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Low dietary energy and decreased intake of herbage have been attributed to the reduced performance of grazing dairy cattle. We hypothesized that grasses with inherently greater energy would interact in a complementary way with condensed tannins (CT) in birdsfoot trefoil to increase herbage intake by grazing dairy heifers. Eight pasture treatments comprising high-sugar perennial ryegrass (Lolium perenne L.), orchardgrass (Dactylis glomerata L.), meadow bromegrass (Bromus riparius Rehmann), and tall fescue [Schendonorus arundinaceus (Schreb.) Dumort] were established in Lewiston, Utah as monocultures and binary mixtures with birdsfoot trefoil (Lotus corniculatus L.; BFT). Pasture treatments were rotationally stocked by Jersey heifers for 105 d in 2017 and 2018, and herbage samples were collected pre- and postgrazing for each 7-d grazing period and analyzed for herbage mass, nutritive value, and apparent herbage intake. We observed differences among pasture treatments in herbage quantity and nutritive value, as well as differences in herbage intake by grazing Jersey heifers. On average, grass-BFT mixtures had greater herbage intake than grass monocultures, and every grass-BFT treatment individually had greater herbage intake than their respective grass monocultures. Using multivariate analyses, we determined that approximately 50% of the variation in herbage intake was due to nutritive and physical herbage characteristics, with the most explanatory being characteristics related to fiber and energy, followed by those related to the percent of BFT in the herbage. Grass monocultures exhibited a range of inherent dietary energy, but there was indication that an imbalance of energy to crude protein (e.g., protein deficient) reduced intake of grass monocultures. Moreover, there was some evidence of a complementary effect between increased dietary energy and CT; however, low CT levels made it impossible to determine the effect of CT on herbage intake per se. This study confirmed that chemical and physical characteristics inherent to different pasture species have a large effect on herbage intake by grazing cattle. Pastures planted to binary mixtures of nutritious grasses and birdsfoot trefoil increase herbage intake of temperate pastures by grazing Jersey heifers.

Dairy heifers developed in certified organic programs, especially those utilizing pasture-based management schemes, have lower rates of gain than heifers raised in nonorganic confinement production systems in temperate climates, such as in the Intermountain West region of the United States. This study investigates the effects that different forages in a rotational grazing system have on development of organically raised Jersey heifers. Over 3 years, 210 yearling Jersey heifers were randomly assigned to one of 9 treatments, including a conventional confinement control where animals were fed a total mixed ration or one of 8 pasture treatments: Cache Meadow bromegrass (Bromus riparius Rehmann), QuickDraw orchard grass (Dactylis glomerata L.), Amazon perennial ryegrass (Lolium perenne L.), or Fawn tall fescue (Schendonorus arundinaceus [Schreb.] Dumort) and each individual grass interseeded with birdsfoot trefoil (Lotus corniculatus L., BFT). Each treatment had 3 blocks/yr over the 3-yr period, with each block having a 0.4 ha pasture of each treatment. Every 35 d, over a 105-d period, heifers were weighed and measured for hip height, and blood samples were collected to determine serum insulin-like growth factor-1 and blood urea nitrogen concentrations. Fecal egg counts were also assessed. Heifer body weight (BW), blood urea nitrogen, and insulin-like growth factor-1 concentrations were affected by treatment when analyzed over time. Heifers on grass-BFT pastures had increased BW compared with heifers on monoculture grass pastures. Heifers receiving a total mixed ration or perennial ryegrass+BFT had increased BW gain over the 105-d period compared with heifers grazing tall fescue+BFT, orchard grass, perennial ryegrass, meadow bromegrass, or tall fescue. Individually for all grass species, heifers grazing +BFT pastures had greater ending BW and weight gain than heifers grazing the respective grass monocultures. Furthermore, weight gain for heifers on perennial ryegrass+BFT, meadow bromegrass+BFT, and orchard grass+BFT were not different from those on a total mixed ration. Heifers grazing grass-BFT pastures had increased blood urea nitrogen compared with heifers grazing monoculture grass pastures. Heifer hip height and fecal egg counts were not affected by treatment. These results show that the addition of BFT to organic pasture improves growth of grazing replacement heifers. Economic analyses also demonstrate that interseeding grass pastures with BFT results in an increased economic return compared with grazing monoculture grass pastures. Grass pastures interseeded with BFT may be a sustainable option to achieve adequate growth of Jersey heifers raised in an organic pasture scenario in a temperate climate.

Simple Summary Alternatives to antibiotic therapy for mastitis in dairy cattle are of interest to the dairy industry and society. Reduced use of antibiotics while maintaining or improving animal welfare is desirable. We studied the intramammary infusion of casein hydrolysate, alone or combined with standard dry cow treatment, at the beginning of the dry period before cows have their next calf. The effects on mammary involution and milk quality suggested that infusion of casein hydrolysate alone or combined with internal teat sealant may be an alternative to antibiotic dry cow therapy. Abstract Alternatives to routine antibiotic treatment of dairy cattle during the dry period before their next calving are of interest. This was a preliminary study of whether intramammary infusion of casein hydrolysate, administered alone or combined with standard dry treatment, accelerated the rate of mammary involution early in the dry period. Four treatments were studied in a split udder design. One udder half was assigned a treatment, and the contralateral half was administered dry cow treatment + internal teat sealant as a control. Treatments were casein hydrolysate, casein hydrolysate + dry cow treatment, casein hydrolysate + teat sealant and casein hydrolysate + dry cow treatment + teat sealant. Cows (n = 16) were blocked by a number of intramammary infections per udder half (0 or 1+) and randomized to treatments. Milk production was not different between control or treated udder halves post-calving. A generalized linear mixed model tested for differences between the treatment groups in the concentration of mammary involution indicators in milk: somatic cell count, bovine lactoferrin and bovine serum albumin. At 7 days, dry udder halves treated with casein hydrolysate had higher milk concentrations of lactoferrin than those treated with casein hydrolysate + dry cow treatment, casein hydrolysate + teat sealant and control. At 10 days dry, bovine serum albumin was higher in udder halves treated with casein hydrolysate than in those treated with casein hydrolysate + dry cow treatment, casein hydrolysate + dry cow treatment + teat sealant and control. Post-calving, casein hydrolysate-treated udder halves produced 51% of total milk, unchanged from before dry-off. There were seven total intramammary infections entering the dry period, all caused by coagulase-negative staphylococci. Cure rates (3/7, 43%) were not different among all treatments and control, partly because of the small sample size. Intramammary infusion of casein hydrolysate at the end of lactation may be an alternative or possible adjunct to antibiotic dry cow therapy.

The breadth of animal hosts that are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and may serve as reservoirs for continued viral transmission are not known entirely. In August 2020, an outbreak of SARS-CoV-2 occurred in multiple mink farms in Utah and was associated with high mink mortality and rapid viral transmission between animals. The outbreak’s epidemiology, pathology, molecular characterization, and tissue distribution of virus within infected mink is provided. Infection of mink was likely by reverse zoonosis. Once established, infection spread rapidly between independently housed animals and farms, and caused severe respiratory disease and death. Clinical signs were most notably sudden death, anorexia, and increased respiratory effort. Gross pathology examination revealed severe pulmonary congestion and edema. Microscopically there was pulmonary edema with moderate vasculitis, perivasculitis, and fibrinous interstitial pneumonia. Reverse transcriptase polymerase chain reaction (RT-PCR) of tissues collected at necropsy demonstrated the presence of SARS-CoV-2 viral RNA in multiple organs including nasal turbinates, lung, tracheobronchial lymph node, epithelial surfaces, and others. Whole genome sequencing from multiple mink was consistent with published SARS-CoV-2 genomes with few polymorphisms. The Utah mink SARS-CoV-2 strain fell into Clade GH, which is unique among mink and other animal strains sequenced to date and did not share other spike RBD mutations Y453F and F486L found in mink. Localization of viral RNA by in situ hybridization revealed a more localized infection, particularly of the upper respiratory tract. Mink in the outbreak reported herein had high levels of virus in the upper respiratory tract associated with mink-to-mink transmission in a confined housing environment and were particularly susceptible to disease and death due to SARS-CoV-2 infection.Author SummaryThe recent emergence and worldwide spread of the novel coronavirus has resulted in worldwide disease and economic hardship. The virus, known as SARS-CoV-2 is believed to have originated in bats and has spread worldwide through human-to-human virus transmission. It remains unclear which animal species, other than humans, may also be susceptible to viral infection and could naturally transmit the virus to susceptible hosts. In this study, we describe an outbreak of disease and death due to SARS-CoV-2 infection in farmed mink in Utah, United States. The investigation reveals that mink can spread the virus rapidly between animals and that the disease in mink is due to the viral infection and damage to tissues of the upper and lower respiratory system. The determination that mink are susceptible to SARS-CoV-2 indicates the need for strict biosecurity measures on mink farms to remediate mink-to-mink and human-to-mink transmission for the protection of mink, as well as prevent potential transmission from mink to humans.

Wild parsnip (Pastinaca sativa) has been associated with livestock and human photosensitization. An investigation of a natural occurrence of photosensitization of grazing horses identified wild parsnip as a possible cause. HPLC-MS and MS/MS analysis of this plant identified five furanocoumarins i.e., xanthotoxin, bergapten, isopimpinellin, imperatorin and a putative methoxyimperatorin. Goats fed this wild parsnip were largely unaffected. Xanthotoxin was not detected in the serum of parsnip-fed goats or in the serum of goats dosed orally or intravenous with purified xanthotoxin. Cutaneous application produced severe photodermatitis in goats and a horse consistent with topical exposure as the likely route to produce wild parsnip-induced photosensitivity. Wild parsnip-induced superficial necrotizing dermatitis was consistent with photodermatitis with no evidence of other allergic or inflammatory components.

The breadth of animal hosts that are susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and may serve as reservoirs for continued viral transmission are not known entirely. In August 2020, an outbreak of SARS-CoV-2 occurred on five mink farms in Utah and was associated with high mink mortality (35–55% of adult mink) and rapid viral transmission between animals. The premise and clinical disease information, pathology, molecular characterization, and tissue distribution of virus within infected mink during the early phase of the outbreak are provided. Infection spread rapidly between independently housed animals and farms, and caused severe respiratory disease and death. Disease indicators were most notably sudden death, anorexia, and increased respiratory effort. Gross pathology examination revealed severe pulmonary congestion and edema. Microscopically there was pulmonary edema with moderate vasculitis, perivasculitis, and fibrinous interstitial pneumonia. Reverse transcriptase polymerase chain reaction (RT-PCR) of tissues collected at necropsy demonstrated the presence of SARS-CoV-2 viral RNA in multiple organs including nasal turbinates, lung, tracheobronchial lymph node, epithelial surfaces, and others. Localization of viral RNA by in situ hybridization revealed a more localized infection, particularly of the upper respiratory tract. Whole genome sequencing from multiple mink was consistent with published SARS-CoV-2 genomes with few polymorphisms. The Utah mink SARS-CoV-2 strains fell into Clade GH, which is unique among mink and other animal strains sequenced to date. While sharing the N501T mutation which is common in mink, the Utah strains did not share other spike RBD mutations Y453F and F486L found in nearly all mink from the United States. Mink in the outbreak reported herein had high levels of SARS-CoV-2 in the upper respiratory tract associated with symptomatic respiratory disease and death., Author summary The recent emergence and spread of the novel coronavirus has resulted in worldwide disease and economic hardship. The virus, known as SARS-CoV-2 is believed to have originated in bats and has spread worldwide through human-to-human virus transmission. It remains unclear which animal species, other than humans, may also be susceptible to viral infection and could naturally transmit the virus to susceptible hosts. In this study, we describe the early phases of an outbreak of disease and death due to SARS-CoV-2 infection in farmed mink in Utah, United States. The investigation reveals that mink can spread the virus rapidly between animals and that the disease in mink is associated with viral infection and damage to tissues of the upper and lower respiratory system. The determination that mink are susceptible to SARS-CoV-2 indicates the need for strict biosecurity measures on mink farms to remediate mink-to-mink and human-to-mink transmission for the protection of mink, as well as prevent potential transmission from mink to humans. It may be prudent for humans working with SARS-CoV-2 mink to wear appropriate PPE until more is understood.

This research analyzed how maternal plane of nutrition during mid-gestation impacts growth, blood metabolites, expression of microRNA and messenger RNA in skeletal muscle, feedlot performance, and carcass characteristics of progeny. Thirty-two cows were bred to the same Angus sire and fed to either maintain a body condition score (BCS) of 5.0 to 5.5 (maintenance [MAIN]; n = 15) or to lose 1 BCS (restriction [REST]; n = 17) over an 84-d period of mid-gestation. Following the second trimester, all cows were co-mingled and fed at maintenance for the remainder of gestation. Following the 84-d treatment period, REST cows had a lower (P < 0.01) BCS than MAIN cows. At the end of the third trimester, there was no difference (P = 0.78) in BCS between the treatment groups. There was no difference (P > 0.10) between offspring in birthweight, weaning weight, average daily gain, feed efficiency, dry matter intake, carcass yield, steak quality, or in circulating levels of glucose, cortisol, insulin, or insulin-like growth factor-1. REST offspring expressed more (P < 0.05) miR-133a, miR-133b, miR-181d, miR-214, miR-424 and miR-486 at weaning than MAIN offspring. At harvest, REST offspring expressed more (P < 0.05) miR-133a and less (P < 0.01) miR-486 than MAIN offspring. REST steaks were perceived as more tender (P = 0.05) by a trained sensory panel. These results indicate that maternal nutrient restriction during mid-gestation resulting in a loss of 1 BCS has an effect on microRNA expression in the skeletal muscle but does not alter postnatal growth potential, carcass quality, or end product quality of the offspring. This suggests that moderate restriction in maternal nutrition during the second trimester, which results in a drop in BCS that can be recovered during the third trimester, should not cause alarm for producers when considering future offspring performance.

This study investigates the effects that different forages in a rotational grazing system have on pH and VFA proportions of rumen fluid in organically raised dairy heifers. In 2018, 81 yearling Jersey heifers were assigned to one of nine treatments, including a conventional dry lot control (TMR) or one of eight pasture treatments. Pasture treatments included: tall fescue (TF), meadow bromegrass (MB), orchard grass (OG), perennial ryegrass (PR) and each individual grass interseeded with birdsfoot trefoil (BFT). Every 35-d, over a 105-d period, rumen fluid was collected using an oral stomach tube. Immediately following collection, pH was measured and recorded. Rumen fluid collected for VFA analysis was stabilized with acid and placed on wet ice and eventually frozen at -80° C until further analysis. Repeated measures analyses were performed using PROC MIXED in SAS (version 9.4) to determine how treatment affected each measurement over the total 105-day period. Time had a significant effect (P < 0.001) on pH ofrumen fluid over the 105-d growth period. Additionally, treatment had a significant effect (P < 0.001) on pH of rumen over the 105-d growth period. Animals that consumed the TMR diet had decreased rumen pH compared to other treatments. The proportions of acetate, propionate, isobutyrate, butyrate, isovalerate, and valerate were all significantly affected (P < 0.001) by time over the 105-d growth period. Treatment had an effect (P < 0.05) on the proportions ofpropionate and butyrate and had a tendency (P = 0.06) to affect the proportion of isovalerate. In general, animals on the TF pastures had decreased (P < 0.05) proportions of these VFAs in the rumen fluid. The proportions of acetate, isobutyrate, and valerate were not affected (P < 0.05) by treatment. These data provide insight into how different forages in organic pastures affect rumen characteristics of growing Jersey heifers.

Many breeds of dairy cattle exist, but questions remain about their relative performance within exclusively forage-based systems in the United States. This paucity of data is especially apparent in relation to heifer development, which is the second largest expense on most dairy farms. We evaluated the performance of prepubertal heifers from four different genetic backgrounds (“breeds”) within a rotational grazing system in the Intermountain West. For each of two 105-day grazing seasons, 24 dairy heifers from each of four different breeds [Holstein (HO), Jersey (JE), Holstein/Jersey crossbred (HJ), and Swedish Red/Holstein/Montbeliarde crossbred (SHM)] were randomly assigned to one of two pasture treatments that consisted of either grass only (MONO) or grass interseeded with the legume birdsfoot trefoil (BFT; MIX). Main effects of treatment and breed (and potential interactions) on heifer average daily gain (ADG), change in percent mature body weight (CPMBW), fecal egg count (FEC), rumen volatile fatty acids (VFA), and body condition scores (BCS) were evaluated. There was a significant effect of pasture treatment on ADG, with MIX heifers averaging 0.48 kg of gain/day, whereas MONO heifers averaged 0.29 kg/day (P < 0.0001). Change in BCS was significantly affected by treatment (P = 0.0012) and breed (P < 0.0001). Significant effects of treatment (P < 0.0001), breed (P = 0.0005), and treatment*breed (P = 0.0005) on CPMBW were also found. Treatment and breed had no effect on FEC or total rumen VFA content (P > 0.05). Overall, we found that Jerseys were able to gain a higher percentage of their mature body weight and lose less body condition while on pasture than heifers of other breeds. We also found that grazing BFT can have significant positive effects on ADG, BCS, and CPMBW. Further research is needed to more thoroughly evaluate the influence of heifer genetics (breed) on metrics of growth, health, reproductive performance, and production in pasture settings.

Mineral deficient cattle that enter a feedlot have been documented to have increased morbidity and mortality rates and decreased feedlot performance. The objective of this research was to explore current practices used by feedlot producers when receiving mineral deficient cattle. To accomplish this, forty steers were blocked by weight and mineral status, then assigned to one of four treatment groups: control (Con; n = 10), industry mineral requirements (IND; n = 10) top dressed on TMR fed, 2X industry mineral requirements (2XIND; n = 10) top dressed on TMR fed, and MultiMin® injection at labeled dose (MM; n = 10). All steers received the same ration in pens equipped with GrowSafe® bunks. Standing liver biopsies were collected on day 0, 5, 10, 20, 30 and 40 and analyzed for mineral content. Steers were weighed on day 0, 14, 28, 41, 56, 70, 84, and 110. Average daily gain (ADG), dry matter intake (DMI) and feed efficiency (FE) were recorded. After the trial, steers were harvested at a commercial facility and carcass data was obtained from the plant. There were no differences (P > 0.05) in weight gain or overall ADG between treatment groups. When analyzed over time, treatment had an effect (P < 0.001) on liver copper and selenium concentrations, but no effects (P > 0.05) were observed on liver manganese and zinc concentrations. Additionally, when DMI was analyzed over time, effects (P < 0.05) of time, treatment, and treatment*time were observed, and effects (P < 0.05) of time and treatment*time were observed on FE. Treatment had no effect (P > 0.05) on carcass data. These data indicate that different mineral supplementation strategies have diverse effects on feedlot performance and liver mineral concentrations over time.

Practicing veterinarians are exposed to unique occupational hazards and zoonotic diseases. National studies have highlighted an increase risk to injuries and a lack of veterinary awareness for these hazards. In Utah (and likely other states), reports of acquired zoonoses are sporadic, and underlying risk factors poorly understood. To better clarify occupational risk factors, the knowledge, attitudes, and behaviors of Utah veterinarians were examined.A total sample of 809 Utah veterinarians were identified from the 2017 licensure listing provided by the Utah Division of Professional Licensing. These individuals were invited to participate in an internet-based survey to self-report musculoskeletal injuries associated with large animal palpation practices. The relationships between some variables and Precaution awareness (PA) score rankings were examined using logistic regression and odds ratios.Of 809 veterinarians, 223 (28%) responded, with 209 self-identifying as clinical veterinarians. Frequent palpation of large animals (equine or bovine) was performed by 43 (22%) of 197 responding veterinarians. Joint pain or injury was experienced by 29 (67%) of 43 responding veterinarians who frequently palpated large animals (equine or bovine). Animal bites, needle-sticks, and cuts were the most frequently reported injuries within the past year. There were 44 (22%) of 197 respondents who reported having contracted a zoonotic disease. Most of the respondents failed to use appropriate PPE when examining an ill animal or when handling potentially infectious specimens. Clinical veterinarians who have less than 10 years of experience and work in a practice providing clinic-based services were 2.3 and 3.8 times more likely to have low PA rankings, respectively.Our work underscores the importance for continued clinical veterinarian education on occupational risks associated with musculoskeletal injuries, animal bites, needle-sticks, and cuts. The fact that survey veterinarians reported a high level of zoonotic disease infection illustrates the need for education and training on proper PPE use. Further work is needed to understand why precaution awareness scores were low among this sample of participants in this study. Additional follow-up with veterinarians is recommended to assess availability of large animal handling facilities and safety practices to prevent musculoskeletal injuries.

Cows nutritionally restricted during mid-gestation produce calves with differential fat and muscle deposition when compared to calves from non-restricted cows. However, little is currently known about effects these changes have on post-natal skeletal growth. We investigated whether calves from cows restricted in the second trimester had different expression of microRNA (miR), messengerRNA, and muscle fiber type when compared to calves from non-restricted dams. Cows were bred by the same sire, stratified by body weight (P = 0.80) and allocated to one of two treatments: maintenance (n = 16) or restricted (n = 18). Restricted cows received lower forage biomass (1662 kg/ha, dry matter (DM)) compared to maintenance (2309 kg/ha, DM). Restricted cows had mean BCS 1.55 lower (P = 0.001) than maintenance cows and weight difference of 188 kg (P = 0.024). After the second trimester, all cows and calves were treated similarly. Skeletal muscle biopsies were collected from calves at weaning, beginning of the feedlot, and harvest. Offspring from restricted cows expressed more (P < 0.05) miR-133a, -133b, -181d, -214, -424 and -486 in the biceps femoris (BF) at the beginning of the feedlot when compared to maintenance offspring. At harvest, offspring from restricted cows expressed more (P < 0.05) miR-133a and less (P < 0.01) miR-486 in the longissimus lumborum (LD) than offspring from maintenance cows. There was no change (P ≥ 0.27) in expression of Pax3, Pax7, Cdc25A, MamL1, Ezh2, and IGF-1R between offspring from the two treatment groups in the BF at the beginning of the feedlot or the LD at harvest. Expression was not different (P ≥ 0.14) in myosin heavy chain isoforms MHC-1, -2a, and -2x between offspring from maintenance and restricted in the BF at the beginning of the feedlot or the LD at harvest. These data provide novel insight into the molecular mechanism through which nutrient restriction in the second trimester alters fat and muscle deposition in the offspring.

Reduced developmental competence after IVF has been reported using oocyte derived from small follicles in several species including cattle, sheep, and goats. No information is currently available about the effect of follicle size of the cytoplast donor on in vivo development after somatic cell nuclear transfer (SCNT) in goats. Oocytes collected from large (≥3 mm) and small follicles (

We investigated how nutritional restriction of cows during the second trimester of gestation impacted the muscle fiber type proportions of offspring at 10 months after birth. Cows were bred by the same sire, stratified by body weight (P = 0.80) and body condition score (BCS) (P = 0.72), and allocated to one of two treatment groups during the second trimester of gestation: maintenance (n = 16, 2309 kg/ha dry matter (DM)) or restricted (n = 18, 1662 kg/ha DM). Restricted cows had a BCS 1.55 lower (P = 0.001) than maintenance cows at the end of the period and a weight difference of 188 kg (P = 0.024). After the second trimester, all cows were fed similarly and there was no difference (P > 0.80) in body weight or BCS between the dams from either treatment at the end of the third trimester. There were no differences (P > 0.05) in body weight of offspring at birth, throughout growth, or during the feedlot. Skeletal muscle biopsies were collected from the biceps femoris of offspring at 10 months of age and stained using both a succinate dehydrogenase stain and myosin adenosine triphosphate, There were no differences (P > 0.15) in proportions of type I, IIa or IIb fibers when comparing offspring from maintenance or restricted dams. However, the proportion of type I fibers was negatively correlated (r = -0.48, P = 0.06) with Warner-Bratzler shear force value and type IIa fiber proportions showed a weak, positive correlation (r = 0.37, P = 0.10) with total DM intake of the offspring during the feedlot phase. These preliminary data demonstrate that a nutritional restriction during the second trimester of gestation does not significantly alter fiber type proportions of the offspring prior to entering the feedlot.

Humans have shaped carnivore behavior since at least the Middle Paleolithic period, about 42,000 years ago. In more recent times, spotted hyenas (Crocuta crocuta) in Ethiopia have adapted to living in urban areas, while humans have adapted to living with hyenas. Yet, relationships between coexisting humans and carnivores are rarely addressed beyond mitigating conflicts. We provided a case study for how to broadly think about coexistence and how to study it when measuring if humans and carnivores affect one another. We collected data in four Ethiopian cities: Mekelle, Harar, Addis Ababa, and Arba Minch. We held focus groups and key informant interviews that incorporated feedback from 163 people, representing a wide array of religious, economic, and educational backgrounds. We also determined how many hyenas resided in these cities, hyena behavioral responses to humans using a flight initiation test, and problem-solving abilities via puzzle box trials. We found that in three of the cities, hyenas and humans coexist at high densities and frequently encounter each other. While all participants recognized the importance of hyenas as scavengers to maintain a clean environment, there was pronounced variation in cultural perspectives across cities. For example, while the people of Harar revere hyenas in spiritual terms, in Arba Minch hyenas were regarded as nuisance animals. Hyenas were universally respected as a formidable predator across cities but reports of attacks on livestock and humans were few. Flight initiation tests revealed hyenas fled at significantly closer distances in Harar and Addis Ababa than in Mekelle. Hyenas succeeded at solving a puzzle box in Harar but not in Mekelle. These variable behavior in hyenas correlated to different human perceptions. Our case study results suggest that the hyena&ndash, human dynamic is highly variable across these locations. We conclude by exploring the implications of these findings for how humans and hyenas can shape one another&rsquo, s behavior. Developing studies to link human perceptions and animal behavior could advance wildlife conservation, especially in urban areas.

Mastitis in a single quarter can cause high somatic cell counts (SCC), clinical mastitis, and death in dairy cows. Currently, management of these mastitic quarters presents a problem for the dairy industry. Casein hydrolysate (CH) is an intramammary (IMM) infusion treatment reported to induce mammary involution. The primary objectives of this study were to investigate whether IMM CH treatment of single high SCC quarters, followed by cessation of quarter milk production for the remainder of lactation, was effective in reducing cow–level SCC and whether that quarter resumed milk production following calving. Three treatment groups were used: CH, non-hydrolyzed casein (NHC), and cessation of milking only (negative; N). Treatments were assigned in a 2:2:1 ratio for 40 cows enrolled in the study; 27 cows completed the entire protocol. Following IMM infusion and involution of the single mastitic quarter, decreases in cow–level SCC (-966,000/ml) and milk production (-11 lb (5 kg), -14%) with 3 remaining lactating quarters were significant for all 28 cows combined. Cows treated with CH (n=17) had a significant decrease in cow– level SCC (-1,150,000/ml) during remaining lactation. All treated quarters returned to milk production after calving, and their proportion of total–cow milk production (24%) was not different than before treatment (28%). After calving, treated quarters’ decrease in SCC was significant for CH (-2,763,000/ml; n=14) and N (–5,324,000/ml; n=5). Of 16 quarters with positive milk culture before treatment that completed the protocol, 88% (14/16) were cured (no isolation of the same bacteria for 3 weeks following calving). A new intramammary infection (IMI) was detected in 67% (18/27) of previously treated quarters post-calving. Infusing single mastitic quarters with casein hydrolysate to induce involution for the remainder of lactation may be a promising alternative to current methods.

A decrease in the incidence of bovine mastitis, the costliest disease in the dairy industry, can be facilitated through genetic marker-assisted selective breeding programs. Identification of genomic variants associated with mastitis resistance is an ongoing endeavor for which genome-wide association studies (GWAS) using high-density arrays provide a valuable tool. We identified single nucleotide polymorphisms (SNPs) in Holstein dairy cattle associated with mastitis resistance in a GWAS by using a high-density SNP array. Mastitis-resistant (15) and mastitis-susceptible (28) phenotypic extremes were identified from 224 lactating dairy cows on commercial dairy farm located in Utah based on multiple criteria of mastitis resistance over an 8-month period. Twenty-seven quantitative trait loci (QTLs) for mastitis resistance were identified based on 117 SNPs suggestive of genome-wide significance for mastitis resistance (p ≤ 1 × 10−4), including 10 novel QTLs. Seventeen QTLs overlapped previously reported QTLs of traits relevant to mastitis, including four QTLs for teat length. One QTL includes the RAS guanyl-releasing protein 1 gene (RASGRP1), a candidate gene for mastitis resistance. This GWAS identifies 117 candidate SNPs and 27 QTLs for mastitis resistance using a selective genotyping approach, including 10 novel QTLs. Based on overlap with previously identified QTLs, teat length appears to be an important trait in mastitis resistance. RASGRP1, overlapped by one QTL, is a candidate gene for mastitis resistance.

The primary objective was assessment of effect of sample pooling on Tritrichomonas foetus detection using qPCR in beef bulls within 5 logs of trichomonad concentration in spiked preputial samples. The secondary objective was to use qPCR Ct values to estimate the percentage of beef bulls in the field population within each log of trichomonad concentration. Solutions containing none, 1,10,100,1000 and 10,000 trichomonads (T)/ml were made and tested by qPCR. Test sensitivities for single positive samples were: 1 T/ml, 20/40 (50%); 10 T/ml, 40/40 (100%); 100 T/ml, 40/40 (100%); 1000 T/ml, 40/40 (100%); 10,000 T/ml, 40/40 (100%). For 5-sample pools (1 positive and 4 negative), sensitivities were: 1 T/ml, 6/40 (15%), 10 T/ml, 26/40 (65%); 100 T/ml 40/40 (100%); 1000 T/ml, 40/40 (100%); 10,000 T/ml, 40/40 (100%). Specificity for both single and pooled negative samples was 40/40 negative (100%). Diagnostic field samples (n = 130) in which T. foetus was detected by qPCR were classified by Ct to estimate the proportion of positive bulls within each trichomonad concentration: 1 T/ml 10 (7.7%); 10 T/ml 17 (13.1%); ≥ 100 T/ml 103 (79.2%). Accounting for the above sensitivities and proportions of bulls, sensitivity for trichomoniasis detection in the bull population was 96.2% using single preputial samples and 88.9% using 5-sample pools. Therefore 7.3% of all T. foetus-positive bulls were not detected using 5-sample pools compared with using single samples. Detection of bovine trichomoniasis by qPCR is minimally affected by using 5-sample pools, considering the trichomonad concentration of most positive bulls.

Recent outbreaks of Chikungunya virus (CHIKV) infection have resulted in millions of cases of disease with significant morbidity. No approved antiviral treatments exist for the prevention or treatment of this viral disease. Infection with CHIKV results in a high rate of symptomatic disease that primarily includes a debilitating arthralgia. To model this cardinal disease manifestation, adult DBA/1J mice were challenged with CHIKV by footpad injection. Viremia and hind limb virus titers increased ~100-fold while spleen virus increased >1,000-fold within 1 day post-virus infection (dpi). Footpad swelling was measured over a 10-day period, with peak swelling observed between 6 and 7 dpi. Histology of the hind leg at the site of virus challenge showed evidence of myositis and synovitis starting on 5 dpi. Cytokine profiling of the hind limb at the site of inoculation revealed a biphasic inflammatory response represented by an increase in IL-6, MCP-1, IFN-γ, MIP-1α, RANTES, and IL-17. To investigate the prophylactic capacity of IFN, mice were treated with mDEF201, an adenovirus-vectored IFN-α. Intranasal administration of a single 107 pfu/ml dose of mDEF201 administered 21 days to 24 h prior to infection, significantly reduced footpad swelling, virus titers in the hind leg and spleen, and several inflammatory cytokines. Efficacy was not observed when treatment was initiated 24 h after virus challenge. This arthralgia model of CHIKV recapitulates relevant disease features commonly observed in human disease making it applicable to preclinical testing of therapies that target both viral replication and the associated joint disease.

The hypothesis of this study was that the leukocyte populations and expression levels of genes related to immune response, growth factors and apoptosis would be altered at the fetal-maternal interface in somatic cell nuclear transfer (SCNT)-generated sheep pregnancies. Placental and endometrial samples from sheep pregnancies established by SCNT and natural breeding (control) were collected at 45 days and at term. Expression of genes related to growth factors, apoptosis and immune response was examined using quantitative reverse transcription polymerase chain reaction. Endometrial leukocyte populations and major histocompatibility class I (MHC-I) protein expression were examined by immunohistochemistry. At term we observed altered expression of genes related to apoptosis, growth factors and immune response in placental and endometrial tissue of SCNT pregnancies. In Day-45 pregnancies there was less-pronounced abnormal expression and only genes related to apoptosis and growth factors were abnormal in the placenta. Endometrial gene expression profiles were similar to age-matched controls. Placental MHC-I protein expression was similar in SCNT and controls at 45 days but increased in the SCNT at term. The altered gene expression at the fetal-maternal interface likely contributes to the placental dysfunction and overgrowth observed in sheep SCNT pregnancies.

The objectives of this study were to estimate the dairy herd-level prevalence of Johne's disease (JD) in Utah and nearby areas of the intermountain west and to estimate the sensitivity of a single bulk-tank milk test for JD detection. Two milk samples from all bulk tanks on the study farms were collected 1 mo apart. Samples were frozen and shipped to a laboratory for JD testing. An ELISA to measure total IgG antibody specific against Mycobacterium avium ssp. paratuberculosis , the etiological agent that causes JD, and a quantitative real-time PCR to detect M. avium ssp. paratuberculosis DNA were used; both tests were designed for bulk milk. Of the dairy farms in the study area, 170/246 (69%) participated. Positive JD results were found in bulk milk from 67/170 (39%) of dairy farms in Utah and adjacent areas. There were 138 JD-positive bulk-tank results from 241 bulk-tank samples from the 67 positive herds. The sensitivity of the bulk milk testing for detection of JD was 138/241(57%). From the 103 JD-negative farms, 235 bulk-tank samples tested negative for JD. The probability of false-negative results on a single bulk-milk sample was (1 – 0.57)=0.43. For farms with 1 bulk tank, 2 samples collected 1 mo apart, with both samples testing negative (by both ELISA and quantitative real-time PCR) for JD, the true-negative probability was [1 – (0.43) 2 ]=(1 – 0.18)=82%. For farms with at least 2 bulk tanks, at least 4 samples tested, with all results negative for JD, the true-negative probability was at least 97%. Results support other estimates that prevalence of JD has increased over the last 15 to 20 yr. However, the prevalence detected was 3 times that from a recent report where 13% of dairy herds in the western US were positive. The increase in JD suggests that current control programs, at least as applied, are not effective. Bulk milk testing is a practical way to screen dairy herds for presence of JD. Studies are needed regarding the use of individual cow milk tests for accuracy, practicality, and effectiveness in reducing the prevalence of JD in dairy herds.

Herd-level prevalence of Mycobacterium avium subsp. paratuberculosis (MAP), causative agent of Johne’s disease (JD) and Bovine Viral Diarrhea (BVD) virus were estimated on dairy farms in Utah. Duplicate milks were collected at 3-4 day intervals on 5 dates from each bulk tank on participating farms. Samples were tested at separate laboratories for BVD (real-time, RT-PCR) and for JD/MAP (ELISA and qPCR). 151/209 (72%) eligible dairy farms participated. Farms detected positive were: 58 JD (38%) and 14 BVD (9%); 5 farms had both diseases. Follow up visited farms’ (n=22) means, medians: 778,420 milking cows; 20,052 lbs, 20,311 lbs 305d milk; 175,545/ml, 178,000/ml bulk milk SCC; stalls visibly soiled in rear one-third 37%, 32%, range 5% to 90%. Seventeen of 21 (81%) farms with JD had observed adult cows becoming thin while retaining appetite, 52% had seen adult cows contract diarrhea and subsequently die. Both BVD-positive farms had observed abortions. Free stalls housed milking cows on 91% of farms; dry lots housed dry cows on 55%. Nine farms (41%) had purchased animals within the past year: 27% pregnant heifers, 18% bulls, 9% calves, 14% cows; 9% had purchased only bulls. Whenever animals were last purchased, 14 farms (64%) had performed no disease testing or segregation; 8 farms (36%) utilized at least one biosecurity practice for replacements. Most common were 9-way vaccine including BVD on arrival (27%), 14% segregated replacements for any time, 11% tested for any diseases (none for JD). Fourteen (67%) farms with JD would identify known positive cows; none would segregate positives. Most producers (57%) allowed known JDpositive cows to calve again, farms with BVD were equivocal. No producers would have a separate calving area for JD or BVD-positive cows. Six farms (27%) fed calves only individual cow colostrum and pasteurized milk. All 22 farms vaccinated against BVD.

We isolated Corynebacterium pseudotuberculosis from an abscess of the head of a captive elk submitted for necropsy and from a similar abscess of a living herd mate. To our knowledge, this is the first documented case in elk and should be considered a potential cause of subcutaneous abscesses in wild elk.

Dairy herd-level prevalence of Mycobacterium avium subsp. paratuberculosis (MAP), causative agent of Johne’s disease (JD), Mycoplasma spp., and Bovine Viral Diarrhea (BVD) virus were estimated in Utah and surrounding states and compared to previous surveillance results. Milk was collected at 3-4 day intervals on 5 dates (duplicate samples) from each bulk tank on participating farms, samples analyzed separately. One frozen sample was shipped to a laboratory for JD/MAP testing with ELISA and real-time PCR, the other paired sample was shipped to another laboratory for mycoplasma and BVD testing. Mycoplasma was cultured on modified Hayflick medium, standard methods; BVD testing was real-time, RT-PCR. 151/209 (72%) eligible dairy farms participated. Farms detected positive (some had multiple diseases) were: 58 JD (38%); 4 mycoplasma (3%); 14 BVD (9%). Sensitivity of testing was: 284/528 milks = 54% for JD, 17/61 = 28% for mycoplasma (lower than previous reports), 41/117 = 35% for BVD. Of 67 herds positive for JD in 2009, 28 (42%) remained positive, 14 (21%) became test-negative, and 25 (37%) were lost to follow up. Of 16 herds positive for mycoplasma in 2007, one (6%) remained positive, 8 (50%) became testnegative, and 7 (44%) were lost to follow up. Bulk milk remains a practical way to screen dairy herds for presence of JD, mycoplasma and BVD, provided that repeated sampling is used. Mycoplasma-positive herds were more likely to become test-negative in bulk milk in subsequent years than were JD-positive herds. Prevalence of BVD was similar to but slightly lower than previously reported.

A milk ELISA test for Antibody (Ab) against Bovine Viral Diarrhea (BVD) virus was studied in a dairy herd with past diagnoses of calves dying from BVD and Persistently Infected (PI) cows, with culling of all known PI cows. Modified live BVD vaccine was administered to calves 3 months and 4 months old, all cows at dryoff 45 to 60 days before calving, and 15-21 days in milk (DIM). Cows were tested 1 month apart (247 and 258 cows, respectively) using a competitive ELISA for milk Ab binding to p80 BVD non-structural protein. Results are reported as % binding by a second Ab; higher second Ab binding means the milk had less anti-p80 BVD Ab. Cows with 90-100% binding in milk on both tests were classified as low Ab–interpreted as a cow with PI or vaccine failure. Milk handling method was significant; fresh milk mean 49% second Ab binding was higher than for milk preserved 3 other ways. In fresh milk, 15 cows had 90-98% binding on one test, but 14/15 were milking during both herd tests and were below 90% on the other tests. Stage of lactation significantly affected results; anti-BVD Ab was higher from 1-30 DIM and lower from 61-150 DIM than at other stages of lactation. Ear notches were sampled concurrently from all cows for BVD antigen capture ELISA testing. Neither the milk ELISA results (no cows > 90% second Ab binding on both milk tests) nor ear notch testing classified any cows as PI animals. The milk BVD test might be useful to the dairy industry as a practical and convenient test for screening herd replacements, especially when large numbers of lactating cows are purchased and mixed into different pens throughout a dairy herd.