evolution history on the differences of hydrocarbon phase states and the hydrocarbon generation history in deep and ultra-deep layers are researched less and unsystematically. To deal with this situation, based on a large number of temperature and pressure data of deep layers and combined with the complicated historical situation of deep layer evolution in the oil and gas basins of China, the effects of temperature, heating time and pressure on the hydrocarbon formation temperature and phase state were analyzed, and the types of temperature and pressure relationships were classified. Finally, based on the classification of thermal evolution history of deep and ultra-deep layers, We discussed the control effects of basin thermal evolution history on the hydrocarbon generation and phase state, and the following research results were obtained. First, the hydrocarbon phase states of deep layers in different basins and regions are greatly different, and they are mainly affected by temperature, heating time, heating rate, pressure, source rock types and other factors. And temperature is the most important factor controlling hydrocarbon generation and phase state distribution. Second, under the conditions of rapid temperature increasing and short heating time, there still may be oil reservoirs and condensate gas reservoirs in deep and ultra-deep layers in the case of high temperature. Third, overpressure inhibits hydrocarbon generation and pyrolysis. Fourth, there is a close relationship between temperature and formation pressure of deep layers, which can be divided into three types, i.e., low–medium temperature and high pressure type, high temperature and high pressure type, and medium temperature and low–medium pressure type. Fifth, the thermal evolution history of deep and ultra-deep layers can be divided into four types, namely the late rapid subsidence, heating and low geothermal gradient type, the late rapid subsidence, heating and high geothermal gradient type, the middle–late rapid heating and late uplifting and cooling type, and the early great subsidence and rapid heating and middle–late great uplift erosion and cooling type. In conclusion, deep and ultra-deep layers in the basins with different types of thermal history are different in hydrocarbon phase states, accumulation stages and prospects.

Developing facile strategies to realize the precise construction of Ni-Fe structures is of significance for water oxidation. Here, the authors demonstrate a universal microorganism-assisted corrosion strategy for preparing highly efficient Ni-Fe composites towards oxygen evolution.

The effects of different heat treatments on the microstructure and corrosion behavior of an as-cast ZK60 magnesium alloy were investigated by microscopic analyses, weight loss tests, hydrogen evolution tests, and electrochemical measurements. The results indicate that solution treatment (T4) could result in the dissolution of most of the second-phase particles and make the alloying elements (Zn and Zr) distribute uniformly. However, new tiny second phase particles re-precipitated during aging treatment (T6). The as-cast and heat-treated ZK60 alloys displayed filiform-like corrosion morphologies with different initiation and propagation features in the early corrosion period, and they gradually developed corrosion pits. Their corrosion rates showed similar change tendencies, and the T4-treated ZK60 alloy exhibited the best anti-corrosion performance owing to it having the weakest micro-galvanic effect. The relationship between the alloys’ microstructures and their corrosion features was analyzed, and the involved mechanism was examined.

We aimed to examine the therapeutic potential of polysaccharide H-1-2, a bioactive component of Pseudostellaria heterophylla, against pancreatic cancer, as well as to demonstrate the underlying molecular mechanisms. Invasion and migration of pancreatic cells treated with H-1-2 were evaluated. A xenograft tumor mouse model was established to assess the effect of H-1-2 on tumor growth. Expression levels of hypoxic inducible factor-1α (HIF1α) and anterior gradient 2 (AGR2) were measured in pancreatic cells after H-1-2 treatment. Luciferase report and chromatin immunoprecipitation assays were conducted to investigate HIF1α regulation on AGR2. AGR2 expression was re-introduced into pancreatic cells to assess the role of AGR2 as a downstream effector of hypoxia after H-1-2 treatment. H-1-2 inhibited invasion and migration of pancreatic cancer cells, repressed xenograft pancreatic tumor growth, and increased survival of mice. H-1-2 repressed AGR2 expression in pancreatic cancer cells through the hypoxia response element (HRE) in its promoter region. Ectopic AGR2 expression partially negated the H-1-2 inhibitory effect on invasion and migration of pancreatic cells and on xenograft pancreatic tumors growth, and it also compromised the H-1-2 promotional effect on survival of mice. We conclude that H-1-2 suppresses pancreatic cancer by inhibiting hypoxia-induced AGR2 expression, supporting further investigation into its efficacy against pancreatic cancer in clinical settings.

This study aimed to explore the impact of ozonated water (OW) disinfestation on soil fungal community composition in continuous ginger field. All soil samples were collected in continuous ginger field. There were two groups and 5 time points (0, 1, 3, 5, 9 day) in our study, including OW disinfestation treatment group (O3 group) and control group (CK group). Via internal transcribed spacer (ITS) sequencing and further analysis, the changes of fungal community composition were determined. As a result, at 0 and 9 days after aeration, the operational taxonomic units (OTUs) in O3 group were significantly higher than that in CK group. Compared with the CK group, in O3 group: the ACE and Chao1 index significantly increased on day 1, and the Shannon index significantly decreased while Simpson index significantly increased on day 0 after aeration. In O3 group, there were dynamic changes of top 10 abundance fungi from the genus-level and the growth of Trichoderma and Rhodotorula had been promoted while Hannaella was inhibited. In conclusion, OW disinfestation had complicated impacts on fungal communities in continuous ginger fields. The growth of Trichoderma and Rhodotorula has been promoted during disinfestation, which provided more reference information for soil OW disinfestation research.

Zinc–air batteries have received increasing attention in energy storage and conversion technologies. However, several challenges still emerge in the development of high‐level zinc–air batteries. In this regard, electrospun materials with unique nanostructures and characteristics are applied in the high‐performance zinc–air batteries. This work reviews recent progress of electrospun technologies for their diverse application in novel zinc anodes, separators, and air cathodes in zinc–air batteries. After a brief introduction, the fundamental principle and technological parameter of electrospinning technology are discussed, and then multifunctional electrospun nanofiber materials including their fabrication procedures, structures, and electrochemical properties are reviewed in the application of zinc–air batteries. Finally, urgent challenges and opportunities are comprehensively proposed for the electrospun nanomaterials in zinc–air batteries. This work intends to offer readable report for electrospun technology toward their application in zinc–air batteries, which inspires more fabrication approaches and material innovations in energy conversion and storage technologies.

The Mesozoic Chang 8 Section in the Zhenjing area is a typical low permeability-tight sand reservoir and is regarded as the most important set of paybeds in the study area. Guided by the principles of basic geological theory, the diagenetic evolution process and hydrocarbon accumulation periods of the Chang 8 reservoir in the study area were determined through various techniques. More specifically, core observation, scanning electron microscopy (SEM), X-ray diffraction (XRD), and vitrinite reflectance experiments were performed in combination with systematic studies on rock pyrolysis and the thermal evolutionary history of basins, the illite-dating method, and so on. The Chang 8 reservoir is dominated by feldspar lithic and lithic feldspar sandstones. Quartz, feldspar, and lithic fragments are the major clastic constituents. In clay minerals, the chlorite content is the highest, followed by illite/smectite formation and kaolinite, while the illite content is the lowest. The major diagenesis effect of the Chang 8 reservoir includes compaction, cementation, dissolution, metasomatism, and rupturing. The assumed diagenetic sequence is the following: mechanical composition → early sedimentation of chlorite clay mineral membrane → early cementation of sparry calcite → authigenic kaolinite precipitation → secondary production and amplification of quartz → dissolution of carbonate cement → dissolution of feldspar → late cementation of minerals such as ferrocalcite. Now, the study area is in Stage A in the middle diagenetic period. Through the inclusion of temperature measurements, in conjunction with illite dating and thermal evolutionary history analysis technology in basins, the Chang 8 reservoir of this study was determined as the phase-I continuous accumulation process and the reservoir formation epoch was 105~125 Ma, which was assigned to the Middle Early Cretaceous Epoch.

Increasing evidences have suggested that natural killer (NK) cells in the tumor microenvironment are involved in the regulation of cancer development. However, the potential biological roles and regulatory mechanisms of NK cells in pancreatic cancer (PC) remain unclear. Co-culture system of NK cells with PC cells is used to test the ability of cancer cell proliferation, migration and invasion both in vitro and in vivo. And tail vein intravenous transfer was used to test metastasis in vivo. Meanwhile, extracellular vesicles (EVs) were separated and examined. Furthermore, reporter assay and Biotin-RNA pull down assay were performed to verify the interaction between molecules. NK cells can inhibit the malignant transformation of co-cultured PC cells both in vivo and in vitro, which requires miR-3607-3p. miR-3607-3p is found enriched in the EVs of NK cells and transmitted to PC cells, and low level of miR-3607-3p predicts poor prognosis in PC patients. It can also inhibit proliferation, migration and invasion of PC cells in vitro. Importantly, IL-26 is found to be a direct target of miR-3607-3p in PC cells. miR-3607-3p enriched in EVs derived from NK cells can inhibit the malignant transformation of PC probably through directly targeting of IL-26.

Abstract Background Arsenic trioxide (As2O3), a drug that has been used in China for approximately two thousand years, induces cell death in a variety of cancer cell types, including neuroblastoma (NB). The tyrosine kinase receptor (Trk) family comprises three members, namely TrkA, TrkB and TrkC. Various studies have confirmed that TrkA and TrkC expression is associated with a good prognosis in NB, while TrkB overexpression can lead to tumor cell growth and invasive metastasis. Previous studies have shown that As2O3 can inhibit the growth and proliferation of a human NB cell line and can also affect the N-Myc mRNA expression. It remains unclear whether As2O3 regulates Trks for the purposes of treating NB. Methods The aim of the present study was to investigate the effect of As2O3 on Trk expression in NB cell lines and its potential therapeutic efficacy. SK-N-SH cells were grown with increasing doses of As2O3 at different time points. We cultured SK-N-SH cells, which were treated with increasing doses of As2O3 at different time points. Trk expression in the NB samples was quantified by immunohistochemistry, and the cell cycle was analyzed by flow cytometry. TrkA, TrkB and TrkC mRNA expression was evaluated by real-time PCR analysis. Results Immunohistochemical and real-time PCR analyses indicated that TrkA and TrkC were over-expressed in NB, and specifically during stages 1, 2 and 4S of the disease progression. TrkB expression was increased in stage 3 and 4 NB. As2O3 significantly arrested SK-N-SH cells in the G2/M phase. In addition, TrkA, TrkB and TrkC expression levels were significantly upregulated by higher concentrations of As2O3 treatment, notably in the 48-h treatment period. Our findings suggested that to achieve the maximum effect and appropriate regulation of Trk expression in NB stages 1, 2 and 4S, As2O3 treatment should be at relatively higher concentrations for longer delivery times;however, for NB stages 3 and 4, an appropriate concentration and infusion time for As2O3 must be carefully determined. Conclusion The present findings suggested that As2O3 induced Trk expression in SK-N-SH cells to varying degrees and may be a promising adjuvant to current treatments for NB due to its apoptotic effects.

Spray-formed hypereutectic Al-Si-Cu-Mg alloy is the candidate for automotive and aerospace industries due to its superior wear resistance, lower thermal expansion coefficient and density, and higher thermal conductivity. This paper aims to investigate the bonding properties of hypereutectic Al-25Si-4Cu-1Mg alloys using the transient liquid phase (TLP) method with Cu as an interlayer. To obtain the suitable bonding parameters, the interfacial microstructure and shear strength of Al-25Si-4Cu-1Mg joints were investigated with the effect of different bonding temperatures and holding times. The results showed that TLP bonding between Al-Si-Mg-Cu alloy was mainly realized by large amounts of Al2Cu intermetallic compounds (IMCs), primary Si and α-Al phases. With the brazing temperature increasing, the width of the brazing seam gradually increased, and the voids began to be produced. With the holding time increasing, θ-Al2Cu phases approached into the base metal and Si particles in the brazing seam were obviously coarsened. With the formation of θ-Al2Cu phases into the base metal, more Si particles were segregated at the interface between brazing seam and base metal, and the shear test confirmed that it was the weakest bonding location. Finally, the effect of bonding parameters on the joint strength indicated that the joint brazed at 540 °C for 7.5 min presented the best shear performance with the shear strength reaching 75 MPa because the size of Si particles in the brazing seam was closest to the size of Si particles in base metal under this parameter.

Background: Arsenic trioxide is effective in the treatment of acute promyelocytic leukemia and is currently in use in clinical trials for the treatment of solid tumor types. Given that arsenic trioxide is able to arrest neuroblastoma cell cycle in the G2/M phase, the present study is, to the best of our knowledge, the first to investigate whether the combination of arsenic trioxide with mitosis-phase-specific antineoplastic agents (vinorelbine or docetaxel) or non-mitosis-phase-specific antineoplastic agents (etoposide or cisplatin) exert synergistic effects in cytotoxicity on the human SK-N-SH neuroblastoma cell line.Methods: Neuroblastoma cells were either incubated with one of the four drugs individually, or preincubated with arsenic trioxide and then followed by another drug when cell cycle was arrested at the G2/M phase with the highest proportion.Results: The results of the present study revealed that arsenic trioxide potentiated the apoptotic rate of neuroblastoma cells induced by chemotherapeutic drugs. The present study further demonstrated that preincubation with arsenic trioxide followed by a mitosis-phase-specific antineoplastic agent result in a higher cytotoxicity effect compared with a non mitosis-phase-specific antineoplastic agent. Along with the enhanced cytotoxicity in combination group, the cell cycle distribution demonstrated a decreased proportion of G2/M phase in the combination group.Conclusion: The in vitro study revealed that the pre-application of arsenic trioxide followed by mitosis-phase-specific antineoplastic agents potentiate the cytotoxic effects on neuroblastoma cells, therefore arsenic trioxide may be a promising therapeutic option for treating neuroblastoma.

Prefabricated construction has been widely accepted as an alternative to conventional cast-in-situ construction, given its improved performance. Great efforts have also been made to develop prefabricated construction technologies in China. However, there is a lack of an appropriate pattern for evaluating its comprehensive economic merits, and reasonable mathematical models for providing a comparative analysis of conventional cast-in-situ and prefabricated building projects have yet to be developed. Therefore, the research in this paper aims to comprehensively evaluate the economic benefits of implementing prefabricated construction techniques in order to surpass the economic barrier and promote the development of prefabricated buildings in China. The comprehensive economic evaluation is formulated in terms of resource-use efficiencies, project progress, and incentive policies. An apartment building in Shanghai is selected as a case study. Construction progress is simulated on the BIM platform when the same case study is rationally transformed from the prefabricated to the conventional cast-in-situ construction technique. The results reveal that the comprehensive economic merit can reach ¥739.6/m2 when selecting the prefabricated construction process. The economic benefit brought by shortening the construction period can be regarded as the most significant contributor. Yet, the current incentive policies only contribute 7.1% of the comprehensive economic evaluation. Overall, this research contributes an assessment framework for decision-making in the technique management of building construction. The BIM-based simulation approach can greatly help investors to identify the relevant economic factors and adopt the latest incentive policies.

Atmospheric turbulence threatens flight safety of civil aviation aircraft by inducing aircraft bumpiness. A severity estimation method of aircraft bumpiness in turbulent flight is explored according to in-situ Eddy Dissipation Rate (EDR) indicator. With the turbulence intensity derived from EDR value, a time series of longitudinal and vertical turbulence was generated according to von Karman turbulence model. In order to obtain the vertical acceleration response of aircraft, the continuous change of aerodynamic force on the assembly of wing and horizontal tail was computed by Unsteady Vortex Lattice Method (UVLM). The computing accuracy was improved by using semi-circle division and assigning the vortex rings on the mean camber surface. Furthermore, the adverse effects of control surface deflections on bumpiness severity estimation can be effectively removed by separating turbulence-induced and aircraft maneuvers-induced aerodynamic force change. After that, the variance of vertical acceleration, as the severity indicator of aircraft bumpiness, was obtained by Welch spectrum estimation. With the refined grid level, the pitching moment change due to control surface deflections can be solved accurately by UVLM. The instantaneous acceleration change obtained by UVLM approximates recorded acceleration data with better accuracy than linear transfer function model. A further test with a set of flight data on the same airway shows that compared with in-situ EDR indicator, the proposed method gives an aircraft-dependent estimation of bumpiness severity, which can not only be used to estimate in-situ bumpiness but also be applied to forecast the bumpiness severity of other different aircrafts.