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The mechanisms of postacute medical conditions and unexplained symptoms after SARS-CoV-2 infection [Long Covid (LC)] are incompletely understood. There is growing evidence that viral persistence, immune dysregulation, and T cell dysfunction may play major roles. We performed whole-body positron emission tomography imaging in a well-characterized cohort of 24 participants at time points ranging from 27 to 910 days after acute SARS-CoV-2 infection using the radiopharmaceutical agent [18F]F-AraG, a selective tracer that allows for anatomical quantitation of activated T lymphocytes. Tracer uptake in the postacute COVID-19 group, which included those with and without continuing symptoms, was higher compared with prepandemic controls in many regions, including the brain stem, spinal cord, bone marrow, nasopharyngeal and hilar lymphoid tissue, cardiopulmonary tissues, and gut wall. T cell activation in the spinal cord and gut wall was associated with the presence of LC symptoms. In addition, tracer uptake in lung tissue was higher in those with persistent pulmonary symptoms specifically. Increased T cell activation in these tissues was also observed in many individuals without LC. Given the high [18F]F-AraG uptake detected in the gut, we obtained colorectal tissue for in situ hybridization of SARS-CoV-2 RNA and immunohistochemical studies in a subset of five participants with LC symptoms. We identified intracellular SARS-CoV-2 single-stranded spike protein-encoding RNA in rectosigmoid lamina propria tissue in all five participants and double-stranded spike protein-encoding RNA in three participants up to 676 days after initial COVID-19, suggesting that tissue viral persistence could be associated with long-term immunologic perturbations.

Electrification to reduce or eliminate greenhouse gas emissions is essential to mitigate climate change. However, a substantial portion of our manufacturing and transportation infrastructure will be difficult to electrify and/or will continue to use carbon as a key component, including areas in aviation, heavy-duty and marine transportation, and the chemical industry. In this Roadmap, we explore how multidisciplinary approaches will enable us to close the carbon cycle and create a circular economy by defossilizing these difficult-to-electrify areas and those that will continue to need carbon. We discuss two approaches for this: developing carbon alternatives and improving our ability to reuse carbon, enabled by separations. Furthermore, we posit that co-design and use-driven fundamental science are essential to reach aggressive greenhouse gas reduction targets.

Abstract Objective Young adults experiencing homelessness often suffer from adverse mental health outcomes and suicide is a leading cause of death. The objective of this study is to examine service use and psychosocial risk factors for suicide, in relation to suicide risk assessment, to inform strategies for engaging youth in suicide prevention services. Methods A cross-sectional analysis of youth enrolled in a supportive housing randomized clinical trial. We categorized suicide risk into three groups (low-, moderate-, and high-risk) based on suicidal ideation and past-year suicide attempt. The service use patterns across these groups are described, as well as other psychosocial risk factors (psychiatric comorbidity, depressive symptoms, substance use, and sleep impairment). Results Among 193 enrolled youth, 126 (65.3%), 32 (16.6%), and 35 (18.1%) were categorized as low-risk, moderate-risk, and high-risk for suicide, respectively. A high proportion of youth reported ever having been diagnosed with a psychiatric disorder (57.5%) and cannabis was the most heavily used substance. However, only a minority of youth received medical care or mental health services in the past 3 months, 40% and 34%, respectively. Even fewer youth received mental/emotional health services in the past 3 months (15%), though use was highest among the high-risk group (34%). Conclusion Understanding the needs of youth experiencing homelessness who are at risk for suicide is critical to developing interventions to alleviate their risk for suicide. Given that the use of medical and mental health care is low among this population, more active outreach strategies may be warranted to deliver prevention interventions.

Pancreatic cystic lesions (PCLs) are frequent incidental findings with an increasing prevalence with age. For these significant lesions, accurate characterization of cyst type and prediction of the risk of malignant progression are crucial for specific management, such as deciding to monitor a lesion or pursue surgical intervention. Fortunately, endoscopy-based diagnostic and therapeutic techniques like endoscopic ultrasound with fine needle aspiration, confocal endomicroscopy, through-the-needle biopsy, contrast-enhanced endoscopic ultrasound, ablation, and pancreatoscopy have enabled increasingly accurate diagnoses of PCLs. Surgical management should be considered in certain cases. This narrative review’s objective is to appraise and synthesize the salient literature on the endoscopic and surgical management of PCLs to aid clinician decision-making. We analyze the current data and explore the benefits, challenges, and future prospects of endoscopy and surgery for pancreatic cysts.

Hands-on learning activities enhance course outcomes in college courses based on increased exam scores and student satisfaction; however, online courses pose challenges for creating hands-on learning opportunities. The objective of this study was to compare online and on-campus students' perception and performance on a soybean processing and products module with a laboratory component. The modules included identical lectures and discussion board questions. Students then participated in a hands-on laboratory exercise at home or on-campus investigating the effect of added soy protein at various levels on muffins physical and sensory properties. Baking kits were mailed to online students. All students were given detailed written instructions on how to prepare muffins for the exercise. Upon completion of the laboratory exercise, students wrote a scientific abstract on their findings. At the end of the module, all students (n=194) completed an assessment survey about their perception of the module. Seventy-two percent of online students met or exceeded expectations on the abstract assignment while 92% of on-campus students met or exceeded expectations. In both courses, ninety percent of students agreed or strongly agreed the laboratory improved their ability to apply knowledge to practical issues. Regarding knowledge gained, students reported an increased awareness of products that contain soy and methods for processing soybeans into ingredients. A common response was that students would be more likely to try soy products in the future because they were less concerned with off flavors and interested in the nutritional benefits of soy products.

Our initial impetus for this project stems from the development of UNCP's Indigenous Cultures & Communities (ICC) graduation requirement. We recognize the efforts of individual faculty representing disciplines in the arts, education, humanities, library, and nursing to redesign class activities and courses. Our purpose is to share models and templates for readers' use and adaptation in incorporating Indigenous-centered pedagogies in their own courses. We have curated practical and philosophical methods for engaging Indigenous knowledge and using Indigenous centered pedagogies in college courses and curricula. We believe that faculty efforts to redesign their courses in support of a newly instituted graduation requirement focused on Indigenous cultures and communities will benefit all students participating in these courses regardless of their background.

PURPOSE: Herpes zoster ophthalmicus (HZO) after COVID-19 vaccination has been reported in numerous case studies. However, no large-scale epidemiologic studies have been conducted to date. The purpose of this study was to determine whether COVID-19 vaccination is associated with an increased risk of HZO. DESIGN: Retrospective before-and-after risk interval analysis. METHODS: RESULTS: In total, 1,959,157 patients received a dose of a COVID-19 vaccine during the study period and met eligibility criteria. A total of 80 individuals without a prior history of HZO were included in the analysis because they developed HZO in the risk or control period. Patients had a mean age of 54.0 years (SD = 12.3 years). There were 45 cases of HZO in the risk interval after COVID-19 vaccination. There was not an increased risk of HZO after vaccination with BNT162b2 (IRR = 0.90, 95% CI: 0.49-1.69, P = .74), mRNA-1273 (IRR = 0.74, 95% CI: 0.36-1.54, P = .42), or Ad26.COV2.S (IRR = 0.50, 95% CI: 0.07-2.56, P = .42). CONCLUSIONS: This study found no evidence of increased risk of HZO after COVID-19 vaccination, providing reassurance for patients and providers who may be concerned about the safety profile of the COVID-19 vaccines.

Long COVID (LC) occurs after at least 10% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, yet its etiology remains poorly understood. We used omic assays and serology to deeply characterize the global and SARS-CoV-2-specific immunity in the blood of individuals with clear LC and non-LC clinical trajectories, 8 months postinfection. We found that LC individuals exhibited systemic inflammation and immune dysregulation. This was evidenced by global differences in T cell subset distribution implying ongoing immune responses, as well as by sex-specific perturbations in cytolytic subsets. LC individuals displayed increased frequencies of CD4+ T cells poised to migrate to inflamed tissues and exhausted SARS-CoV-2-specific CD8+ T cells, higher levels of SARS-CoV-2 antibodies and a mis-coordination between their SARS-CoV-2-specific T and B cell responses. Our analysis suggested an improper crosstalk between the cellular and humoral adaptive immunity in LC, which can lead to immune dysregulation, inflammation and clinical symptoms associated with this debilitating condition.

Interspecific interactions are crucial in shaping ecosystem dynamics (Connell 1983, Tilman 1987, Barbosa and Castellanos 2005). Non-native ungulates have been introduced across the globe (Ferretti and Lovari 2014, Volery et al. 2021) and in environments where multiple invasives thrive, these co-occurring sympatric species may shift spatiotemporal patterns to minimize interspecific competition (Schoener 1974, Chesson 2000). Spatiotemporal shifts can lead to cascading effects to the native flora and fauna (Simberloff and Von Holle 1999). Given the potential negative impacts, understanding these interactions is vital for effective management of these ecosystems. Two invasive ungulates, the wild pig (Sus scrofa) and the Philippine deer (Rusa marianna), have inhabited much of Guam since their introduction in the 1600s and 1700s (Intoh 1986, Wiles et al. 1999) and both have been associated with significant ecological damage throughout the island (Conroy 1989). Despite sharing similar invasive roles, they seemingly coexist throughout areas of Guam. Subsequently, efforts are increasingly put forth to remove both species from partitioned areas at one time. To aid these efforts and understand invasive species interactions, we studied the seasonal spatiotemporal patterns of wild pigs and Philippine deer during February 2021-March 2022. Specifically, we used GPS collars from 39 wild pigs and 22 deer to examine spatial and temporal partitioning and assess dynamic spatiotemporal interactions between interspecific dyads at seasonal scales. We investigated spatial partitioning of wild pigs and deer by estimating the spatial overlap between home ranges and core areas of interspecific neighboring dyads. We estimated temporal overlap in diel activity to evaluate temporal partitioning between the species. Lastly, we examined dynamic spatiotemporal interactions, those that occur simultaneously in space and time, by estimating movement interactions between neighboring interspecific dyads to understand attraction and avoidance. We found spatial overlap between the species decreased significantly in core areas compared to home ranges in both seasons. Within home ranges, deer were approximately 3 times more likely to be within pig ranges than vice versa. This effect diminished at core areas such that deer were only 1.3× more likely to be within pig core ranges than vice versa. Temporal overlap of activity between wild pigs and deer was very high during dry and wet seasons, with overlapping activity peaks during crepuscular hours. At a critical distance threshold of 50m, we estimated 77 movement interactions from 58 dyad pairs over 3 seasons and found that only 2 (2.6%) and 4 (5.2%) movement interactions were considered avoidant and attractive, respectively, and the remaining 71 interactions (92.2%) were neutral.

The associations between longitudinal dynamics and the breadth of SARS-CoV-2 neutralizing antibody (nAb) response with various Long COVID phenotypes before vaccination are not known. The capacity of antibodies to cross-neutralize a variety of viral variants may be associated with ongoing pathology and persistent symptoms. We measured longitudinal neutralizing and cross-neutralizing antibody responses to pre- and post-SARS-CoV-2 Omicron variants in participants infected early in the COVID-19 pandemic, before widespread rollout of SARS-CoV-2 vaccines. Cross-sectional regression models adjusted for clinical covariates and longitudinal mixed-effects models were used to determine the impact of the breadth and rate of decay of neutralizing responses on the development of Long COVID symptoms, as well as Long COVID phenotypes. We identified several novel relationships between SARS-CoV-2 antibody neutralization and the presence of Long COVID symptoms. Specifically, we show that, although nAb responses to the original, infecting strain of SARS-CoV-2 were not associated with Long COVID in cross-sectional analyses, cross-neutralization ID50 levels to the Omicron BA.5 variant approximately 4 months following acute infection was independently and significantly associated with greater odds of Long COVID and with persistent gastrointestinal and neurological symptoms. Longitudinal modeling demonstrated significant associations in the overall levels and rates of decay of neutralization capacity with Long COVID phenotypes. A higher proportion of participants had antibodies capable of neutralizing Omicron BA.5 compared with BA.1 or XBB.1.5 variants. Our findings suggest that relationships between various immune responses and Long COVID are likely complex but may involve the breadth of antibody neutralization responses.

IntroductionCardiovascular fat is a novel risk factor that may link to dementia. Fat volume and radiodensity are measurements of fat quantity and quality, respectively. Importantly, high fat radiodensity could indicate healthy or adverse metabolic processes.MethodsThe associations of cardiovascular fat (including epicardial, paracardial, and thoracic perivascular adipose tissue [PVAT]) quantity and quality assessed at mean age of 51 with subsequent cognitive performance measured repeatedly over 16 years of follow-up were examined using mixed models among 531 women.ResultsHigher thoracic PVAT volume was associated with a higher future episodic memory (β[standard error (SE)] = 0.08 [0.04], P = 0.033), while higher thoracic PVAT radiodensity with lower future episodic (β[SE] = -0.06 [0.03], P = 0.045) and working (β[SE] = -0.24 [0.08], P = 0.003) memories. The latter association is prominent at higher volume of thoracic PVAT.DiscussionMid-life thoracic PVAT may have a distinct contribution to future cognition possibly due to its distinct adipose tissue type (brown fat) and anatomical proximity to the brain circulation.HighlightsHigher mid-life thoracic perivascular adipose tissue (thoracic PVAT) volume is related to a better future episodic memory in women. Higher mid-life thoracic PVAT radiodensity is related to worse future working and episodic memories. Negative association of high thoracic PVAT radiodensity with working memory is prominent at higher thoracic PVAT volume. Mid-life thoracic PVAT is linked to future memory loss, an early sign of Alzheimer's disease. Mid-life women's epicardial and paracardial fat are not related to future cognition.

Serosurveys are a key resource for measuring severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) population exposure. A growing body of evidence suggests that asymptomatic and mild infections (together making up over 95% of all infections) are associated with lower antibody titers than severe infections. Antibody levels also peak a few weeks after infection and decay gradually. We developed a statistical approach to produce estimates of cumulative incidence from raw seroprevalence survey results that account for these sources of spectrum bias. We incorporate data on antibody responses on multiple assays from a postinfection longitudinal cohort, along with epidemic time series to account for the timing of a serosurvey relative to how recently individuals may have been infected. We applied this method to produce estimates of cumulative incidence from 5 large-scale SARS-CoV-2 serosurveys across different settings and study designs. We identified substantial differences between raw seroprevalence and cumulative incidence of over 2-fold in the results of some surveys, and we provide a tool for practitioners to generate cumulative incidence estimates with preset or custom parameter values. While unprecedented efforts have been launched to generate SARS-CoV-2 seroprevalence estimates over this past year, interpretation of results from these studies requires properly accounting for both population-level epidemiologic context and individual-level immune dynamics.

Study region: A 706 km2 watershed located in a heavily industrialized region near Fox Creek, Alberta, Canada. Study focus: Petroleum exploration has increasingly disturbed boreal ecosystems in North America. Seismic lines are one of the major footprints of the petroleum industry: they form cleared linear corridors in forests and their regeneration is generally poor. For this study, field measurements of soil, vegetation, water and snow were conducted on 5 paired (seismic line and adjacent undisturbed area) sites located in lowland and upland ecosites, and a 1-D physically based hydrologic model, the Simultaneous Heat and Water Model (SHAW), was used to investigate the impacts of seismic lines on the water budget over a year. New hydrological insights for the region: Field measurements indicate that parameters, including soil water content and temperature, vary according to a complex combination of factors and are highly site-specific between seismic lines and undisturbed areas. Hydrological modeling of the two ecosites (lowland and upland) showed up to a 33 % reduction in evapotranspiration and a decrease in percolation (leading to none) below the root zone on seismic lines. Considering that there are 2300 km of seismic lines in the study area and that 36 % of the forest has been cleared for industrial activities, these results suggest that the water budget is impacted by anthropogenic lineaments. Climate change will likely intensify these impacts.