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Purpose: This investigation sought to determine industry perceptions of postgraduate strength and conditioning (S&C) degrees to understand whether graduates are equipped for the demands of sport performance service roles. Methods: Survey data were collected from 111 participants employed as performance staff or in a role that recruits and supervises performance staff. The survey consisted of 3 main sections: (1) perceptions of career-development opportunities in S&C, (2) perception of S&C postgraduate programs, and (3) perceptions of employability in S&C. Exploratory factor analysis was conducted to identify the key factors considered to be of greatest relevance to career progression in S&C. Results: A 2-factor solution was achieved for each of the 3 sections, resulting in 6 total factors. These factors are Academic and Professional Development, Mentorship and Sport Diversity, Student Preparation, Require Greater Emphasis, Testing and Training, and Personal and Professional Growth. Conclusions: Postgraduate S&C programs require a broad range of placement/internship opportunities to (1) provide diverse experiences, (2) allow students to build contacts and develop professional networks, (3) gain exposure to working in high-performance environments and multidisciplinary teams, and (4) access high-quality mentors. Alongside the ability to deliver training and testing, graduates should be equipped with strong organizational and relationship-building skills. Improved graduate capabilities can raise the standards of the profession and result in enhanced service provision to athletes. [ABSTRACT FROM AUTHOR]

The recent rise in professionalisation and institutionalisation of competitive surfing has resulted in a dramatic increase in the use of alternative training modalities. These are often employed in an attempt to increase exposure to surf-like activities when appropriate ocean conditions are not available. It is commonly accepted that practice sessions should be grounded in theory, with training content informed by a clear scientific rationale. Despite this, research is yet to offer surfing coaches and surfers effective strategies to assist in implementing appropriate 'off water' training modalities. It is widely accepted that integrating a representative learning design is crucial towards the transfer of performance to competition environments. Therefore, the aim of this paper is to promote representative learning design (RLD) as a contemporary, principled framework that can underpin the creation of surfing training design and performance. Examples of constraints relevant to surfing are considered, and the efficacy and rationale of popular training methods are challenged. Finally, practical implications and coaching tools to underpin the implementation of representative learning design in surf training are provided. [ABSTRACT FROM AUTHOR]

Macrophages undergo metabolic changes during activation that are coupled to functional responses. The gram negative bacterial product lipopolysaccharide (LPS) is especially potent at driving metabolic reprogramming, enhancing glycolysis and altering the Krebs cycle. Here we describe a role for the citrate-derived metabolite malonyl-CoA in the effect of LPS in macrophages. Malonylation of a wide variety of proteins occurs in response to LPS. We focused on one of these, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). In resting macrophages, GAPDH binds to and suppresses translation of several inflammatory mRNAs, including that encoding TNFα. Upon LPS stimulation, GAPDH undergoes malonylation on lysine 213, leading to its dissociation from TNFα mRNA, promoting translation. We therefore identify for the first time malonylation as a signal, regulating GAPDH mRNA binding to promote inflammation.

Purpose: Mental fatigue causes decreases in aspects of athletes' performance. Elite coaches commonly undertake cognitively demanding tasks and are seemingly at similar risk of subsequent performance impairment. However, elite sport coaches' experiences of mental fatigue, alongside other markers of psychobiological stress, have yet to be quantified. Methods: Three elite coaching and performance staff (2 women and 1 man) provided 100-mm visual analog scale ratings of mental fatigue, physical fatigue, readiness to perform, and salivary samples for later cortisol (sCort) and alpha-amylase (sAA) analysis. Data were obtained on the same morning each week across a 16-week preseason. Data were subset by individual coach for descriptive and repeated-measures correlational analyses. Results: Fluctuating mental fatigue was observed over the 16 weeks (min–max; coach 1 = 25–86 AU; coach 2 = 0–51 AU; and coach 3 = 15 − 76 AU). Elevated levels of mental fatigue were reported at multiple time points, with individual variability observed. sCort (in nanomoles per liter), sAA (in micromoles per liter), and sAA:sCort indicated that coaches experienced psychophysiological stress (min–max; coach 1 sCort = 8.42–17.31, sAA = 52.40–113.06, sAA:sCort = 3.20–12.80; coach 2 sCort = 4.20–9.70, sAA = 158.80–307.20, sAA:sCort = 21.10–61.70; and coach 3 sCort = 6.81−19.66, sAA = 86.55–495.85, sAA:sCort = 4.90–35.50). A significant inverse relationship between mental fatigue and readiness to perform (r = −.44 [−.64 to −.17], P =.002) was identified. Conclusions: Elite sport coaches report elevated instances of mental fatigue during a preseason training period. Those involved in elite sports should act to understand the presence and potential subsequent impacts of staff mental fatigue and consider management or mitigation strategies. Optimization of the cognitive performance of coaches and performance staff presents as a potential source of competitive advantage. [ABSTRACT FROM AUTHOR]

Montmorency cherry (MC) can improve endurance performance, but optimal pre‐exercise timing of supplementation and influence of training status on efficacy are unknown. We investigated the effect of MC concentrate ingestion between 30‐ and 150‐min pre‐exercise in trained and recreational cyclists on 15‐km time trial (TT) performance and exercise economy. Twenty participants (10 recreationally active, RA; 10 trained, T) completed 10 min of steady‐state exercise (SSE) at 40%Δ (SSE) and a TT on four separate occasions following an unsupplemented (US), 30‐, 90‐ or 150‐min pre‐exercise Montmorency cherry concentrate (MCC) supplementation conditions (MCC30/90/150min). Venous and capillary blood samples were taken at regular intervals pre‐ and post‐SSE and TT. MCC significantly improved TT performance, but not exercise economy. The greatest improvement in performance occurred following MCC90min compared to US (US 1603.1 ± 248 s vs. MCC90min 1554.8 ± 226.7 s, 2.83% performance improvement). Performance was significantly enhanced for trained (US 1496.6 ± 173.1 s vs. MCC90min 1466.8 ± 157.6 s) but not recreationally active participants. Capillary [lactate] and heart rate were significantly greater during the TT for the 90‐min dose timing (p < 0.05). In the MCC30min and MCC90min conditions, plasma ferulic (US 8.71 ± 3.22 nmol. L−1 vs. MCC30min 15.80 ± 8.69 nmol. L−1, MCC90min 12.65 ± 4.84 nmol. L−1) and vanillic acid (US 25.14 ± 10.91 nmol.L−1 vs. MCC30min 153.07 ± 85.91 nmol. L−1, MCC90min 164.58 ± 59.06 nmol. L−1) were significantly higher pre‐exercise than in US and MCC150min conditions (p < 0.05). There was no significant change in muscle oxygenation status or plasma nitrite/nitrate concentration. MCC supplementation enhanced endurance exercise performance optimally when consumed ∼90 min pre‐exercise producing maximal plasma phenolic metabolites during exercise. The ergogenic effect was greater for trained participants. Highlights: Acute single‐dose supplementation of Montmorency cherry concentrate (MCC) (>800 mg polyphenols) improved endurance cycling time trial (TT) performance by 2.83% or 48.3 s.Supplementation 90 min prior to exercise produced the greatest increases in plasma phenolic metabolites and improvement in performance, suggesting the ergogenic effects are mediated by these metabolites.Trained but not recreationally active (RA) participants experienced ergogenic effects from cherry supplementation throughout the TT. [ABSTRACT FROM AUTHOR]

STUDY QUESTION Does receptor for advanced glycation end products (RAGE) on the surface membrane of the sperm cell function as a biomarker of low-quality sperm? SUMMARY ANSWER Membrane-bound RAGE at a cellular level directly correlates with low sperm motility, high cell permeability, decreased mitochondrial function, DNA fragmentation, and higher levels of apoptosis. WHAT IS KNOWN ALREADY RAGE has previously been measured by ELISA in low-quality sperm in diabetic men and has been shown to correlate with DNA fragmentation (terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay). STUDY DESIGN, SIZE, DURATION Semen samples were recovered from 60 non-obese, non-diabetic and non-smoking subjects, washed with fresh media, and analysed directly or purified further by differential gradient centrifugation (DGC) or fractionated by direct swim-up before being analysed for sperm motility and molecular health parameters, including cell membrane permeability, cell death, mitochondrial membrane potential, DNA fragmentation, and RAGE protein expression. PARTICIPANTS/MATERIALS, SETTING, METHODS Sperm motility assessments were carried out by computer-assisted sperm analysis (CASA) on 1000 spermatozoa for washed samples and 300 spermatozoa for purified samples. Molecular sperm health parameters were evaluated using flow cytometry with the use of the following markers: DAPI for cell membrane permeability, Annexin V/DAPI for cell death (apoptosis and necrosis), MitoTracker® Red CMXRos for mitochondrial membrane potential, TUNEL assay for DNA fragmentation and 8-hydroxy-2-deoxyguanosine for identification of oxidative damage to sperm DNA, and contrasted to membrane-bound RAGE expression levels, which were evaluated using an anti-RAGE monoclonal mouse antibody. MAIN RESULTS AND THE ROLE OF CHANCE RAGE protein was shown to be present on the acrosomal and equatorial regions of sperm, with the levels of membrane bound receptor strongly correlating with poor sperm health across all parameters tested; motility (R 2 = 0.5441, P  < 0.0001) and mitochondrial membrane potential (R 2 = 0.6181, P  < 0.0001) being of particular note. The analysis was performed at a single cell level thereby removing confounding complications from soluble forms of the RAGE protein that can be found in seminal plasma. The expression of the RAGE protein was shown to be stable over time and its levels are therefore not subject to variation in sample handling or preparation time. LARGE SCALE DATA N/A. LIMITATIONS, REASONS FOR CAUTION Inclusion criteria for this study were non-diabetic, non-obese and non-smoking participants to assess the distribution of RAGE expression in the general population, thereby excluding disease conditions that may increase RAGE expression in sperm or contribute to low sperm quality. The study does not address how RAGE expression may be affected in other patient subpopulations or disease states associated with male infertility. Sperm analysis by flow cytometry is not amenable to the study of males with a low sperm count. WIDER IMPLICATIONS OF THE FINDINGS Results of this study suggest that RAGE expression is a molecular maker of sperm cell health, which may be used for improvements in assisted reproduction through the removal of RAGE expressing sperm and facilitate in the diagnoses of unexplained infertility through its use as a biomarker of male infertility. STUDY FUNDING/COMPETING INTEREST(S) The study was funded by the Irish Research Council under the Government of Ireland Programme (GOIPG/2015/3729) and the Enterprise Ireland Innovation Partnership Programme (IP-2020-0952). All authors declare no competing interests. [ABSTRACT FROM AUTHOR]

A rugby league player's ability to develop or maintain lower-body power will be significantly influenced by the resistance and field-based training loads completed. This study aimed to examine the power changes across a rugby league season and to investigate the relationship of concurrent training and training experience on power changes. Eighteen rugby league players participated in this study. Lower-body power was evaluated using a countermovement jump throughout the season. Four measures of external training loads were collected. A Friedman's test was used to assess differences in power throughout different phases of the season. A series of Spearman's rank-order correlations were conducted to assess the relationship between percentage changes in power during the training block, external training loads, and training experience. Countermovement jump peak velocity significantly decreased from the start (p = 0.006) and end of pre-season (p = 0.022) to the late competition. Correlation analysis revealed a large significant association between early competition field-based external loads (relative (rs = -0.571) and total distance (rs = -0.628)) and change in peak power. Very large significant negative relationships were also observed between mid-competition volume load with change in peak force (rs = -0.825) and peak power (rs = -0.736). Training experience was not significantly associated with changes in muscular power variables throughout this study. Coaches should seek to monitor changes in peak power when competition begins and to assist with decision-making for potential adjustments to running volume and intensity during field-based sessions. [ABSTRACT FROM AUTHOR]

The term 'priming exercise' has recently been introduced to describe exercise stimuli prescribed to enhance performance within 1–48 hours of that stimulus. Despite evidence of these activities being utilised in competitive settings, the reasoning underpinning the application of priming exercise is not yet clear. Therefore, this study aimed to explore practitioners' perceptions about using priming exercise as a pre-competition strategy to improve performance. Semi-structured interviews were conducted with 28 practitioners involved in prescribing training programs to elite Australian athletes. Reflexive thematic analysis revealed six organising themes (i.e. 'limited and inapplicable research findings', 'consideration of athletes' training age', risk-return of priming session outcomes', 'logistical concerns', 'buy-in' and 'priming exercise prescription') later grouped into a global theme, representing the 'challenges of applying priming exercise'. The findings highlight contextual factors influencing practitioners' use of priming exercise and, based on these considerations, draw attention to the apparent uncertainty about whether these stimuli can be prescribed effectively to improve competition performance in sports. In addition to gaining insight into applied priming strategies, the present findings may be used to inform externally valid and relevant study designs that subsequently guide practice. [ABSTRACT FROM AUTHOR]

Purpose: This study examined the relationships between training workloads, game workloads, and match performance in an elite netball team. Methods: Ten elite female netball athletes were monitored over a complete season. Training and game external workloads were determined through inertial movement units and expressed as absolute PlayerLoad (PL) and change of direction (COD). Monthly workload and training efficiency index were also calculated, which used internal workloads (session rating of perceived exertion and summated heart-rate zones). Game performance was assessed through a performance analysis statistic algorithm called NetPoints. To account for the influence of team game workloads on each other, the average workload for midcourt positions (avgMC) was calculated for each game. Data for each athlete were transformed into z scores, and linear mixed modeling was used to build models to examine the relationships between workloads and game performance. Results: Monthly PL, training efficiency index PL, and avgMC PL were statistically significant (P <.05) and positively related to game PL (z = 0.20–0.35, P <.001–.02). For game COD, statistically significant positive relationships were found between monthly COD (z = 0.29 [0.11], P =.01) and avgMC COD (z = 0.21 [0.09], P =.03). The models for NetPoints found significant negative relationships with monthly PL (z = 0.46 [0.12], P <.001) and COD (z = −0.36 [0.11], P =.01). Conclusions: Higher monthly workloads are related to higher game workload; however, they are also related to decreases in match performance. Therefore, netball practitioners should consider that increases to training workload in a 4-week period prior to a game can influence game workloads and performance. [ABSTRACT FROM AUTHOR]

Purpose: Periods away from training and competition are necessary for physical and mental restoration in sport. There is limited research investigating changes to physical qualities in rugby league following prolonged breaks. This study aimed to evaluate the effects of the off-season on muscular power in rugby league. In addition, this study aimed to determine whether the type and volume of training players chose to complete had any relationship to feelings of restoration and/or readiness to return to training. Methods: Twenty professional rugby league players participated in this study. Lower-body muscular power was evaluated using a countermovement jump prior to the off-season and at the recommencement of training. Players completed a questionnaire to identify training and activities completed during the break and to assess feelings of restoration and readiness to commence training. Linear regression was used to estimate the effects of the number of days off on muscular power. One-way repeated-measures analysis of variance was conducted to examine differences in lower-body power throughout the study. Spearman rank order correlation was calculated to determine the relationship between off-season activities and feelings of restoration and readiness. Results: Peak velocity and peak force significantly increased following the off-season break. There were significant relationships between mental restoration and upper-body resistance training, as well as physical restoration and full-body resistance training. Conclusions: The off-season has a positive effect on a player's ability to generate muscular power. Coaches may want to encourage players to complete resistance training sessions with the aim simply to maintain training load and potentially help players to feel rested. [ABSTRACT FROM AUTHOR]