Search

Your search keyword '"Karvinen, Henna"' showing total 16 results
Start Over Author "Karvinen, Henna"
16 results on '"Karvinen, Henna"'

7. Growth factor expression in atherosclerosis and gene transfer for therapeutic angiogenesis

Author

Karvinen, Henna, A.I. Virtanen -instituutti, A.I. Virtanen Institute for Molecular Sciences, Terveystieteiden tiedekunta, A.I. Virtanen -instituutti, Bioteknologia ja molekulaarinen lääketiede, Faculty of Health Sciences, A.I. Virtanen Institute for Molecular Sciences, Biotechnology and Molecular Medicine, Terveystieteiden tiedekunta, and Faculty of Health Sciences

Published
2011

8. VEGF-A and PDGF-B combination gene therapy prolongs angiogenic effects via recruitment of interstitial mononuclear cells and paracrine effects rather than improved pericyte coverage of angiogenic vessels

Author

Korpisalo, Petra, Karvinen, Henna, Rissanen, Tuomas T., Kilpijoki, Johanna, Marjomäki, Varpu, Baluk, Peter, McDonald, Donald M., Cao, Yihai, Eriksson, Ulf, Alitalo, Kari, and Ylä-Herttuala, Seppo

Subjects
Vascular Endothelial Growth Factor A, Time Factors, Myocytes, Smooth Muscle, Neovascularization, Physiologic, Genetic Therapy, Proto-Oncogene Proteins c-sis, Article, Adenoviridae, Capillary Permeability, Platelet Endothelial Cell Adhesion Molecule-1, Cell Movement, Paracrine Communication, Animals, Humans, Rabbits, Muscle, Skeletal, Pericytes, Cell Proliferation, Ultrasonography
Abstract

Vessel stabilization and the inhibition of side effects such as tissue edema are essential in angiogenic gene therapy. Thus, combination gene transfers stimulating both endothelial cell and pericyte proliferation have become of interest. However, there is currently little data to support combination gene transfer in large animal models. In this study, we evaluated the potential advantages of such a strategy by combining the transfer of adenoviral (Ad) vascular endothelial growth factor (VEGF)-A and platelet-derived growth factor (PDGF)-B into rabbit hindlimb skeletal muscle. AdLacZ alone or in combination with AdVEGF-A were used as controls. Contrast-enhanced ultrasound, modified Miles assay, and immunohistology were used to quantify perfusion, vascular permeability, and capillary size, respectively. Confocal microscopy was used in the assessment of pericyte-coverage. The transfer of AdPDGF-B alone and in combination with AdVEGF-A induced prominent proliferation of alpha-smooth muscle actin-, CD31-, RAM11-, HAM56-, and VEGF- positive cells. Although, pericyte recruitment to angiogenic vessels was not improved, combination gene transfer induced a longer-lasting increase in perfusion in both intact and ischemic muscles than AdVEGF-A gene transfer alone. In conclusion, intramuscular delivery of AdVEGF-A and AdPDGF-B, combined, resulted in a prolonged angiogenic response. However, the effects were most likely mediated via paracrine mechanisms rather than an increase in vascular pericyte coverage.

Published
2008

10. Capillary enlargement, not sprouting angiogenesis, determines beneficial therapeutic effects and side effects of angiogenic gene therapy

Author

Korpisalo, Petra, primary, Hytönen, Jarkko P., additional, Laitinen, Johannes T.T., additional, Laidinen, Svetlana, additional, Parviainen, Henna, additional, Karvinen, Henna, additional, Siponen, Jaana, additional, Marjomäki, Varpu, additional, Vajanto, Ismo, additional, Rissanen, Tuomas T., additional, and Ylä-Herttuala, Seppo, additional

Published
2010
Full Text
View/download PDF

11. The effects of VEGF-R1 and VEGF-R2 ligands on angiogenic responses and left ventricular function in mice

Author

Huusko, Jenni, primary, Merentie, Mari, additional, Dijkstra, Marike H., additional, Ryhänen, Minttu-Maria, additional, Karvinen, Henna, additional, Rissanen, Tuomas T., additional, Vanwildemeersch, Maarten, additional, Hedman, Marja, additional, Lipponen, Jukka, additional, Heinonen, Suvi E., additional, Eriksson, Ulf, additional, Shibuya, Masabumi, additional, and Ylä-Herttuala, Seppo, additional

Published
2009
Full Text
View/download PDF

12. Vascular Endothelial Growth Factor-A and Platelet-Derived Growth Factor-B Combination Gene Therapy Prolongs Angiogenic Effects via Recruitment of Interstitial Mononuclear Cells and Paracrine Effects Rather Than Improved Pericyte Coverage of Angiogenic Vessels

Author

Korpisalo, Petra, primary, Karvinen, Henna, additional, Rissanen, Tuomas T., additional, Kilpijoki, Johanna, additional, Marjomäki, Varpu, additional, Baluk, Peter, additional, McDonald, Donald M., additional, Cao, Yihai, additional, Eriksson, Ulf, additional, Alitalo, Kari, additional, and Ylä-Herttuala, Seppo, additional

Published
2008
Full Text
View/download PDF

16. Baculovirus-mediated vascular endothelial growth factor-DΔNΔC gene transfer induces angiogenesis in rabbit skeletal muscle.

Author

Heikura, Tommi, Nieminen, Tiina, Roschier, Miia M., Karvinen, Henna, Kaikkonen, Minna U., Mähönen, Anssi J., Lesch, Hanna P., Rissanen, Tuomas T., Laitinen, Olli H., Airenne, Kari J., and Ylä-Herttuala, Seppo

Abstract

Background Occluded arteries and ischemic tissues cannot always be treated by angioplasty, stenting or by-pass-surgery. Under such circumstances, viral gene therapy may be useful in inducing increased blood supply to ischemic area. There is evidence of improved blood flow in ischemic skeletal muscle and myocardium in both animal and human studies using adenoviral vascular endothelial growth factor (VEGF) gene therapy. However, the expression is transient and repeated gene transfers with the same vector are inefficient due to immune responses. Methods Different baculoviral vectors pseudotyped with or without vesicular stomatitis virus glycoprotein (VSV-G) and/or carrying woodchuck hepatitis virus post-transcriptional regulatory element (Wpre) were tested both in vitro and in vivo. VEGF-DΔNΔC was used as therapeutic transgene and lacZ as a control. In vivo efficacy was evaluated as capillary enlargement and transgene expression in New Zealand White (NZW) rabbit skeletal muscle. Results A statistically significant capillary enlargement was detected 6 days after gene transfer in transduced areas compared to the control gene transfers with baculovirus and adenovirus encoding β-galactosidase ( lacZ). Substantially improved gene transfer efficiency was achieved with a modified baculovirus pseudotyped with VSV-G and carrying Wpre. Dose escalation experiments revealed that either too large volume or too many virus particles caused inflammation and necrosis in the target tissue, whereas 109 plaque forming units injected in multiple aliquots resulted in transgene expression with only mild immune reactions. Conclusions We show the first evidence of biologically significant baculoviral gene transfer in skeletal muscle of NZW rabbits using VEGF-DΔNΔC as a therapeutic transgene. Copyright © 2012 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results