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Aim: To investigate the effect of nerve growth factor (NGF) neutralization on Na + channel plasticity of bladder afferent neurons in mice with spinal cord injury (SCI)., Main Methods: Female C57/BL6 mice were randomly divided into spinal intact (SI) group, SCI group and SCI + NGF-Ab group. SCI was induced by spinal cord transection at the Th8/9 level. In SCI + NGF-Ab group, anti-NGF antibodies (10 μg·kg-1 per hour) were continuously administered for 2 weeks using osmotic pumps. Bladder afferent neurons were labelled with Fluoro‑gold (FG) injected into the bladder wall. L6-S1 dorsal root ganglion (DRG) neurons were dissociated and whole-cell patch clamp recordings were performed on FG-labelled neurons. Expression of Nav1.7 and Nav1.8 was examined by immunofluorescent staining., Key Findings: Whole-cell patch clamp recordings showed that TTX only partially inhibited action potentials (AP) and Na + currents of bladder afferent neurons in SI mice, but it almost completely inhibited them in SCI mice. Total and TTX-sensitive Na + currents were increased and TTX-resistant currents were decreased in bladder afferent neurons from SCI mice vs. SI mice. These changes in SCI mice were significantly reversed by NGF-antibody treatment. Immunostaining results showed the increased and decreased levels of Nav1.7 and Nav1.8, respectively, in FG-labelled bladder afferent neurons in SCI mice vs. SI mice, which was significantly reversed in SCI + NGF-Ab mice., Significance: NGF mediates the Na + channel plasticity with a shift from TTX-resistant Nav1.8 to TTX-sensitive Nav1.7 in bladder afferent neurons, which could be a possible underlying mechanism of bladder afferent hyperexcitability and detrusor overactivity after SCI., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Coordination between the urinary bladder (BL) and external urethral sphincter (EUS) is necessary for storage and elimination of urine. In rats interneuronal circuits at two levels of the spinal cord (i.e., L6-S1 and L3-L4) play an important role in this coordination. In the present experiments retrograde trans-synaptic transport of pseudorabies virus (PRV) encoding fluorescent markers (GFP and RFP) was used to trace these circuits. To examine the relative localization of EUS-related and BL-related interneuronal populations we injected PRV-GFP into the EUS and PRV-RFP into the BL wall. The PRV infected populations of spinal interneurons were localized primarily in the dorsal commissure (DCM) of L6/S1 and in a hypothesized lumbar spinal coordinating center (LSCC) in L3/L4 above and lateral to central canal (CC). At both sites colocalization of markers occurred in a substantial number of labeled interneurons indicating concomitant involvement of these double-labelled neurons in the EUS- and BL-circuits and suggesting their role in EUS-BL coordination. Intense GFP or RFP fluorescent was detected in a subpopulation of cells at both sites suggesting that they were infected earlier and therefore likely to represent first order, primary interneurons that directly synapse with output neurons. Larger numbers of weakly fluorescent neurons that likely represent second order interneurons were also identified. Within the population of EUS-related first order interneurons only 3-8 % exhibited positive immunoreaction for an early transcription factor Pax2 specific to GABAergic and glycinergic inhibitory neurons suggesting that the majority of interneurons in DCM and LSCC projecting directly to the EUS motoneurons are excitatory., (© 2020 The Author(s).)

Coordination of activity of external urethral sphincter (EUS) striated muscle and bladder (BL) smooth muscle is essential for efficient voiding. In this study we examined the morphological and electrophysiological properties of neurons in the L3/L4 spinal cord (SC) that are likely to have an important role in EUS-BL coordination in rats. EUS-related SC neurons were identified by retrograde transsynaptic tracing following injection of pseudorabies virus (PRV) co-expressing fluorescent markers into the EUS of P18-P20 male rats. Tracing revealed not only EUS motoneurons in L6/S1 but also interneurons in lamina X of the L6/S1 and L3/L4 SC. Physiological properties of fluorescently labeled neurons were assessed during whole-cell recordings in SC slices followed by reconstruction of biocytin-filled neurons. Reconstructions of neuronal processes from transverse or longitudinal slices showed that some L3/L4 neurons have axons projecting toward and into the ventro-medial funiculus (VMf) where axons extended caudally. Other neurons had axons projecting within laminae X and VII. Dendrites of L3/L4 neurons were distributed within laminae X and VII. The majority of L3/L4 neurons exhibited tonic firing in response to depolarizing currents. In transverse slices focal electrical stimulation (FES) in the VMf or in laminae X and VII elicited antidromic axonal spikes and/or excitatory synaptic responses in L3/L4 neurons; while in longitudinal slices FES elicited excitatory synaptic inputs from sites up to 400 μm along the central canal. Inhibitory inputs were rarely observed. These data suggest that L3/L4 EUS-related circuitry consists of at least two neuronal populations: segmental interneurons and propriospinal neurons projecting to L6/S1., (Copyright © 2019 IBRO. Published by Elsevier Ltd. All rights reserved.)

There has been a recent resurgence of interest in therapeutic modalities using transcranial weak electrical stimulation through scalp electrodes, such as trans-cranial direct current stimulation (tDCS), as a means of experimentally modifying and studying brain function and possibly treating psychiatric conditions. A range of electrotherapy paradigms have been investigated, but no consistent method has been indicated for reporting reproducible stimulation "dosage." Anecdotal reports, case studies, and limited clinical trials with small numbers suggest that tDCS may be effective in treating some patients with depression, but methods for selecting the optimal stimulation parameters ("dosage") are not clear, and there is no conclusive indication that tDCS is an effective treatment for depression. Larger, controlled studies are necessary to determine its safety and efficacy in a clinical setting. If tDCS can be established as an effective treatment for depression, it would represent a particularly attractive electrotherapy option, as it is a relatively benign and affordable treatment modality. An accurate system for describing reproducible treatment parameters is essential so that further studies can yield evidence-based guidelines for the clinical use of transcranial current stimulation. Development of appropriate parameters requires a biophysical understanding of how electrotherapy affects brain function and should include different paradigms for different clinical applications. As with any dosage guidelines, such a system does not supersede physician judgment on safety.

Field potentials recorded in the olfactory bulb glomerular layer (GL) are thought to result mainly from activation of mitral and tufted cells. The contribution of juxtaglomerular cells (JG) is unknown. We tested the hypothesis that JG are the main driving force to novel spontaneous glomerular layer field potentials (sGLFPs), which were recorded in rat olfactory bulb slices maintained in an interface chamber. We found that sGLFPs have comparable magnitudes, durations and frequencies both in standard horizontal slices, where all layers with all cell types were present, and in isolated GL slices, where only JG cells were preserved. Hence, the impact of mitral and deep/medium tufted cells to sGLFPs turned out to be minor. Therefore, we propose that the main generators of sGLFPs are JG neurons. We further explored the mechanism of generation of sGLFPs using a neuronal ensemble model comprising all types of cells associated with a single glomerulus. Random orientation and homogenous distribution of dendrites in the glomerular neuropil along with surrounding shell of cell bodies of JG neurons resulted in substantial spatial restriction of the generated field potential. The model predicts that less than 20% of sGLFP can spread from one glomerulus to an adjacent one. The contribution of JG cells to the total field in the center of the glomerulus is estimated as approximately 50% ( approximately 34% periglomerular and approximately 16% external tufted cells), whereas deep/medium tufted cells provide approximately 39% and mitral cells only approximately 10%. Occasionally, some sGLFPs recorded in adjacent or remote glomeruli were cross-correlated, suggesting involvement of interglomerular communication in information coding. These results demonstrate a leading role of JG cells in activation of the main olfactory bulb (MOB) functional modules. Finally, we hypothesize that the GL is not a set of independent modules, but it represents a subsystem in the MOB network, which can perform initial processing of odors.

Luminescence and absorption stains specific for DNA (acridine orange, ethidium bromide), proteins (silver nitrate), and lipids (Sudan III) were used to study the distribution of DNA, proteins, and lipids in sections of the olfactory bulb in rats, studies being performed after fixation of brains with paraformaldehyde. DNA was found to be more abundant in the glomerular cell layer than the mitral cell layer. Higher quantities of DNA were present in the granular layer, located beneath the mitral layer. The characteristics of cell layers in the olfactory bulb were studied in rats aged two days and one month. There were differences between the layers of rats of different ages in terms of the content and distribution of DNA, though there were no differences in the total protein or lipid contents. Glomeruli were immature in two-day-old rats.

Theta rhythms are behaviorally relevant electrical oscillations in the mammalian brain, particularly the hippocampus. In many cases, theta oscillations are shaped by inhibitory postsynaptic potentials (IPSPs) that are driven by glutamatergic and/or cholinergic inputs. Here we show that hippocampal theta rhythm IPSPs induced in the CA1 region by muscarinic acetylcholine receptors independent of all glutamate receptors can be briefly interrupted by action potential-induced, retrograde endocannabinoid release. Theta IPSPs can be recorded in CA1 pyramidal cell somata surgically isolated from CA3, subiculum, and even from their own apical dendrites. These results suggest that perisomatic-targeting interneurons whose output is subject to inhibition by endocannabinoids are the likely source of theta IPSPs. Interneurons having these properties include the cholecystokinin-containing cells. Simultaneous recordings from pyramidal cell pairs reveal synchronous theta-frequency IPSPs in neighboring pyramidal cells, suggesting that these IPSPs may help entrain or modulate small groups of pyramidal cells.

Using luminescent and absorption stains, specific for DNA (acridine orange, ethidium bromide), proteins (silver nitrate) and lipids (sudan III), the distribution of these substances was studied in the sections of rat olfactory bulbs, fixed by paraformaldehyde. DNA prevalence was found in glomerular cell layer as compared with the mitral one. Large amount of DNA was detected in granular cell layer, underlying the mitral one. The peculiarities of cellular layers of olfactory bulbs of 2-day-old rats were compared with those ones in 1-month-old animals. In rats of different ages, the differences were found in DNA content and distribution between layers, while no differences were detected in total protein and lipids. In 2-day-old rats glomerular underdevelopment was demonstrated.

This review is concerned with neurogenesis in the mature mammalian brain with emphasis on cell population renewal in the olfactory bulb (OB). The structural and functional features of the OB are considered along with data on neurotropic viruses and toxic dust penetration into the CNS through the OB. We hypothesize a protective role of neurogenesis in the mature OB. This suggests that normal renewal of cell populations in the OB is an important barrier mechanism protecting the brain from invasion of small amounts of harmful neurotropic agents (ex. viruses and particles of toxic dust), which can cause various neurodegenerative diseases.

The hippocampal rhythms observed in vivo are the result of a complex interplay between cellular and synaptic properties within the hippocampus, and extra-hippocampal tonic as well as periodic inputs. For the stable rhythm to occur, the hippocampal circuitry should have the potential to oscillate at the specific frequencies. The in vitro studies revealed multiple mechanisms supporting the generation of the theta rhythm, which is the main operational mode of the hippocampus. In the hippocampus and related structures cellular membranes can oscillate at theta rhythm when they are depolarized to near-threshold membrane potentials; membranes are also adjusted to resonate with the external signal applied at theta frequency. Synaptically connected hippocampal network alone can generate theta rhythm when a necessary tonic excitation is provided. Finally, rhythmic inputs in theta range from the septum and entorhinal cortex have a propensity to synchronize oscillations in the whole hippocampal formation and associated structures to operate in a unified mode of activity. Based on the results obtained in slices and slice cultures, the present review shows this multilevel hierarchy, which serves to guarantee easy occurrence and reliable maintenance of the theta rhythm in the hippocampus.

Centre-surround inhibition--the suppression of activity of neighbouring cells by a central group of neurons--is a fundamental mechanism that increases contrast in patterned sensory processing. The initial stage of neural processing in olfaction occurs in olfactory bulb glomeruli, but evidence for functional interactions between glomeruli is fragmentary. Here we show that the so-called 'short axon' cells, contrary to their name, send interglomerular axons over long distances to form excitatory synapses with inhibitory periglomerular neurons up to 20-30 glomeruli away. Interglomerular excitation of these periglomerular cells potently inhibits mitral cells and forms an on-centre, off-surround circuit. This interglomerular centre-surround inhibitory network, along with the well-established mitral-granule-mitral inhibitory circuit, forms a serial, two-stage inhibitory circuit that could enhance spatiotemporal responses to odours.

The effects of acetylcholine on the spike discharges of neurons induced by iontophoretic application of excitatory amino acids to the bodies and dendrites of cells were studied in 98 neurons in living slices of guinea pig parietal cortex. Acetylcholine applied microiontophoretically to both the bodies and dendrites facilitated improvements in the parameters of responses induced by dendritic activation, with significant decreases in latent periods and increases in the intensity and duration of responses. Thee effects were stably induced at distances of 300 microm from the body and lasted 1 min after exposure to acetylcholine ended. Responses induced by application of excitatory amino acids directly to the cell body did not change significantly in the presence of acetylcholine regardless of the point on the membrane at which they were applied. It is concluded that the predominant effect of acetylcholine is on the efficiency of dendrosomatic conduction.

Acetylcholine effects on neuronal firing responses evoked by somatic or dendritic applications of excitatory amino acids were studied in slices of guinea-pig parietal cortex. Excitatory reactions initiated by dendritic activation were enhanced by acetylcholine wherever it was iontophoretically applied: either to soma or dendrites. The effect consisted in shortening spike response latencies and increasing response intensity and duration. The modified responses were recorded within 1-min interval after acetylcholine microinjections at a distance within 300 microns of the soma. Parameters of responses to somatic applications of excitatory amino acids were not significantly changed by acetylcholine. The results suggest that acetylcholine improves dendritic propagation rather than membrane excitability.

Discharge patterns were studied in response to iontophoretic application of acetylcholine to the soma and dendrites of 128 neocortical pyramidal neurons of layer V. Extracellular recordings were obtained from slices of the guinea-pig parietal cortex. All responses found were excitatory and were better expressed in spontaneously firing cells than in silent ones. Sensitivity to acetylcholine was approximately the same at somatic and dendritic sites in all the cells. Activation of muscarinic receptors gave rise to firing patterns with equal latencies and intensities when applied to both soma and dendrites. The latter suggests that membrane excitation elicited in dendrites by binding of acetylcholine to muscarinic cholinoreceptors is likely to propagate towards the soma through intracellular biochemical processes. Modulating effect of acetylcholine on output firing patterns, elicited by dendritic application of excitatory amino acids, included shortening of the somatic response latency and increase of response intensity and duration. We propose that, in contrast to glutamatergic excitation, the spread of cholinergic excitation along dendrites involves intra-cellular chemical signalling and results in changing the electrical properties of dendrites all over their length.

Spike responses of neurons to the microiontophoretic application of acetylcholine to the soma and the dendrites were studied. The somatic and dendritic membranes had virtually equal sensitivity to acetylcholine. Only activatory responses were seen, which were most typical of spontaneously active neurons. Muscarinic activation induced spike responses with equal latent periods and equal intensities on application of acetylcholine to dendrites and the soma. It is suggested that intracellular chemical signaling is involved in the propagation of cholinergic excitation via dendrites.

In slices of parietal cortex of a guinea pig spike reactions were studied induced in neurons by iontophoretical applications of acetylcholine to their somata and dendrites. The results were obtained from 128 units. When applied to different sites of the neuronal membrane acetylcholine produced an increase in firing activity nearly in the same percent of cases (50-75%). The reactions to acetylcholine were most typical for spontaneously active neurons. The slow onset (to 8 sec) and long duration (to 25 sec) of responses evoked by acetylcholine point to an involvement of muscarinic receptors. Spike responses evoked by acetylcholine application to soma and dendrites were of the same latencies and magnitude. The most distant dendritic site where the acetylcholine excitation was able to evoke response of the soma was separated from it by 300-400 mcm. It is suggested that acetylcholine excitation propagates from dendritic points to the soma with intracellular biochemical processes.

On guinea-pig neocortical slices the spatial organization of dendrites sensitive to excitatory amino acids was studied. Extracellular recording were obtained from the the soma of layer V neurons. Responses of 135 neurons to iontophoretically applied glutamate or aspartate have been analysed. An increased firing rate to somatic and most of dendritic applications were of short latency not exceeding 500 ms. Dendritic applications caused somatic responses with far longer latencies (up to 2-3 s) in 18% of cases. Latencies of responses to excitatory amino acids applied to several dendritic sites of the same neuron had similar values. The greatest reactions were obtained in response to excitatory amino acids imposed to the soma and proximal dendrites. At a distance of 100 microm beyond the soma in the basal region and region and further than 300 microm in the apical region excitatory amino acid applications produced two to three times less intensive somatic response. The area where dendritic activation gave rise to change in neuronal firing was confined to 350 and 800 microm for basal and apical dendrites, respectively. Topography of effective dendritic sites fell into the area corresponding to anatomically known outline of dendritic tree of pyramidal neurons. This fact implies that in our experiments we basically dealt with layer V pyramids. The results obtained suggest that local activation of distal dendrites may elicit spike generation in the soma. Different electrical properties of somatic and dendritic membranes are discussed.

1. The properties of a well-defined type of GABAergic local circuit neuron, the axo-axonic cell (n = 17), were investigated in rat hippocampal slice preparations. During intracellular recording we injected axo-axonic cells with biocytin and subsequently identified them with correlated light and electron microscopy. Employing an immunogold-silver intensification technique we showed that one of the physiologically characterized cells was immunoreactive for gamma-aminobutyric acid (GABA). 2. Axo-axonic cells were encountered in the dentate gyrus (n = 5) as well as subfields CA3 (n = 2) and CA1 (n = 10). They generally had smooth, beaded dendrites that extended throughout all hippocampal layers. Their axons ramified densely in the cell body layers and in the subjacent stratum oriens or hilus, respectively. Tested with electron microscopy, labeled terminals (n = 53) established synapses exclusively with the axon initial segment of principal cells in strata oriens and pyramidale and rarely in lower radiatum. Within a 400-microns slice a single CA1 axo-axonic cell was estimated to be in synaptic contact with 686 pyramidal cells. 3. Axo-axonic cells (n = 14) had a mean resting membrane potential of -65.1 mV, an average input resistance of 73.9 M omega, and a mean time constant of 7.7 ms. Action potentials were of short duration (389-microseconds width at half-amplitude) and had a mean amplitude of 64.1 mV. 4. Nine of 10 tested cells showed a varying degree of spike frequency adaptation in response to depolarizing current injection. Current-evoked action potentials were usually curtailed by a deep (10.2 mV) short-latency afterhyperpolarization (AHP) with a mean duration of 28.1 ms. 5. Cells with strong spike frequency accommodation (n = 5) had a characteristic firing pattern with numerous spike doublets. These appeared to be triggered by an underlying depolarizing afterpotential. In the same cells, prolonged bursts of action potentials were followed by a prominent long-duration AHP with a mean time constant of 1.15 s. 6. Axo-axonic cells responded to the stimulation of afferent pathways with short-latency excitatory postsynaptic potentials (EPSPs) or at higher stimulation intensity with up to three action potentials. Axo-axonic cells in the dentate gyrus could be activated by stimulating the CA3 area as well as the perforant path, whereas in the CA1 area responses were elicited after shocks to the perforant path, Schaffer collaterals, and the stratum oriens-alveus border. 7. In the CA1 area the EPSP amplitude increased in response to membrane hyperpolarization.(ABSTRACT TRUNCATED AT 400 WORDS)

The background firing activity was recorded extracellularly in experiments on guinea-pig neocortical slices maintained in vitro. The following types of background firing activity were revealed: (i) high regular single spikes (48%), (ii) irregular single spikes (15%), (iii) bursts (7%), (iv) groups (7%), (v) mixed activity where single spikes alternated with bursts or groups (28%). The specific interspike interval distribution and the specific shape of autocorrelogram corresponded to each of these background firing activity types. Furie analysis of autocorrelograms showed periodic components in spike sequences with the maxima at 3, 12, and 28 Hz. When blocking synaptic transmission with 100 mM adenosine, about 70% of the background active cells "fell silent" and the remaining 30% of neurons continued to generate action potentials. The latter seem to be actual spontaneously active neurons, i.e. they were capable of autonomous spike generation. We failed to find any correlation between the type of neuronal firing and the ability of neurons to be spontaneously active. The selective blockade of inhibitory synapses with 100 mM picrotoxine did not practically change the character of background firing activity though the responses to stimulation became epileptic. An important conclusion to emerge from this study is that the background firing activity in cortical slices can include the actual spontaneous discharges related to intrinsic cell properties as well as those concerned with synaptic actions. Furthermore, a small number of spontaneously active neurons seem to be able to synaptically activate twice the number of cells. The inhibitory interneurons did not significantly influence the propagation of excitation with the absence of stimulation.

The dependence of evoked neuronal discharges on stimulus electrode position, on power, frequency and duration of stimulation was investigated in guinea-pig neocortical slices. At suprathreshold stimulus intensity and under low frequency (about 0.1/s) and limited duration of stimulus series (10-30) the discharge pattern was usually well preserved. A, more intensive, higher-frequency or too long stimulation often led to transient habituation of responses. When the distances from neuron to the stimulus sites were equal the radial propagation of excitation seemed to be easier than tangential one.

The investigation of the ratio of neuronal cytoplasmic RNA mono- and two-chain sections was performed in thin neocortical slices by means of fluorescent probing. Changes of the relative intensity of Acridine Orange red luminescence indicated a decrease in the quota of monochain sections in the common neuronal RNA pool. In eight hours of superfusion it was authentically less than in the control slices and in the slices which were incubated during four hours. Deep profiles of red fluorescence became plane in the process of incubation. It was suggested that the essential decrease in the red luminescence intensity of cytoplasmic RNA in vitro, observed between four and eight hours, may be conditioned by disintegration of polysomes to monosomes, and this, in its turn, may cause the decrease in ribosomal synthetic activity.

The propagation of excitation has been studied in neocortical guinea pig slices maintained in vitro. Stimuli were given at different distances from the chosen neuron. Comparison of latencies of the evoked impulse reactions indicated a complex structure and configuration of the vertically oriented ensemble of comparatively synchronously activated neurons. It is assumed that such an ensemble has a narrow (less than 300 microns) top layer II, a wide (approximately 600 microns and more) middle part in layer V and a narrowed (approximately 300 microns) basis in layer VI. A heterogeneity of the ensemble (the module) with respect to vertical propagation of excitation is found.

Background neuronal discharges were recorded extracellularly in guinea pig cerebral cortex slices maintained in vitro. Synaptic disconnection was reached by adding 100 microM of adenosine or 20-75 microM of 5-AMP. It was shown that 30-40% of neurons continued to discharge even under conditions of complete synaptic blockade, although the discharge frequency decreased. These neurons were considered as endogenously active ones. Other cells stopped their activity as soon as adenosine reached the slice, but they began to generate action potentials again after superfusion with normal solution. These cells were synaptically activated and were considered as exogenously active. In particular most cells with initially very regular discharges became silent during blockade and restored their activity after superfusion with normal saline. This fact suggests the existence of reverberating activity in neocortical slices with periods of 30-140 ms.

In defensive conditioning of rabbits to light the pattern of the background spike activity (BSA) in the visual cortex did not change in average as compared to the control. In the sensorimotor cortex the spike activity pattern was changed: the afterdischarge inhibition increased. Cells with a regular component in BSA divided into two groups according to the duration of bursts and of inhibitory pauses in the BSA. In the course of conditionings, the number of neurones with non-random components in BSA in the sensorimotor area did not change, while it decreased in the visual area due to a reduction in number of elements with short groups of discharges.

The character of statistic connection between the background spike activity of neurones in the visual and sensorimotor cortical areas was studied in calm awake rabbits and in the course of defensive conditioning to light. The obtained cross-correlation functions showed that in the process of establishment of temporary connection, there was a decline in the correlation level between discharges of neighbouring neurones in both areas and a rise in correlation between discharges of remote neurones, one of which was located in the visual area and the other--in the sensorimotor area.

Spontaneous firing of single neurons was studied during L-glutamate-induced stable activation in the cat cerebral cortex. The autocorrelation analysis of the trains of discharges showed, that periods of increased and decreased probability of discharges were constant in general. A single neuron seems incapable of radically affecting the interneurons connected with it. The type of spontaneous firing is mainly associated with the level of activation of the neuronal assembly where the neuron belongs.

Investigation of the picrotoxin effect on the background neuronal activity was performed in guinea-pig cerebral cortex slices maintained in vitro. Extracellular recording showed that the blockade of GABA-ergic inhibition did not change characteristics of spike trains significantly, although the stimulus-evoked reactions became epileptic. These data give evidence that the inhibition processes in the background activity of cortex slices are expressed extremely slightly and have no intrinsic influence on this activity.

The types and values of statistic dependence between extracellularly recorded background spike activity of cortical neurones and EEG oscillations were studied in chronic experiments on intact alert rabbits. It was shown that the relative number of neurones with discharges significantly connected with EEG slow components, remains practically the same in different functional states of the brain and amounts to about 80%. The mean level of the studied dependence changes following transitions from one state to another:it is lowered during the extinction of orienting reaction and continues to go down during the elaboration of the conditioned reflex.

The background impulse activity of individual neurons was recorded extracellularly in the cerebral cortex of the cat during the prolonged microionophoretic delivery to these neurons of L-glutamate. Glutamate ionophoresis ensured the transition of the neuron to an elevated, but stable level of activation. An autocorrelation analysis of the trains of impulses showed that in spite of the multiple rise in the average discharge frequency, the type of background impulse activity and the periods of increased and reduced probability of discharges for the most part remained constant. The obtained data indicate that an individual cell is unable to influence substantially the interneurons connected with it, while the type of background activity of a neuron is determined primarily by the level of activation of the cellular ensemble incorporating this neuron.

Background neuronal discharges were recorded extracellularly in guinea-pig cerebral cortex slices maintained in vitro. Six types of activity were classified: 1--regular single discharges (38.5%), 2--irregular spikes (6.5%), 3--bursts (6.5%), 4--burst-single discharge (mixed) activity (26.5%), 5--group discharges (2.5%), 6--multineuronal volleys (18.5%). Characteristic distributions of interspike intervals and autocorrelograms corresponded to each of these types of activity. Regular pacemaker-type discharges were most peculiar under conditions of small volumes of the grey matter and lack of afferentation. Such regular activity was never observed in normal or isolated neocortex.

Spike activity of cortical units and EEG on the surface of the cortex near the microelectrode were recorded simultaneously in alert non-immobilized rabbits. The technique of synchronous summation used in the data processing has revealed a statistically significant interrelation between background spike activity of the cortical units and the surface biopotentials. A predominant tendency was revealed toward generation of a spike during the maximal surface-positive deviation of slow biopotentials. The average level of cross-correlation between the spike and slow processes in the background has a magnitude of the order of +/- 10(-2), while individual cross correlation coefficients attain the magnitude of 10-1.

The correlation between the spike activity and the waves of surface ECoG was studied in the visual and motor cortex of alert non-immobilized rabbits. A comparison was made between the forms of the obtained correlation functions for the cells with different horizontal and vertical distances between them. The analysis revealed the similarity of such functions in neurones of V-VI cortical layers, situated vertically under each other along the microelectrode track. Similar in form statistic connections between the ECoG and the background spike activity were also found, beginning with the depth of 1.000 mc, in the neurones situated at the same horizontal cortical level; however, with the increased distance between the cells the degree of similarity of these connections decreases in a non-linear way. On the basis of the obtained data a conclusion is made that the tangential sizes of the background functional ensembles of the cortical neurones are within the range of 140-300 mc.

Elaboration of electro-defensive conditioned reflex to light in rabbits has shown, that the values of summary cross-correlation coefficients between EEG and impulse activity of cortical neurones do not change significantly as compared to the control. In these conditions does not change either the part of the neurones that have a significant correlation of their impulse discharges with any configuration of the EEG wave. During 6--10 days of conditioning there occurs a decrease in the amplitudes of both positive and negative EEG oscillations, connected with unit discharges in the neocortex. An EEG quantum has being recorded, i. e. a depolarization potential, directly connected with the generation of spikes by the studied nervous elements.