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1. Using Polarized Spectroscopy to Investigate Order in Thin-Films of Ionic Self-Assembled Materials Based on Azo-Dyes

Author

Miguel R. Carro-Temboury Martin Kühnel, Mariam Ahmad, Frederik Andersen, Ári Brend Bech, H. Krestian L. Bendixen, Patrick R. Nawrocki, Anders J. Bloch, Ilkay Bora, Tahreem A. Bukhari, Nicolai V. Bærentsen, Jens Carstensen, Smeeah Chima, Helene Colberg, Rasmus T. Dahm, Joshua A. Daniels, Nermin Dinckan, Mohamed El Idrissi, Ricci Erlandsen, Marc Førster, Yasmin Ghauri, Mikkel Gold, Andreas Hansen, Kenn Hansen, Mathias Helmsøe-Zinck, Mathias Henriksen, Sophus V. Hoffmann, Louise O. H. Hyllested, Casper Jensen, Amalie S. Kallenbach, Kirandip Kaur, Suheb R. Khan, Emil T. S. Kjær, Bjørn Kristiansen, Sylvester Langvad, Philip M. Lund, Chastine F. Munk, Theis Møller, Ola M. Z. Nehme, Mathilde Rove Nejrup, Louise Nexø, Simon Skødt Holm Nielsen, Nicolai Niemeier, Lasse V. Nikolajsen, Peter C. T. Nøhr, Dominik B. Orlowski, Marc Overgaard, Jacob Skaarup Ovesen, Lucas Paustian, Adam S. Pedersen, Mathias K. Petersen, Camilla M. Poulsen, Louis Praeger-Jahnsen, L. Sonia Qureshi, Nicolai Ree, Louise S. Schiermacher, Martin B. Simris, Gorm Smith, Heidi N. Smith, Alexander K. Sonne, Marko R. Zenulovic, Alma Winther Sørensen, Karina Sørensen, Emil Vogt, Andreas Væring, Jonas Westermann, Sevin B. Özcan, and Thomas Just Sørensen

Subjects
ionic self-assembly, thin films, molecular orientation, non-covalent forces, soft materials, Chemistry, QD1-999
Abstract

Three series of ionic self-assembled materials based on anionic azo-dyes and cationic benzalkonium surfactants were synthesized and thin films were prepared by spin-casting. These thin films appear isotropic when investigated with polarized optical microscopy, although they are highly anisotropic. Here, three series of homologous materials were studied to rationalize this observation. Investigating thin films of ordered molecular materials relies to a large extent on advanced experimental methods and large research infrastructure. A statement that in particular is true for thin films with nanoscopic order, where X-ray reflectometry, X-ray and neutron scattering, electron microscopy and atom force microscopy (AFM) has to be used to elucidate film morphology and the underlying molecular structure. Here, the thin films were investigated using AFM, optical microscopy and polarized absorption spectroscopy. It was shown that by using numerical method for treating the polarized absorption spectroscopy data, the molecular structure can be elucidated. Further, it was shown that polarized optical spectroscopy is a general tool that allows determination of the molecular order in thin films. Finally, it was found that full control of thermal history and rigorous control of the ionic self-assembly conditions are required to reproducibly make these materials of high nanoscopic order. Similarly, the conditions for spin-casting are shown to be determining for the overall thin film morphology, while molecular order is maintained.

Published
2018
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2. Abstracts of presentations on plant protection issues at the fifth international Mango Symposium Abstracts of presentations on plant protection issues at the Xth international congress of Virology

Author

J. E. Peña, M. Wysoki, Gajendra Singh, Nancy Boscán de M., Freddy J. Godoy, A. Obligado, C. J. Rossetto, I. J. A. Ribeiro, P. B. Gallo, N. B. Soares, J. C. Sabino, A. L. M. Martins, N. Bortoletto, R. C. Ploetz, D. Benscher, Aimé Vázquez, A. Colls, Julianne Nagel, B. Schaffer, Y. Pinkas, M. Maymon, S. Freeman, Mikhail Bostros Bastawros, M. J. Gosbee, G. I. Johnson, D. C. Joyce, J. A. G. Irwin, W. C. Saaiman, D. Prusky, E. Falik, I. Kobiler, Y. Fuchs, G. Zauberman, E. Pesis, M. Ackerman, I. Roth, A. Weksler, O. Yekutiely, A. Waisblum, A. Keinan, G. Ofek, R. Reved, R. Barak, P. Bel, L. Artes, N. Visarathanonth, Z. Xu, L. Ponce de León, C. Muñoz, L. Pérez, F. Diaz de León, C. Kerbel, S. Esparza, E. Bósquez, M. Trinidad, L. M. Coates, A. W. Cooke, J. R. Dean, Ana Lucia Duarte, Paulo Alberto Otto, Aldo Malavasi, M. C. C. Lizado, M. L. Bautista, L. A. Artes, N. S. Bacalangco, U. Farungsang, N. Farungsang, D. P. Waskar, S. D. Masalkar, R. S. Gaikwad, S. V. Damame, Ian S. E. Bally, Tim J. O’Hare, Rowland J. Holmes, J. G. Atabekov, Claude M. Fauquet, O. Tomori, D. L. Nuss, P. Ahlquist, J. Díez, M. Ishikawa, M. Janda, B. D. Price, M. Restrepo-Hartwig, J. F. Bol, C. M. A. van Rossum, M. L. Garcia, E. A. G. van der Vossen, Chantal B. E. M. Reusken, T. R. Canto, A. Gal-On, P. Palukaitis, M. J. Roossinck, S. Flasinski, Maria A. Restrepo-Hartwig, Paul Ahlquist, Ekaterina Smirnyagina, Na-Sheng Lin, Peter D. Nagy, Marek Figlerowicz, Jozef J. Bujarski, D. F. Proll, K. J. Guyatt, A. D. Davidson, Kook-Hyung Kim, Eric Miller, Cynthia Hemenway, Z. Havelda, T. Dalmay, J. Burgyán, C. M. Kearney, M. Thomson, K. E. Roland, W. O. Dawson, Y. Bao, S. A. Carter, R. S. Nelson, P. M. Derrick, Xin Shun Ding, J. K. Eskarous, S. Sarkar, M. El-Shamy, J. Chen, N. Sako, W. Yuichiro, K. Ohshima, Y. Okada, Brice Felden, Yuri G. Kuznetsov, Alexander J. Malkin, Aaron Greenwood, Alexander McPherson, K. I. Ivanov, Y. L. Dorokhov, C. H. Kim, Katalin Sálanki, Isabelle Carrére, Mireille Jacquemond, Mark Tepfer, Ervin Balazs, A. I. Sanz, M. T. Serra, I. García-Luque, F. Revers, T. Candresse, O. LeGall, S. Souche, H. Lot, J. Dunez, E. Cecchini, J. Milner, N. Al-Kaff, S. Covey, Z. Gong, C. Geri, S. N. Covey, K. R. Richert-Pöggeler, R. J. Shepherd, R. Casper, Eti Meiri, B. Raccah, A. Gera, S. Singer, E. K. Allam, Soheir I. El Afifi, M. A. Abo El Nasr, M. H. Abd El Ghaffar, I. Elisabeth Johansen, K. E. Keller, R. O. Hampton, Karina SÕrensen, S. S. Bishnoi, Narayan Rishi, M. Y. D. Gumedzoe, K. Atissime, S. Yedibahoma, Joan Wellink, Jan Verver, Peter Bertens, Jan van Lent, Rob W. Goldbach, Ab van Kammen, Annemarie Lekkerkerker, K. M. Taylor, V. E. Spall, G. P. Lomonossoff, S. Yu. Morozov, A. G. Solovyev, D. A. Zelenina, E. I. Savenkov, V. Z. Grdzelishvili, S. Y. Morozov, K. A. J. Jansen, C. J. A. M. Wolfs, H. Lohuis, B. J. M. Verduin, V. A. Stein-Margolina, Y. H. Hsu, B. Y. Chang, N. S. Lin, Marcel Pilartz, Holger Jeske, Jeanmarie Verchot, David C. Baulcombe, David J. English, E. Müller, D. C. Baulcombe, Isabelle Malcuit, Tony Kavanagh, J. P. T. Valkonen, Ü. Puurand, A. Merits, F. Rabinstein, O. Sorri, M. Saarma, Y. C. Liao, C. Vaquero-Martin, M. Monecke, W. Rohde, D. Prüfer, R. Fischer, Y. Antignus, O. Lachman, M. Pearlsman, S. Cohen, W. P. Qiu, J. W. Moyer, A. Feldhoff, M. Kikkert, R. Kormelink, G. Krczal, D. Peters, György Szittya, József Burgyán, K. Wvpijewski, E. Paduch-Cichal, A. Rezler, S. Skrzeczkowska, J. Augustyniak, L. Nemchinov, E. Maiss, A. Hadidi, Anita Wittner, László Palkovics, Ervin Balázs, A. Crescenzi, P. Piazzolla, A. Kheyr-Pour, G. A. Dafalla, H. Lecoq, B. Gronenborn, U. Bauer, I. Laux, M. R. Hajimorad, X. S. Ding, Stanislaw Flasinski, Pour G. Cassidy, B. Dugdale, P. R. Beetham, R. M. Harding, J. L. Dale, G. Qiu, J. G. Shaw, A. Molnár, P. Más, J. M. Balsalobre, M. A. Sánchez-Pina, V. Pallás, J. Rahontei, L. López, J. J. Lázara, M. Barón, R. A. Owens, G. Steger, Y. Hu, A. Fels, R. W. Hammond, D. Riesner, A. R. W. Schröder, A. Góra, J. Pawlowicz, A. Kierzek, W. Zagorski, T. Baumstark, W. Schiebel, R. Schiebel, A. Axmann, B. Haas, H. L. Sänger, Yang Xicai, Yie Yin, Zhu Feng, Liu Yule, Kang Liangyi, Tien Po, H. Poliyka, U. Staub, M. Wagner, H. J. Gross, Teruo Sano, Akiro Ishiguro, J. Fayos, R. Garro, J. M. Bellés, V. Conejero, R. G. Bonfiglioli, D. R. Webb, R. H. Symons, K. A. El-Dougdoug, A. A. Abo-Zeid, S. Ambrós, C. Hernandez, J. C. C. Desvignes, R. Flores, M. d’Aquilio, V. Lisa, G. Boccardo, A. Vera, J. A. Daròs, J. Henkel, R. Spieker, C. Higgins, R. Turley, D. Chamberlain, M. Bateson, J. Dale, L. d’Aquino, A. Ragozzino, J. Henderson, M. F. Bateson, W. Chaleeprom, A. J. Gibbs, K. Graichen, F. Rabenstein, E. Schliephake, H. G. Smith, M. Stevens, E. Sadowy, D. Hulanicka, B. Wegener, M. T. Martin, T. Wetzel, G. Cook, G. G. F. Kasdorf, G. Pietersen, Kathryn S. Braithwaite, Cherie F. Gambley, Grant R. Smith, Arnis Druka, Lucille Villegas, Ganesh Dahal, Roger Hull, N. A. Senchugova, C. Büchen-Osmond, M. J. Dallwitz, L. D. Blaine, P. S. Naik, A. B. Sonone, A. S. Kolaskar, J. Y. Sgro, A. C. Palmenberg, Denis Leclerc, Thomas Hohn, E. Moriones, A. Batlle, M. Luis, J. Alvarez, J. J. Bernal, J. L. Alonso, J. Spak, D. Kubelkova, T. T. Kuo, K. K. Gachechiladze, R. S. Adamia, N. S. Balardshishvili, T. G. Chanishvili, D. H. Krüger, Tibor Nagy, Péter Élö, Péter Papp, László Orosz, N. Licis, V. Berzins, Carlos A. Sariol-Carbelo, C. M. RodrCarlos, D. Janzen, Colin W. Ward, S. W. Scott, P. J. Shiel, P. H. Berger, M. E. Aleman, R. N. Beachy, C. M. Fauquet, S. N. Salm, E. P. Rybicki, M. E. C. Rey, R. W. Briddon, G. Harper, A. Druka, S. Phillips, A. A. Brunt, R. Hull, Jo Hay, Indranil Dasgupta, Fan Zaifeng, Brian M. Meehan, Daniel Todd, Hans-Jörk Bunk, F. Grieco, G. P. Martelli, P. Saldarelli, A. Minafra, A. Morag, M. Mumcuoglu, T. Baybikov, M. Schlesinger, Z. Zakay-Rones, B. Shohat, M. Shohat, M. Miller, M. Shaklay, Z. Kalvatchev, R. Walder, D. Garzaro, M. Barrios, Ali Karagöz, Avni Kuru, M. R. Karim, A. J. Johnson, S. Takida, M. C. Thompson, H. M. K. Omer, O. L. M. Omer, L. Biyiti, R. H. Amvam, G. Lamaty, P. Bouchet, J. Xu, K. L. Hefferon, M. G. Abou Haidar, and A. X. X. Meng

Subjects
0106 biological sciences, Zucchini yellow mosaic virus, Barley stripe mosaic virus, biology, Ecology (disciplines), Plant Science, Coat protein, biology.organism_classification, 01 natural sciences, Cucumber mosaic virus, 010602 entomology, Insect Science, International congress, Botany, 010606 plant biology & botany
Published
1997
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3. Screening of congenital heart disease patients using multiplex ligation-dependent probe amplification: early diagnosis of syndromic patients

Author

Jan P. Schouten, Eva Fernlund, Maria Soller, Jesper Steensberg, Mohaddeseh Behjati, Niels Tommerup, Paal Skytt Andersen, Karina SÕrensen, Lars Allan Larsen, Milad El-Segaier, Maria Kirchoff, Patrice Bouvagnet, Nancy Nehme, Vibeke E. Hjortdal, Thomas Werge, and Ab Errami

Subjects
Oncology, Heart Defects, Congenital, Male, congenital, hereditary, and neonatal diseases and abnormalities, medicine.medical_specialty, endocrine system diseases, Heart disease, Adolescent, DNA Copy Number Variations, Heart Diseases, Gene Dosage, Bioinformatics, Internal medicine, mental disorders, Genetics, medicine, Humans, Multiplex, Multiplex ligation-dependent probe amplification, Copy-number variation, Stage (cooking), Child, Genetics (clinical), Aged, Chromosome Aberrations, business.industry, Infant, Newborn, Infant, medicine.disease, Child, Preschool, Medical genetics, Female, business, Multiplex Polymerase Chain Reaction, Nucleic Acid Amplification Techniques, Pediatric cardiology
Abstract

Recurrent copy number variants (CNVs) are found in a significant proportion of patients with congenital heart disease (CHD) and some of these CNVs are associated with other developmental defects. In some syndromic patients, CHD may be the first presenting symptom, thus screening of patients with CHD for CNVs in specific genomic regions may lead to early diagnosis and awareness of extracardiac symptoms. We designed a multiplex ligation-dependent probe amplification (MLPA) assay specifically for screening of CHD patients. The MLPA assay allows for simultaneous analysis of CNVs in 25 genomic regions previously associated with CHD. We screened blood samples from 402 CHD patients and identified 14 rare CNVs in 13 (3.2%) patients. Five CNVs were de novo and six where inherited from a healthy parent. The MLPA screen led to early syndrome diagnosis in two of these patients. We conclude that the MLPA assay detects clinically relevant CNVs and suggest that it could be used within pediatric cardiology as a first tier screen to detect clinically relevant CNVs and identify syndromic patients at an early stage. (C) 2012 Wiley Periodicals, Inc. (Less)

Published
2011
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