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The study of sexual behavior and the identification of the signals involved in mate recognition between con-specifics are key components that can shed some light, as part of an integrative taxonomic approach, in delimitating species within species complexes. In the Tephritidae family several species complexes have received particular attention as they include important agricultural pests such as the Ceratitis fasciventris (Bezzi), Ceratitis anonae (Graham) and Ceratitis rosa Karsch (FAR) complex, the Bactrocera dorsalis (Hendel) complex and the Anastrepha fraterculus (Wiedemann) complex. Here the value and usefulness of a methodology that uses walk-in field cages with host trees to assess, under semi-natural conditions, mating compatibility within these complexes is reviewed, and the same methodology to study the role of chemical communication in pre-mating isolation among A. fraterculus populations is used. Results showed that under the same experimental conditions it was possible to distinguish an entire range of different outcomes: from full mating compatibility among some populations to complete assortative mating among others. The effectiveness of the methodology in contributing to defining species limits was shown in two species complexes: A. fraterculus and B. dorsalis, and in the case of the latter the synonymization of several established species was published. We conclude that walk-in field cages constitute a powerful tool to measure mating compatibility, which is also useful to determine the role of chemical signals in species recognition. Overall, this experimental approach provides a good source of information about reproductive boundaries to delimit species. However, it needs to be applied as part of an integrative taxonomic approach that simultaneously assesses cytogenetic, molecular, physiological and morphological traits in order to reach more robust species delimitations.

p. 1107-1115 Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-05-08T13:11:53Z No. of bitstreams: 1 024%2E096%2E0351.pdf: 193849 bytes, checksum: 2d1d5e70641c40134c46b3c3978992bf (MD5) Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2014-09-09T14:23:09Z (GMT) No. of bitstreams: 1 024%2E096%2E0351.pdf: 193849 bytes, checksum: 2d1d5e70641c40134c46b3c3978992bf (MD5) Made available in DSpace on 2014-09-09T14:23:09Z (GMT). No. of bitstreams: 1 024%2E096%2E0351.pdf: 193849 bytes, checksum: 2d1d5e70641c40134c46b3c3978992bf (MD5) Previous issue date: 2013 The South American fruit fly Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) cryptic species complex is presently one of the most studied pest models in terms of speciation and population mating compatibility. The improvement of pest-control techniques has strongly relied on successful implementation of laboratory strains into wild populations. Pheromone communication plays an important role in the mating process in the South American fruit fly. Therefore, the main goal of the present study was to investigate the pheromone composition of 7 different populations, originating from geographically distant locations in Brazil and Argentina. Fourteen volatile compounds were identified in calling male emanations by GC×GC/TOF-MS and the data obtained were subsequently analyzed by multivariate statistics. The pheromone composition varied both quantitatively and qualitatively among the studied populations. These results will serve as the basis for further electrophysiological analyses.

Title: Project teaching in art education on primary school - "Meadow" Author: Blanka Kalinová Department: Art Education Department Supervisor: Mgr. Linda Arbanová, Ph.D. Abstract: In this bachelor work the author applies herself to the project teaching of Art lessons. She prioritizes two main educational aims. She chooses a topic and prepares "The Meadow" project. She is succesful at its realization. This theme is enough rich in the impulses for visual work in Art lessons and it infuses well the other subjects. The author makes the project on the base of the School Education Programme for the second year. Her choice of graphic technologies goes with it - drawing, painting, collage, frottage, muchlage, making plastics, objects etc. The whole project consists of eight parts. Each one of them contains a different graphic problem. The description of all graphic activities are completed with the visual documentation, the pupil's reflection and the author's own as well. In the ending of her work she compares the development of children's personality in the class with the growing plants on the meadow. Keywords: Project - meadow - art work - primary school - experiential learning.