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444 results on '"Kawasaki, Nana"'

2. Nogo Receptor Antagonist LOTUS Promotes Neurite Outgrowth through Its Interaction with Teneurin-4.

Author

Kurihara, Yuji, Kawaguchi, Yuki, Ohta, Yuki, Kawasaki, Nana, Fujita, Yuki, and Takei, Kohtaro

Subjects
CENTRAL nervous system, LIQUID chromatography-mass spectrometry, CENTRAL nervous system injuries, NOGO protein, CELL adhesion molecules, CELL adhesion, NERVOUS system regeneration, AXONS
Abstract

Simple Summary: Neuronal growth is an essential process for establishing functional neural networks in developing brain and restoring dysfunctional networks caused by injuries in the adult brain. In the adult central nervous system, injured neurons fail to re-extend their axons due to several axon growth inhibitors mainly expressed in glial cells. We previously reported that LOTUS protein helps injured adult neurons to regenerate by blocking the inhibitors. In this study, we found that LOTUS itself promotes axon growth through an independent mechanism from the blockade of the inhibitors. LOTUS directly binds to Teneurin-4, a cell adhesion molecule expressed on the surface of neurons and the binding promotes axon growth. Thus, LOTUS was found to have two functions in axon growth. This finding may provide a new insight into neural network formation in developing brain and neural regrowth mechanism following the adult injured brain. These functions of LOTUS protein are useful for developing new therapeutic approaches to promote nerve regeneration after injury in the adult central nervous system. Neurite outgrowth is a crucial process for organizing neuronal circuits in neuronal development and regeneration after injury. Regenerative failure in the adult mammalian central nervous system (CNS) is attributed to axonal growth inhibitors such as the Nogo protein that commonly binds to Nogo receptor-1 (NgR1). We previously reported that lateral olfactory tract usher substance (LOTUS) functions as an endogenous antagonist for NgR1 in forming neuronal circuits in the developing brain and improving axonal regeneration in the adult injured CNS. However, another molecular and cellular function of LOTUS remains unknown. In this study, we found that cultured retinal explant neurons extend their neurites on the LOTUS-coating substrate. This action was also observed in cultured retinal explant neurons derived from Ngr1-deficient mouse embryos, indicating that the promoting action of LOTUS on neurite outgrowth may be mediated by unidentified LOTUS-binding protein(s). We therefore screened the binding partner(s) of LOTUS by using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). LC-MS/MS analysis and pull-down assay showed that LOTUS interacts with Teneurin-4 (Ten-4), a cell adhesion molecule. RNAi knockdown of Ten-4 inhibited neurite outgrowth on the LOTUS substrate in retinoic acid (RA)-treated Neuro2A cells. Furthermore, a soluble form of Ten-4 attenuates the promoting action on neurite outgrowth in cultured retinal explant neurons on the LOTUS substrate. These results suggest that LOTUS promotes neurite outgrowth by interacting with Ten-4. Our findings may provide a new molecular mechanism of LOTUS to contribute to neuronal circuit formation in development and to enhance axonal regeneration after CNS injury. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Comparative Analysis of Site-Specific N-glycosylation of LAMP1 from Breast Cancer Tissues.

Author

Ohashi, Shoko, Takakura, Daisuke, Kobayashi, Noritoshi, Tokuhisa, Motohiko, Ichikawa, Yasushi, and Kawasaki, Nana

Subjects
GLYCANS, BREAST cancer, GLYCAN structure, PRINCIPAL components analysis, SIALIC acids, GLYCOPROTEIN analysis
Abstract

Glycosylation changes in cancer proteins have been associated with malignant transformation. However, techniques for analyzing site-specific glycosylation changes in target proteins obtained from clinical tissue samples are insufficient. To overcome these problems, we developed a targeted N-glycoproteomic approach consisting of immunoprecipitation, glycopeptide enrichment, LC/MS/MS and structural assignment using commercially available analytical software followed by manual confirmation. This approach was applied to the comparative site-specific glycosylation analysis of lysosome-associated membrane glycoprotein 1 (LAMP1) between breast cancer (BC) tumors and normal tissues adjacent to tumors. Extensive determination of glycan heterogeneity from four N-glycosylation sites (Asn84/103/249/261) in LAMP1 identified 262 glycoforms and revealed remarkable diversity in tumor glycan structures. A significant increase in N-glycoforms with multiple fucoses and sialic acids at Asn84/249 and high-mannose-type glycans at Asn103/261 were observed in the tumor. Principal component analysis revealed that tumors of different subtypes have independent distributions. This approach enables site-specific glycopeptide analysis of target glycoprotein in breast cancer tissue and become a powerful tool for characterizing tumors with different pathological features by their glycan profiles. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Antibody Pharmaceuticals

Author

Kawasaki, Nana, Hashii, Noritaka, Taniguchi, Naoyuki, editor, Endo, Tamao, editor, Hirabayashi, Jun, editor, Nishihara, Shoko, editor, Kadomatsu, Kenji, editor, Akiyoshi, Kazunari, editor, and Aoki-Kinoshita, Kiyoko F., editor

Published
2019
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10. Standard Definitions of Terms Related to Mass Spectrometry (Additional Edition 2023)

Author

Aoyagi, Satoka, primary, Kawasaki, Nana, additional, Kinumi, Tomoya, additional, Sato, Takaya, additional, Sugai, Toshiki, additional, Sumino, Hirochika, additional, Takeuchi, Takae, additional, Naito, Yasuhide, additional, Hirata, Takafumi, additional, Fukuyama, Mayuko, additional, Maruoka, Teruyuki, additional, Minami, Masayo, additional, Motoyama, Akira, additional, and Yoshino, Ken-ichi, additional

Published
2023
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15. Comparison of analytical methods for profiling N- and O-linked glycans from cultured cell lines: HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study

Author

Ito, Hiromi, Kaji, Hiroyuki, Togayachi, Akira, Azadi, Parastoo, Ishihara, Mayumi, Geyer, Rudolf, Galuska, Christina, Geyer, Hildegard, Kakehi, Kazuaki, Kinoshita, Mitsuhiro, Karlsson, Niclas G., Jin, Chunsheng, Kato, Koichi, Yagi, Hirokazu, Kondo, Sachiko, Kawasaki, Nana, Hashii, Noritaka, Kolarich, Daniel, Stavenhagen, Kathrin, Packer, Nicolle H., Thaysen-Andersen, Morten, Nakano, Miyako, Taniguchi, Naoyuki, Kurimoto, Ayako, Wada, Yoshinao, Tajiri, Michiko, Yang, Pengyuan, Cao, Weiqian, Li, Hong, Rudd, Pauline M., and Narimatsu, Hisashi

Published
2016
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16. Approaches to Quality Risk Management When Using Single-Use Systems in the Manufacture of Biologics

Author

Ishii-Watabe, Akiko, Hirose, Akihiko, Katori, Noriko, Hashii, Norikata, Arai, Susumu, Awatsu, Hirotoshi, Eiza, Akira, Hara, Yoshiaki, Hattori, Hideshi, Inoue, Tomomi, Isono, Tetsuya, Iwakura, Masahiro, Kajihara, Daisuke, Kasahara, Nobuo, Matsuda, Hiroyuki, Murakami, Sei, Nakagawa, Taishiro, Okumura, Takehiro, Omasa, Takeshi, Takuma, Shinya, Terashima, Iyo, Tsukahara, Masayoshi, Tsutsui, Maiko, Yano, Takahiro, and Kawasaki, Nana

Published
2015
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17. Non-classical glycosylation determines intracellular trafficking of APP and Aβ production

Author

Kitazume, Shinobu, primary, Tachida, Yuriko, additional, Iijima, Junko, additional, Takahashi, Kazuto, additional, Suzuki, Hideaki, additional, Yamaguchi, Yoshiki, additional, Tanaka, katsunori, additional, Nakano, Miyako, additional, Takakura, Daisuke, additional, Kawasaki, Nana, additional, Saito, Yuko, additional, Manya, Hiroshi, additional, and Endo, Tamao, additional

Published
2022
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19. International nonproprietary names for monoclonal antibodies: an evolving nomenclature system

Author

Guimaraes Koch, Sofia S., primary, Thorpe, Robin, additional, Kawasaki, Nana, additional, Lefranc, Marie-Paule, additional, Malan, Sarel, additional, Martin, Andrew C.R., additional, Mignot, Gilles, additional, Plückthun, Andreas, additional, Rizzi, Menico, additional, Shubat, Stephanie, additional, Weisser, Karin, additional, and Balocco, Raffaella, additional

Published
2022
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25. LC/MS of N-Linked Oligosaccharides

Author

Kawasaki, Nana, Itoh, Satsuki, Yamaguchi, Teruhide, Taniguchi, Naoyuki, editor, Suzuki, Akemi, editor, Ito, Yukishige, editor, Narimatsu, Hisashi, editor, Kawasaki, Toshisuke, editor, and Hase, Sumihiro, editor

Published
2008
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28. Online MS Colloquium by Kanto, Hokkaido, Tohoku and Chubu-Area Groups, and Diversity and Inclusion Committee: Lecture on Quantitative Analysis, Lab Tour, and Social Meeting

Author

Asanuma, Miwako, primary, Kawasaki, Nana, additional, Kobayashi, Yuki, additional, Saikusa, Kazumi, additional, Sawa, Ryuichi, additional, Shimbo, Kazutaka, additional, Tanabe, Kana, additional, Nozawa, Kohei, additional, Harazono, Akira, additional, Motoyama, Akira, additional, Akutsu, Hiroaki, additional, Oka, Seiko, additional, Jin, Shigeki, additional, Takata, Yusuke, additional, Bajo, Ken-ichi, additional, Hirose, Tomohiro, additional, Mori, Ayako, additional, Saigusa, Daisuke, additional, Sato, Emiko, additional, Soga, Tomoyoshi, additional, Hirayama, Akiyoshi, additional, Maekawa, Masamitsu, additional, Mano, Nariyasu, additional, Yoshino, Ken-ichi, additional, Osaka, Issey, additional, Kurono, Sadamu, additional, Kuwata, Keiko, additional, Sakai, Tatsuko, additional, Setou, Mitsutoshi, additional, Takahashi, Yutaka, additional, Naito, Yasuhide, additional, and Matsumoto, Tomohiro, additional

Published
2022
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29. Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

Author

Togo, Kazuyuki, primary, Fukusumi, Hayato, additional, Shofuda, Tomoko, additional, Ohnishi, Hiroshi, additional, Yamazaki, Hiroyuki, additional, Hayashi, Mariko Kato, additional, Kawasaki, Nana, additional, Takei, Nobuyuki, additional, Nakazawa, Takanobu, additional, Saito, Yumiko, additional, Baba, Kousuke, additional, Hashimoto, Hitoshi, additional, Sekino, Yuko, additional, Shirao, Tomoaki, additional, Mochizuki, Hideki, additional, and Kanemura, Yonehiro, additional

Published
2021
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30. Genetic Polymorphisme of FCGRT Encoding FcRn in a Japanese Population and Their Functional Analysis

Author

Ishii-Watabe, Akiko, Saito, Yoshiro, Suzuki, Takuo, Tada, Minoru, Ukaji, Maho, Maekawa, Keiko, Kurose, Kouichi, Kaniwa, Nahoko, Sawada, Jun-ichi, Kawasaki, Nana, Yamaguchi, Teruhide, Eguchi Nakajima, Takako, Kato, Ken, Yamada, Yasuhide, Shimada, Yasuhiro, Yoshida, Teruhiko, Ura, Takashi, Saito, Miyuki, Muro, Kei, Doi, Toshihiko, Fuse, Nozomu, Yoshino, Takayuki, Ohtsu, Atsushi, Saijo, Nagahiro, Hamaguchi, Tetsuya, Okuda, Haruhiro, and Matsumura, Yasuhiro

Published
2010
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32. Glycan Epitopes on 201B7 Human Induced Pluripotent Stem Cells using R-10G and R-17F Marker Antibodies

Author

Nagai, Yuko, Nakao, Hiromi, Kojima, Aya, Komatsubara, Yuka, Ohta, Yuki, Kawasaki, Nana, Kawasaki, Nobuko, Toyoda, Hidenao, and Kawasaki, Toshisuke

Subjects
biochemistry
Abstract

We developed two human induced pluripotent stem cell (hiPSC)/human embryonic stem cell-specific glycan-recognizing mouse antibodies, R-10G and R-17F, using the Tic (JCRB1331) hiPSC line as an antigen. R-10G recognizes a low-sulfate keratan sulfate, and R-17F recognizes lacto-N-fucopentaose-1. To evaluate the general characteristics of stem cell glycans, we used the hiPSC line 201B7 (HPS0063), a prototype iPSC line. Using an R-10G affinity column, an R-10G-binding protein was isolated. The protein yielded a single but very broad band from 480 to 1,236 kDa by blue native gel electrophoresis. After trypsin digestion, the protein was identified as podocalyxin by liquid chromatography/mass spectrometry. According to Western blotting, the protein reacted with R-10G and R-17F. The R-10G positive band was resistant to digestion with glycan-degrading enzymes, including peptide N-glycanase, but the intensity of the band was decreased significantly by digestion with keratanase, keratanase II, and endo-β-galactosidase, suggesting the R-10G epitope to be a keratan sulfate. These results suggest that keratan sulfate-type epitopes are shared by hiPSCs. However, the keratan sulfate from 201B7 cells contained a polylactosamine disaccharide unit (Galβ1-4GlcNAc) at a significant frequency, whereas that from Tic cells consisted mostly of keratan sulfate disaccharide units (Galβ1-4GlcNAc(6S)). In addition, the abundance of the R-10G epitope was significantly lower in 201B7 cells than in Tic cells.

Published
2021

33. Additional file 1 of Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

Author

Togo, Kazuyuki, Fukusumi, Hayato, Shofuda, Tomoko, Ohnishi, Hiroshi, Yamazaki, Hiroyuki, Hayashi, Mariko Kato, Kawasaki, Nana, Takei, Nobuyuki, Nakazawa, Takanobu, Saito, Yumiko, Baba, Kousuke, Hashimoto, Hitoshi, Sekino, Yuko, Shirao, Tomoaki, Mochizuki, Hideki, and Kanemura, Yonehiro

Subjects
animal diseases, virus diseases
Abstract

Additional file 1: Table 1. Primers for SYBR Green-based qRT-PCR.

Published
2021
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34. Additional file 3 of Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

Author

Togo, Kazuyuki, Fukusumi, Hayato, Shofuda, Tomoko, Ohnishi, Hiroshi, Yamazaki, Hiroyuki, Hayashi, Mariko Kato, Kawasaki, Nana, Takei, Nobuyuki, Nakazawa, Takanobu, Saito, Yumiko, Baba, Kousuke, Hashimoto, Hitoshi, Sekino, Yuko, Shirao, Tomoaki, Mochizuki, Hideki, and Kanemura, Yonehiro

Abstract

Additional file 3: Table 3. Antibodies used in Western bold analysis.

Published
2021
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35. Additional file 4 of Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

Author

Togo, Kazuyuki, Fukusumi, Hayato, Shofuda, Tomoko, Ohnishi, Hiroshi, Yamazaki, Hiroyuki, Hayashi, Mariko Kato, Kawasaki, Nana, Takei, Nobuyuki, Nakazawa, Takanobu, Saito, Yumiko, Baba, Kousuke, Hashimoto, Hitoshi, Sekino, Yuko, Shirao, Tomoaki, Mochizuki, Hideki, and Kanemura, Yonehiro

Subjects
nervous system
Abstract

Additional file 4: Figure S1. Gene expression analysis of pre- and postsynaptic markers during the neuronal differentiation of hiPSC-NPCs in the absence and presence of NGF. Dot and box plots showing the expression of postsynaptic markers (drebrin A+E and drebrin A) and a presynaptic marker (synaptophysin) during neuronal differentiation. Statistical analysis was performed using ANOVA with the post hoc Tukey–Kramer method and Student’s t-tests (***p

Published
2021
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36. Additional file 2 of Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

Author

Togo, Kazuyuki, Fukusumi, Hayato, Shofuda, Tomoko, Ohnishi, Hiroshi, Yamazaki, Hiroyuki, Hayashi, Mariko Kato, Kawasaki, Nana, Takei, Nobuyuki, Nakazawa, Takanobu, Saito, Yumiko, Baba, Kousuke, Hashimoto, Hitoshi, Sekino, Yuko, Shirao, Tomoaki, Mochizuki, Hideki, and Kanemura, Yonehiro

Abstract

Additional file 2: Table 2. Antibodies used in this study.

Published
2021
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37. Genetic polymorphisms of FCGR2A encoding Fcγ receptor IIa in a Japanese population and functional analysis of the L273P variant

Author

Tada, Minoru, Ishii-Watabe, Akiko, Maekawa, Keiko, Fukushima-Uesaka, Hiromi, Kurose, Kouichi, Suzuki, Takuo, Kaniwa, Nahoko, Sawada, Jun-ichi, Kawasaki, Nana, Nakajima, Takako Eguchi, Kato, Ken, Yamada, Yasuhide, Shimada, Yasuhiro, Yoshida, Teruhiko, Ura, Takashi, Saito, Miyuki, Muro, Kei, Doi, Toshihiko, Fuse, Nozomu, Yoshino, Takayuki, Ohtsu, Atsushi, Saijo, Nagahiro, Okuda, Haruhiro, Hamaguchi, Tetsuya, Saito, Yoshiro, and Matsumura, Yasuhiro

Published
2012
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50. Monitoring the glycosylation of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole‐propionate‐type glutamate receptors using specific antibodies reveals a novel regulatory mechanism of N‐glycosylation occupancy by molecular chaperones in mice

Author

Midorikawa, Ryosuke, primary, Takakura, Daisuke, additional, Morise, Jyoji, additional, Wakazono, Yoshihiko, additional, Kawasaki, Nana, additional, Oka, Shogo, additional, and Takamiya, Kogo, additional

Published
2020
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