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Machadagrion garbei (Santos, 1961) was only known by the male holotype from Vila Nova, Bahia state, Brazil, and one male from Junqueira, Alagoas state, Brazil, collected in 1963. Over 50 years of its original description we report its rediscovery with specimens collected in Barra do Jacuípe, Camaçari County, Bahia state, Brazil, and Serra de Itabaiana, Sergipe state, Brazil. We provide illustrations and diagnosis from both sexes, including the description of the female.

The Brazilian fauna of Lestidae contains two genera (Archilestes Selys, 1862 and Lestes Leach in Brewster, 1815) with 14 species, many of which are poorly defined and/or known only by primary literature. To improve the knowledge of the Brazilian species of the genus Lestes we examined 97 specimens pertaining to 11 of the 13 described species. Additionally, a new species is described here in honor to Prof. Dr. Paulo De Marco Júnior: Lestes demarcoi (Holotype and Allotype: Brazil: Amazonas, Manaus, Reserva Adolpho Ducke, Acará trail, 02º55’46” S & 59º58’22” W, 62 m, 13.iv.2009, collected in tandem, U.G. Neiss leg. and deposited in FAAL). Diagnostic illustrations of all species are provided. Color photographs of live individuals of Lestes dichrostigma Calvert, 1909, Lestes forficula Rambur, 1842 and Lestes paulistus Calvert, 1909 are also presented.

O trabalho teve como objetivo elaborar um jogo didático de morfologia vegetal. Para elaboração do jogo, foram utilizados materiais de fácil acesso e de baixo custo. A proposta pedagógica foi exposta em um evento, no qual os participantes observaram o jogo e realizaram considerações sobre o mesmo, como poder ser aplicado em qualquer modalidade de ensino e não somente para Educação do campo e que os próprios educandos podem elaborar esse material didático. Por fim, ressalta-se a necessidade do educador relacionar o conteúdo teórico com o contexto do educando, promovendo a valorização de suas origens e o sentimento de pertencimento.

O trabalho trata-se de uma proposta de atividade investigativa sobre o tema Botânica para as aulas de Biologia do ensino médio, tendo como objetivo avaliar o potencial alelopático do extrato aquoso de Eucalyptus sobre a germinação de sementes de hortaliças (alface, rúcula, rabanete e couve), por meio de uma atividade experimental investigativa. Apresenta metodologia detalhada e informações sobre como explorar a atividade numa abordagem investigativa.

Machadagrion garbei (Santos, 1961) (Figures 1−3) Leptagrion garbei Santos 1961: 360, Figures 5–8 (Description of male, illustrations of appendages in lateral and dorsal views, pterostigma of fore wing, male prothorax in dorsal view); Santos 1979: 170 (compared with L. bocainense Santos, 1979); Davies & Tobin 1984: 82 (data on publication and distribution); Machado & Costa 1990: 301 (data on publication and distribution); Bridges 1994: (VII) 96 (data on publication, type depository, type locality and references); Costa & Mascarenhas 1998: 6 (data on types); Costa & Garrison 2001: 383, Figures: 11a, 11c, 32b (key to Brazilian species of Leptagrion, illustration of appendages in lateral and dorsal views, male forewing pterostigma); Lencioni 2006: 148, Figures 89A–89D (data on description, type locality, type deposition, distribution, and larval description, illustration of appendages in lateral and dorsal views, male prothorax in dorsal view, male hind wing pterostigma); Lencioni 2022: 90−91 (transference from Leptagrion Selys to Machadagrion Lencioni, generic characterization, distribution). Material examined. 9♂♂ and 1♀. 2♂♂ Brazil, Bahia, Camaçari, Barra do Jacuípe, Condomínio Planeta Água, (12.7205 S, 38.1386 W), 31.vii.2006, Freire, L.S. and Ferreira, P.A. leg. (FAAL); 1♂, same data but 11.ii.2007; 6♂♂ and 1♀ (in tandem) Brazil, Sergipe, Areia Branca, Serra de Itabaiana National Park, (10.7517 S, 37.3415 W, 179 m asl), 3.ii.2021, J.C. Santos leg. (UFS). Morphology of the female. Head (Figure 1a). Labium, labrum, and postclypeus blue; base of mandibles and genae pale colored, apex of mandibles black; antefrons and postfrons dark brown; remainder of head dark colored, except for two quadrangular pale postocular spots. Rear of head pale. Thorax (Figures 1a, 2 a−b). Anterior lobe of prothorax light blue dorsally, dark blue laterally with pale apical areas; medial lobe brown dorsally, light blue laterally; posterior lobe light blue laterally, brown medially, widest at base with slight lateral projections, slight medial concavity, and roughly trapezoidal shape. Medial portion of mesepisternum brown, remainder with a blue stripe extending up to the mesopleural suture; mesinfraepisternum light brown, with a blue basal coloration; mesepimeron mostly orange, bluish basally; remainder of pterothorax pale colored; legs mostly pale. Wings (Figure 1a). Hyaline; Pt light brown, quadrangular, with pale borders, overlying the space of one cell; 10 Px in FW and HW; RP1 originates at Px 7 in all wings. Abdomen (Figures 1a, 2c). S1−2 dark brown dorsally, pale blue laterally and ventrally; S2 with an apical dark brown ring; S3−6 mostly brown with pale basal and apical rings; S7 dark brown; S8 blue dorsally, dark brown laterally; S9 with a blue dorsal posterior spot, remainder brown; S10 blue dorsally, brown laterally; appendages brown with black apex; genital valves brown, styli black. Measurements. FW: 20.5; HW: 19.5; AL: 38.9; TL: 46.7. Differential diagnosis. Unfortunately, we could not perform a comparison of our specimens of M. garbei with the type material, as it was lost in the big fire of 2018 at the National Museum of the Federal University of Rio de Janeiro. However, we had access to a bona fide specimen of this taxon, housed at FAAL, collected in 2006 and compared with the original series. Machadagrion garbei (Figures 1 a−b) is a species morphologically close to M. cyanostigma (Machado, 2012) and M. dardanoi (Santos, 1968) according to male hind lobe of prothorax and cercus (Figures 3 a−d) and female hind lobe of prothorax (Figures 2 a−b). In M. garbei, the ventral “C”-shaped branch (Fig. 3c) is subequal to dorsal branch (longer than dorsal branch in M. dardanoi see Lencioni 2022); the dorsal branch of cercus in M. garbei (Fig. 3c) is similar to M. cyanostigma, but is truncated in the latter (see Lencioni 2022). Additionally, M. cyanostigma is the only species with a blue pterostigma (Machado 2012), thus it can be easily separated from other congeners. The female of M. garbei differs from those Machadagrion females by the following combination of characters (other species on parenthesis): abdomen length> 38 mm (22 mm in M. dardanoi; 31 mm in M. cyanostigma), hind lobe of prothorax (Figures 2 a−b) widest at base, with slight lateral projections, slight medial concavity, and roughly trapezoidal shape (narrower at base on M. dardanoi; roughly trapezoidal, no medial concavity, and without lateral projections on M. cyanostigma). Final remarks. The IUCN red list assessors had classified M. garbei as Data Deficient, and stated: “This poorly known species was collected for the last time in 1963 (Costa & Garrison 2001). Nothing else is known about distribution and ecology” (Vilela & Guillermo-Ferreira 2021). Unfortunately, the lack of distributional data for M. garbei is not an exception for Odonata species, as it is the case for 28% of all the assessed taxa (IUCN 2021). Until now, M. garbei was known to occur in the states of Bahia and Alagoas (Costa & Garrison 2001), and in the present study we expand its record to Sergipe state, which is located between the two states mentioned before (Figure 4). Even if the distribution of M. garbei falls within the expected range of occurrence of the species, our discovery proves that the species is still thriving on their natural habitats, including a protected area. Thus, we expect that the rediscovery of this poorly known species to be an essential step to establish strategies for its protection., Published as part of Vilela, Diogo Silva, Lencioni, Frederico Augusto De Atayde, Furieri, Karina Schmidt & Santos, Jean Carlos, 2022, The rediscovery of Machadagrion garbei (Santos, 1961) (Odonata: Coenagrionidae) with notes on the hitherto unknown female, pp. 391-396 in Zootaxa 5124 (3) on pages 392-394, DOI: 10.11646/zootaxa.5124.3.8, http://zenodo.org/record/6411030, {"references":["Santos, N. D. (1961) Duas novas especies do genero \" Leptagrion \" Selys, 1876. (Odonata, Coenagrionidae). Revista Brasileira de Biologia, 21 (4), 359 - 362.","Santos, N. D. (1979) Descricao de Leptagrion bocainense Santos, 1978 cenagrionideo bromelicola (Odonata: Coenagrionidae). Anais da Sociedade de Entomologia do Brasil, 8 (1), 167 - 173. https: // doi. org / 10.37486 / 0301 - 8059. v 8 i 1.179","Davies, D. A. L. & Tobin, P. (1984) The dragonflies of the world: a systematic list of the extant species of Odonata. Volume 1. Zygoptera, Anisozygoptera. Societas Internationalis Odonatologica Rapid Communications, Supplement 3, i - ix + 1 - 127.","Machado, A. B. M. & Costa, J. M. (1990) Obituary of Newton Dias dos Santos. Odonatologica, 19 (3), 297 - 308.","Bridges, C. A. (1994) Catalogue of the family - group, genus - group and species - group names of the Odonata of the world. 3 rd Edition. C. A. Bridges, Urbana, Illinois, xlvi + 905 pp. https: // doi. org / 10.5962 / bhl. title. 15291","Costa, J. M. & Mascarenhas, B. J. A. (1998) Catalogo do material-tipo de Odonata (Insecta) do Museu Nacional. Publicacoes Avulsas do Museu Nacional, 76, 1 - 30.","Costa, J. M. & Garrison, R. W. (2001) Description of the female of Leptagrion aculeatum Santos, 1965 with keys to the known species (Zygoptera: Coenagrionidae). Odonatologica, 30 (4), 381 - 394.","Lencioni, F. A. A. (2006) Damselflies of Brazil - An illustrated identification guide. II. - Coenagrionidae. All Print Editora, Sao Paulo, viii + 419 pp.","Lencioni, F. A. A. (2022) Analysis of male Leptagrion Selys, 1876 sensu lato (Odonata: Coenagrionidae) with description of four new genera and a new species. Zootaxa, 5105 (1), 63 - 104. https: // doi. org / 10.11646 / zootaxa. 5105.1.3","Machado, A. B. M. (2012) Leptagrion cyanostigma sp. nov. from Brazil with a study of blue pterostigma in Zygoptera (Odonata: Coenagrionidae). International Journal of Odonatology, 15 (2), 81 - 86. https: // doi. org / 10.1080 / 13887890.2012.685837","Vilela, D. S. & Guillermo-Ferreira, R. (2021) Leptagrion garbei. The IUCN Red List of Threatened Species, 2021, e. T 139153035 A 145942988. https: // doi. org / 10.2305 / IUCN. UK. 2021 - 2. RLTS. T 139153035 A 145942988. en","IUCN (2021) The IUCN Red List of Threatened Species, Version 2021 - 2. Available from: https: // www. iucnredlist. org (accessed 3 December 2021)"]}

Made available in DSpace on 2022-04-28T19:48:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-16 A new species of Leptagrion Selys, 1876 is described and named in honor to Dr. Jerome Constant: Leptagrion jeromei (Holotype: Brazil: Bahia, Jussari, RPPN Serra do Teimoso, Jequitiba tree (Cariniana legalis (Mart.) Kuntze, 1898) at 32 m, 10.v.2005, K.S. Furieri leg and deposited in FAAL). This species was confused for a long time with L. andromache Hagen in Selys, 1876 its closest congener. Diagnostic illustrations of L. andromache (lectotype and specimens collected at AmapBrazil) and L. jeromei spec. nov. are presented. We also present the northernmost record for L. andromache in Amap state, Northern Brazil. Laboratrio de Biologia Aqutica Universidade Estadual Paulista So Paulo, Departamento de Cincias Biolgicas ,Faculdade de Cincias e Letras de Assis Programa de Ps-Graduao em Biodiversidade Tropical AP, Universidade Federal do Amap Laboratrio de Ensino de Zoologia CEUNES UFES BR 101 Norte, Departamento de Cincias Agrrias e BiolgicasKm 60 Laboratrio de Biologia Aqutica Universidade Estadual Paulista So Paulo, Departamento de Cincias Biolgicas ,Faculdade de Cincias e Letras de Assis

One of the greatest obstacles to biodiversity conservation is the lack of information to prioritize efforts on practical actions. This lack of information comes mainly from absence of inventories in many areas where few groups have reasonably complete databases. The distribution of the dragonfly fauna of Brazil is little known - only 29% of the Brazilian territory presents data about the richness of Odonata. In this contribution, a database of Odonata species of Santa Teresa Municipality was built, Espírito Santo State, Brazil. The database has 627 specimens registered, 77 species distributed in eight families and thirty-eight genera, forty of these species are common, one endemic of the State of Espírito Santo, and nineteen endemic of Brazil. In relation to the measure of conservation, four species are present in the category Insufficient Data (DD), one present in the Nearly Endangered (NT) category, three species in the Vulnerable category (VU) and one in the category In Danger (EN). Twenty-five species are considered new occurrences for the city. These results suggest that the fauna of the studied region deserves attention and points to the interest in implanting future conservation units in the region of Santa Teresa municipality.

Objetivo: comparar a avaliação antropométrica e fatores motivacionais de praticantes de hidroginástica e caminhada. Método: pesquisa quase experimental, sem grupo controle e de cunho quantitativo, realizada em Guarapuava, Paraná. Analisou-se o IMC, porcentagem de gordura, circunferências, relação cintura quadril, teste de força com preensão manual e teste de flexibilidade com o banco de wells. Aplicou-se também, o questionário de motivação (IMPRAF-54). Após a coleta de dados, foi realizada a análise estatística através do teste T, comparando as medidas entre os grupos. Resultados: foi observado que a motivação mais prevalente para praticar as atividades físicas foi o fator saúde. Na avaliação antropométrica, as praticantes de hidroginástica possuíam índices superiores. Nos testes de força e flexibilidade as praticantes de hidroginástica apresentaram maior força em membros superiores, enquanto que as praticantes de caminhada maios flexibilidade em membros inferiores. Conclusão: a hidroginástica possui benefícios extras em comparação à caminhada e a motivação das participantes para praticar essas atividades físicas indica sua preocupação com a condição e qualidade de vida.

In this paper, we provide taxonomic updates on the genus Leptagrion Selys: the females of L. acutum Santos and L. porrectum Selys are illustrated and diagnosed for the first time. We also add to the original description of L. acutum based on a male collected in the Atlantic Forest. A key for females is also provided for the currently known species of Southeast of Brazil.

Background Preoperative anemia and transfusion are associated with increased morbidity and mortality in cardiac surgery patients. It is unclear which of these factors plays the leading role in poor outcomes after cardiac surgery. The goal of this study was to analyze the influence of anemias of varying severity and intraoperative transfusion on long-term survival, and to characterize their interaction in cardiac surgery patients. Methods This was an observational cohort study conducted at a German university hospital. All patients undergoing cardiac surgery between 2006 and 2011 were screened for eligibility; duration of follow-up was 3 years. A total of 4494 patients were suitable for analysis; data on long-term survival were available for 3131 of these patients. The main outcome measure was survival at the 3-year follow-up. Length of stay and in-hospital mortality were assessed as secondary outcomes. Results Multivariate Cox regression analyses indicated that both the severity of preoperative anemia (mild anemia: hazard ratio [HR], 1.441; 95% confidence interval [CI], 1.201-1.728; severe anemia: HR, 1.805; 95% CI, 1.336-2.440) and intraoperative transfusion (HR, 1.340; 95% CI, 1.109-1.620) were associated with decreased long-term survival. Long-term survival was worse in anemic patients who received an intraoperative transfusion compared with those who did not receive an intraoperative transfusion. Conclusions Both preoperative anemia and transfusion are by themselves and in combination associated with decreased long-term survival. When anemic patients require transfusion, our results provide evidence that the risk of death after cardiac surgery may depend to a considerable extent on the severity of preoperative anemia.

The prevalence of gastric diseases is increasing with H. pylori, the causative agent of acute and chronic gastritis, being a major predisposing factor for peptic ulcer disease and gastric carcinoma. C. jejuni is the most common cause of enteric infections, particularly among children, resulting in severe diarrhoea. Increasing drug resistance of these bacteria against standard antibiotics, and the more widespread use of herbal medicines, favours investigations into additional anti-Helicobacter and anti-Campylobacter effects of phytotherapeutics that are already used for their beneficial effects on bowel and digestive functions. Twenty-one hydroethanol herbal extracts and four essential oils were screened for antibacterial activity using a modification of a previously described micro-dilution assay and compared with the inhibitory effects of antibiotics. The herbal extracts showing the highest growth inhibition of C. jejuni were Calendula officinalis, Matricaria recutita, Zingiber officinale, Salvia officinalis, Foeniculum vulgare and Silybum marianum. Agrimonia eupatoria, Hydrastis canadensis, Filipendula ulmaria and Salvia officinalis were the most active herbal extracts in inhibiting the growth of H. pylori. This study provides evidence for additional beneficial effects of phytotherapeutics marketed for their gastrointestinal effects and identifies new beneficial antibacterial effects for some herbal medicines not currently recommended for gastrointestinal problems.

Prevalence of antibody reactive with Sin Nombre hantavirus (SNV) was evaluated from rodents captured over 31 months (March 1988 to September 1990) from six mark-recapture grids on the central Argentine Pampa. The most frequently infected rodents were: Akodon azarae (31/459), Necromys benefactus (8/141), and Oligoryzomys flavescens (10/281), which are known hosts of Pergamino, Maciel, and Lechiguanas hantaviruses, respectively. Relative population density and antibody prevalence varied seasonally and from year to year, population densities were highest in fall and prevalences were highest in spring. A positive association between antibody prevalence and body weight corroborated findings from other studies suggesting that hantaviruses are maintained in reservoir populations by horizontal transmission. In two of three host species, transmission was more frequent among male than among female mice. We found no evidence for a detrimental effect of hantavirus infection on host body weight, growth, longevity, movement, or reproductive preparedness. This analysis, based on cryopreserved specimens, represents the earliest conducted longitudinal, mark-recapture study of the dynamics of infection of autochthonous American hantaviruses in their sigmodontine host populations.

A identidade de marca é um dos componentes centrais do processo de gestão de marcas (branding) das organizações e as estratégias de marketing sensorial podem contribuir para a construção da identidade de marca, seja por meio do uso de estímulos sensoriais na comunicação da marca, no ponto de venda e no relacionamento com o cliente. Assim, o presente estudo analisa como estratégias de marketing sensorial contribuem para a construção da identidade de marca segundo o modelo teórico de Aaker (2007) em uma organização do setor de móveis planejados. Para tanto, o estudo envolveu a aplicação de pesquisa exploratória, de abordagem qualitativa e na forma de estudo de caso. A coleta de dados foi realizada por meio de levantamento documental, entrevista em profundidade (semi-estruturada) e observação direta com gestores da empresa, lojistas e clientes. Os dados coletados foram analisados pelo método análise de conteúdo com abordagem qualitativa e categorização temática de dados. Os resultados do estudo apontam que a empresa utiliza os cinco sentidos humanos como estratégia de marketing, principalmente estímulos sensoriais de natureza visual (decoração, seleção de cores e ambientação de lojas), olfativa (essência desenvolvida especialmente para a marca) e tátil (texturas diferentes em brindes e produtos); que as estratégias sensoriais são percebidas como relevante pelos gestores na construção da identidade da marca e que os benefícios mais apontados são satisfação de clientes, lealdade à marca e indicação de novos clientes The brand identity is one of the central components of the brand management process (branding) of organizations and sensory marketing strategies can contribute to building the brand identity, either through the use of sensory stimuli in brand communication in point of sale and customer relationships. Thus, this study analyzes how sensory marketing strategies contribute to building the brand identity according to the theoretical model of Aaker (2007) in an organization's customized furniture industry. Therefore, the study involved the application of exploratory research, qualitative approach and in the form of case study. Data collection was performed through documentary survey, in-depth interviews (semi-structured) and direct observation with company managers, shopkeepers and customers. Data were analyzed by using content analysis with qualitative approach and thematic categorization of data. The study results indicate that the company uses the five human senses as a marketing strategy, especially sensory stimuli visual nature (decor, color selection and ambiance stores), olfactory (essence developed especially for the brand) and tactile (different textures in gifts and products); that sensory strategies are perceived as important by managers in building brand identity and the most mentioned benefits are customer satisfaction, brand loyalty and appointment of new customers

Hantavirus activity in 39 National Parks in the eastern and central United States was surveyed by testing 1,815 small mammals of 38 species for antibody reactive to Sin Nombre virus. Antibody-positive rodents were found throughout the area sampled, and in most biotic communities. Antibody was detected in 7% of 647 deer mice (Peromyscus maniculatus), 2% of 590 white-footed mice (P. leucopus), 17% of 12 rice rats (Oryzomys palustris), 3% of 31 cotton rats (Sigmodon hispidus), and 33% of 18 western harvest mice (Reithrodontomys megalotis). Antibody was also found in three of six species of voles, and in one of 33 chipmunks (Tamias minimus). Prevalence among Peromyscus was highest in the northeast. Although few cases of hantavirus pulmonary syndrome have been identified from the eastern and central regions, widespread infection in reservoir populations indicates that potential exists for human infection throughout much of the United States.

Ao longo da dissertação, para dar forma e legitimidade à intuição e à experiência emocional e intelectiva que a autora teve com a obra do psicanalista brasileiro Gilberto Safra, foi empreendido o esforço de fabricar uma interpretação que acontecesse como reposta a esta obra. Para tanto, propusemos uma leitura pormenorizada (o que inclui a leitura sistemática e a leitura próxima desconstrutiva) de dois livros de Safra: Momentos Mutativos em Psicanálise, sua tese de doutorado e A Face Estética do Self, sua tese de livre docência. Este recorte da obra se ancorou na hipótese de que haveria algo na construção do pensamento psicanalítico de Safra que se modificou consideravelmente entre estes dois momentos de sua trajetória pessoal, autoral e na sua formação como analista. Em resposta a estas questões, uma leitura entrelaçada das teses que compõem seu pensamento psicanalítico com suas condições de possibilidade nos levou a compreender que a escrita de Safra deixa entrever o tortuoso movimento de busca de um analista por sua morada conceitual, ética e estética. O que está em jogo é sempre o processo de singularização: do analista, do paciente e das possibilidades de se fazer teoria e clínica psicanalíticas nos tempos atuais This dissertation, in order to shape and legitimize the authors intuition, and emotional and intellectual experience with the works of Brazilian psychoanalyst Gilberto Safra, strived to create an interpretation that could stand as answer to said works. As such, we proposed a detailed reading (which includes a systematic and then deconstructive reading) of two of Safras books: Mutative Moments in Psychoanalysis, his doctoral thesis, and The Aesthetic Face of the Self, his associate professorship thesis. This partial selection of his work is anchored on the hypothesis that there would be something in the construction of Safras psychoanalytical thinking which had been considerably altered between these two points in his personal and authorial trajectory, and his training as an analyst. In response to these questions, an interwoven reading of these theses which make up his psychoanalytical thought, with their conditions of possibility, led us to understand that Safras writing allows us to glimpse an analysts tortuous path in search of his conceptual, ethical and aesthetic references. Whats at stake is always the singularization process: of the analyst, the patient and the possibilities of creating psychoanalytical theory and doing psychoanalytical clinic work in current times

Conselho Nacional de Desenvolvimento Científico e Tecnológico The Leptagrion genus is strictly associated to bromeliads in many different ecosystems in South America showing a high diversity in the Atlantic Forest. There are two species of this genus that are threatened of extinction in Brazil (The Brazilian red list and the red list of the state of Espírito Santo), but there are others that we do not have enough information to determine a clear status of conservation. The loss of the habitat is probably the main threat to the species that live in the Atlantic Forest surroundings that was reduced to 5% of its original distribution. Leptagrion acutum is probably the most threatened species of this genus. It was rediscovered in the Reserva Biológica de Córrego Grande , the only known registered area at the present time, despite of the extensive search in the region. Leptagrion andromache is a new registry in Bahia, L. dispar is a new registry for Espírito Santo and the distribution of L. capixabae e L. garbei is extended. Bromeliagrion and Leptagrion form a monophyletic group, but the low support in most of the branches does not allow a strong conclusion about most of the phylogenetic relations within this group. The actions for a conservation plan of these species in the Atlantic Forest should include an integrated management of the units of conservation of the region and an extension of fire protection that has caused severe loss of habitat in the distribution area of these species. In addition, the handling of bromeliads is a viable strategy to the preservation of Leptagrion species and may be realized by translocation of some bromeliads or cultivation by using the local plant population. The Corredor Central da Mata Atlântica has as role of great importance in the preservation of species of this genus for sheltering the biggest phylogenetic diversity, the biggest Leptagrion diversity species and most of the Leptagrion species threatened. O gênero Leptagrion é, estritamente, associado a bromélias em muitos ecossistemas diferentes na América do Sul, apresentando alta diversidade na Floresta Atlântica. Existem duas espécies dessas libélulas ameaçadas de extinção no Brasil (Lista do IBAMA e Lista Vermelha do Espírito Santo), mas há outras com informações insuficientes para uma determinação clara de seu status de conservação. A perda de habitat é, provavelmente, a principal ameaça para as espécies que habitam o Domínio da Mata Atlântica, que foi reduzida a 5% de sua distribuição original. Leptagrion acutum, provavelmente, a espécie mais ameaçada do gênero, foi redescoberta na Reserva Biológica de Córrego Grande, única área de registro conhecida, apesar da extensiva busca na região. Leptagrion andromache é um novo registro para a Bahia, L. dispar é um novo registro para o Espírito Santo e a distribuição de L. capixabae e L. garbei é ampliada. Bromeliagrion e Leptagrion formam um grupo monofilético, mas o baixo suporte da maioria dos ramos não permite uma conclusão forte sobre a maioria das relações filogenéticas dentro desse grupo. As ações para um plano de conservação dessas espécies na região da Floresta Atlântica devem incluir um manejo integrado das unidades de conservação da região e uma ampliação da proteção contra o fogo, que causou severas perdas de habitat na área de distribuição dessas espécies. Além disso, o manejo de bromélias é uma estratégia viável à preservação das espécies de Leptagrion e pode ser realizado por translocação de algumas rosetas ou por cultivo de bromélias usando matrizes da população local. O Corredor Central da Mata Atlântica tem papel de grande importância na preservação das espécies desse gênero por abrigar a maior diversidade filogenética e de espécies de Leptagrion, além da maioria das espécies de Leptagrion ameaçadas de extinção.

In vitro experiments were performed on human bladder carcinoma cells to evaluate the efficiency of photodynamic activity of protoporphyrindisodiumsalt encapsulated within liposomes. The bladder carcinoma cell line EJ 28, (Tumorba-nk Heidelberg) was grown on DMEM + 10% FCS + 2% Glutamine + 1% Penicillin/Streptomycin as a monolayer culture. In the log phase, cells were trypsinized, counted and inoculated into 35 mm-diameter multiwells at 105 cells/well and allowed to grow for 24 h. The cells were incubated for 1 h with 5, 10, 20 (mu) g/ml protoporphyrindisodiumsalt in liposomes. (DPPC/Cholesterol 7:3). After incubation cells were refed with complete medium and irradiated with 3 and 6 J/cm2. After irradiation, the cells were incubated for 2 days at 37 degree(s)C, then fixed, stained, counted and compared to the control group. Mean survival rates of 7.21%, 2.99%, 1.33% after irradiation with 3 J/cm2 and 4.3%, 1.48% and 0.88% after irradiation with 6 J/cm2 were found. By using a fluorescence microscope, we evaluated the intracellular uptake of protoporphyrindisodiumsalt in liposomes. The vesicular fluorescence pattern exhibited a concentration of photosensitizer in the nuclear membrane and adjacent parts of the cytoplasm as well as in the plasma membrane. By transmission electron microscopy marked changes were observed at the mitochondria with dissolution of the cristae and development of vacuols.© (1995) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.

The purpose of this study was to determine whether phospholipid liposomes that are bound by the human bladder carcinoma cells can improve the delivery of the photosensitizer and the effectiveness of photodynamic therapy.

Paragryllini Desutter is a Neotropical tribe of crickets with species distributed from southern Mexico to Brazil. Currently are known 24 species assigned to six genera: Benoistella Uvarov, 1939; Mexiacla Gorochov, 2007; Oaxacla Gorochov, 2007; Paragryllus Guérin- Méneville, 1844; Rumea Desutter, 1988; and Silvastella Desutter- Grandcolas, 1992. In this paper new taxa from the Brazilian Central Amazonia are described. The genus Rumea is reported for the first time from this region, represented by three new species from the Central Amazonian: R. zebra sp. nov., R. manauensis sp. nov. and R. tigris sp. nov. Also two new genera are described: Marciela gen. nov., including M. longipes sp. nov. from Brazil and M. crybelos (Nischk & Otte, 2000) comb. nov. from Ecuador; and Sperberus gen. nov. including only S. arboricolus sp. nov. The morphology of the male genitalia of the Rumea species is discussed and a new interpretation is proposed. For the illustration of the genitalia of males and females a photographic technique was used: the structure was immersed in glycerin and photographs were taken on different levels of focous. For the construction of the final images the free software CombineZM was used. The biogeography of the Paragryllini is discussed based on the known distribution of the species. This study contributes to the knowledge of the group, increases its geographical distribution, and may serve as a starting point for future research on the Paragryllini in Brazil. Paragryllini Desutter é uma tribo Neotropical com espécies distribuídas do sul do México até o Brasil. Atualmente são conhecidas 24 espécies subordinadas a 6 gêneros: Benoistella Uvarov, 1939; Mexiacla Gorochov, 2007; Oaxacla Gorochov, 2007; Paragryllus Guérin-Méneville, 1844; Rumea Desutter, 1988; e Silvastella Desutter-Grandcolas, 1992. Nesta dissertação são descritos novos táxons de Paragryllini da Amazônia Central brasileira. Faz-se o primeiro registro do gênero Rumea para esta área, com a descrição de três espécies novas: R. zebra sp. nov., R. manauensis sp. nov. e R. tigris sp. nov.. Também são descritos dois gêneros novos: Marciela gen. nov., que inclui Marciela longipes sp. Nov. do Brasil e Marciela crybelos (Nischk & Otte, 2000) comb. nov. do Equador; e Sperberus gen. nov. incluindo unicamente Sperberus arboricola sp. nov. Para as espécies do gênero Rumea, discutem-se as características morfológicas da genitália dos machos e se propõe uma nova interpretação da dessa estrutura. Para ilustrar a genitália tanto dos machos quanto das fêmeas, desenvolveu-se uma técnica de fotografia: a estrutura era inmersa em glicerina e eran tiradas fotografias em diferentes níveis de foco. Para a construção da fotografia final usou-se o software livre CombineZM. Discutem-se alguns aspectos biogeográficos baseados na distribuição conhecida das espécies de Paragryllini. Este estudo proporciona um aporte para o conhecimento do grupo, já que se amplia sua dibribuiçao geográfica, e puede servir como um punto de partida para futuras pesquiças sobre os Paragryllini no Brasil.