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Your search keyword '"Karthik, Yalpi"' showing total 14 results
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14 results on '"Karthik, Yalpi"'

2. Immunoinformatic exploration of a multi-epitope-based peptide vaccine candidate targeting emerging variants of SARS-CoV-2

Author

Kumar, K. M., primary, Karthik, Yalpi, additional, Ramakrishna, D., additional, Balaji, S., additional, Skariyachan, Sinosh, additional, Murthy, T. P. Krishna, additional, Sakthivel, Kunnathur Murugesan, additional, Alotaibi, Badriyah S., additional, Shukry, Mustafa, additional, Sayed, Samy M., additional, and Mushtaq, Muntazir, additional

Published
2023
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4. In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Author

Kenchappa, Pratap G., primary, Karthik, Yalpi, additional, Vijendra, Poornima D., additional, Hallur, Raghavendra L. S., additional, Khandagale, Ajay S., additional, Pandurangan, Ashok K., additional, Jayanna, Sathisha G., additional, Alshehri, Mohammed Ali, additional, Alasmari, Abdulrahman, additional, Sayed, Samy, additional, Shantaram, Manjula, additional, and Mushtaq, Muntazir, additional

Published
2023
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6. Antiproliferative activity of antimicrobial peptides and bioactive compounds from the mangrove Glutamicibacter mysorens

Author

Karthik, Yalpi, primary, Ishwara Kalyani, Manjula, additional, Krishnappa, Srinivasa, additional, Devappa, Ramakrishna, additional, Anjali Goud, Chengeshpur, additional, Ramakrishna, Krishnaveni, additional, Wani, Muneeb Ahmad, additional, Alkafafy, Mohamed, additional, Hussen Abduljabbar, Maram, additional, Alswat, Amal S., additional, Sayed, Samy M., additional, and Mushtaq, Muntazir, additional

Published
2023
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9. Occurrence of Streptomyces tauricus in mangrove soil of Mangalore region in Dakshina Kannada as a source for antimicrobial peptide.

Author

Karthik, Yalpi and Kalyani, Manjula Ishwara

Subjects
ANTIMICROBIAL peptides, STREPTOMYCES, MANGROVE swamps, PRECIPITATION (Chemistry), MOLECULAR weights, BACILLUS cereus, MOUNTAIN soils
Abstract

Microbial resistance and deprivation of the effective drugs have become the foremost problem that propels to seek out for advanced approach. This concept initiated a need to search for more effective antimicrobial compounds from reliable sources. The Streptomyces is grouped under phylum Actinobacteria and are considered prolific producers of antibiotics, around 70% of presently available antibiotics are contributed by Streptomyces alone. In this study, Mangroves of the Mangalore Coast offered a unique source for screening Actinomyces group of microorganisms. We investigated on the four soil samples collected from Mangrove swamps of Mangalore, Karnataka, India. Based on their culture traits, the 18 distinct Actinomyces isolates were analyzed through a series of morphological and biochemical tests on starch casein nitrate (SCN) media. Culture biomasses were subjected for intracellular protein extraction through acetone precipitation method; the extracted proteins from each Actinomyces isolate were examined for antimicrobial activity against test organisms. The isolate ANTB‐YKMU4 showed potential antimicrobial activity against significant number of test organisms; Bacillus cereus, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, and Pseudomonas aeruginosa. The isolate ANTB‐YKMU4 through 16 s rRNA gene sequence analysis was identified as Streptomyces tauricus strain with GenBank accession no. MW785875.1. The S. tauricus further cultivated for efficient biomass growth on SCN media for subsequent protein extraction and purification by a series of Electrophoretic and chromatographic techniques. Thus, by intracellular extractions from S. tauricus resulted in the identification of peptide with a molecular weight of 266 Da that was characterized by LC‐MS. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Extraction, purification, characterization and evaluation of antibacterial and anticancer activity of β,ε-Carotene-3,3′-diol (Lutein) from Bacillus endophyticus- AVP9

Author

Sairam Mantri, Chandini Sultana Syed, Samuel John Konatham, Ponraj P, Karthik Yalpi, Amrutha Valli Audipudi, and Krishnaveni Ramakrishna

Abstract

A non-fluorescent pigment produced from the fluorescent Bacillus endophyticus AVP-9 which possess abundant lutein content considered as a xanthophyl-type compound with potentially beneficial pharmacological properties for enhancing human health. In this context, bacterial lutein was extracted by employing different solvents to isolate from Bacillus endophyticus was examined in this study. Chromatographically purified lutein was spectrally characterized using ultraviolet-visible spectrometry and spectral analysis was carried through liquid chromatography-mass spectrometry, functional group identification was done by using fourier transform-infrared spectroscopy, structural determination by nuclear magnetic resonance spectrometry. By using hexane and ethyl acetate (7:3) with the flow rate of 5ml/min, was proven to be an excellent solvent phase for separation of lutein, meaning that up to 90% pure lutein generated with a 60% estimated yield. The purified compound identified as a β, ε-3, 31-diol (lutein) having molecular weight of 568.6g/mol with the molecular formula C40H56O2 which exhibits potential antibacterial activity against E. coli (35mm) followed by S. typhi (33mm), S. flexneri (30mm), P. aureginosa (29mm), M. lutea (28mm), P. vulgaris (24mm), S. aureus (21mm) and lowest antibacterial activity against V. cholera (19mm). Purified pigment shows concentration dependent antioxidant activities with IC50 values of pigment was found to be 4.5 mg/ml. Whereas anti-proliferation activity pigment shows considerable effect MCF-7 and LNCaP cell lines, when treated with 100µg/ml, cell viability was decreased to 36.363% at IC50 value of 21.935 µg/ml and for LNCaP, the cell viability was decreased to 42.449% at IC50 value of 56.937 µg/ml.

Published
2022

14. Exploration of bioactive compounds from Olea dioicain Western Ghats of Karnataka using GC–MS

Author

Krishnappa, Srinivasa, Karthik, Yalpi, Pratap, G. K., Shantaram, Manjula, Umarajashekhar, Alavala, Soumya, J., Bhatt, Bhagyashree, Sayed, Samy M., Alhelaify, Seham Sater, Aharthy, Ohud Muslat, and Mushtaq, Muntazir

Abstract

Bioactive compounds in plants are essential for the formation of novel chemotherapeutic drugs, which have been used in Ayurveda to treat a variety of illnesses. Indian medicinal herbs have been used for thousands of years to treat a variety of illnesses, such as fever, cancer, snake bites, rheumatism, skin problems, and neurodegenerative diseases. GC–MS was used to locate and categorize bioactive components in Olea dioicaleaves. The results showed that presence of octanoic acid, methyl ester, decanoic acid, methyl ester, desulphosinigrin, l-gala-l-ido-octose, methyl tetradecanoate, Tetradecanoic acid, 6-benzoxazolesulfonamide, N-(2-hydroxyethyl)-2-methyl-, 10-chloro-5-methoxy-5H-dibenzo[a,d][7]annulene, pentadecanoic acid, oleic acid, n-hexadecanoic acid, hexanedioic acid, dioctyl ester, and squalene. The methanol extract of Olea dioicawas effective against a wide spectrum of pathogenic bacteria at four different concentrations, with the highest activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Xanthomonas campestris,and Salmonella typhimurium.It also showed moderate activity against Agrobacterium tumefaciens, Pseudomonas aeruginosa, Streptomyces pneumonia,and Pseudomonas syringae.The pharmacological properties of O. dioica, as well as their variety and comprehensive phytochemistry, could be exploited as a potent antimicrobial agent for future therapeutics.

Published
2024
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