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Optical coherence tomography (OCT) enables three-dimensional imaging with resolution on the micrometer scale. The technique relies on the time-of-flight gated detection of light scattered from a sample and has received enormous interest in applications as versatile as non-destructive testing, metrology and non-invasive medical diagnostics. However, in strongly scattering media such as biological tissue, the penetration depth and imaging resolution are limited. Combining OCT imaging with wavefront shaping approaches significantly leverages the capabilities of the technique by controlling the scattered light field through manipulation of the field incident on the sample. This article reviews the main concepts developed so far in the field and discusses the latest results achieved with a focus on signal enhancement and imaging.

Recently the acquisition of the time-resolved reflection matrix was demonstrated based on spectral domain optical coherence tomography. In principle, the matrix describes the linear dependence of the OCT signal received from different depths on the field which is incident to the scattering sample. Knowledge of the matrix, hence, enables beam shaping to selectively enhance the received signal, for example to increase the penetration depth when imaging turbid media. We investigate the impact of image artefacts on the approach. Phase conjugation is shown to enhance the OCT signal, but not autocorrelation and mirror artefacts. Imaging applications are demonstrated indicating the potential for future in-vivo studies on biotissues.

Spectral domain optical coherence tomography (SD-OCT) is a highly versatile method which allows for three dimensional optical imaging in scattering media. A number of recent publications demonstrated the technique to benefit from structured illumination and beam shaping approaches, e.g. to enhance the signal-to-noise ratio or the penetration depth with samples such as biological tissue. We present a compact and easy to implement design for independent wavefront manipulation and beam shaping at the reference and sample arm of the interferometric OCT device. The design requires a single spatial light modulator and can be integrated to existing free space SD-OCT systems by modifying the source arm only. We provide analytical and numerical discussion of the presented design as well as experimental data confirming the theoretical analysis. The system is highly versatile and lends itself for applications where independent phase or wavefront control is required. We demonstrate the system to be used for wavefront sensorless adaptive optics as well as for iterative optical wavefront shaping for OCT signal enhancement in strongly scattering media.

In this article, we present a simple and intuitive approach to create a handheld optoacoustic setup for near field measurements. A single piezoelectric transducer glued in between two sheets of polymethyl methacrylate (PMMA) facilitates nearfield depth profiling of layered media. The detector electrodes are made of indium tin oxide (ITO) which is both electrically conducting as well as optically transparent, enabling an on-axis illumination through the detector. By mapping the active detector area, we show that it matches the design form precisely. We also present a straightforward approach to determine the instrument response function, which allows to obtain the original pressure profile arriving at the detector. To demonstrate the validity of this approach, the measurement on a simple test sample is deconvolved with the instrument response function and compared to simulation results. Except for the sputter instrumentation, all required materials and instruments as well as the tools needed to create such a setup are available to standard scientific laboratories.

We propose a compact setup for wavefront manipulation in spectral domain optical coherence tomography (OCT). The system can easily be implemented into existing free-space OCT setups through modification of the source path only. We demonstrate complex-valued OCT signal acquisition based on phase shifting combined with iterative optical wavefront shaping, which locally enhances the OCT signal acquired from within a scattering sample. The system lends itself to future imaging studies in strongly scattering media such as biological tissue.