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220 results on '"Kerdjoudj H"'

29. Multiphoton microscopy for blood vessel imaging: new non-invasive tools (Spectral, SHG, FLIM)

Author

Werkmeister, E., Kerdjoudj, H., Marchal, L., Stoltz, J. F., Dumas, D., Dumas, Dominique, Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire (PTIBC-IBISA Nancy), Université Henri Poincaré - Nancy 1 (UHP), Laboratoire Énergies et Mécanique Théorique et Appliquée (LEMTA ), and Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.IB] Life Sciences [q-bio]/Bioengineering, MESH: Humans, Infrared Rays, MESH: Blood Vessels, MESH: Imaging, Three-Dimensional, Imaging, Three-Dimensional, Microscopy, Fluorescence, Multiphoton, MESH: Microscopy, Fluorescence, Multiphoton, Animals, Blood Vessels, Humans, MESH: Animals, [SDV.IB]Life Sciences [q-bio]/Bioengineering, MESH: Infrared Rays
Abstract

International audience; Imaging thick and opaque tissue, like blood vessel, in a noninvasive mode with high resolution, is nowadays possible with multiphoton technology. A near-infrared excitation presents the advantage to be compatible with living specimens and allows a deep penetration into tissues. The nonlinear excitation process is followed by several deactivation ways, among which fluorescence emission can be represented with Spectral or Lifetime imaging. Applied to ex vivo blood vessel imaging, these techniques enabled us to discriminate cell structures (nucleus, cytoskeleton) by fluorescent labelling (Hoechst, QDots). Another method, based on 2-photon excitation and which doesn't need any exogenous dye has also been experimented on arteries: SHG (Second Harmonic Generation) is a diffusion process generated from organized structures. Collagen molecules give rise to a strong SHG signal, enabling us to image the arterial wall (3-dimensional extracellular matrix).

Published
2007

30. Decellularized umbilical artery treated with thin polyelectrolyte multilayer films: potential use in vascular engineering

Author

Kerdjoudj, H., Boura, C., Marchal, L., Dumas, D., Schaff, P., Voegel, J.-C., Stoltz, J.-F., Menu, P., Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire (PTIBC-IBISA Nancy), Université Henri Poincaré - Nancy 1 (UHP), Laboratoire Énergies et Mécanique Théorique et Appliquée (LEMTA ), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), and Dumas, Dominique

Subjects
Umbilical Veins, MESH: Microscopy, Electron, Scanning, Surface Properties, MESH: Trypsin, MESH: Umbilical Veins, Cardiovascular System, MESH: Tissue Engineering, Umbilical Arteries, MESH: Cell Adhesion, Electrolytes, MESH: Electrolytes, Cell Adhesion, MESH: Cryopreservation, Humans, MESH: Microscopy, Confocal, Trypsin, MESH: Arteries, [SDV.IB] Life Sciences [q-bio]/Bioengineering, Cryopreservation, MESH: Surface Properties, Microscopy, Confocal, MESH: Humans, Tissue Engineering, MESH: Cardiovascular System, MESH: Models, Chemical, Arteries, Models, Chemical, MESH: Umbilical Arteries, Microscopy, Electron, Scanning, [SDV.IB]Life Sciences [q-bio]/Bioengineering
Abstract

International audience; Decellularized allograft tissues have been identified as a potential extracellular matrix scaffold for tissue-engineered vascular substitutes. In order to improve the thromboresistance, it is necessary to pre-coat the intra-luminal vessel surface. Recently a new surface modification technique appeared, based on the alternate adsorption of positive and negative charged polyelectrolytes. Our objective was to develop an alternative vascular scaffold made of decellularized human umbilical arteries treated with a PAH/PSS polyelectrolyte multilayered film. The vessels luminal surfaces covered with the multilayer film were observed by electronic scanning microscopy. Our observations showed that the luminal surface is completely devoid of ECs following treatment with trypsin. A top view of the coated artery indicated that the multilayer uniformly covered internal surface of the vessels. The successful of the multilayer correct deposition and retention on the arterial wall were controlled by confocal microscopy using a fluorescent polyelectrolyte (rhodamine-PAH). The data suggest that decellularized cryopreserved arteries represent a potential scaffold for further vascular tissue engineering efforts. Moreover, the multilayer films can be used to coat biological surfaces and following the terminated layer (PAH or PSS), favour the cell adhesion or cell resistance.

Published
2006

32. Gene screening of Wharton's jelly derived stem cells

Author

Mechiche Alami, S., primary, Velard, F., additional, Draux, F., additional, Siu Paredes, F., additional, Josse, J., additional, Lemaire, F., additional, Gangloff, S.C., additional, Graesslin, O., additional, Laurent-Maquin, D., additional, and Kerdjoudj, H., additional

Published
2014
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33. Self‐Defensive Biomaterial Coating Against Bacteria and Yeasts: Polysaccharide Multilayer Film with Embedded Antimicrobial Peptide

Author

Cado, G., primary, Aslam, R., additional, Séon, L., additional, Garnier, T., additional, Fabre, R., additional, Parat, A., additional, Chassepot, A., additional, Voegel, J.‐C., additional, Senger, B., additional, Schneider, F., additional, Frère, Y., additional, Jierry, L., additional, Schaaf, P., additional, Kerdjoudj, H., additional, Metz‐Boutigue, M.‐H., additional, and Boulmedais, F., additional

Published
2013
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34. Evaluating molecular weight of PVP on characteristics of CTA membrane dialysis.

Author

Arous, O., Kerdjoudj, H., Amara, M., and Bensaadi, S.

Subjects
POVIDONE, FOURIER transform infrared spectroscopy, ARTIFICIAL membranes
Abstract

The polymer inclusion membranes used for the selective transport and separation of metal species have emerged in recent years. Their development depends on the method of preparation and the study of their intimate structure. In this work, we have developed a novel class of cellulose triacetate (CTA) membranes modified by poly-electrolytes incorporation that are selectively permeable to metallic ions. The effect of molecular weight of Polyvinylpyrrolidone (PVP) as additive on morphology and transport performance of (CTA) membrane was studied in this work. Diverse CTA membranes have been developed. PVP at different molecular weight (10,000, 24,000 and 40,000) was noted, respectively, (PVPk10, PVPk25 and PVPk30) and Polyethylene glycol (PEG (2000)) were used to modify the morphology and performance of membranes based CTA. The membranes have been prepared by dissolving amounts of CTA, PVP and PEG in Chloroform. The films obtained after solvent evaporation were characterized by thermogravimetric analysis (TGA), Fourier Transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). It was found that the degradation of the membranes is done in two steps. The first one starts at 250 °C due to thermal degradation of the polymeric chains. The SEM image shows that the porosity decreases with increasing of molecular weight of PVP. The influence of the molecular weight of PVP in performance of membranes was examined by application of dialysis process using cations solution containing Cd 2+ , Cu 2+ , Ni 2+ and Zn 2+ . The membrane containing PVPk10 showed better efficiency compared to the other ones. [ABSTRACT FROM AUTHOR]

Published
2016
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50. Self-Defensive Biomaterial Coating Against Bacteria and Yeasts: Polysaccharide Multilayer Film with Embedded Antimicrobial Peptide.

Author

Cado, G., Aslam, R., Séon, L., Garnier, T., Fabre, R., Parat, A., Chassepot, A., Voegel, J.‐C., Senger, B., Schneider, F., Frère, Y., Jierry, L., Schaaf, P., Kerdjoudj, H., Metz‐Boutigue, M.‐H., and Boulmedais, F.

Subjects
COLONIZATION (Ecology), ARTIFICIAL implants, BIOFILMS, BIODEGRADATION, ANTIMICROBIAL peptides, CELL adhesion, PREVENTION
Abstract

Prevention of pathogen colonization of medical implants is a major medical and financial issue since infection by microorganisms constitutes one of the most serious complications after surgery or critical care. Immobilization of antimicrobial molecules on biomaterials surfaces is an efficient approach to prevent biofilm formation. Herein, the first self-defensive coating against both bacteria and yeasts is reported, where the release of the antimicrobial peptide is triggered by enzymatic degradation of the film due to the pathogens themselves. Biocompatible and biodegradable polysaccharide multilayer films based on functionalized hyaluronic acid by cateslytin (CTL), an endogenous host-defensive antimicrobial peptide, and chitosan (HA-CTL-C/CHI) are deposited on a planar surface with the aim of designing both antibacterial and antifungal coating. After 24 h of incubation, HA-CTL-C/CHI films fully inhibit the development of Gram-positive Staphylococcus aureus bacteria and Candida albicans yeasts, which are common and virulent pathogens agents encountered in care-associated diseases. Hyaluronidase, secreted by the pathogens, leads to the film degradation and the antimicrobial action of the peptide. Furthermore, the limited fibroblasts adhesion, without cytotoxicity, on HA-CTL-C/CHI films highlights a medically relevant application to prevent infections on catheters or tracheal tubes where fibrous tissue encapsulation is undesirable. [ABSTRACT FROM AUTHOR]

Published
2014
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