100 results on '"Kashif Jilani"'
Search Results
2. Enhanced visible light-driven photocatalytic degradation of crystal violet dye using Cr doped BaFe12O19 prepared via facile micro-emulsion route
- Author
-
Misbah, Ismat Bibi, Farzana Majid, Shagufta Kamal, Kashif Jilani, Babar Taj, Zarish Nazeer, and Munawar Iqbal
- Subjects
Barium hexaferrite ,Cr doping ,Micro-emulsion ,Visible light ,Photocatalysis ,Crystal Violet dye ,Chemistry ,QD1-999 - Abstract
The Cr ion doping effect on various properties of Cr doped BaCrxFe12-xO19 nanoparticles was investigated, which were synthesized via a facile microemulsion approach and properties were studied using XRD, SEM, FTIR, Raman, photoluminescence and UV–visible techniques along with dielectric, optical and ferroelectric properties. The BaCrxFe12-xO19 structure was hexagonal involving P63/mmc space group with average crystalline size of 9–18 nm. The NPs exhibited agglomerated platelet heterogeneous morphology. The presence of the Ba-O-Fe functional group was also confirmed by FTIR analysis. The PL analysis revealed that the doping reduced the recombination rate and charge (e−-h+) separation is facilitated. The coercivity (Hc) and saturation polarization (Ps) increased with doping content and dielectric loss reduces with frequency and dopant concentration. The dopant contents also increased the AC conductivity and the optical bandgap found in 1.75–2.83 (eV) range. The BaCrxFe12-xO19 exhibited a significantly higher photocatalytic efficiency versus BaFe12O19, and 91 % CV dye was degraded in 90 min under visible light irradiation. Additionally, a recycling experiment was conducted to confirm the stability of the prepared photocatalyst and Cr doped BaCrxFe12-xO19 exhibited excellent stability and reusability. The Cr doping affected the dielectric, optical and ferroelectric properties and based on photocatalytic properties of BaCrxFe12-xO19, it has potential applications for the destruction of dyes in wastewater under visible light exposure, which will make the process highly feasible for photocatalytic applications.
- Published
- 2022
- Full Text
- View/download PDF
3. Antibacterial, Antibiofilm, and Anti-Quorum Sensing Potential of Novel Synthetic Compounds Against Pathogenic Bacteria Isolated From Chronic Sinusitis Patients
- Author
-
Muhammad Asif Rafiq, Muhammad Shahid, Kashif Jilani, and Muhammad Aamir Aslam
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Quorum sensing (QS) is a major controller of virulence and biofilm formation in pathogenic bacteria. The aim of the research was to screen novel synthetic compounds (18) from 2 series (Pyrazole and Diene dione) for quorum sensing and biofilm inhibitory potential against resistant pathogens isolated from patients with chronic sinusitis. Most of the compounds have documented zone of inhibition against Gram positive strains Staphylococcus aureus , Enterococcus faecalis and moderate activity against Gram negative Klebseilla pneumoniae and Proteus mirabilis in comparison with standard antibiotic. Compounds Q1 and Q7 have given the maximum zone of inhibition 18 and 20 mm with MICs 0.312 mg/mL and .156 mg/mL against S aureus and E faecalis, respectively. Some compounds were equally potent at inhibiting the formation of biofilm which later established by phase contrast microscopy. Regarding quorum sensing inhibition, the tested concentration of synthetic compound UA3 0.313 mg/mL inhibited violacein production without decreasing Chromobacterium pseudoviolaceum count which was significantly lower than determined MIC’s. It was depicted from the results that selected compounds exhibited low level of cytotoxicity toward human red blood cells. Hence, these findings revealed that most novel compounds were effective antibacterial, whereas compound UA3 has shared significant anti-quorum sensing potential against Chromobacterium pseudoviolaceum.
- Published
- 2022
- Full Text
- View/download PDF
4. Induction of Erythrocyte Membrane Blebbing by Methotrexate-Induced Oxidative Stress
- Author
-
Tayyba Sattar, Kashif Jilani, Khalida Parveen, Zahid Mushtaq, Haq Nawaz, and Maham Abdul Bari Khan
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Methotrexate (MTX) is a common chemotherapeutical agent and folate antagonist with reported apoptotic activity in nucleated cells. The presented research work was planned to investigate the eryptotic effects of methotrexate after the exposure of erythrocytes to therapeutical doses (10–15 μM) of methotrexate. Eryptosis and the role of calcium in the stimulation of membrane blebbing were evaluated through the determination of mean cell volume. Oxidative stress induced by methotrexate (10–15 μM) was determined by antioxidative enzyme activities. Cytotoxic activity against human erythrocytes was examined through hemolysis assay. Exposure of erythrocytes to methotrexate results in significant reduction of superoxide dismutase, catalase, and superoxide dismutase activities at 10 and 15 μM in comparison to the untreated cells. Erythrocytes mean cell volume (MCV) was increased after 48 hours exposure of erythrocytes to methotrexate (10 μM). Significantly increased hemolysis percentage was observed at 10 μM after 48 hours incubation of erythrocytes with methotrexate. The results of the study suggested that the therapeutical doses (10–15 μM) of methotrexate may lead to increase in eryptotic and hemolytic activity of erythrocytes through free radical generation and subsequent calcium entry.
- Published
- 2022
- Full Text
- View/download PDF
5. Stimulation of Erythrocyte Membrane Blebbing by Bifenthrin Induced Oxidative Stress
- Author
-
Fatima Mukhtar, Kashif Jilani, Ismat Bibi, Zahid Mushtaq, Maham Abdul Bari Khan, and Maria Fatima
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Background Bifenthrin is an insecticide and anti-estrogenic compound primarily used to control residential pests by depolarizing sodium gated voltage channels in the nervous system. Eryptosis, the suicidal death of erythrocytes, featured by PS exposure, membrane blebbing and cell shrinkage. Anemia is an outcome of uncontrolled eryptosis. Research Design In this study, erythrocytes were treated with different concentrations (.5-1-1.5 μM) of bifenthrin over a period of 48 hours. In order to investigate the oxidative stress induced by bifenthrin, catalase, superoxide dismutase, and glutathione peroxidase activities were investigated. Results Obtained data indicated the decrease in the enzymes (superoxide dismutase, glutathione peroxidase, and catalase) activities in bifenthrin treated cells at 1 μM concentration. In addition, measurement of cell size and confirmation of the role of calcium in the stimulation of the eryptotic activity of bifenthrin were performed. A significant increase in mean cell volume was found in the presence of bifenthrin and a decrease in mean cell volume in the presence of calcium channel blocker was observed. Similarly, there was also a significant increase in the percentage of hemolysis indicating the necrotic activity of bifenthrin. Conclusions It is concluded that the indicated doses of bifenthrin triggered oxidative stress which may lead to early cell death by eryptosis and hemolysis.
- Published
- 2022
- Full Text
- View/download PDF
6. Green synthesis of iron oxide nanoparticles using pomegranate seeds extract and photocatalytic activity evaluation for the degradation of textile dye
- Author
-
Ismat Bibi, Nosheen Nazar, Sadia Ata, Misbah Sultan, Abid Ali, Ansar Abbas, Kashif Jilani, Shagufta Kamal, Fazli Malik Sarim, M. Iftikhar Khan, Fatima Jalal, and Munawar Iqbal
- Subjects
Mining engineering. Metallurgy ,TN1-997 - Abstract
Iron oxide nanoparticles (Fe2O3 NPs) were fabricated through green route using pomegranate (Punica granatum) seeds extract. The Fe2O3 NPs were characterized by UV–vis, XRD, EDX, SEM and AFM techniques. The adopted green rout furnished semi spherical Fe2O3 NPs, uniformly distributed and particle size in the range of 25–55 nm. The LCMS/MS was performed for the identification of biomolecule present in the extract of pomegranate seeds and p-hydroxy benzoic acid, gallic acid, methyl gallate, catechin, kaempferol-3-O-sophoroside, 3-deoxyflavonoids, magnolol, ferulic acid, vanillic acid and pinocembrin along with other minor constituents were detected in the extracts using for Fe2O3 NPs. The synthesized Fe2O3 NPs showed excellent photocatalytic activity against reactive blue under UV light irradiation and maximum degradation of 95.08% was achieved with 56 min of reaction time. In view of promising activity, the Fe2O3 NPs could be used photocatalyst for the degradation of dyes in wastewater and pomegranate seeds extract can be applied as eco-benign and cost effective approach for Fe2O3 NPs synthesis. Keywords: Punica granatum, Green synthesis, Iron oxide nanoparticles, Photocatalysis, Dye degradation
- Published
- 2019
- Full Text
- View/download PDF
7. Effect of Fe and Bi doping on LaCoO3 structural, magnetic, electric and catalytic properties
- Author
-
Sara Ajmal, Ismat Bibi, Farzana Majid, Sadia Ata, Kashif Kamran, Kashif Jilani, Shazia Nouren, Shagufta Kamal, Abid Ali, and Munawar Iqbal
- Subjects
Mining engineering. Metallurgy ,TN1-997 - Abstract
La1-xBixCo1-yFeyO3 perovskite was prepared by micro-emulsion method and effect of the Fe and Bi doping on the properties of perovskite was investigated. As-prepared perovskite was characterized by X-ray diffraction (XRD), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Energy-dispersive X-ray (EDX) and atomic force microscopy (AFM) techniques. La1-xBixCo1-yFeyO3 perovskite showed distorted rhombohedral structure and particle size was in the range of 33.05–57.41 nm. Doping of perovskite with Bi and Fe enhanced the direct current (DC) resistivity. Dielectric parameters were studied in the range of 20 to 20 MHz and vibrating magnetometery revealed that the Mr and Ms values were higher for La0.75Bi0.25Co0.75Fe0.25O3, whereas LaCoO3 showed higher value of Hc. Photocatalytic activity (PCA) was evaluated by degrading congo red dye and Bi and Fe doped perovskite showed significantly higher activity versus LaCoO3. Results revealed that the doping of LaCoO3 with Bi and Fe enhanced the magnetic, dielectric and catalytic properties of LaCoO3. Keywords: LaCoO3 perovskite, Bi and Fe doping, Characterization, Properties, Photocatalysis
- Published
- 2019
- Full Text
- View/download PDF
8. Induction of Erythrocyte Shrinkage by Omeprazole
- Author
-
Ayesha Naveed, Kashif Jilani, Abu Bakar Siddique, Muhammad Akbar, Muhammad Riaz, Zahid Mushtaq, Muhammad Sikandar, Sajida Ilyas, Ismat Bibi, Asma Asghar, Ghulam Rasool, and Muhammad Irfan
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Omeprazole, a proton pump inhibitor blocks the H + /K + -ATPase channels of gastric parietal cells. It is used for the treatment of peptic ulcer. Prolonged use of omeprazole may involve in inducing anemia. The key marker of eryptosis includes membrane blebbing, cell shrinkage and phosphatidylserine (PS) exposure at the cell surface. In current study, the eryptotic, oxidative as well as hemolytic effects of therapeutical doses (0.5, 1 and 1.5 µM) of omeprazole were investigated after exposing erythrocytes for 48 hours. Investigation of eryptosis was done by cell size measurement, PS exposure determination and calcium channel inhibition. As a possible mechanism of omeprazole induced eryptosis, oxidative stress was investigated by determining the catalase, glutathione peroxidase and superoxide dismutase activities. Similarly, necrotic effect of omeprazole on erythrocytes was also evaluated through hemolysis measurement. Results of our study illustrated that 1.5 µM of omeprazole may induce significant decrease in superoxide dismutase, glutathione peroxidase and catalase activities as well as triggered the erythrocytes shrinkage, PS exposure and hemolysis. Role of calcium was also confirmed in inducing erythrocyte shrinkage. It is concluded that the exposure of erythrocytes with 1.5 µM omeprazole may enhance the rate of eryptosis and hemolysis by inducing oxidative stress.
- Published
- 2020
- Full Text
- View/download PDF
9. Stimulation of Erythrocyte Membrane Blebbing by Naproxen Sodium
- Author
-
Sajida Ilyas, Kashif Jilani, Muhammad Sikandar, Saba Siddiq, Muhammad Riaz, Ayesha Naveed, Ismat Bibi, Haq Nawaz, Muhammad Irfan, and Asma Asghar
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Naproxen sodium is a nonsteroidal anti-inflammatory drug (NSAID) having antipyretic and analgesic properties, mainly used for the treatment of rheumatoid arthritis and osteoarthritis. Eryptosis is an alternative term used for suicidal erythrocyte death. In the current study, eryptotic effect of naproxen sodium characterized by membrane blebbing was investigated in erythrocytes after 48 hours of treatment with different concentrations (1-25 µM). The experimental work related to investigation of eryptosis was done by cell size measurement and confirmation of calcium role in the induction of membrane blebbing. As a possible mechanism of eryptosis, oxidative stress induced by naproxen sodium was determined by catalase, glutathione peroxidase, and superoxide dismutase activities. Similarly, hemolytic effect of naproxen sodium was also determined by hemolysis measurement. Results of our study illustrated that the therapeutic doses (10-25 µM) of naproxen sodium induce oxidative stress, confirmed by significant decrease in superoxide dismutase, catalase, and glutathione peroxidase activities that lead to the triggering of cell death by eryptosis and hemolysis.
- Published
- 2020
- Full Text
- View/download PDF
10. Atorvastatin Induced Erythrocytes Membrane Blebbing
- Author
-
Rumaisa Bashir Rana, Kashif Jilani, Muhammad Shahid, Muhammad Riaz, Mazhar Hussain Ranjha, Ismat Bibi, Asma Asghar, and Muhammad Irfan
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Atorvastatin, an inhibitor of 3-hydroxy-3-methylglutaryl-coenzymeA reductase, is usually used for the treatment of hypercholesterolemia. Besides its pharmacological and side actions, its toxic effects on human nucleus devoid of erythrocytes are still unknown. Eryptosis is an alternative term used for suicidal erythrocyte death. Membrane blebbing is among the common markers of eryptosis. In this study, eryptotic effect of atorvastatin was investigated by exposing the erythrocytes for 48 hours to different concentrations (1-10 µM) of atorvastatin. The experimental work related to investigation of eryptosis was done by cell size measurement and calcium channel inhibition. As a possible mechanism of eryptosis, atorvastatin-induced oxidative stress was evaluated by determining catalase, glutathione peroxidase, and superoxide dismutase activities. Similarly, necrotic effect of atorvastatin was also determined by hemolytic assay. Results of our study illustrated that the tested doses of atorvastatin may induce oxidative stress as observed by significant reduction in superoxide dismutase, glutathione peroxidase, and catalase activities as well as induce eryptosis, featured by erythrocytes membrane blebbing. The study concluded that induction of oxidative stress by atorvastatin may lead to eryptosis.
- Published
- 2019
- Full Text
- View/download PDF
11. Vitamin D-Rich Diet in Mice Modulates Erythrocyte Survival
- Author
-
Elisabeth Lang, Kashif Jilani, Rosi Bissinger, Rexhep Rexhepaj, Christine Zelenak, Adrian Lupescu, Florian Lang, and Syed M. Qadri
- Subjects
Energy depletion ,Vitamin D ,Eryptosis ,Hyperosmotic shock ,Cell shrinkage ,Dermatology ,RL1-803 ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Background/Aims: Epidemiological evidence suggests that vitamin D deficiency is associated with anemia. The potent metabolite 1,25(OH)2 vitamin D3 [1,25(OH)2D3] activates various signaling cascades regulating a myriad of cellular functions including suicidal cell death or apoptosis. Suicidal death of erythrocytes or eryptosis is characterized by cell shrinkage and cell membrane scrambling leading to phosphatidylserine (PS) externalization. Stimulation of eryptosis may limit lifespan of circulating erythrocytes and thus cause anemia. In the present study, we explored the effect of a high vitamin D diet (10,000 I.U. vitamin D for 14 days) in mice on eryptosis. Methods: Plasma concentrations of erythropoietin were estimated using an immunoassay kit, blood count using an electronic hematology particle counter, relative reticulocyte numbers using Retic-COUNT® reagent, PS exposure at the cell surface from annexin V binding, cell volume from forward scatter, and cytosolic Ca2+ ([Ca2+]i) from Fluo3-fluorescence in FACS analysis. Results: Vitamin D treatment decreased mean corpuscular volume, reticulocyte count, and plasma erythropoietin levels. Vitamin D treatment slightly but significantly decreased forward scatter but did not significantly modify spontaneous PS exposure and [Ca2+]i of freshly drawn erythrocytes. Vitamin D treatment augmented the stimulation of PS exposure and cell shrinkage following exposure to hyperosmotic shock (addition of 550 mM sucrose) or energy depletion (glucose removal) without significantly modifying [Ca2+]i. Conclusions: The present observations point to a subtle effect of exogenous vitamin D supplementation on erythrocyte survival.
- Published
- 2015
- Full Text
- View/download PDF
12. The Role of Janus Kinase 3 in the Regulation of Na+/K+ ATPase under Energy Depletion
- Author
-
Zohreh Hosseinzadeh, Sabina Honisch, Evi Schmid, Kashif Jilani, Kalina Szteyn, Shefalee Bhavsar, Yogesh Singh, Monica Palmada, Anja T. Umbach, Ekaterina Shumilina, and Florian Lang
- Subjects
K+-current ,Ouabain ,DNP ,ATP ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Janus kinase-3 (JAK3) is activated during energy depletion. Energy-consuming pumps include the Na+/K+-ATPase. The present study explored whether JAK3 regulates Na+/K+-ATPase in dendritic cells (DCs). Methods: Ouabain (100 µM)-sensitive (Iouabain) and K+-induced (Ipump) outward currents were determined by utilizing whole cell patch-clamp, Na+/K+-ATPase α1-subunit mRNA levels by RT-PCR, Na+/K+-ATPase protein abundance by flow cytometry or immunofluorescence, and cellular ATP by luciferase-assay in DCs from bone marrow of JAK3-knockout (jak3-/-) or wild-type mice (jak3+/+). Ipump was further determined by voltage clamp in Xenopus oocytes expressing JAK3, active A568VJAK3 or inactive K851AJAK3. Results: Na+/K+-ATPase α1-subunit mRNA and protein levels, as well as Ipump and Iouabain were significantly higher in jak3-/-DCs than in jak3+/+DCs. Energy depletion by 4h pre-treatment with 2,4-dinitro-phenol significantly decreased Ipump in jak3+/+ DCs but not in jak3-/-DCs. Cellular ATP was significantly lower in jak3-/-DCs than in jak3+/+DCs and decreased in both genotypes by 2,4-dinitro-phenol, an effect significantly more pronounced in jak3-/-DCs than in jak3+/+DCs and strongly blunted by ouabain in both jak3+/+ and jak3-/-DCs. Ipump and Iouabain in oocytes were decreased by expression of JAK3 and of A568VJAK3 but not of K851AJAK3. JAK3 inhibitor WHI-P154 (4-[(3'-bromo-4'-hydroxyphenyl)amino]-6,7-dimethoxyquinazoline, 22 μM) enhanced Ipump and Iouabain in JAK3 expressing oocytes. The difference between A568VJAK3 and K851AJAK3 expressing oocytes was virtually abrogated by actinomycin D (50 nM). Conclusions: JAK3 down-regulates Na+/K+-ATPase activity, an effect involving gene expression and profoundly curtailing ATP consumption.
- Published
- 2015
- Full Text
- View/download PDF
13. Stimulation of Erythrocyte Death by Phloretin
- Author
-
Rosi Bissinger, Salome Fischer, Kashif Jilani, and Florian Lang
- Subjects
Phosphatidylserine ,Phloretin ,Calcium ,Cell volume ,Eryptosis ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background: Phloretin, a natural component of apples, pears and strawberries, has previously been shown to stimulate apoptosis of nucleated cells. Erythrocytes may similarly enter suicidal death or eryptosis, which is characterized by cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane with phosphatidylserine translocation to the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i), ceramide, ATP depletion, and activation of protein kinase C (PKC) as well as p38 mitogen activated protein kinase (p38 kinase). Methods: Phosphatidylserine exposure at the cell surface was estimated from annexin V binding, cell volume from forward scatter, [Ca2+]i from Fluo3-fluorescence, and ceramide abundance from binding of specific antibodies. Results: A 48 h exposure of human erythrocytes to phloretin significantly increased the percentage of annexin-V-binding cells (≥100 µM) without significantly influencing forward scatter. Phloretin did not significantly modify [Ca2+]i and the stimulation of annexin-V-binding by phloretin (300 µM) did not require presence of extracellular Ca2+. Phloretin did not significantly modify erythrocyte ATP levels, and the effect of phloretin on annexin-V-binding was not significantly altered by PKC inhibitor staurosporine (1 µM) or p38 kinase inhibitor SB2203580 (2 µM). However, phloretin significantly increased the ceramide abundance at the cell surface. Conclusions: Phloretin stimulates phospholipid scrambling of the erythrocyte cell membrane, an effect at least partially due to up-regulation of ceramide abundance.
- Published
- 2014
- Full Text
- View/download PDF
14. Aristolochic Acid Induced Suicidal Erythrocyte Death
- Author
-
Abaid Malik, Rosi Bissinger, Salvatrice Calabrò, Caterina Faggio, Kashif Jilani, and Florian Lang
- Subjects
Phosphatidylserine ,Aristolochic Acid ,Calcium ,Ceramide ,Cell volume ,Eryptosis ,Dermatology ,RL1-803 ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Background/Aims: Aristolochic Acid, a component of Aristolochia plants, has been shown to cause acute kidney injury, renal aristolochic acid nephropathy, Balkan endemic nephropathy, and urothelial carcinoma. Aristolochic acid nephropathy may be associated with severe anemia. The anemia could theoretically be due to stimulation of eryptosis, the suicidal death of erythrocytes characterized by cell shrinkage and cell membrane scrambling with translocation of phosphatidylserine to the erythrocyte cell membrane surface. Signalling involved in the stimulation of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i) and formation of ceramide. Methods: Cell volume was estimated from forward scatter, phosphatidylserine-exposure from annexin V binding, [Ca2+]i from Fluo3 fluorescence, and ceramide abundance from binding of fluorescent antibodies in flow cytometry. Results: A 48 hours exposure to Aristolochic Acid (≥ 75 g/ml) was followed by a significant decrease of forward scatter and increase of annexin-V-binding. The effects were paralleled by a significant increase of [Ca2+]i and significantly blunted, but not abrogated by removal of extracellular Ca2+. Aristolochic Acid further significantly increased ceramide abundance. Conclusions: Aristolochic Acid triggers eryptosis, an effect at least in part due to entry of extracellular Ca2+ and ceramide formation. i 2014 S. Karger AG, Basel
- Published
- 2014
- Full Text
- View/download PDF
15. Piperlongumine-Induced Phosphatidylserine Translocation in the Erythrocyte Membrane
- Author
-
Rosi Bissinger, Abaid Malik, Jamshed Warsi, Kashif Jilani, and Florian Lang
- Subjects
phosphatidylserine ,piperlongumine ,calcium ,ceramide ,cell volume ,eryptosis ,Medicine - Abstract
Background: Piperlongumine, a component of Piper longum fruit, is considered as a treatment for malignancy. It is effective by inducing apoptosis. Mechanisms involved in the apoptotic action of piperlongumine include oxidative stress and activation of p38 kinase. In analogy to apoptosis of nucleated cells, erythrocytes may undergo eryptosis, the suicidal death of erythrocytes characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine-exposure at the erythrocyte surface. Signaling involved in eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i), formation of ceramide, oxidative stress and activation of p38 kinase. Methods: Cell volume was estimated from forward scatter, phosphatidylserine-exposure from annexin V binding, [Ca2+]i from Fluo3 fluorescence, reactive oxygen species from 2',7'-dichlorodihydrofluorescein-diacetate fluorescence, and ceramide abundance from binding of fluorescent antibodies in flow cytometry. Results: A 48 h exposure to piperlongumine (30 µM) was followed by significant decrease of forward scatter and increase of annexin-V-binding. Piperlongumine did not significantly modify [Ca2+]i and the effect was not dependent on presence of extracellular Ca2+. Piperlongumine significantly increased ROS formation and ceramide abundance. Conclusions: Piperlongumine triggers cell membrane scrambling, an effect independent from entry of extracellular Ca2+ but at least partially due to ROS and ceramide formation.
- Published
- 2014
- Full Text
- View/download PDF
16. In Vitro Sensitization of Erythrocytes to Programmed Cell Death Following Baicalein Treatment
- Author
-
Rosi Bissinger, Abaid Malik, Sabina Honisch, Jamshed Warsi, Kashif Jilani, and Florian Lang
- Subjects
phosphatidylserine ,Baicalein ,Calcium ,cell volume ,ceramide ,eryptosis ,Medicine - Abstract
The polyphenolic flavonoid Baicalein has been shown to trigger suicidal death or apoptosis of tumor cells and is thus considered for the prevention and treatment of malignancy. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i) and ceramide. The present study explored whether Baicalein stimulates eryptosis. To this end, forward scatter was taken for measurement of cell volume, annexin-V-binding for phosphatidylserine-exposure, Fluo3 fluorescence for [Ca2+]i and fluorescent antibodies for ceramide abundance. As a result, a 48 h exposure of human erythrocytes to Baicalein was followed by significant decrease of forward scatter (≥10 µM), significant increase of the percentage of annexin-V-binding cells (≥25 µM), significant increase of [Ca2+]i (50 µM) and significant increase of ceramide abundance (50 µM). The effect of Baicalein (50 µM) on annexin-V-binding was significantly blunted but not abrogated by removal of extracellular Ca2+. In conclusion, at the concentrations employed, Baicalein stimulates suicidal erythrocyte death or eryptosis, an effect at least in part due to the combined effects of Ca2+ entry and ceramide formation.
- Published
- 2014
- Full Text
- View/download PDF
17. Stimulation of Eryptosis by Cryptotanshinone
- Author
-
Rosi Bissinger, Adrian Lupescu, Christine Zelenak, Kashif Jilani, and Florian Lang
- Subjects
Phosphatidylserine ,Cryptotanshinone ,Calcium ,Cell volume ,Eryptosis ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Cryptotanshinone, a component of Salvia miltiorrhiza Bunge roots, may trigger suicidal death or apoptosis of tumor cells and has thus been recommended for the prevention and treatment of malignancy. On the other hand, Cryptotanshinone has been shown to counteract apoptosis of neurons and hepatocytes. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and phosphatidylserine translocation to the erythrocyte surface. Eryptosis may be triggered by increase of cytosolic Ca2+-activity ([Ca2+]i). The present study explored whether Cryptotanshinone stimulates eryptosis. Methods: Forward scatter was taken as measure of cell volume, annexin V binding for identification of phosphatidylserine-exposing erythrocytes and Fluo3-fluorescence for determination of [Ca2+]i. Results: A 48 h exposure of human erythrocytes to Cryptotanshinone (10 µM) was followed by significant decrease of forward scatter, significant increase of the percentage annexin-V-binding cells and significant increase of [Ca2+]i. The effect of Cryptotanshinone (1 µM) on annexin-V-binding was virtually abrogated by removal of extracellular Ca2+. Conclusion: Cryptotanshinone is a powerful stimulator of suicidal erythrocyte death or eryptosis, which is effective mainly, if not exclusively, by stimulation of Ca2+ entry.
- Published
- 2014
- Full Text
- View/download PDF
18. Stimulation of Erythrocyte Cell Membrane Scrambling by Gedunin
- Author
-
Adrian Lupescu, Rosi Bissinger, Jamshed Warsi, Kashif Jilani, and Florian Lang
- Subjects
Phosphatidylserine ,Gedunin ,Calcium ,Malaria ,Anemia ,Cell volume ,Eryptosis ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Gedunin, an inhibitor of heat shock protein HSP90, triggers apoptosis of tumor cells and is thus effective against malignancy. Moreover, the drug has antimalarial potency. In analogy to apoptosis of nucleated cells, erythrocytes may enter suicidal death or eryptosis, which is characterized by cell shrinkage and by phosphatidylserine translocation to the erythrocyte surface. Eryptosis may be triggered by increase of cytosolic Ca2+-activity ([Ca2+]i). The present study explored whether gedunin stimulates eryptosis. Methods: Forward scatter was determined to estimate cell volume, annexin V binding to identify phosphatidylserine-exposing erythrocytes, hemoglobin release to depict hemolysis, and Fluo3-fluorescence to quantify [Ca2+]i. Results: A 48 h exposure of human erythrocytes to gedunin significantly increased [Ca2+]i (12 µM), significantly decreased forward scatter (24 µM) and significantly increased annexin-V-binding (12 µM). The effect of gedunin (24 µM) on annexin-V-binding was virtually abrogated by removal of extracellular Ca2+. Conclusion: Gedunin stimulates suicidal erythrocyte death or eryptosis, an effect mainly if not exclusively due to stimulation of Ca2+ entry.
- Published
- 2014
- Full Text
- View/download PDF
19. In Vitro Induction of Erythrocyte Phosphatidylserine Translocation by the Natural Naphthoquinone Shikonin
- Author
-
Adrian Lupescu, Rosi Bissinger, Kashif Jilani, and Florian Lang
- Subjects
phosphatidylserine ,shikonin ,calcium ,ceramide ,cell volume ,eryptosis ,Medicine - Abstract
Shikonin, the most important component of Lithospermum erythrorhizon, has previously been shown to exert antioxidant, anti-inflammatory, antithrombotic, antiviral, antimicrobial and anticancer effects. The anticancer effect has been attributed to the stimulation of suicidal cell death or apoptosis. Similar to the apoptosis of nucleated cells, erythrocytes may experience eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and by phosphatidylserine translocation to the erythrocyte surface. Triggers of eryptosis include the increase of cytosolic Ca2+-activity ([Ca2+]i) and ceramide formation. The present study explored whether Shikonin stimulates eryptosis. To this end, Fluo 3 fluorescence was measured to quantify [Ca2+]i, forward scatter to estimate cell volume, annexin V binding to identify phosphatidylserine-exposing erythrocytes, hemoglobin release to determine hemolysis and antibodies to quantify ceramide abundance. As a result, a 48 h exposure of human erythrocytes to Shikonin (1 µM) significantly increased [Ca2+]i, increased ceramide abundance, decreased forward scatter and increased annexin V binding. The effect of Shikonin (1 µM) on annexin V binding was significantly blunted, but not abolished by the removal of extracellular Ca2+. In conclusion, Shikonin stimulates suicidal erythrocyte death or eryptosis, an effect at least partially due to the stimulation of Ca2+ entry and ceramide formation.
- Published
- 2014
- Full Text
- View/download PDF
20. Induction of Suicidal Erythrocyte Death by Novobiocin
- Author
-
Adrian Lupescu, Rosi Bissinger, Tabea Herrmann, Gergely Oswald, Kashif Jilani, and Florian Lang
- Subjects
Phosphatidylserine ,Calcium ,Cell volume ,Eryptosis ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background: Novobiocin, an aminocoumarin antibiotic, interferes with heat shock protein 90 and hypoxia inducible factor dependent gene expression and thus compromises cell survival. Similar to survival of nucleated cells, erythrocyte survival could be disrupted by eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and by phospholipd scrambling of the cell membrane with phosphatidylserine translocation to the erythrocyte surface. Triggers of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i). The Ca2+ sensitivity of phospholipid scrambling is enhanced by ceramide. The present study explored, whether novobiocin elicits eryptosis. Methods: [Ca2+]i was estimated from Fluo3-fluorescence, ceramide abundance utilizing fluorescent antibodies, cell volume from forward scatter, phosphatidylserine-exposure from annexin V binding. Results: A 48 hours exposure to novobiocin (500 µM) was followed by a significant increase of [Ca2+]i, decrease of forward scatter, increase of annexin-V-binding and enhanced ceramide formation. Removal of extracellular Ca2+ virtually abrogated the increase of annexin-V-binding following novobiocin exposure. Conclusions: Novobiocin stimulates eryptosis, an effect at least in part due to entry of extracellular Ca2+ and formation of ceramide.
- Published
- 2014
- Full Text
- View/download PDF
21. Geldanamycin-Induced Phosphatidylserine Translocation in the Erythrocyte Membrane
- Author
-
Kashif Jilani, Syed M. Qadri, and Florian Lang
- Subjects
Phosphatidylserine ,Geldanamycin ,Calcium ,Ceramide ,Cell volume ,Eryptosis ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/aims: Geldanamycin, a benzoquinone ansamycin antibiotic, and its analogues induce apoptosis of tumor cells and are thus considered for the treatment of cancer. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and by cell membrane scrambling with phosphatidylserine-exposure at the erythrocyte surface. Triggers of eryptosis include increase of cytosolic Ca2+-concentration ([Ca2+]i) and formation of ceramide. The present study explored, whether geldanamycin modifies [Ca2+]i, ceramide formation, cell volume and phosphatidylserine abundance at the erythrocyte surface. Methods: Erythrocyte volume was estimated from forward scatter, phosphatidylserine-abundance from annexin V binding, hemolysis from hemoglobin release, ceramide formation from binding of fluorescent antibodies and [Ca2+]i from Fluo3-fluorescence. Results: A 48 hours exposure to geldanamycin significantly decreased forward scatter (≥ 5 µM), significantly increased annexin-V-binding (≥ 25 µM), but did not significantly modify Fluo3-fluorescence (up to 50 µM). The annexin-V-binding following geldanamycin treatment was not significantly modified by removal of extracellular Ca2+ but was paralleled by significantly increased ceramide formation (50 µM). Conclusions: Geldanamycin stinulated eryptosis, an effect at least partially due to ceramide formation.
- Published
- 2013
- Full Text
- View/download PDF
22. Triggering of Suicidal Erythrocyte Death by Celecoxib
- Author
-
Florian Lang, Kashif Jilani, Rosi Bissinger, and Adrian Lupescu
- Subjects
cell membrane scrambling ,phosphatidylserine ,calcium ,cell volume ,eryptosis ,Medicine - Abstract
The selective cyclooxygenase-2 (COX-2) inhibitor celecoxib triggers apoptosis of tumor cells and is thus effective against malignancy. The substance is at least partially effective through mitochondrial depolarization. Even though lacking mitochondria, erythrocytes may enter apoptosis-like suicidal death or eryptosis, which is characterized by cell shrinkage and by phosphatidylserine translocation to the erythrocyte surface. Eryptosis may be triggered by increase of cytosolic Ca2+-activity ([Ca2+]i). The present study explored whether celecoxib stimulates eryptosis. Forward scatter was determined to estimate cell volume, annexin V binding to identify phosphatidylserine-exposing erythrocytes, hemoglobin release to depict hemolysis, and Fluo3-fluorescence to quantify [Ca2+]i. A 48 h exposure of human erythrocytes to celecoxib was followed by significant increase of [Ca2+]i (15 µM), significant decrease of forward scatter (15 µM) and significant increase of annexin-V-binding (10 µM). Celecoxib (15 µM) induced annexin-V-binding was blunted but not abrogated by removal of extracellular Ca2+. In conclusion, celecoxib stimulates suicidal erythrocyte death or eryptosis, an effect partially due to stimulation of Ca2+ entry.
- Published
- 2013
- Full Text
- View/download PDF
23. Patulin-Induced Suicidal Erythrocyte Death
- Author
-
Adrian Lupescu, Kashif Jilani, Mohanad Zbidah, and Florian Lang
- Subjects
Phosphatidylserine ,Patulin ,Calcium ,Cell volume ,Eryptosis ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background: Patulin, the most common mycotoxin in apples and apple-derived products, triggers apoptosis and has thus been considered for the treatment of cancer. Similar to apoptosis of nucleated cells, erythrocytes may enter suicidal death or eryptosis, which is characterized by cell shrinkage and by cell membrane scrambling leading to phosphatidylserine-exposure at the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+-activity ([Ca2+]i). The present study explored, whether exposure of human erythrocytes to patulin is followed by eryptosis. Methods: Forward scatter was measured to estimate cell volume, annexin V binding to detect phosphatidylserine-exposure, hemoglobin release to quantify hemolysis, and Fluo3-fuorescence to determine [Ca2+]i. Results: A 48 h exposure to patulin significantly increased [Ca2+]I (5 µM), significantly decreased forward scatter (5 µM) and significantly increased annexin-V-binding (2.5 µM). Patulin (10 µM) induced annexin-V-binding was virtually abrogated by removal of extracellular Ca2+. Conclusion: Patulin stimulates Ca2+ entry into erythrocytes, an effect triggering suicidal erythrocyte death or eryptosis.
- Published
- 2013
- Full Text
- View/download PDF
24. Fluoxetine Induced Suicidal Erythrocyte Death
- Author
-
Florian Lang, Majed Abed, Ahmad Almilaji, Rosi Bissinger, Sigrid Enkel, and Kashif Jilani
- Subjects
phosphatidylserine ,fluoxetine ,calcium ,cell volume ,eryptosis ,Medicine - Abstract
The antidepressant fluoxetine inhibits ceramide producing acid sphingomyelinase. Ceramide is in turn known to trigger eryptosis the suicidal death of erythrocytes characterized by cell shrinkage and exposure of phosphatidylserine at the erythrocyte surface. Ceramide is effective through sensitizing the erythrocytes to the pro-eryptotic effect of increased cytosolic Ca2+ activity ([Ca2+]i). In nucleated cells, fluoxetine could either inhibit or stimulate suicidal death or apoptosis. The present study tested whether fluoxetine influences eryptosis. To this end cell volume was estimated from forward scatter, phosphatidylserine exposure from annexin V binding, hemolysis from hemoglobin release and [Ca2+]i from Fluo-3 fluorescence intensity. As a result, a 48 h exposure of erythrocytes to fluoxetine (≥25 µM) significantly decreased forward scatter, increased annexin V binding and enhanced [Ca2+]i. The effect on annexin V binding was significantly blunted, but not abolished, in the absence of extracellular Ca2+. In conclusion, fluoxetine stimulates eryptosis, an effect at least in part due to increase of cytosolic Ca2+ activity.
- Published
- 2013
- Full Text
- View/download PDF
25. Carmustine-Induced Phosphatidylserine Translocation in the Erythrocyte Membrane
- Author
-
Kashif Jilani and Florian Lang
- Subjects
phosphatidylserine ,carmustine ,calcium ,cell volume ,eryptosis ,Medicine - Abstract
The nitrosourea alkylating agent, carmustine, is used as chemotherapeutic drug in several malignancies. The substance triggers tumor cell apoptosis. Side effects of carmustine include myelotoxicity with anemia. At least in theory, anemia could partly be due to stimulation of eryptosis, the suicidal death of erythrocytes, characterized by cell shrinkage and breakdown of phosphatidylserine asymmetry of the cell membrane with phosphatidylserine exposure at the erythrocyte surface. Stimulators of eryptosis include increase of cytosolic Ca2+ activity ([Ca2+]i). The present study tested whether carmustine triggers eryptosis. To this end [Ca2+]i was estimated from Fluo3 fluorescence, cell volume from forward scatter, phosphatidylserine exposure from annexin V binding, and hemolysis from hemoglobin release. As a result a 48 h exposure to carmustine (≥25 µM) significantly increased [Ca2+]i, decreased forward scatter and increased annexin V binding. The effect on annexin V binding was significantly blunted in the absence of extracellular Ca2+. In conclusion, carmustine stimulates eryptosis at least partially by increasing cytosolic Ca2+ activity.
- Published
- 2013
- Full Text
- View/download PDF
26. Green synthesis of iron nanoparticles and photocatalytic activity evaluation for the degradation of methylene blue dye
- Author
-
Ismat Bibi, Tahir Ghulam, Shagufta Kamal, Kashif Jilani, Norah Alwadai, and Munawar Iqbal
- Subjects
Physical and Theoretical Chemistry - Abstract
The present study focuses on iron nanoparticles (Fe NPs) biosynthesis, characterization and photocatalytic activity (PCA) appraisal for methylene blue dye degradation. A green rapid biogenic synthesis route was employed for synthesis of Fe NPs using banana peel extract. The synthesized Fe NPs was characterized by XRD (X-ray diffraction), SEM (scanning electron microscopy) and EDX (energy dispersive X-ray) techniques. These analysis confirmed the synthesis of zero valent Fe NPs with high crystallinity, purity and semi spherical in shape. The photocatalytic activity was assessed under ultra violet irradiation and under optimum conditions, 87% degradation of MB dye was obtained for 72 min of irradiation, which revealed promising catalytic efficiency of the Fe NPs. The result shows that photocatalytic activity of Fe NPs is promising and could possibly be used to treat dyes in industrial effluents and the use of green synthetic protocol is suggested due to its ecofriendly nature.
- Published
- 2022
- Full Text
- View/download PDF
27. Efficient label-free detection of chloramphenicol by iron-doped cadmium sulfide nanomaterials
- Author
-
Gulzar Ahmad, Yasir Javed, Yasir Jamil, and Kashif Jilani
- Subjects
Electrical and Electronic Engineering ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials - Published
- 2022
- Full Text
- View/download PDF
28. The electrochemical, dielectric, and ferroelectric properties of Gd and Fe doped LaNiO3 with an efficient solar-light driven catalytic activity to oxidize malachite green dye
- Author
-
Shagufta Kamal, Hind Albalawi, Farzana Majid, Sadia Ata, Kashif Jilani, Ismat Bibi, Nosheen Nazar, Munawar Iqbal, Shahid Iqbal, and Norah Alwadai
- Subjects
Materials science ,Dopant ,Doping ,Dielectric ,Conductivity ,Coercivity ,Ferroelectricity ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Colloid and Surface Chemistry ,Chemical engineering ,Photocatalysis ,Fourier transform infrared spectroscopy - Abstract
This work investigates the effects of double ion substitution on the ferroelectric, electrochemical, dielectric and photocatalytic properties of Gd and Fe doped La1-yGdyNi1-xFexO3 nanoparticles (NPs). La1-yGdyNi1-xFexO3 was fabricated by facile micro-emulsion path and its properties were studied by thermogravimetric analysis (TGA), X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman scattering, Fourier Transform of Infrared (FTIR), energy dispersive x-rays (EDX) techniques. It has a distorted rhombohedral shape with crystallite size within the range of 17–23 nm. The doped material has a spherical heterogeneous morphology, and its surface area increased with increased doping. The electrochemical (CV, EIS, and I-V), conductivity and dielectric (dielectric constant and low dielectric & tangent loss) properties of La1-yGdyNi1-xFexO3 were dependent on the contents of the dopants (Gd and Fe). The doped material had improved specific capacitance compared to the undoped LaNiO3 due to the synergistic effect of Gd and Fe on the doped materials. The conductivity of Gd and Fe doped LaNiO3 5.16 × 104 Sm−1 was enhanced compared to the undoped LaNiO3 3.52 × 10−2 Sm1. Furthermore, hysteresis loop was used to investigate the coercivity (Hc), saturation magnetization (Ms) and remanence (Mr) of the material. The Ms and Mr values were enhanced with the content of the dopants. The photocatalytic activity (PCA) of the material in degrading malachite green (MG) dye was studied. La1-yGdyNi1-xFexO3 NPs was able to degrade up to 96.4% of the dye under visible light irradiation in 50 min. La1-yGdyNi1-xFexO3 has remarkable dielectric, electrochemical, ferroelectric and photo-catalytic properties and have potential applications in microwave, electrical, electronic, energy storage devices. It is also an active photo-catalyst material for the removal/oxidation of toxic pollutants from the environment.
- Published
- 2022
- Full Text
- View/download PDF
29. Synthesis of Silver Nanoparticle from Allium sativum as an Eco-Benign Agent for Biological Applications
- Author
-
Mazhar Abbas, Tariq Hussain, Javed Iqbal, Aziz Rehman, Muhammad Arfan Zaman, Kashif Jilani, Nasir Masood, Samiah H. Al-Mijalli, Munawar Iqbal, and Arif Nazir
- Subjects
Environmental Chemistry ,General Environmental Science - Published
- 2021
- Full Text
- View/download PDF
30. Pathogenic role of cytokines in COVID-19, its association with contributing co-morbidities and possible therapeutic regimens
- Author
-
Ayesha Tanveer, Bushra Akhtar, Ali Sharif, Uzma Saleem, Azhar Rasul, Aftab Ahmad, and Kashif Jilani
- Subjects
Pharmacology ,Pulmonary Disease, Chronic Obstructive ,SARS-CoV-2 ,Immunology ,Cytokines ,Humans ,Pharmacology (medical) ,Comorbidity ,Cytokine Release Syndrome ,Inflammatory Bowel Diseases ,Bronchodilator Agents ,COVID-19 Drug Treatment - Abstract
The Covid-19, a threatening pandemic, was originated from China in December 2019 and spread quickly to all over the world. The pathogenesis of coronavirus is linked with the disproportionate response of the immune system. This involves the systemic inflammatory reaction which is characterized by marked pro-inflammatory cytokine release commonly known as cytokine release storm (CRS). The pro inflammatory cytokines are involved in cascade of pulmonary inflammation, hyper coagulation and thrombosis which may be lethal for the individual. That's why, it is very important to have understanding of pro inflammatory cytokines and their pathological role in SARS-CoV-2. The pathogenesis of Covid is not the same in every individual, it can vary due to the presence of pre-existing comorbidities like suffering from already an inflammatory disease such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD), chronic obstructive pulmonary disease (COPD), an immune-compromised patients suffering from Diabetes Mellitus (DM) and Tuberculosis (TB) are more vulnerable morbidity and complications following COVID-19. This review is particularly related to COVID-19 patients having comorbidity of other inflammatory diseases. We have discussed the brief pathogenesis of COVID-19 and cytokines release storm with reference to other co-morbidities including RA, IBD, COPD, DM and TB. The available therapeutic regimens for COVID-19 including cytokine inhibitors, anti-viral, anti-biotic, bronchodilators, JAK- inhibitors, immunomodulators and anti-fibrotic agents have also been discussed briefly. Moreover, newly emerging medicines in the clinical trials have also been discussed which are found to be effective in treating Covid-19.
- Published
- 2022
31. Ferroelectric, dielectric, magnetic, structural and photocatalytic properties of Co and Fe doped LaCrO3 perovskite synthesized via micro-emulsion route
- Author
-
Farzana Majid, Shagufta Kamal, M.A.S. Raza, Sadia Ata, Shahid Iqbal, Ismat Bibi, Muhammad Aadil, Muhammad Aamir, Muzaffar Bashir, Kashif Jilani, Norah Alwadai, and Munawar Iqbal
- Subjects
010302 applied physics ,Materials science ,Dopant ,Process Chemistry and Technology ,Doping ,02 engineering and technology ,Dielectric ,021001 nanoscience & nanotechnology ,01 natural sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Reaction rate constant ,chemistry ,0103 physical sciences ,Materials Chemistry ,Ceramics and Composites ,Photocatalysis ,Crystal violet ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Nuclear chemistry ,Perovskite (structure) - Abstract
In the present investigation, La1-xCoxCr1-yFeyO3 (x,y = 0.0, 0.12, 0.36, 0.60) perovskite was fabricated via a facile micro-emulsion route. The synthesized perovskites were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) techniques to examine the effect of Co and Fe ions on the physico-chemical properties. The ferroelectric, dielectric, and magnetic properties of La1-xCoxCr1-yFeyO3 were changed significantly as a function of dopants contents (Co and Fe ions). Outcomes revealed that the dielectric, ferroelectric and magnetic properties of LaCrO3 perovskite can be tuned significantly via Co and Fe doping and La0.40Co0.60Cr0.40Fe0.60O3 have potential for photocatalytic dye removal under (visible) light expoure. The photocatalytic activity (PCA) of the pristine LaCrO3 and La0.40Co0.60Cr0.40Fe0.60O3 photocatalyst was evaluated under (visible) light irradiation for crystal violet (CV) dye. Experimental results revealed that La0.40Co0.60Cr0.40Fe0.60O3 photocatalyst degrdae almost 77.21% CV dye with the rate constant value of 0.01475 min−1. In the presence of isopropyl alcohol (IPA) scavenger, the PCA of the La0.40Co0.60Cr0.40Fe0.60O3 photocatalyst and rate constant value of the photocatalytic reaction decreased to 32.5% and 0.00491 min−1, suggesting the superoxide as main active specie. Results revealed that Co and Fe doping doped material is efficient for photocatalytic presentations under solar light expoure.
- Published
- 2021
- Full Text
- View/download PDF
32. Electrochemical, ferroelectric and dielectric properties of Gd, Fe and Mn doped nickel perovskite with robust photocatalytic activity under visible light
- Author
-
Aamir Ghafoor, Ismat Bibi, Farzana Majid, Shagufta Kamal, Kashif Jilani, Misbah Sultan, Norah Alwadai, Adnan Ali, Abid Ali, Arif Nazir, and Munawar Iqbal
- Subjects
Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Condensed Matter Physics - Published
- 2023
- Full Text
- View/download PDF
33. Hydrothermal route for the synthesis of manganese ferrite nanoparticles and photocatalytic activity evaluation for the degradation of methylene blue dye
- Author
-
Ismat Bibi, Farzana Majid, Kashif Jilani, Munawar Iqbal, Yassine Slimani, Ijaz-ul-Mohsin, Sadia Ata, and Ifra Shaheen
- Subjects
Materials science ,Nanoparticle ,02 engineering and technology ,Manganese ferrite ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Hydrothermal circulation ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Photocatalysis ,Degradation (geology) ,Physical and Theoretical Chemistry ,0210 nano-technology ,Methylene blue ,Nuclear chemistry - Abstract
Manganese ferrite (MnFe2O4) was prepared via hydrothermal route and characterized by advanced techniques. The photocatalytic activity (PCA) was evaluated by degrading methylene blue (MB) dye under UV irradiation. The effect of process variables such as catalyst dose, UV exposure time and pH was studied for maximum degradation of dye at optimum conditions. The MnFe2O4 showed face centered cubic structure and average particle size of 23.98 nm. The lattice constant, lattice strain, ionic radii (rA & rB), bonding angles and hoping lengths of MnFe2O4 were recorded to be 0.8467 nm, 0.08, 1.66, 0.766, 1.833 and 2.116 Å, respectively. The MnFe2O4 showed promising PCA and at optimum conditions of process variable, up to 99% MB dye degradation was achieved. The PCA was found dependent to catalyst dose, UV exposure time and pH. Results revealed that the hydrothermal rout is feasible route for the preparation of MnFe2O4 ferrite in nano size and the PCA revealed the potential application of MnFe2O4 ferrite to degrade dye in textile wastewater.
- Published
- 2021
- Full Text
- View/download PDF
34. Enhanced Dielectric, Electrochemical, Conductivity, Ferroelectric and Photocatalytic Properties of Gd and Fe Doped Lanio3 Synthesized Via Microemulsion Approach
- Author
-
Aamir Ghafoor, Ismat Bibi, Farzana Majid, Kashif Jilani, Shagufta Kamal, Misbah Sultan, Nosheen Nazar, and Munawar Iqbal
- Published
- 2022
- Full Text
- View/download PDF
35. Stimulation of Erythrocyte Membrane Blebbing by Bifenthrin Induced Oxidative Stress
- Author
-
Fatima Mukhtar, Kashif Jilani, Ismat Bibi, Zahid Mushtaq, Maham Abdul Bari Khan, and Maria Fatima
- Subjects
Chemical Health and Safety ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Toxicology - Abstract
Background Bifenthrin is an insecticide and anti-estrogenic compound primarily used to control residential pests by depolarizing sodium gated voltage channels in the nervous system. Eryptosis, the suicidal death of erythrocytes, featured by PS exposure, membrane blebbing and cell shrinkage. Anemia is an outcome of uncontrolled eryptosis. Research Design In this study, erythrocytes were treated with different concentrations (.5-1-1.5 μM) of bifenthrin over a period of 48 hours. In order to investigate the oxidative stress induced by bifenthrin, catalase, superoxide dismutase, and glutathione peroxidase activities were investigated. Results Obtained data indicated the decrease in the enzymes (superoxide dismutase, glutathione peroxidase, and catalase) activities in bifenthrin treated cells at 1 μM concentration. In addition, measurement of cell size and confirmation of the role of calcium in the stimulation of the eryptotic activity of bifenthrin were performed. A significant increase in mean cell volume was found in the presence of bifenthrin and a decrease in mean cell volume in the presence of calcium channel blocker was observed. Similarly, there was also a significant increase in the percentage of hemolysis indicating the necrotic activity of bifenthrin. Conclusions It is concluded that the indicated doses of bifenthrin triggered oxidative stress which may lead to early cell death by eryptosis and hemolysis.
- Published
- 2021
36. Green synthesis of iron oxide nanoparticles using pomegranate seeds extract and photocatalytic activity evaluation for the degradation of textile dye
- Author
-
Fazli Malik Sarim, Shagufta Kamal, Abid Ali, Ismat Bibi, Sadia Ata, Kashif Jilani, Ansar Abbas, Nosheen Nazar, Misbah Sultan, Munawar Iqbal, M. Iftikhar Khan, and Fatima Jalal
- Subjects
lcsh:TN1-997 ,Materials science ,02 engineering and technology ,01 natural sciences ,Biomaterials ,Ferulic acid ,chemistry.chemical_compound ,0103 physical sciences ,Vanillic acid ,Gallic acid ,Methyl gallate ,lcsh:Mining engineering. Metallurgy ,Benzoic acid ,010302 applied physics ,biology ,Metals and Alloys ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Surfaces, Coatings and Films ,chemistry ,Punica ,Ceramics and Composites ,Photocatalysis ,0210 nano-technology ,Iron oxide nanoparticles ,Nuclear chemistry - Abstract
Iron oxide nanoparticles (Fe2O3 NPs) were fabricated through green route using pomegranate (Punica granatum) seeds extract. The Fe2O3 NPs were characterized by UV–vis, XRD, EDX, SEM and AFM techniques. The adopted green rout furnished semi spherical Fe2O3 NPs, uniformly distributed and particle size in the range of 25–55 nm. The LCMS/MS was performed for the identification of biomolecule present in the extract of pomegranate seeds and p-hydroxy benzoic acid, gallic acid, methyl gallate, catechin, kaempferol-3-O-sophoroside, 3-deoxyflavonoids, magnolol, ferulic acid, vanillic acid and pinocembrin along with other minor constituents were detected in the extracts using for Fe2O3 NPs. The synthesized Fe2O3 NPs showed excellent photocatalytic activity against reactive blue under UV light irradiation and maximum degradation of 95.08% was achieved with 56 min of reaction time. In view of promising activity, the Fe2O3 NPs could be used photocatalyst for the degradation of dyes in wastewater and pomegranate seeds extract can be applied as eco-benign and cost effective approach for Fe2O3 NPs synthesis. Keywords: Punica granatum, Green synthesis, Iron oxide nanoparticles, Photocatalysis, Dye degradation
- Published
- 2019
- Full Text
- View/download PDF
37. Triggering of erythrocyte membrane blebbing by ciprofloxacin
- Author
-
Mohanad Zbidah, Muhammad Riaz, Kashif Jilani, Asma Asghar, Ismat Bibi, and Kashif Shabir
- Subjects
Pharmacology ,Ciprofloxacin ,Erythrocyte membrane ,Chemistry ,medicine ,Biophysics ,Pharmaceutical Science ,medicine.drug - Published
- 2019
- Full Text
- View/download PDF
38. Effect of Fe and Bi doping on LaCoO3 structural, magnetic, electric and catalytic properties
- Author
-
Farzana Majid, Abid Ali, Sara Ajmal, Ismat Bibi, Kashif Kamran, Munawar Iqbal, Shagufta Kamal, Shazia Nouren, Sadia Ata, and Kashif Jilani
- Subjects
010302 applied physics ,lcsh:TN1-997 ,Materials science ,Scanning electron microscope ,Doping ,Metals and Alloys ,Analytical chemistry ,02 engineering and technology ,Dielectric ,021001 nanoscience & nanotechnology ,01 natural sciences ,Surfaces, Coatings and Films ,Biomaterials ,symbols.namesake ,Electrical resistivity and conductivity ,0103 physical sciences ,Ceramics and Composites ,Photocatalysis ,symbols ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Raman spectroscopy ,lcsh:Mining engineering. Metallurgy ,Perovskite (structure) - Abstract
La1-xBixCo1-yFeyO3 perovskite was prepared by micro-emulsion method and effect of the Fe and Bi doping on the properties of perovskite was investigated. As-prepared perovskite was characterized by X-ray diffraction (XRD), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), Energy-dispersive X-ray (EDX) and atomic force microscopy (AFM) techniques. La1-xBixCo1-yFeyO3 perovskite showed distorted rhombohedral structure and particle size was in the range of 33.05–57.41 nm. Doping of perovskite with Bi and Fe enhanced the direct current (DC) resistivity. Dielectric parameters were studied in the range of 20 to 20 MHz and vibrating magnetometery revealed that the Mr and Ms values were higher for La0.75Bi0.25Co0.75Fe0.25O3, whereas LaCoO3 showed higher value of Hc. Photocatalytic activity (PCA) was evaluated by degrading congo red dye and Bi and Fe doped perovskite showed significantly higher activity versus LaCoO3. Results revealed that the doping of LaCoO3 with Bi and Fe enhanced the magnetic, dielectric and catalytic properties of LaCoO3. Keywords: LaCoO3 perovskite, Bi and Fe doping, Characterization, Properties, Photocatalysis
- Published
- 2019
39. The electrochemical, dielectric, and ferroelectric properties of Gd and Fe doped LaNiO
- Author
-
Shahid, Iqbal, Ismat, Bibi, Farzana, Majid, Kashif, Jilani, Shagufta, Kamal, Munawar, Iqbal, Sadia, Ata, Nosheen, Nazar, Hind, Albalawi, and Norah, Alwadai
- Subjects
Light ,X-Ray Diffraction ,Rosaniline Dyes ,Catalysis - Abstract
This work investigates the effects of double ion substitution on the ferroelectric, electrochemical, dielectric and photocatalytic properties of Gd and Fe doped La
- Published
- 2021
40. Micro-emulsion synthesis of La1 − xCrxFeO3 nanoparticles: effect of Cr doping on ferroelectric, dielectric and photocatalytic properties
- Author
-
Ismat Bibi, Farzana Majid, Sehrish Abbas, Shahid Iqbal, Kashif Jilani, Sobhy M. Ibrahim, Shagufta Kamal, Sadia Ata, Misbah Sultan, and Munawar Iqbal
- Subjects
Materials science ,General Chemical Engineering ,Doping ,Industrial chemistry ,Nanoparticle ,02 engineering and technology ,Dielectric ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Ferroelectricity ,0104 chemical sciences ,Catalysis ,Chemical engineering ,Photocatalysis ,Microemulsion ,0210 nano-technology - Abstract
In the present study, La1 − xCrxFeO3(x = 0.0, 0.3, 0.6, 0.9, 1.0) was synthesized by micro-emulsion route and characterized by X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR), Scanning electron microscope (SEM), Energy-dispersive X-ray (EDX) techniques. The dielectric, ferroelectric and photocatalytic properties were investigated and compared with un-doped material. The XRD analysis revealed orthorhombic geometry of La1 − xCrxFeO3(x = 0.0, 0.3, 0.6, 0.9, 1.0), Cr was doped successfully into the lattice structure of LaFeO3and particles were spherical and in agglomerated form. The grain sizes were recorded to be 15, 16.9, 17.1, 17.65 and 18.3 (nm) for La1 − xCrxFeO3(x = 0.0, 0.3, 0.6, 0.9, 1.0), respectively. EDX analysis confirmed the purity of LaCrFeO3samples. The lattice parameters, bulk density, X-ray density, crystalline size and porosity were determined were also determined of all the La1 − xCrxFeO3samples. The dielectric constant and dielectric loss values decreased at higher frequency and Cr concentration affected the dielectric properties. The photocatalytic activity (PCA) was evaluated by degrading Congo Red (CR) dye under solar light irradiation and up to 85.43% dye degradation was achieved within 45 min of irradiation. Phyto-toxicity analysis before and after dye degradation was performed, which revealed the toxicity reduction in response of dye degradation. Results revealed that lanthanum ferrite (perovskite) doping with Cr could possibly be employed to enhance the ferroelectric, dielectric and photocatalytic properties.
- Published
- 2020
- Full Text
- View/download PDF
41. Induction of Erythrocyte Shrinkage by Omeprazole
- Author
-
Kashif Jilani, Ismat Bibi, Abu Bakar Siddique, Muhammad Sikandar, Muhammad Qasim Akbar, Muhammad Riaz, Ayesha Naveed, Ghulam Rasool, Zahid Mushtaq, Muhammad Irfan, Asma Asghar, and Sajida Ilyas
- Subjects
0301 basic medicine ,medicine.drug_class ,Health, Toxicology and Mutagenesis ,Proton-pump inhibitor ,chemistry.chemical_element ,Pharmacology ,Calcium ,Toxicology ,medicine.disease_cause ,Superoxide dismutase ,03 medical and health sciences ,0302 clinical medicine ,eryptosis ,medicine ,oxidative stress ,Omeprazole ,chemistry.chemical_classification ,Chemical Health and Safety ,calcium ,biology ,Chemistry ,Glutathione peroxidase ,lcsh:RM1-950 ,Public Health, Environmental and Occupational Health ,medicine.disease ,Hemolysis ,cell size ,030104 developmental biology ,lcsh:Therapeutics. Pharmacology ,Catalase ,030220 oncology & carcinogenesis ,biology.protein ,Original Article ,Oxidative stress ,medicine.drug - Abstract
Omeprazole, a proton pump inhibitor blocks the H+/K+-ATPase channels of gastric parietal cells. It is used for the treatment of peptic ulcer. Prolonged use of omeprazole may involve in inducing anemia. The key marker of eryptosis includes membrane blebbing, cell shrinkage and phosphatidylserine (PS) exposure at the cell surface. In current study, the eryptotic, oxidative as well as hemolytic effects of therapeutical doses (0.5, 1 and 1.5 µM) of omeprazole were investigated after exposing erythrocytes for 48 hours. Investigation of eryptosis was done by cell size measurement, PS exposure determination and calcium channel inhibition. As a possible mechanism of omeprazole induced eryptosis, oxidative stress was investigated by determining the catalase, glutathione peroxidase and superoxide dismutase activities. Similarly, necrotic effect of omeprazole on erythrocytes was also evaluated through hemolysis measurement. Results of our study illustrated that 1.5 µM of omeprazole may induce significant decrease in superoxide dismutase, glutathione peroxidase and catalase activities as well as triggered the erythrocytes shrinkage, PS exposure and hemolysis. Role of calcium was also confirmed in inducing erythrocyte shrinkage. It is concluded that the exposure of erythrocytes with 1.5 µM omeprazole may enhance the rate of eryptosis and hemolysis by inducing oxidative stress.
- Published
- 2020
42. Stimulation of Erythrocyte Membrane Blebbing by Naproxen Sodium
- Author
-
Saba Siddiq, Sajida Ilyas, Muhammad Riaz, Ayesha Naveed, Ismat Bibi, Kashif Jilani, Muhammad Irfan, Haq Nawaz, Asma Asghar, and Muhammad Sikandar
- Subjects
0301 basic medicine ,Programmed cell death ,Health, Toxicology and Mutagenesis ,Analgesic ,Naproxen Sodium ,Pharmacology ,Toxicology ,medicine.disease_cause ,Superoxide dismutase ,03 medical and health sciences ,0302 clinical medicine ,eryptosis ,medicine ,oxidative stress ,chemistry.chemical_classification ,Chemical Health and Safety ,calcium ,biology ,Chemistry ,Glutathione peroxidase ,lcsh:RM1-950 ,Public Health, Environmental and Occupational Health ,medicine.disease ,Hemolysis ,cell size ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,Catalase ,030220 oncology & carcinogenesis ,biology.protein ,Original Article ,Oxidative stress - Abstract
Naproxen sodium is a nonsteroidal anti-inflammatory drug (NSAID) having antipyretic and analgesic properties, mainly used for the treatment of rheumatoid arthritis and osteoarthritis. Eryptosis is an alternative term used for suicidal erythrocyte death. In the current study, eryptotic effect of naproxen sodium characterized by membrane blebbing was investigated in erythrocytes after 48 hours of treatment with different concentrations (1-25 µM). The experimental work related to investigation of eryptosis was done by cell size measurement and confirmation of calcium role in the induction of membrane blebbing. As a possible mechanism of eryptosis, oxidative stress induced by naproxen sodium was determined by catalase, glutathione peroxidase, and superoxide dismutase activities. Similarly, hemolytic effect of naproxen sodium was also determined by hemolysis measurement. Results of our study illustrated that the therapeutic doses (10-25 µM) of naproxen sodium induce oxidative stress, confirmed by significant decrease in superoxide dismutase, catalase, and glutathione peroxidase activities that lead to the triggering of cell death by eryptosis and hemolysis.
- Published
- 2020
43. Cu nanoparticles synthesis using biological molecule of P. granatum seeds extract as reducing and capping agent: Growth mechanism and photo-catalytic activity
- Author
-
Sadia Ata, Ismat Bibi, Shazia Nouren, Munawar Iqbal, Nosheen Nazar, Muhammad Umair, Kashif Jilani, and Shagufta Kamal
- Subjects
Scanning electron microscope ,Metal ions in aqueous solution ,Analytical chemistry ,Metal Nanoparticles ,Nanoparticle ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,chemistry.chemical_compound ,Structural Biology ,Fourier transform infrared spectroscopy ,Molecular Biology ,Plant Extracts ,Green Chemistry Technology ,General Medicine ,Photochemical Processes ,021001 nanoscience & nanotechnology ,Copper ,0104 chemical sciences ,chemistry ,Seeds ,Photocatalysis ,Particle size ,0210 nano-technology ,Methylene blue ,Nuclear chemistry - Abstract
In view of extended applications of nanoparticles, the nanoparticles synthesis is an extensive research field and green synthesis is one of the co-friendly methodologies. Plant extract mediated synthesis of nanoparticles has gained much attention in current decade. In current investigation, copper nanoparticles (CuNPs) were prepared using P. granatum seeds extract (biological molecules) from copper(II) chloride salt. The synthesized CuNPs were characterized by UV-vis spectroscopy, X-ray diffraction measurements (XRD), scanning electron microscopy (SEM), Energy Dispersive X- Ray Spectroscopy (EDX), Fourier transform infra-red spectroscopy (FTIR) and atomic force microscopy techniques. The CuNPs formation occurred through reduction of metal ions followed by nucleation. The size of the CuNPs was in the range of 40-80nm (average particle size was 43.9nm) with semi spherical shape and uniformly distribution. Photocatalytic activity was evaluated by degrading methylene blue dye (150mg/L) at various CuNPs doses (10mg/L-100mg/L). The synthesized CuNPs showed excellent PCA for the degradation of methylene blue (MB) under solar light irradiation and up to 87.11% degradation was achieved. The oxidative degradation mechanism for MB was proposed. In view of efficient PCA, the use of biological molecules of P. granatum seeds extracts for the synthesis of CuNPs.
- Published
- 2018
- Full Text
- View/download PDF
44. Nickel nanoparticle synthesis using Camellia Sinensis as reducing and capping agent: Growth mechanism and photo-catalytic activity evaluation
- Author
-
Farzana Majid, Sadia Ata, Kashif Jilani, Munawar Iqbal, Nosheen Nazar, Ismat Bibi, Shazia Nouren, Shagufta Kamal, Ansar Abbas, Muhammad Amir, and Adeel Ahmed
- Subjects
Materials science ,Scanning electron microscope ,Metal Nanoparticles ,chemistry.chemical_element ,Nanoparticle ,Nanotechnology ,Chemistry Techniques, Synthetic ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Camellia sinensis ,Catalysis ,chemistry.chemical_compound ,Nickel ,Structural Biology ,Crystal violet ,Molecular Biology ,Plant Extracts ,General Medicine ,Photochemical Processes ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Plant Leaves ,chemistry ,X-ray crystallography ,Photocatalysis ,Degradation (geology) ,0210 nano-technology ,Oxidation-Reduction ,Nuclear chemistry - Abstract
Recently, the biosynthesis of nanoparticle attracted the attention of scientific community due to its simplicity, ease and eco-friendly nature. In the present study, Camellia Sinensis (C. Sinensis) leaves extract was employed for the synthesis of nickel nanoparticles (NiNPs). The fabricated NiNPs were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) and X-ray diffraction techniques. The photocatalytic activity (PCA) was evaluated by degrading crystal violet (CV) dye. The NiNPs size was in the range of 43.87-48.76nm, spherical in shape and uniformly distributed with magnetization saturation of 0.073 emu/g. The NiNPs showed promising PCA under solar light irradiation. At optimized conditions, up to 99.5% CV dye degradation was achieved. Results revealed that biosynthesis can be adopted for the synthesis of NiNPs in nano-size range since it is simple, cost effective and eco-friendly in nature versus physico-chemical methods.
- Published
- 2017
- Full Text
- View/download PDF
45. Green and eco-friendly synthesis of cobalt-oxide nanoparticle: Characterization and photo-catalytic activity
- Author
-
Haq Nawaz, Shazia Nouren, Ismat Bibi, Nosheen Nazar, Misbah Sultan, Munawar Iqbal, Sadia Ata, Yursa Safa, Fariha Rehman, Kashif Jilani, Shagufta Kamal, and Mazhar Abbas
- Subjects
inorganic chemicals ,Materials science ,Nanoparticle Characterization ,Scanning electron microscope ,General Chemical Engineering ,technology, industry, and agriculture ,chemistry.chemical_element ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,chemistry ,Mechanics of Materials ,Degradation (geology) ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Cobalt ,Cobalt oxide ,Powder diffraction ,Nuclear chemistry - Abstract
Cobalt-oxide nanoparticles (NPs) were fabricated using Punica granatum peel extract from cobalt nitrate hexahydrate at low temperature. The synthesized cobalt-oxide NPs were characterized using X-ray powder diffraction, scanning electron microscopy, energy-dispersive X-ray, atomic force microscopy, fourier transform infrared spectroscopy and UV-visible techniques. The cobalt-oxide NPs were in highly uniform shape and size was in the size of 40–80 nm. Photo-catalytic activity (PCA) of the synthesized NPs was evaluated by degrading Remazol Brilliant Orange 3R (RBO 3R) dye and a degradation of 78.45% was achieved (dye conc. 150 mg/L) using 0.5 g cobalt-oxide NPs for 50 min irradiation time. In view of eco-benign and cost-effective nature, the present investigation revealed that P. granatum could be used for the synthesis of cobalt-oxide NPs for photo-catalytic applications.
- Published
- 2017
- Full Text
- View/download PDF
46. Raman spectroscopy of blood plasma samples from breast cancer patients at different stages
- Author
-
H.F. Nargis, Muhammad Muddassar, Haq Nawaz, Haq Nawaz Bhatti, Muhammad Irfan Majeed, Tahir Mahmood, Franck Bonnier, Nosheen Rashid, Hugh J. Byrne, Kashif Jilani, M. Saleem, Allah Ditta, Department of Chemistry, University of Agriculture, Faisalabad, Pakistan, University of Agriculture Faisalabad - UAF (PAKISTAN), University of Central Punjab, Faisalabad, Department of Biosciences, COMSATS University Islamabad, COMSATS University Islamabad, National Institute of Lasers and Optronics (NILOP), Department of Biochemistry, University of Agriculture, Faisalabad, Nanomédicaments et Nanosondes, EA 6295 (NMNS), Université de Tours, Focas Research Institute [Dublin], Technological University [Dublin] (TU), and Université de Tours (UT)
- Subjects
Pathology ,medicine.medical_specialty ,multivariate data analysis ,Pharmacology, Toxicology and Environmental Health ,Breast Neoplasms ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,02 engineering and technology ,Spectrum Analysis, Raman ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,symbols.namesake ,Breast cancer ,breast cancer ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Blood plasma ,Biomarkers, Tumor ,medicine ,Humans ,Breast ,Stage (cooking) ,Spectral data ,Instrumentation ,Spectroscopy ,Cancer staging ,Principal Component Analysis ,Plasma samples ,Chemistry ,cancer staging ,Discriminant Analysis ,021001 nanoscience & nanotechnology ,medicine.disease ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,3. Good health ,Raman spectroscopy ,symbols ,Female ,0210 nano-technology ,blood plasma - Abstract
International audience; Raman spectroscopy was employed for the characterization of blood plasma samples from patients at different stages of breast cancer. Blood plasma samples taken from clinically diagnosed breast cancer patients were compared with healthy controls using multivariate data analysis techniques (principal components analysis – PCA) to establish Raman spectral features which can be considered spectral markers of breast cancer development. All the stages of the disease can be differentiated from normal samples. It is also found that stage 2 and 3 are biochemically similar, but can be differentiated from each other by PCA. The Raman spectral data of the stage 4 is found to be biochemically distinct, but very variable between patients. Raman spectral features associated with DNA and proteins were identified, which are exclusive to patient plasma samples. Moreover, there are several other spectral features which are strikingly different in the blood plasma samples of different stages of breast cancer. In order to further explore the potential of Raman spectroscopy as the basis of a minimally invasive screening technique for breast cancer diagnosis and staging, PCA-Factorial Discriminant Analysis (FDA) was employed to classify the Raman spectral datasets of the blood plasma samples of the breast cancer patients, according to different stages of the disease, yielding promisingly high values of sensitivity and specificity for all stages.
- Published
- 2019
- Full Text
- View/download PDF
47. Atorvastatin Induced Erythrocytes Membrane Blebbing
- Author
-
Asma Asghar, Ismat Bibi, Mazhar Hussain Ranjha, Muhammad Riaz, Muhammad Irfan, Rumaisa Bashir Rana, Kashif Jilani, and Muhammad Shahid
- Subjects
0301 basic medicine ,Health, Toxicology and Mutagenesis ,Atorvastatin ,chemistry.chemical_element ,Pharmacology ,Calcium ,Reductase ,Toxicology ,medicine.disease_cause ,Superoxide dismutase ,03 medical and health sciences ,0302 clinical medicine ,eryptosis ,medicine ,oxidative stress ,chemistry.chemical_classification ,Chemical Health and Safety ,calcium ,biology ,Chemistry ,Glutathione peroxidase ,lcsh:RM1-950 ,Public Health, Environmental and Occupational Health ,nutritional and metabolic diseases ,Calcium Channel Inhibition ,cell size ,030104 developmental biology ,lcsh:Therapeutics. Pharmacology ,Catalase ,030220 oncology & carcinogenesis ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Original Article ,Oxidative stress ,medicine.drug - Abstract
Atorvastatin, an inhibitor of 3-hydroxy-3-methylglutaryl-coenzymeA reductase, is usually used for the treatment of hypercholesterolemia. Besides its pharmacological and side actions, its toxic effects on human nucleus devoid of erythrocytes are still unknown. Eryptosis is an alternative term used for suicidal erythrocyte death. Membrane blebbing is among the common markers of eryptosis. In this study, eryptotic effect of atorvastatin was investigated by exposing the erythrocytes for 48 hours to different concentrations (1-10 µM) of atorvastatin. The experimental work related to investigation of eryptosis was done by cell size measurement and calcium channel inhibition. As a possible mechanism of eryptosis, atorvastatin-induced oxidative stress was evaluated by determining catalase, glutathione peroxidase, and superoxide dismutase activities. Similarly, necrotic effect of atorvastatin was also determined by hemolytic assay. Results of our study illustrated that the tested doses of atorvastatin may induce oxidative stress as observed by significant reduction in superoxide dismutase, glutathione peroxidase, and catalase activities as well as induce eryptosis, featured by erythrocytes membrane blebbing. The study concluded that induction of oxidative stress by atorvastatin may lead to eryptosis.
- Published
- 2019
48. Comparison of surface enhanced Raman spectroscopy and Raman spectroscopy for the detection of breast cancer based on serum samples
- Author
-
H.F. Nargis, Haq Nawaz, Haq Nawaz Bhatti, Kashif Jilani, and M. Saleem
- Subjects
Breast Neoplasms ,02 engineering and technology ,Spectrum Analysis, Raman ,010402 general chemistry ,Sensitivity and Specificity ,01 natural sciences ,Analytical Chemistry ,symbols.namesake ,Nuclear magnetic resonance ,Breast cancer ,Blood serum ,medicine ,Humans ,Spectral data ,Instrumentation ,Spectroscopy ,Principal Component Analysis ,Receiver operating characteristic ,Chemistry ,Discriminant Analysis ,Surface-enhanced Raman spectroscopy ,021001 nanoscience & nanotechnology ,Serum samples ,medicine.disease ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Healthy individuals ,symbols ,0210 nano-technology ,Raman spectroscopy - Abstract
In this study, surface enhanced Raman spectroscopy (SERS) and Raman spectroscopy (RS), are employed for the classification of different stages of breast cancer using clinically diagnosed serum samples from breast cancer patients and healthy individuals. These serum samples are compared for their spectral features acquired by SERS and RS to establish spectral features that can be considered as spectral markers of breast cancer diagnosis and classification. SERS features related to DNA, proteins and lipids were observed which are solely observed in the serum samples of patients at different stages of breast cancer as compared to healthy samples. In order to explore the capability of SERS and RS and their comparison as an analytical tool for the efficient understanding of the progression of breast cancer, Principal Component Analysis (PCA) is done for the SERS and RS spectra of control, stage 2, stage 3 and stage 4. Furthermore, the Partial Least Squares-Discriminant Analysis (PLS-DA) was performed to compare the diagnostic performance of SERS and Raman spectroscopy for the classification of disease positive samples and healthy ones. The sensitivity and specificity and area under receiver operating characteristic (AUROC) curve values for SERS data were 90%, 98.4%, and 94% respectively which were higher as compared to Raman spectral data for which these values were found to be 88.2%, 97.7%, and 83.4% respectively.
- Published
- 2021
- Full Text
- View/download PDF
49. Therapeutic potential of manipulating suicidal erythrocyte death
- Author
-
Florian Lang, Kashif Jilani, and Elisabeth Lang
- Subjects
Erythrocytes ,Anemia ,Thalassemia ,Clinical Biochemistry ,Apoptosis ,Biology ,medicine.disease_cause ,Sepsis ,Drug Discovery ,medicine ,Animals ,Humans ,Pharmacology ,Phagocytes ,Calpain ,Iron deficiency ,medicine.disease ,Sickle cell anemia ,Uremia ,Malaria ,Oxidative Stress ,Drug Design ,Immunology ,biology.protein ,Molecular Medicine ,Oxidative stress - Abstract
Eryptosis, the suicidal erythrocyte death, is characterized by erythrocyte shrinkage and phosphatidylserine translocation to the erythrocyte surface. Eryptosis is triggered by cell stress such as energy depletion and oxidative stress, by Ca(2+)-entry, ceramide, caspases, calpain and/or altered activity of several kinases. Phosphatidylserine-exposing erythrocytes adhere to the vascular wall and may thus impede microcirculation. Eryptotic cells are further engulfed by phagocytes and thus rapidly cleared from circulation.Stimulation of eryptosis contributes to anemia of several clinical conditions such as metabolic syndrome, diabetes, malignancy, hepatic failure, heart failure, uremia, hemolytic uremic syndrome, sepsis, fever, dehydration, mycoplasma infection, malaria, iron deficiency, sickle cell anemia, thalassemia, glucose-6-phosphate dehydrogenase deficiency and Wilson's disease. On the other hand, eryptosis with subsequent clearance of infected erythrocytes in malaria may counteract parasitemia.In theory, anemia due to excessive eryptosis could be alleviated by treatment with small molecules inhibiting eryptosis. In malaria, stimulators of eryptosis may accelerate death of infected erythrocytes and thus favorably influence the clinical course of the disease. Many small molecules inhibit or stimulate eryptosis. Several stimulators favorably influence murine malaria. Further preclinical and subsequent clinical studies are required to elucidate the therapeutic potential of stimulators or inhibitors of eryptosis.
- Published
- 2015
- Full Text
- View/download PDF
50. Vitamin D-Rich Diet in Mice Modulates Erythrocyte Survival
- Author
-
Kashif Jilani, Christine Zelenak, Florian Lang, Rexhep Rexhepaj, Adrian Lupescu, Rosi Bissinger, Syed M. Qadri, and Elisabeth Lang
- Subjects
Vitamin ,lcsh:Diseases of the circulatory (Cardiovascular) system ,medicine.medical_specialty ,Programmed cell death ,Anemia ,Eryptosis ,Energy depletion ,Hyperosmotic shock ,Phosphatidylserines ,Biology ,lcsh:RC870-923 ,vitamin D deficiency ,Mice ,chemistry.chemical_compound ,Osmotic Pressure ,Internal medicine ,Cell shrinkage ,lcsh:Dermatology ,medicine ,Vitamin D and neurology ,Animals ,Vitamin D ,Erythropoietin ,Cell Size ,Erythrocyte Membrane ,Erythrocyte Aging ,Vitamins ,General Medicine ,Phosphatidylserine ,lcsh:RL1-803 ,lcsh:Diseases of the genitourinary system. Urology ,medicine.disease ,Blood Cell Count ,Diet ,Mice, Inbred C57BL ,Endocrinology ,Biochemistry ,chemistry ,lcsh:RC666-701 ,Nephrology ,Apoptosis ,Calcium ,Female ,Cardiology and Cardiovascular Medicine ,medicine.drug - Abstract
Background/Aims: Epidemiological evidence suggests that vitamin D deficiency is associated with anemia. The potent metabolite 1,25(OH)2 vitamin D3 [1,25(OH)2D3] activates various signaling cascades regulating a myriad of cellular functions including suicidal cell death or apoptosis. Suicidal death of erythrocytes or eryptosis is characterized by cell shrinkage and cell membrane scrambling leading to phosphatidylserine (PS) externalization. Stimulation of eryptosis may limit lifespan of circulating erythrocytes and thus cause anemia. In the present study, we explored the effect of a high vitamin D diet (10,000 I.U. vitamin D for 14 days) in mice on eryptosis. Methods: Plasma concentrations of erythropoietin were estimated using an immunoassay kit, blood count using an electronic hematology particle counter, relative reticulocyte numbers using Retic-COUNT® reagent, PS exposure at the cell surface from annexin V binding, cell volume from forward scatter, and cytosolic Ca2+ ([Ca2+]i) from Fluo3-fluorescence in FACS analysis. Results: Vitamin D treatment decreased mean corpuscular volume, reticulocyte count, and plasma erythropoietin levels. Vitamin D treatment slightly but significantly decreased forward scatter but did not significantly modify spontaneous PS exposure and [Ca2+]i of freshly drawn erythrocytes. Vitamin D treatment augmented the stimulation of PS exposure and cell shrinkage following exposure to hyperosmotic shock (addition of 550 mM sucrose) or energy depletion (glucose removal) without significantly modifying [Ca2+]i. Conclusions: The present observations point to a subtle effect of exogenous vitamin D supplementation on erythrocyte survival.
- Published
- 2015
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.