67 results on '"Kefan Zhang"'
Search Results
2. Study on the use of 3D printed guides in the individualized reconstruction of the anterior cruciate ligament
- Author
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Xin Wang, Dening Wang, Chenchen Zhang, Kefan Zhang, Changling Du, and Hui Shi
- Subjects
Anterior cruciate ligament ,Anatomical positioning ,Guide ,Anatomical reconstruction ,3D printing ,3D reconstruction ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Objective Evaluation of the accuracy and effectiveness of 3D printed guides to assist femoral tunnel preparation in individualised reconstruction of the anterior cruciate ligament. Methods Sixty patients who attended the Affiliated Hospital of Binzhou Medical College for autologous hamstring single bundle reconstruction of the anterior cruciate ligament from October 2018 to October 2020 were selected and randomly divided into two groups, including 31 cases in the 3D printing group (14 males and 17 females, mean age 41.94 ± 10.15 years) and 29 cases in the control group (13 males and 16 females, mean age 37.76 ± 10.34 years). Patients in both groups were assessed for intraoperative femoral tunnel accuracy, the number of intraoperative positioning and the time taken to prepare the femoral tunnel, the length of the anteromedial approach incision, the pre-planned bone tunnel length and intraoperative bone tunnel length in the 3D printed group, IKDC score and Lysholm score preoperatively and at 3, 6 and 12 months postoperatively, the Lachman、pivot-shift test preoperatively and at 6 months postoperatively, gait analysis to assess internal and external rotation in flexion of the knee at 12 months postoperatively and postoperative complications in both groups. Results There was no statistical difference in functional knee scores and anteromedial approach incision length between the 3D printed and control groups (p > 0.05), while there was a statistical difference in the accuracy of tunnel positioning, the time taken to prepare the femoral bone tunnel and the degree of external rotation of the knee in flexion between the two groups (p 0.05). Complications: One case in the 3D printing group developed intermuscular vein thrombosis in the affected lower limb after surgery, which disappeared after treatment, while three cases in the control group developed intermuscular vein thrombosis in the affected lower limb. No complications such as bone tunnel rupture, deep vein thrombosis in the lower limb and infection occurred in either group. Conclusion 3D printed guides assisted with individualized ACL reconstruction may improve the accuracy of femoral tunnel positioning, which is safe and effective, while reducing the operative time and the number of intraoperative positioning, without increasing the length of incision, and may obtain higher functional scores and rotational stability of the knee joint, which is in line with the concept of individualized ACL reconstruction.
- Published
- 2024
- Full Text
- View/download PDF
3. Circular RNA PRKCI (hsa_circ_0067934): a potential target in the pathogenesis of human malignancies
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Shipei Qiu, Kefan Zhang, Siyu Chen, and Shuting Yin
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CircPRKCI ,malignant tumor ,molecular target ,overall survival ,invasion ,migration and proliferation ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Circular RNAs (circRNAs) are a new type of endogenous non-coding RNA formed by a covalent closed loop. CircRNAs are characterized by specificity, universality, conservation, and stability. They are abundant in eukaryotic cells and have biological regulatory roles at various transcriptional and post-transcriptional levels. The upregulation of circPRKCI has been observed in a variety of tumors and is directly related to the clinicopathological characteristics of tumors and prognosis. More importantly, circPRKCI can participate in the tumorigenesis, progression, recurrence, and metastasis of various tumors through many functional mechanisms, including the activation of signaling pathways, such as the phosphatidylinositol-3-kinase (PI3K)/AKT pathway, and sponging of many microRNAs (miRNAs). This review summarizes the progress achieved in understanding the biological functions of circRNA PRKCI in various tumors. The goal is to inform the discovery of more functional mechanisms and new anticancer molecular targets.
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- 2024
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4. A Method for Land Vehicle Gravity Anomaly Measurement Combining an Inertial Navigation System, Odometer, and Geo-Information System
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Kefan Zhang, Junyang Zhao, and Zhili Zhang
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land vehicle gravity anomaly measurement ,odometer (OD) ,integrated navigation ,geo-information system (GIS) ,gravity aided navigation ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Transportation engineering ,TA1001-1280 - Abstract
Land vehicle gravity anomaly measurement relies heavily on global navigation satellite system (GNSS). However, when gravity measurement is carried out in special environments such as forests, valleys and tunnels, GNSS observation quality will inevitably decline, which directly affects the accuracy of gravity anomaly measurement. From the point of view of the gravity anomaly measurement principle, obtaining accurate elevation information of the test line is the premise to ensure the accuracy of gravity anomaly measurement. Thus, this paper proposes a strapdown land vehicle dynamic gravity anomaly measurement method combining an odometer and a geo-information system. In this method, strapdown inertial navigation errors are suppressed by observing the velocity of the odometer output. Then, the position information obtained by the combined navigation is entered into the geo-information system to obtain the elevation. The results of a single test line show that the external coincidence accuracy of the proposed method is 1.65 mGal, and the accuracy is comparable to the traditional GNSS assisted land vehicle gravimetry method. In addition, compared with the odometer assisted land vehicle gravimetry method, the external coincidence accuracy is increased by 30%.
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- 2024
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5. Effect of Replacing Alfalfa Hay with Common Vetch Hay in Sheep Diets on Growth Performance, Rumen Fermentation and Rumen Microbiota
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Chunhuan Ren, Xiaoan Zhang, Huiqing Wei, Sunze Wang, Wenjie Wang, Li He, Yuan Lu, Kefan Zhang, Zijun Zhang, Guanjun Wang, and Yafeng Huang
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common vetch ,growth performance ,rumen fermentation ,microbiota composition ,sheep ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
The aim of this study was to determine whether the inclusion of 40% of common vetch (CV) hay as a feed ingredient in place of alfalfa hay (AH) would improve performance and ruminal fermentation and microbiota in fattening lambs. Twenty lambs were equally divided into two groups: control group (fed 40% AH with 20% rice straw) and CV group (fed 40% CV hay with 20% rice straw). Concerning hay quality, CV hay had greater in vitro digestibility of dry matter and neutral detergent fiber (p < 0.05) than AH. Lambs fed the CV diet had a higher average daily gain (ADG) and efficiency of feed and economy than lambs fed the control group. The NH3-N content and estimated methane produced per unit of ADG of the CV diet group were significantly lower (p < 0.05) than control group. Multiple differential microbial genera were identified, with Prevotella being the most dominant genus and a tendency towards higher (p = 0.095) in lambs offered CV diet. The higher Ruminococcus abundance (p < 0.05) was found in animals of the CV group compared to the control group. In summary, CV can be incorporated into lamb diets as a low-cost forage alternative to AH to improve feed efficiency and animal performance and to reduce methane produced per unit of ADG.
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- 2024
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6. Failure similarity of gravity dam under downstream contacting blast loading
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Kefan Zhang, Xiangyu Li, Yong Peng, Fangyun Lu, and Zhandong Tian
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Similarity law ,Contact blast test ,Gravity dam ,Fracture ,Crack path ,Technology - Abstract
The analysis of the blast resistance and dynamic responses of gravity dams under extreme loading conditions is crucial in ensuring the safety of hydraulic engineering structures. Recent international conflicts have further emphasized the importance of research in this field. However, the lack of quantitative experimental studies and analysis methods for assessing prototype dam damage has posed significant challenges. To address this gap, experiments and numerical simulations on scaled concrete models were conducted, considering two equivalent detonation loads and four scaling ratios. Utilizing a non-dimensional approach, comprehensive comparisons of the dam's initial fracture locations, fracture paths, and extent of damage were performed, thus leading to the identification of consistent damage patterns and similarities between the different scaled models. The results demonstrated that the primary failure mode caused by the detonation loads on the downstream face was the overall fracture of the dam crest, with a slight downward shift in the initial crack position as the detonation load increased. Remarkably, the fracture paths exhibited satisfying similarities across the different scaling ratios. Furthermore, through further discussion on topics such as the initial fracture locations and the influence of reservoir water, both theoretical and practical insights were obtained for investigating the damage of prototype dams based on similarity laws.
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- 2024
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7. Direct Yaw Moment Control for Distributed Drive Electric Vehicles Based on Hierarchical Optimization Control Framework
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Jie Hu, Kefan Zhang, Pei Zhang, and Fuwu Yan
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4W-DDEV ,direct yaw moment control ,torque distribution ,linear quadratic regulator ,IWCPSO ,Mathematics ,QA1-939 - Abstract
Direct yaw moment control (DYC) can effectively improve the yaw stability of four-wheel distributed drive electric vehicles (4W-DDEVs) under extreme conditions, which has become an indispensable part of active safety control for 4W-DDEVs. This study proposes a novel hierarchical DYC architecture for 4W-DDEVs to enhance vehicle stability during ever-changing road conditions. Firstly, a vehicle dynamics model is established, including a two-degree-of-freedom (2DOF) vehicle model for calculating the desired yaw rate and sideslip angle as the control target of the upper layer controller, a DDEV model composed of a seven-degree-of-freedom (7DOF) vehicle model, a tire model, a motor model and a driver model. Secondly, a hierarchical DYC is designed combining the upper layer yaw moment calculation and low layer torque distribution. Specifically, based on Matlab/Simulink, improved linear quadratic regulator (LQR) with weight matrix optimization based on inertia weight cosine-adjustment particle swarm optimization (IWCPSO) is employed to compute the required additional yaw moment in the upper-layer controller, while quadratic programming (QP) is used to allocate four motors’ torque with the optimization objective of minimizing the tire utilization rate. Finally, a comparative test with double-lane-change and sinusoidal conditions under a low and high adhesion road surface is conducted on Carsim and Matlab/Simulink joint simulation platform. With IWCPSO-LQR under double-lane-change (DLC) condition on a low adhesion road surface, the yaw rate and sideslip angle of the DDEV exhibits improvements of 95.2%, 96.8% in the integral sum of errors, 94.9%, 95.1% in the root mean squared error, and 78.8%, 98.5% in the peak value compared to those without control. Simulation results indicate the proposed hierarchical control method has a remarkable control effect on the yaw rate and sideslip angle, which effectively strengthens the driving stability of 4W-DDEVs.
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- 2024
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8. Electromagnetic-thermal two-way coupling analysis and application on helium-cooled solid blanket
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Kefan Zhang, Shuai Wang, and Hongli Chen
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HCSB ,EM-Thermal Coupling ,MD event ,Nuclear engineering. Atomic power ,TK9001-9401 - Abstract
The blanket plays an important role in fusion reactor and stands extremely high thermal and electromagnetic loads during operation situation and plasma disruption event, brings the need for precise thermal and electromagnetic analysis. Since the thermal field and EM field interact with each other nonlinearly, we develop a method of electromagnetic-thermal two-way coupling by using finite element software COMSOL. The coupling analyses of blanket under steady state and MD event are implemented and the results are analyzed. For steady state, the influences of coupling effects are relatively small but still recommended to be considered for a high precision analysis. The influence of thermal field on EM field can't be ignored under MD events. The variation of force density could cause a significant change in stress in certain parts of blanket. The influence of Joule heat during MD event is negligible, yet the potential temperature rise caused by induced current after MD event still needs to be researched.
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- 2023
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9. Correlation between stem cell molecular phenotype and atherosclerotic plaque neointima formation and analysis of stem cell signal pathways
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Chuanxin Shi, Kefan Zhang, Zhenyu Zhao, Yifan Wang, Haozhe Xu, and Wei Wei
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stem cells ,stem cell markers ,molecular phenotype ,neointima ,atherosclerotic disease ,signaling pathways ,Biology (General) ,QH301-705.5 - Abstract
Vascular stem cells exist in the three-layer structure of blood vessel walls and play an indispensable role in angiogenesis under physiological conditions and vascular remodeling under pathological conditions. Vascular stem cells are mostly quiescent, but can be activated in response to injury and participate in endothelial repair and neointima formation. Extensive studies have demonstrated the differentiation potential of stem/progenitor cells to repair endothelium and participate in neointima formation during vascular remodeling. The stem cell population has markers on the surface of the cells that can be used to identify this cell population. The main positive markers include Stem cell antigen-1 (Sca1), Sry-box transcription factor 10 (SOX10). Stromal cell antigen 1 (Stro-1) and Stem cell growth factor receptor kit (c-kit) are still controversial. Different parts of the vessel have different stem cell populations and multiple markers. In this review, we trace the role of vascular stem/progenitor cells in the progression of atherosclerosis and neointima formation, focusing on the expression of stem cell molecular markers that occur during neointima formation and vascular repair, as well as the molecular phenotypic changes that occur during differentiation of different stem cell types. To explore the correlation between stem cell molecular markers and atherosclerotic diseases and neointima formation, summarize the differential changes of molecular phenotype during the differentiation of stem cells into smooth muscle cells and endothelial cells, and further analyze the signaling pathways and molecular mechanisms of stem cells expressing different positive markers participating in intima formation and vascular repair. Summarizing the limitations of stem cells in the prevention and treatment of atherosclerotic diseases and the pressing issues that need to be addressed, we provide a feasible scheme for studying the signaling pathways of vascular stem cells involved in vascular diseases.
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- 2023
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10. A Novel Variable Selection Method Based on Binning-Normalized Mutual Information for Multivariate Calibration
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Liang Zhong, Ruiqi Huang, Lele Gao, Jianan Yue, Bing Zhao, Lei Nie, Lian Li, Aoli Wu, Kefan Zhang, Zhaoqing Meng, Guiyun Cao, Hui Zhang, and Hengchang Zang
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variable selection ,near-infrared spectroscopy ,data binning ,normalized mutual information ,Organic chemistry ,QD241-441 - Abstract
Variable (wavelength) selection is essential in the multivariate analysis of near-infrared spectra to improve model performance and provide a more straightforward interpretation. This paper proposed a new variable selection method named binning-normalized mutual information (B-NMI) based on information entropy theory. “Data binning” was applied to reduce the effects of minor measurement errors and increase the features of near-infrared spectra. “Normalized mutual information” was employed to calculate the correlation between each wavelength and the reference values. The performance of B-NMI was evaluated by two experimental datasets (ideal ternary solvent mixture dataset, fluidized bed granulation dataset) and two public datasets (gasoline octane dataset, corn protein dataset). Compared with classic methods of backward and interval PLS (BIPLS), variable importance projection (VIP), correlation coefficient (CC), uninformative variables elimination (UVE), and competitive adaptive reweighted sampling (CARS), B-NMI not only selected the most featured wavelengths from the spectra of complex real-world samples but also improved the stability and robustness of variable selection results.
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- 2023
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11. Valorizing carbon dioxide via electrochemical reduction on gas‐diffusion electrodes
- Author
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Yuqing Luo, Kefan Zhang, Yanguang Li, and Yuhang Wang
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carbon dioxide electroreduction ,gas‐diffusion electrodes ,reactors ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Information technology ,T58.5-58.64 - Abstract
Abstract The electrochemical carbon dioxide (CO2) reduction provides a means to upgrade CO2 into value‐added chemicals. When powered by renewable electricity, CO2 electroreduction holds the promise of chemical manufacturing with carbon neutrality. A commercially relevant CO2 electroreduction process should be highly selective and productive toward desired products, energetically efficient for power conversion, and stable for long‐term operation. To achieve these goals, designing gas‐diffusion catalytic electrodes and prototyping reactors built upon in‐depth understandings of the reaction mechanisms are of paramount importance. In this review, the fundamentals of gas‐diffusion electrodes are briefly presented. Then, the most recent advances in developing high‐performance CO2 reduction using gas‐diffusion electrodes are overviewed. Reactor engineering aiming at enhancing productivity, energy efficiency, CO2 single‐pass utilization, and operating lifetime is further discussed. Challenges in developing CO2 electroreduction systems are included. The prospects for advancing CO2 electroreduction toward practical applications are also narrated.
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- 2021
- Full Text
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12. Effect of packing structure on anisotropic effective thermal conductivity of thin ceramic pebble bed
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Shuang Wang, Shuai Wang, Bowen Wu, Yuelin Lu, Kefan Zhang, and Hongli Chen
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Effective thermal conductivity ,Anisotropy ,Pebble bed ,Discrete element method ,Three-dimensional thermal network method ,Nuclear engineering. Atomic power ,TK9001-9401 - Abstract
Helium cooled solid breeder blanket as an important blanket candidate of the Tokamak fusion reactor uses ceramic pebble bed for tritium breeding. Considering the poor effective thermal conductivity of the ceramic breeder pebble bed, thin structure of tritium breeder pebble bed is usually adopted in the blanket design. The container wall has a great influence on the thin pebble bed packing structure, especially for the assembly of mono-sized particles, and thin pebble bed will appear anisotropic effective thermal conductivity phenomenon. In this paper, thin ceramic pebble beds composed of 1 mm diameter Li4SiO4 particles are generated by the EDEM 2.7. The effective thermal conductivity of different thickness pebble beds in the three-dimensional directions are analyzed by three-dimensional thermal network method. It is observed that thin Li4SiO4 pebble bed showing anisotropic effective thermal conductivity under the practical design size. Normally, the effective thermal conductivity along the bed vertical direction is higher than the horizontal direction due to the gravity effect. As the thickness increases from 10 mm to 40 mm, the effective thermal conductivity of the pebble bed gradually increases.
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- 2021
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13. Novel Similarity Methods Evaluation and Feasible Application for Pharmaceutical Raw Material Identification with Near-Infrared Spectroscopy
- Author
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Hui Zhang, Lian Li, Shuang Quan, Weilu Tian, Kefan Zhang, Lei Nie, and Hengchang Zang
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Chemistry ,QD1-999 - Published
- 2020
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14. Theoretical Analysis of Blast Protection of Graded Metal Foam-Cored Sandwich Cylinders/Rings
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Minzu Liang, Xiangyu Li, Yuliang Lin, Kefan Zhang, and Fangyun Lu
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blast protection ,sandwich structure ,metallic foams ,gradient ,cylinders ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
The blast resistance of a sandwich-walled cylinder/ring comprising two metal face-sheets and a graded metal foam core, subjected to internal air blast loading, is investigated. Analytical models are developed for the deformation of the sandwich cylinder with positive and negative gradient cores under internal blast loading. The deformation process is divided into three distinct phases, namely the fluid–structure interaction phase, core-crushing phase, and outer face-sheet deformation phase. Finite element modeling is performed using the Voronoi material model. The proposed analytical models are verified through finite element analysis, and reasonable agreement is observed between the analytical predictions and finite element results. The sandwich structures with high energy absorption capacity or low maximum radial deflection are satisfied for the protecting purpose of impact/blast resistance requirements. Typical deformation processes are classified and analyzed; the effects of explosive charge, face-sheet thickness, and core gradient on the structural response are also examined. The results indicate that both the deformation modes and the structural response of the cylinders are sensitive to the blast charge and core configuration. It is concluded that energy absorption capacity and maximum radial deflection are two conflicting goals for achieving high impact/blast resistance capability. An in-depth understanding of the behavior in sandwich-walled cylinders under blast impulse and the influence of the core configuration helps realize the advantages and disadvantages of using graded foam materials in sandwich structures and can provide a guideline for structural design.
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- 2020
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15. Dynamic Compressive Behaviors of Two-Layer Graded Aluminum Foams under Blast Loading
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Minzu Liang, Xiangyu Li, Yuliang Lin, Kefan Zhang, and Fangyun Lu
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aluminum foam ,gradient ,dynamic behavior ,energy absorption ,Voronoi technique ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Experimental and numerical analyses were carried out to reveal the behaviors of two-layer graded aluminum foam materials for their dynamic compaction under blast loading. Blast experiments were conducted to investigate the deformation and densification wave formation of two-layer graded foams with positive and negative gradients. The shape of the stress waveform changed during the propagation process, and the time of edge rising was extended. Finite element models of two-layer graded aluminum foam were developed using the periodic Voronoi technique. Numerical analysis was performed to simulate deformation, energy absorption, and transmitted impulse of the two-layer graded aluminum foams by the software ABAQUS/Explicit. The deformation patterns were presented to provide insights into the influences of the foam gradient on compaction wave mechanisms. Results showed that the densification wave occurred at the blast end and then gradually propagated to the distal end for the positive gradient; however, compaction waves simultaneously formed in both layers and propagated to the distal end in the same direction for the negative gradient. The energy absorption and impulse transfer were examined to capture the effect of the blast pressure and the material gradient. The greater the foam gradient, the more energy dissipated and the more impulse transmitted. The absorbed energy and transferred impulse are conflicting objectives for the blast resistance capability of aluminum foam materials with different gradient distributions. The results could help in understanding the performance and mechanisms of two-layer graded aluminum foam materials under blast loading and provide a guideline for effective design of energy-absorbing materials and structures.
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- 2019
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16. A Transfer-Learning-Like Neural Dynamics Algorithm for Arctic Sea Ice Extraction.
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Bo Peng, Kefan Zhang, Long Jin 0001, and Mingsheng Shang 0001
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- 2024
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17. Dynamic molecular tunnel junctions based on self-assembled monolayers for high tunneling current triboelectricity generation
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Lu Zhang, Awad Shalabny, Chen Su, Xin Cui, Amro Sweedan, Kefan Zhang, Sherina Harilal, Sumesh Sadhujan, and Muhammad Y. Bashouti
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Renewable Energy, Sustainability and the Environment ,General Materials Science ,General Chemistry - Abstract
Here, we show a novel energy conversion design with a dynamic MIM structure. This is realized though grafting CF3 group which shows high tunnelling current density of up to 7.6 × 106 Am−2. This can further fulfill the macroscopic energy harvesting.
- Published
- 2023
18. Upregulated miR-322-5p regulates cell cycle and promotes cell proliferation and apoptosis by directly targeting Wee1 in mice liver injury
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He Tong, Li Wang, Jing Shi, Haowei Jin, Kefan Zhang, Yulong Bao, Yongshuai Wu, Yipeng Cheng, Pengxia Liu, and Changshan Wang
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Cell Cycle ,Apoptosis ,Cell Biology ,Gene Expression Regulation, Neoplastic ,Mice ,MicroRNAs ,Chemical and Drug Induced Liver Injury, Chronic ,Cell Line, Tumor ,Animals ,Molecular Biology ,Carbon Tetrachloride ,Cell Division ,Developmental Biology ,Cell Proliferation - Abstract
Liver injury from any number of causes (e.g. chemical material, drugs and diet, viral infection) is a global health problem, and its mechanism is not clearly understood. MicroRNAs (miRNAs) expression profiling is gaining popularity because miRNAs, as key regulators in gene expression networks, can influence many biological processes and have also shown promise as biomarkers for disease. Previous studies reported the regulation effects of miRNAs in liver injury, whereas function and molecular mechanisms of miR-322-5p were still unclear. Therefore, our study focused on the biological role of miR-322-5p in carbon tetrachloride (CCl
- Published
- 2023
19. S100A6 Activates Kupffer Cells via the p-P38 and p-JNK Pathways to Induce Inflammation, Mononuclear/macrophage Infiltration Sterile Liver Injury in Mice
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He, Tong, Li, Wang, Kefan, Zhang, Jing, Shi, Yongshuai, Wu, Yulong, Bao, and Changshan, Wang
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Immunology ,Immunology and Allergy - Abstract
Noninfectious liver injury, including the effects of chemical material, drugs and diet, is a major cause of liver diseases worldwide. In chemical and drugs-induced liver injury, innate inflammatory responses are mediated by extracellular danger signals. The S100 protein can act as danger signals, which can promote the migration and chemotaxis of immune cells, promote the release of various inflammatory cytokines, and regulate the body's inflammatory and immune responses. However, the role of S100A6 in inflammatory response in chemical and drugs-induced sterile liver injury remains unclear. We constructed the model of sterile liver injury induced by carbon tetrachloride (CCl
- Published
- 2022
20. Recent progress on transition metal macrocycles for electrochemical CO2 reduction
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Kefan Zhang, Fang Lü, Na Han, and Yanguang Li
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Multidisciplinary - Published
- 2022
21. Study on the use of 3D printed guides in the individualized reconstruction of the anterior cruciate ligament
- Author
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Xin Wang, Dening Wang, Kefan Zhang, Changling Du, and Hui Shi
- Abstract
Objective Evaluation of the accuracy and effectiveness of 3D printed guides to assist femoral tunnel preparation in individualised reconstruction of the anterior cruciate ligament. Design Randomised controlled studies. Patients/participants Sixty patients who attended the Affiliated Hospital of Binzhou Medical College for autologous hamstring single bundle reconstruction of the anterior cruciate ligament from October 2018 to October 2020 were selected and randomly divided into two groups, including 31 cases in the 3D printing group (14 males and 17 females, mean age 41.94 ± 10.15 years) and 29 cases in the control group (13 males and 16 females, mean age 37.76 ± 10.34 years). Main outcome measures Patients in both groups were assessed for intraoperative femoral tunnel accuracy, the number of intraoperative positioning and the time taken to prepare the femoral tunnel, the length of the anteromedial approach incision, the pre-planned bone tunnel length and intraoperative bone tunnel length in the 3D printed group, IKDC score and Lysholm score preoperatively and at 3, 6 and 12 months postoperatively, the Lachman、pivot-shift test preoperatively and at 6 months postoperatively, gait analysis to assess internal and external rotation in flexion of the knee at 12 months postoperatively and postoperative complications in both groups. Results There was no statistical difference in functional knee scores and anteromedial approach incision length between the 3D printed and control groups (p > 0.05), while there was a statistical difference in the accuracy of tunnel positioning, the time taken to prepare the femoral bone tunnel and the degree of external rotation of the knee in flexion between the two groups (p 0.05). Complications: One case in the 3D printing group developed intermuscular vein thrombosis in the affected lower limb after surgery, which disappeared after treatment, while three cases in the control group developed intermuscular vein thrombosis in the affected lower limb. No complications such as bone tunnel rupture, deep vein thrombosis in the lower limb and infection occurred in either group. Conclusions 3D printed guides assisted with individualized ACL reconstruction can improve the accuracy of femoral tunnel positioning, which is safe and effective, while reducing the operative time and the number of intraoperative positioning, without increasing the length of incision, and can obtain higher functional scores and rotational stability of the knee joint, which is in line with the concept of individualized ACL reconstruction. Level of Evidence Therapeutic Level Ⅰ.
- Published
- 2023
22. Molecular Modulation of Sequestered Copper Sites for Efficient Electroreduction of Carbon Dioxide to Methane
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Kefan Zhang, Jie Xu, Tianran Yan, Lin Jia, Jie Zhang, Chaochen Shao, Liang Zhang, Na Han, and Yanguang Li
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Biomaterials ,Electrochemistry ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2023
23. Summer:A Small Modular Lead-Bismuth-Cooled Fast Reactor for Mobile Energy Supply
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Kefan Zhang, Weixiang Wang, Chengjie Duan, Xiangliang Chen, Wenshun Duan, Xiao Luo, and Hongli Chen
- Published
- 2023
24. A Moose-Based Neutron Diffusion Code with Application to a LMFR Benchmark
- Author
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Weixiang Wang, WenPei Feng, KeFan Zhang, Guangliang Yang, Tao Ding, and Hongli Chen
- Abstract
MOOSE (Multiphysics Object-Oriented Simulation Environment) is a powerful finite element multi-physics coupling framework, whose object-oriented, extensive system is conducive to the development of various simulation tools. In this work, a full-core MOOSE-based Neutron Diffusion application is developed, and a 3D PWR benchmark 3D-IAEA with given group constants is applied for code verification. Then the MOOSE-based Neutron Diffusion application is applied to the calculation of a Sodium-cooled Fast Reactor (SFR) benchmark, together with the research on homogenized few-group constants generation based on Monte Carlo method. The calculation adopts a 33-group cross section sets, which is generated using Monte Carlo code OpenMC. Considering the long neutron free path and strong global neutron spectrum coupling of liquid metal cooled reactor (LMFR), a full-core homogeneous model is used in OpenMC to generate the homogenized few-group constants. In addition, transport correction is used in the process of cross section generation, considering the prominent anisotropic scattering of fast reactor. The calculated results, including effective multiplication factor (keff) and assembly power distributions, are in good agreement with the reference values and the calculation results of OpenMC, which proves the accuracy of the neutron diffusion application, and also shows that the Monte Carlo method can be applied to generation of homogenized few-group constants for LMFR analysis.
- Published
- 2023
25. Valorizing carbon dioxide via electrochemical reduction on gas‐diffusion electrodes
- Author
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Yuhang Wang, Yuqing Luo, Kefan Zhang, and Yanguang Li
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Materials science ,carbon dioxide electroreduction ,reactors ,Information technology ,T58.5-58.64 ,Electrochemistry ,Reduction (complexity) ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Electrode ,Carbon dioxide ,TA401-492 ,Gaseous diffusion ,gas‐diffusion electrodes ,Materials of engineering and construction. Mechanics of materials - Abstract
The electrochemical carbon dioxide (CO2) reduction provides a means to upgrade CO2 into value‐added chemicals. When powered by renewable electricity, CO2 electroreduction holds the promise of chemical manufacturing with carbon neutrality. A commercially relevant CO2 electroreduction process should be highly selective and productive toward desired products, energetically efficient for power conversion, and stable for long‐term operation. To achieve these goals, designing gas‐diffusion catalytic electrodes and prototyping reactors built upon in‐depth understandings of the reaction mechanisms are of paramount importance. In this review, the fundamentals of gas‐diffusion electrodes are briefly presented. Then, the most recent advances in developing high‐performance CO2 reduction using gas‐diffusion electrodes are overviewed. Reactor engineering aiming at enhancing productivity, energy efficiency, CO2 single‐pass utilization, and operating lifetime is further discussed. Challenges in developing CO2 electroreduction systems are included. The prospects for advancing CO2 electroreduction toward practical applications are also narrated.
- Published
- 2021
26. Evaluation and Monitoring of the API Content of a Portable Near Infrared Instrument Combined with Chemometrics Based on Fluidized Bed Mixing Process
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Lanling Liu, Liang Zhong, Hengchang Zang, Hui Zhang, Ruiqi Huang, Lian Li, Haiyan Wang, Dongbo Xu, Kefan Zhang, and Wenping Yin
- Subjects
Mean squared error ,business.industry ,Near-infrared spectroscopy ,Mixing (process engineering) ,Pharmaceutical Science ,Cross-validation ,Chemometrics ,Fluidized bed ,Drug Discovery ,Content (measure theory) ,Partial least squares regression ,Environmental science ,Process engineering ,business - Abstract
With the promulgation of industry guidelines, near infrared spectroscopy (NIRS) monitoring the uniformity of solid oral preparations has become one of the most reported studies. The purpose of the present study is to develop a near infrared (NIR) method for the in-line assessment of the active pharmaceutical ingredient (API) content in the fluidized bed mixing process, as well as to introduce a portable NIR sensor to monitor the fluidized bed production visually. A portable NIR sensor was used to monitor the content of the API in the fluidized bed mixing process. An experimental fluidized bed was performed and the corresponding NIR spectra were collected in-line. The spectrum selection method of cosine distance for in-line spectra was performed, and four wavelength optimization methods were compared to improve the accuracy of the model. Throughout the mixing process, the content uniformity of the API was a critical quality attribute (CQA). The partial least squares regression (PLSR) quantitative model of API content was established. The results showed that the cosine distance combined with the multi-variable selection methods (CC, VIP, RATC, and UVE) could obtain more useful information than the single method. The root mean square error of cross validation (RMSECV) and root mean square error of prediction (RMSEP) values of the optimal PLSR model were 1.8588% and 1.5296%, respectively. Based on the results obtained, this study could be used as a reference for the pilot of in-line monitoring of miniature NIR sensors and the connection of key process parameters to realize intelligent production of solid preparations.
- Published
- 2021
27. Effect of packing structure on anisotropic effective thermal conductivity of thin ceramic pebble bed
- Author
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Shuai Wang, Hongli Chen, Shuang Wang, Bowen Wu, Kefan Zhang, and Yuelin Lu
- Subjects
Materials science ,020209 energy ,chemistry.chemical_element ,02 engineering and technology ,Blanket ,Discrete element method ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Breeder (animal) ,Thermal conductivity ,Three-dimensional thermal network method ,Vertical direction ,Pebble bed ,0202 electrical engineering, electronic engineering, information engineering ,Effective thermal conductivity ,Ceramic ,Composite material ,Pebble ,Anisotropy ,Helium ,TK9001-9401 ,Nuclear Energy and Engineering ,chemistry ,visual_art ,visual_art.visual_art_medium ,Nuclear engineering. Atomic power - Abstract
Helium cooled solid breeder blanket as an important blanket candidate of the Tokamak fusion reactor uses ceramic pebble bed for tritium breeding. Considering the poor effective thermal conductivity of the ceramic breeder pebble bed, thin structure of tritium breeder pebble bed is usually adopted in the blanket design. The container wall has a great influence on the thin pebble bed packing structure, especially for the assembly of mono-sized particles, and thin pebble bed will appear anisotropic effective thermal conductivity phenomenon. In this paper, thin ceramic pebble beds composed of 1 mm diameter L i 4 S i O 4 particles are generated by the EDEM 2.7. The effective thermal conductivity of different thickness pebble beds in the three-dimensional directions are analyzed by three-dimensional thermal network method. It is observed that thin L i 4 S i O 4 pebble bed showing anisotropic effective thermal conductivity under the practical design size. Normally, the effective thermal conductivity along the bed vertical direction is higher than the horizontal direction due to the gravity effect. As the thickness increases from 10 mm to 40 mm, the effective thermal conductivity of the pebble bed gradually increases.
- Published
- 2021
28. Optimal Design of Thermal Scheme for Liquid Metal Cooled High Flux Reactor Fuel Assembly
- Author
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Rui Pan, Kefan Zhang, Xilin Zhang, Jian Deng, Yong Zhang, Dahuan Zhu, and Hongli Chen
- Abstract
This paper studies the structural parameters of fuel assemblies suitable for high neutron flux density environments. Due to the high neutron flux density in the core, high-flux reactors provide an experimental environment for neutron irradiation. However, high neutron flux leads to high heat flux density on the fuel assembly surface, which brings challenges to the design of fuel assembly. Therefore, it is very important to study the structural design of fuel assemblies suitable for high neutron flux density environments. Through theoretical derivation of the thermal model of the fuel assembly and sensitivity analysis of the design parameters of the fuel assembly using a single-channel program, the results show that the liquid metal cooling plate fuel element can be well adapted to the high neutron flux Density environment; at relatively low neutron flux densities, bundle fuel elements can also meet reactor design requirements.
- Published
- 2022
29. Thermal-Hydraulic Safety Analysis of Natural Circulation Lead-Cooled Fast Reactor SNCLFR-100 Core Based on Porous Medium Approach
- Author
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Wenpei Feng, Guangliang Yang, Kefan Zhang, Chong Qin, Xiao Luo, Tao Ding, and Hongli Chen
- Abstract
In this study, a thermal-hydraulic analysis is carried out for the core of natural circulation lead-cooled fast reactor SNCLFR-100. Steady-state and transient analysis are performed with porous medium approach-based code TWOPORFLOW. In the steady-state analysis, mass flow distribution and temperature distributions of the assemblies are analyzed in assembly-wise mode. At the same time, the hottest assembly is analyzed in pin level and the safety performance is investigated. In the transient analysis, a typical Design Extension Conditions unprotected over-power transient is simulated with one-way coupling method.
- Published
- 2022
30. Fluid-Thermal-Mechanical Coupling Analysis of the Reactor Vessel of Natural Circulation Lead-Cooled Fast Reactor SNCLFR-100
- Author
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Wenshun Duan, Kefan Zhang, and Hongli Chen
- Abstract
Lead-based reactors mostly adopt integrated pool structures to simplify the design of reactor and have high inherent safety. As an important safety facility in the primary circuit, the reliability of the reactor vessel is critical for the safe operation of the whole reactor. The reactor vessel of natural circulation lead-cooled fast reactor SNCLFR-100 was taken as the object, and the corresponding simplified model was established. Based on ANSYS, the fluid-thermal-mechanical coupling analysis was carried out under the steady-state to study the influence of flow field and temperature field on the reactor vessel, and to reveal the distribution law of key thermal and mechanical parameters under the steady-state operation in this paper. The results of mechanical calculations were evaluated based on American society of Mechanical Engineers (ASME) standards, and the preliminary design scheme of reactor solid structure was optimized. The results showed that the stress evaluation results of the SNCLFR-100 reactor vessel meet the requirements of ASME standards in steady-state operation conditions. After optimizing the original solid structure, the stress evaluation results show that the maximum stress value was reduced by 43.3%, and the stress concentration was obviously suppressed, which indicates that the reactor container has a higher safety margin under the optimization scheme. The Steady-state calculation based on normal operation conditions provides a certain research basis for the subsequent transient calculation under accident conditions.
- Published
- 2022
31. The Thermal-Hydraulic Analysis and Optimization of Annular Fuel for Lead-Cooled Fast Reactor
- Author
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Kefan, Zhang, additional, Chong, Qin, additional, Wenmeng, Dong, additional, Rui, Pan, additional, Wenshun, Duan, additional, and Hongli, Chen, additional
- Published
- 2022
- Full Text
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32. Conceptual design of oil field energy supply system based on natural circulation lead–bismuth fast reactor
- Author
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Wenshun Duan, Shaoning Shen, Aoguang Wu, Kangli Shi, Kefan Zhang, Weixiang Wang, Xiao Luo, Zhao Chen, Hongli Chen, Daming Chen, and Yixuan Guo
- Subjects
Nuclear and High Energy Physics ,Nuclear Energy and Engineering ,Mechanical Engineering ,General Materials Science ,Safety, Risk, Reliability and Quality ,Waste Management and Disposal - Published
- 2023
33. Benefit Finding and Related Factors of Patients with Early-Stage Cancer in China
- Author
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Xichenhui, Qiu, Kefan, Zhang, Yan, Zhang, and Liyuan, Sun
- Subjects
China ,Cross-Sectional Studies ,Neoplasms ,Health, Toxicology and Mutagenesis ,benefit finding ,early-stage cancer ,social support ,coping mode ,Adaptation, Psychological ,Public Health, Environmental and Occupational Health ,Humans ,Social Support - Abstract
(1) Background: Although the research on benefit finding (BF) in China has increased in recent years, it remains in its infancy. Few previous studies have focused on early-stage cancer patients. Therefore, this research study aimed to explore BF and its influencing factors for early-stage cancer patients in China. (2) Methods: From April to August 2019, 319 patients with early-stage cancer in the treatment period were selected by the convenience sampling method and evaluated using the Benefit Finding of Cancer Patients Scale-Chinese (BFS-C), Perceived Social Support Scale (PSSS), and Medical Coping Modes Questionnaire (MCMQ). (3) Results: The mean BF score was 47.57 (SD = 12.26). The results of the correlation analysis show that benefit finding was positively correlated with social support, but negatively correlated with acceptance-resignation. In addition, social support was negatively correlated with avoidance and acceptance-resignation. The results of the multiple linear regression indicate that the variables of self-assessment of disease severity, exercise time, coping mode (acceptance-resignation), and social support, affect BF. Finally, social support was shown to exert an intermediary effect on acceptance-resignation and BF. (4) Conclusions: In this study, the score of BF of patients with early-stage cancer was low. Medical staff should be more aware of the health behavior of patients with early-stage cancer, guide them to actively face the disease, and fully mobilize the social support of patients’ friends and family, so as to help patients increase their disease BF.
- Published
- 2022
- Full Text
- View/download PDF
34. Correction to: S100A6 Activates Kupffer Cells via the p-P38 and p-JNK Pathways to Induce Inflammation, Mononuclear/Macrophage Infiltration Sterile Liver Injury in Mice
- Author
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He Tong, Li Wang, Kefan Zhang, Jing Shi, Yongshuai Wu, Yulong Bao, and Changshan Wang
- Subjects
Immunology ,Immunology and Allergy - Published
- 2023
35. S100A6 Promotes Inflammation and Infiltration of Mononuclear/Macrophages via the p-P38 and p-JNK Pathways in Acute Liver Injury
- Author
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He Tong, Li Wang, Kefan Zhang, Jing Shi, yongshuai Wu, Yulong Bao, and changshan wang
- Abstract
BackgroundThe phagocytic S100 protein, which mediates inflammatory responses and recruits inflammatory cells to sites of tissue damage, has long been known to be expressed in cells of myeloid origin. S100A6 belongs to the A group of the S100 protein family of Ca2+-binding proteins. Currently, the mechanism by which S100A6 mediates the inflammatory response and recruits inflammatory cells to the tissue injury site is unknown.MethodsA mouse model of carbon tetrachloride (CCl4)-induced acute liver injury (ALI) was established, and the transcriptomes of postinjury 2d and 5d liver tissues were sequenced. Enzyme-linked immunosorbent assay was used to determine the expression of inflammatory factors (TNF-α, IL-1β, IL-6, and IL-8) in the supernatant of the liver. Immunohistochemical analysis confirmed the expression of S100A6 in the liver cells. In vitro experiments proved the pro-inflammatory function of S100A6, and western blotting (WB) showed that the pathways were activated. The transwell experiment showed the infiltration of mononuclear/macrophages.ResultsWe found that S100A6 is highly expressed in liver cells during the most severe period of ALI, suggesting that it acts as an endogenous danger signal and has a pro-inflammatory function. In vitro, the mouse S100A6 recombinant protein was used to stimulate liver Kupffer cells to promote the secretion of TNF-α, IL-1β, IL-6, and IL-8. Further mechanistic experiments revealed that S100A6 acts as an endogenous danger signal to activate p-P38 and p-JNK downstream of the TLR4 and P65 pathways. Similarly, transcriptome data showed that S100A6 can activate the inflammatory response in Kuffer cells. WB revealed that S100A6 had no significant effect on cell apoptosis. To continue to explore the mechanism of monocyte/macrophage infiltration, we found that TNF-α stimulates liver cells as the main source of CCL2. TNF-α can initiate the p-P38 and p-JNK pathways of liver cells to produce CCL2, thereby recruiting the infiltration of mononuclear/macrophages. ConclusionsTaken together, S100A6 is an endogenous danger signal that mediates inflammatory responses and recruits inflammatory cells to sites of tissue damage.
- Published
- 2021
36. ESM and Detection Cooperated Jamming Strategy
- Author
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Hengwei Li, Yi Han, Kefan Zhang, and Tianxian Zhang
- Published
- 2021
37. Assessment of different internal flow blockage scenarios for LFR fuel assemblies with different configurations
- Author
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Xiao Luo, Kefan Zhang, Wenshun Duan, and Hongli Chen
- Subjects
Nuclear Energy and Engineering - Published
- 2022
38. The effect of relative humidity on vapor dispersion of liquefied natural gas: A CFD simulation using three phase change models
- Author
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Kefan Zhang, Xuanyi Zhou, and Beihua Cong
- Subjects
Renewable Energy, Sustainability and the Environment ,Mechanical Engineering ,Civil and Structural Engineering - Published
- 2022
39. LncRNA SNHG8 Promotes Liver Cancer Proliferation and Metastasis by Sponging miR-542-3p and miR-4701-5p
- Author
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Kefan Zhang, Yipeng Cheng, Li Wang, Jing Shi, qirile Sa, changshan wang, Pengxia Liu, He Tong, Haowei Jin, Shuang He, and Kexin Li
- Subjects
business.industry ,Cancer research ,Medicine ,Mir 542 3p ,business ,medicine.disease ,Liver cancer ,Metastasis - Abstract
Growing evidence suggests that long non-coding RNAs (lncRNAs) are associated with carcinogenesis and could function as competing endogenous RNAs (ceRNAs) to regulate microRNAs (miRNAs). LncRNA small nucleolar RNA host gene 8 (LncRNA SNHG8) is up-regulated in various cancers and positively associated with poor prognosis of these cancers. However, the molecular mechanisms by which lncRNA SNHG8 contributes to hepatocellular carcinoma (HCC) still remains unclear. In the present study, we reported that lncRNA SNHG8 was abnormally up-regulated in liver cancer tissues and HCC cell lines. Moreover, knockdown of lncRNA SNHG8 significantly attenuated the proliferation, migration, invasion process of HCC cell line HepG2 in vitro. Mechanistically, we first reported that suppression of lncRNA SNHG8 evidently enhanced miR-542-3p and miR-4701-5p expression and decreased TET3 expression at posttranscriptional level. Furthermore, lncRNA SNHG8 upregulated TET3 expression by sponging miR-542-3p and miR-4701-5p by competing binding. Taken together, our results confirmed the oncogene role of lncRNA SNHG8 and discovered the underlying mechanism that lncRNA SNHG8 upregulated TET3 through the sponging or decaying of miR-542-3p and miR-4701-5p in human hepatocellular carcinoma, suggesting that lncRNA SNHG8 may serve as a potential diagnostic marker and therapeutic target for patients with hepatocellular carcinoma.
- Published
- 2021
40. Study on dynamic response of gravity dam under air blast load based on similarity law
- Author
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Kefan Zhang, Fangyun Lu, Yong Peng, and Xiangyu Li
- Subjects
General Engineering ,General Materials Science - Published
- 2022
41. Traditional Mongolian medicine (HHQG) attenuates CCl4-induced acute liver injury through inhibiting monocyte/macrophage infiltration via the p-P38/p-JNK pathway
- Author
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Li Wang, Yulong Bao, He Tong, Kefan Zhang, Yipeng Cheng, Haowei Jin, Jing Shi, Tegexibaiyin Wang, Haisheng Wang, Guilin Chen, and Changshan Wang
- Subjects
Pharmacology ,Drug Discovery - Published
- 2022
42. The PERK-EIF2α-ATF4 signaling branch regulates osteoblast differentiation and proliferation by PTH
- Author
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Yingjiang Li, Ninghan Feng, Yu Wu, Chunping Li, Xiuxia Qu, Miaomiao Wang, Shaidi Tang, and Kefan Zhang
- Subjects
0301 basic medicine ,Physiology ,Endocrinology, Diabetes and Metabolism ,Eukaryotic Initiation Factor-2 ,Osteoporosis ,Parathyroid hormone ,Core Binding Factor Alpha 1 Subunit ,Peptide ,Mice ,eIF-2 Kinase ,0302 clinical medicine ,Glycine Plasma Membrane Transport Proteins ,Benzoquinones ,Enzyme Inhibitors ,media_common ,chemistry.chemical_classification ,biology ,Cell Differentiation ,Osteoblast ,Endoplasmic Reticulum Stress ,Hsp90 ,medicine.anatomical_structure ,Parathyroid Hormone ,030220 oncology & carcinogenesis ,Signal Transduction ,Drug ,endocrine system ,medicine.medical_specialty ,Lactams, Macrocyclic ,media_common.quotation_subject ,Osteocalcin ,Collagen Type I ,Cell Line ,03 medical and health sciences ,Cyclin D ,Physiology (medical) ,Internal medicine ,CDC2 Protein Kinase ,Cyclin E ,medicine ,Animals ,HSP90 Heat-Shock Proteins ,Cell Proliferation ,Osteoblasts ,business.industry ,Cell growth ,ATF4 ,Alkaline Phosphatase ,medicine.disease ,Activating Transcription Factor 4 ,Collagen Type I, alpha 1 Chain ,030104 developmental biology ,Endocrinology ,chemistry ,biology.protein ,business - Abstract
Parathyroid hormone (PTH) and its related peptide (PTH-related peptide 1–34) are two of the Food and Drug Administration-approved bone-promoting drugs for age-related osteoporosis. Treatment with PTH stimulates bone formation. However, the molecular mechanisms of PTH-mediated osteoblast differentiation and cell proliferation are still not completely understood. In this study, we showed that PTH induced endoplasmic reticulum (ER) stress in osteoblasts through the PKR-like endoplasmic reticulum kinase (PERK)-eukaryotic initiation factor 2α (EIF2α)-activating transcription factor 4 (ATF4)-signaling pathway. After separately blocking PERK-EIF2α-ATF4 signaling with two different inhibitors [AMG’44 and integrated stress response inhibitor (ISRIB)] or specific small interfering RNA for PERK and ATF4, the following targets were all downregulated: expression of osteoblast differentiation markers [runt-related transcription factor 2 (Runx2), alkaline phosphatase (Alp), type I collagen (Col1a1), and osteocalcin (Ocn)], cell proliferation markers (CyclinE, CyclinD, and CDC2), amino acid import (Glyt1), and metabolism-related genes (Asns). Additionally, Alp-positive staining cells, Alp activity, matrix mineralization, Ocn secretion, and cell proliferation indexes were inhibited. Interestingly, we found that salubrinal enhanced PTH-induced osteoblast differentiation and proliferation by maintenance of phosphorylation of EIF2α. Furthermore, we observed that PTH increased the association between heat shock protein 90 (HSP90) and PERK and maintained PERK protein stabilization in the early stages of PTH-induced ER stress. Treatment of MC3T3-E1 cells with geldanamycin, an HSP90 inhibitor, decreased PERK protein expression and inhibited osteoblast differentiation and cell proliferation upon PTH treatment. Taken together, our data demonstrate that PTH regulates osteoblast differentiation and cell proliferation, partly by activating the HSP90-dependent PERK-EIF2α-ATF4 signaling pathway.
- Published
- 2019
43. Method development and validation of a near-infrared spectroscopic method for in-line API quantification during fluidized bed granulation
- Author
-
Liang Zhong, Lele Gao, Lian Li, Lei Nei, Yongheng Wei, Kefan Zhang, Hui Zhang, Wenping Yin, Dongbo Xu, and Hengchang Zang
- Subjects
Principal Component Analysis ,Spectroscopy, Near-Infrared ,Least-Squares Analysis ,Instrumentation ,Chromatography, High Pressure Liquid ,Spectroscopy ,Atomic and Molecular Physics, and Optics ,Analytical Chemistry - Abstract
Near-infrared spectroscopy (NIRS) is an excellent process analytical technology (PAT) tool for active pharmaceutical ingredient (API) quantification during fluidized granulation. Therefore, a portable near-infrared spectrometer combined with a new innovative method of extended iterative optimization technique (EIOT) was used to in-line monitor the API content uniformity during fluidized bed granulation. The principal component analysis (PCA) and partial least squares regression (PLSR) were also used to characterize and predict API concentration with changes from 75% to 125% of the label claim to prove the superiority of EIOT. The API content prediction accuracy of the EIOT method was verified through offline High Performance Liquid Chromatography (HPLC) measurement. Also, the spatial distribution of API in granules was visualized by Raman imaging technology. The results showed that the established NIRS method was suitable for the prediction of API content in fluidized bed granulation, which provides a new idea for the determination of API content during granulation.
- Published
- 2022
44. Design Methods for the Lethality of Fragmentation Warhead based on a Multi-Objective Optimization Algorithm
- Author
-
Xiangyu Li, Fugang Li, Minzu Liang, and Kefan Zhang
- Subjects
History ,Computer Science Applications ,Education - Abstract
Fragmentation warhead is the main weapon used to damage personnel and light armored objectives, with the lethal parameters primarily consisting of the number and velocity of fragments. Increasing the lethality of fragmentation warhead is currently the most important development direction for fragmentation warhead. The formation process of natural fragment is complicated and there are several factors that affect the properties of fragment, so there are still many techniques of developing the lethality of fragmentation warhead. In this paper, our object is to focus on the optimization design for the lethality of fragmentation warhead, based on the optimal Latin hypercube test design method and the radial basis function neural network surrogate modelling, taking the number of effective fragments per unit mass and the average kinetic energy of the effective fragments as the optimization objectives, and the explosive charge, the fragment material, the overall structure and initiation method are considered, respectively. The Pareto optimal solution sets under different design variables are obtained, and the optimal matching of multiple design parameters is realized, establishing the integration design method for “explosive - material-structure” of the fragmentation warhead. The research results can provide technical support for the structural optimization design of the fragmentation warhead.
- Published
- 2022
45. Mechanics of Plate Fracture from Detonation Wave Interaction
- Author
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Kefan Zhang, Minzu Liang, Fangyun Lu, and Xiangyu Li
- Subjects
Materials science ,Explosive material ,General Chemical Engineering ,Detonation ,Plate fracture ,02 engineering and technology ,General Chemistry ,Mechanics ,Collision ,01 natural sciences ,010305 fluids & plasmas ,020303 mechanical engineering & transports ,0203 mechanical engineering ,0103 physical sciences - Published
- 2018
46. Research on Dynamic Constitutive Model and Fracture Characteristics of Two High Strength Steels
- Author
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Xiangyu Li, Fugang Li, Minzu Liang, Kefan Zhang, and Zhandong Tian
- Subjects
History ,Computer Science Applications ,Education - Abstract
58SiMn and 50SiMnVB are commonly used shell materials in bombs, and their dynamic constitutive model and fracture properties directly determine the mass distribution of the bomb. Tensile tests were carried out on two high-strength steels, and the parameters of the yield point, tensile strength and plastic failure strain as well as the John-Cook model were determined. The fracture morphologies of the two steels in the quasi-static tension and in the dynamic tension were analyzed. The research results show that the yield strength of 58SiMn steel remains unchanged with increasing strain rate. The yield strength and tensile strength of 50SiMnVB increase as the rate of elongation increases, and the ability to plastically deform decreases, which shows the properties of embrittlement under high speed loading. With the same elongation rate, 50SiMnVB steel has higher strength and toughness. 58SiMn steel is a tensile brittle fracture from a macroscopic point of view and a quasi-split fracture from a microscopic point of view; 50SiMnVB steel is a mixed lap shear fracture under axial tensile load. With increasing loading rate, the tensile fracture tends to pure shear fracture.
- Published
- 2022
47. A new perspective in understanding the dissolution behavior of nifedipine controlled release tablets by NIR spectroscopy with aquaphotomics
- Author
-
Qin Dong, Lanling Liu, Zhongyu Sun, Kefan Zhang, Lian Li, and Hengchang Zang
- Subjects
Aqueous solution ,Chemistry ,In vitro dissolution ,Organic Chemistry ,Near-infrared spectroscopy ,Controlled release ,Analytical Chemistry ,Inorganic Chemistry ,Chemical engineering ,Scientific method ,medicine ,Swelling ,medicine.symptom ,Layer (electronics) ,Dissolution ,Spectroscopy - Abstract
The in vitro dissolution profile is a key parameter which reflects the quality of sustained-release tablets. Most studies were used to determine the dissolution of tablets utilizing dissolution curves. This study aimed to investigate the role of water during nifedipine controlled release tablets dissolution process in order to provide a new way to understand the dissolution behavior. Dissolution liquid was added to the drug-containing layer slowly, and near infrared spectra were acquired according to the set time. NIR spectroscopy with aquaphotomics were introduced to determine different water patterns. The results showed that, the structural change of water was consistent with the dissolution process. During hydration stage, it was mainly Sr combined with the semi-permeable membrane to form a channel. Then S0, S1, S2 and S3 combined with the drug layer and the push layer. Finally, it was relied on the swelling of the push layer to push the drug layer out at the release process. This study not only deepens the understanding of the dissolution process, but also opens a new perspective in molecular interaction analysis in aqueous system by understanding the roles of water.
- Published
- 2021
48. Effect of packing structure on mechanical properties of the pebble beds and the probability of particle crushing
- Author
-
Shuang Wang, Kefan Zhang, Hongli Chen, Manman Xu, and Shuai Wang
- Subjects
Mechanical load ,Materials science ,Mechanical Engineering ,chemistry.chemical_element ,Fusion power ,Blanket ,01 natural sciences ,010305 fluids & plasmas ,Breeder (animal) ,Nuclear Energy and Engineering ,chemistry ,visual_art ,0103 physical sciences ,visual_art.visual_art_medium ,Particle ,General Materials Science ,Ceramic ,Composite material ,010306 general physics ,Pebble ,Helium ,Civil and Structural Engineering - Abstract
The tritium ceramic breeder pebble bed, used for tritium breeding and in nuclear fusion reactor, is a key constituent of helium cooled solid breeder blankets. The mechanical properties of the pebble beds, along with the integrity of the particles are very important for the extraction of tritium and safe operation of the solid blanket. In this paper, analysis is carried out on the effect of packing structure, with varying bed thicknesses and particle smoothness, on mechanical properties and the probability of particle crushing. Pebble beds are randomly packed by a single type of Li 4 SiO 4 particle, 1 mm in diameter. It is found that when the thickness of the Li 4 SiO 4 pebble bed (ranging from 15 mm to 25 mm) becomes thinner, or the coefficient of friction (ranging from 0.1 to 0.6) increases, the strain of the pebble bed and the probability of particle crushing increases under the same mechanical load. Pebble beds with different packing structures have the same probability distribution function of normalized contact forces. The results could serve to characterize the tritium pebble beds and to optimize the design of helium cooled solid breeder blankets.
- Published
- 2021
49. Multiobjective blast-resistance optimization of gradient foam sandwiched cylindrical container
- Author
-
Xiangyu Li, Minzu Liang, Yuliang Lin, Kefan Zhang, and Fangyun Lu
- Subjects
Materials science ,Optimization problem ,business.industry ,Mechanical Engineering ,020101 civil engineering ,02 engineering and technology ,Building and Construction ,Structural engineering ,Multi-objective optimization ,0201 civil engineering ,Face sheet ,020303 mechanical engineering & transports ,0203 mechanical engineering ,Performance ratio ,Energy absorption ,Deflection (engineering) ,Specific energy absorption ,Crashworthiness ,business ,Civil and Structural Engineering - Abstract
The design and multiobjective optimization of graded foam-cored sandwich cylinders (GFSCs) composed of tubular face sheets and layered gradient foam core under internal air-blast loading were examined. The response surface method and nondominated sorting genetic algorithm were used in the defined optimization problem to determine the optimal face-sheet thickness and core gradient as well as achieve the minimum specific deflection of outer face sheet (SDF) and maximum specific energy absorption (SEA). Multiobjective optimization was subsequently conducted to obtain the Pareto front and improve the blast-resistant performance of the optimum GFSC configuration. Results obtained via these optimizations further indicated that the optimal GFSCs demonstrated better energy absorption characteristics than ordinary sandwich cylinders. Under the same SDF, SEA increased by 75.4% and 45.0% compared with that of the initial baseline design when the core gradient and face-sheet thickness were individually optimized, respectively. The joint optimization of face-sheet thicknesses and core gradient could significantly improve the blast-resistant performance by 171.1%. The optimization of GFSCs should primarily optimize the design variables with high-price performance ratio while reasonably arranging the design variable quantity, ensuring the controllable cost of calculation, and significantly improving the blast-resistant performance. The results also showed that the strength of CFSCs and the interaction between the face sheets and the core improved the blast-resistant capacity. The design optimization results could provide appropriate guidance in the crashworthiness design of GFSCs.
- Published
- 2020
50. Disulfiram chelated with copper promotes apoptosis in human breast cancer cells by impairing the mitochondria functions
- Author
-
Lixin Chen, Kefan Zhang, Xiaoxue Sun, Haifeng Yang, Yawei Wang, Zhihong Liang, Linyan Zhu, Yaping Yang, Mengjia Li, and Liwei Wang
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,Reactive oxygen species ,medicine.diagnostic_test ,Cell ,Mitochondrion ,Atomic and Molecular Physics, and Optics ,Flow cytometry ,Cell membrane ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,chemistry ,Biochemistry ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer cell ,medicine ,Biophysics ,Instrumentation ,Intracellular - Abstract
Summary Disulfiram (DSF) has been proved to have broad-spectrum anti-alcoholism effects, and it is also found to show stronger anti-tumor effects after chelating with Cu2+ to form DSF-Cu complex. In this work, we studied the anti-tumor activity of DSF-Cu in MCF-7 cells by flow cytometry, confocal laser scanning microscope, and atomic force microscopy to clarify the underlying anti-tumor mechanisms. MCF-7 cells were incubated with 50, 100, 150, 200, and 250 nM DSF chelated with 10 µM CuCl2 for 24 h. The results showed that DSF-Cu could induce the accumulation of MCF-7 cells in G2/M phase and apoptosis in a concentration-dependent manner. Additionally, atomic force microscope (AFM) analysis at nanoscale level showed that the morphology of cell was significantly shrunk with destroyed filopodia and ultrastructure presented many irregular protuberances on the cell membrane after DSF-Cu treatment, which was closely associated with the re-arrangement of cytoskeleton. DSF-Cu induced the production of reactive oxygen species (ROS), increased the concentration of intracellular Ca2+ and decreased the mitochondrial membrane potential (MMP) in MCF-7 cells resulting in a mitochondria-dependent apoptosis pathway. The results indicated that DSF-Cu has a potential anti-tumor activity in breast cancer by impairing the mitochondria functions. SCANNING 9999:1–12, 2016. © 2016 Wiley Periodicals, Inc.
- Published
- 2016
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