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2. In-air microfluidics enables rapid fabrication of emulsions, suspensions, and 3D modular (bio)materials

Author

Visser, Claas Willem, Kamperman, Tom, Karbaat, Lisanne P., Lohse, Detlef, and Karperien, Marcel

Subjects
Physics - Fluid Dynamics, Physics - Applied Physics
Abstract

Microfluidic chips provide unparalleled control over droplets and jets, which have advanced all natural sciences. However, microfluidic applications could be vastly expanded by increasing the per-channel throughput and directly exploiting the output of chips for rapid additive manufacturing. We unlock these features with in-air microfluidics, a new chip-free platform to manipulate microscale liquid streams in the air. By controlling the composition and in-air impact of liquid microjets by surface tension-driven encapsulation, we fabricate monodisperse emulsions, particles, and fibers with diameters of 20 to 300um at rates that are 10 to 100 times higher than chip-based droplet microfluidics. Furthermore, in-air microfluidics uniquely enables module-based production of three-dimensional (3D) multiscale (bio)materials in one step because droplets are partially solidified in-flight and can immediately be printed onto a substrate. In-air microfluidics is cytocompatible, as demonstrated by additive manufacturing of 3D modular constructs with tailored micro-environments for multiple cell types. Its in-line control, high throughput and resolution, and cytocompatibility make in-air microfluidics a versatile platform technology for science, industry, and health care.

Published
2020
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9. Tunable and Compartmentalized Multimaterial Bioprinting for Complex Living Tissue Constructs

Author

Hassan, Shabir, primary, Gomez-Reyes, Eduardo, additional, Enciso-Martinez, Eduardo, additional, Shi, Kun, additional, Campos, Jorge Gonzalez, additional, Soria, Oscar Yael Perez, additional, Luna-Cerón, Eder, additional, Lee, Myung Chul, additional, Garcia-Reyes, Isaac, additional, Steakelum, Joshua, additional, Jeelani, Haziq, additional, García-Rivera, Luis Enrique, additional, Cho, Minsung, additional, Cortes, Stephanie Sanchez, additional, Kamperman, Tom, additional, Wang, Haihang, additional, Leijten, Jeroen, additional, Fiondella, Lance, additional, and Shin, Su Ryon, additional

Published
2022
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10. Embedded 3D Printing in Self‐Healing Annealable Composites for Precise Patterning of Functionally Mature Human Neural Constructs (Adv. Sci. 25/2022)

Author

Kajtez, Janko, primary, Wesseler, Milan Finn, additional, Birtele, Marcella, additional, Khorasgani, Farinaz Riyahi, additional, Rylander Ottosson, Daniella, additional, Heiskanen, Arto, additional, Kamperman, Tom, additional, Leijten, Jeroen, additional, Martínez‐Serrano, Alberto, additional, Larsen, Niels B., additional, Angelini, Thomas E., additional, Parmar, Malin, additional, Lind, Johan U., additional, and Emnéus, Jenny, additional

Published
2022
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13. Tethering Cells via Enzymatic Oxidative Crosslinking Enables Mechanotransduction in Non‐Cell‐Adhesive Materials (Adv. Mater. 42/2021)

Author

Kamperman, Tom, primary, Henke, Sieger, additional, Crispim, João F., additional, Willemen, Niels G. A., additional, Dijkstra, Pieter J., additional, Lee, Wooje, additional, Offerhaus, Herman L., additional, Neubauer, Martin, additional, Smink, Alexandra M., additional, Vos, Paul, additional, Haan, Bart J., additional, Karperien, Marcel, additional, Shin, Su Ryon, additional, and Leijten, Jeroen, additional

Published
2021
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14. Embedded 3D printing in self-healing annealable composites for functional patterning of human neural constructs

Author

Kajtez, Janko, Wessler, Milan Finn, Birtele, Marcella, Khorasgani, Farinaz Riyahi, Rylander Ottosson, Daniella, Heiskanen, Arto, Kamperman, Tom, Leijten, Jeroen, Martínez-Serrano, Alberto, Larsen, Niels B., Angelini, Thomas E., Parmar, Malin, Lind, Johan U., Emnéus, Jenny, TechMed Centre, and Developmental BioEngineering

Abstract

Human in vitro models of neural tissue with controllable cellular composition, tunable microenvironment, and defined spatial patterning are needed to facilitate studies of brain development and disease. Towards this end, bioprinting has emerged as a promising strategy. However, precise and programmable printing of extremely soft and compliant materials that are permissive for stem cell differentiation and functional neuronal growth has been a major challenge. Therefore, solutions for engineering structurally and functionally defined human neural constructs remain scarce. Here, we present a modular platform for bioengineering of neuronal networks via direct embedded 3D printing of human stem cells inside Self-Healing Annealable Particle-Extracellular matrix (SHAPE) composites. The approach combines rheological benefits of granular soft microgel supports with the versatile biomimicry of bulk hydrogels to simultaneously enable precise freeform patterning of stem cells, and consequent generation and long-term maintenance of subtype-specific neurons within engineered networks that extend into the bulk of the annealed support. The developed approach further allows multi-ink deposition, live spatial and temporal monitoring of oxygen levels, as well as creation of vascular channels. Due to its modularity, SHAPE biomanufacturing toolbox not only offers a solution for functional modeling of mechanically sensitive neural constructs, but also has potential to be applied to a wide range of biomaterials with different crosslinking mechanisms to model tissues and diseases where recapitulation of complex architectural features and topological cues is essential.

Published
2021

15. Embedded 3D printing in self-healing annealable composites for precise patterning of functionally mature human neural constructs

Author

Kajtez, Janko, primary, Wesseler, Milan Finn, additional, Birtele, Marcella, additional, Khorasgani, Farinaz Riyahi, additional, Ottosson, Daniella Rylander, additional, Heiskanen, Arto, additional, Kamperman, Tom, additional, Leijten, Jeroen, additional, Martínez-Serrano, Alberto, additional, Larsen, Niels B., additional, Angelini, Thomas E., additional, Parmar, Malin, additional, Lind, Johan U., additional, and Emnéus, Jenny, additional

Published
2021
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16. hiPSC-derived 3D Bioprinted Skeletal Muscle Tissue Implants Regenerate Skeletal Muscle Following Volumetric Muscle Loss

Author

Jodat, Yasamin A., Zhang, Ting, Tanoury, Ziad Al, Kamperman, Tom, Shi, Kun, Huang, Yike, Panayi, Adriana, Endo, Yori, Wang, Xichi, Quint, Jacob, Arnaout, Adnan, Kiaee, Kiavash, Hassan, Shabir, Lee, Junmin, Martinez, Angel Flores Huidobro, Ochoa, Sofia Lara, Lee, KangJu, Calabrese, Michelle, Carlucci, Alessandro, Tamayol, Ali, Sinha, Indranil, Pourquié, Olivier, Ryon Shin, Su, Developmental BioEngineering, and TechMed Centre

Abstract

Engineering of biomimetic tissue implants provides an opportunity for repairing volumetric muscle loss (VML), beyond a tissue’s innate repair capacity. Here, we present thick, suturable, and pre-vascularized 3D muscle implants containing human induced pluripotent stem cell-derived myogenic precursor cells (hiPSC-MPCs), which can differentiate into skeletal muscle cells while maintaining a self-renewing pool. The formation of contractile myotubes and millimeter-long fibers from hiPSC-MPCs is achieved in chemically, mechanically, and structurally tailored extracellular matrix-based hydrogels, which can serve as scaffolds to ultimately organize the linear fusion of myoblasts. Embedded multi-material bioprinting is used to deposit complex patterns of perfusable vasculatures and aligned hiPSC-MPC channels within an endomysium-like supporting gel to recapitulate muscle architectural integrity in a facile yet highly rapid manner. Moreover, we demonstrate successful graft-host integration and de novo muscle formation upon in vivo implantation of pre-vascularized constructs within a VML model. This work pioneers the engineering of large pre-vascularized hiPSC-derived muscle tissues toward next generation VML regenerative therapies.

Published
2021

17. hiPSC-derived 3D Bioprinted Skeletal Muscle Tissue Implants Regenerate Skeletal Muscle Following Volumetric Muscle Loss

Author

Jodat, Yasamin A., primary, Zhang, Ting, additional, Tanoury, Ziad Al, additional, Kamperman, Tom, additional, Shi, Kun, additional, Huang, Yike, additional, Panayi, Adriana, additional, Endo, Yori, additional, Wang, Xichi, additional, Quint, Jacob, additional, Arnaout, Adnan, additional, Kiaee, Kiavash, additional, Hassan, Shabir, additional, Lee, Junmin, additional, Martinez, Angel Flores Huidobro, additional, Ochoa, Sofia Lara, additional, Lee, KangJu, additional, Calabrese, Michelle, additional, Carlucci, Alessandro, additional, Tamayol, Ali, additional, Sinha, Indranil, additional, Pourquié, Olivier, additional, and Shin, Su Ryon, additional

Published
2021
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20. Engineering 3D parallelized microfluidic droplet generators with equal flow profiles by computational fluid dynamics and stereolithographic printing

Author

Kamperman, Tom, primary, Teixeira, Liliana Moreira, additional, Salehi, Seyedeh Sarah, additional, Kerckhofs, Greet, additional, Guyot, Yann, additional, Geven, Mike, additional, Geris, Liesbet, additional, Grijpma, Dirk, additional, Blanquer, Sebastien, additional, and Leijten, Jeroen, additional

Published
2020
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21. 3D Printed Tissues: 3D Printed Cartilage‐Like Tissue Constructs with Spatially Controlled Mechanical Properties (Adv. Funct. Mater. 51/2019)

Author

Melo, Bruna A. G., primary, Jodat, Yasamin A., additional, Mehrotra, Shreya, additional, Calabrese, Michelle A., additional, Kamperman, Tom, additional, Mandal, Biman B., additional, Santana, Maria H. A., additional, Alsberg, Eben, additional, Leijten, Jeroen, additional, and Shin, Su Ryon, additional

Published
2019
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29. Microfluidics for Complex Tissue Engineering

Author

Kamperman, Tom, Henke, S.J., Karperien, Hermanus Bernardus Johannes, Developmental BioEngineering, and Faculty of Science and Technology

Subjects
METIS-321615, IR-103933
Published
2016

36. Scalable fabrication, compartmentalization and applications of living microtissues

Author

Schot, Maik, Araújo-Gomes, Nuno, van Loo, Bas, Kamperman, Tom, and Leijten, Jeroen

Abstract

Living microtissues are used in a multitude of applications as they more closely resemble native tissue physiology, as compared to 2D cultures. Microtissues are typically composed of a combination of cells and materials in varying combinations, which are dictated by the applications’ design requirements. Their applications range wide, from fundamental biological research such as differentiation studies to industrial applications such as cruelty-free meat production. However, their translation to industrial and clinical settings has been hindered due to the lack of scalability of microtissue production techniques. Continuous microfluidic processes provide an opportunity to overcome this limitation as they offer higher throughput production rates as compared to traditional batch techniques, while maintaining reproducible control over microtissue composition and size. In this review, we provide a comprehensive overview of the current approaches to engineer microtissues with a focus on the advantages of, and need for, the use of continuous processes to produce microtissues in large quantities. Finally, an outlook is provided that outlines the required developments to enable large-scale microtissue fabrication using continuous processes.

Published
2022
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37. 3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties.

Author

de Melo BAG, Jodat YA, Mehrotra S, Calabrese MA, Kamperman T, Mandal BB, Santana MHA, Alsberg E, Leijten J, and Shin SR

Abstract

Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is bioprinted using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a hard biomaterial (MPa order compressive modulus) composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel is developed as an extracellular matrix (ECM) with self-healing properties, but low diffusive capacity. Within this bath supplemented with thrombin, fibrinogen containing human mesenchymal stem cell (hMSC) spheroids is bioprinted forming fibrin, as the soft biomaterial (kPa order compressive modulus) to simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids improve viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to print locally soft and cell stimulating microenvironments inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage., Competing Interests: Conflict of Interest The authors declare no conflict of interest.

Published
2019
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