1. Assessment of dd-cfDNA Levels in Clinically Stable Lung Allograft Recipients Beyond the Initial 2 y Posttransplant
- Author
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Anil J. Trindade, MD, Kaitlyn C. Chapin, AGNP, Amy Mullican, RN, Jennifer N. Gray, PharmD, Haley Hoy, AGNP, PhD, Caitlin T. Demarest, MD, PhD, Eric S. Lambright, MD, Katie A. McPherson, MD, Stephanie G. Norfolk, MD, Ivan M. Robbins, MD, Matthew Bacchetta, MD, MBA, David B. Erasmus, MD, and Ciara M. Shaver, MD, PhD
- Subjects
Surgery ,RD1-811 - Abstract
Background. Donor-derived cell-free DNA (dd-cfDNA) is a useful biomarker for the diagnosis of acute allograft injury within the first 1 to 2 y after lung transplant, but its utility for diagnosing chronic lung allograft dysfunction (CLAD) has not yet been studied. Understanding baseline dd-cfDNA kinetics beyond the initial 2 y posttransplant is a necessary first step in determining the utility of dd-cfDNA as a CLAD biomarker. We seek to establish baseline dd-cfDNA% levels in clinically stable lung allograft recipients who are >2 y posttransplant. Methods. We performed a prospective, single-center, observational study to identify plasma dd-cfDNA levels in clinically stable lung allograft recipients >2 y posttransplant. Results. Fifty-one subjects were enrolled and ≥3 baseline dd-cfDNA measurements were acquired during a median of 252 d. The median baseline percent dd-cfDNA level in our cohort was 0.45% (interquartile range [IQR], 0.26–0.69). There were statistically significant differences in dd-cfDNA based on posttransplant duration (≤5 y posttransplant median 0.41% [IQR, 0.21–0.64] versus >5 y posttransplant median 0.50% [IQR, 0.33–0.76]; P 2 y post–lung transplant. These findings lay the groundwork for the study of dd-cfDNA as a possible biomarker for CLAD.
- Published
- 2022
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