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In remote sensing, the conversion of at-sensor radiance to surface reflectance for each pixel in a scene is an essential component of many analysis tasks. The empirical line method (ELM) is the most used technique among remote sensing practitioners due to its reliability and production of accurate reflectance measurements. However, the at-altitude radiance ratio (AARR), a more recently proposed methodology, is attractive as it allows reflectance conversion to be carried out in real time throughout data collection, does not require calibrated samples of pre-measured reflectance to be placed in scene, and can account for changes in illumination conditions. The benefits of AARR can substantially reduce the level of effort required for collection setup and subsequent data analysis, and provide a means for large-scale automation of remote sensing data collection, even in atypical flight conditions. In this study, an onboard, downwelling irradiance spectrometer integrated onto a small unmanned aircraft system (sUAS) is utilized to characterize the performance of AARR-generated reflectance from hyperspectral radiance data under a variety of challenging illumination conditions. The observed error introduced by AARR is often on par with ELM and acceptable depending on the application requirements and natural variation in the reflectance of the targets of interest. Additionally, a number of radiometric and atmospheric corrections are proposed that could increase the accuracy of the method in future trials, warranting further research.

We demonstrate optical manipulation of structures and defects in liquid crystals (LCs). The effective refractive index depends on the LC molecular orientations and the laser beam's polarization. We use the orientation-mediated refractive index contrast for the laser trapping in LCs with a homogeneous composition, but with spatially-varying patterns of molecular orientations. Tightly-focused polarized beams allow for optical trapping of disclinations and their clusters, dislocations and oily streaks, cholesteric fingers and focal conic domains, etc. We calculate the optical gradient forces for typical structures and explain the trapping properties at low laser powers. We also show that when a high-power beam causes local molecular realignment, the laser trapping properties change for two reasons: (1) the refractive index pattern and optical gradient forces are modified; (2) additional elastic structural forces arise to minimize the elastic free energy.

A multiple-trap single-beam scanning laser tweezer system was developed and characterized. Different stationary and mobile multiple-trap modes were generated for polystyrene beads in a water environment. Trapping efficiency and stability were investigated for several dynamic parameters such as transition time between the sites, waiting time on a single site, number of trapping sites, and IR laser power. Optimal parameters for efficient generation of complex arrays and matrices were determined. We demonstrate an example of a single laser beam multiple-trap application by measuring the trap's stiffness in water for our laser tweezer setup.

A novel technique for the calibration of laser trapping systems that utilizes two-photon-excited fluorescence of commercial dye-stained microspheres has been demonstrated. The trapping forces as well as the trapping efficiency have been measured for various liquid environments and trapping depths. The trapping efficiency in water was found to decrease with an increase of trapping depths because of the enlargement of the trapping beam waist caused by aberrations of the optical system.