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18 results on '"Kanazir, Dusan T."'

3. Effects of age and dexamethasone treatment on glucocorticoid response element and activating protein-1 binding activity in rat brain

Author

Terzic, Natasa, Vujcic, Miroslava, Ristic-Fira, Aleksandra, Krstic-Demonacos, Marija, Milanovic, Desanka, Kanazir, Dusan T., and Ruzdijic, Sabera

Subjects
Gene expression -- Research, Dexamethasone, Corticosteroids, Health, Seniors
Abstract

The effect of dexamethasone (DEX) on glucocorticoid receptor (GR)-mediated gene expression was examined in the brain of young and aged rats. Electrophoretic mobility shift assays showed that DEX treatment led to an increase of glucocorticoid response element (GRE) binding activity in aged rats, whereas in young animals GRE binding activity was decreased. Western blot analysis and reverse transcriptase polymerase chain reaction confirmed that, in aged animals, the GR mRNA and the GR protein levels were increased on DEX treatment. The binding activity of GRE activating protein-1 (AP-l) site and cross-competition analysis demonstrated specific pattern of expression during the ageing and DEX treatment, suggesting that GR modulates the activity of transcription factors AP-1 (Fos/Jun proteins) through protein--protein interaction. On the basis of these results, it can be concluded that the composition of transcriptional complexes that bind to GRE and AP-1 regulatory elements changes upon DEX treatment in an age-specific manner.

Published
2003

11. Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae

Author

Popović, Nataša M., Ruždijić, Sabera, Kanazir, Dusan T., Nićiforović, Ana, Adžić, Miroslav, Paraskevopoulou, Elissavet, Pantelidou, Constantia, Radoičić, Marija B., Demonacos, Constantinos, Krstić-Demonacos, Marija, Popović, Nataša M., Ruždijić, Sabera, Kanazir, Dusan T., Nićiforović, Ana, Adžić, Miroslav, Paraskevopoulou, Elissavet, Pantelidou, Constantia, Radoičić, Marija B., Demonacos, Constantinos, and Krstić-Demonacos, Marija

Abstract

The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.

Published
2010

12. Inheritable Differences in the Electrophoretic Pattern of Hemoglobin Revealed in a Local Random-Bred Colony of Albino Rats

Author

MARTINOVIC, JOVO V., MARINKOVIC, DOBRIVOJE V., KANAZIR, DUSAN T., and MARTINOVITCH, PETER N.

Abstract

In a local colony of random-bred Albino rats, three different patterns of hemoglobin, arbitrarily denoted as patterns I, II and III, were detected by means of starch-gel electrophoresis in a discontinuous buffer system. Mating experiments showed that rats bearing pattern I and pattern III hemoglobin bred true. Crosses of pattern I with pattern III animals yielded only pattern II animals, and when the latter were mated inter se, the resultant F2 generation showed approximately a ratio of 1:2:1 for patterns I, II and III, respectively. When F1 animals from pattern I with pattern III crosses were mated back to animals of either parental type, the resultant ratio was found to be one pattern II: one pattern I or pattern III.

Published
1970
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18. Role of antioxidant enzymes in radiosensitivity of human blood cells

Author

Joksic, Gordana, Kanazir, Dusan T., Pejic, Snezana, Pajovic, Snezana B., and Kasapovic, Jelena

Subjects
RADIATION exposure
Abstract

The marked variability in radiation response among individuals of the same age group prompted us to investigate the role of antioxidativeenzyme activity. Micronuclei (MN) and enzyme assays were performed on blood samples of healthy male volunteers. The procedure consisted of micronucleus analysis and measurement of the superoxide dismutase (SOD) activity in harvested blood samples irradiated in vitro with 2 Gy Gamma-rays and in unirradiated control samples for each individual.We found that the yield of radiation-induced micronuclei was in the range of 112 to 378 micronuclei per 1000 binucleated cells. The activity of cytosol superoxide dismutase (CuZnSOD) was reduced, whereas the activity of manganese superoxide dismutase (MnSOD) was markedly elevated in the blood samples harvested in lymphocyte cultures after irradiation. The analysis of our results showed that MnSOD plays the most important role in radiation-induced cellular damage. The results ofthis investigation showed that measurement of micronuclei and the activities of SOD in harvested human blood cells can serve as a rapid predictive assay of radiosensitivity in a clinical setting. [ABSTRACT FROM AUTHOR]

Published
2000

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