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8 results on '"Kellnerová, Sára"'

1. Detection of Cleaved Stx2a in the Blood of STEC-Infected Patients

Author

Varrone, Elisa, primary, Carnicelli, Domenica, additional, He, Xiaohua, additional, Grasse, Marco, additional, Stampfer, Karin, additional, Huber, Silke, additional, Kellnerová, Sára, additional, Tazzari, Pier Luigi, additional, Ricci, Francesca, additional, Paterini, Paola, additional, Ardissino, Gianluigi, additional, Morabito, Stefano, additional, Orth-Höller, Dorothea, additional, Würzner, Reinhard, additional, and Brigotti, Maurizio, additional

Published
2023
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2. Enzymatic Cleavage of Stx2a in the Gut and Identification of Pancreatic Elastase and Trypsin as Possible Main Cleavers

Author

Kellnerová, Sára, primary, Huber, Silke, additional, Massri, Mariam, additional, Fleischer, Verena, additional, Losso, Klemens, additional, Sarg, Bettina, additional, Kremser, Leopold, additional, Talasz, Heribert, additional, He, Xiaohua, additional, Varrone, Elisa, additional, Brigotti, Maurizio, additional, Ardissino, Gianluigi, additional, Orth-Höller, Dorothea, additional, and Würzner, Reinhard, additional

Published
2023
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4. Systemic Inflammation and Complement Activation Parameters Predict Clinical Outcome of Severe SARS-CoV-2 Infections

Author

Huber, Silke, primary, Massri, Mariam, additional, Grasse, Marco, additional, Fleischer, Verena, additional, Kellnerová, Sára, additional, Harpf, Verena, additional, Knabl, Ludwig, additional, Heiner, Tatjana, additional, Kummann, Moritz, additional, Neurauter, Magdalena, additional, Rambach, Günter, additional, Speth, Cornelia, additional, and Würzner, Reinhard, additional

Published
2021
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5. Shiga Toxin 2a Binds to Complement Components C3b and C5 and Upregulates Their Gene Expression in Human Cell Lines

Author

Kellnerová, Sára, Chatterjee, Sneha, Bayarri-Olmos, Rafael, Justesen, Louise, Talasz, Heribert, Posch, Wilfried, Kenno, Samyr, Garred, Peter, Orth-Höller, Dorothea, Grasse, Marco, Würzner, Reinhard, Kellnerová, Sára, Chatterjee, Sneha, Bayarri-Olmos, Rafael, Justesen, Louise, Talasz, Heribert, Posch, Wilfried, Kenno, Samyr, Garred, Peter, Orth-Höller, Dorothea, Grasse, Marco, and Würzner, Reinhard

Abstract

Enterohemorrhagic Escherichia coli (EHEC) infections can cause EHEC-associated hemolytic uremic syndrome (eHUS) via its main virulent factor, Shiga toxins (Stxs). Complement has been reported to be involved in the progression of eHUS. The aim of this study was to investigate the interactions of the most effective subtype of the toxin, Stx2a, with pivotal complement proteins C3b and C5. The study further examined the effect of Stx2a stimulation on the transcription and synthesis of these complement proteins in human target cell lines. Binding of Stx2a to C3b and C5 was evaluated by ELISA. Kidney and gut cell lines (HK-2 and HCT-8) were stimulated with varied concentrations of Stx2a. Subsequent evaluation of complement gene transcription was studied by real-time PCR (qPCR), and ELISAs and Western blots were performed to examine protein synthesis of C3 and C5 in supernatants and lysates of stimulated HK-2 cells. Stx2a showed a specific binding to C3b and C5. Gene transcription of C3 and C5 was upregulated with increasing concentrations of Stx2a in both cell lines, but protein synthesis was not. This study demonstrates the binding of Stx2a to complement proteins C3b and C5, which could potentially be involved in regulating complement during eHUS infection, supporting further investigations into elucidating the role of complement in eHUS pathogenesis.

Published
2021

6. Shiga Toxin 2a Binds to Complement Components C3b and C5 and Upregulates Their Gene Expression in Human Cell Lines

Author

Kellnerová, Sára, primary, Chatterjee, Sneha, additional, Bayarri-Olmos, Rafael, additional, Justesen, Louise, additional, Talasz, Heribert, additional, Posch, Wilfried, additional, Kenno, Samyr, additional, Garred, Peter, additional, Orth-Höller, Dorothea, additional, Grasse, Marco, additional, and Würzner, Reinhard, additional

Published
2020
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7. Apyrase decreases phage induction and Shiga toxin release from E. coliO157:H7 and has a protective effect during infection

Author

Arvidsson, Ida, Tontanahal, Ashmita, Johansson, Karl, Kristoffersson, Ann-Charlotte, Kellnerová, Sára, Berger, Michael, Dobrindt, Ulrich, and Karpman, Diana

Abstract

ABSTRACTShiga toxin (Stx)-producing enterohemorrhagic Escherichia coli(EHEC) cause gastrointestinal infection and, in severe cases, hemolytic uremic syndrome which may lead to death. There is, to-date, no therapy for this infection. Stx induces ATP release from host cells and ATP signaling mediates its cytotoxic effects. Apyrase cleaves and neutralizes ATP and its effect on Stx and EHEC infection was therefore investigated. Apyrase decreased bacterial RecA and dose-dependently decreased toxin release from E. coliO157:H7 in vitro, demonstrated by reduced phage DNA and protein levels. The effect was investigated in a mouse model of E. coliO157:H7 infection. BALB/c mice infected with Stx2-producing E. coliO157:H7 were treated with apyrase intraperitoneally, on days 0 and 2 post-infection, and monitored for 11 days. Apyrase-treated mice developed disease two days later than untreated mice. Untreated infected mice lost significantly more weight than those treated with apyrase. Apyrase-treated mice exhibited less colonic goblet cell depletion and apoptotic cells, as well as lower fecal ATP and Stx2, compared to untreated mice. Apyrase also decreased platelet aggregation induced by co-incubation of human platelet-rich-plasma with Stx2 and E. coliO157 lipopolysaccharide in the presence of collagen. Thus, apyrase had multiple protective effects, reducing RecA levels, stx2and toxin release from EHEC, reducing fecal Stx2 and protecting mouse intestinal cells, as well as decreasing platelet activation, and could thereby delay the development of disease.

Published
2022
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8. Funcionalización de nanopartículas virales (VNPs) derivadas de un virus de plantas con fines de protección vegetal.

Author

Kellnerová, Sára and Ponz Ascaso, Fernando

Abstract

Copyright of Revista Complutense de Ciencias Veterinarias is the property of Universidad Complutense de Madrid and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2018
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