30 results on '"Kamma J"'
Search Results
2. Cytokines in gingival crevicular fluid of adolescents and young adults
- Author
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Kamma, J., Mombelli, A., Tsinidou, K., Vasdekis, V., and Giannopoulou, C.
- Published
- 2009
3. Microbiological profile of early onset/aggressive periodontitis patients
- Author
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Kamma, J. J., Nakou, M., Gmür, R., and Baehni, P. C.
- Published
- 2004
4. Microbiological evaluation of dental unit water systems in general dental practice in Europe
- Author
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Walker, J. T., Bradshaw, D. J., Finney, M., Fulford, M. R., Frandsen, E., Østergaard, E., ten Cate, J. M., Moorer, W. R., Schel, A. J., Mavridou, A., Kamma, J. J., Mandilara, G., Stösser, L., Kneist, S., Araujo, R., Contreras, N., Goroncy-Bermes, P., OʼMullane, D., Burke, F., Forde, A., OʼSullivan, M., and Marsh, P. D.
- Published
- 2004
5. The herpesvirus–Porphyromonas gingivalis–periodontitis axis
- Author
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Slots, J., Kamma, J. J., and Sugar, C.
- Published
- 2003
6. Cytomegalovirus periodontal presence is associated with subgingival Dialister pneumosintes and alveolar bone loss
- Author
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Slots, J., Sugar, C., and Kamma, J. J.
- Published
- 2002
7. Profile of subgingival microbiota in children with mixed dentition
- Author
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Kamma, J. J., Diamanti-Kipioti, A., Nakou, M., and Mitsis, F. J.
- Published
- 2000
8. Clinical and microbiological characteristics of smokers with early onset periodontitis
- Author
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Kamma, J. J., Nakou, M., and Baehni, P. C.
- Published
- 1999
9. Detection of gingival crevicular fluid cytokines in children and adolescents with and without fixed orthodontic appliances
- Author
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Giannopoulou, C. Mombelli, A. Tsinidou, K. Vasdekis, V. Kamma, J.
- Abstract
Objective. To study the expression of IL-1β, IL-4, and IL-8 in the gingival crevicular fluid (GCF) of children, adolescents, and young adults with and without fixed orthodontic appliances. Material and methods. Eighty systemically healthy children and adolescents participated in the study: 56 aged between 8 and 16 years without any orthodontic appliance (Group A) and 24 aged between 10 and 20 years having worn fixed orthodontic appliances for at least 12 months (Group B). Clinical examination included presence or absence of plaque, probing depth, bleeding on probing, and gingival overgrowth. GCF was collected by means of Durapore strips from four randomly selected sites per subject. The contents of interleukin-1 beta (IL-1β), interleukin-4 (IL-4), and interleukin-8 (IL-8) were detected by ELISA, measured as total amounts (pg/30s) and expressed in log scale. Results. Statistically significant differences were noted for the mean log IL-1β, IL-4, and IL-8 between the two groups: Group B showed significantly higher mean levels in log IL-1β and log IL-8 compared to Group A. Mean levels of log IL-4 were lower in Group B, although they did not reach statistical significance. Furthermore, mean levels of log IL-1β and log IL-8 were associated with bleeding sites (p
- Published
- 2008
10. Attitudes of general dental practitioners in Europe to the microbial risk associated with dental unit water systems
- Author
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Kamma, J. J., Bradshaw, D. J., Fulford, M. R., Marsh, P. D., Frandsen, E., Østergaard, E., Schel, A. J., Ten Cate, J. M., Moorer, W. R., Mavridou, A., Mandilara, G., Stoesser, L., Kneist, S., Araujo, R., Contreras, N., Goroncy-Bermes, P., Burke, F., O Mullane, D., Maurice O'Sullivan, Walker, J. T., Cariologie/EPT (OUD, ACTA), and Medical Microbiology and Infection Prevention
- Abstract
Dental Unit Water Systems (DUWS) are used in dental practices to provide water for cooling of dental equipment and irrigation of the oral cavity. However, they have been demonstrated to be contaminated with micro-organisms. There are currently no European Union (EU) Commission guidelines for the microbial quality of water discharged by DUWS. This study was part of an EU research programme to investigate the microbial contamination of DUWS in general dental practice (GDP) in the UK, Denmark, Germany, The Netherlands, Ireland, Greece and Spain. To undertake a questionnaire survey on the type of DUWS in use and determine the attitude of GDPs to the risk of microbial infection from DUWS. The questionnaire was written and translated into the language of each country before being posted to each participating dentist. Dentists were asked to complete the questionnaire survey and return it by post. The major findings were that the majority of dentists did not clean, disinfect or determine the microbial load of their DUWS, and that dentists would welcome regular monitoring and advice on maintaining their DUWS; the introduction of guidelines; and recommendations on controlling the microbial load of DUWS
- Published
- 2006
11. Periodontal microflora of HIV infected patients with periodontitis
- Author
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Nakou, M Kamma, J Gargalianos, P Laskaris, G Mitsis, F
- Abstract
The aim of this study was to determine the microbial profile of periodontal lesions in HIV seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. The subgingival microflora of 20 CDC II, 20 CDC III, 20 CDC IV/V and 20 systemically healthy patients with rapidly progressing periodontitis was examined. Four sites with greatest probing depth in each patient were selected for microbiological sampling. The samples were cultured aerobically and anaerobically for bacterial isolation using selective and nonselective media. Isolates were characterized to species level by conventional biochemical tests and various identification kits. The microflora of periodontitis lesions within the three stages of the HN infection was similar to that of progressing periodontitis in systemically healthy adults including Campylobacter rectus, Capnocytophaga spp., Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, Selenomonas spp. and Peptostreptococcus micros. However, HN seropositive periodontitis lesions harboured a range of exogenous pathogens rarely associated with common types of periodontitis including Staphylococcus aureus, Enterobacter cloaca, Pseudomonas aeruginosa, Candida albicans, Enterococcus faecalis, Enterococcus avium, Clostridium difficile, Aspergillus fumigatus, Klebsiella pneumoniae and Mycoplasma incognitum. The lack of immune effector and regulatory cells in HIV infected patients could in fact explain the increase of some opportunistic pathogens and the characteristic and rapidly progressing nature of the periodontal disease in these patients. (C) 1997 Academic Press.
- Published
- 1997
12. Association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.
- Author
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Kamma, J J, Nakou, M, Persson, G. Rutger, Kamma, J J, Nakou, M, and Persson, G. Rutger
- Abstract
OBJECTIVES: The objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (AST) in gingival crevicular fluid (GCF) using the colorimetric PerioGard (PTM) test and the subgingival microflora in early onset periodontitis lesions. MATERIAL AND METHODS: The study population consisted of 25 otherwise healthy individuals exhibiting early onset periodontitis (EOP). In each patient four experimental sites were identified comprising one deep periodontal pocket (PD >5 mm) randomly chosen in each quadrant. Bacterial samples were obtained from the experimental sites, consecutively cultured anaerobically and in 10% CO(2) using selective and nonselective media. Isolates were characterized to species level by conventional biochemical tests and various identification kits. Clinical measurements as well as AST activity, assessed either as positive or negative using the PTM, were recorded at the same sites. RESULTS: Sixty-two sites exhibited AST positive and 38 AST negative activity. Analysis of bacterial counts using the ANOVA (Mann Whitney U-test) showed that Streptococcus intermedius, Peptostreptococcus micros, Campylobacter concisus, Bacteroides forsythus, Camplobacter gracilis, Campylobacter rectus and Selenomonas sputigena were significantly higher in sites with AST-positive activity. The odds ratio of having high prevalence of S. intermedius, P. micros, C. concisus, B. forsythus, C. gracilis, C. rectus and S. sputigena in the presence of a positive AST site was very high (range: 3.5-17.0). Streptococcus sanguis, Actinomyces naeslundii, Gemella morbillorum, Capnocytophaga gingivalis, Veillonella parvula, Fusobacterium varium, Eubacterium lentum and Prevotella oralis were detected in significantly higher proportions in sites with AST negative activity and manifested a negative odds ratio in the presence of AST positive sites. The logistic regression analysis revealed that smoking and bleeding upon probing showed a
- Published
- 2001
- Full Text
- View/download PDF
13. Microbial risk assessment of dental unit water systems in general dental practice in Greece
- Author
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Mavridou, A., primary, Kamma, J., primary, Mandilara, G., primary, Delaportas, P., primary, and Komioti, F., primary
- Published
- 2006
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14. Comparison of the Efficacies of Disinfectants To Control Microbial Contamination in Dental Unit Water Systems in General Dental Practices across the European Union
- Author
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Schel, A. J., primary, Marsh, P. D., additional, Bradshaw, D. J., additional, Finney, M., additional, Fulford, M. R., additional, Frandsen, E., additional, Østergaard, E., additional, ten Cate, J. M., additional, Moorer, W. R., additional, Mavridou, A., additional, Kamma, J. J., additional, Mandilara, G., additional, Stösser, L., additional, Kneist, S., additional, Araujo, R., additional, Contreras, N., additional, Goroncy-Bermes, P., additional, O'Mullane, D., additional, Burke, F., additional, O'Reilly, P., additional, Hourigan, G., additional, O'Sullivan, M., additional, Holman, R., additional, and Walker, J. T., additional
- Published
- 2006
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15. Periodontal Microflora of HIV Infected Patients with Periodontitis
- Author
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Nakou, M, primary, Kamma, J, additional, Gargalianos, P, additional, Laskaris, G, additional, and Mitsis, F, additional
- Published
- 1997
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16. Microbiological profile of early onset/aggressive periodontitis patients
- Author
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P. Baehni, M Nakou, Rudolf Gmür, Joanna J. Kamma, University of Zurich, and Kamma, J J
- Subjects
Adult ,Male ,Microbiology (medical) ,Immunology ,Population ,Alveolar Bone Loss ,Colony Count, Microbial ,Dental Plaque ,610 Medicine & health ,Sensitivity and Specificity ,Microbiology ,2726 Microbiology (medical) ,Bacteria, Anaerobic ,Prevotella nigrescens ,Forsythia ,medicine ,Humans ,Aggressive periodontitis ,Tannerella forsythia ,Fluorescent Antibody Technique, Indirect ,education ,General Dentistry ,Porphyromonas gingivalis ,Ecosystem ,2403 Immunology ,education.field_of_study ,biology ,2404 Microbiology ,Prevotella intermedia ,Campylobacter rectus ,biology.organism_classification ,medicine.disease ,3500 General Dentistry ,Bacterial Typing Techniques ,Radiography ,10182 Institute of Oral Biology ,stomatognathic diseases ,Aggressive Periodontitis ,Acute Disease ,Female - Abstract
OBJECTIVES: The objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence. MATERIAL AND METHODS: The study group consisted of 66 systemically healthy individuals with evidence of early onset periodontitis - 41 females and 25 males aged 23-35 years (mean 31.1 +/- 3.1 years). Bacterial samples were collected from the deepest site in each quadrant, resulting in a total of 264 sites with a mean probing pocket depth of 6.6 +/- 1.5 mm. Samples were cultured anaerobically and in 10% CO(2) using selective and nonselective media, and isolates were characterized to species level. Indirect immunofluorescence using monoclonal antibodies was applied to detect Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia (Bacteroides forsythus, Tannerella forsythensis), Prevotella intermedia/Prevotella nigrescens, Campylobacter rectus, Peptostreptococcus micros and Actinomyces israelii. RESULTS: 93.6% of sampled sites showed bleeding on probing and 23.5% were positive for suppuration. P. intermedia/P. nigrescens, P. gingivalis, and C. rectus were detected in 77.3-85.9% of samples using culture methods and in 85.6-91.3% using immunofluorescence. P. micros and A. actinomycetemcomitans were found, respectively, in 63.3% and 25.0% of all sites using culturing and in 58.7% and 27.7% sites using immunofluorescence. Significantly strong positive associations were observed between T. forsythia and C. rectus (odds ratio 109.46), and T. forsythia and P. gingivalis (odd ratio 90.26), whereas a negative association was seen between P. intermedia/P. nigrescens and A. actinomycetemcomitans (odds ratio 0.42). Coinfection by P. gingivalis, T. forsythia, P. intermedia/P. nigrescens and C. rectus was observed in 62.1% of the test sites, and in 89.4% of the studied subjects. The sensitivity of immunofluorescence for T. forsythia, C. rectus, P. intermedia/P. nigrescens and P. gingivalis was found to be very high (0.99-0.94) using culture as the reference detection method. The agreement between culture and immunofluorescence in detecting the presence or absence of the investigated species was 85.2-88.1% for P. gingivalis, P. intermedia/P. nigrescens, C. rectus, and T. forsythia, 75.9% for A. actinomycetemcomitans and 70.4% for P. micros. CONCLUSIONS: The microbial profile of the early onset/aggressive periodontitis population was complex. The agreement between the two detection methods was very high.
- Published
- 2004
17. European Federation of Periodontology-the first twenty-five years: periodontal health for a better life.
- Author
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Wilson NHF, Kamma J, and Chapple ILC
- Subjects
- Europe, Humans, Periodontium, Oral Health, Periodontal Diseases prevention & control, Periodontics organization & administration, Societies, Dental organization & administration
- Abstract
The European Federation of Periodontology (EFP), founded in 1991, has become one of Europe's foremost dental organisations and a global force in the field of Periodontology, including implant dentistry. This paper records the many, different achievements of the EFP in its short but selfless and highly successful contributions to the Federation.
- Published
- 2017
18. Detection of gingival crevicular fluid cytokines in children and adolescents with and without fixed orthodontic appliances.
- Author
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Giannopoulou C, Mombelli A, Tsinidou K, Vasdekis V, and Kamma J
- Subjects
- Adolescent, Adult, Child, Female, Gingival Crevicular Fluid immunology, Gingival Hyperplasia immunology, Gingival Hyperplasia metabolism, Humans, Interleukin-1beta metabolism, Interleukin-4 metabolism, Interleukin-8 metabolism, Male, Gingival Crevicular Fluid metabolism, Interleukins metabolism, Orthodontic Appliances, Periodontal Index
- Abstract
Objective: To study the expression of IL-1beta, IL-4, and IL-8 in the gingival crevicular fluid (GCF) of children, adolescents, and young adults with and without fixed orthodontic appliances., Material and Methods: Eighty systemically healthy children and adolescents participated in the study: 56 aged between 8 and 16 years without any orthodontic appliance (Group A) and 24 aged between 10 and 20 years having worn fixed orthodontic appliances for at least 12 months (Group B). Clinical examination included presence or absence of plaque, probing depth, bleeding on probing, and gingival overgrowth. GCF was collected by means of Durapore strips from four randomly selected sites per subject. The contents of interleukin-1 beta (IL-1beta), interleukin-4 (IL-4), and interleukin-8 (IL-8) were detected by ELISA, measured as total amounts (pg/30s) and expressed in log scale., Results: Statistically significant differences were noted for the mean log IL-1beta, IL-4, and IL-8 between the two groups: Group B showed significantly higher mean levels in log IL-1beta and log IL-8 compared to Group A. Mean levels of log IL-4 were lower in Group B, although they did not reach statistical significance. Furthermore, mean levels of log IL-1beta and log IL-8 were associated with bleeding sites (p<0.001) and gingival overgrowth, while mean level of log IL-4 was associated with non-bleeding sites and no gingival overgrowth (p<0.001)., Conclusion: Our findings suggest that fixed orthodontic appliances result in an increase in the expression of IL-1beta and IL-8. This may reflect biologic activity in the periodontium during orthodontic tooth movement.
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- 2008
- Full Text
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19. Attitudes of general dental practitioners in Europe to the microbial risk associated with dental unit water systems.
- Author
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Kamma JJ, Bradshaw DJ, Fulford MR, Marsh PD, Frandsen E, Ostergaard E, Schel AJ, ten Cate JM, Moorer WR, Mavridou A, Mandilara G, Stoesser L, Kneist S, Araujo R, Contreras N, Goroncy-Bermes P, Burke F, O'Mullane D, O'Sullivan M, and Walker JT
- Subjects
- Europe, Humans, Surveys and Questionnaires, Water Microbiology standards, Attitude of Health Personnel, Dental Equipment microbiology, Infection Control, Dental methods, Water Supply standards
- Abstract
Unlabelled: Dental Unit Water Systems (DUWS) are used in dental practices to provide water for cooling of dental equipment and irrigation of the oral cavity. However, they have been demonstrated to be contaminated with micro-organisms. There are currently no European Union (EU) Commission guidelines for the microbial quality of water discharged by DUWS. This study was part of an EU research programme to investigate the microbial contamination of DUWS in general dental practice (GDP) in the UK, Denmark, Germany, The Netherlands, Ireland, Greece and Spain., Objective: To undertake a questionnaire survey on the type of DUWS in use and determine the attitude of GDPs to the risk of microbial infection from DUWS., Materials and Methods: The questionnaire was written and translated into the language of each country before being posted to each participating dentist. Dentists were asked to complete the questionnaire survey and return it by post., Results and Conclusions: The major findings were that the majority of dentists did not clean, disinfect or determine the microbial load of their DUWS, and that dentists would welcome regular monitoring and advice on maintaining their DUWS; the introduction of guidelines; and recommendations on controlling the microbial load of DUWS.
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- 2006
- Full Text
- View/download PDF
20. General health risk of periodontal disease.
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Slots J and Kamma JJ
- Subjects
- Aggregatibacter actinomycetemcomitans physiology, Cardiovascular Diseases etiology, Chronic Disease, Disease Susceptibility, Female, Focal Infection, Dental microbiology, Humans, Infant, Low Birth Weight, Infant, Newborn, Periodontal Diseases microbiology, Porphyromonas gingivalis physiology, Pregnancy, Pregnancy Complications, Risk Factors, Stroke etiology, Disease etiology, Periodontal Diseases complications
- Abstract
The possibility that periodontal disease might influence the morbidity and mortality of systemic diseases constitutes a research topic of great current interest. Human periodontal disease is associated with a complex microbiota containing approximately 500 microbial taxa and various human viruses, many of which possess significant virulence potential. Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and other periodontopathic bacteria that are unique to the oral cavity and may disseminate to other body sites comprise the best-documented form of dental focal infection. However, systemically healthy individuals seem to be at low risk of acquiring acute non-oral diseases from direct infections by periodontal pathogens. Research data from various laboratories point to periodontal infections as a risk factor for chronic medical disorders, including cardiovascular disease, cerebrovascular accidents and low-birth-weight infants. However, recent epidemiological studies have failed to show a significant relationship between periodontal disease and cardiovascular disease. This review paper evaluates the current status of knowledge on dental focal infection and suggests avenues for further research into the topic of general health risks of periodontal disease.
- Published
- 2001
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21. Association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.
- Author
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Kamma JJ, Nakou M, and Persson RG
- Subjects
- Adult, Analysis of Variance, Bacteria, Anaerobic isolation & purification, Bacteria, Anaerobic pathogenicity, Colony Count, Microbial, Cross-Sectional Studies, Dental Plaque microbiology, Female, Humans, Logistic Models, Male, Odds Ratio, Aggressive Periodontitis enzymology, Aggressive Periodontitis microbiology, Aspartate Aminotransferases metabolism, Gingival Crevicular Fluid enzymology, Gingival Crevicular Fluid microbiology
- Abstract
Objectives: The objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (AST) in gingival crevicular fluid (GCF) using the colorimetric PerioGard (PTM) test and the subgingival microflora in early onset periodontitis lesions., Material and Methods: The study population consisted of 25 otherwise healthy individuals exhibiting early onset periodontitis (EOP). In each patient four experimental sites were identified comprising one deep periodontal pocket (PD >5 mm) randomly chosen in each quadrant. Bacterial samples were obtained from the experimental sites, consecutively cultured anaerobically and in 10% CO(2) using selective and nonselective media. Isolates were characterized to species level by conventional biochemical tests and various identification kits. Clinical measurements as well as AST activity, assessed either as positive or negative using the PTM, were recorded at the same sites., Results: Sixty-two sites exhibited AST positive and 38 AST negative activity. Analysis of bacterial counts using the ANOVA (Mann Whitney U-test) showed that Streptococcus intermedius, Peptostreptococcus micros, Campylobacter concisus, Bacteroides forsythus, Camplobacter gracilis, Campylobacter rectus and Selenomonas sputigena were significantly higher in sites with AST-positive activity. The odds ratio of having high prevalence of S. intermedius, P. micros, C. concisus, B. forsythus, C. gracilis, C. rectus and S. sputigena in the presence of a positive AST site was very high (range: 3.5-17.0). Streptococcus sanguis, Actinomyces naeslundii, Gemella morbillorum, Capnocytophaga gingivalis, Veillonella parvula, Fusobacterium varium, Eubacterium lentum and Prevotella oralis were detected in significantly higher proportions in sites with AST negative activity and manifested a negative odds ratio in the presence of AST positive sites. The logistic regression analysis revealed that smoking and bleeding upon probing showed a significant association with AST activity, while plaque and suppuration were not found to be significant predictors of AST activity. The co-infection of Porphyromonas gingivalis, B. forsythus and P. micros, or P. gingivalis, B. forsythus and C. rectus were found to be significantly associated with the AST activity (p<0.001). AST positive sites revealed significantly higher occurrence of co-infections by P. gingivalis, B. forsythus, S. sputigena or by P. gingivalis, B. forsythus, S. intermedius than AST negative sites (p<0.001). P. gingivalis, B. forsythus, A. naeslundii co-infection was found significantly higher in the AST negative sites (p<0.001)., Conclusions: The present study found a high level of agreement between the presence of putative periodontal pathogens and positive AST scores at periodontal sites that clinically were considered to be potentially disease active. Prospective studies should be performed to confirm the findings.
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- 2001
- Full Text
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22. Herpes viruses and periodontopathic bacteria in early-onset periodontitis.
- Author
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Kamma JJ, Contreras A, and Slots J
- Subjects
- Adult, Aggregatibacter actinomycetemcomitans isolation & purification, Bacteria pathogenicity, Bacteroides isolation & purification, Chronic Disease, Cytomegalovirus isolation & purification, Female, Herpesvirus 4, Human isolation & purification, Humans, Male, Periodontal Index, Polymerase Chain Reaction, Porphyromonas gingivalis isolation & purification, Simplexvirus isolation & purification, Bacteria isolation & purification, Herpesviridae immunology, Herpesviridae isolation & purification, Immune Tolerance, Periodontitis microbiology, Periodontitis virology
- Abstract
Objectives: This study examined the occurrence of human herpes viruses and suspected periodontopathic bacteria in early-onset periodontitis patients who experienced progressive disease in at least 2 periodontal sites during the maintenance phase of therapy., Material and Methods: In each of 16 individuals (9 male and 7 female; mean age 33.1+/-2.6 years), subgingival plaque samples were collected from 2 deteriorating and 2 stable periodontitis sites. A nested polymerase chain reaction method determined the presence of human cytomegalovirus (HCMV), Epstein-Barr virus type 1 (EBV-1) and herpes simplex virus (HSV). A 16s rRNA polymerase chain reaction method identified Porphyromonas gingivalis, Dialister pneumosintes, Bacteroides forsythus and Actinobacillus actinomycetemcomitans., Results: HCMV was detected in 59.4% of active and in 12.5% of stable sites (p<0.001), EBV-1 in 43.8% of active and in 12.5 % of stable sites (p=0.01), HSV in 34.5% of active and in 9.4% of stable sites (p=0.03), and co-infection with any of the 3 test herpesviruses in 43.8% of active and in 3.1% of stable sites (p<0.001). P. gingivalis was detected in 71.9% of active and in 37.5% of stable sites (p=0.01), D. pneumosintes in 62.5% of active and in 18.8% of stable sites (p=0.04), co-infection with P. gingivalis and D. pneumosintes in 50% of active and in 0% of stable sites (p<0.001), and co-infection with any 3 or 4 of the test bacteria in 40.6% of active and in 0% of stable sites (p=0.001). All periodontitis sites showing herpesvirus co-infection and all but one site showing P. gingivalis and D. pneumosintes co-infection revealed bleeding upon probing., Conclusions: HCMV, EBV-1, HSV and herpesvirus co-infection, as well as P. gingivalis, D. pneumosintes and P. gingivalis-D. pneumosintes co-infection were statistically associated with active periodontitis. Herpesviruses are immunosuppressive and may set the stage for overgrowth of subgingival P. gingivalis, D. pneumosintes and other periodontopathic bacteria. Understanding the significance of herpesviruses in human periodontitis may allow for improved diagnosis, more specific therapy and, ultimately, disease prevention.
- Published
- 2001
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23. Gingival overgrowth as the initial paraneoplastic manifestation of Hodgkin's lymphoma in a child. A case report.
- Author
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Nicolatou-Galitis O, Papadaki T, Moschovi M, Kamma JJ, van Vliet-Constantinidou C, Tsoumakas C, Kattamis A, and Tzortzatou-Stathopoulou F
- Subjects
- Alveolar Bone Loss etiology, Antineoplastic Combined Chemotherapy Protocols administration & dosage, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Biopsy, Bleomycin administration & dosage, Child, Dacarbazine administration & dosage, Doxorubicin administration & dosage, Gingival Hyperplasia etiology, Humans, Lymph Nodes pathology, Male, Mechlorethamine administration & dosage, Neoplasm Staging, Prednisone administration & dosage, Procarbazine administration & dosage, Remission Induction, Root Resorption etiology, Tooth, Deciduous pathology, Vinblastine administration & dosage, Vincristine administration & dosage, Gingival Overgrowth etiology, Hodgkin Disease complications, Paraneoplastic Syndromes etiology
- Abstract
Background: The purpose of this paper is to present the first case of gingival overgrowth, premature root resorption, and alveolar bone loss, which preceded the diagnosis of a stage IVB Hodgkin's lymphoma (HL) in a 9-year-old boy., Methods: The child presented complaining of gingival pain which first appeared 3 months prior. Clinical examination revealed inflamed, hyperplastic gingivae, while x-ray showed premature root resorption and alveolar bone loss. Medical work-up was significant for cervical lymphadenopathy. Gingival biopsy, followed by lymph node resection, was performed twice., Results: Histological examination of both gingival biopsies disclosed a mixed inflammatory infiltrate, while classical Hodgkin's lymphoma of the nodular sclerosis type was diagnosed from the second lymph node biopsy. Chemotherapy was instituted with mustard-vincristine-procarbazine-prednizone and adriamycine-bleomycine-vinblastine-dacarbazine. Remission of the lymphoma was observed with concomitant regression of the gingival overgrowth., Conclusions: The inflammatory gingival overgrowth, premature root resorption of deciduous teeth, and alveolar bone loss in this case, in conjunction with the regression of gingival overgrowth which followed the completion of chemotherapy, are strongly indicative of a paraneoplastic manifestation of HL. The postulated mechanism for the development of the manifestation is the constitutive activation of the transcription factor NF-kB. The gingival inflammatory reaction was probably further aggravated by the bacterial-stimulated cytokine secretion released by monocytes.
- Published
- 2001
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24. The clinical and microbiological effects of systemic ornidazole in sites with and without subgingival debridement in early-onset periodontitis patients.
- Author
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Kamma JJ, Nakou M, and Mitsis FJ
- Subjects
- Administration, Oral, Adult, Aggregatibacter actinomycetemcomitans drug effects, Aggregatibacter actinomycetemcomitans growth & development, Aggressive Periodontitis microbiology, Aggressive Periodontitis therapy, Anti-Bacterial Agents administration & dosage, Bacteroides drug effects, Bacteroides growth & development, Campylobacter drug effects, Campylobacter growth & development, Combined Modality Therapy, Dental Scaling, Female, Follow-Up Studies, Gingival Hemorrhage drug therapy, Gingival Hemorrhage therapy, Humans, Male, Oral Hygiene, Ornidazole administration & dosage, Periodontal Pocket drug therapy, Periodontal Pocket therapy, Porphyromonas gingivalis drug effects, Porphyromonas gingivalis growth & development, Prevotella drug effects, Prevotella growth & development, Prevotella intermedia drug effects, Prevotella intermedia growth & development, Root Planing, Selenomonas drug effects, Selenomonas growth & development, Statistics, Nonparametric, Streptococcus drug effects, Streptococcus growth & development, Streptococcus oralis drug effects, Streptococcus oralis growth & development, Streptococcus sanguis drug effects, Streptococcus sanguis growth & development, Aggressive Periodontitis drug therapy, Anti-Bacterial Agents therapeutic use, Ornidazole therapeutic use, Subgingival Curettage
- Abstract
Background: The purpose of this study was to evaluate the clinical and microbiological effects of systemic ornidazole (ORN) in sites with or without subgingival debridement in early-onset periodontitis (EOP) patients., Methods: Two pooled bacterial samples consisting of 4 sites each (scaled and non-scaled sites) were obtained from 30 individuals exhibiting EOP. All patients received oral hygiene instruction (OHI), supragingival scaling and ORN. Subgingival scaling and root planing (SRP) was carried out only in scaled sites. Bacterial samples were taken at baseline (BL) and 1 week and 2, 6, and 12 months after systemic ornidazole administration (500 mg/bid for 7 days). One more sample was taken at scaled sites, one week after SRP., Results: One week following SRP (scaled sites) Gram-negative facultative and anaerobic rods were significantly reduced while Gram-positive facultative cocci were significantly increased. After ORN administration, P. gingivalis, P. denticola, P. intermedia, B. forsythus, C. rectus, and S. sputigena were no longer detectable in either scaled or non-scaled sites. A statistically significant long-term (2, 6, and 12 months) reduction of P. gingivalis, P. intermedia, P. loescheii, B. forsythus, and C. rectus and a pronounced increase of S. milleri, S. oralis, and S. sanguis counts in both scaled and non-scaled sites were detected in comparison to baseline. A sustained reduction of bleeding tendency and of probing depth was also observed in both scaled and non-scaled sites., Conclusions: ORN combined with SRP effects beneficial shifts in the bacterial population associated with substantial clinical improvement, thereby indicating that ORN is effective adjunct in the treatment of EOP deep periodontal pockets where anaerobic bacteria are predominant.
- Published
- 2000
- Full Text
- View/download PDF
25. Profile of subgingival microbiota in children with primary dentition.
- Author
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Kamma JJ, Diamanti-Kipioti A, Nakou M, and Mitsis FJ
- Subjects
- Actinomyces isolation & purification, Analysis of Variance, Bacteria, Anaerobic isolation & purification, Child, Preschool, Cluster Analysis, Colony Count, Microbial, Dental Plaque Index, Female, Humans, Likelihood Functions, Male, Periodontal Index, Sampling Studies, Streptococcus sanguis isolation & purification, Dental Plaque microbiology, Tooth, Deciduous
- Abstract
Eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. The aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. Subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). The samples were cultured for bacterial isolation anaerobically and in 10% CO2 plus air using selective and non-selective media. Forty-one different microbial species were isolated. Gemella morbillorum and Peptostreptococcus magnus were statistically significantly more frequently detected in incisors while P. micros, Streptococcus intermedius, Bacteroides forsythus, Fusobacterium nucleatum, Prevotella loeschei, P. melaninogenica and Selenomonas sputigena were more frequently detected in molars. The bacterial species S. constellatus, G. morbillorum and P. magnus were isolated in greater numbers in incisors and P. micros, S. intermedius, Campylobacter concisus, Bacteroides egertheii, B. forsythus, P. oralis and S. sputigena were isolated in greater numbers in molars, respectively. Cluster analysis revealed 4 clusters in which 6-7 bacterial species were elevated above mean levels. Cluster I was predominated by S. constellatus, S. mitis, S. sanguis, G. morbillorum, P. melaninogenica and P. oralis; cluster II was predominated by S. sanguis, Actinomyces naeslundii, Capnocytophaga gingivalis, C. ochracea and P. intermedia; cluster III was predominated by S. mitis, C. ochracea, F. nucleatum, P. loeschei, P. melaninogenica and P. oralis; and finally cluster IV was predominated by S. sanguis, C. gingivalis, Veillonella parvula, Campylobacter gracilis, F. nucleatum and P. intermedia. The bacterial species S. constellatus, P. micros, Pseudoramibacter alactolyticus, Eikenella corrodens and F. nucleatum were associated with non-bleeding sites while S. intermedius, C. concisus, P. intermedia and P. loescheii were found more frequently in bleeding sites.
- Published
- 2000
- Full Text
- View/download PDF
26. Subgingival microflora and treatment in prepubertal periodontitis associated with chronic idiopathic neutropenia.
- Author
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Kamma JJ, Lygidakis NA, and Nakou M
- Subjects
- Aggregatibacter actinomycetemcomitans drug effects, Aggregatibacter actinomycetemcomitans isolation & purification, Aggressive Periodontitis complications, Aggressive Periodontitis therapy, Amoxicillin-Potassium Clavulanate Combination therapeutic use, Anti-Bacterial Agents therapeutic use, Capnocytophaga drug effects, Capnocytophaga isolation & purification, Child, Chronic Disease, Dental Plaque microbiology, Dental Scaling, Drug Therapy, Combination therapeutic use, Female, Follow-Up Studies, Humans, Metronidazole therapeutic use, Porphyromonas gingivalis drug effects, Porphyromonas gingivalis isolation & purification, Prevotella intermedia drug effects, Prevotella intermedia isolation & purification, Root Planing, Aggressive Periodontitis microbiology, Bacteria classification, Gingiva microbiology, Neutropenia complications
- Abstract
Prepubertal periodontitis affects both primary and permanent dentition. The purpose of this study was to examine the composition of subgingival microflora of the permanent dentition in an 11-year-old Caucasian female, who had premature exfoliation of her deciduous teeth on her 5th year of age, and the response of this condition to the antibiotic therapy and supportive periodontal care. Gingival tissues were highly inflamed and alveolar bone loss was detected radiographically. The girl had experienced frequent upper respiratory tract infections, tonsilitis and recurrent otitis media. Her mother had history of early onset periodontitis associated with chronic idiopathic neutropenia. Blood chemistry tests and immunological examinations were also performed. Subgingival plaque samples were collected from the proximal sites of permanent molars, incisors, canines and maxillary premolars. 27 different microbial species were isolated from the subgingival microflora. Among the predominant species were Porphyromonas gingivalis (17.6%-7.3%), Prevotella intermedia (12.4%-4.7%), Capnocytophaga sputigena (14.4%-10.4%), Capnocytophaga ochracea (13.2%-6.9%) and Actinobacillus actinomycetemcomitans (9.3%-5.5%). Periodontal treatment consisted of scaling, root planing in conjunction with antibiotic administration of Augmentin 312.5 mg and Flagyl 200 mg, each t.i.d. for 10 days. 3 weeks after the antibiotic therapy, bacterial samples were collected from the same sites. All the periodontal pathogens were recovered in lower levels and A.actinomycetemcomitans was almost eliminated in the 3-week period. The evaluation of clinical indices at 3, 6 and 12 months showed that periodontal treatment in conjunction with antibiotics was effective and rapidly followed by marked clinical improvement. The microbiological monitoring at 3, 6 and 12 months after antibiotic treatment and each time prior to supportive periodontal care, revealed that the periodontal pathogens fluctuated in low levels even 12 months after treatment and could be maintained at low level by supportive periodontal care at 3-month intervals.
- Published
- 1998
- Full Text
- View/download PDF
27. Subgingival microflora associated with nifedipine-induced gingival overgrowth.
- Author
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Nakou M, Kamma JJ, Andronikaki A, and Mitsis F
- Subjects
- Adult, Age Factors, Aged, Bacteroidaceae classification, Bacteroidaceae isolation & purification, Bacteroides isolation & purification, Calcium Channel Blockers administration & dosage, Campylobacter isolation & purification, Capnocytophaga classification, Capnocytophaga isolation & purification, Dental Plaque Index, Eubacterium isolation & purification, Female, Forecasting, Fusobacterium classification, Fusobacterium isolation & purification, Fusobacterium nucleatum isolation & purification, Gingival Hemorrhage pathology, Gingival Overgrowth microbiology, Gingival Overgrowth pathology, Gingival Pocket pathology, Gingivitis chemically induced, Humans, Logistic Models, Male, Middle Aged, Nifedipine administration & dosage, Periodontal Index, Propionibacterium acnes isolation & purification, Calcium Channel Blockers adverse effects, Dental Plaque microbiology, Gingiva microbiology, Gingival Overgrowth chemically induced, Gram-Negative Bacteria classification, Gram-Positive Bacteria classification, Nifedipine adverse effects
- Abstract
The purpose of this study was to examine the composition of subgingival plaque of 140 periodontal lesions in 35 patients with cardiovascular disorders who were administered nifedipine and manifested nifedipine-induced gingival overgrowth (GO). Age was inversely associated with the GO. Plaque index and bleeding index showed a significant association with GO, while nifedipine dosage and duration of nifedipine therapy were not found to be significant predictors of GO. The gingival inflammation as expressed in the logistic regression model by the interaction term color x tone was found to be significantly associated with the GO. Statistically significant differences between the groups of comparable probing depth and different degrees of GO were detected for Propionibacterium acnes, Capnocytophaga gingivalis, Capnocytophaga ochracea, Capnocytophaga sputigena, Bacteroides gracilis, Fusobacterium mortiferum, Fusobacterium nucleatum, Fusobacterium varium and Selenomonas sputigena in deep and enlarged lesions. Significantly more frequently isolated were the bacterial species Eubacterium alactolyticum, Campylobacter concisus, C. gingivalis, C. ochracea, C. sputigena, F. mortiferum, F. nucleatum, and F. varium from the more enlarged lesions (GO >3).
- Published
- 1998
- Full Text
- View/download PDF
28. Subgingival microflora in smokers with early onset periodontitis.
- Author
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Kamma JJ and Nakou M
- Abstract
Cigarette smoking is a potent risk factor which has recently been associated with periodontal disease progression. The objective of this study was to detect the microbial profile of early onset periodontitis in smokers and compare it to that of non-smokers. The study population consisted of 50 systemically healthy individuals aged 25 to 38 years, exhibiting early onset periodontitis. 25 patients were smokers (> 20 cigarettes/day) and 25 non-smokers. Two pooled bacterial samples comprised of four periodontal sites with probing depth > 5 mm each, were collected from each individual. The samples were cultured aerobically and anaerobically for bacterial isolation using selective and non-selective media. Isolates were characterized to species level by conventional biochemical tests and various identification kits. The differences in bacterial counts using the Mann Whitney U test were statistically significant for Staphylococcus aureus, Campylobacter concisus, Eikenella corrodens, Escherichia coli, Bacteroides forsythus, Bacteroides gracilis, Campylobacter rectus, Porphyromonas gingivalis, Selenomonas sputigena and Candida albicans in smokers. Statistically significant differences for Peptostreptococcus micros, Actinomyces naeslundii, Eubacterium lentum and Capnocytophaga gingivalis were detected in non-smokers. The isolation of bacteria belonging to the exogenous flora like E. coli, C. albicans and S. aureus in smokers microflora underscores the importance of the host which is adversely affected by cigarette smoking.
- Published
- 1997
- Full Text
- View/download PDF
29. Predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis.
- Author
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Kamma JJ, Nakou M, and Manti FA
- Subjects
- Actinomyces isolation & purification, Adult, Aggregatibacter actinomycetemcomitans isolation & purification, Alveolar Bone Loss microbiology, Alveolar Bone Loss pathology, Bacteroides isolation & purification, Campylobacter isolation & purification, Dental Plaque microbiology, Female, Fusobacterium nucleatum isolation & purification, Haemophilus isolation & purification, Humans, Male, Periodontal Attachment Loss microbiology, Periodontal Attachment Loss pathology, Periodontitis pathology, Porphyromonas gingivalis isolation & purification, Streptococcus isolation & purification, Veillonella isolation & purification, Bacteria isolation & purification, Periodontitis microbiology
- Abstract
The purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (RPP). Subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. The examination of the subgingival microflora indicated that certain species, including Porphyromonas gingivalis, Bacteroides forsythus, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Campylobacter species were found to be predominant in severe periodontal lesions. B. forsythus, P. gingivalis, Prevotella intermedia, F. nucleatum, Capnocytophaga ochracea, were predominant in medium lesions while Streptococcus species and Actinomyces species, C. ochracea, Haemophilus segnis and Veillonella parvula, were found in higher levels in minimal periodontal lesions.
- Published
- 1995
- Full Text
- View/download PDF
30. Microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.
- Author
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Kamma JJ, Nakou M, and Manti FA
- Subjects
- Adult, Aggregatibacter actinomycetemcomitans isolation & purification, Aggressive Periodontitis microbiology, Aggressive Periodontitis pathology, Analysis of Variance, Bacteroides isolation & purification, Campylobacter isolation & purification, Capnocytophaga isolation & purification, Colony Count, Microbial, Dental Plaque microbiology, Female, Fusobacterium nucleatum isolation & purification, Gingival Hemorrhage, Humans, Likelihood Functions, Linear Models, Male, Peptostreptococcus isolation & purification, Periodontal Index, Periodontal Pocket microbiology, Periodontal Pocket pathology, Porphyromonas gingivalis isolation & purification, Prevotella intermedia isolation & purification, Statistics, Nonparametric, Streptococcus isolation & purification, Streptococcus sanguis isolation & purification, Suppuration, Periodontitis microbiology, Periodontitis pathology
- Abstract
The microbial population in 73 rapidly progressive periodontitis (RPP) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. Porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by Bacteroides forsythus (23.6%), and Prevotella intermedia (15.7%). The prevalence of P. gingivalis was 91.7%, Fusobacterium nucleatum, 90.4%; Streptococcus intermedius, 87.7%; and B. forsythus, 53.4%. Significant differences between bleeding index 0, 1, and 2 (P < 0.05) in frequency of detection were found for P. intermedia, Campylobacter concisus, Selenomonas sputigena, and Peptostreptococcus micros at bleeding sites and for Streptococcus sanguis, Actinobacillus actinomycetemcomitans, and B. forsythus (P < 0.001) at non-bleeding sites. Between suppurating and non-suppurating sites, significant differences (P < 0.05) in frequency of isolation were found for P. intermedia, Capnocytophaga ochracea, and A. actinomycetemcomitans at suppurating sites and for F. nucleatum at non-suppurating sites.
- Published
- 1994
- Full Text
- View/download PDF
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