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Doubly re-entrant surfaces inspired by springtails exhibit excellent repellency to low-surface-tension liquid. However, the flexible doubly re-entrant surfaces are difficult to fabricate, especially for the overhang of the structure. Herein, we demonstrate a simple Fresnel aperture diffraction modulation strategy in microscale lithography coupled with a molding process to obtain the flexible doubly re-entrant superomniphobic surfaces with nanoscale overhangs. The negative nanoscale overhang features were formed in a single-layer photoresist due to the fine-modulation of the optical intensity fluctuation of the Fresnel aperture diffraction. The as-prepared flexible non-fluorinated polydimethylsiloxane (PDMS) doubly re-entrant microstructure based on the Fresnel aperture diffraction (D-BF) surface (without any additional treatments) could repel ethanol droplets (21.8 mN m

The Coronavirus disease 19 (COVID-19) pandemic has accumulated over 550 million confirmed cases and more than 6.34 million deaths worldwide. Although vaccinations has largely protected the population through the last two years, the effect of vaccination has been increasingly challenged by the emerging SARS-CoV-2 variants. Although several therapeutics including both monoclonal antibodies and small molecule drugs have been used clinically, high cost, viral escape mutations, and potential side effects have reduced their efficacy. There is an urgent need to develop a low cost treatment with wide-spectrum effect against the novel variants of SARS-CoV-2.Here we report a product of equine polyclonal antibodies that showed potential broad spectrum neutralization effect against the major variants of SARS-CoV-2. The equine polyclonal antibodies were generated by horse immunization with the receptor binding domain (RBD) of SARS-CoV-2 spike protein and purified from equine serum. A high binding affinity between the generated equine antibodies and the RBD was observed. Although designed against the RBD of the early wild type strain sequenced in 2020, the equine antibodies also showed a highly efficient neutralization capacity against the major variants of SARS-CoV-2, including the recent BA.2 Omicron variant (IC50 =1.867μg/ml) in viral neutralization assay in Vero E6 cells using live virus cultured. The broad-spectrum neutralization capacity of the equine antibodies was further confirmed using pseudovirus neutralization assay covering the major SARS-CoV-2 variants including wild type, alpha, beta, delta, and omicron, showing effective neutralization against all the tested strains. Ex vivo reconstructed human respiratory organoids representing nasal, bronchial, and lung epitheliums were employed to test the treatment efficacy of the equine antibodies. Antibody treatment protected the human nasal, bronchial, and lung epithelial organoids against infection of the novel SARS-CoV-2 variants challenging public health, the Delta and Omicron BA.2 isolates, by reducing >95% of the viral load. The equine antibodies were further tested for potential side effects in a mouse model by inhalation and no significant pathological feature was observed.Equine antibodies, as a mature medical product, have been widely applied in the treatment of infectious diseases for more than a century, which limits the potential side effects and are capable of large scale production at a low cost. A cost-effective, wide-spectrum equine antibody therapy effective against the major SARS-CoV-2 variants can contribute as an affordable therapy to cover a large portion of the world population, and thus potentially reduce the transmission and mutation of SARS-CoV-2.

To accurately assess the combustion fluctuation for a scramjet, a new method based on indium tin oxide (ITO) thin-film thermocouples (TFTCs) and time–frequency analysis (TFA) was developed in this study. ITO TFTCs were used to measure the transient wall-temperature change of the combustor, and the combustion fluctuation features were extracted from the temperature signal via TFA. The combustion fluctuation-resolving abilities of different TFA methods, including the short-time Fourier transform (STFT), continuous wavelet transform (CWT), and Hilbert–Huang transform (HHT), were investigated using a reconstruction signal. The results indicated that the CWT can provide an excellent global view and that the HHT is useful for localizing the frequency features in the time domain. Then, the effect of the equivalence ratio (ER) on the supersonic combustion fluctuation was researched. The total energy of the fluctuation was strongly correlated with the increase in the ER. Meanwhile, the frequency of main fluctuation was always concentrated at approximately 395 Hz, just like an intrinsic characteristic of the combustor. Additionally, the fuel type affected the energy of the combustion fluctuation, rather than the frequency.

Torreya grandis (Torreya grandis cv. Merrillii) is a unique nut tree species in China. Currently, researches on Torreya grandis focus on nuts quality and yield, while few works are related to the soil quality of Torreya grandis plantation. In this study, the typical Torreya grandis production areas of Zhuji, Shengzhou, Keqiao and Dongyang cities along the Kuaiji Mountain were selected. A total of 121 topsoil samples (0-20 cm) were collected based on a grid of 1 km × 1 km. The results indicated that the average concentrations of Cd, Cr, Cu, As, Ni and Pb in soils were 0.12, 49.01, 27.95, 14.28, 26.97 and 40.28 mg kg

Objective: Thyroglobulin antibodies (TgAb), principally comprising immunoglobulin G (IgG), are frequently found in healthy individuals. Previously, we showed that the glycosylation levels of TgAb IgG differed across various thyroid diseases, suggesting an important role of glycosylation on antibodies in the pathogenesis of thyroid diseases. Since IgG1 and IgG4 are the primary TgAb IgG subclasses, this study aimed to investigate the glycosylation of TgAb IgG1 and IgG4 subclasses in thyroid diseases. Methods: TgAb IgG was purified by affinity chromatography from the serum of patients with Hashimoto’s thyroiditis (HT) (n = 16), Graves’ disease (GD) (n = 8), papillary thyroid carcinoma (PTC) (n = 6), and PTC with histological lymphocytic thyroiditis (PTC-T) (n = 9) as well as healthy donors (n = 10). TgAb IgG1 and IgG4 concentrations were determined by enzyme-linked immunosorbent assay, and a lectin microassay was used to assess TgAb IgG1 and IgG4 glycosylation. Results: Significantly elevated mannose, sialic acid, and galactose levels on TgAb IgG1 were found in HT and PTC patients compared to GD patients and healthy controls (all p < 0.05). The mannose, sialic acid, and core fucose levels on TgAb IgG1 in PTC-T patients were higher than in healthy controls (all p < 0.05). Additionally, TgAb IgG1 from PTC-T patients exhibited lower sialylation than that from patients with PTC and higher fucosylation than that from patients with HT (both p < 0.05). However, TgAb IgG4 glycosylation did not differ among the five groups (p < 0.05). Conclusion: Our study describes different distributions of TgAb IgG1 glycosylation in various thyroid diseases. The aberrantly increased glycosylation levels of TgAb IgG1 observed in HT, PTC, and PTC-T might be indicative of immune disorders and participate in the pathogenesis of these diseases.

The effect of annealing on the thermoelectricity of indium tin oxide (ITO) thin film thermocouples (TFTCs) was studied. A microstructure characterisation indicate the influence of two different annealing mechanisms on the thermoelectric properties, namely, recrystallisation and carrier concentration. The ITO thin films recrystallised when the annealing temperature reached 1100 ∘ C in air, which improved the thermoelectric reproducibility, linearity, and stability of the TFTCs. More oxygen vacancies were produced in the ITO thin films after annealing at 400 ∘ C in vacuum, but were reduced after annealing at 1100 ∘ C in air. The Sn2+ ions transformed into Sn4+ ions in the ITO 90/10 thin films after annealing, providing additional free electrons in the films. These microstructural transformations are related to changes in the thermoelectric properties of the ITO TFTCs, as described by the thermoelectric theory of degenerated semiconductors. A decrease in the Seebeck coefficient after annealing was attributed to increases in the carrier concentrations and recrystallisation. Additionally, after annealing at 1100 ∘ C in air, the Seebeck coefficient of the ITO TFTCs agreed with the theoretical value. These results can be used to control thin film thermoelectric properties by different annealing processes.

The wall shear stress and pressure are important for analyzing boundary layer flow and evaluating the aerodynamic performance of the aircraft. In this study, a flexible skin consisting of dual layer hot-film sensors and pressure belts was developed to measure the distribution of wall shear stress and pressure on an unmanned aerial vehicle during increase of angle of attack (AOA), tail spin, and decreases of AOA. The sensitivity of the dual layer hot-film sensor is improved by about 150% due to heat conduction reduced. The relative error of pressure belt is less than 1% at 10 °C–65 °C. The boundary layer separation and reattachment time, separation AOA and time-shift of the flow field changes in flight conditions are determined. The separated AOA of the left and right wing boundary layers are 26.41° and 17.58° respectively. There is a delay about 4 s between the separation of the boundary layer and the entry of the tail spin, which can provide early warning to prevent abnormal flight conditions such as post stall and tail spin.

Antibody-based drugs can be highly toxic, because they target normal tissue as well as tumor tissue. The pH value of the extracellular microenvironments around tumor tissues is lower than that of normal tissues. Therefore, antibodies that engage in pH-dependent binding at slightly acidic pH are crucial for improving the safety of antibody-based drugs. Thus, we implemented a stepwise mutagenesis approach to engineering pH-dependent antibodies capable of selective binding in the acidic microenvironment in this study. The first step involved single-residue histidine scanning mutagenesis of the antibody's complementarity-determining regions to prescreen for pH-dependent mutants and identify ionizable sensitive hot-spot residues that could be substituted by acidic amino acids to obtain pH-dependent antibodies. The second step involved single-acidic amino acid residue substitutions of the identified residues and the assessment of pH-dependent binding. We identified six ionizable sensitive hot-spot residues using single-histidine scanning mutagenesis. Nine pH-dependent antibodies were isolated using single-acidic amino acid residue mutagenesis at the six hot-spot residue positions. Relative to wild-type anti-CEA chimera antibody, the binding selectivity of the best performing mutant was improved by approximately 32-fold according to ELISA and by tenfold according to FACS assay. The mutant had a high affinity in the pH range of 5.5-6.0. This study supports the development of pH-dependent protein switches and increases our understanding of the role of ionizable residues in protein interfaces. The stepwise mutagenesis approach is rapid, general, and robust and is expected to produce pH-sensitive protein affinity reagents for various applications.

Mesenchymal stem cells (MSCs) are considered to be critical for regenerative medicine. However, long-term culturing of MSCs will induce aging of MSCs, and thereafter impair cellular function. Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important. In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days. Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry. We identified 66 proteins (fold change1.50) that were differentially expressed in long-term culture. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification. Glycosylation analysis indicates that cell N-glycan patterns are associated with aging of MSCs. Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.

Nitrogen (N) fertilization is known to have a substantial effect on heavy metal uptake in plants. However, the impact of N fertilization on plant growth and heavy metal uptake of hyperaccumulator plants remains unclear. This study examined the effect of N fertilization on growth and uptake of zinc (Zn) and cadmium (Cd) in Zn/Cd-hyperaccumulating plants species, Sedum alfredii (S. alfredii) Hance (Crassulaceae). Plants of S. alfredii were grown for 60 days in nutrient solution with 100 μmol Cd L−1 and 0–10 mmol N L−1, and in a Cd-contaminated soil receiving N fertilizer and composted pig manure amendment. Biomass production, nutrient uptake, and concentrations and accumulation of Zn and Cd in plant parts were measured. In the hydroponic experiment, both low ( 5 mmol L−1) N supply decreased the growth and Zn and Cd accumulation in the whole plants. The 2.5 mmol N L−1 is an optimal N dosage for shoot biomass production and Zn accumulation in shoots, while the 1.0 mmol N L−1 is an optimal N dosage for Cd accumulation in shoots, which was 68.1% higher than the control. The N doses of 1 to 2.5 mmol L−1 N represented optimal conditions for Zn and Cd accumulation in the shoots of S. alfredii seedlings. In the soil pot experiment, shoot dry weight decreased with increasing N fertilization rate, while composted pig manure decreased biomass production. Both Zn and Cd accumulation in the shoots of S. alfredii decreased along with the addition of higher N fertilization rate. However, the composted pig manure amendment increased the accumulation of Zn, but not Cd in the shoots. The application of N at appropriate amount enhanced the phytoremediation efficiency by S. alfredii in Zn/Cd-polluted fields, but the effectiveness of phytoextraction technology needs to be validated in the field trials.

Environmental pollution caused by electrical waste (E-waste) dismantling has attracted much attention from environmental researchers in China and worldwide. A total of 90 topsoil samples, 10 soil profile samples and 14 groundwater samples, were collected from a typical E-waste dismantling area in southeastern China. The spatial and vertical variations of potentially hazardous metals (PHM) in paddy soils and their potentially ecological risks were studied. The results showed that the mean total concentrations of five PHM (Cd, Cu, Pb, Zn and Ni) in paddy soils were 0.38, 35.13, 35.40, 121.38 and 28.13 mg kg−1, respectively. The total Cd, and Pb concentrations in paddy soils had strong variability coefficients of 121.05% and 109.38%, respectively. Compared with the background values in Zhejiang province, the PHM were enriched in paddy soils. Part of the study area was seriously contaminated by these metals. The soil was contaminated with Cd, with high Pi Ave value (>1). The shallow groundwater was also contaminated by Cd in the E-waste dismantling area. Results of vertical variations of PHM in soils revealed significant accumulation of PHM in the topsoil (0–20 cm, 20–40 cm). Soil pH and organic matter (SOM) was significantly correlated with most of the total PHM in soils. Kriging interpolation and Moran's I were used to identify the contaminated hotspots of these PHM. It was found that high soil Cd, Cu, Ni, Zn were located in Da Xi and Zhe Guo towns, which was attributed to E-waste dismantling and other anthropogenic activities. Soil Ni was mainly influenced by the parent material. The PHM in soils may pose a potential threat to local ecosystem and human health.

Monitoring the temporal and spatial variation of soil properties is helpful to understand the evolution of soil properties and adjust the management method in time. Soil fertility evaluation is an urgent need to understand soil fertility level and prevent soil degradation. Here, we conducted an intensive field investigation in Chinese hickory (Carya cathayensis Sarg.) plantation to clarify the spatial and temporal variation of soil properties and its influencing factors, and to evaluate the change of soil fertility. The results showed that the soil pH and soil organic carbon (SOC) significantly increased from 2008 to 2018, while available nitrogen (AN) significantly decreased from 2008 to 2018. The semi-variance revealed that except available phosphorus (AP), the spatial dependencies of soil properties increased from 2008 to 2018. An increasing south-north gradient was found for soil AN, AP, available potassium (AK) and SOC and a decreasing south-north gradient was found for soil pH. The average soil fertility in the whole area was increased from 2008 to 2018. Our findings demonstrated that the changes of the management measures were the reason for the change of soil properties from 2008 to 2018. Therefore, rational fertilization strategies and sod cultivation are recommended to maintain the long-term development of the producing forest.

BackgroundChanges in IgG glycosylation, as a novel pathological feature, are observed in various autoimmune diseases (AIDs). The glycosylation patterns of IgG play a critical role in regulating the biological function and stability of IgG involved in the pathophysiology of many AIDs. However, the intracellular regulatory mechanisms underlying the effects of disturbances in various cytokines on IgG glycosylation are poorly understood. Thus, we investigated the regulatory effects of elevated cytokines in AIDs on intracellular IgG glycosylation within B cells.MethodsFirst, we established a controlled primary culture system in vitro to differentiate human CD19+ B cells into antibody-secreting cells (ASCs). Then, the IgG concentrations in the supernatants were measured by enzyme-linked immunoassay (ELISA) under IFN-γ, TNF-α, IL-21, IL-17A, BAFF, or APRIL stimulation. Next, the glycosylation levels of IgG under different stimuli were compared via a lectin microarray. The fine carbohydrate structures of IgG were confirmed by matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight-mass spectrometry (MALDI-TOF-MS). Finally, the expression of glycosyltransferases and glycosidases in B cells under stimulation with several cytokines was detected by real-time PCR and western blotting.ResultsWe found that cytokines significantly promoted IgG production in vitro and led to considerably different IgG glycan patterns. Specifically, the results of lectin microarray showed the galactose level of IgG was increased by IFN-γ stimulation (ppConclusionAbnormally elevated cytokines in the microenvironment regulates IgG glycan patterns by regulating intracellular glycosyltransferases in human B cells.

The densely glycosylated spike (S) protein highly exposed on severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) surface mediates host cell entry by binding to the receptor angiotensin-converting enzyme 2 (ACE2). However, the role of glycosylation has not been fully understood. In the present study, we investigated the effect of different N-glycosylation of S1 protein on its binding to ACE2. Using real-time surface plasmon resonance assay the negative effects were demonstrated by the considerable increase of binding affinities of de-N-glycosylated S1 proteins produced from three different expression systems including baculovirus-insect, Chinese hamster ovarian and two variants of human embryonic kidney 293 cells. Molecular dynamic simulations of the S1 protein-ACE2 receptor complex revealed the steric hinderance and Coulombic repulsion effects of the different types of N-glycans on the S1 protein interaction with ACE2. The results presented here should contribute to future pathological studies of SARS-CoV-2 and therapeutic development of Covid-19, particularly using recombinant S1 proteins as models. Funding Information: The authors declare that they have no conflict of interest. Declaration of Interests: The work was supported in part by the National Natural Science Foundation of China (31600650, 31671369, and 62072435), the March of Dimes Prematurity Research Center grant (22-FY18-82), and Wellcome Trust Biomedical Resource grant (218304/Z/19/Z).

Spatial variation of regional soil organic carbon (SOC) stocks and the effects of environmental factors on SOC in forest soils play an essential part in modeling the global carbon cycle and climatic change. In this study, we analyzed topsoil samples (0–30 cm) from 838 plots in a typical subtropical forest of Zhejiang Province, southeastern China, using Moran's I, geostatistics and geographical information system (GIS) techniques. The results showed that the mean SOC density in the topsoils was 71.15 Mg ha−1 and the total storage in topsoils of Zhejiang Province was 0.47 Pg (Pg = 1015 g). The spatial distribution of SOC density based on kriging interpolation and local Moran's I hotspot analysis showed a decreasing trend from the southwest to northeast area, roughly in line with Zhejiang Province's topographic feature, as well as differences in land use, economic and social development. The results herein revealed that both dominant tree species and altitude (P

Spatial pattern information of carbon (C) density and storage in forest ecosystems plays an important role in the evaluation of C sequestration potential and forest management practices. However, such information related to subtropical forests still remain poorly understood. In this study, we collected forest samples from 838 plots in a typical subtropical forest of Zhejiang Province, southeastern China. The Moran’s I, geostatistics and geographical information system (GIS) techniques were applied to reveal the spatial pattern of C stocks in forest ecosystems. The results showed that the mean C density of forest ecosystems in Zhejiang Province was 145.22 Mg ha−1, and the forest vegetation, soil, litter, and dead wood layer C densities were 27.34, 108.89, 1.79, and 1.38 Mg ha−1, respectively. The spatial distribution of forest ecosystems C density showed a decreasing trend from the southwest to northeast area, roughly in line with Zhejiang Province’s topographic feature, as well as differences in land use, forest management, economic and social development. In addition, the C storage of forest ecosystems in Zhejiang Province was approx. 877.19 Tg (1 Tg = 1012 g), with 203.88 Tg in the vegetation layer, 656.20 Tg in the soil layer, 10.84 Tg in the litter layer, and 6.27 Tg in the dead wood layer, accounting for 23.24%, 74.81%, 1.24%, and 0.71% of the total C storage, respectively. These results indicated that the soil layer is the most important contributor to C storage in subtropical forest ecosystems. The young and middle-aged forests with low C densities dominated in the study area. Therefore, in order to enhance the C sequestration potentials, forest management should focus on the selection of tree species, management of stand-age structures and implementation of sustainable afforestation and reforestation practices in the future.

Oxygen and nitrogen in the air have different magnetic properties in a magnetic field. Oxygen and nitrogen are paramagnetic gases and diamagnetic gases, and the magnetizing force received in a gradient magnetic field is in opposite directions. Based on the magnetization theory and simulation experiment analysis, this paper uses the difference in the direction and magnitude of the magnetization force of oxygen and nitrogen in a gradient magnetic field to study the force state of oxygen and nitrogen molecules in the input air in a gradient magnetic field and the oxygen mass fraction of the output gas to explore effective ways to obtain oxygen-enriched gas. The results of this study can provide oxygen-enriched gas for operations such as oxygen-enriched combustion technology and sewage treatment engineering, and improve operational efficiency.

As a main way of logistics transportation, electric logistics vehicle is beneficial to logistics industry and save energy. Parameters matching and optimization method of power transmission system for electric logistics vehicle is studied in this article. Data processing rules and principles are created for CHTC-LT working condition. Working condition and genetic algorithm are used for parameters matching and transmission ratio optimization of power transmission system. Driving range of electric logistics vehicle could be improved.

The computational fluid dynamics software fluent is used to calculate and study the hydrodynamic performance of the rotating screw in the water flow field. First, UG is used to establish different parameter calculation models, and then the slip grid method in the fluent is used to calculate the flow field state, thrust and torque of the simulated screw rod under different blade angles and number of blade turns, calculate through the above data obtain the screw rod’s pushing efficiency, and obtain the best structural parameters of the screw rod when the best pushing effect is achieved through the comparison of experimental data.

In this study, we investigated the effect of forest types changes (from coniferous and broadleaf mixed forest (CBMF) to plantation forests of bamboo (Phyllostachys pubescens forest, MBF) and hickory (Carya cathayensis forest, CHF)) combined with intensive management on soil organic carbon (SOC) and microbial community structure, using the 13C-nuclear magnetic resonance (NMR) and phospholipid fatty acid (PLFA). The results indicated that soil organic carbon significantly decreased by 30.7 and 28.5% in MBF and CHF, respectively. The aromatic C and aromaticity also significantly decreased in MBF and CHF (P 0.05). Significant changes of the soil microbial community were found after the forest type changed from CBMF to MBF and CHF. Total soil microbial PLFAs, soil bacteria PLFAs, fungus PLFAs, actinobacteria PLFAs, arbuscular mycorrhizal fungi PLFAs and protozoan PLFAs ranked as follows: CBMF > CHF > MBF (P CHF (0.66) > CBMF (0.49) (P CHF > MBF, P

The natural extracellular matrix (ECM) offers a dynamic and intricate microenvironment, which serves as a structural support and regulates cell phenotype and its function. Recently, ECM has been revealed as a favorable and biocompatible architecture for stem cell adhesion and growth in cellular therapy to treat various diseases. However, cell-derived ECM is rarely used as a culture substrate for anchorage-dependent cells, such as neural progenitor cells (NPCs), which have the potential to differentiate into basal forebrain cholinergic neurons (BFCNs) for Alzheimer's disease. Here, we report mouse embryonic fibroblast (MEF)-derived ECM, with an appropriately hydrophilic property (water contact angle of 66.8°) to mimic the neural niche for NPCs. In addition, MEF-derived ECM possesses a nanotopological surface, plentiful kinds of components and exhibits excellent adhesion properties for anchoring NPCs. Compared with a laminin-coated plate, MEF-derived ECM promotes NPC proliferation and differentiation into BFCNs by ∼1.6 fold and ∼3.1 fold, respectively, consequently enhancing the production of acetylcholine by ∼2.0 fold. This MEF-derived ECM could be a favorable cell culture carrier for NPC attachment, with great potential for applications in stem cell therapy for Alzheimer's disease.

The heavy metal pollution in soils has attracted a growing concern from environmental researchers worldwide. Heavy metals derived from E-waste dismantling process now is one of the main pollution sources. Wenling city is one of the large E-waste dismantling sites as well as a commercial grain production area in China. In this study, the Cd, Cu, Ni, Pb and Zn concentrations of paddy soils were determined to reveal the temporal trends over the past decade based on the samples collected from 2006 a (96) and 2016 a (167), respectively. From 2006 a to 2016 a, the averages values of Cd, Cu, Ni and Zn concentrations in soils increased by 0.11, 11.81, 1.01 and 6.82 mg kg−1, respectively. The average concentration of Pb in soils decreased by 14.06 mg kg−1. All of the five heavy metals shared the similar spatial distribution patterns with high-concentration values located in northwestern part of the study area. The results of principal component analysis (PCA) revealed that the E-waste was the main source of pollution for Cd, Cu, Pb and Zn in soils. The spatial patterns revealed by local Moran’ I indicated that the northwestern part were identified as priority areas for soil heavy metal remediation. During the last decade, a continuing increase trend of the concentrations of heavy metals in soils was found, however, the other parts of the study area had an obvious decrease trend of heavy metals in soils under the implementation of environmental protection policies. Therefore, sustainable remediation measures and E-waste dismantling management are necessary to reduce heavy metal concentrations in specific areas of Wenling.

The potentially toxic trace elements (PTEs) transfer characteristics in the soil-rice system plays an important role in soil quality management, and it can be used to guide the safe rice production. We collected soil and rice samples from three typical rice production areas (Nanxun, Shengzhou, Wenling in northern, central, and southern parts of Zhejiang Province, China). The controlling factors of PTEs&prime, transfer were studied for Hybrid rice and Japonica rice. The results indicated that the pH, organic matter (OM), and electrical conductivity (EC) values of Shengzhou were all lower than that of the other two production areas (Nanxun and Wenling). The concentrations of PTEs in the soils of Wenling were significantly higher than that in the other two areas, while the concentrations of PTEs in the rice of Shengzhou were significantly higher than that of Wenling and Nanxun (p &lt, 0.05). The enrichment index (EI) of PTEs were also different in the three production areas. The EIs of Cd and Zn were higher than that of Cu and Ni in the three production areas, and the EIs in Shengzhou were significantly higher than that of other two areas (p &lt, 0.05). The soil physico-chemical properties and PTEs&rsquo, fractions both played important roles in PTEs transfer in the soil-rice system. The log-linear model of EI for PTEs can predict the availability of PTEs in the soil-rice system under practical production areas. The accuracy of the model prediction of EI for Japonica rice was better than that for the Hybrid rice. The prediction model of Ni was better than that of other PTEs for both rices.

To assess the effects of long-term intensive management on soil carbon cycle and microbial functional diversity, we sampled soil in Chinese hickory (Carya cathayensis Sarg.) stands managed intensively for 5, 10, 15, and 20 years, and in reference Chinese hickory&ndash, broad-leaved mixed forest (NMF) stands. We analyzed soil total organic carbon (TOC), microbial biomass carbon (MBC), and water-soluble organic carbon (WSOC) contents, applied 13C-nuclear magnetic resonance analysis for structural analysis, and determined microbial carbon source usage. TOC, MBC, and WSOC contents and the MBC to TOC ratios were lower in the intensively managed stands than in the NMF stands. The organic carbon pool in the stands managed intensively for twenty years was more stable, indicating that the easily degraded compounds had been decomposed. Diversity and evenness in carbon source usage by the microbial communities were lower in the stands managed intensively for 15 and 20 years. Based on carbon source usage, the longer the management time, the less similar the samples from the monospecific Chinese hickory stands were with the NMF samples, indicating that the microbial community compositions became more different with increased management time. The results call for changes in the management of the hickory stands to increase the soil carbon content and restore microbial diversity.

Urban soils are strongly related to human health. In this study, Lin&rsquo, an city was chosen as a typical small-scale city with which to study the spatial variation of potentially hazardous metals (PHMs) in urban soils and their potential ecological risks using multivariate analysis, geostatistics and GIS techniques. A total of 62 soil samples were collected from the study area. The results showed that the average concentrations of total soil Mn, Cu, Zn, Pb, Cr, Cd were 439.42, 42.23, 196.80, 62.55, 63.65, 0.22 mg&middot, kg&minus, 1, respectively. Compared with the background values and the environmental quality standards, these PHMs were accumulated in urban soils to some extent. The single potential ecological risk indices of PHMs indicated that Pb and Cd had relatively high ecological risks. The pH and most of the PHMs had significant correlations (p &lt, 0.05). The principle components analysis (PCA) showed that Pb, Zn and Cu had similar pollution sources related to the vehicles&rsquo, exhaust emission, Mn and Cr were mainly from the parent materials, while Cd was from the emission of industrial manufactories. The spatial structures and distributions of PHMs and their corresponding available fractions had strong/moderate spatial autocorrelation, which were influenced by human activities.

Objectives: Elevation of anti-thyroglobulin antibodies that are primarily IgG isotype is a hallmark of Hashimoto’s thyroiditis (HT). As for IgG,it bears two conserved repertoire of N-linked glycans attached to its crystallizable fragment (Fc) at the 297 asparagine residue (Asn297). In our previous study, we found that serum TgAb IgG from HT patients exhibits higher glycosylation levels than those observed from healthy controls. Previous studies confirmed that imbalance of Th1/Th2 and Th17/Treg leading to altered immune microenvironment with elevation of certain cytokines was found in the thyroid tissue of HT, including IFN-γ, TNF-α, IL-21, IL-17A, IL-6, BAFF, APRIL. Thus, the aim of our study was to investigate the influence of the elevated cytokines on the differentiation process of B cells and the glycosylation levels of IgG. Methods: We formed a two-phase culture system in vitro to promote B cells to differentiate to antibody-secreting cells (ASCs). In the process of cell culture, B cells were co-cultured with cytokines as followed: IFN-γ, TNF-α, IL-21, IL-17A, IL-6, BAFF and APRIL. Flow cytometry was performed to identify the percentage of plasmablasts (CD38+CD27high) and plasma cells (CD20-CD138+). ELISA was used to measure the yield of IgG in culture supernatants. The glycosylation levels of secreted IgG under different stimulation conditions were detected by lectin microarray. Results: We found that IL-21, TNF-α and BAFF can significantly promote the differentiation of B cells into ASCs in vitro culture system, and augment the production of IgG to over 4-fold. In addition, cytokines affected the glycosylation modification profile of IgG diversely: 1) IL-21, IL-17A, TNF-α, BAFF significantly increased the glycosylation level of sialic acid of total IgG; 2) IFN-γ significantly increased the level of galactose; 3) IL-21, IL-17A, IFN-γ, BAFF, and APRIL significantly increased the level of mannose; 4) IL-6 significantly decreased the level of sialic acid, galactose and mannose; 5) IL-17A, IFN-γ, TNF-α, BAFF significantly increased the level of GalNAc that was a component of O-Glycan,which only exists in the hinge region of IgG3 subclass. Conclusions: The abnormally elevated cytokines in microenvironment participated in the regulation of B cell terminal differentiation process and glycosylation level of IgG, thereby involving in the pathogenesis of AITD.

Changes in immunoglobulin G (IgG) glycan structures are currently believed to closely related to the emergence of cancer. In this review, we summarize the current body of evidence suggesting that differences in serum IgG glycosylation patterns correspond to changes in multiple types of cancer. Modifications include IgG terminal N-link galactosylation, IgG core fucosylation, IgG terminal sialylation, and IgG terminal bisecting N-acetylglucosamine. IgG N-glycomic alterations represent promising novel biomarkers for non-invasive-cancer diagnosis, prognosis, and progression monitoring; they are characterized by high sensitivity and specificity, compensating for previously identified glycobiomarkers.

There is an increasing concern for potentially hazardous metals pollution, which can threaten crops production and human health. A total of 94 pairs of soil and rice samples were collected from Shengzhou City, southern China. The results showed that the mean concentrations of Cd, Cu, Ni, Pb, Zn in soils were 0.20, 28.64, 27.03, 38.51 and 98.74 mg kg − 1 , respectively. Compared with the guideline values, the potentially hazardous metals were enriched to different degrees in paddy soils of the study areas and some were contaminated with these metals. However, the potentially hazardous metals in rice remained at a safe level. The total concentrations of Cd, Cu, Ni, Pb, Zn in soils had moderate variability coefficients of 45%, 47%, 82%, 18%, 32%, respectively. Kriging interpolation procedure and the Local Moran's I index detected the locations of pollution hotspots of these five potentially hazardous metals. The concentrations of Cd, Cu, Ni, Zn in soils had a very similar spatial pattern, with contamination hotspots located together in the middle part of the study area, which is probably due to industrialization and other anthropogenic activities. The accumulation and availability of potentially hazardous metals in soil and rice is influenced by both metal concentrations in soil, and soil-plant physico-chemical properties that influence their uptake by rice. Therefore, we conclude that necessary environmental measures should be taken to control soil pollution in order to limit possible potentially hazardous metals contamination of the food chain in Shengzhou City.

Glycans are crucial to a wide range of biological processes, and their biological activities are closely related to the branching patterns of structures. Different from the simple linear chains of proteins, branching patterns of glycans are more complicated, making their identification extremely challenging. Tandem mass spectrometry (MS2) cannot provide sufficient structural information to deduce glycan branching patterns even with the assistance of various bioinformatic tools and algorithms.The promising technology to identify glycan branching patterns is multi-stage mass spectrometry (MSn). The production-relationship among MSn spectra of a glycan is essentially a tree, making deducing glycan structures from MSn spectra a great challenge. In the present study, we report an approach called glyBranch (glycan Branching pattern identification based on spectra tree) to fully exploit the information contained in the MSn spectra tree for glycan identification. Using 14 glycan standards, including 2 pairs with isomeric sequence, and 16 complex N-glycans isolated from RNase B and IgG, we demonstrated the successful application of glyBranch to branching pattern analysis. The source code of glyBranch is available at https://github.com/bigict/glyBranch/. We have also developed a web-server, which is freely accessible at http://glycan.ict.ac.cn/glyBranch/. SIGNIFICANCE: Glycans are crucial in various biological processes and their functions are closely related to the details of their structures; thus, the identification of glycan branching patterns is of great significance to biological studies. Multistage mass spectrometry (MSn) can provide detailed structural information by generating multiple-level fragments through consecutive fragmentation; however, the interpretation of numerous MSn spectra is extremely challenging. In this study, we present an approach called glyBranch (glycan Branching pattern identification based on spectra tree) to exploit the information contained in MSn spectra tree for glycan identification. This approach will greatly facilitate the automated identification of glycan structures and related biological studies.

Chinese hickory (Carya cathayensis Sarg.) is a famous woody nut in China. Currently, there is an increasing concern related to the soil heavy metal pollution in Chinese hickory plantations. In this study, a total of 188 soil samples were collected from Lin’an city, a typical Chinese hickory production area. The results showed that the average background concentrations of Cd, Cu, Zn, Pb, Ni and Cr in soils were 0.37, 40.76, 87.61, 30.10, 28.33 and 56.57 mg kg−1, respectively. The Cd and Pb concentrations in Chinese hickory plantation soils had strong variability coefficients of 186.49% and 95.42%, respectively. Compared with the background values in Zhejiang province, the heavy metals were enriched in the plantation soils. The accumulation ratio followed the order of Cu > Cd > Pb > Ni > Zn > Cr. Part of the study area was seriously contaminated by Cd and Cu, as 31.38% of soil Cd and Cu samples exceeded the second grade standardized value of Environmental Quality Standards of Soils (EQSS). The soils in our study area generally reached a moderate ecological risk, as its average RI value reached 103. Moran’s I and Kriging interpolation results revealed that all the studied heavy metals in soils had clear spatial distribution patterns. The high values of soil heavy metals (except Pb) were mainly distributed in the localities closest to the mine area. The apportionment of heavy metal pollution sources showed that the Cu, Ni and Cr in soils were mainly related to mining activities, Pb was closely related to fertilizer application, Cd and Zn were related to both. The heavy metals in soils may pose a potential threat to local ecosystem and human health. Our results implied that soil heavy metals should be tested, and risk-based management should be performed in the economic plantations in China. These maps could provide useful information for forestry and environmental management.

Outliers are classified as global outliers and spatial outliers. Up to now, there is little information about the outliers especially the spatial outliers and their influence on the spatial structure modeling of soil properties. A total of 537 soil samples were collected based on a 30 × 30 m grid in a permanent dairy farm in southeast Ireland. Graphic methods of histogram and box plot combined with Moran’s I were applied to detect the outliers of soil phosphorus (P). Sixteen outliers (5 global outliers and 11 spatial outliers) of soil P were found in the study area. Compared to the raw data, the data with global outliers excluded always had the larger global Moran’s I value indicating a stronger spatial autocorrelation. Clear spatial clusters (High–High and Low–Low clusters) were observed based on local Moran’s I. The High–High spatial clusters were located around the main farm yard and near the traffic route due to more intensive management by farmers. The Low–Low spatial clusters were mainly close to the river. For these areas, P fertilizer or slurry should be applied for healthy grass growth. The dataset with outliers excluded had a reliable semi-variogram model with a low nugget/sill ratio (32.4 %), which was closed to its corresponding transformed data (30.5 %). The cross-validation results revealed that the dataset without outliers had the strongest linear regression model (r = 0.768), indicating that the outliers played an important role in the spatial structure modeling.

A total of 16 novel polymorphic microsatellite marker loci were isolated from a genomic library of Euchiloglanis kishinouyei and further characterized using a sample from a wild population consisting of 40 individuals. The number of alleles among loci ranged from three to 17 and the observed and expected heterozygosities ranged from 0·030 to 0·950 and 0·163 to 0·980, respectively. The average polymorphic information content (PIC) of all loci was 0·450.

Field-scale variation of soil phosphorus (P) information is very important for P fertilizer application and its soil sampling design in grassland. A total of 108 soil samples were collected from a long-term (41 years) grazed grassland P experiment field at Teagasc, Johnstown Castle, Wexford, Ireland, in March 2009. There were six P treatments (P0-0, P0-30, P15-15, P15-5, P30-30, and P30-0) since 1968, with changes since 1999. Each treatment had 6 replicate plots (a total of 36 plots, 3 soil samples per plot). The samples were analyzed for available (Morgan’s) P, potassium (K), magnesium (Mg), lime requirement (LR), and pH. The highest mean available P concentration was found in the P30-30 (30 kg P ha−1 pre- and post-1998) plots, and the lowest mean available P concentration was found in the P0-0 (no P fertilizer since 1968) plots. Significant differences of mean P, Mg, LR, and pH values in different treatments were observed. There was a positive proportional effect for both the 36 plots and the 6 treatmen...