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46 results on '"Karpova, Olga V."'

9. Novel Universal Recombinant Rotavirus A Vaccine Candidate: Evaluation of Immunological Properties

Author

Granovskiy, Dmitriy L., primary, Khudainazarova, Nelli S., additional, Evtushenko, Ekaterina A., additional, Ryabchevskaya, Ekaterina M., additional, Kondakova, Olga A., additional, Arkhipenko, Marina V., additional, Kovrizhko, Marina V., additional, Kolpakova, Elena P., additional, Tverdokhlebova, Tatyana I., additional, Nikitin, Nikolai A., additional, and Karpova, Olga V., additional

Published
2024
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11. Semiempirical charge distribution of clusters in the ion sputtering of metal

Author

Matveev, Victor I. and Karpova, Olga V.

Subjects
Physics - Atomic and Molecular Clusters, Physics - Instrumentation and Detectors
Abstract

We propose the generalization of a known established empirically (Wahl W. and Wucher A. Nucl. Instrum. Meth. B 94, 36(1994)) power law, describing relative mass-spectra of neutral sputtered clusters, on the cases of arbitrary cluster charges. The fluctuation mechanism of charge state formation of sputtering products in the form of large clusters with the number of atoms N > 5 is also proposed. The simple formula obtained by us has been shown a good agreement with the experimental data., Comment: (minor changes)LaTeX, 8 pages, 3 Postscript figures submitted to: Physical Review A

Published
1999

17. Increased Efficiency of Radiation Inactivation of Virions by Gold Nanoparticles

Author

Nikitin, Nikolai A., primary, Arkhipenko, Marina V., additional, Dement'eva, Olga V., additional, Kartseva, Maria E., additional, Shishmakova, Elena M., additional, Sanochkina, Elizaveta V., additional, Shiryaeva, Ekaterina S., additional, Kolyvanova, Maria A., additional, Belousov, Alexandr V., additional, Rudoy, Victor M., additional, Feldman, Vladimir I., additional, Karpova, Olga V., additional, and Morozov, Vladimir N., additional

Published
2022
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18. New formulation of a recombinant anthrax vaccine stabilised with structurally modified plant viruses

Author

Granovskiy, Dmitriy L., primary, Ryabchevskaya, Ekaterina M., additional, Evtushenko, Ekaterina A., additional, Kondakova, Olga A., additional, Arkhipenko, Marina V., additional, Kravchenko, Tatiana B., additional, Bakhteeva, Irina V., additional, Timofeev, Vitalii S., additional, Nikitin, Nikolai A., additional, and Karpova, Olga V., additional

Published
2022
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26. Various Adjuvants Effect on Immunogenicity of Puumala Virus Vaccine

Author

Kurashova, Svetlana S., primary, Ishmukhametov, Aidar A., additional, Dzagurova, Tamara K., additional, Egorova, Maria S., additional, Balovneva, Maria V., additional, Nikitin, Nikolai A., additional, Evtushenko, Ekaterina A., additional, Karpova, Olga V., additional, Markina, Anna A., additional, Aparin, Peter G., additional, Tkachenko, Petr E., additional, L`vov, Vyatcheslav L., additional, and Tkachenko, Evgeniy A., additional

Published
2020
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30. Surface characterization of the thermal remodeling helical plant virus

Author

Ksenofontov, Alexander L., primary, Fedorova, Natalia V., additional, Badun, Gennady A., additional, Serebryakova, Marina V., additional, Nikitin, Nikolai A., additional, Evtushenko, Ekaterina A., additional, Chernysheva, Maria G., additional, Bogacheva, Elena N., additional, Dobrov, Eugeny N., additional, Baratova, Ludmila A., additional, Atabekov, Joseph G., additional, and Karpova, Olga V., additional

Published
2019
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32. Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus

Author

Donchenko, Ekaterina K., Pechnikova, Evgeniya V., Mishyna, Maryia Yu, Manukhova, Tatiana I., Sokolova, Olga S., Nikitin, Nikolai A., Atabekov, Joseph G., Karpova, Olga V., Donchenko, Ekaterina K., Pechnikova, Evgeniya V., Mishyna, Maryia Yu, Manukhova, Tatiana I., Sokolova, Olga S., Nikitin, Nikolai A., Atabekov, Joseph G., and Karpova, Olga V.

Abstract

Plant viruses and their virus-like particles (VLPs) have a lot of advantages for biotechnological applications including complete safety for humans. Alternanthera mosaic virus (AltMV) is a potentially promising object for design of novel materials. The 3D structures of AltMV virions and its VLPs were obtained by single particle EM at ~13Å resolution. The comparison of the reconstructions and a trypsin treatment revealed that AltMV CPs possesses a different fold in the presence (virions) and absence of viral RNA (VLPs). For the first time, the structure of morphologically similar virions and virus-like particles based on the coat protein of a helical filamentous plant virus is shown to be different. Despite this, both AltMV virions and VLPs are stable in a wide range of conditions. To provide a large amount of AltMV for biotechnology usage the isolation procedure was modified.

Published
2017

33. Two approaches for the stabilization of Bacillus anthracisrecombinant protective antigen

Author

Ryabchevskaya, Ekaterina M., Evtushenko, Ekaterina A., Granovskiy, Dmitry L., Ivanov, Peter A., Atabekov, Joseph G., Kondakova, Olga A., Nikitin, Nikolai A., and Karpova, Olga V.

Abstract

ABSTRACTAnthrax is a zoonotic disease caused by the gram-positive spore-forming bacteria Bacillus anthracis. There is a need for safe, highly effective, long-term storage vaccine formulations for mass vaccination. However, the development of new subunit vaccines based on recombinant protective antigen (rPA) faces the problem of vaccine antigen instability. Here, the potential of simultaneous application of two different approaches to stabilize rPA was demonstrated. Firstly, we employed spherical particles (SPs) obtained from the tobacco mosaic virus (TMV). Previously, we had reported that SPs can serve as an adjuvant and platform for antigen presentation. In the current work, SPs were shown to increase the stability of the full-size rPA without loss of its antigenic properties. The second direction was site-specific mutagenesis of asparagine residues to avoid deamidation that causes partial protein degradation. The modified recombinant protein comprising the PA immunogenic domains 3 and 4 (rPA3 + 4) was stable during storage at 4 and 25°C. rPA3 + 4 interacts with antibodies to rPA83 both individually and as a part of a complex with SPs. The results obtained can underpin the development of a recombinant vaccine with a full-size modified rPA (with similar amino acid substitutions that stabilize the protein) and SPs.

Published
2021
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35. Study of rubella candidate vaccine based on a structurally modified plant virus

Author

Trifonova, Ekaterina A., primary, Zenin, Vladimir A., additional, Nikitin, Nikolai A., additional, Yurkova, Maria S., additional, Ryabchevskaya, Ekaterina M., additional, Putlyaev, Egor V., additional, Donchenko, Ekaterina K., additional, Kondakova, Olga A., additional, Fedorov, Alexey N., additional, Atabekov, Joseph G., additional, and Karpova, Olga V., additional

Published
2017
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36. Proteins immobilization on the surface of modified plant viral particles coated with hydrophobic polycations

Author

Nikitin, Nikolai A., Malinin, Andrei S., Trifonova, Ekaterina A., Rakhnyanskaya, Anna A., Yaroslavov, Aleksandr A., Karpova, Olga V., and Atabekov, Joseph G.

Subjects
fungi
Abstract

Two hydrophobic cations based on poly-N-ethyl-vinylpyridine were used to produce biologically active complexes. The complexes obtained from tobacco mosaic virus (TMV) spherical particles (SPs), hydrophobic polycation, and a model protein were stable and did not aggregate in solution, particularly at high ionic strengths. The nucleic acid-free SPs were generated by thermal remodeling of the TMV (helical rod-shaped plant virus). The model protein preserved its antigenic activity in the ternary complex (SP–polycation–protein). Immobilization of proteins on the surface of SPs coated with hydrophobic cation is a promising approach to designing biologically active complexes used in bionanotechnologies.

Published
2014
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44. β-structure of the coat protein subunits in spherical particles generated by tobacco mosaic virus thermal denaturation

Author

Dobrov, Evgeny N., Nikitin, Nikolai A., Trifonova, Ekaterina A., Parshina, Evgenia Yu., Makarov, Valentin V., Maksimov, George V., Karpova, Olga V., and Atabekov, Joseph G.

Abstract

Conversion of the rod-like tobacco mosaic virus (TMV) virions into “ball-like particles” by thermal denaturation at 90–98 °C had been described by R.G. Hart in 1956. We have reported recently that spherical particles (SPs) generated by thermal denaturation of TMV at 94–98 °C were highly stable, RNA-free, and water-insoluble. The SPs were uniform in shape but varied widely in size (53–800 nm), which depended on the virus concentration. Here, we describe some structural characteristics of SPs using circular dichroism, fluorescence spectroscopy, and Raman spectroscopy. It was found that the structure of SPs protein differs strongly from that of the native TMV and is characterized by coat protein subunits transition from mainly (about 50%) α-helical structure to a structure with low content of α-helices and a significant fraction of β-sheets. The SPs demonstrate strong reaction with thioflavin T suggesting the formation of amyloid-like structures.

Published
2014
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45. Translation arrest of potato virus X RNA in Krebs-2 cell-free system: RNase H cleavage promoted by complementary oligodeoxynucleotides

Author

Miroshnichenko, Nadezhda A., Karpova, Olga V., Morozov, Sergei Yu., Rodionova, Nina P., and Atabekov, Joseph G.

Abstract

Translation arrest of genomic potato virus X (PVX) RNA promoted by complementary oligodeoxynucleotides in Krebs-2 cell-free system is described. 14–15 mer oligodeoxynucleotides complementary to the 5′-proximal cistron of PVX RNA were shown to induce specific truncation of the major non-structural polypeptide coded by PVX RNA. Evidence is presented that effective translational arrest of PVX RNA in the presence of complementary oligonucleotides restults from the site-specific cleavage of RNA by endogenous RNase H intrinsic to the Krebs-2 extract. No similar translational arrest was found in the rabbit reticulocyte lysate cell-free system.

Published
1988
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46. Characterization of Alternanthera mosaic virus and its Coat Protein.

Author

Mukhamedzhanova AA, Smirnov AA, Arkhipenko MV, Ivanov PA, Chirkov SN, Rodionova NP, Karpova OV, and Atabekov JG

Abstract

A new isolate of Alternantheramosaic virus (AltMV-MU) was purified from Portulaca grandiflora plants. It has been shown that the AltMV-MU coat protein (CP) can be efficiently reassembled in vitro under different conditions into helical RNA-free virus-like particles (VLPs) antigenically related to native virus. The AltMV-MU and VLPs were examined by atomic force and transmission electron microscopies. The encapsidated AltMV-MU RNA is nontranslatable in vitro. However, it can be translationally activated by CP phosphorylation or by binding to the TGB1protein from the virus-coded movement triple gene block.

Published
2011
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