25 results on '"Katowa, Ben"'
Search Results
2. Therapeutic Efficacy of Artemether-Lumefantrine for Uncomplicated Falciparum Malaria in Northern Zambia.
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Ippolito, Matthew M, Pringle, Julia C, Siame, Mwiche, Katowa, Ben, Aydemir, Ozkan, Oluoch, Peter O, Huang, Liusheng, Aweeka, Francesca T, Bailey, Jeffrey A, Juliano, Jonathan J, Meshnick, Steven R, Shapiro, Theresa A, Moss, William J, and Thuma, Philip E
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Medical Microbiology ,Biomedical and Clinical Sciences ,Clinical Sciences ,Genetics ,Clinical Research ,Orphan Drug ,Vector-Borne Diseases ,Malaria ,Antimicrobial Resistance ,Infectious Diseases ,Rare Diseases ,Pediatric ,Development of treatments and therapeutic interventions ,5.1 Pharmaceuticals ,Evaluation of treatments and therapeutic interventions ,6.1 Pharmaceuticals ,Infection ,Good Health and Well Being ,Antimalarials ,Artemether ,Lumefantrine Drug Combination ,Child ,Preschool ,Drug Resistance ,Female ,Humans ,Infant ,Malaria ,Falciparum ,Male ,Parasitemia ,Plasmodium falciparum ,Zambia ,Medical and Health Sciences ,Tropical Medicine ,Biomedical and clinical sciences ,Health sciences - Abstract
Artemether-lumefantrine (AL) is a first-line agent for uncomplicated malaria caused by Plasmodium falciparum. The WHO recommends periodic therapeutic efficacy studies of antimalarial drugs for the detection of malaria parasite drug resistance and to inform national malaria treatment policies. We conducted a therapeutic efficacy study of AL in a high malaria transmission region of northern Zambia from December 2014 to July 2015. One hundred children of ages 6 to 59 months presenting to a rural health clinic with uncomplicated falciparum malaria were admitted for treatment with AL (standard 6-dose regimen) and followed weekly for 5 weeks. Parasite counts were taken every 6 hours during treatment to assess parasite clearance. Recurrent episodes during follow-up (n = 14) were genotyped to distinguish recrudescence from reinfection and to identify drug resistance single nucleotide polymorphisms (SNPs) and multidrug resistance protein 1 (mdr1) copy number variation. Day 7 lumefantrine concentrations were measured for correspondence with posttreatment reinfection. All children who completed the parasite clearance portion of the study (n = 94) were microscopy-negative by 72 hours. The median parasite elimination half-life was 2.7 hours (interquartile range: 2.1-3.3). Genotype-corrected therapeutic efficacy was 98.8% (95% CI: 97.6-100). Purported artemisinin and lumefantrine drug resistance SNPs in atp6, 3D7_1451200, and mdr1 were detected but did not correlate with parasite recurrence, nor did day 7 lumefantrine concentrations. In summary, AL was highly effective for the treatment of uncomplicated falciparum malaria in northern Zambia during the study period. The high incidence of recurrent parasitemia was consistent with reinfection due to high, perennial malaria transmission.
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- 2020
3. Genetic Evidence of Focal Plasmodium falciparum Transmission in a Pre-elimination Setting in Southern Province, Zambia.
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Pringle, Julia, Tessema, Sofonias, Wesolowski, Amy, Chen, Anna, Murphy, Maxwell, Carpi, Giovanna, Shields, Timothy, Hamapumbu, Harry, Searle, Kelly, Kobayashi, Tamaki, Katowa, Ben, Musonda, Michael, Stevenson, Jennifer, Thuma, Philip, Greenhouse, Bryan, Moss, William, and Norris, Douglas
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case detection ,malaria ,microsatellites ,molecular epidemiology ,reactive ,screen-and-treat ,Disease Eradication ,Genotyping Techniques ,Humans ,Malaria ,Falciparum ,Microsatellite Repeats ,Plasmodium falciparum ,Population Surveillance ,Spatio-Temporal Analysis ,Zambia - Abstract
BACKGROUND: Southern Province, Zambia has experienced a dramatic decline in Plasmodium falciparum malaria transmission in the past decade and is targeted for elimination. Zambias National Malaria Elimination Program recommends reactive case detection (RCD) within 140 m of index households to enhance surveillance and eliminate remaining transmission foci. METHODS: To evaluate whether RCD captures local transmission, we genotyped 26 microsatellites from 106 samples collected from index (n = 27) and secondary (n = 79) cases detected through RCD in the Macha Hospital catchment area between January 2015 and April 2016. RESULTS: Participants from the same RCD event harbored more genetically related parasites than those from different RCD events, suggesting that RCD captures, at least in part, infections related through local transmission. Related parasites clustered in space and time, up to at least 250 m from index households. Spatial analysis identified a putative focal transmission hotspot. CONCLUSIONS: The current RCD strategy detects focal transmission events, although programmatic guidelines to screen within 140 m of index households may fail to capture all secondary cases. This study highlights the utility of parasite genetic data in assessing programmatic interventions, and similar approaches may be useful to malaria elimination programs seeking to tailor intervention strategies to the underlying transmission epidemiology.
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- 2019
4. Ineffectiveness of international travel restrictions to contain spread of the SARS-CoV-2 Omicron BA.1 variant: a continent-wide laboratory-based observational study from Africa
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Fischer, Carlo, primary, Maponga, Tongai Gibson, additional, Yadouleton, Anges, additional, Abilio, Nuro, additional, Aboce, Emmanuel, additional, Adewumi, Praise, additional, Afonso, Pedro, additional, Akorli, Jewelna, additional, Andriamandimby, Soa Fy, additional, Anga, Latifa, additional, Ashong, Yvonne, additional, Beloufa, Mohamed Amine, additional, Bensalem, Aicha, additional, Birtles, Richard, additional, Magloire Boumba, Anicet Luc, additional, Bwanga, Freddie, additional, Chaponda, Mike, additional, Chibukira, Paradzai, additional, Chico, R Matthew, additional, Chileshe, Justin, additional, Choga, Wonderful, additional, Chongwe, Gershom, additional, Cisse, Assana, additional, Cisse, Fatoumata, additional, D Alessandro, Umberto, additional, de Lamballerie, Xavier, additional, de Morais, Joana F.M., additional, Derrar, Fawzi, additional, Dia, Ndongo, additional, Diarra, Youssouf, additional, Doumbia, Lassina, additional, Drosten, Christian, additional, Dussart, Philippe, additional, Echodu, Richard, additional, Luedde, Tom, additional, Eloualid, Abdelmajid, additional, Faye, Ousmane, additional, Feldt, Torsten, additional, Fruehauf, Anna, additional, Gaseitsiwe, Simani, additional, Halatoko, Afiwa, additional, Ilouga, Pauliana-Vanessa, additional, Ismael, Nalia, additional, Jambou, Ronan, additional, Jarju, Sheikh, additional, Kamprad, Antje, additional, Katowa, Ben, additional, Kayiwa, John, additional, Kingwara, Leonard, additional, Koita, Ousmane, additional, Lacoste, Vincent, additional, Lagare, Adamou, additional, Landt, Olfert, additional, Lekana-Douki, Sonia Etenna, additional, Lekana-Douki, Jean-Bernard, additional, Iipumbu, Etuhole, additional, Loemba, Hugues, additional, Lutwama, Julius, additional, Mamadou, Santou, additional, Maman, Issaka, additional, Manyisa, Brendon, additional, Martinez, Pedro A., additional, Matoba, Japhet, additional, Mhuulu, Lusia, additional, Moreira-Soto, Andres, additional, Moyo, Sikhulile, additional, Mwangi, Judy, additional, Ndilimabaka, Nadine, additional, Nassuna, Charity Angella, additional, Ndiath, Mamadou Ousmane, additional, Nepolo, Emmanuel, additional, Njouom, Richard, additional, Nourlil, Jalal, additional, Nyanjom, Steven Ger, additional, Odari, Eddy Okoth, additional, Okeng, Alfred, additional, Ouoba, Jean Bienvenue, additional, Owusu, Michael, additional, Donkor, Irene Owusu, additional, Phadu, Karabo Kristen, additional, Phillips, Richard Odame, additional, Preiser, Wolfgang, additional, Roques, Pierre, additional, Ruhanya, Vurayai, additional, Salah, Fortune, additional, Salifou, Sourakatou, additional, Sall, Amadou Alpha, additional, Sylverken, Augustina Angelina, additional, Tagnouokam-Ngoupo, Paul Alain, additional, Tarnagda, Zekiba, additional, Tchikaya, Francis Olivier, additional, Tordo, Noel, additional, Tufa, Tafese Beyene, additional, and Drexler, Jan Felix, additional
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- 2024
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5. Declining Age-Specific Seroprevalence and Seroconversion Rates in Plasmodium falciparum from 2009 to 2018 Documents Progress toward Malaria Elimination in Southern Zambia
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Katowa, Ben, primary, Hamapumbu, Harry, additional, Thuma, Philip E., additional, Bérubé, Sophie, additional, Wesolowski, Amy, additional, Moss, William J., additional, Kobayashi, Tamaki, additional, and _, _, additional
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- 2023
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6. Retraction
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Fischer, Carlo, primary, Maponga, Tongai Gibson, additional, Yadouleton, Anges, additional, Abílio, Nuro, additional, Aboce, Emmanuel, additional, Adewumi, Praise, additional, Afonso, Pedro, additional, Akorli, Jewelna, additional, Andriamandimby, Soa Fy, additional, Anga, Latifa, additional, Ashong, Yvonne, additional, Beloufa, Mohamed Amine, additional, Bensalem, Aicha, additional, Birtles, Richard, additional, Boumba, Anicet Luc Magloire, additional, Bwanga, Freddie, additional, Chaponda, Mike, additional, Chibukira, Paradzai, additional, Chico, R. Matthew, additional, Chileshe, Justin, additional, Chongwe, Gershom, additional, Cissé, Assana, additional, D’Alessandro, Umberto, additional, de Lamballerie, Xavier Nicolas, additional, de Morais, Joana F. M., additional, Derrar, Fawzi, additional, Dia, Ndongo, additional, Diarra, Youssouf, additional, Doumbia, Lassina, additional, Drosten, Christian, additional, Dussart, Philippe, additional, Echodu, Richard, additional, Eggers, Yannik, additional, Eloualid, Abdelmajid, additional, Faye, Ousmane, additional, Feldt, Torsten, additional, Frühauf, Anna, additional, Halatoko, Afiwa, additional, Ilouga, Pauliana-Vanessa, additional, Ismael, Nalia, additional, Jambou, Ronan, additional, Jarju, Sheikh, additional, Kamprad, Antje, additional, Katowa, Ben, additional, Kayiwa, John, additional, King’wara, Leonard, additional, Koita, Ousmane, additional, Lacoste, Vincent, additional, Lagare, Adamou, additional, Landt, Olfert, additional, Lekana-Douki, Sonia Etenna, additional, Lekana-Douki, Jean-Bernard, additional, Iipumbu, Etuhole, additional, Loemba, Hugues, additional, Lutwama, Julius, additional, Mamadou, Santou, additional, Maman, Issaka, additional, Manyisa, Brendon, additional, Martinez, Pedro A., additional, Matoba, Japhet, additional, Mhuulu, Lusia, additional, Moreira-Soto, Andres, additional, Mwangi, Judy, additional, N’dilimabaka, Nadine, additional, Nassuna, Charity Angella, additional, Ndiath, Mamadou Ousmane, additional, Nepolo, Emmanuel, additional, Njouom, Richard, additional, Nourlil, Jalal, additional, Nyanjom, Steven Ger, additional, Odari, Eddy Okoth, additional, Okeng, Alfred, additional, Ouoba, Jean Bienvenue, additional, Owusu, Michael, additional, Donkor, Irene Owusu, additional, Phadu, Karabo Kristen, additional, Phillips, Richard Odame, additional, Preiser, Wolfgang, additional, Ruhanya, Vurayai, additional, Salah, Fortune, additional, Salifou, Sourakatou, additional, Sall, Amadou Alpha, additional, Sylverken, Augustina Angelina, additional, Tagnouokam-Ngoupo, Paul Alain, additional, Tarnagda, Zekiba, additional, Tchikaya, Francis Olivier, additional, Tufa, Tafese Beyene, additional, and Drexler, Jan Felix, additional
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- 2022
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7. Gradual emergence followed by exponential spread of the SARS-CoV-2 Omicron variant in Africa
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Fischer, Carlo, primary, Maponga, Tongai Gibson, additional, Yadouleton, Anges, additional, Abílio, Nuro, additional, Aboce, Emmanuel, additional, Adewumi, Praise, additional, Afonso, Pedro, additional, Akorli, Jewelna, additional, Andriamandimby, Soa Fy, additional, Anga, Latifa, additional, Ashong, Yvonne, additional, Beloufa, Mohamed Amine, additional, Bensalem, Aicha, additional, Birtles, Richard, additional, Boumba, Anicet Luc Magloire, additional, Bwanga, Freddie, additional, Chaponda, Mike, additional, Chibukira, Paradzai, additional, Chico, R. Matthew, additional, Chileshe, Justin, additional, Chongwe, Gershom, additional, Cissé, Assana, additional, D’Alessandro, Umberto, additional, de Lamballerie, Xavier Nicolas, additional, de Morais, Joana F. M., additional, Derrar, Fawzi, additional, Dia, Ndongo, additional, Diarra, Youssouf, additional, Doumbia, Lassina, additional, Drosten, Christian, additional, Dussart, Philippe, additional, Echodu, Richard, additional, Eggers, Yannik, additional, Eloualid, Abdelmajid, additional, Faye, Ousmane, additional, Feldt, Torsten, additional, Frühauf, Anna, additional, Halatoko, Afiwa, additional, Ilouga, Pauliana-Vanessa, additional, Ismael, Nalia, additional, Jambou, Ronan, additional, Jarju, Sheikh, additional, Kamprad, Antje, additional, Katowa, Ben, additional, Kayiwa, John, additional, King’wara, Leonard, additional, Koita, Ousmane, additional, Lacoste, Vincent, additional, Lagare, Adamou, additional, Landt, Olfert, additional, Lekana-Douki, Sonia Etenna, additional, Lekana-Douki, Jean-Bernard, additional, Iipumbu, Etuhole, additional, Loemba, Hugues, additional, Lutwama, Julius, additional, Mamadou, Santou, additional, Maman, Issaka, additional, Manyisa, Brendon, additional, Martinez, Pedro A., additional, Matoba, Japhet, additional, Mhuulu, Lusia, additional, Moreira-Soto, Andres, additional, Mwangi, Judy, additional, N´dilimabaka, Nadine, additional, Nassuna, Charity Angella, additional, Ndiath, Mamadou Ousmane, additional, Nepolo, Emmanuel, additional, Njouom, Richard, additional, Nourlil, Jalal, additional, Nyanjom, Steven Ger, additional, Odari, Eddy Okoth, additional, Okeng, Alfred, additional, Ouoba, Jean Bienvenue, additional, Owusu, Michael, additional, Owusu Donkor, Irene, additional, Phadu, Karabo Kristen, additional, Phillips, Richard Odame, additional, Preiser, Wolfgang, additional, Ruhanya, Vurayai, additional, Salah, Fortune, additional, Salifou, Sourakatou, additional, Sall, Amadou Alpha, additional, Sylverken, Augustina Angelina, additional, Tagnouokam-Ngoupo, Paul Alain, additional, Tarnagda, Zekiba, additional, Tchikaya, Francis Olivier, additional, Tufa, Tafese Beyene, additional, and Drexler, Jan Felix, additional
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- 2022
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8. What is the prevalence of COVID-19 detection by PCR among deceased individuals in Lusaka, Zambia? A postmortem surveillance study
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Gill, Christopher J, primary, Mwananyanda, Lawrence, additional, MacLeod, William B, additional, Kwenda, Geoffrey, additional, Pieciak, Rachel C, additional, Etter, Lauren, additional, Bridges, Daniel, additional, Chikoti, Chilufya, additional, Chirwa, Sarah, additional, Chimoga, Charles, additional, Forman, Leah, additional, Katowa, Ben, additional, Lapidot, Rotem, additional, Lungu, James, additional, Matoba, Japhet, additional, Mwinga, Gift, additional, Mubemba, Benjamin, additional, Mupila, Zachariah, additional, Muleya, Walter, additional, Mwenda, Mulenga, additional, Ngoma, Benard, additional, Nakazwe, Ruth, additional, Nzara, Diana, additional, Pawlak, Natalie, additional, Pemba, Lillian, additional, Saasa, Ngonda, additional, Simulundu, Edgar, additional, Yankonde, Baron, additional, and Thea, Donald M, additional
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- 2022
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9. Temporal and spatial analysis of Plasmodium falciparum genomics reveals patterns of parasite connectivity in a low-transmission district in Southern Province, Zambia.
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Fola, Abebe A., Moser, Kara A., Aydemir, Ozkan, Hennelly, Chris, Kobayashi, Tamaki, Shields, Timothy, Hamapumbu, Harry, Musonda, Michael, Katowa, Ben, Matoba, Japhet, Stevenson, Jennifer C., Norris, Douglas E., Thuma, Philip E., Wesolowski, Amy, Moss, William J., Bailey, Jeffrey A., and Juliano, Jonathan J.
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PLASMODIUM falciparum ,GENOMICS ,MOLECULAR probes ,PARASITES ,MEDICAL centers ,REOVIRUSES - Abstract
Background: Understanding temporal and spatial dynamics of malaria transmission will help to inform effective interventions and strategies in regions approaching elimination. Parasite genomics are increasingly used to monitor epidemiologic trends, including assessing residual transmission across seasons and importation of malaria into these regions. Methods: In a low and seasonal transmission setting of southern Zambia, a total of 441 Plasmodium falciparum samples collected from 8 neighbouring health centres between 2012 and 2018 were genotyped using molecular inversion probes (MIPs n = 1793) targeting a total of 1832 neutral and geographically informative SNPs distributed across the parasite genome. After filtering for quality and missingness, 302 samples and 1410 SNPs were retained and used for downstream population genomic analyses. Results: The analyses revealed most (67%, n = 202) infections harboured one clone (monogenomic) with some variation at local level suggesting low, but heterogenous malaria transmission. Relatedness identity-by-descent (IBD) analysis revealed variable distribution of IBD segments across the genome and 6% of pairs were highly-related (IBD ≥ 0.25). Some of the highly-related parasite populations persisted across multiple seasons, suggesting that persistence of malaria in this low-transmission region is fueled by parasites "seeding" across the dry season. For recent years, clusters of clonal parasites were identified that were dissimilar to the general parasite population, suggesting parasite populations were increasingly fragmented at small spatial scales due to intensified control efforts. Clustering analysis using PCA and t-SNE showed a lack of substantial parasite population structure. Conclusion: Leveraging both genomic and epidemiological data provided comprehensive picture of fluctuations in parasite populations in this pre-elimination setting of southern Zambia over 7 years. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Policy Implications of the Southern and Central Africa International Center of Excellence for Malaria Research: Ten Years of Malaria Control Impact Assessments in Hypo-, Meso-, and Holoendemic Transmission Zones in Zambia and Zimbabwe
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Wesolowski, Amy, primary, Ippolito, Matthew M., additional, Gebhardt, Mary E., additional, Ferriss, Ellen, additional, Schue, Jessica L., additional, Kobayashi, Tamaki, additional, Chaponda, Mike, additional, Kabuya, Jean-Bertin, additional, Muleba, Mbanga, additional, Mburu, Monicah, additional, Matoba, Japhet, additional, Musonda, Michael, additional, Katowa, Ben, additional, Lubinda, Mukuma, additional, Hamapumbu, Harry, additional, Simubali, Limonty, additional, Mudenda, Twig, additional, Shields, Timothy M., additional, Hackman, Andre, additional, Shiff, Clive, additional, Coetzee, Maureen, additional, Koekemoer, Lizette L., additional, Munyati, Shungu, additional, Gwanzura, Lovemore, additional, Mutambu, Susan, additional, Stevenson, Jennifer C., additional, Thuma, Philip E., additional, Norris, Douglas E., additional, Bailey, Jeffrey A., additional, Juliano, Jonathan J., additional, Chongwe, Gershom, additional, Mulenga, Modest, additional, Simulundu, Edgar, additional, Mharakurwa, Sungano, additional, Agre, Peter, additional, Moss, William J., additional, and _, _, additional
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- 2022
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11. Scientific Findings of the Southern and Central Africa International Center of Excellence for Malaria Research: Ten Years of Malaria Control Impact Assessments in Hypo-, Meso-, and Holoendemic Transmission Zones in Zambia and Zimbabwe
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Ippolito, Matthew M., primary, Gebhardt, Mary E., additional, Ferriss, Ellen, additional, Schue, Jessica L., additional, Kobayashi, Tamaki, additional, Chaponda, Mike, additional, Kabuya, Jean-Bertin, additional, Muleba, Mbanga, additional, Mburu, Monicah, additional, Matoba, Japhet, additional, Musonda, Michael, additional, Katowa, Ben, additional, Lubinda, Mukuma, additional, Hamapumbu, Harry, additional, Simubali, Limonty, additional, Mudenda, Twig, additional, Wesolowski, Amy, additional, Shields, Timothy M., additional, Hackman, Andre, additional, Shiff, Clive, additional, Coetzee, Maureen, additional, Koekemoer, Lizette L., additional, Munyati, Shungu, additional, Gwanzura, Lovemore, additional, Mutambu, Susan, additional, Stevenson, Jennifer C., additional, Thuma, Philip E., additional, Norris, Douglas E., additional, Bailey, Jeffrey A., additional, Juliano, Jonathan J., additional, Chongwe, Gershom, additional, Mulenga, Modest, additional, Simulundu, Edgar, additional, Mharakurwa, Sungano, additional, Agre, Peter C., additional, Moss, William J., additional, and _, _, additional
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- 2022
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12. Genomic Surveillance of SARS-CoV-2 in the Southern Province of Zambia: Detection and Characterization of Alpha, Beta, Delta, and Omicron Variants of Concern
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Katowa, Ben, primary, Kalonda, Annie, additional, Mubemba, Benjamin, additional, Matoba, Japhet, additional, Shempela, Doreen Mainza, additional, Sikalima, Jay, additional, Kabungo, Boniface, additional, Changula, Katendi, additional, Chitanga, Simbarashe, additional, Kasonde, Mpanga, additional, Kapona, Otridah, additional, Kapata, Nathan, additional, Musonda, Kunda, additional, Monze, Mwaka, additional, Tembo, John, additional, Bates, Matthew, additional, Zumla, Alimuddin, additional, Sutcliffe, Catherine G., additional, Kajihara, Masahiro, additional, Yamagishi, Junya, additional, Takada, Ayato, additional, Sawa, Hirofumi, additional, Chilengi, Roma, additional, Mukonka, Victor, additional, Muleya, Walter, additional, and Simulundu, Edgar, additional
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- 2022
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13. Temporal and spatial analysis of Plasmodium falciparum genomics reveals patterns of connectivity in a low-transmission district in Southern Province, Zambia
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Moser, Kara A., primary, Aydemir, Ozkan, additional, Hennelly, Chris, additional, Kobayashi, Tamaki, additional, Shields, Timothy, additional, Hamapumbu, Harry, additional, Musonda, Michael, additional, Katowa, Ben, additional, Matoba, Japhet, additional, Stevenson, Jennifer C., additional, Norris, Douglas E., additional, Thuma, Philip E., additional, Wesolowski, Amy, additional, Moss, William J., additional, Bailey, Jeffrey A., additional, and Juliano, Jonathan J., additional
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- 2021
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14. Sustained Malaria Transmission despite Reactive Screen-and-Treat in a Low-Transmission Area of Southern Zambia
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Searle, Kelly M., primary, Katowa, Ben, additional, Musonda, Michael, additional, Pringle, Julia C., additional, Hamapumbu, Harry, additional, Matoba, Japhet, additional, Lubinda, Mukuma, additional, Shields, Timothy, additional, Kobayashi, Tamaki, additional, Stevenson, Jennifer C., additional, Norris, Douglas E., additional, Thuma, Philip E., additional, Wesolowski, Amy, additional, Moss, William J., additional, and _, _, additional
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- 2021
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15. Additional file 1 of Improving the efficiency of reactive case detection for malaria elimination in southern Zambia: a cross-sectional study
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Bhondoekhan, Fiona R. P., Searle, Kelly M., Hamapumbu, Harry, Mukuma Lubinda, Japhet Matoba, Musonda, Michael, Katowa, Ben, Shields, Timothy M., Kobayashi, Tamaki, Norris, Douglas E., Curriero, Frank C., Stevenson, Jennifer C., Thuma, Philip E., and Moss, William J.
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Additional file 1: Table S1. QIC goodness-of-fit statistics for the GEE models.
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- 2020
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16. Improving the efficiency of reactive case detection for malaria elimination in southern Zambia: a cross-sectional study
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Bhondoekhan, Fiona R.P., primary, Searle, Kelly M., additional, Hamapumba, Harry, additional, Lubinda, Mukuma, additional, Matoba, Japhet, additional, Musonda, Michael, additional, Katowa, Ben, additional, Shields, Timothy M., additional, Kobayashi, Tamaki, additional, Norris, Douglas, additional, Curriero, Frank C, additional, Stevenson, Jennifer C., additional, Thuma, Philip E., additional, and Moss, William J., additional
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- 2020
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17. Genetic Evidence of FocalPlasmodium falciparumTransmission in a Pre-elimination Setting in Southern Province, Zambia
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Pringle, Julia C, primary, Tessema, Sofonias, additional, Wesolowski, Amy, additional, Chen, Anna, additional, Murphy, Maxwell, additional, Carpi, Giovanna, additional, Shields, Timothy M, additional, Hamapumbu, Harry, additional, Searle, Kelly M, additional, Kobayashi, Tamaki, additional, Katowa, Ben, additional, Musonda, Michael, additional, Stevenson, Jennifer C, additional, Thuma, Philip E, additional, Greenhouse, Bryan, additional, Moss, William J, additional, and Norris, Douglas E, additional
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- 2018
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18. Improving the efficiency of reactive case detection for malaria elimination in southern Zambia: a cross-sectional study.
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Bhondoekhan, Fiona R. P., Searle, Kelly M., Hamapumbu, Harry, Lubinda, Mukuma, Matoba, Japhet, Musonda, Michael, Katowa, Ben, Shields, Timothy M., Kobayashi, Tamaki, Norris, Douglas E., Curriero, Frank C., Stevenson, Jennifer C., Thuma, Philip E., and Moss, William J.
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MALARIA ,ENVIRONMENTAL risk ,GENERALIZED estimating equations ,GLUCOSE-6-phosphate dehydrogenase deficiency ,HEALTH facilities ,CROSS-sectional method - Abstract
Background: Reactive case detection (RCD) seeks to enhance malaria surveillance and control by identifying and treating parasitaemic individuals residing near index cases. In Zambia, this strategy starts with passive detection of symptomatic incident malaria cases at local health facilities or by community health workers, with subsequent home visits to screen-and-treat residents in the index case and neighbouring (secondary) households within a 140-m radius using rapid diagnostic tests (RDTs). However, a small circular radius may not be the most efficient strategy to identify parasitaemic individuals in low-endemic areas with hotspots of malaria transmission. To evaluate if RCD efficiency could be improved by increasing the probability of identifying parasitaemic residents, environmental risk factors and a larger screening radius (250 m) were assessed in a region of low malaria endemicity. Methods: Between January 12, 2015 and July 26, 2017, 4170 individuals residing in 158 index and 531 secondary households were enrolled and completed a baseline questionnaire in the catchment area of Macha Hospital in Choma District, Southern Province, Zambia. Plasmodium falciparum prevalence was measured using PfHRP2 RDTs and quantitative PCR (qPCR). A Quickbird™ high-resolution satellite image of the catchment area was used to create environmental risk factors in ArcGIS, and generalized estimating equations were used to evaluate associations between risk factors and secondary households with parasitaemic individuals. Results: The parasite prevalence in secondary (non-index case) households was 0.7% by RDT and 1.8% by qPCR. Overall, 8.5% (n = 45) of secondary households had at least one resident with parasitaemia by qPCR or RDT. The risk of a secondary household having a parasitaemic resident was significantly increased in proximity to higher order streams and marginally with increasing distance from index households. The adjusted OR for proximity to third- and fifth-order streams were 2.97 (95% CI 1.04–8.42) and 2.30 (95% CI 1.04–5.09), respectively, and that for distance to index households for each 50 m was 1.24 (95% CI 0.98–1.58). Conclusion: Applying proximity to streams as a screening tool, 16% (n = 3) more malaria-positive secondary households were identified compared to using a 140-m circular screening radius. This analysis highlights the potential use of environmental risk factors as a screening strategy to increase RCD efficiency. [ABSTRACT FROM AUTHOR]
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- 2020
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19. Efficiency of a Malaria Reactive Test-and-Treat Program in Southern Zambia: A Prospective, Observational Study.
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Deutsch-Feldman, Molly, Hamapumbu, Harry, Lubinda, Jailos, Musonda, Michael, Katowa, Ben, Searle, Kelly M., Tamaki Kobayashi, Shields, Timothy M., Stevenson, Jennifer C., Thuma, Philip E., and Moss, William J.
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- 2018
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20. Distinct parasite populations infect individuals identified through passive and active case detection in a region of declining malaria transmission in southern Zambia.
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Searle, Kelly M., Katowa, Ben, Tamaki Kobayashi, Siame, Mwiche N. S., Mharakurwa, Sungano, Carpi, Giovanna, Norris, Douglas E., Stevenson, Jennifer C., Thuma, Philip E., and Moss, William J.
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ANTIMALARIALS , *DRUG resistance in bacteria , *EPIDEMIOLOGY , *PLASMODIUM falciparum , *SINGLE nucleotide polymorphisms ,MALARIA transmission - Abstract
Background: Substantial reductions in the burden of malaria have been documented in parts of sub-Saharan Africa, with elimination strategies and goals being formulated in some regions. Within this context, understanding the epidemiology of low-level malaria transmission is crucial to achieving and sustaining elimination. A 24 single-nucleotide-polymorphism Plasmodium falciparum molecular barcode was used to characterize parasite populations from infected individuals identified through passive and active case detection in an area approaching malaria elimination in southern Zambia. Methods: The study was conducted in the catchment area of Macha Hospital in Choma District, Southern Province, Zambia, where the parasite prevalence declined over the past decade, from 9.2% in 2008 to less than 1% in 2013. Parasite haplotypes from actively detected, P. falciparum-infected participants enrolled in a serial cross-sectional, community-based cohort study from 2008 to 2013 and from passively detected, P. falciparum-infected individuals enrolled at five rural health centres from 2012 to 2015 were compared. Changes in P. falciparum genetic relatedness, diversity and complexity were analysed as malaria transmission declined. Results: Actively detected cases identified in the community were most commonly rapid diagnostic test negative, asymptomatic and had submicroscopic parasitaemia. Phylogenetic reconstruction using concatenated 24 SNP barcode revealed a separation of parasite haplotypes from passively and actively detected infections, consistent with two genetically distinct parasite populations. For passively detected infections identified at health centres, the proportion of detectable polyclonal infections was consistently low in all seasons, in contrast with actively detected infections in which the proportion of polyclonal infections was high. The mean genetic divergence for passively detected infections was 34.5% for the 2012-2013 transmission season, 37.8% for the 2013-2014 season, and 30.8% for the 2014-2015 season. The mean genetic divergence for actively detected infections was 22.3% in the 2008 season and 29.0% in the 2008-2009 season and 9.9% across the 2012-2014 seasons. Conclusions: Distinct parasite populations were identified among infected individuals identified through active and passive surveillance, suggesting that infected individuals detected through active surveillance may not have contributed substantially to ongoing transmission. As parasite prevalence and diversity within these individuals declined, resource-intensive efforts to identify the chronically infected reservoir may not be necessary to eliminate malaria in this setting. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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21. MOESM2 of Distinct parasite populations infect individuals identified through passive and active case detection in a region of declining malaria transmission in southern Zambia
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Searle, Kelly, Katowa, Ben, Kobayashi, Tamaki, Mwiche Siame, Sungano Mharakurwa, Carpi, Giovanna, Norris, Douglas, Stevenson, Jennifer, Thuma, Philip, and Moss, William
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3. Good health - Abstract
Additional file 2. The modified â SNP Ď â used to account for missing data and mixed allele calls was calculated as follows.
22. MOESM2 of Distinct parasite populations infect individuals identified through passive and active case detection in a region of declining malaria transmission in southern Zambia
- Author
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Searle, Kelly, Katowa, Ben, Kobayashi, Tamaki, Mwiche Siame, Sungano Mharakurwa, Carpi, Giovanna, Norris, Douglas, Stevenson, Jennifer, Thuma, Philip, and Moss, William
- Subjects
3. Good health - Abstract
Additional file 2. The modified â SNP Ď â used to account for missing data and mixed allele calls was calculated as follows.
23. Temporal genomic analysis of Plasmodium falciparum reveals increased prevalence of mutations associated with delayed clearance following treatment with artemisinin-lumefantrine in Choma District, Southern Province, Zambia.
- Author
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Fola AA, Kobayashi T, Shields T, Hamapumbu H, Musonda M, Katowa B, Matoba J, Stevenson JC, Norris DE, Thuma PE, Wesolowski A, Moss WJ, Juliano JJ, and Bailey JA
- Abstract
The emergence of antimalarial drug resistance is an impediment to malaria control and elimination in Africa. Analysis of temporal trends in molecular markers of resistance is critical to inform policy makers and guide malaria treatment guidelines. In a low and seasonal transmission region of southern Zambia, we successfully genotyped 85.5% (389/455) of Plasmodium falciparum samples collected between 2013-2018 from 8 spatially clustered health centres using molecular inversion probes (MIPs) targeting key drug resistance genes. Aside from one sample carrying K13 R622 I , none of the isolates carried other World Health Organization-validated or candidate artemisinin partial resistance (ART-R) mutations in K13. However, 13% (CI, 9.6-17.2) of isolates had the AP2MU S160 N mutation, which has been associated with delayed clearance following artemisinin combination therapy in Africa. This mutation increased in prevalence between 2015-2018 and bears a genomic signature of selection. During this time period, there was an increase in the MDR1 N F D haplotype that is associated with reduced susceptibility to lumefantrine. Sulfadoxine-pyrimethamine polymorphisms were near fixation. While validated ART-R mutations are rare, a mutation associated with slow parasite clearance in Africa appears to be under selection in southern Zambia.
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- 2024
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24. RETRACTED: Gradual emergence followed by exponential spread of the SARS-CoV-2 Omicron variant in Africa.
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Fischer C, Maponga TG, Yadouleton A, Abílio N, Aboce E, Adewumi P, Afonso P, Akorli J, Andriamandimby SF, Anga L, Ashong Y, Beloufa MA, Bensalem A, Birtles R, Boumba ALM, Bwanga F, Chaponda M, Chibukira P, Chico RM, Chileshe J, Chongwe G, Cissé A, D'Alessandro U, de Lamballerie XN, de Morais JFM, Derrar F, Dia N, Diarra Y, Doumbia L, Drosten C, Dussart P, Echodu R, Eggers Y, Eloualid A, Faye O, Feldt T, Frühauf A, Halatoko A, Ilouga PV, Ismael N, Jambou R, Jarju S, Kamprad A, Katowa B, Kayiwa J, King'wara L, Koita O, Lacoste V, Lagare A, Landt O, Lekana-Douki SE, Lekana-Douki JB, Iipumbu E, Loemba H, Lutwama J, Mamadou S, Maman I, Manyisa B, Martinez PA, Matoba J, Mhuulu L, Moreira-Soto A, Mwangi J, N Dilimabaka N, Nassuna CA, Ndiath MO, Nepolo E, Njouom R, Nourlil J, Nyanjom SG, Odari EO, Okeng A, Ouoba JB, Owusu M, Owusu Donkor I, Phadu KK, Phillips RO, Preiser W, Ruhanya V, Salah F, Salifou S, Sall AA, Sylverken AA, Tagnouokam-Ngoupo PA, Tarnagda Z, Tchikaya FO, Tufa TB, and Drexler JF
- Abstract
The geographic and evolutionary origins of the SARS-CoV-2 Omicron variant (BA.1), which was first detected mid-November 2021 in Southern Africa, remain unknown. We tested 13,097 COVID-19 patients sampled between mid-2021 to early 2022 from 22 African countries for BA.1 by real-time RT-PCR. By November-December 2021, BA.1 had replaced the Delta variant in all African sub-regions following a South-North gradient, with a peak Rt of 4.1. Polymerase chain reaction and near-full genome sequencing data revealed genetically diverse Omicron ancestors already existed across Africa by August 2021. Mutations, altering viral tropism, replication and immune escape, gradually accumulated in the spike gene. Omicron ancestors were therefore present in several African countries months before Omicron dominated transmission. These data also indicate that travel bans are ineffective in the face of undetected and widespread infection.
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- 2022
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25. Sustained Malaria Transmission despite Reactive Screen-and-Treat in a Low-Transmission Area of Southern Zambia.
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Searle KM, Katowa B, Musonda M, Pringle JC, Hamapumbu H, Matoba J, Lubinda M, Shields T, Kobayashi T, Stevenson JC, Norris DE, Thuma PE, Wesolowski A, and Moss WJ
- Subjects
- Adolescent, Adult, Animals, Anopheles parasitology, Child, Child, Preschool, Cross-Sectional Studies, Family Characteristics, Female, Genotype, Humans, Malaria, Falciparum drug therapy, Malaria, Falciparum epidemiology, Male, Mass Screening statistics & numerical data, Parasitemia, Prevalence, Young Adult, Zambia epidemiology, Antimalarials therapeutic use, Malaria, Falciparum diagnosis, Malaria, Falciparum transmission, Mass Screening standards, Plasmodium falciparum genetics
- Abstract
Malaria elimination strategies are designed to more effectively identify and treat infected individuals to interrupt transmission. One strategy, reactive screen-and-treat, starts with passive detection of symptomatic cases at health facilities. Individuals residing within the index case and neighboring households are screened with a malaria rapid diagnostic test (RDT) and treated if positive. However, it is unclear to what extent this strategy is effective in reducing transmission. Reactive screen-and-treat was implemented in Choma district, Southern Province, Zambia, in 2013, in which residents of the index case and neighboring households within 140 m were screened with an RDT. From March 2016 to July 2018, the screening radius was extended to 250-m, and additional follow-up visits at 30 and 90 days were added to evaluate the strategy. Plasmodium falciparum parasite prevalence was measured using an RDT and by quantitative PCR (qPCR). A 24-single nucleotide polymorphism molecular bar-code assay was used to genotype parasites. Eighty-four index case households with 676 residents were enrolled between March 2016 and March 2018. Within each season, parasite prevalence declined significantly in index households at the 30-day visit and remained low at the 90-day visit. However, parasite prevalence was not reduced to zero. Infections identified by qPCR persisted between study visits and were not identified by RDT. Parasites identified within the same household were most genetically related; however, overall parasite relatedness was low and similar across time and space. Thus, despite implementation of a reactive screen-and-treat program, parasitemia was not eliminated, and persisted in targeted households for at least 3 months.
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- 2020
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