1. Maternal ingestion of cocoa causes constriction of fetal ductus arteriosus in rats
- Author
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Paulo Zielinsky, Felipe Villa Martignoni, Melissa Markoski, Kelly Pozzer Zucatti, Gabriela dos Santos Marinho, Gabriela Pozzobon, Pedro Rafael Magno, Victória de Bittencourt Antunes, Natassia Miranda Sulis, Alexandra Cardoso, Daniel Mattos, Alexandre Antônio Naujorks, Anize Delfino von Frankenberg, and Izabele Vian
- Subjects
Medicine ,Science - Abstract
Abstract Maternal consumption of polyphenol-rich foods has been associated with fetal ductus arteriosus constriction (DAC), but safety of chocolate exposure in fetal life has not been studied. This experimental study tested the hypothesis that maternal cocoa consumption in late pregnancy causes fetal DAC, with possible associated antioxidant effects. Pregnant Wistar rats, at the 21st gestational day, received by orogastric tube cocoa (720 mg/Kg) for 12 h, indomethacin (10 mg/Kg), for 8 h, or only water, before cesaren section. Immediately after withdrawal, every thorax was obtained and tissues were fixed and stained for histological analysis. The ratio of the narrowest part of the pulmonary artery to the fetal ductus inner diameter and increased ductal inner wall thickness characterized ductal constriction. Substances reactive to thiobarbituric acid were quantified. Statistical analysis used ANOVA and Tukey test. Cocoa (n = 33) and indomethacin (n = 7) reduced fetal internal ductus diameter when compared to control (water, n = 25) (p
- Published
- 2021
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